@article { author = {Ramadan, Sherif and Saker, Abdel-Aziz and Shafik, Basant}, title = {DNA polymorphism of FSHR gene and its association with infertility traits in Egyptian buffaloes}, journal = {Benha Veterinary Medical Journal}, volume = {39}, number = {1}, pages = {1-4}, year = {2020}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2020.34139.1221}, abstract = {The objectives of this study were to detect genetic polymorphism in exon 10 of Follicle Stimulating Hormone Receptor gene (FSHR) by using RFLP and direct sequence methods, also to investigate their possible association with infertility traits in Egyptian buffaloes. A total number of 50 female Egyptian buffaloes at Mahallet Mousa experimental farms in Kafr El-sheikh province were investigated. Animals were divided into three groups: normal fertile (20 animals), infertile due to anestrum (15 animals) and infertile due to repeat breeding (15 animals). Genomic DNA was extracted from blood samples, then PCR was performed with annealing temperatures of 57ºC to amplify 231 bp of FSHR exon 10 gene. The amplicons were digested with Hin1II (NlaIII) restriction enzyme. The results showed that all the investigated buffaloes were monomorphic and genotyped as A/A genotype. Moreover, the monomorphism of buffaloes FSHR exon 10 gene was confirmed by sequencing three samples from each buffaloes’ group. It seems that FSHR gene are highly conserved in the investigated buffalo’s population.}, keywords = {Egyptian buffaloe,fertility,FSHR,Polymorphism,RFLP}, url = {https://bvmj.journals.ekb.eg/article_116439.html}, eprint = {https://bvmj.journals.ekb.eg/article_116439_f2c35cc95520698d9790639163fc9a5a.pdf} } @article { author = {WEHABA, MOHAMMED}, title = {The occurrence of pathogenic E. Coli in some types of soft cheeses in the local market}, journal = {Benha Veterinary Medical Journal}, volume = {39}, number = {1}, pages = {5-10}, year = {2020}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2020.37011.1230}, abstract = {The purposes of this practical research to detect the incidence of pathogenic E. coli in kariesh, domiatti and tallaga cheeses. Confirmative serological identifications of the isolated strains of pathogenic E. coli were performed. One hundred and fifty random samples of kariesh, domiatti and tallaga cheeses (50 each) were collected from different shops and street vendors in El Gharbia territories. Samples were bacteriologically examined for the presence of E. coli. The incidence of E. coli in the analyzed samples was 54%, 42%, and 32%, respectively. Serotyping of isolated pathogenic serovars of E. coli were: O119, O111, O86 & O128, and O124 in domiatti cheese. Genotypic identification of E. coli virulence genes (Stx1, eaeA, fimH) were recorded at percentage of Zero %, 28.5% and 100 % respectively. In kariesh cheese. the detected E. coli in the analyzed samples surely able to causes public health disease outbreaks to the consumers and stipulated the local hygienists to insures maximum health’s stipulations. Therefore this work was planned to covers the followings points:1-Isolations, biochemical and serological identifications of pathological Escherichia coli from kariesh, tallage, and domiatti types of cheeses in El _Gharbia governorate. 2-Molecular identifications of isolated bacteria (bacterium coli) using the PCR technique.}, keywords = {E. coli,selective isolation,in vitro sensitivity,Virulence genes,Soft cheese}, url = {https://bvmj.journals.ekb.eg/article_116440.html}, eprint = {https://bvmj.journals.ekb.eg/article_116440_e97dcd8822038c593dbbb3eb5918e3bf.pdf} } @article { author = {Afify, Sayed and Shaltout, Fahim and Mohammed, Islam}, title = {Bacteriological profile of some raw chicken meat cuts in Ismailia city, Egypt}, journal = {Benha Veterinary Medical Journal}, volume = {39}, number = {1}, pages = {11-15}, year = {2020}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2020.37176.1232}, abstract = {One hundred random samples of raw chicken meat cuts represented by breast and thigh (50 of each) were collected from an automatic poultry slaughtering plant in Ismailia city, Egypt. The samples were examined to investigate their bacteriological aspect, where the mean values of aerobic plate count and Enterobacteriacae were 5.9x105 and 5.1x104 CFU/g for breast samples and 7.1x105 and 6.1x104 CFU/g while for thigh samples as well as the incidence of Enterobacteriacae contaminated the breast and thigh samples were 42 and 54%, respectively. Moreover, S. aureus, Salmonellae, E. coli, Enterobacter spp., Proteus spp., Shigella spp. and C. perfringens were detected in 10, 14, 12, 4, 2, 2 and 16% of the examined breast samples, respectively. Referring to thigh samples, S. aureus, Salmonellae, E. coli, Klebsiella spp., Enterobacter spp., Proteus spp., and C. perfringens were detected in 4, 8, 18, 4, 2, 2 and 10%, respectively. The obtained results indicated that the raw chicken meat cuts may harbor many food poisoning bacteria from different sources, which strongly recommends following strict hygienic measures through slaughtering, handling and cooking processes.