@article { author = {Abo-Sakaya, Rania and Bazan, Naglaa}, title = {Molecular detection of novel bovine Ephemeral fever Virus strain and its effect on immune system in cattle, Egypt 2017}, journal = {Benha Veterinary Medical Journal}, volume = {38}, number = {2}, pages = {1-4}, year = {2020}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2020.26385.1193}, abstract = {BEF is an immune related acute febrile disease affecting cattle characterized by expression of inflammatory mediators. This study aimed to clarify immune and inflammatory changes that result from BEF infection in cattle particularly cytokines mechanisms. The study was performed on 100 cattle, 2 - 3 years of age, from endemic areas with three-day sickness in Qaliubiya governorate, Egypt. Characteristic clinical signs appeared on cattle from suspected farms varied from transient fluctuating fever, stiffness, lameness, enlargement of peripheral lymph nodes, decreased milk yield and muscular shivering. So, we performed RT-PCR to confirm the cause of disease by direct detection of the virus targeting glycoprotein coding (G) gene , we found 30 cases positive BEF out of 100 animals. A negative control group was 10 healthy cows that was compared with positive infected group to show effect of virus on immune system by evaluating parameters change by infection, included endogenous pyrogenic variables such as cytokines, Interleukins (IL-2 and IL-6) and inflammatory biomarkers as (cortisone, CRP). Our results demonstrated substantial increase (P<0.05) in serum concentrations of IL-2, IL-6, cortisone and CRP as well as hypocalcemia in infected animals.}, keywords = {Bovine ephemeral fever,PCR,Interleukins,inflammatory biomarkers,cattle}, url = {https://bvmj.journals.ekb.eg/article_111417.html}, eprint = {https://bvmj.journals.ekb.eg/article_111417_6b0b935c148ad5259ed70e799d46926c.pdf} } @article { author = {lotfy, asmaa and Selim, Abdelfattah and Ibrahim, Abdel Moneim and salem, Sayed}, title = {Seroprevalence and Molecular characterization of Bovine viral diarrhea, Rota and corona viruses in neonatal cattle and buffalo calves in some governorates in Egypt}, journal = {Benha Veterinary Medical Journal}, volume = {38}, number = {2}, pages = {5-9}, year = {2020}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2020.24852.1171}, abstract = {The present study aims to detect the prevalence and molecular characterization of Bovine viral diarrhea, Rota and corona viral diseases as major causes of diarrhea that mainly leads to neonatal deaths among neonatal calves in some governorates in Egypt (Kafr El Sheikh, El Menofyia, Sharkia, Bahaira and El-Gharbia). The infection rate of the three examined viral diseases uses showed non-significant difference between sex and age of cow calves, and the infection more common on female younger calves. Also the infection rate in female young buffalo calves. Selective positive samples undergo Sequencing and compared with other strains in GenBank. The results showed that the Egyptian BVD strain is closely related to BVD strain from Argentina (DQ088995) and Colombia (MH198305), while Bovine Rota (BRV) showed high similarity with Bovine Rota strain of South Korea and Turkey but Bovine Corona virus (BCoV) was closed to Bovine Corona viruses (BCoV) strain isolated from France .}, keywords = {BVD,BRV,BCV,Sequencing,phylogenetic}, url = {https://bvmj.journals.ekb.eg/article_111834.html}, eprint = {https://bvmj.journals.ekb.eg/article_111834_3b57e768b9f8e63645ee6a86bdf077ae.pdf} } @article { author = {Helal, Mahmoud}, title = {The relationship between deficiency of some trace elements, oxidative stress, immunoglobulin E and vitamin A in sheep affected with skin diseases}, journal = {Benha Veterinary Medical Journal}, volume = {38}, number = {2}, pages = {10-16}, year = {2020}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2020.24929.1174}, abstract = {This work aimed mainly to study the status of some trace elements, oxidative stress, immunoglobulin E and vitamin A in sheep with skin diseases. In this study a survey was done on 190 of sheep were taken from sheep flocks around Menoufia Governorate showing skin diseases of both sexes of 2 ± 0.5 y of age. All cases were subjected to clinical examination in which body temperature was taken also pulse rate, respiratory rate; rumen movements, mucous membrane, wool and body condition score. 58% of these animals suffering from infectious causes. The other 42% of animals was associated with nutritional deficiencies. A significant decrease (p˂0.01) was found in the level of zinc, copper and vitamin A than that taken from apparently healthy animals. Also, there was significant decrease (p˂0.05) in alkaline phosphatase activity. However, a significant increase (p˂0.05) was found in catalase activity. A significant decrease (p˂0.01) was found in superoxide dismutase activity compared to apparently healthy animals. We found significant increase (p˂0.05) in Malondialdehyde level than that taken from apparently healthy animals. A significant increase (p˂0.05) was detected in IgE level compared to apparently healthy animals. According to the results of our study we concluded that the prevalence of non-infectious skin affections was (42%) and there was significant relationship between non-infectious skin affections and oxidative stress, some trace elements, vitamins, immunoglobulin levels in sheep under field conditions.}, keywords = {sheep,Skin diseases,nutritional,Oxidative Stress}, url = {https://bvmj.journals.ekb.eg/article_111835.html}, eprint = {https://bvmj.journals.ekb.eg/article_111835_65b55311f56abc21b01fd3559ea2549c.pdf} } @article { author = {Emam, Rania and Ghanem, Mohamed and Abdel-Raoof, Yassen and Elkhyat, Heba}, title = {Evaluation of some hepatoprotective preparations in experimentally induced hepatopathy in Baladi goats}, journal = {Benha Veterinary Medical Journal}, volume = {38}, number = {2}, pages = {17-25}, year = {2020}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2020.26506.1194}, abstract = {This work aimed to evaluate the hepatoprotective effect of garlic oil extract and artichoke extract with regarding to clinical, hematobiochemical alteration and oxidative stress status of induced acute hepatopathy in Baladi goats. Fifteen Baladi goats were kept for experimental induction of hepatopathy that treated orally with 0.3 ml /kg of carbon tetrachloride (CCL4)once and divided randomly into 3 groups each of five. Group A was kept as control positive. Group B and Group C were treated by garlic oil extract in dose 0.8mg/kg and artichoke extract in dose 10mg/kg for 15 days for hepatic protection before induction. Clinical and hematobiochemical examination were carried out at 1st, 3rd and 7th day after induction. Clinical examinations of CCl4 treated group at first day showed dullness, inappetence, pale mucous membranes, elevated body temperature and pulse rate and nervous manifestations. While garlic extract and artichoke extract l treated groups showed less severe clinical signs that began to disappear at 3rd day after induction. Hematobiochemical examination: induced acute hepatopathy group showed significant (P<0.05) decrease in RBCs, Hb, PCV, GPX and SOD and significant (P<0.05) increase in WBCs, ALT, AST, GGT, total and direct bilirubin, blood urea nitrogen, creatinine and MDA. While garlic extract and artichoke extract treated groups showed significant (P<0.05) increase in RBCs, Hb, PCV and antioxidant status with significant (P<0.05) decrease in liver damage indices of treated animals. Therefore, we concluded that garlic oil extract and artichoke extract could be used as hepatoprotective agent in Baladi goats.