The present study aimed to assess the lipid peroxidation and antioxidant enzymes activities of diluted rabbit semenenriched with different concentrations of pomegranate peel methanolic extract (PPME) during chilled storage. Semenwas collected from 10 rabbit bucks, pooled, then divided into five aliquots and diluted each in 5 ml Tris-citric acidglucose-egg yolk extender (TCGY). The 1st aliquot served as control, while PPME was added in different concentrations(1.6, 2.0, 2.4 and 2.8 mg/5 ml TCGY extender) to the aliquots 2, 3, 4 and 5, respectively. Diluted semen samples wererefrigerated at 5°C for 72 hours. Lipid peroxidation (MDA), H2O2 concentration, SOD, CAT activities and GSHconcentration were evaluated in chilled seminal plasma during the chilling period. Obtained results clearly demonstratedthat the addition of 1.6 – 2.8 mg PPME in the chilled seminal plasma proved to be beneficial for minimizing MDA andH2O2 concentrations; in addition, they increased SOD, CAT activities and GSH concentration compared to the control.While, the concentrations of 2.4 and 2.8 mg were the best PPME enrichments that maintained the highest SOD, CATactivities and GSH concentration. It was concluded that the enrichment of rabbit semen TCGY extender with 2.4 – 2.8mg PPME during chilling had significantly decreased sperm lipid peroxidation and H2O2 production beside they enhancedthe activities of antioxidant enzymes and the reduced glutathione.