Bacteriological Profile of Frozen Chicken Meat Cuts at Qalubiya Governorate Markets
Fatin
Hassanin
Deprtment of Food Control and Hygiene, Faculty of Veterinary Medicine, Benha University
author
Fahim
Shaltout
Department of Food Control,
Faculty of Veterinary Medicine, Moshtohor,
Benha University, Egypt.
author
Ahmed
Maarouf
Animal health research institute (Benha Lab.), ARC
author
Suzan
Elsisi
Animal Health Research Institute (Benha Lab.), ARC
author
Ahmed
Yousef
Toukh Qalyubia meat Department Moshtohr veterinary medicine faculty’s
author
text
article
2020
eng
The study was conducted on 100 random samples of frozen chicken meat cuts represented by chicken wings, drumstick, thigh and breast (25 of each) purchased from different markets at Qalubiya Governorate, Egypt. The collected samples were bacteriological examination to investigate the bacteriological quality. The obtained results cleared thatthe mean values of APC, psychrotrophs; coliforms and S. aureus counts (CFU/g) of the wing samples were the most contaminated with such bacterial groups followed by drumstick, thigh, and breast samples. Further, bacteriological isolation of some food poisoning bacteria revealed detection of E. coli and coagulase positive S. aureus (CoPSA) in 11, and 21% of the examined samples, respectively. On the other hand, Salmonella species could not be detected in any of the examined samples. Concerning detection and typing of some enterotoxigenic CoPSA, 5 isolates were randomly examined using SET- RPLA test and the results indicated detection of Staphylococcal enterotoxin A in 3 isolates (60%), while Staphylococcal enterotoxins C and D were detected in one isolate (20% of each). Therefore, the sources and public health significance as well as trials for control of such serious food poisoning bacteria were discussed.
Benha Veterinary Medical Journal
Benha University; Faculty of Veterinary Medicine
1110-6581
39
v.
2
no.
2020
1
5
https://bvmj.journals.ekb.eg/article_141630_9fab503d5cf3de5a03762cd5757be868.pdf
dx.doi.org/10.21608/bvmj.2020.41010.1261
Essential oils as microbial reductant
Abo Bakr
M. Edris
Food Quality Control Program (FQCP), Faculty of Veterinary Medicine, Benha University, Egypt
author
Amany
M. Abd El-salam
Food Quality Control Program (FQCP), Faculty of Veterinary Medicine, Benha University, Egypt
author
ali
badran
food quality control department veterinary medicine education of benha university
author
text
article
2020
eng
Essential oils are concentrated liquids of complex mixtures of volatile compounds and can be extracted from several plant organs. Essential oils are a good source of several bioactive compounds, which possess antioxidative and antimicrobial properties. In addition, some essential oils have been used as medicine. Furthermore, the uses of essential oils have received increasing attention as the natural additives for the shelf‐life extension of food products, due to the risk in using synthetic preservatives. Essential oils can be incorporated into packaging, in which they can provide multifunctions termed “active or smart packaging.” Those essential oils are able to modify the matrix of packaging materials, thereby rendering the improved properties. This review covers up‐to‐date literatures on essential oils including sources, chemical composition, extraction methods, bioactivities, and their applications, particularly with the emphasis on preservation and the shelf‐life extension of food products. Keywords: Essential oils , antioxidative , antimicrobial , shelf‐life and packaging .
Benha Veterinary Medical Journal
Benha University; Faculty of Veterinary Medicine
1110-6581
39
v.
2
no.
2020
6
9
https://bvmj.journals.ekb.eg/article_141632_696649d79e2645f1da089873dc9faf5f.pdf
dx.doi.org/10.21608/bvmj.2020.38584.1241
Bacterial hazards of ready to eat fish products.
Eslam
El-Gazzar
Food Hygiene & Control Department, Faculty of Veterinary Medicine, Moshtohor, Benha University, Egypt
author
Faten
Hassanien
Food Hygiene & Control Department, Faculty of Veterinary Medicine, Benha university
author
Nahla
Abou ELRoos
Animal Health Research Institute, Shebin
Al kom branch
author
text
article
2020
eng
A total of one hundred random samples of ready to eat (RTE) fish products represented by fried Oreochromis niloticus, grilled Oreochromis niloticus, smoked Herring and salted Sardine (25 of each) were collected from different fish markets and restaurants in Menofia governorate, Egypt. The aim of this study was to evaluate the bacterial quality of the examined samples through isolation and identification of Enteropathogenic E. coli, Coagulase +ve S. aureus and Vibrio parahaemolyticus. The incidence of Enteropathogenic E. coli were 24%, 32%, 36% and 50 % in the examined samples of fried O. niloticus, grilled O. niloticus, smoked Herring and salted Sardine, respectively. While, Coagulase +ve S. aureus was detected in 32%, 44%, 52% and 72 % of the examined samples of fried O. niloticus, grilled O. niloticus, smoked Herring and salted Sardine, respectively. Furthermore, V. parahaemolyticus was isolated only from 20% and 36 % of the examined samples of smoked Herring and salted Sardine, respectively. As conclusion, bacterial examination of ready to eat fish products samples is a sensitive indicator verifying the quality and good hygienic status of RTE fish products. Keywords: Bacterial hazards ; Fried O. niloticus ; Grilled O. niloticus ; Smoked Herring ; Salted Sardine
Benha Veterinary Medical Journal
Benha University; Faculty of Veterinary Medicine
1110-6581
39
v.
2
no.
2020
10
14
https://bvmj.journals.ekb.eg/article_141717_c9ae1fcda98cb7ecabcf32d61a14a74c.pdf
dx.doi.org/10.21608/bvmj.2020.25463.1181
Some epidemiological studies on brucellosis in dairy farms in Gharbia governorate .Egypt.
Shaban
Khalafallah
Hygiene and Animal Management Department, Faculty of Veterinary Medicine, Benha University.
author
Hoda
Zaki
Brucella Department, Animal Health Research Institute, Cairo, Egypt.
author
ayman
seada
hygiene veterinary medicine banha
author
text
article
2020
eng
This study was conducted on dairy farms in Gharbia governorate, Egypt. From January to December 2018 .The study included 240 dairy farms and 3775 serum sample were collected for serological tests.25 farms (10.42) were positive for brucellosis,132 serum sample were positive by serological test divided according to production stage of animals as 121/3000(4.033%), 8/600 (1.33%) and 2/175 (1.142%) in cows ,heifers and bulls respectively. The result of culturing and confirmation by PCR indicated that Brucella melitensis biovar 3 is the dominant strain in dairy farms in Gharbia governorate. The spatial distribution of positive cases indicated that districts of Samnood, Kfr- Elzyat and Basion had the higher percent of positive cases as 6.35%, 4.67% and 3.57% respectively, however districts of El-Santa, Zefta and El-Mahla El-Kobra had the lowest percent as 1.49%, 2.3% and 2.89% respectively. The obtained result proved that, brucellosis is endemic in Gharbia governorate and good control program should be conducted to eradicate the disease.
Benha Veterinary Medical Journal
Benha University; Faculty of Veterinary Medicine
1110-6581
39
v.
2
no.
2020
15
19
https://bvmj.journals.ekb.eg/article_141647_69d2c561a6f1c8d2c88c6cc7dbe58c02.pdf
dx.doi.org/10.21608/bvmj.2020.39010.1243
Histopathological approach to interstitial Lung Diseases in camel in Slaughter houses, Aswan - Egypt.
Marawah
Ahmed
Faculty of Veterinary Medicine, Aswan University
author
text
article
2020
eng
Interstitial lung disease includes a wide range of pulmonary diseases associated with varying clinical appearance, therapeutic response and prognosis which are a real challenge for diagnoses. The aim of this study is to described the different histopathological patterns in interstitial pneumonia of camels slaughtered in Draw abattoir in Aswan government to provide clinicians with information on the pathological features of interstitial pneumonia, which in most cases may allow for a specific diagnosis and a careful correlation with clinical findings. Also, a pattern-based histopathological approach to interstitial lung disease can help guide clinicians in choosing suitable treatment and provide camel owners with correct prognoses. A total of 42 lungs of camels were examined for histopathological changes. 30 cases of Interstitial pneumonia were detected among the 42 examined camels’ lungs. Interstitial pneumonia in camels has variable pathologic pictures. Fibrotic and cellular interstitial pneumonia are considered as the two main histologic subtypes. Nodular patterns were recognized as uncommon histologic changes. This study concluded that cellular, fibrotic and nodular forms are the common patterns of interstitial pneumonia in camels
Benha Veterinary Medical Journal
Benha University; Faculty of Veterinary Medicine
1110-6581
39
v.
2
no.