}, keywords = {induced hepatopathy,garlic oil,artichok extract,Baladi goats}, url = {https://bvmj.journals.ekb.eg/article_111836.html}, eprint = {https://bvmj.journals.ekb.eg/article_111836_4a6a085eaab91f350acfd52a4050a562.pdf} } @article { author = {Mohamed, Abdelmonem}, title = {Dietary Abomasal Impaction In Weaned Egyptian Calves}, journal = {Benha Veterinary Medical Journal}, volume = {38}, number = {2}, pages = {26-29}, year = {2020}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2020.26559.1195}, abstract = {Abomasal disorders can lead to severe and potentially life-threatening conditions for the affected calves. In this study, the dietary abomasal impaction was investigated in 12 weaned buffalo and cow calves aged 6-12 months admitted to the Veterinary Teaching Hospital, Faculty of Veterinary Medicine, Zagazig University, Egypt, five apparently healthy cow calves were also included as controls. The diagnosis was based upon case history, general clinical examination with special reference to the gastrointestinal system, and the abomasal measurements including length, width, and depth that taken for all cases under ultrasonographic guidance. Non-significant differences were found for the measured physical parameters in the clinical cases versus controls. Right ventral abdominal distension was reported in all cases, evacuation of dark greenish ruminal fluid using the stomach tube was reported also and to a lesser extent the oral feed regurgitation. Significant differences were found in all the abomasal dimensions in cases when compared to control (P<0.0001). Also, the ultrasonographic examination revealed the extension of the abomasum beyond its limits. The abdominal ultrasound as a quick, feasible and non-invasive tool showed relative accuracy in the diagnosis of abomasal impaction in weaned calves.}, keywords = {Abomasum,Ultrasound,Dimensions,dietary,distension}, url = {https://bvmj.journals.ekb.eg/article_111840.html}, eprint = {https://bvmj.journals.ekb.eg/article_111840_db43e01ebd3104237f598dade658ec6d.pdf} } @article { author = {Abdelrahman, Samar and Shams, Gamal and Abd El-Latif, Suhair}, title = {Effect of Moxifloxacin and Role of Vitamin (E) on Antioxidant Enzymes Activity in Rats}, journal = {Benha Veterinary Medical Journal}, volume = {38}, number = {2}, pages = {30-33}, year = {2020}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2020.19409.1132}, abstract = {Our investigation was carried out to assess the protective impact beside the antioxidant role of vitamin E on Moxifloxacin's possible adverse effects. Collection of blood samples at 1st, 7th, 14th, 21st days after treatment. Collection of tissue samples at 7th and 14th days after treatment to evaluate the protective effect of Vit. E. Our results showed (day 14moxifloxacin): multifocal hepatic necrosis of variable sizes, partially replaced by macrophages occasionally with giant cells formation. The hepatic blood vessels were moderately congested and the bile ducts were proliferated with characteristic portal round cells aggregation and portal fibrosis. Examined sections from kidney showed cystic dilatation of few tubules in the medulla and cortex. The renal pelvis revealed focal sloughing and hyperplasic changes in the transitional epithelium. Focal interstitial and perivascular aggregation of round cells and eosinophils were observed. The renal blood vessels were mildly congested. Day 14 moxifloxacin and Vit. E: sections showed normal hepatic parenchyma with residual portal biliary proliferation and fibrosis. Minute focal hepatic necrotic areas partially replaced by round cells were also seen. Also showing apparently normal nephron units with mild degenerative changes in some tubular epithelium and cyst dilatation in some renal tubules. Therefore, Vitamin E should be taken with moxifloxacin to decrease its adverse effects.}, keywords = {Moxifloxacin,Vitamin E,Antioxidant activity,histopathology}, url = {https://bvmj.journals.ekb.eg/article_111842.html}, eprint = {https://bvmj.journals.ekb.eg/article_111842_593940e40ae251d874bb1d2685078565.pdf} } @article { author = {Eladawy, Samar and El-Bagoury, Gabr and El-Habbaa, ayman and El-Mahdy, sausan}, title = {Evaluation of formaldehyde and binary ethylenimine inactivated Newcastle Disease Virus Vaccine from new isolate compared with imported NDV vaccine}, journal = {Benha Veterinary Medical Journal}, volume = {38}, number = {2}, pages = {34-40}, year = {2020}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2020.21635.1148}, abstract = {Even massive vaccination programs, the incidence of Newcastle Disease virus (NDV) outbreaks in Egypt is still frequent with appearing new isolates that need production of new vaccines. In this study, NDV vaccine was prepared by using a new local isolate (NDV/Ch/Giza2014) inactivated by two different chemicals binary ethylenimine (BEI) and formaldehyde. The formaldehyde inactivated NDV (NDVF), BEI inactivated NDV (NDVEI) and imported inactivated Newcastle Disease virus (NDVI) vaccines were examined for generating humoral immune response in different groups of specific pathogen free (SPF) chicks. Vaccinated Chicks by NDVEI vaccine gave higher serum antibody titers than NDVF and NDVI Vaccines using Haemagglutination Inhibition (HI) test. The prepared and imported vaccines gave near percentage of protection against the local and the classical strain in chicks. It could be concluded that the locally prepared inactivated NDV vaccines can protect chicken against either homologous or heterologous challenging viruses and the NDVEI Vaccine gave higher protection percentage than NDVF and NDVI Vaccines.