Estimation of lead and copper residues in sheep, goat milks and Karish cheese
Seham
El-Badry
Educational Veterinary Hospital, Faculty of Veterinary Medicine, Zagazig University, Zagazig, Egypt
author
Amal
Raslan
Educational Veterinary Hospital, Faculty of Veterinary Medicine, Zagazig University, Zagazig, Egypt
author
text
article
2016
eng
Dairy products are considered as one of important foods in the human diet. They are good source of protein, fat,and major minerals. Heavy metals are persistent toxic chemicals that have a public health concern. Milk of smallruminants such as sheep and goat is considered as a very important source for animal-derived protein in manyArabian countries and Egypt. However, studying the heavy metal load in milk of small ruminant had received lessattention. Thus, this study aimed to estimate the residual concentrations of lead (Pb) and copper (Cu) in milk ofsheep, goat' milk and cheese manufactured from sheep milk. The public health importance of the existed metalswas also discussed. All examined samples had Pb concentrations higher than the maximum permissible limits(MPL) set by WHO/FAO. Similarly, goat milk samples had Cu concentrations higher than MPL, while in case ofsheep 70% and 90% of examined milk and cheese samples had Cu levels higher than MPL
Benha Veterinary Medical Journal
Benha University; Faculty of Veterinary Medicine
1110-6581
30
v.
2
no.
2016
1
5
https://bvmj.journals.ekb.eg/article_31318_2e0e51b1889067d8007d6f5e90589e1e.pdf
dx.doi.org/10.21608/bvmj.2016.31318
Clinico-biochemical and surgical evaluation of omaso-abomasal volvulus in cattle
Akraiem
A.
Department of Veterinary Medicine and Surgery, Faculty of Veterinary Medicine, Omar Al-Mukhtar University,
Libya
author
Kumper
H.
Clinic for Ruminants and Swine (Internal Medicine and Surgery), Justus-Liebig-University, Germany
author
Doll
K.
Clinic for Ruminants and Swine (Internal Medicine and Surgery), Justus-Liebig-University, Germany
author
Abd Elghany
H.
Department of Animal Medicine Faculty of Veterinary Medicine, Banha University - Egypt
author
text
article
2016
eng
The aim of the present study was to determine and characterize the clinical, hematological, biochemical parametersand outcome in cows suffering from Omaso-abomasal volvulus (OAV). Animals data were obtained from medicalrecords of 60 adult dairy cattle suffering from OAV (Clinic for Ruminants, Justus-Liebig-University, Germany).For each case, history, age, results of clinical examination, hematological and biochemical findings were recordedand analyzed. Ages of animals were between 2.3 and 8 years, (mean 4.8). Compared with the respective normalrange, there were significant increases of the heart rate, hematocrit, total bilirubin, urea blood, concentrations ofbicarbonate and base excess. Serum chloride and potassium were significantly decreased in the majority of cows.In this study, dehydration, metabolic alkalosis, hypochloremia, hypokalemia and circulatory insufficiency werethe major common findings in cows with Omasum-abomasal volvulus. Excessive stretching of the abomasal walland long lasting strangulations at the torsion-sites must be avoided (surgery should be performed as soon afterdiagnosis of AOV as possible) and correction of these conditions with aggressive intravenous fluid therapy isnecessary before the surgical intervention for correction of the hematobiochemical alterations
Benha Veterinary Medical Journal
Benha University; Faculty of Veterinary Medicine
1110-6581
30
v.
2
no.