2020
20
23
https://bvmj.journals.ekb.eg/article_141709_dd135d41f7b5ac2ec96f82f7aad8d20f.pdf
dx.doi.org/10.21608/bvmj.2020.39478.1262
Microbial Aspect of some processed meat products with special reference to aflatoxins
Khalifa
Abuzaid
HSEQ Supervisor in TSEBO EGYPT
author
Fahim
Shaltout
Food Hygiene and control Department, Faculty of Veterinary Medicine, Benha University
author
Ramadan
Salem
Department of Mycology, Animal Health Research Institute Dokki, Giza (ARC)
author
Eman
El-Diasty
Mycology Department, Animal Health Research Institute Dokki, Giza.
author
text
article
2020
eng
This work was carried out to evaluate the microbial aspect of some processed meat products sold in local markets in Kaliobia governorate with special reference to aflatoxin. Eighty samples of random meat products, sausages and Kofta (40 of each) were purchased from different shops at Kaliobia Governorate. All examined for their microbiological criteria. All the examined samples found to be contaminated with different types of microorganisms with the mean values of 11×106 ± 5.4×106.,and 2.04×106 ± 0.12×106 respectively for total aerobic bacterial counts; 6.7×103± 0.3×103,and 1.2×103 ±0.16×103 respectively for total coliforms;1.1x 103 ± 0.14 x 103 and 1.4 x 103 ± 0.27 x 103, respectively for total mould count;and 0.52 x 103 ± .08 x 103 and 0.47 x 103 ± .07 x 103 total yeast count. The incidence of mould in the examined sausage was higher than kofta samples.The average conc. of aflatoxins in sausage was 19.8±2.5a with frequency and incidence of 13 (32.5%), while in kofta was 11.5 ±3.3a with frequency and incidence of 12 (30%). Aspergillus and Penicillium species were the most prevalent species recovered from the examined meat product samples. The isolated A. flavus strains were screened for aflatoxin production. The public health significance of isolated microorganisms as well as recommended hygienic measures to keep meat products safe were discussed as the result showed that the unhygienic and poor sanitary conditions of meat products.
Benha Veterinary Medical Journal
Benha University; Faculty of Veterinary Medicine
1110-6581
39
v.
2
no.
2020
24
28
https://bvmj.journals.ekb.eg/article_141714_77c5b01a566a2ff5aa28ad4b02d6e974.pdf
dx.doi.org/10.21608/bvmj.2020.44886.1274
Chemical residues in ready to eat fish products
Eslam
El-Gazzar
Food Hygiene & Control Department, Faculty of Veterinary Medicine, Moshtohor, Benha University, Egypt
author
Mohamed
Hassan
Food Hygiene & Control Department, Faculty of Veterinary Medicine, Banha university
author
Faten
Hassanien
Food Hygiene & Control Department, Faculty of Veterinary Medicine, Benha University
author
Nahla
Abou ELRoos
Food Hygiene, Animal Health Research Institute, Shebin Al kom branch, Egypt
author
text
article
2020
eng
A total of one hundred random samples of ready to eat (RTE) fish products represented by fried Oreochromis niloticus, grilled Oreochromis niloticus, smoked Herring and salted Sardine (25 of each) were collected from different fish markets and restaurants in Menofia governorate, Egypt. The aim of this study was to estimate the concentration levels of chemical residues such as heavy metals residues (mercury, lead and cadmium) and pesticides residues (aldrin and malathion) through subjection the examined samples to atomic absorption spectrophotometer for detecting the residues of mercury, lead and cadmium and gas chromatography for the residues of aldrin and malathion. The mean values of mercury (mg/Kg) in fried O. niloticus, grilled O. niloticus, smoked herring and salted sardine were 0.43±0.01, 0.59±0.01, 0.70±0.01 and 0.94±0.02, respectively. The mean values of lead (mg/Kg) in fried O. niloticus, grilled O. niloticus, smoked herring and salted sardine were 0.27±0.01, 0.33±0.01, 0.45±0.01 and 0.61±0.01, respectively. The mean values of cadmium (mg/Kg) in fried O. niloticus, grilled O. niloticus, smoked herring and salted sardine were 0.11±0.01, 0.14±0.01, 0.20±0.01 and 0.29±0.01, respectively. The mean values of aldrin (ppb) only detected in fried O. niloticus, grilled O. niloticus and salted sardine were 82.95±4.19, 151.26±7.40 and 193.02±8.81, respectively. The mean values of malathion (ppb) only detected in grilled O. niloticus and salted sardine were 96.57 and 248.19±10.32, respectively. As conclusion, chemical examination of RTE fish products is a sensitive indicator verifying the quality and good hygienic status of RTE fish products. Keywords: Mercury; Lead; Cadmium; Aldrin; Malathion
Benha Veterinary Medical Journal
Benha University; Faculty of Veterinary Medicine
1110-6581
39
v.
2
no.
2020
29
33
https://bvmj.journals.ekb.eg/article_141643_05279381041df4f7ff64bec61718b4c4.pdf
dx.doi.org/10.21608/bvmj.2020.45268.1277
Assessment of pomegranate peel powder on microbial contamination of sausages
Khalifa
Abuzaid
HSEQ Supervisor in TSEBO EGYPT
author
Fahim
Shaltout
Department of Food Control,
Faculty of Veterinary Medicine, Moshtohor, Benha University
author
Ramadan
Salem
Mycology and mycotoxins department,
Animal Health Research Institute, Dokki-Giza
author
Eman
El-Diasty
Mycology Department, Animal Health Research Institute Dokki, Giza.
author
text
article
2020
eng
Growth of fungi on many types of foods under certain environmental conditions results in extensive spoilage of these foods due to production of off flavors, discolouration and bad tastes. Pomegranate (Punica granatum) peel is a nutrient-rich byproduct whose juice and related products are directly added to foods due to their pleasant taste, palatability, and preservative effects. This review aims to using of pomegranate peels powder at concentrations of 2g, 3g, and 5 g on physicochemical and chemical quality of sausage during a storage period at (1-4˚C) for 12 days. Also, microbiological criteria of sausage samples were recorded. Values of Total volatile nitrogen and Thiobarbituric acid were reduced by adding of different concentration of powders of pomegranate during refrigerated storage compared to control sausage samples. Phenolic compounds in pomegranate peels could have antimicrobial properties. So we can concluded that addition of pomegranate peel powder has an effect on lowering pH, TVN, TBA, APC, total coliforms, mould, and yeast counts in sausage.
Benha Veterinary Medical Journal
Benha University; Faculty of Veterinary Medicine
1110-6581
39
v.
2
no.
2020
34
39
https://bvmj.journals.ekb.eg/article_141728_5c100931c32f97a22f92482c9efa9a2b.pdf
dx.doi.org/10.21608/bvmj.2020.45919.1278
Control of some septicemic bacterial infections in Oreochromis niloticus, with special reference to their antimicrobial resistant genes.
Hadeer
Yousef
Department of Aquatic animals diseases and management, faculty of veterinary medicine, Benha university
author
Amel
El Asely
Department of Aquatic animals diseases and Management, Faculty of veterinary medicine, Benha university
author
Eman
Abdel Gawad
Department of Aquatic Animals Diseases and Management, Faculty of Veterinary Medicine, Benha University, Egypt.
author
Amany
Abbass
Department of Aquatic Animals Diseases and Management, Faculty of Veterinary Medicine, Benha University, Egypt.
author
Adel
Shaheen
Department of Aquatic animals diseases and management, faculty of veterinary medicine, benha university
author
text
article
2020
eng
The current study aimed to control the bacterial infection associated with summer mortalities in Nile tilapia. For determination to the sensitivity of the isolated bacteria to different antimicrobials; Aeromonas Veronii (A. veronii) strian (HY1, HY2, HY3, HY4 and HY6), Citrobacter freundii (C. freundii), Proteus vulgaris (P. vulgaris) and pseudomonas flurosence (P. flurosence) isolates were tested for antimicrobial sensitivity and antimicrobial resistant genes. In order to verify the result of antibiotic sensitivity test; experimental trial was performed to determine the effect of Florofenicol and Erythromycin against infection of O. niloticus with the different pathogenic bacteria (4 strains of A. veroni (A(HY2), A(HY3), A(HY4) and A(HY6)), C. freundi, P. flourosence and P. vulgaris). The results revealed that all tested bacterial isolates were 100 % sensitive to florfenicol and erythromycin, 50% sensitive to gentamycin, 25 % sensitive to nalidixic acid and sulphamethoxazole-trimethoprim, while sensitivity to other antibiotic discs was 0%. In addition, all bacterial isolates were 100% resistant to oxytertracycline and tetracycline lincomycin, ofloxacillin and penicillin, 87.5% resistant to ampicillin, 75% resistant to sulphamethoxazole-trimethoprim, 62.5% for naldixic acid and 37.5% for gentamycin . The survivability of challenged medicated fish with erythromycin ranged from 45-91.7%. Where, the survival % in HY3 infected groups was the highest recording; 91.7% followed by C. freundii (87.5%), HY6 (83.3 %) and P. vulgaris (81.3%). It was concluded that both erythromycin and florfenicol proved effective in treating bacterial infections associated with summer mortality syndrome. Furthermore, a number ofbacteria have multiple drug resistance attributing to the misuse of antimicrobial agents.
Benha Veterinary Medical Journal
Benha University; Faculty of Veterinary Medicine
1110-6581
39
v.
2
no.
2020
40
46
https://bvmj.journals.ekb.eg/article_141871_f906e3e7ab7fffd2cb152def72ad2f18.pdf
dx.doi.org/10.21608/bvmj.2020.43087.1267
Phenotypic and genotypic characterization of pseudomonas species isolated from frozen meat.