}, keywords = {NDV,inactivated vaccine,binary ethylenimine,Formaldehyde}, url = {https://bvmj.journals.ekb.eg/article_111843.html}, eprint = {https://bvmj.journals.ekb.eg/article_111843_b41fd961641a924a54fd33862fec07c3.pdf} } @article { author = {Eladawy, Samar and El-Bagoury, Gabr and El-Habbaa, ayman and El-Mahdy, sausan}, title = {Efficacy of an inactivated Vaccine prepared from a new isolate of Newcastle Disease Virus}, journal = {Benha Veterinary Medical Journal}, volume = {38}, number = {2}, pages = {41-46}, year = {2020}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2020.21636.1149}, abstract = {The incidence of Newcastle Disease virus (NDV) outbreaks in Egypt is still frequent even massive vaccination programs that due to viral mutation and appearing new isolates that need new vaccination program. Inactivated Newcastle Disease virus (NDV) vaccine was prepared using a new local isolate (NDV/Ch/Giza2014), from Giza governorate, Egypt, inactivated with binary ethylenimine (BEI) and emulsified with Montanide ISA70VG as an oil adjuvant. The prepared vaccine was evaluated in comparison with an inactivated imported vaccine. Chick groups vaccinated with either prepared or imported vaccines showed high serum antibody titers from the third week post vaccination until reached the maximum titer at the 9th week post vaccination using Haemagglutination Inhibition (HI) test. The prepared and imported vaccines gave 95-100% of protection against the local and the classical strain in chicks, during challenge period, there no clinical signs or lesions on examination. Our Conclusion that the locally prepared inactivated NDV vaccine can protect chicken against either homologous or heterologous challenging viruses.}, keywords = {ND,Evaluation,inactivated vaccine,binary ethylenimine}, url = {https://bvmj.journals.ekb.eg/article_111845.html}, eprint = {https://bvmj.journals.ekb.eg/article_111845_4711d0850ab846a6e25bfb1bc8bb6a1a.pdf} } @article { author = {Amin, Aziza and Amin, Aziza}, title = {The Effect of the Hydroxyapatite and Hydroxyapatite Doped by Magnesium on Healing of Fracture in Dogs}, journal = {Benha Veterinary Medical Journal}, volume = {38}, number = {2}, pages = {47-51}, year = {2020}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2020.28949.1202}, abstract = {The aim of this study was to investigate and compare the effect of the Nano Hydroxyapatite (NHA) and Nano Hydroxyapatite doped by magnesium (NHA-Mg) on healing of fracture in dogs. Twenty-seven adult dogs of both sexes aging between 2-3years old were randomly assigned into three groups. Group one (control group), group two (NHA group), and group three (NHA-Mg). An experimental model of diaphyseal complete radial fracture was surgically created by gegli saw. The fractured radius was fixed by extra-medullary 6-hole dynamic compression plate (6 hole DCP), with addition of the previously mentioned materials to fill the radial fracture defect in the corresponding groups. Data of clinical observation, plain radiography, computed tomography (CT) and histopathological examination and scoring were recorded on 45, 75, 90 days after induction of fracture. The dogs of all groups achieved the full bearing weight and stance 5 days postoperatively. The radiographs admit little knowledge about the healing scoring rather than the CT which declares that N HA-Mg was improve the filling of fracture defect. The histopathological examination and scoring revealed that NHA-Mg was superior HA alone in fracture healing. Data of the present work showed that nano hydroxyapatite doped by (0.007mol) magnesium concentration allows complete calcification and healing of the fractured radius during the experimental time frame.}, keywords = {Nano hydroxyapatite,fractured bone,Healing,dogs,histopathology}, url = {https://bvmj.journals.ekb.eg/article_111846.html}, eprint = {https://bvmj.journals.ekb.eg/article_111846_6e61c5cd523fadbf770106b2d2f8a2c4.pdf} } @article { author = {fararh, khalid and El-Shemy, Mona and Moussa, Ghada}, title = {Hepatoprotective effect of rutin and N-acetyl cysteine against isoniazid induced hepatotoxicity}, journal = {Benha Veterinary Medical Journal}, volume = {38}, number = {2}, pages = {52-56}, year = {2020}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2020.30988.1210}, abstract = {This study was carried to evaluate the protective effect of rutin and N-acetyl cysteine (NAC) against isoniazid-induced (INH) hepatotoxicity. In this study, Seventy two adult male rats were divided into six equal groups as follow: control group: 1 ml saline /Kg b.wt I/P. INH group: 100 mg INH /kg b.wt I/P. INH + Rutin group: 100 mg INH /kg b.wt I/P with 200 mg rutin /kg orally. INH + NAC group: 100 mg INH /kg b.wt I/P with 300 mg NAC /kg orally. Rutin group: 200 mg rutin /kg/day orally. NAC group: 300 mg NAC /kg b.wt orally. Serum and tissue specimens were collected at 7th, 14th and 21th day. IP injection of Isoniazid induced significant increase in values of ALT, AST, ALP, GGT and total bilirubin, while significant decrease in total protein and albumin was recorded. INH + Rutin showed significant decreases in the levels of ALT, AST, ALP and GGT with significant increase in albumin values and significant increase in total protein however significant decrease in the level of total bilirubin was recorded only at 21 days. INH + NAC induced significant decreases in the levels of ALT, AST, ALP and GGT with significant increase in albumin and total protein at 14 and 21 days and significant decrease in total bilirubin at 21 days. Rutin and NAC have potent protective effect against INH induced hepatotoxicity.}, keywords = {Isoniazid,Rutin,N-acetyl cysteine,Hepatotoxicity}, url = {https://bvmj.journals.ekb.eg/article_111848.html}, eprint = {https://bvmj.journals.ekb.eg/article_111848_4726714396e47b7b93c31bbff327d187.pdf} } @article { author = {ghania, asmaa and Sosa, G. and Mahmoud,, A. and Agag, M}, title = {Testicular biometry, ultrastructure, cellular cytometry and hormonal profile in relation to age in Egyptian buffaloes.}, journal = {Benha Veterinary Medical Journal}, volume = {38}, number = {2}, pages = {57-64}, year = {2020}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2020.30803.1209}, abstract = {This study was conducted on a number of (30) buffaloes in Qalubia. The age ranged from 12 to 36 month. There were divided into two groups: group (I) and group (II), their ages ranged from}, keywords = {testis,Buffalo bull,hormonal profile,Flowcytometry,Electron Microscope}, url = {https://bvmj.journals.ekb.eg/article_131774.html}, eprint = {https://bvmj.journals.ekb.eg/article_131774_853fd4d38322a0d3064b879157e85c30.pdf} } @article { author = {Khalil beltagy, Rana and Saad, Saad and Hassanien, Fatin and Mousa, Mohamed}, title = {Monitoring of some Pathogenic bacteria in Nile fish}, journal = {Benha Veterinary Medical Journal}, volume = {38}, number = {2}, pages = {65-70}, year = {2020}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2020.24799.1169}, abstract = {The present study was designed to monitor the incidence of pathogenic bacteria in Nile fish. A grand total of ninty random samples of fresh Nile fish Oreochromis Niloticus (Nile tilapia) , Clarias Gariepinus (African catfish) and Cyprinus Carpio (Common carp) (30 samples of each) were randomly and periodically collected from different markets in El Gharbia governorate, Egypt, then packaged and marked individually in polyethylene bags. These collected samples were bacteriologically and serologically examined. we fond that the Incidence of E .coli isolated from the examined fish samples were (21)70%.