2016
6
11
https://bvmj.journals.ekb.eg/article_31319_1d60a4ed4c23b14154b10e3ae18dbf1f.pdf
dx.doi.org/10.21608/bvmj.2016.31319
Occurrence of certain pathogens in meat meals at hospital level
Hemmat
Ibrahim
Food Control Department (Meat Hygiene), Fac. Vet. Med. Benha Univ.
author
Rasha
El-Sabagh
Animal Health Research“Benha branch"
author
Ahmed
Maarouf
Animal Health Research“Benha branch"
author
Heba
Ibraheem
Vet. At Benha Univ. Hospital
author
text
article
2016
eng
This study was conducted on 90 random samples of beef and chicken meat meals (45of each) with weight 250gmfor chicken and 150gm for beef, at kitchen hospital level represented as fresh (raw), boiled and fried samples (15of each) collected from a Governmental hospital in Kaliobia Governorate to evaluate the bacterial quality of them,and hygienic health hazard of meat meals. The bacteriological examination resulted in, seven isolates of E. coliwere isolated from examined meat samples represented as 3 from fresh chicken meat with serotypes O55:H7;O125:H18 and O78 (one from each type); 2 fried chicken meat with serotype O55:H7 and O125:H18 (one fromeach type) and 2 from Fresh beef with serotypes O55:H7 and O125:H18 (one from each type). Meanwhile, theyfailed to be isolated from boiled chicken meat; boiled beef and fried beef. In addition, 11 isolates of coagulasepositive S. aureus were isolated from examined meat samples represented as 4 (26.7%) from fresh chicken meat;2 (13.3%) from fried chicken meat; 4 (26.7%) from fresh beef and one (6.7%) from fried beef. Meanwhile, theyfailed to be isolated from boiled beef and chicken meat. Meanwhile, the present study failed to detect Salmonellaserovars from all examined meat samples
Benha Veterinary Medical Journal
Benha University; Faculty of Veterinary Medicine
1110-6581
30
v.
2
no.
2016
12
16
https://bvmj.journals.ekb.eg/article_31321_2950550b0b67d97cc603c4167db46b9b.pdf
dx.doi.org/10.21608/bvmj.2016.31321
Isolation and identification of Bovine Viral Diarrhea virus among cattle and buffaloes in Kalubeya, Egypt (2013-2014)
Saad
Sharawi
Department of Virology, Faculty of Veterinary Medicine, Benha University
author
Ayman
El-Habbaa
Department of Virology, Faculty of Veterinary Medicine, Benha University
author
Lamya
Ateya
Animal Health Research Institute, Dokki, Giza, Egypt
author
shaimaa
Abd-Elhafeez
Animal Health Research Institute, Dokki, Giza, Egypt
author
text
article
2016
eng
Bovine viral diarrhea virus (BVDV) is the most prevalent infectious disease of cattle. It causes financial losses from avariety of clinical manifestations and is the subject of a number of mitigation and eradication schemes around the world.This study was designed for isolation and identification of BVDV in Kalubeya governorate. The study was carried out on400 Buffy coat and tissue samples from cattle and buffaloes. Direct detection of BVDV antigen by antigen capture enzymelinked immunosorbent assay (AC-ELISA) showed positive results in 47.4% (95) and 10.5% (21) in cattle and 38% (76)and 6% (12) in buffaloes for buffy coat and tissue samples, respectively. Virus isolation (VI) on MDBK cell culturerevealed negative results which subjected to indirect fluorescent antibody technique (FAT), revealed characteristicintracytoplasmic apple green fluorescence indicating presence of non-cytopathogenic strain of BVDV. Moleculardetection using reverse transcription-polymerase chain reaction (RT-PCR) revealed the presence of specific PCR productat the correct expected size of the BVDV genotype I (190 bp).
Benha Veterinary Medical Journal
Benha University; Faculty of Veterinary Medicine
1110-6581
30
v.
2
no.