Basma
Khalafallah
Banha university faculty of veterinary medicine
author
Ashraf
Abd El-Tawab
Bacteriology, Immunology, and Mycology, Faculty of Veterinary Medicine, Benha University,_ Moshtohor
author
Shaimaa
Nada
Animal health research institute of shebin elkoum branch
author
Manar
Elkhayat
Bacteriology, Immunology and Mycology department, Faculty of veterinary medicine, moshtohor
author
text
article
2020
eng
Imported frozen meat is often more heavily contaminated with different bacterial species than home slaughtered one. . In addition, meat is a good environment for spoilage due to chemical and enzymatic activities. One hundred samples of imported Brazilian frozen (50 frozen cubic meat and 50 minced meat) were collected from different supermarkets in El-Menoufia Governorate, Egypt to be examined bacteriologically for detection of phenotypic and genotypic of Pseudomonas species. The incidence of Pseudomonas species were (35/50) 70% in frozen cubic meat. While, the incidence of such organism in the examined frozen minced meat samples was 80% (40/50). Psychrotrophic bacterial count in the examined frozen cubic meat ranged from 6 x 10 2 to 1.9 x 10 5 with mean value 2.24 x 10 4 cfu/g .In addition Psychrotrophic bacterial count in frozen minced meat ranged from 7x102 to 9 x 10 5 with mean value 1.7 x 10 5 cfu/g .The incidence of identified Pseudomonas species (number and percentages) detected in the examined samples of frozen meat represented by Ps. areuginosa , Ps. fluorescence , Ps. diminuta , Ps putida and Ps. fragi were 15(30%), 40(80%), 8(16%), 5(10% )and 4(8%), respectively. Regarding the minced meat samples , The incidence of identified Ps. areuginosa , Ps. fluorescence , Ps. diminuta , Ps. putida , Ps. fragi were 20(40%), 45(90%), 5(10%) ,7(14%) and 8(16%), respectively. The Pseudomonas species were resistant to Oxacillin. They were sensitive to Gentamycin except Ps. fluorescence. PCR is rapid and reliable tool for identification of different bacterial species
Benha Veterinary Medical Journal
Benha University; Faculty of Veterinary Medicine
1110-6581
39
v.
2
no.
2020
47
51
https://bvmj.journals.ekb.eg/article_144784_3640880d32219d43cfe8b767668ec5b0.pdf
dx.doi.org/10.21608/bvmj.2020.46777.1285
Limit of detection of bacterial contaminant in living bacterial vaccine
Gina
Elamry
central laboratory for evaluation vetrinary biologics,agricultural research center,Giza
author
Marwa
Khedr
central laboratory for evaluation of vetrinary biologics,agricultural research center,Giza
author
text
article
2020
eng
A total number of 30 vaccine vials including 3 batches of 3 different types of live bacterial poultry vaccines (Mycoplasma red vaccine, Mycoplasma yellow vaccine and non colored Mycoplasma vaccine) were experimentally contaminated with 1,5,10 CFU of Escherichia coli reference strain (ATCC25992) /dose then subjected for sterility testing as referenced in world organization of animal health(OIE). The sterility is defined as the absence of viable microorganisms, includes viruses. It should be achieved by the use of aseptic techniques and validated sterilization methods, including heating, filtration, chemical treatments and irradiation that fit the intended purpose. Further dilution was carried out of vaccines showing negative results.Kappa index in different contamination levels in all tested vaccines after dilution was ranged between high Kappa index and almost total . In this study , it could be concluded that the applied testing method is appropriate for testing sterility of living bacterial poultry vaccines and it is fit to use
Benha Veterinary Medical Journal
Benha University; Faculty of Veterinary Medicine
1110-6581
39
v.
2
no.
2020
52
54
https://bvmj.journals.ekb.eg/article_141731_8558f1cad195c31a3537fa87db8f21ff.pdf
dx.doi.org/10.21608/bvmj.2020.42123.1265
Prevalence of E.coli and staphylococcus aureus in meat and chicken Meals
Doaa
Helal
Department of Nutrition, Benha University Hospital
author
Abobaker
Edris
Food Hygiene and Control Department, Faculty of Veterinary Medicine, Benha University
author
Marionet
Nasseif
3 Food Hygiene Department, Animal Health Research Institute, Benha Lab
author
Amina
Elrais
Food Hygiene and Control Department, Faculty of Veterinary Medicine, Benha University
author
text
article
2020
eng
This study was conducted on 100 random samples of cooked chicken and beef meat meals (50 of each) with 125 gm weight of each sample .The cooked chicken meat samples were represented by cooked breast and thigh (25samples of each) and 50 samples of cooked meat meals were collected from a governmental hospital in Kaloubia governorate To determine the bacterial content of the beef and chicken meals. The results of bacteriological examination for cooked chicken and meat meals recorded as 1 (4%) E.coli from cooked chicken breast samples,where 3 (12%)isolates E.coli from cooked chicken thigh samples, while 2 (4%)isolates from cooked meat .where serologically identified as O111:H4; O114: H21 from cooked chicken breast (one from each type). In addition, 3(50%) were isolated from cooked chicken thigh represented by 2 (33.3%) O26 and one (16.7%) O114:H21 .Moreover, 1(16.7%) O127:H6 E.coli from cooked meat. Also, the study revealed that 6(6%) isolates of S. aureus were isolated from examined chicken and meat samples represented by 2(8%) from cooked chicken breast, 3(12%) from cooked chicken thigh and 1(2%) from cooked meat.
Benha Veterinary Medical Journal
Benha University; Faculty of Veterinary Medicine
1110-6581
39
v.
2
no.
2020
55
58
https://bvmj.journals.ekb.eg/article_142256_11521611f928b9ec93ff195d0fb8b518.pdf
dx.doi.org/10.21608/bvmj.2020.43697.1270
Ultrasonographic, Clinical and Haemato-biochemical Evaluation of Rumino-cutaneous Fistula in buffaloes (Bubalus bubalis).
Ahmed
Khalil
Department of Veterinary Surgery, Anesthesiology and Radiology, College of Veterinary Medicine, Benha University, Benha, Egypt.
author
mohamed
Zeineldin
Department of Animal Medicine, Faculty of Veterinary Medicine, Benha University, Benha, Egypt
author
mona
El Shemy
Department of Clinical Pathology, Faculty of Veterinary Medicine, Benha University, Benha, Egypt
author
text
article
2020
eng
One of the most prevalent postoperative complications following rumenotomy is rumino-cutaneous fistulation, that can cause significant economic issue in ruminant. The aim of this study was to determine the clinical, hematobiochemical, and ultrasonographic variations in rumino-cutaneous fistulation before and after surgical correction in buffaloes. Five buffaloes with rumino-cutaneous fistulation and five clinically healthy buffaloes were evaluated in this study. Buffaloes with rumino-cutaneous fistulation exhibited reduced appetite, decreased rumen motility, scanty feces, and significant alterations in hematobiochemical parameters compared with clinically healthy control buffaloes. Ultrasonographically, buffaloes with rumino-cutaneous fistulation displayed a significantly lower rate of rumen contractions and a substantial increase in the thickness of ruminal and abdominal walls. Significant postoperative improvement in clinical, hematobiochemical, and ultrasonographic parameters in affected buffaloes was noted compared with clinically healthy buffaloes. In conclusion, rumino-cutaneous fistula is a common surgical complication of rumenotomy and rumen trocarization in buffaloes. Ultrasonography, clinical and hematobiochemical examinations are essential for the proper assessment of rumino-cutaneous fistulation before and after surgical correction in buffaloes.
Benha Veterinary Medical Journal
Benha University; Faculty of Veterinary Medicine
1110-6581
39
v.
2
no.
2020
59
67
https://bvmj.journals.ekb.eg/article_141735_8dc27be953e2d9610a90200dda2c3675.pdf
dx.doi.org/10.21608/bvmj.2020.43962.1271
Field, serological and biochemical evaluation of Bovine Ephemeral Fever and Rabies vaccines in cattle and baffaloes
Enas
Abd El baset
Veterinarian
author
Marawan
Marawan
Department of Animal Medicine( Infectious Diseases), Faculty of Veterinary Medicine, Benha University, Egypt
author
Mohamed
Gouda
Department of Animal Medicine( Infectious Diseases), Faculty of Veterinary Medicine, Benha University, Egypt
author
Faysal
arnaout
Department of Animal Medicine( Infectious Diseases), Faculty of Veterinary Medicine, Benha University, Egypt
author
Zinab
Salem
chief of researches and director of veterinary serum and vaccine research institute, Abbasia, Cairo
author
text
article
2020
eng
Family Rhabdoviride contains 2 genera Ephemerovirus and Lyssavirus which contain viruses responsible for two destructive diseases Bovine Ephemeral Fever (BEF) and rabies, respectively. Both diseases cause direct and indirect economic losses related to deaths, abortions, cost of treatment and prevention, zoonotic impact and restriction of animal movement. The main objective of this study is to evaluate the immune responses of cattle and buffalo vaccinated with BEF and rabies vaccines when administered separately in comparison with co vaccination. Serum samples were collected from 16 cattle and buffaloes (eight of each) then subjected to serum neutralization test SNT, serum biochemical, liver and kidney function for comparison according to the vaccinated groups. Each animal species divided into 4 groups (2 animals /group) group 1 was vaccinated by 2 doses of attenuated BEF vaccine inactivated at time of use with 2 weeks in between, group 2 was vaccinated by 1 dose of inactivated rabies vaccine, group 3 was vaccinated by rabies vaccine simultaneous with 1st dose of BEF vaccine and boostering dose of BEF vaccine after 2 weeks, group 4 not vaccinated and let to be control group. The results showed that both live attenuated BEF and inactivated cell culture rabies vaccines are safe because they didn’t harmful effect on liver and kidney functions, they are immunogenic as they lead to significant increase in total serum protein due to increase in globulin. Co-vaccination of both vaccines together has higher levels of specific antibodies against both BEF and rabies in all vaccinated animals.