(10)33.3% and(22073.3% from Nile tilapia,African catfish and Common carp respectively, they were serotype as O84, O26 O128, O17 and O119. S.areas was (8)26.6%,(18)60% and(20)66.6%from the examined Nile tilapia,African catfish and Common carp.Salmonella failed to be detected in any of the examined fish samples. Aeromonas was isolated from(24)80%,(30)100% and (28)93.3%.while Psedomonas was (28)93.35,(30)100% and(16)53.3%from the examined Nile tilapia,African catfish and Common carp respectively.It was conclouded that Nile fish contaminated with many food poisoning bacteria.}, keywords = {Nile Fish,Pathogenic bacteria,E.coli,Salmonella. Aeromonas}, url = {https://bvmj.journals.ekb.eg/article_112168.html}, eprint = {https://bvmj.journals.ekb.eg/article_112168_baae169aab280a0a512a139d40d35e8e.pdf} } @article { author = {Khalil beltagy, Rana and Saad, Saad and Hassanin, Fatin and mosa, mohamed}, title = {Effect of Pomegranate and Moringa Extract on E. coli load in Nile tilapia.}, journal = {Benha Veterinary Medical Journal}, volume = {38}, number = {2}, pages = {71-75}, year = {2020}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2020.24803.1170}, abstract = {Pomegranate and Moringa oleifera are useful food of great interest and they have multiple useful effects on human health. Pomegranate and Moringa oleifera were used to increase the nutritional and hygienic quality of food by using their extracts. The antibacterial action of ethanolic extract of (1% pomegranate, 1% Moringa oleifera and 1% mixture from pomegranate and Moringa oleifera) were examined against E.coli (O128:H2) artificially inoculated into fresh tilapia fish and stored at 4 ± 1°C then were analyzed for sensory evaluation and E. coli count. The results showed that the sensory analysis of all treatments and control fish were acceptable for all judgment members either fresh or during storage. The combinations of 1% pomegranate peels and 1% mohringa had the highest inhibitory effect against E.coli population (p < 0.05) during the storage periods. It was concluded that the ethanolic extracts of both pomegranate peels and Moringa oleifera leaves can be used for fish preservation to improve its quality and increase its shelf life.}, keywords = {pomegranate peels,Moringa oleifera,Tilapia fish,E. coli}, url = {https://bvmj.journals.ekb.eg/article_111750.html}, eprint = {https://bvmj.journals.ekb.eg/article_111750_ab5cfd95dccc496c5412875d7a91e9f3.pdf} } @article { author = {Edris, Ehsan and Shaltout, Fahim and Lamada, Hanan}, title = {bacteriological examination of some ready to eat meat and chicken meals}, journal = {Benha Veterinary Medical Journal}, volume = {38}, number = {2}, pages = {76-79}, year = {2020}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2020.25529.1182}, abstract = {Sixty random samples of ready to eat chicken and meat meals including meat, chicken, beef kofta and chicken kofta (15 of each) were collected from different restaurants from Tanta city to evaluate their bacteriological quality The mean values of Areobic plate count(APC) , Enterobacteriacae, coliform counts(CFU/g) were 6.03×103 ± 1.45, 3.16×103 ± 0.72,7.43×102 ± 1.05 for meat,8.58×103 ± 1.65,6.53×103 ± 1.24,9.18×102 ± 2.07 for chicken , 9.91×103 ± 2.18, 5.25×103 ± 0.86, 1.06×103 ± 0.19 for beef kofta and 2.03×104 ± 0.43, 9.14×103 ± 2.06,3.32×103 ± 0.45 for chicken kofta, respectively. The results showed that 12 isolates of E.coli were identified from the examined ready to eat chicken and meat meals with different percentages as follow O26: H11 EHEC (6.67%)& O111: H4 EHEC (6.67%) for meat, O26: H11 EHEC (13.33%)& O124 EIEC (6.67%) for beef kofta, O78 EPEC(6.67%)&O127:H6 ETEC(6.67%)&O146:H21EPEC(6.67%) for chicken and O26:H11EHEC(13.33%)&O91:H21EHEC(6.67%)&O121:H7 EHEC(6.67%) for chicken kofta. Also there are 6 isolates of salmonella from the examined meals were identified. Also, there are 21 S.aureus from examined samples represented as 20% from meat, 40% from beef kofta, 33.33% from chicken and 46.67% from chicken kofta. Thus the current results in this study allowing concluding that all examined samples were contaminated with different bacteria as E.coli, Salmonella and S.aureus. And the highest APC was in chicken kofta followed with beef kofta, chicken and meat.}, keywords = {Meat and chicken meals,APC,coliforms,E.coli,Salmonella}, url = {https://bvmj.journals.ekb.eg/article_112171.html}, eprint = {https://bvmj.journals.ekb.eg/article_112171_f2b256759131aa89e42968d85d2ca23e.pdf} } @article { author = {abdelhameed, bashayer and Salem, Amany}, title = {safety of meat meals served at a university hostel}, journal = {Benha Veterinary Medical Journal}, volume = {38}, number = {2}, pages = {80-83}, year = {2020}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2020.29202.1203}, abstract = {This study aimed to evaluate the hygienic status of served chicken meat and beef in a university hostel. About one handred random of chicken and beef meat samples before and after cooking (about 120g) were collected from a university student hostel, Egypt. .Samples were examined bacteriologically, The average values (cfu/g) of APC, Enterobacteriacae & Coliform counts were 4.10×107± 0.01×107 , 4×102±0.03×102& Less than 10 in raw chicken thigh , 2.47×107 ±0.02×106 , 6×102±0.02×102& 4.3×102±0.01×102 in raw chicken breast , 2.4×103±0.03×103, Less than 10& Less than 10 in cooked chicken thigh, 5.3×104±0.02×103, Less than 10&Less than 10 in cooked chicken breast , 4×107 ±0.02×107, 1×103±0.03×103&1.3×104±0.01×103 in raw beef and 4×103±0.03×102, 2.2×10±0.02×10& Less than 10 in cooked beef, respectively. Moreover, the incidence of E.coli was 73.33%, 33.33% and 35% in raw chicken thigh, cooked chicken breast and raw beef., while the mean valued of S.aureus were 5.3×103±0.02×103, 2.3×103±0.01×102, 6.2×10±0.02×10, 6.9×10±0.02×10, 3.9×104±.01×103and less than 10 in raw chicken thigh, raw chicken breast, cooked chicken thigh, chicken chicken breast, raw beef and cooked beef, respectively. All samples were accepted based on their APC, Enterobacteriacae, Coliform & S.aureus counts. .}, keywords = {APC,enterobacteriacae,Coliform,S. aureus,E.coli}, url = {https://bvmj.journals.ekb.eg/article_112177.html}, eprint = {https://bvmj.journals.ekb.eg/article_112177_0e19529bfb62df70ea5cd595d2b4d5a2.pdf} } @article { author = {abdelhameed, bashayer and salem, amani}, title = {Antibacterial efficiency of apple cider vinegar and pomegranate molasses on meat meals served at a university student hostel}, journal = {Benha Veterinary Medical Journal}, volume = {38}, number = {2}, pages = {84-87}, year = {2020}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2020.29207.1204}, abstract = {Antibacterial effect of pomegranate molasses (PM) and apple cider vinegar (ACV) on chicken meat and beef samples before and after cooking was investigated in this study. The samples were separated into six groups as control (untreated), and treated ones with 5%PM, 3% PM, 4% ACV, 2% ACV and mix between two concentrations (2% ACV & 3%PM). A total of 24 samples of chicken and meat were collected from a university hostel. The samples were marinated by the previous concentrations of PM and ACV for 2 hours. Half of them were cooked, then each raw and cooked samples were kept in a separate plastic bag and transferred directly to the laboratory in an insulating refrigerated container under complete aseptic conditions (the experiment was repeated three times).The samples were examined to determine APC, Enterobacteriacae, coliform and the incidence of Salmonella, E. coli and S. aureus. The best results were obtained by the treated groups by the mix of 2% ACV& 3%PM and 5% PM as compared with the other treated groups.