Molecular and conventional methods for detection of Campylobacter spp. in retail poultry cuts, offal and chicken products

Authors

1 Food Control Dept., Fac. Vet. Med., Benha Univ.

2 Animal Health Research Institute, Shebin El koom Branch

Abstract

The goal of this study is to determine the prevalence of Campylobacter spp. in some poultry products by using both conventional and molecular methods. Two hundred chicken product samples were collected from various supermarkets at Menofia government (25g of each) of chicken breast with skin & without skin, thigh with skin &without skin, liver, gizzard, nuggets and luncheon. Most samples were contaminatedwith Campylobacter spp. except chicken luncheon. Chicken liver showed the highest contamination (92%) followed by gizzard(80%), thigh with skin (72%), thigh without skin (68%), breast with skin (60%) and breast without skin (48%). The lowest contamination was Chicken nuggets of 20 %. Campylobacter.jejuni (33.1%) was the most isolated serotype, and also followed by C.coli(16.6%), C.lari (6.9%), C.cinaedi(1.7%)  , C.butzieri (1.7%) and  C.upsaliens(1.1%)  . hipO  gene was detected  in isolated C. jejuni.Virulence genes cdtA, cdtB and cdt C , cdtA and cdtC ,cdtB and cdtC ,CdtA, CdtB  and CdtC were 30% , 15%,25%, 10% ,15 and 5 of isolated strains of C.jejuni, respectively. Finally the application of molecular methods is more favorable than conventional methods in detection of Campylobacter serotypes.

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