Benha Veterinary Medical Journal
Benha University; Faculty of Veterinary Medicine
1110-6581
39
v.
2
no.
2020
68
74
https://bvmj.journals.ekb.eg/article_143098_243e63d8bf8830e8f3cf47645a96de0f.pdf
dx.doi.org/10.21608/bvmj.2020.46256.1281
Epidemiological studies on brucellosisin dairy farms in Nile delta, Egypt.
Shabaan
Khalafallah
Department of Veterinary Hygiene and Management, Faculty of Veterinary Medicine, Benha University.
author
Hoda
Zaki
Brucella department, Animal Health Research Institute, Cairo, Egypt
author
ayman
seada
Tanta laboratory branch, Animal Health Research institute, Egypt
author
text
article
2020
eng
The current study was applied from January to December 2019 to determine and identify the prevalence rateof bovine brucellosis and its correlated risk factors in dairy herds in Nile delta, Egypt. The study populations comprised of 300 dairy farms which including 4000 dairy cattle. Estimated results showed that, the prevalence of brucellosis in dairy cattle was 6.05 % that depended on the result of CFT. The univariate statistical analysis revealed that positive cases of brucellosis was clearly higher in cattle housed under the intensive management system, and animals in the extensive management system had lower prevalence (P <0.001). Moreover, there was a statistically correlation between brucellosis and the age of animals (P < 0.01) but correlation was weak with the number of labor (P > 0.05). Significant increasing of positive cases was parallel with the increasing of the size of herd (P < 0.05). Sero-positivity to brucellosis was significantly correlated with history of abortions or stillbirths. The results estimated that brucellosis is endemic and widely distributed disease in Nile delta, Egypt.
Benha Veterinary Medical Journal
Benha University; Faculty of Veterinary Medicine
1110-6581
39
v.
2
no.
2020
75
78
https://bvmj.journals.ekb.eg/article_143104_27028646f3bcc064dc7cdbeca19cb0f7.pdf
dx.doi.org/10.21608/bvmj.2020.46753.1284
Shiga toxins Producing E.coli in Meat Products by Multiplex PCR
Mohamed
Hassan
Food Hygiene and Control Dep., Fac. Vet. Med., Benha University
author
Reham
Amin
Food Hygiene and Control Dep., Fac. Vet. Med., Benha University
author
Nahla
Aboelroos
Animal Health Research Institute, Shebin El-koom
author
yasmeen
ibrahim
GOEIC
author
text
article
2020
eng
The current investigation was carried out for 100 random sample of meat products represented by frozen beef burger, frozen kofta, minced meat and oriental sausage (25 each) gathered from various shops and retail stores in Menofya governorate. The gathered samples were subjected to bacteriological examination for the isolation and identification of non O157 E.coli and E.coli O157 by using Conventional and recent techniques as multiplex PCR. By the conventional method the incidence of E.coli in the tested samples of beef burger, kofta, minced meat and oriental sausage was 6(24%), 9(36%) , 5(20%) and 11(44%) respectively. Multiplex-PCR technique was applied on 10 random meat product samples (3 negative and 7 positive for the isolation of E.coli by conventional method). M-PCR technique was applied in order to detect stx1 and stx2. In this study ,the M-PCR give negative result with all tested food samples. This study clarified that multiplex PCR may give negative results due to inhibitors that can be found in microbial DNA solutions extracted from meat sample or due to inhibitors which added during processing of meat products .
Benha Veterinary Medical Journal
Benha University; Faculty of Veterinary Medicine
1110-6581
39
v.
2
no.
2020
79
83
https://bvmj.journals.ekb.eg/article_143390_987e51f7ce3de6f962ea615bc24b68e4.pdf
dx.doi.org/10.21608/bvmj.2020.45051.1276
Molecular studies on some factors of E. coli isolated from avian products and infants with diarrhea
Ashraf
Abd El-Tawab
Bacteriology, Immunology and Mycology Dept., Fac. Vet. Med., Benha Univ.
author
Gamal
Hassb El-Naby
Animal Health Research, Tanta branch
author
Nashwa
Mowaf
Animal Health Research, Tanta branch
author
Manar
Al-Khayat
Bacteriology, Immunology and Mycology Dept., Fac. Vet. Med., Benha Univ.
author
text
article
2020
eng
Extraintestinal pathogenic E. coli (ExPEC) are facultative pathogens that are part of the normal human intestinal flora which classified into several types, uropathogenic E. coli (UPEC), neonatal meningitis E. coli (NMEC), sepsis-associated E. coli (SEPEC), and avian pathogenic E. coli (APEC) (Jolanta et al.,2019). The present study was performed on a total number of 150 samples from which 100 samples were collected from poultry products (Gizzard, Liver, Banih, and Nuggets) also 50 samples from infants stool at Gharbia Governorate for isolation of E.coli and detection of some virulence genes. The results revealed that 26 samples were positive for Escherichia coli, 11 from infants stool, 7 from poultry liver samples, 5 poultry gizzard , 2 Banih and 1 from Nuggets samples. Amikacin 30 and ciprofluxacin 5 (oxoid) were the highest efficiency antimicrobial agents used in vitro. Six E.coli isolates were serotyped and identified as O157, O128, O125 and O86a. PCR results showed that TraT and pic pathotypic genes ( at 307 bp and 572 bp) were detected in all, but only O157 and O125 have stx2 gene ( at 779 bp). All that indicate that E.coli strains isolated were extra-intestinal pathogenic strains.
Benha Veterinary Medical Journal
Benha University; Faculty of Veterinary Medicine
1110-6581
39
v.
2
no.
2020
84
89
https://bvmj.journals.ekb.eg/article_144742_077ce6c462c4b5ecc22ffa6d86706d8b.pdf
dx.doi.org/10.21608/bvmj.2020.49149.1292
Clinical and biochemical investigations on bacterial diarrhea in Egyptian Buffalo Calves
Sherif
El-Seadawy
Department of Animal Medicine, Faculty of Veterinary Medicine, Benha University.
author
Hossam El-Din
El-Attar
Department of Animal Medicine, Faculty of Veterinary Medicine, Benha University.
author
Heba
Elkhyat
Department of Animal Medicine, Faculty of Veterinary Medicine, Benha University.
author
Mahmoud
Helal
Department of Animal Medicine, Faculty of Veterinary Medicine, Benha University.
author
text
article
2020
eng
The objective of the present study was to evaluate clinico-biochemical changes occurred in bacterial diarrhea in Egyptian Buffalo calves less than three months old. The study was carried out on 50 buffalo calves in El-Galaa Military farm that located in Ismailia Governorate, 40 diarrheic calves aged 55-75 days with an average body weight (35±2.5) kg were detected and 10 apparently healthy calves were kept as a control group to investigate the clinico-biochemical profiles. E. coli mixed or co-infection of Acinetobacterbaumannii/Iwoffiiss species with Klebsiella and pseudomonas species were isolated from the diarrheic calves. Diarrheic calves show signs of anorexia, weakness, dullness, staggering movement, weight loss, pale mucous membranes with sunken eyes and dehydration. Analysis of clinical and biochemical profile of the diarrheic calves revealed significant increase in body temperature, respiratory rate, skin fold test, serum potassium, chloride, urea, creatinine, ALT, AST, GGT, triacylglycerols, cortisol, CRP and CPK withsignificant decrease in pulse rate, serum sodium, glucose and total protein concentrations.It could be concluded that, bacterial diarrhea has a severe negative impact on the clinical and biochemical profile of the affected buffalo calves.
Benha Veterinary Medical Journal
Benha University; Faculty of Veterinary Medicine
1110-6581
39
v.
2
no.