}, keywords = {Pomegranate Molasse,apple cider vinegar,Staph. aureus and E.coli}, url = {https://bvmj.journals.ekb.eg/article_112178.html}, eprint = {https://bvmj.journals.ekb.eg/article_112178_9e65431ff03c97c9ff763e60795c217a.pdf} } @article { author = {sobhy, Asmaa and Shaltout, Fahim}, title = {Prevalence of some food poisoning bacteria in semi cooked chicken meat products at Kaliobyia governorate with using of recent Vitek 2 compact and PCR techniques}, journal = {Benha Veterinary Medical Journal}, volume = {38}, number = {2}, pages = {88-92}, year = {2020}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2020.25545.1183}, abstract = {Bacterial food borne pathogens are important food safety issue worldwide .Rapid and accurate identification of bacteriological pathogen isolated from food staff is important both for food quality assurance and for the tracing of outbreaks of bacterial pathogen.A grand total of ninety random samples of semi cooked chicken meat products represented by strips, pane and nuggets (30 of each)were collected from different supermarkets at Kaliobyia governorate for bacteriological examination using conventional culture method and rapid modern techniques as Vitek 2 compact system and molecular identification by PCR technique. The obtained results revealed that Staph aureus and E.coli incidence were 20% and 10% in strips, 26.6%and 13.3% in chicken pane and26.6% and 23.3%in nuggets for total examined samples.Also,the serologically identified E.coli were O127, O128, O153, O157 and O91 from examined samples. . Staphylococcus aureus and E. coli were identified in all examined samples by confirmatory identification using Vitek 2 compact system in examined 10 random samples of the chicken products. Result recorded accuracy 100% for examined samples and showed that the Vitek 2 system is a suitable tool for rapid and direct identification of gram positive cocci and gram negative bacilli from chicken products. Using of PCR technique revealed that there were one or more virulence genes in E.coli (stx1&stx2) strains isolated from the examined samples of chicken products.}, keywords = {Staph.aureus,E.coli,VITEK 2,PCR}, url = {https://bvmj.journals.ekb.eg/article_112172.html}, eprint = {https://bvmj.journals.ekb.eg/article_112172_edc8594dfa3072b5f2719e4b79210f83.pdf} } @article { author = {sobhy, Asmaa and Shaltout, Fahim}, title = {Detection of some food poisoning bacteria in some semi cooked chicken meat products marketed at Kaliobyia governorate}, journal = {Benha Veterinary Medical Journal}, volume = {38}, number = {2}, pages = {93-96}, year = {2020}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2020.25547.1184}, abstract = {Ninety random samples of semi cooked chicken meat products represented by strip, pane and nuggets (30 of each) were collected from different supermarkets at Kaliobyia governorate for bacteriological examination.The mean values of Aerobic plate count,Enterobacteriacea count and Staphylococcus count were4.9x104 ± 1.2x104, 3.1x102 ± 8.2x102 and1.5x102 ±7.1x10 for chicken strips, 8.4x104± 3.3x104,8.9x102 ± 5.6x102and7x103 ± 4.5x103 cfu/g for chicken pane and 7.46x104 ± 1.7x104,1.2x102 ± 2.1x10 and 7.2x103 ± 2.3x103cfu/g for chicken nuggets. Moreover, the incidence of S.entretidis was 6.6% for chicken strips and not detected in pane and nuggets. Staph aureus incidence was 20%, 26.6%and 26.6% for chicken strips, pane and nuggets for total examined samples by using conventional culture method. For more confirmation, identification of Staph.aureus from 5 random samples by Vitek 2 compact system was applied. Results recorded was identical to that of culture method with accuracy 100% for examined chicken products samples and showed that the Vitek 2 system is a suitable tool for rapid and direct identification of gram positive cocci as an ideal detection system should include high specificity and sensitivity; fast response time; capability for mass production; simplification of sample preparation steps ;minimal perturbation of sample;and providing continuous data analysis as that obtained by the Vitek 2compact system. The public health importance of isolated microorganisms and recommended point were discussed.}, keywords = {Chicken products,Conventional Methods,VITEK 2}, url = {https://bvmj.journals.ekb.eg/article_112173.html}, eprint = {https://bvmj.journals.ekb.eg/article_112173_fd06952a1cf8158ce1e7d0095733eb7e.pdf} } @article { author = {meselhy, amira and Hassan, Mohamed and Amin, Reham}, title = {Assessment Of Chemical Quality Of Local Frozen cattle's Liver}, journal = {Benha Veterinary Medical Journal}, volume = {38}, number = {2}, pages = {97-100}, year = {2020}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2020.22607.1153}, abstract = {The present study was conducted on 120 random samples of frozen local cattle livers (30 of each) each) collected from different slaughter shops and supermarkets in Qalyobia Governorate, for estimation of their keeping quality(pH, TBA, TVN, PV) and determination of the heavy metals residues (lead, cadmium, arsenic and copper) and their acceptability for human consumption. The results revealed that for frozen local livers collected from slaughter shops; the mean values of PH ,TVN, TBA and PV concentrations "mg/Kg" were 6.19 ± 0.01; 11.67 ± 0.54; 0.26 ± 0.03 and 0.55 ± 0.04. while those collected from supermarkets, the mean values of PH ,TVN, TBA and PV concentrations "mg/Kg" were 6.35 ± 0.02; 8.25 ± 0.41; 0.17 ± 0.02and0.37± 0. The results revealed that for frozen local livers collected from slaughter shops; the mean values of lead, cadmium, arsenic and copper, concentrations "mg/Kg" were 0.31 ± 0.02; 0.23 ± 0.02; 0.17 ± 0.01 and3.81 ± 0.35,respectively .while frozen local liver samples collected from supermarkets ; the mean values of lead ,cadmium ,arsenic and copper concentration "mg/Kg" were 0.19 ± 0.02 ; 0.14 ± 0.01; 0.10 ± 0.01and 3.65 ± 0.28, respectively . The study concluded that, frozen local cattle liver products have public health importance as the residues in them, might exceed the recommended safe permissible limits.