}, keywords = {Chicken meat,Breast,thigh,Bacteriological aspect}, url = {https://bvmj.journals.ekb.eg/article_116441.html}, eprint = {https://bvmj.journals.ekb.eg/article_116441_403b2550616a1f901c7453f4eaa82748.pdf} } @article { author = {Hashem, Hend and Hussein, Samy and El senosi, Yakout}, title = {Lycopene mitigates experimental colitis in rats by inhibiting oxidative stress-mediated inflammation and apoptosis}, journal = {Benha Veterinary Medical Journal}, volume = {39}, number = {1}, pages = {16-21}, year = {2020}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2020.37614.1236}, abstract = {Objective: The potential protective and beneficial effects of Lycopene (LYC) in a rat model of acetic acid-induced ulcerative colitis (UC) were evaluated. Methods: Forty male albino rats were divided into five equal groups. Group I: (Control normal) rats received no drugs. Group II: (Early ulcerative colitis): rats received 2 ml (3% v/v) glacial acetic acid intracolonially at 21 th day of experiment and sacrificed 3 days later. Group III:(Early ulcerative colitis + Lycopene protected) rats received Lycopene (10 mg/kg body weight/day, orally) for 21 days prior to acetic acid administration. Group IV: (Late ulcerative colitis) rats received acetic acid similar to group II for 3 successive days and sacrificed after 21 days. Group V: (Late ulcerative colitis+ Lycopene treated) rats first administered with acetic acid then after 3 days Lycopene was administered for 21 days. Results: A significant increase in L-Malondialdehyde (L-MDA) with marked decrease in reduced glutathione (GSH) level and Catalase (CAT) activity were observed in colon tissue of UC-induced rats. Additionally, a significant up-regulation of nuclear factor kappa B (NF-κB), caspase-3 and Transforming growth factor-β1 (TGF-β1) and down-regulation of Interleukin -10 (IL-10) and Beta cell lymphoma-2 (Bcl-2) gene expression levels of were observed in colon of UC induced rats. However, a significant depletion of colon tissue L-MDA and down-regulation of NF-κB, caspase-3 and TGF-β1 in addition to marked increase in GSH concentration and CAT activity and up-regulation of IL-10 and Bcl-2 were observed after LYC treatment. Conclusions: Lycopene has powerful antioxidants, anti-inflammatory and anti-apoptotic effects against ulcerative colitis.}, keywords = {lycopene,colitis,Oxidative Stress,Anti-apoptotic and Inflammatory cytokine}, url = {https://bvmj.journals.ekb.eg/article_116442.html}, eprint = {https://bvmj.journals.ekb.eg/article_116442_e936f7494a47904ad8f25299d68f546c.pdf} } @article { author = {El-ghamry, Eman and karosah, Mohamed and el-gendi, gaffar and ahmed, essam}, title = {Effect Of Some Feed additives (Yeast, Fenugreek seeds and Chamomile flowers) On Some Behavioral patterns and Productive Performance In Pigeons (Columbia livia domestica).}, journal = {Benha Veterinary Medical Journal}, volume = {39}, number = {1}, pages = {22-27}, year = {2020}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2020.36464.1226}, abstract = {This work was carried out at the research center belonging to faculty of agriculture, Benha university through the period from April to the end of September2019 to study the effect of some feed additives (Yeast, Fenugreek seeds and Chamomile flowers) on some behaviors and productive performance of parent pigeons using 16 pairs of hybrid pigeons (Carrier x local Egyptian baladi) with age of 6 months, they were distributed according to their mating system (sex ratio 1:1). Pigeons were divided into 4 treatments each of them composed of 4 pairs and were reared under the same managerial conditions. Obtained results revealed that pigeons fed on Yeast had high frequency of feeding, drinking and sitting on egg also, it increased length of incubation period while, it decreased hatchability %. Fenugreek seeds had high frequency of preening and sexual sitting on egg but, it decreased length of incubation period. Both Fenugreek and chamomile flowers improved hatchability %. From this work, it can be concluded that addition of some feed additives (Yeast, Fenugreek seeds and Chamomile) had the ability to improve behaviors, reproductive and productive performance of adult pigeons.