2020
90
94
https://bvmj.journals.ekb.eg/article_142284_17ecddbb5096cef96942674d649331b9.pdf
dx.doi.org/10.21608/bvmj.2020.51991.1309
Bacterial and Chemical Quality of Raw Meat and Ready-To-Eat Cooked Meat
Hemmat
Ibrahim
Food Hygiene & Control Dept., Fac. Vet. Med. Benha University
author
Nesreen
Eleiwa
Food Hygiene Dept., Animal Health Research Institute, Agriculture Research Center, Giza, Egypt
author
Heba
Desoki
Food Hygiene & Control Dept., Fac. Vet. Med. Benha University
author
text
article
2020
eng
A grand total of ninety random samples of raw meat, grilled kofta, and kabab (30 of each) were collected from different meat restaurants in Benha city, Kaliobia governorate, Egypt. The study aimed to determine the effect of grilling on the bacteriological quality of the examined samples through the detection of APC, Total Enterobacteriaceae count, Total Coliform count, isolation, and identification of Enteropathogenic E.coli and S.aureus. The mean values of APC, Total Enterobacteriaceae counts, and Total Coliform counts of the examined samples were 3.25×104± 0.47×104, 2.45×104± 0.31×104, and 1.37×104± 0.18×104 for raw meat, 1.09×104± 0.22×104, 7.96×103± 1.58×103, and 4.14×103± 0.79×103 for grilled kofta, and 7.42×103± 1.05×103, 4.64×103± 0.70×103, and 2.29×103± 0.43×103 for kabab, respectively. The incidence of EPEC was 30%, 10%, and 3.3% in the examined samples of raw meat, grilled kofta, and kabab, respectively. S.aureus was detected in 26.7%, 6.7%, and 3.3% of the examined samples of raw meat, grilled kofta, and kabab, respectively. Also, the study aimed to determine their chemical quality through detection of pH, TVN, and TBA values. In conclusion, the examined raw meat samples were more contaminated with the highest level of microorganisms compared to those of grilled meat products and the bacteriological and chemical examination of those samples is a good indicator to detect the quality and hygienic condition of them.
Benha Veterinary Medical Journal
Benha University; Faculty of Veterinary Medicine
1110-6581
39
v.
2
no.
2020
95
99
https://bvmj.journals.ekb.eg/article_143380_c6a5a7e41bb1f485e481e97fd08f2e77.pdf
dx.doi.org/10.21608/bvmj.2020.52112.1310
Star Anise (Illicium verum) oil administration alleviates carbon tetrachloride-induced hepatotoxicity in rats
Eman
Ismail Ibrahim
Clinical pathology department, Faculty of veterinary medicine, Benha university
author
Ayman
Farid
Clinical Pathology Department, Faculty of Veterinary Medicine, Benha University
author
Ebtesam
enafie
Department of zoology, faculty of science, benha university
author
text
article
2020
eng
Abstract: The purpose of this work was to evaluate the protective effect of star anise oil against carbon tetrachloride (CCL4)-induced hepatic injury and oxidative stress in rats. Twenty-four male Wistar rats were divided into four equal groups. Group I (control): rats administered corn oil1 ml/kg orally every day for 5 weeks. Group II (star anise oil exposed group): rats received (1 ml/kg orally) every day via gastric tube over a period of 5 weeks. Group III (carbon tetrachloride): rats were injected IP with CCl4 (1:3 in corn oil, 2.5 ml/Kg, 3 doses at 72 hr interval) for 5 weeks. Group IV (CCl4 injected rats plus star anise oil) rats daily received star anise oil (1 ml/kg orally)plus CCl4 (as in group III).Obtained results showed significant increase in serum ALT, AST and ALP activities, decrease in total protein, albumin and globulin concentrations and significant increase liver tissue MDA level in CCL4 exposed rats. In addition , a significant decrease in GSH and CAT concentrations were recorded when compared with control group. Administration of star anise oil with CCL4 induced significant improvement of all previous parameters towards their normal ranges. Various histopathological alterations were detected in the liver treated with CCL4. Interestingly, rats treated with star anise oil plus CCL4 showed marked reduction in these pathological alterations in comparison to CCL4 intoxicated rats. These results suggested that star anise oil has the potential to ameliorate CCL4 induced hepatic damage.
Benha Veterinary Medical Journal
Benha University; Faculty of Veterinary Medicine
1110-6581
39
v.
2
no.
2020
100
104
https://bvmj.journals.ekb.eg/article_142588_aaaf1fb0def70b1d64f91de32e334277.pdf
dx.doi.org/10.21608/bvmj.2020.51053.1303
Determination of histamine level in frozen fish in relation to bacterial contamination
Mohammed
Hassan
Food Hygiene and control Department, Faculty of Veterinary Medicine, Benha University
author
Safa
Hussam
Food Hygiene and control Department, Faculty of Veterinary Medicine, Benha University
author
text
article
2020
eng
Histamine is a member of compounds called biogenic amines unremarkably made by chemical process of free amino acids and present in a many types of foods. Production of amine in concert of the biogenic amines (BA) in fish represents a health risk to human food intoxication. In this study ninety random frozen fish samples pictured by Mackerel, Saurus and Oreochromis niloticus (30 of each) were collected from markets in Kalyobia governorate to work out the microorganism count of coliform, staphylococci aureus and Enterobacteriaceae and examined for the presence of amine by assay. The results of medical specialty examination for frozen fish discovered that Enterobacteriaceae counts were recorded as 29 (96.7%) from frozen Mackerel samples, 26 (86.7%) from frozen Saurus samples and 24 (80%) from frozen Oreochromus niloticus . additionally, coliform counts were 27(90%) from frozen Mackerel samples,25 (83.3%) from frozen Saurus samples and 23(76.7) from frozen Oreochromus niloticus. Also, the study discovered that S. aureus counts were 22(73.3%) from examined Mackerel , 17(56.7%) from Saurus and 14(46.7%) from Oreochromus niloticus . mistreatment assay check, the results recorded that the amine mean values in examined fish samples were twenty nine.40 ± 0.31, 21.76 ±0.25 and 16.23±0.18 mg/kg for Mackerel, Saurus and Oreochromis niloticus severally. once and for all, the appliance of early detection of biogenic amines primarily amine manufacturing microorganism may scale back the health risk of amine intoxication associated fish and fish product microbic deterioration. Improvement of a selective medium to observe decarboxylating microorganism could also be a valuable tool.
Benha Veterinary Medical Journal
Benha University; Faculty of Veterinary Medicine
1110-6581
39
v.
2
no.
2020
105
110
https://bvmj.journals.ekb.eg/article_143069_6dfe4623b009e90eb54c0f58b26b9086.pdf
dx.doi.org/10.21608/bvmj.2020.51838.1307
Epidemiology and control of mange in sheep and goat in menoufia governorate
Esraa
Mohamed
Veterinary Clinic, Ostbarry Village, Shebin El-Kom, El-Menofia
author
S.S.
Khalafallah
Department of Veterinary Hygiene and Management, Faculty of Veterinary Medicine, Benha University.
author
Y.F.
Metawea
Department of Veterinary Hygiene and Management, Faculty of Veterinary Medicine, Benha University.
author
text
article
2020
eng
The present study aimed to investigate the epidemiology of mange disease in Menofia governorate and to identify the efficacy of different acaricides to control mange mites infesting sheep and goat in the governorate. For this purpose, a total number of 1500 sheep and 750 goats were examined during the different months and seasons of the year by visual inspection of animals’ body. The examined animals were also parasitologically and microscopically examined. All parasitological positive samples were subjected to different treatment protocols with acaricides. The results revealed that prevalence of mange in sheep was 24.5% and 23.33% in goat and the highest prevalence of mange in sheep and goat was in summer season, (36.57%) in sheep and (32%) in goat, while the lowest prevalence was in winter, (14%) in sheep and (14.8%) in goat. Moreover, the highest prevalence of mange was observed in Shebein El-Kom , (6.73% , 5.73% ), in sheep and goat, respectively, while the lowest prevalence was noticed in El-Bagour (4% , 3.73%), in sheep and goat, respectively. On the other hand, the results revealed that the efficacy of deltamethrine was 63.55%, the diazinon efficacy was 81.2%, the ivermectin efficacy was 81.95%, On the other hand, the treatment protocol of infested animals with deltamethrine + ivermectin proved an efficacy of 87.63% and the efficacy of the treatment protocol with diazinon + ivermectin was 94.7%.
Benha Veterinary Medical Journal
Benha University; Faculty of Veterinary Medicine
1110-6581
39
v.
2
no.
2020
111
116
https://bvmj.journals.ekb.eg/article_143103_8846f80b62c89c56f6809c9667815a76.pdf
dx.doi.org/10.21608/bvmj.2020.53293.1313
Phenotypic detection of methicillin-resistant Staphylococcus aureus in some cheese varieties
Ahmed
Abdel Khalek
Veterinary officer At Egyptian armed forces
author
marwa
Saad
Food control Dept., Fac. Vet. Med., Menoufiya University
author
Ahmed
Maarouf
Animal health research institute, ARC
author
Adham
Abdou
Food Hygiene and control Department, Faculty of Veterinary Medicine, Benha University.
author
text
article
2020
eng
Hundred and eighty random cheese samples, includes varities of cheese, soft (kareish and white); hard (Roomy); processed (Flamingo), beside machine and hand swabs were collected from different supermarkets (30 of each), at Qalubiya governorate, Egypt to investigate the incidence of Staphylococci with especial reference to MRSA strains. The results revealed that the mean value of Staphylococci and S. aureus counts (cfu/g) in kareish cheese samples were 3.16×105 and 6.57×104, respectively; for white cheese samples were 7.60×104 and 5.12×104, respectively; for Roomy cheese samples were 5.79×104 and 3.38×104, respectively; for Flamingo cheese samples were 2.96×104 and 1.05×104, respectively; for machine swab samples were 1.02×104 and 1.48×103, respectively and for hand swab samples were 1.43×104 and 3.71×103 (CFU/ml), respectively. A total of 118/180 (65.5%) isolates of staphylococci species, includes 55 S. aureus (30.6%). All 55 isolated S. aureus strains were coagulase positive while, other isolated staphylococcal strains (63) were coagulase negative. The isolated S. aureus was highly resistant for methicillin followed by oxacillin; nalidixic acid; ampicillin; oxytetracycline; cefotaxime and streptomycin. Meanwhile, it was highly sensitive to vancomycin followed by norfloxacin; gentamicin; meropenem and ciprofloxacin. A total of 49 S. aureus strains out of 55 ones were grown well on ORSAB media (they were MRSA strains). Finally, S. aureus and MRSA strains were found in the examined cheese sampels sold in markets at Qalubuiya governorate; beside that MRSA strains were detected that threats the consumer's health.