}, keywords = {cattle liver(frozen local )PH,tba,TVN,PV,lead cadmium arsenic and copper}, url = {https://bvmj.journals.ekb.eg/article_112181.html}, eprint = {https://bvmj.journals.ekb.eg/article_112181_54cf9e2bebc581f6e99bacbe25a819bf.pdf} } @article { author = {abd ellatif, zakaria and Saad, Saad and Hassanin, Fatin and Salem, Amani and Saleh, Ebeed}, title = {Bacteriological profile of sheep carcasses in a private Egyptian abattoir}, journal = {Benha Veterinary Medical Journal}, volume = {38}, number = {2}, pages = {101-105}, year = {2020}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2020.27497.1196}, abstract = {Abstract Sheep are considered one of important protein sources in Egypt; due to its availability, nutritional value, and desirable taste, mutton and its edible offals are of good consumer demands. Hygienic profile of the slaughter house facilities and personnel handle fresh carcasses play the major role of its safety and compatability for human consumption. Therefore, this study aimed to detect the bacteriological profile of ninety random fresh samples of sheep meat, liver, and lungs (30 of each) collected from immediately eviscerated sheep carcasses in private slaughter houses in Eastern-Owinat, New Valley province, Egypt. Assessment of the bacteriological quality of the examined samples was conducted to investigate their hygienic quality and safety for human consumption. Results showed variable contamination levels in accordance of mean values of aerobic plate counts (APC), Enterobacteriacae counts (EC), coliform counts (CC), and Staphylococcus counts (SC). Accordingly, the liver samples were the highest contaminated samples with APC, EC, and CC with mean values of 3.06x105, 2.1x104, and 1.2x104 CFU/g, respectively; while mutton samples were the most contaminated with SC with incidence and mean value of 8.0x10 (46.66%). Referring to C. perferingens, it was detected in 13.3% of the examined liver and lung samples, and 16.66% of examined meat samples; with mean values of 1.7x10, 1.3x10, and 1.5x10 CFU/g, respectively. The obtained results indicated that fresh mutton and edible offals may pose a risk to consumer’s health in the absence of food safety knowledge and hygiene application, therefore, strict hygienic measures and authority inspection is strongly recommended.}, keywords = {Bacteriological Quality,Sheep carcass,Egyptian private slaughterhouse}, url = {https://bvmj.journals.ekb.eg/article_112182.html}, eprint = {https://bvmj.journals.ekb.eg/article_112182_cc3489e199d6aa071cfca9ae3adc212c.pdf} } @article { author = {Shehab ElDin, Suhair and Saad, Saad and Ibrahim, Hemmat and Hassan, Mohamed}, title = {Molecular detection of virulence factors in some food poisoning bacteria isolated from chicken meat and giblet}, journal = {Benha Veterinary Medical Journal}, volume = {38}, number = {2}, pages = {106-111}, year = {2020}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2020.31024.1211}, abstract = {Many bacterial detection rapid methods developed including nucleic acid based analysis which considered the most precise, sensitive, and famous method of detection. This study aimed to investigate the bacterial hygienic quality of some chicken meat and giblet with special concern of molecular detection of some virulence factors associated with some isolated food poisoning bacteria. E. coli, Salmonella, S. aureus, and Y. enterocolitica strains were isolated from commercial and home-reared chicken meat and giblet in Menoufiya Governorate, Egypt. Accurately, stx1, stx2, eaeA, and hylA genes were detected in 45.4, 63.6, 18.1, and 27.2% of the isolated E. coli strains, respectively. invA, hilA, and fimH genes were detected in 100, 71.4, and 85.7% of the examined Salmonella isolates, respectively. Regarding to the examined Y. enterocolitica isolates, Inv gene was detected lonely in 25%, while it was mixed with ystA gene in 75% of the examined isolates. Detection of enterotoxigenic Staph. aureus genes revealed detection of staphylococcal enterotoxins genes types SEA, and SEB genes in 20, and 10%; moreover, mixed SEA+SED, SEB+SEC producing genes were detected in 10% for each, respectively. The present results proved that PCR assay is helpful, rapid and accurate detection method. Strict hygienic measures during slaughtering and handling of chicken meat and giblet must be followed.}, keywords = {PCR,S. aureus,E. coli,S. typhi,chicken carcass}, url = {https://bvmj.journals.ekb.eg/article_112694.html}, eprint = {https://bvmj.journals.ekb.eg/article_112694_154707c64627e6eecf8c11d8e6013168.pdf} } @article { author = {Edris, Abo Bakr and Ebraheem, Ekbal and Elsheewy, Ahmed}, title = {Biocontrol of food contamination}, journal = {Benha Veterinary Medical Journal}, volume = {38}, number = {2}, pages = {112-115}, year = {2020}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2020.33527.1219}, abstract = {It is first important to understand what the potential hazards are when it comes to food safety. Food contamination refers to food that has been corrupted by another substance – either physical, biological or chemical. Biological contamination refers to food that is contaminated by organisms or substances they produce. This includes biological matter produced by humans, rodents, insects and microorganisms. Bacteria and viruses are typically the two biggest causes of biological contamination and can result in some of the most common types of food poisoning including salmonella, E .coli, listeria and Norovirus. Thoroughly washing your hands and sanitising the food handling equipment are two of the best ways to prevent against bacterial contamination. Physical contamination is when a foreign object contaminates food. This can happen at any stage of the production process and could include glass, steel wool or pieces of plastic. Chemical contamination refers to food that has been contaminated with a natural or artificial chemical substance. These contaminants are particularly dangerous as they expose people to any number of toxic substances, some of which can be fatal.}, keywords = {Biocontrol,Contamination,prebiotics,probiotics,bacteriophage}, url = {https://bvmj.journals.ekb.eg/article_112695.html}, eprint = {https://bvmj.journals.ekb.eg/article_112695_fa7ec37d6c8d37669962d3847f0523fc.pdf} } @article { author = {abd ellatif, zakaria and Saad, Saad and Hassanin, Fatin and Salem, Amany and Saleh, Ebeed}, title = {Efficiency of some organic acids as decontaminants in sheep carcasses}, journal = {Benha Veterinary Medical Journal}, volume = {38}, number = {2}, pages = {116-119}, year = {2020}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2020.27504.1197}, abstract = {Abstract Potent and food-safe antibacterial chemicals capable of decontaminating carcass surfaces have been researched for a long time due to their great value for the quality and shelf life of the meat. The aim of current study is to test the antibacterial effect of lactic acid (LA) and acetic acid (AA) spray treatments using three concentrations (1, 1.5 and 2 %) on the aerobic plate count, Enterobacteriacae count, coliform count, and Staphylococcus count of fresh sheep carcasses surface after 20 minutes of spraying. Results of the investigated bacteriological parameters showed significant reductions after being exposed to organic acids, especially Gram negative bacteria (Enterobacteriacae) which showed greater sensitivity to the used organic acids than Gram positive bacteria (Staphylococcus), where greater concentration gave greater reduction in the bacterial counts. Moreover, spray wash of lactic acid resulted in higher reduction of bacterial counts on meat surface than acetic acid. From the obtained results, organic acids showed safe, simple, efficient, cheap, and highly effective modality of meat decontamination, on addition, application of lactic acid 2.0% spray showed higher anti-bacterial effect, therefore, it is recommended to improve safety of sheep carcasses for industrial scales.