2016
17
22
https://bvmj.journals.ekb.eg/article_31322_09bb21365108dc9dcf41d773eef21ccc.pdf
dx.doi.org/10.21608/bvmj.2016.31322
Incidence of Staphylococcus aureus in meat products with special reference to enterotoxins
Hassan
A.
food control department, Faculty of Veterinary Medicine, Benha University
author
Mona
Ibrahim
food control department, Faculty of Veterinary Medicine, Benha University
author
Marionette
Nassif
Animal health research institute, Benha branch
author
text
article
2016
eng
The present study was designed to throw spot light upon the incidence and detection of Staphylococcal enterotoxins. Theincidence for isolation of S. aureus in the examined samples of street vended meat products was recorded in the examinedhawawshi samples at percentage of 31.4% followed by kofta samples at percentage of 25.7%. While the lowest incidencefor isolation of S.aureus in the examined samples was recorded in shawerma samples at percentage of 22.8%. Thepercentages of antimicrobial susceptibility of S. aureus isolates from the examined samples of meat products were verysusceptible to erythromycin (E) (89.3%), followed by norfloxacin (NOR) (82.1%), ampicillin (AM)(60.7%),ciprofloxacin (CP)(53.6%), streptomycin (S)(35.7%) and amoxicillin (AMX)(25.0). The study obtained "one" enterotoxinA and enterotoxin D produced by S. aureus in the Hawawshi samples of meat product. There is "one" unacceptedHawawshi sample with a percentage (3.6%). While, enterotoxins B and enterotoxin C failed to be detected in Hawawshisamples of meat product. For kofta samples there is "one" enterotoxin D produced by S. aureus. There is "one" unacceptedkofta sample with a percentage (3.6%). While, enterotoxins A, B and C failed to be detected in kofta samples of meatproducts. Also enterotoxins A, B, C and D failed to be detected in shawerma samples of meat products
Benha Veterinary Medical Journal
Benha University; Faculty of Veterinary Medicine
1110-6581
30
v.
2
no.
2016
23
27
https://bvmj.journals.ekb.eg/article_31323_805103678a8d0898af646bdfe7a89a71.pdf
dx.doi.org/10.21608/bvmj.2016.31323
Mould contamination of some Egyptian cheese
Seham
El-Badry
Educational Veterinary Hospital, Faculty of Veterinary Medicine, Zagazig University, Zagazig, Egypt
author
Amal
Raslan
Educational Veterinary Hospital, Faculty of Veterinary Medicine, Zagazig University, Zagazig, Egypt
author
text
article
2016
eng
Mould contamination of milk products is a matter of significance in the field of food safety due to its related food spoilageand production of mycotoxins. This study was undertaken to investigate the incidence of mould contamination in differentcheese types marketed in Zagazig city, Egypt. Roumy cheese had the highest total mould count followed by cheddar andwhite cheese types. The prevalent mould genera were Aspergillus, Penicillium, Cladosporium and Alternaria. Furtheridentification of Aspergillus (A) species revealed that A. niger, A. flavus, A. parasiticus and A. ochracous are the dominantAspergilli. The public health importance of the prevalent mould genera was discussed
Benha Veterinary Medical Journal
Benha University; Faculty of Veterinary Medicine
1110-6581
30
v.
2
no.
2016
28
33
https://bvmj.journals.ekb.eg/article_31325_2bbb8b0d6d199901d60a45f2b6eba4e1.pdf
dx.doi.org/10.21608/bvmj.2016.31325
Effect of Probiotics, Prebiotics, Synbiotics, Organic Acids and Enzymes Supplementation on broiler Chicks’ Immunity in relation to the Economic Performance
Liza
Mohammed
Department of Animal Wealth Development, Faculty of Veterinary Medicine, Benha University
author
Eman
Kamel
Department of Animal Wealth Development, Faculty of Veterinary Medicine, Benha University
author
Mohammed
Abo-Salem
Department of Forensic Medicine and Toxicology, Dean of Faculty of Veterinary Medicine, Benha University
author
Atallah
S.T.
Department of Forensic Medicine and Toxicology, Faculty of Veterinary Medicine, Benha University
author
El Shawarby
R.M.