}, keywords = {Feed additives,Pigeons,Behavior,reproductive,productive performance}, url = {https://bvmj.journals.ekb.eg/article_116443.html}, eprint = {https://bvmj.journals.ekb.eg/article_116443_261d6813587750835e06bc2018026f56.pdf} } @article { author = {Antar, Reham and shosha, saad and elazab, mohamed and esmail, Randa}, title = {Genetic and Hormonal Difference between High Growth Rate Breed (Cobb Broiler chicken) and Low Growth Rate Breed (Native Fayoumi chicken)}, journal = {Benha Veterinary Medical Journal}, volume = {39}, number = {1}, pages = {28-33}, year = {2020}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2020.36634.1229}, abstract = {Abstract The present study aimed at investigating the genetic and hormonal differences between a low growth rate chicken breed (a native breed: Fayoumi chicken) and a high growth rate chicken breed (Cobb broiler chicken). A total number of 100 one-day-old Fayoumi chicks and 100 one-day-old Cobb chicks were used. Chicks of each breed were allocated into three equal replicates. The growth parameters (body weight, BWG and FCR), genetic expression of some growth related genes (GH, IGF1, ghrelin and Myostatin gene) and plasma level of some growth related hormones (Insulin, T3, T4 and corticosterone) were recorded. The obtained results revealed that, Cobb chicken had significantly higher body weight, BWG and feed intake than Fayoumi chicken but the FCR of Fayoumi chicken was higher than that of broiler chicken. Cobb recorded significantly lower plasma level of corticosterone, T3 and T4 than Fayoumi chicken while Insulin is higher in cobb chicken. Cobb recorded highly significant values of GH, IGF1 and ghrelin Gene expression while myostatin gene expression was higher in Fayoumi chicken than in Cobb From the obtained results could be concluded that, the observed high growth rate in cobb chicken may be attributed the difference gene expression related growth as well as hormonal difference}, keywords = {Key words:- Fayoumi,Broilers,Growth,Gene expression,corticosterone}, url = {https://bvmj.journals.ekb.eg/article_116444.html}, eprint = {https://bvmj.journals.ekb.eg/article_116444_03a39eba38b742ad8608fa9263886350.pdf} } @article { author = {zahran, reda and Khader, Nasser and ahmed, tahia}, title = {Effect of dietary nucleotide supplementation on broiler performance and economic efficiency}, journal = {Benha Veterinary Medical Journal}, volume = {39}, number = {1}, pages = {34-39}, year = {2020}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2020.37547.1234}, abstract = {A B S T R A C T A total number of two hundred forty-one-day-old broiler chicks (Ross 308) were randomly distributed into four different treatment groups (A, B, C and D), of three replicates (20 chicks / replicate). The chicks in control group (A) were fed negative control diet, group B were fed diet containing (Nucleoforce®) (0.025%) in first 10 days of age, group B were fed diet containing (Nucleoforce®) (0.025%) in first 25 days of age. While group D was fed diet containing Nucleoforce® (0.025%) from zero day till slaughtering. This experiment was designed to investigate the effect of nucleotide as an immunostimulant for broiler chicks in terms of chick performance and economic efficiency. Results showed that, groups fed diet supplemented with Nucleoforce recorded improvements in live body weight, body weight gain and relative growth rate with no significant difference in feed intake and feed conversion index than the control group. Economic efficiency showed non-significant difference between control group and other groups but group (D) recorded high value 0.94 more than control group (A) 0.84. It was concluded that, inclusion of nucleotide 0.025% in broiler diets had a positive role in improvement of final body weight, body weight gain, feed conversion ratio and economic efficiency.}, keywords = {broiler chicks,Nucleotide feeding,Chick performance and Economic efficiency}, url = {https://bvmj.journals.ekb.eg/article_116445.html}, eprint = {https://bvmj.journals.ekb.eg/article_116445_8bfb3009aea45990ac28b6f209b35441.pdf} } @article { author = {Sarhan, Eman and Hussein, Samy and Elsenosi, Yakout and Esmael, Tahia and Amin, Aziza}, title = {Thymoquinone suppressed Cyclosporine A- induced Nephrotoxicity in rats via antioxidant activation and inhibition of inflammatory and apoptotic signaling pathway}, journal = {Benha Veterinary Medical Journal}, volume = {39}, number = {1}, pages = {40-46}, year = {2020}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2020.33389.1217}, abstract = {Cyclosporine A (CsA) is a strong immunosuppressive drug, but its use is frequently accompanied by severe renal toxicity. The potential renoprotective effect of Thymoquinone (TQ) against CsA-induced nephrotoxicity in rats was assessed. Thirty adult white male albino rats were divided into three equal groups. Group I: (Normal control), received no drugs, Group II: (CsA treated), rats received oral dose of Cyclosporine A (25 mg/kg b.wt/day) for 21 days. Group III: (TQ protected + CsA), received Thymoquinone (10 mg/kg b.wt/day) orally 7 days before and during 21 days of CsA treatment. The obtained results showed a significant increase