}, keywords = {acetic acid,lactic acid,Sheep carcass,Bacteriological Quality}, url = {https://bvmj.journals.ekb.eg/article_112813.html}, eprint = {https://bvmj.journals.ekb.eg/article_112813_49a566ed5f75df528c277e9d3148f4f7.pdf} } @article { author = {Abd El-Tawab, Ashraf and Elhofy, Fatma and Amin, Sohila}, title = {Phenotypic and genotypic characterization of Candida albicans isolated from chicken}, journal = {Benha Veterinary Medical Journal}, volume = {38}, number = {2}, pages = {120-124}, year = {2020}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2020.25501.1185}, abstract = {Candidiasis is an occasional opportunistic yeast disease of importance in poultry and it has also been reported to be a disease or an intestinal infection. The present study was directed mainly to throw lights firstly on the isolation of C. albicans species of chicken and secondly to the identification of the mentioned isolates by phenotypic and genotypic methods. A total 100 of swab samples collected from a crop of chicken from different localities in Cairo and Giza governorate. The samples were investigated for yeast species contamination and mycological examination, which the isolation of four yeast genera. The most frequent yeast species isolated from such samples followed by Candida spp., Saccharomyces spp., Torulopsis spp. and Rhodotorula spp. The observe incidence of Candida species isolated from all examined samples it seems that Candida species were (n=36/60, 60%) from chicken crop swab.The incidence of Candida albicans isolated from examining samples it seems (n=8/36, 22.2%) from crop swab. Early identification of yeast to the genus and species level is necessary for effective antifungal therapy, and can also facilitate control of infections. Explained that Cross-contamination is a particular problem and several recommendations have been published to control pathogens.}, keywords = {C.albicans,Crop,Rap ID system,LH1,LH2}, url = {https://bvmj.journals.ekb.eg/article_112696.html}, eprint = {https://bvmj.journals.ekb.eg/article_112696_706ba2ba5abe78c8b6bac8f7fb608e9f.pdf} } @article { author = {Mousa, Dina and Abd El Tawab, Ashraf and El Hofy, Fatma and Maarouf, Ahmed}, title = {Molecular studies on antibiotic resistant Bacillus cereus isolated from meat products and human in Kaliobia, Egypt}, journal = {Benha Veterinary Medical Journal}, volume = {38}, number = {2}, pages = {125-130}, year = {2020}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2020.25802.1187}, abstract = {The current study was conducted on 210 random samples of meat products (beef burger, kofta, luncheon, minced meat, sausage) and diarrheic human stool of patients suffering from vomiting and diarrhoea (35 for each). The meat products were collected from different shops and hospitals at Kaliobia Governorate, Egypt, for detection of B. cereus strains, and their phenotypic characterization as well as antibiotic resistant genes. Bacteriological examination of the collected samples indicated the identification of 51 (24.3%) isolates of B. cereus from 210 samples as 11/31.4% from kofta 13/37.1% from minced meat; 9/25.7% from sausage; 7/20.0% from beef burger; 6 /17.1% from luncheon samples; and 5/14.3% from human stool specimens. Most of 51 isolated B. cereus strains had the ability for biofilm production. The antibiotic sensitivity profiles revealed that, the isolated B. cereus were highly resistant for Penicillin-G followed by methicillin; ampicillin; oxytetracycline; sulfa-trimethoprim and cefotaxime. Meanwhile, they were highly sensitive to to gentamycin and norfloxacin followed by ciprofloxacin; meropenem and florphenicol. Further, PCR declared that bla; tetA and erm genes were amplified in 9; 7; 6 out of 10 studied B. cereus isolates giving products of 680 bp.; 502 bp. and 645 bp. respectively. Therefore, one can conclude that; B. cereus especially antibiotic resistances ones are meat-borne pathogens of public health importance and they may be the causative agents in patients suffering from vomiting and diarrhoea.}, keywords = {meat products,B. cereus,antibiotic resistant genes,human}, url = {https://bvmj.journals.ekb.eg/article_112697.html}, eprint = {https://bvmj.journals.ekb.eg/article_112697_46056b8c4b11b4e92f3f119846e95105.pdf} } @article { author = {Sedek, Amany and Abd El Tawab, Ashraf and El-Hofy, Fatma and El-Gohary, Mohamed}, title = {Antibiotic resistance genes of Edwardesiella tarda isolated from Oreochromis niloticus and Claris gariepinus}, journal = {Benha Veterinary Medical Journal}, volume = {38}, number = {2}, pages = {131-135}, year = {2020}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2020.29323.1205}, abstract = {Frequent use of antimicrobial agents may result in bacterial generation resistant to multiple antibiotics that affect on public health hazard. Our Study aimed to isolation of Edwardesiella species from fresh Oreochromis niloticus and Claris gariepinus(50 of each ) which were collected from fish farms and markets in Kafrelsheikh governorate. Atotal of 450 tissue samples involving liver, kidney, spleen, intestine from both fish species and gills from O. niloticus. Isolation and identification of the bacterial pathogens by traditional methods then antibiotic sensitivity test and resistance gene were detected. 23 isolates were obtained and distribution of Edwardesiella spp. among examined organs indicated that 9/100 (9%) isolates were detected from spleen, 6/100 (6%) from liver, 5/100 (5%) from intestine, 2/100 (2%) from kidney and 1/50 (2%) from gills. The predominant species were E. tarda so, 8 isolates were tested for 13antibiotic agents. The resistance were recorded in all 8 strains for Amoxicillin and Flumox, in 7 from 8 strains for Ampicillin, 6 from 8 for Cefotaxime, in 2 from 8 for Oxytetracycline and Streptomycin . Resistance profile genotypically in 3 isolates for β-lactamases( blaTEM, blaCTX) aminoglycosides (aada1)and tetracyclines (tetA(A))genes were 100% in all isolates. In conclusion presence of antibacterial resistance indicates misuse of antibacterial agents and affect on public health.