Department of Animal Husbandry and
Animal Wealth Development, Faculty of Veterinary Medicine, Alexandria University
author
text
article
2016
eng
This study was conducted to evaluate the effect of different feed additives (Probiotics, Prebiotics, Synbiotics, Organicacids and Enzymes) on chicks’ immunity (Antibody titer against Newcastle vaccine, differential leukocyte count, totalproteins, albumin and globulin value) and economic efficiency analysis by using production functions under summercondition. Our results showed that, enzyme treated group recorded the highest value of white blood cells. Organic acidstreated group for Ross breed showed the highest value for lymphocyte percentage. Heterophils percentage value was thehighest for probiotics and synbiotics treated group. Concerning antibody titer to vaccination against Newcastle, we foundthat all the experimental groups had a positive effect on antibody titer. Regarding, albumin value, it was the highest forprobiotic treated group, while globulin value for Cobb breed showed higher value for all treated groups in comparison tocontrol group except for probiotic treated group. Regarding the effect of these additives on body weight and total return,we found positive relationship between feed additives and body weight and total return. On the basis of our results, itwould be concluded that probiotics, prebiotic, synbiotic, organic acids and enzymes had positive effect on immunity andeconomic performance of broiler chicken.
Benha Veterinary Medical Journal
Benha University; Faculty of Veterinary Medicine
1110-6581
30
v.
2
no.
2016
34
44
https://bvmj.journals.ekb.eg/article_31327_0e22038dfbd0c688e52d24acdcee4284.pdf
dx.doi.org/10.21608/bvmj.2016.31327
Histological study on the vesicular glands of castrated and non-castrated bucks
Mahmoud
Emam
Histology and Cytology Dept., Faculty of Veterinary Medicine, Benha University
author
Ehab
El-Zoghby
Histology and Cytology Dept., Faculty of Veterinary Medicine, Benha University
author
Badia
Abughrien
Anatomy Dept., Faculty of Veterinary
Medicine, Tripoli University
author
text
article
2016
eng
The present study was investigated the histological structure of the vesicular glands of eight mature castrated and noncastratedzaraibi bucks (4 animals/ each group). Generally, the vesicular gland of bucks was compact and lobulated gland.It was covered by a muscular capsule sending fibro-muscular septa dividing the gland into irregular unequal lobules. Eachlobule was formed of numerous secretory acini and few amount of inter-acinar connective tissue stroma. Each lobule hada central collecting sinus to collect the secretions from the different acini. Both secretory acini and central collectingsinuses were lined mainly with pseudostratified columnar epithelium that was consisted mainly of secretory columnarcells and few small basal cells. The secretion was released into the acinar limen via apocrine mode. The castrated bucksshowed more connective tissue and less acinar components in comparison to non-castrated bucks. Such finding representsthe histological difference between the vesicular gland of castrated and non-castrated bucks that pointed out the effect ofcastration on structure of the vesicular gland
Benha Veterinary Medical Journal
Benha University; Faculty of Veterinary Medicine
1110-6581
30
v.
2
no.
2016
45
50
https://bvmj.journals.ekb.eg/article_31328_cc370dacc374bf248af81b8db5fceb46.pdf
dx.doi.org/10.21608/bvmj.2016.31328
Prevalence of hard tick infesting cattle with a special reference to microscopic and molecular early diagnosis of tick born piroplasms
Ramadan
M.Y.
Parasitology Department, Faculty of Veterinary Medicine, Benha University, Moshtohor, Toukh,13736, Egypt
author
Elakabawy
L.M.
Parasitology Department, Faculty of Veterinary Medicine, Benha University, Moshtohor, Toukh,13736, Egypt
author
Elmadawy
R.S.