Benha Veterinary Medical Journal
Benha University; Faculty of Veterinary Medicine
1110-6581
39
v.
2
no.
2020
117
121
https://bvmj.journals.ekb.eg/article_143073_4d08ae84fa2f5ce13588b229d8fa9ee7.pdf
dx.doi.org/10.21608/bvmj.2020.50132.1296
Prevalence of Some Foodborne Pathogens in Some Ready to Eat Meats
Mohammed
Elheity
General Organization for Imports and Exports Control, International Cairo Airport Branch
author
Mohamed
Haasan
Food Hygiene and Control Dept., Fac. Vet. Med., Benha University.
author
Reham
Amin
Food Hygiene and Control Dept., Fac. Vet. Med., Benha University.
author
text
article
2020
eng
This study was conducted on 90 random samples of cooked chicken and beef meats (45 of each) with 125 gm weight of each sample .The beef meat samples were represented by beef kabab , beef kofta and shawerma(15 samples of each) while the chicken meats samples were represented by fried pane , fried drum sticks and chicken shawerma (15samples of each ) which were collected from Kafr Elzayat city , Gharbia governorate to evaluate the bacteriological quality of ready to eat chicken meats and beef meats . The results of bacteriological examination for meat and chicken recorded that E.coli were isolated as 3 from beef kabab samples, 4 from grilled beef kofta samples, 7 isolates from beef shawerma. E.coli isolated from fried chicken pane were 4, 6 isolates from fried chicken drum stick samples and 9 isolates from chicken shawerma. The study revealed the edibility of samples based on Staph.aureus count that 1, 3 and 4 samples from beef kabab , kofta and beef shawerma respectively were not accepted while in chicken samples pane , drum stick and chicken shawerma recorded 2 ,5 and 7 respectively were not accepted . S.Entertidis was isolated from grilled beef kofta (6.67%), beef shawerma (13.33%), chicken pane (6.67%) and chicken shawerma (6.67%). Also S.typhimurium was isolated from 6.67%, 13.33 %, 6.67%, 6.67% and 6.67% of the examined samples of beef kabab, beef kofta, beef shawerma, chicken drum stick and chicken shawerma , chicken drum stick (6.67%) and chicken shawerma (20%).
Benha Veterinary Medical Journal
Benha University; Faculty of Veterinary Medicine
1110-6581
39
v.
2
no.
2020
122
126
https://bvmj.journals.ekb.eg/article_141944_b9c10920a9d43fc79936b907234de576.pdf
dx.doi.org/10.21608/bvmj.2020.49322.1293
Effect of dietary nucleotide supplementation on broiler intestinal histomorphology
Nasser
Khedr
Department of Nutrition and Clinical Nutrition, Faculty of Veterinary Medicine, Benha University. Egypt.
author
Tahia
Ahmed
Department of Nutrition and Clinical Nutrition, Faculty of Veterinary Medicine, Benha University. Egypt.
author
sarah
nagiub
faculty of veterinary medicine, El-Mansoura university
author
text
article
2020
eng
The present experiment was performed to investigate the influence of two different products of nucleotides as a feed additive for broiler chicks in terms of improvement of intestinal histomorphology. A total number of two hundred and seven-one-day-old broiler chicks (Ross 308) were randomly distributed into three different treatment groups (A, B, and C), of three replicates (23 chicks / replicate). The chicks in control group (A) were fed negative control diet, group B were fed diet containing (Nucleoforce®) (0.025%) while group C were fed diet containing (Immune force®) (0.1%). Results showed that, groups fed diet supplemented with nucleotides had expressed significant increase in length of intestinal villi when compared with the control one, although group B that was fed Nucleoforce® showed highest values among the three different groups. It was concluded that, inclusion of nucleotides (Nucleoforce® and Immune-force®) in broiler diets had a positive role in improvement of intestinal histomorphology.
Benha Veterinary Medical Journal
Benha University; Faculty of Veterinary Medicine
1110-6581
39
v.
2
no.
2020
127
131
https://bvmj.journals.ekb.eg/article_141942_04464721002a2ec88f568ad36c3b5e08.pdf
dx.doi.org/10.21608/bvmj.2020.49900.1295
Molecular matching of circulating foot and mouth disease viruses and vaccinal strains in Egypt, 2016-2019.
Nehal
Shahen
Animal Health Research Institute, Benha branch, Egypt
author
zeinab
aboezz
Virology department, faculty of vet medicine, Benha university.
author
Ayman
Elhabba
Department of Virology, Faculty of Veterinary Medicine, Moshtohor, Benha University.
author
Naglaa
Hagaa
Animal Health Research Institute, Benha branch, Egypt
author
Saad
Sharawi
Department of Virology, Faculty of Veterinary Medicine, Moshtohor, Benha University.
author
text
article
2020
eng
Regardless mass vaccination programs in Egypt with inactivated polyvalent vaccines of FMDvirus. FMD virus is still persistent with major economic burden. Serotypes of FMD namely, A, O, and South African Territories (SAT2) are endemic with repeated outbreaks of new linage. So selection of appropriate vaccine is very difficult. In this study; Phylogenetic analysis of VP1 genome for the existing serotype (A, O, SAT2) between 2016 to 2019 approved circulation of serotype O of East Africa 3 (EA-3) topoype, AIran05 lineage and A Africa G-IV lineage of serotype A and topotype VII of serotype SAT2 including Alx-12 and Lib-12 lineages. Significant amino acid changes in critical antigenic sites between field isolates and vaccine strains were observed. The present findings indicate the importance of an active routine surveillance system incorporating antigenic and genetic analysis designated to continually update information about field isolates of FMDV. Such as this protocols might be affected on FMD strategy control in Egypt and surrounding country.
Benha Veterinary Medical Journal
Benha University; Faculty of Veterinary Medicine
1110-6581
39
v.
2
no.
2020
132
137
https://bvmj.journals.ekb.eg/article_144739_f959201e28a338780a3f09b2ef4698ba.pdf
dx.doi.org/10.21608/bvmj.2020.50573.1299
Application of Linear Discriminant Model to Evaluate the Association Between Milk Production, Reproductive Performance, and Calving Season in Dairy Cattle
hayaa
abdelrahman
Animal wealth department,faculty of veterinary medicine,Qlioup,Qliopia
author
Eman
Kamel
Animal Wealth Development Department, Faculty of veterinary medicine,Banha university
author
fardos
Hassan
Department of Animal Wealth Development, Faculty of Veterinary Medicine, Zagazig University,zagazig,sharkia
author
text
article
2020
eng
This study was aimed to determine the performance of linear discriminant analysis (LDA) for differentiation between seasons of calving on the basis of305day- milk yield, fat %, protein %, days open (DO), days to first insemination (DFI), and number of services per conception. By considering the assumption of this method, a random sample was selected from the animals being represented by all explanatory variables. The discrimination between seasons of calving was depended on the significance of coefficients, classification rate, in addition to the group centroids. Results showed that LDA method selected 305day-milk yield (kg), days open (DO), days to first insemination and number of services per conception, as the significant (P < 0.05) contributors for data classification. The total variance explained by 2 functions was 79.2% and 15.9%, respectively. So, the 1st function can do well in discrimination process than the 2ndone. The percentage of correct classification was 64.6%. In conclusion, LDA can be used effectively for classification of calving seasons, even with violation of normality assumption.
Benha Veterinary Medical Journal
Benha University; Faculty of Veterinary Medicine
1110-6581
39
v.
2
no.
2020
138
141
https://bvmj.journals.ekb.eg/article_141941_f268fb9f845a5d8d9fcce10fc841dabf.pdf
dx.doi.org/10.21608/bvmj.2020.50592.1300
Comparative Immunopathological Study of Various Intra-Cloacal Infectious Bursal Disease Vaccine Doses.