}, keywords = {E. tarda,beta lactamases,O. niloticus,resistance genes}, url = {https://bvmj.journals.ekb.eg/article_112699.html}, eprint = {https://bvmj.journals.ekb.eg/article_112699_eb7ffade925d8ad614dd16a53fee4f6b.pdf} } @article { author = {Abd El Tawab, Ashraf and Elhofy, Fatma and Moustafa Moustafa, Eman and Halawa, Marwa}, title = {Isolation and Molecular Identification of Aspergillus species from Cultured Nile Tilapia (Oreochromis niloticus)}, journal = {Benha Veterinary Medical Journal}, volume = {38}, number = {2}, pages = {136-140}, year = {2020}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2020.27514.1198}, abstract = {The present study was carried out on 120 examined cultured Nile tilapia, Oreochromis niloticus (O. niloticus) (80 diseased fish and 40 apparently healthy) were collected alive from private fish farms at Kafr El Sheikh Governorates from April to October 2018. Grossly, examined diseased O. niloticus revealed eroded fins, redness on the skin, congested protruded anal opening and sometimes darkness on skin, ulcers with different sizes, and eye turbidity especially in fish naturally infected with Aspergillus, Fusarium and Pencillium. Mycological examination revealed isolation of 7 Genera of fungi from total incidence of 300 fungal isolates; 240 fungal isolates from diseased O. niloticus and 60 isolates from apparently healthy one. Aspergillus flavus and Aspergillus niger were the most predominant fungi isolated from either apparently healthy or even diseased one mainly from internal organs. Molecular identification was performed using inter-transcriped spacer (ITS) gene for A. flavus and A. niger. Developed PCR assay specific primer detect A. flavus and A. niger showing clear bands at 595 and 600 bp molecular weight, respectively.}, keywords = {Asperigillus,identification,Oreochromis niloticus,PCR}, url = {https://bvmj.journals.ekb.eg/article_112700.html}, eprint = {https://bvmj.journals.ekb.eg/article_112700_38096f5fedf26302ff28a2eb83e1768a.pdf} } @article { author = {Abd El-Tawab, Ashraf and El-Hofy, Fatma and EL-Gamal, Refaat and Awad, Somayah and El-Mougy, Emad and Mohamed, Samaa}, title = {Phenotypic and genotypic characterization of Antibiotic resistant strains of Flavobacterium columnare isolated from Oreochromis niloticus (Nile tilapia)}, journal = {Benha Veterinary Medical Journal}, volume = {38}, number = {2}, pages = {141-145}, year = {2020}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2020.25826.1188}, abstract = {Flavobacterium columnare (F.columnare) is an important pathogen affecting gills and skin of freshwater fishes causing columnaris disease. The objective of the study to isolate F.columnare from Nile tilapia and study the prevalence of antibiotics resistance strains with genotypic identification of β-lactamase resistance genes (blaTEM, bla SHV) and tetracycline resistance gene (tetA) among isolated strains of F.columnare. Two hundred diseased fish were randomly collected from different localities throughout year (2018-2019). F. columnare showed seasonal prevalence 0.00%, 10%, 40.00%, and 20.00% in winter, spring, summer and autumn respectively with total prevalence 35/200 (17.5%). F.columnare was isolated in high percent from skin and gills and rare in internal organs. The invitro antibacterial sensitivity test was applied on 20 F.columnare isolated strains and revealed resistant to pencillin, cephalosporin, aminoglycoside, nitrofurans, polymixinB and tetracycline. The isolated strains have three antibacterial resistance genes, bla TEM with amplicon size 516bp, bla SHV with amplicon size 392bp and tetA with amplicon size 576bp. Conclusion: According to this study tested strains were resistant to multiple antibiotics and their genes were widely distributed, so phenotypic and genotypic characterization of antibiotic resistant strains of isolated F. columnare are similar.}, keywords = {Flavobacterium columnare,Oreochromis niloticus,prevalence,antibacterial resistant,Gene}, url = {https://bvmj.journals.ekb.eg/article_112701.html}, eprint = {https://bvmj.journals.ekb.eg/article_112701_2604205b69abb2d80fb298b5e3d475af.pdf} } @article { author = {gamal, nesma and Abd El-Tawab, Ashraf and Elhofy, Fatma and Maarouf, Ahmed}, title = {Phenotypic characterization of some food poisoning bacteria isolated from meat and meat products in Kaliobia, Egypt}, journal = {Benha Veterinary Medical Journal}, volume = {38}, number = {2}, pages = {146-151}, year = {2020}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2020.25678.1186}, abstract = {The present study was performed on 250 random samples of fresh meat and meat products viz: Beef burger, kofta; minced meat and sausage (50 for each), were collected from different shops (25gm from each sample) at Kaliobia Governorate, Egypt, for detection the prevalence of some toxigenic food-borne bacteria , beside the phenotypic characterization and detection of some virulence genes . Bacteriological examination of the collected samples resulted in, isolation of 90 (36.0%) isolates of S. aureus isolates (41/16.4%); E. coli (25 /10.0%); B. cereus (21/8.4%) and Salmonella (3/1.2%) were recovered from 250 samples. ,Seven serogroups (O55:H7; O111:H4; O125:H18; O126:H7; O128:H27; O142:H2and O158:H2) were detected in 25 isolated E. coli strains. The antibiotic sensitivity tests for the isolated strains show multiple antibiotic resistances (ampicillin; methicillin; oxytetracycline; amoxicillin; streptomycin; erythromycin; doxycycline and cefotaxime). Therefore, E. coli; S. aureus and B. cereus strains especially antibiotic resistances ones are meat-borne pathogens of public health important.}, keywords = {meat products,food borne pathogen,antibiotic resistant}, url = {https://bvmj.journals.ekb.eg/article_112702.html}, eprint = {https://bvmj.journals.ekb.eg/article_112702_bf7243999c07cd6115af8d5aeb6a1b11.pdf} } @article { author = {Abd El-Tawab, Ashraf and Elhofy, Fatma and shawky, nahla and el morsy, doaa}, title = {Molecular detection of antibiotic resistant bla gene in B. cereus isolated from meat products}, journal = {Benha Veterinary Medical Journal}, volume = {38}, number = {2}, pages = {152-155}, year = {2020}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2020.27904.1199}, abstract = {In this study the incidence of Bacillus cereus (B. cereus) and antibiotic sensitivity test was undertaken. A total of one hundred random samples of meat products of rice kofta, kobeba, chicken pane and chicken nuggets (25 of each) were collected from different supermarkets at different times in Menofiea and Kalyobia governorates, Egypt and tested for occurrence of Bacillus group. Bacillus cereus were detected in 24%, 12%, 20% and 10% of kobiba–shami , rice kofta, chicken pane and chicken nuggets ,respectively . Bacillus mycoids failed to be isolated from kobiba–shami and isolated from 4%, 16% and 4% of rice kofta, chicken pane and chicken nuggets, respectively . Also, B. thuringenesis failed to be isolated from kobiba–shami and rice kofta while it isolated from 8% of both chicken pane and chicken nuggets. Fourteen strains of B. cereus were examined for antimicrobial susceptibility testing. The foremost common drug resistance was to penicillin, amoxicillin and amoxicillin+clavulanic (100% for each). On the other hand, B. cereus was completely prone to vancomycin and gentamycin (100% for each).Using PCR, all tested B. cereus isolates harbored bla gene.}, keywords = {B. cereus,chicken and meat product,bla resistance gene}, url = {https://bvmj.journals.ekb.eg/article_112698.html}, eprint = {https://bvmj.journals.ekb.eg/article_112698_ee3898d4103a6789cd7de7717730071a.pdf} }