Parasitology Department, Faculty of Veterinary Medicine, Benha University, Moshtohor, Toukh,13736, Egypt
author
Kamal
MM
Parasitology Department, Faculty of Veterinary Medicine, Benha University, Moshtohor, Toukh,13736, Egypt
author
text
article
2016
eng
The current study aimed to conclude the prevalence of hard ticks infesting cattle in Qalyobia governorate and to investigatethe developmental stages of cattle piroplasms in ticks hemolymph. A total of 600 cattle were haphazardly examined for tickinfestation throughout the period from April 2013 to March 2014. Hemolymph samples of 1550 ticks were extracted, stainedby Giemsa again and microscopically inspected under oil immersion lens. Moreover, PCR assay was applied to amplify18SrRNA genes of Babesia and Theileria in 40 microscopically negative hemolymph samples. The study revealed that6.17% of cattle were infested by ticks. Rhipicephalus turanicus and Rhipicephalus praetextatus (70.02% and 29.98%respectively) were the recorded tick species. Ticks showed a great tendency to attach to the udder and inguinal regions(34.46%), while the two sides were the least infested (7.69%). No recorded significant effect of sex on the rate of infestation(P> 0.05). Cattle at age of 3-5 years were significantly more infested (78.38%) than those of 2-3 years (16.22 %) and thoseof 8months -2 years (5.41%). Microscopic examination of tick hemolymph revealed the presence of piroplasmdevelopmental stages in 39.23% and 49.4% of hemolymph samples obtained from Rhipicephalus turanicus andRhipicephalus praetextatus respectively. PCR analysis further revealed that amongst 40 ticks whose hemolymph weremicroscopically negative, 25% and 12.5% were positive for Babesia and Theileria sp. respectively. The results concludedthat Rhipecephalus turanicus and Rhipicephalus praetextatus are the common tick species infesting cattle in the study area.PCR assay is proved to be more efficient and sensitive than the microscopic assay for identification of the developmentalstages of piroplasms in tick hemolymph. Thus, allowing the easy and rapid surveillance of the endemicity of piroplasminfection among tick sp. and consequently application of emphasized control programs
Benha Veterinary Medical Journal
Benha University; Faculty of Veterinary Medicine
1110-6581
30
v.
2
no.
2016
51
60
https://bvmj.journals.ekb.eg/article_31329_b23c41b3bec8c4c8a3a14c899c2d259c.pdf
dx.doi.org/10.21608/bvmj.2016.31329
Clinical, hematological and biochemical changes in lambs suffered from nutritional muscular dystrophy
Ghanem
M.
Animal Medicine Dept., Fac. Vet. Med., Benha University
author
Anwar
R.
Animal Medicine Dept., Fac. Vet. Med., Benha University
author
Abd El-Raof
M.
Animal Medicine Dept., Fac. Vet. Med., Benha University
author
El-Attar
M.
Animal Medicine Dept., Fac. Vet. Med., Benha University
author
text
article
2016
eng
Nutritional Muscular Dystrophy (NMD) is a disease of lambs results from a selenium and/or vitamin E deficiency. Thisstudy was applied on 20 NMD lambs and 20 control native breed lambs from Qalyubia Governorate, with ages rangedfrom 1 to 4 months, in order to explore the effects of NMD clinical and hematobiochemical changes in lambs. Affectedlambs showed sternal recumbency, stiffness, trembling and muscle tremors, hard swollen muscle and the lamb adoptedlateral recumbency. The diseased lambs had significant (P<0.05) decreases in the serum Se and vitamin E. The RBCscount, Hb content and PCV% were significantly decreased (P<0.05) in NMD lambs. On other hand, the MCV and MCHwere significantly increased (P<0.05) in NMD lamb compared to control. The activities of ALT, AST, CPK, LDH andMDA were significantly increased (P<0.05) in NMD lamb compared to control group. The SOD and GSH-Px weresignificantly decreased (P<0.05) in NMD lamb compared to control. It was concluded that vitamin E and Se deficiencyresult in NMD which associated with alteration in clinical and hematological parameters
Benha Veterinary Medical Journal
Benha University; Faculty of Veterinary Medicine
1110-6581
30
v.
2
no.
2016
61
66
https://bvmj.journals.ekb.eg/article_31331_91995c9f0fe390d5372d4f5aa3f71a7b.pdf
dx.doi.org/10.21608/bvmj.2016.31331