Mohamed
Abaza
Avian and Rabbit Diseases Department, Faculty of Veterinary Medicine, Benha University, Egypt
author
Ibrahim
ElBorai
Avian and Rabbit Diseases Department, Faculty of Veterinary Medicine, Benha University, Egypt
author
Ahmed
Eissa
Avian and Rabbit Diseases Department, Faculty of Veterinary Medicine, Benha University, Egypt
author
Kamel
zyan
Avian and Rabbit Diseases Department, Faculty of Veterinary Medicine, Benha University, Egypt
author
text
article
2020
eng
Infectious bursal disease (IBD) is a contagious immunosuppressive viral infection of young chickens. Intra-cloacal approach is effective for vaccinating chicks with high maternally derived IBD antibodies; however, the vaccinal traditional dose may be unsuitable for this route. A total of 60 one-day-old specific-pathogen-free (SPF) chicks were randomly distributed into four groups. Group-1: was control and chicks in group-2: were vaccinated with 103.5 TCID50 of D78 strain/ bird via intra-cloacal route, chicks in group-3: were vaccinated with a ten-fold higher dose (104.5 TCID50/bird) and chicks in group-4: were vaccinated with a dose of 102.5 TCID50 of D78 strain/bird using same approach. IBD antibody titers were measured at the 14th and 21st days of age. The immunosuppressive effects of different doses were determined through humoral immune response to avian influenza and Newcastle disease vaccines, the relative weight of primary lymphoid organs, and histopathologically evaluated bursal lesions. The results indicated that a dose even ten-fold higher than the usual dose to SPF chicks via intra-cloacal route caused neither morbidity nor mortality. All vaccine doses induced potent immune responses against IBD, but the highest IBD antibodies titers were observed in group-4 while IBD antibodies titers were decreased with increasing vaccine doses in other experimental groups. Even the vaccinated birds showed moderate histopathological lesions and the diminutive relative weight of bursae; there was no immunosuppressive effect in the immune response to other vaccines in group-4 and transient immunosuppressive effect in groups-2 and-3. All that proves the safety and effectiveness of various intra-cloacal vaccination approach doses.
Benha Veterinary Medical Journal
Benha University; Faculty of Veterinary Medicine
1110-6581
39
v.
2
no.
2020
142
146
https://bvmj.journals.ekb.eg/article_141712_07bf771ea0d4efb270d08fd518a589b1.pdf
dx.doi.org/10.21608/bvmj.2020.45986.1280
Effect of extracted LPS of Salmonella typhimurium on chicken immunity.
Ashraf
Abd El-Tawab
Bacteriology, Immunology and Mycology Department, Faculty of Veterinary Medicine, Benha University, Egypt.
author
Heba
Hassan
Animal Health Research Institute, Agriculture Research Center, Dokki, P.O. Box 246, Giza 12618, Egypt
author
Fatma
El-Hofy
author
Mai
Fadel
Animal Health Research Institute, Agriculture Research Center, Dokki, P.O. Box 246, Giza 12618, Egypt
author
Aml
Ezzat
Animal Health Research Institute, Agriculture Research Center, Dokki, P.O. Box 246, Giza 12618, Egypt
author
Enas
Soliman
Bacteriology, Immunology and Mycology Department, Faculty of Veterinary Medicine, Benha University, Egypt.
author
text
article
2020
eng
In this study, the prevalence of Salmonella typhimurium in Egyptian chicken farms was studied. Examination of 79 chicken farms distributed in Egypt governorates from 2017 to 2019. Salmonella was detected in broiler flocks 20 % positivity (18 out of 90). However, in layer flocks showed 70 % positivity (7 out of 10). Then Salmonella typhimurium was confirmed using Salmonella typhimurium STM4 495 gene using conventional PCR. Lipopolysaccharides (LPSs) are the major constituent of the bacterial Gram-negative outer membrane including Salmonella. (LPSs) is an immunodominant molecule that is important for the virulence and pathogenesis of Salmonella typhimurium which called endotoxin. Extraction and purification of LPS from isolated S. typhimurium using isopropanol and sodium hydroxide mixture this overcome the presence of nucleic acids of extracted Salmonella in the final product. The purity of LPS isolated from Salmonella typhimurium was assessed by HPLC showing highly purified extracted LPS. Effect of extracted LPS of Salmonella typhimurium on poultry immunity were studied via a biological experiment using Chicks of the broiler breeds to assess the response to LPS stimulation via in vivo experiment using Different concentrations of extracted LPS (50,100,150 and 200 μg/kg) were injected subcutaneously in 21-day old SPF chicks. After 3 hours of injection samples taken to assest of the effect of LPS on RNA expression of the immune-related genes; interferon-γ (IFN-γ) and interleukin 1β (IL-1β). There was significant upregulation of IFN-γ and low downregulation of IL-1β of the immune-related genes.
Benha Veterinary Medical Journal
Benha University; Faculty of Veterinary Medicine
1110-6581
39
v.
2
no.
2020
147
152
https://bvmj.journals.ekb.eg/article_144768_f28a3ef09708a2f774cb81ef57bece92.pdf
dx.doi.org/10.21608/bvmj.2020.46621.1283
Bacterial Status Of Broiler chicken Meat Meals Served at Governmental Hospital E
Heba
Desokey
Benha University Hospital
author
Abo Bakr
Edris
Food Hygiene and control Department, Faculty of Veterinary Medicine, Benha University
author
Islam
sabek
Food Hygiene and control Department, Faculty of Veterinary Medicine, Benha University
author
Ahmed
Abd-ElGhaffar
Microbiology Dept., Animal Health Research Institute, Benha Branch.
author
text
article
2020
eng
A grand total of 90 random samples with 250 gm weight of each sample of boiled, grilled and fried broiler chicken meat meals (30 of each) were collected from a governmental hospital at various times in Kalyobia governorate, Egypt were collected for microbiological examination .The average values of APC, Enterobacteriaceae, coliform and staphylococcalcounts were 4.81x104 0.65x104,2.16x1040.41x104cfu/g,1.45x1030..36x103and3.10x1030..48x103 in boiled chicken meat meals,9.97x1032.18x103,5.73x1030.96x103, 9.74x1022.07x102 and 1.26x1030.19x103cfu/g in grilled chicken meat meals, 6.02x1031.33x103.,1.81x1030.27x103,4.19x1020.53x102 and 7.58x1021.25x102cfu/g in fried chicken meat meals. Moreover, the incidence of serologically identified E. coli as Enteropathogenic E. coli (E. coli O1:H7 , E. coli O2: H6 , E. coli O78 , E. coli O124 , .E. coli O126: H21E. coli O128: H2and E. coli O146: H21), EnterotoxogenicE. coli (E. coli O2:H6 ,E. coli O78 , E. coli O158 , E. coli O128: H2 and E. coli O91: H21) Enteroheamorrhagic E. coli (E. coli O1:H7,E. coli O78 and E. coli O126:H21) and Enteroinvasive E. coli (E. coli O146 :H21). The public health importance of the isolated microorganisms and the recommended points were discussed. Keywords: Meat meals, Enterobacteriaceae , staphylococci, coliform.
Benha Veterinary Medical Journal
Benha University; Faculty of Veterinary Medicine
1110-6581
39
v.
2
no.
2020
153
157
https://bvmj.journals.ekb.eg/article_143062_251b5a1597b2e6d5a50d9b908f143911.pdf
dx.doi.org/10.21608/bvmj.2020.48666.1290
Lactic and Acetic Acids as Bacterial Decontaminators on Chicken Carcasses
abobakr
Edris
Food Hygiene and control Department, Faculty of Veterinary Medicine, Benha University
author
Reham
Amin
Food Hygiene and control Department, Faculty of Veterinary Medicine, Benha University
author
Nesreen
Eleiwa
Food Hygiene, Animal Health Research Institute (AHRI), Agriculture research center, Giza-Egypt
author
Ramy
Elnagar
Food Hygiene and control Department, Faculty of Veterinary Medicine, Benha University
author
text
article
2020
eng
This study was done to evaluate the efficiency of two decontaminators (Lactic and acetic acids) for decreasing the contamination level in chicken carcasses (cut up), which had been artificially inoculated with food poisoning microorganisms (Salmonella Enteritidis with infective dose 105and Staphylococcus aureus with infective dose 107). A total of 42 random samples chicken breast samples were collected from different markets in Gharbia governorate, Egypt. The collected chicken samples were then inoculated with the tested microorganisms Salmonella Enteritidis and Staphylococcus aureus (21 Samples for each microorganism). Thus, the samples were dipped into containers containing distilled water (Control), lactic acid 1%, 2%, 3% and acetic acid 0.5 %, 1 %, 1.5%for 20 seconds and 1 minute, respectively. The results indicated that the reduction % of acetic acid for Salmonella Enteritidis were 90.69%, 93.80%, 95.17% for 20 seconds and 91.39%, 96.56%, 96.90% for 1 minute, respectively. Regarding lactic acid, the reduction % was 61.43%, 94.49%, 97.41% for 20 seconds and 92.07%, 99.28%, 99.24% for 1 minute, respectively. While, the reduction of Staphylococcus aureus by acetic acid were 95.64%, 99.63%, 99.78% for 20 seconds and 97.84%, 99.94%, 99.98% for 1 minute but it was 99.88%, 99.98%, 99.99% for 20 seconds and 99.98%, 99.99%, 100% for 1 minute for lactic acid, respectively. Lactic acid with a concentration of 3% showed the highest reduction on Salmonella Enteritidis and Staphylococcus aureus. The effect of such organic acids on Gram positive bacteria as Staphylococcus aureus was higher than Gram negative ones as Salmonella Enteritidis
Benha Veterinary Medical Journal
Benha University; Faculty of Veterinary Medicine
1110-6581
39
v.
