Detection of Rota and Corona viral antigens in diarrheic newly born calves in Menofiya governorate

The present study was carried out to determine the prevalence of Rota and Corona viruses infection
among newly born calves in Menofiya governorate during the period from November 2014 to March
2015. Two hundreds diarrhoeic stool samples were collected directly from newly born calves (aged from
1 to 30 days) in different localities of Menofiya governorate and were screened for Rota and corona
viruses using direct sandwich ELISA. The samples were grouped according to the age of the calves into
3 age groups as follows: 1^st age group (1-10 days), 2^nd age group (11-20 days) and 3^rd age group (21-30
days). Out of examined samples, were 87 (43.5%) positive for presence of Rotavirus antigen and 51
(25.5%) for coronavirus antigen; whereas 12 (6%) samples were positive for both viruses (mixed
infection). Rotavirus was found in high prevalence in the 1^st age group (60.8%) whereas Coronavirus
was found in high prevalence in the 3^rd age group (48.2%). Both viruses were found in the 2^nd age group
where the incidence of Rota was 40 % and Corona was 26.2%. Indeed, the study reports the circulation
of Rota and Corona viruses associating with diarrhea in newly born calves in the area of investigation.