2
no.
2020
158
160
https://bvmj.journals.ekb.eg/article_145390_d812b8397c7470b3d96b360479f9b5b5.pdf
dx.doi.org/10.21608/bvmj.2020.50321.1297
Some studies on the reproductive performance in small ruminants
Ahmed
Gouda
Kafr Shokr Veterinary Administration, Qalyubia, Egypt
author
Mohamed
Kandiel
Dept. of Theriogenology, Faculty of Veterinary Medicine, Benha University, Egypt
author
Mohsen
Agag
Dept. of Theriogenology, Faculty of Veterinary Medicine, Benha University, Egypt
author
text
article
2020
eng
Abstract The current study entails the data obtained from ultrasonography investigation of the reproductive statuses of sheep (n=232) and she-goats (n=164) admitted to Meet Kenana, Veterinary Clinic, Tukh, Qalyubia governorate aiming to declare the contributing factors (species, season, body weight and age) impact their fertility. Pregnancy examination revealed higher rate of singleton pregnancy in ewes, while multiple pregnancy was higher in she-goats. A higher incidence of pregnancy was recorded during winter season (46.74%; 86/184), but lower pregnancy rate was recorded during summer season (5.98%; 11/184). In cyclic animals, a high incidence of follicular phase was recorded during spring season (43.22%), while luteal phase was high in winter (68.18%). On the other hand, in non-pregnant animals diagnosed to have inactive ovaries, higher incidence was recorded in sheep (10.34%; 24/232;) than she-Goats (6.70%; 11/164). On the other hand, uterine disorders at high incidence was recorded in in she-goats (9.75%; 16/164) than sheep (6.46%; 15/232). Summer season was associated with high rate of inactive ovaries (62.85%), while spring season was accompanied with high incidence of uterine disorders (70.96). Ewes at 60-70 kg body weight and 4-5 years old, and she-goats at 40-50 kg body weight and 2-3 years old showed an improved reproductive index (cyclicity and pregnancy rates). From the present study it could be concluded that the ultrasound is a promising diagnostic tool helps in improving the reproductive management of small ruminants through verifying the pregnant status, selection of fertile cyclic animals before breeding, and discarding the protracted infertile cases.
Benha Veterinary Medical Journal
Benha University; Faculty of Veterinary Medicine
1110-6581
39
v.
2
no.
2020
161
166
https://bvmj.journals.ekb.eg/article_141897_65c844a31e95a506551e0e8ffc455ef0.pdf
dx.doi.org/10.21608/bvmj.2020.55696.1324
Evaluation of renoprotective effect of grape seed proanthocyanidin extract on Cyclosporine A- induced Nephrotoxicity by mitigating inflammatory response, oxidative stress and apoptosis in rats
Samy
Hussein
Department of Biochemistry, Faculty of Vet. Med., Benha University, Egypt.
author
Yakout
Elsenosi
Department of Biochemistry, Faculty of Vet. Med., Benha University
author
Tahia
Esmael
nutrition and clinical nutrition, veterinary medicine, banha university, alzaqaziq, Egypt
author
Aziza
Amin
Pathology department, Faculty of Vet. Med., Benha Yniversity
author
Eman
Sarhan
Biochemistry department, Faculty of veterinary medicine, Beha University, Egypt.
author
text
article
2020
eng
Cyclosporine A (CsA) is a drug used as immunosuppressive agent in organ transplant and non-transplant medicine. The main secondary effect results from CsA treatment is nephrotoxicity. A protective effect of Grape seed proanthocyanidin extract (GSPE) against CsA-induced nephrotoxicty in rats was assessed. Thirty male rats were divided into three equal groups. Group I: (Normal control), received no drugs, Group II: (CsA treated), rats received oral dose of CsA (25 mg/kg b.wt/day) for 21 successive days. Group III: (GSPE protected+ CsA), rats received GSPE (200 mg/kg b.wt/day) orally 7 days before and during 21 days of CsA treatment. The obtained results showed a significant increase in serum urea and creatinine concentration in addition to L-MDA levels in kidney tissue while a marked decrease in renal catalase activity and GSH concentration in CsA treated rats. Moreover, a significant down-regulation in Bcl-2 and up-regulation of NF-κB, PAI-1, Caspase-3 and p53 gene expressions were observed in kidney tissues of CsA treated rats. Meanwhile, GSPE potentially improved renal function and oxidative alterations related to CsA near its normal ranges. Also, various histopathological alterations were detected in kidneys of CsA treated rats. Interestingly, histopathological findings supported that where GSPE markedly attenuated the harmful effects induced by CsA and protected kidney tissues. Our research could conclude that, GSPE has an ameliorating role as potent antioxidant, anti-inflammatory and anti-apoptotic agent via inhibition of inflammatory (NF-κB, PAI-1) and apoptotic (Caspase-3, p53) signaling pathway in modulation of CsA-induced nephrotoxicity.
Benha Veterinary Medical Journal
Benha University; Faculty of Veterinary Medicine
1110-6581
39
v.
2
no.
2020
167
172
https://bvmj.journals.ekb.eg/article_141890_03388e7db6e047dae1d65dc6bc5e15cd.pdf
dx.doi.org/10.21608/bvmj.2020.33392.1218
Chemical studies on shelf life time of chilled chicken meat
Ebtehal
Awad allah
Meat hygiene. Veterinary medicine banha university. Banha. Egypt
author
Saad
Saad
Department of food hygiene and control, Faculty of veterinary medicine, Benha University, Qalubia, Egypt
author
Mohamed
Hassan
Department of food Hygiene and control, Faculty of veterinary medicine, Benha university, Qalubia, Egypt
author
Hala
Hassan
Department of food hygiene, Animal Health research institute, Dokki, Giza, Egypt
author
text
article
2020
eng
Different trails were applied to chicken meat to prolong their shelf life by adding spices such as clove, ginger, soy sauce and mixture of them and keep the sample at 4 degree C at the refrigerator and the samples were examined 2 days interval till it's decomposition. A total of 180 chicken samples divided into control samples and samples treated with spices were analyzed for determination of keeping quality tests as: pH, thiobarbituric acid (TBA), total volatile base nitrogen (TVBN), and sensory evaluation (color, odor and taste) by score number provided by panelists. The results showed that the mean value of pH, TBA, TVBN in all the treated samples through all the storage period (1st, 3rd, 5th, 7th, 9th and 11th) were significantly (P < 0.05) lower than the control samples with best results in the mixture spiced samples in the keeping quality indices, and higher color, odor and taste scores in the treated samples than the control, which resulted in extension of the shelf life till 11 days in the treated samples, while in the mixture samples exceeds 11 days, however the control sample reaches 7 days only. These results concluded that spices are very important as a natural antioxidant against lipid oxidation in chicken samples. So, it needs more attention in the processing of chicken and meat products.
Benha Veterinary Medical Journal
Benha University; Faculty of Veterinary Medicine
1110-6581
39
v.
2
no.
2020
173
176
https://bvmj.journals.ekb.eg/article_141869_2d77b17f65e46ec3801aee8f7fdc0689.pdf
dx.doi.org/10.21608/bvmj.2020.36564.1227
Effect of sodium tripolyphosphate on the bacteriological quality of refrigerated Nile tilapia fillets
Hemmat
Ibrahim
Food Hygiene and control Department, Faculty of Veterinary Medicine, Benha University
author
Mohamed
Hassan
Food Hygiene and control Department, Faculty of Veterinary Medicine, Benha University
author
Reham
Amin
Food Hygiene and control Department, Faculty of Veterinary Medicine, Benha University
author
Omayma
Maghraby
Food Hygiene Dept., Animal Health Research Institute, Dokki.
author
ahmed
mostafa
food hygiene department, Animal Health research Institute, Dokki.
author
text
article
2020
eng
The effect of different concentrations of sodium tripolyphosphate on the bacteriological quality of refrigerated Nile tilapia fillets was determined. There were significant differences (P<0.05) among control with different concentrations of STPP treated samples on Total Aerobic plate count (APC) and the psychrotrophic bacteria counts. The control and 2%STPP treated samples exceeds the maximum permissible limit 6 log of APC after 6th and 9th day, respectively. While both 5%STPP and 10%STPP treated samples exceeds this limit after 12th day. Non-significant differences (P>0.05) were detected among all examined samples at 0 day, 3rd, 6th or at 15th day on total coliform count. The highest reduction % of APC, total psychrotrophic count and total coliform count was (26.62% at the 3rd day, 32.99% at the 9th day and 20% at the 6th day) of 10% STPP treated samples, respectively. This study shows that, STPP have anti-microbial effect on APC and psychrophilic count but it has no effect on coliform bacteria. And it helps in extension of Nile tilapia fillets shelf life during refrigeration storage.
Benha Veterinary Medical Journal
Benha University; Faculty of Veterinary Medicine
1110-6581
39
v.
2
no.
2020
177
180
https://bvmj.journals.ekb.eg/article_143054_2ffb869aebee4535e6ed00a45c4c9daf.pdf
dx.doi.org/10.21608/bvmj.2020.39498.1251