in the concentration of urea and creatinine in serum and L-MDA level in kidney tissue with marked decrease in renal catalase activity and GSH concentration in CsA treated rats. Moreover, a significant down-regulation in Bcl-2 and up-regulation of NF-κB, PAI-1, Caspase-3 and p53 gene expressions levels were observed in kidney tissues of CsA treated rats. Also, various histopathological alterations were detected in kidneys of CsA treated rats. Meanwhile, TQ potentially improved renal function and oxidative alterations related to CsA near its normal ranges. Interestingly, histopathological findings supported that TQ markedly attenuates harmful effects that CsA induced and protected kidney. Our research could conclude that, TQ has an ameliorating role as potent antioxidant, anti-inflammatory and anti-apoptotic agent via inhibition of inflammatory (NF-κB, PAI-1) and apoptotic (Caspase-3, p53) signaling pathway in modulation of CsA-induced nephrotoxicity.}, keywords = {Cyclosporine A,Thymoquinone,apoptosis,Inflammatory cytokines,histopathology}, url = {https://bvmj.journals.ekb.eg/article_116475.html}, eprint = {https://bvmj.journals.ekb.eg/article_116475_493e0eb32503ab4e245b12554d81b2ef.pdf} } @article { author = {Sheir, Suzan and Ibrahim, Hemmat and Hassan, Mohamed and Shawky, Nahla}, title = {Prevalence of Aeromonas spp and their virulence factors isolated from frozen chicken meat products}, journal = {Benha Veterinary Medical Journal}, volume = {39}, number = {1}, pages = {47-51}, year = {2020}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2020.37742.1237}, abstract = {A total of hundred samples of frozen chicken products represented by breast, thigh, nuggets and burger (25 of each) were randomly collected to study the prevalence of Aeromonas spp and their virulence factors in the examined products. the study showed that the mean values of psychrotrophic count were 8.17×103± 1.42×103, 1.95×104± 2.06×104, 3.63×104± 0.89×104 and 7.58×104± 1.16×104, respectively, and the mean values of Aeromonas counts were 9.34×102± 2.01×102, 1.66×103± 0.28×103, 2.90×103± 0.43×103 and 5.25×103± 0.69×103 for examined frozen breast, thigh, nuggets and burger. 12 isolates of A. hydrophila were specific for 16S rRNA gene of which 9 isolates were positive for aerolysin (aerA) and 10 of isolates were positive for haemolysin (ahhl), with incidence of 75% and 83.3%, respectively. The results achieved in the current study showed contamination of chicken products by Aeromonas spp. So in the field of food microbiology it is necessary to give more consideration to Aeromonads because they have the ability of toxins production, survival under low temperatures and growing in a wide spectrum of environments. So, hygienic measures should be adopted to control microbial contamination.}, keywords = {Aeromonas spp,Virulence factors,frozen chicken meat products}, url = {https://bvmj.journals.ekb.eg/article_116447.html}, eprint = {https://bvmj.journals.ekb.eg/article_116447_530fce4b7723c025db67ebc61da521c8.pdf} } @article { author = {mostafa, israa}, title = {ISO 9001 CERTIFICATE AND IMPACT ON FOOD QUALITY}, journal = {Benha Veterinary Medical Journal}, volume = {39}, number = {1}, pages = {52-57}, year = {2020}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2020.36618.1228}, abstract = {ISO 9001:2015 specifies requirements for a quality management system when an organization: a) Aims to enhance customer satisfaction through the effective application of the system, including processes for improvement of the system and the assurance of conformity to customer and applicable statutory and regulatory requirements.There are two main categories of audits: internal and external. Audits are a key component for becoming ISO certified and you must have internal auditors, and pass the 2-stage registrar audit by an external party in order to become ISO 9001 certified. Below we will break down the different ways audits can be conducted and discuss internal, external and certification audits.The three ways audits can be conducted are; On-site audits are performed in full days. The number of days needed for an audit depends on several factors including size, complexity, risk, and nature of an organization. The International Accreditation Forum (IAF) has provided guidelines for registrars to calculate audit time.Remote audits may be performed via web meetings, teleconferencing or electronic verification of processes. Remote audits are less common and typically not as effective as on-site audits. Self-audits do not always mean an internal audit. A self-audit can be requested of your customer to eliminate the need for them to use their resources and still offer some assurance that you are meeting requirements.}, keywords = {Process management,Quality improvement,Manufacturing industries,HACCP}, url = {https://bvmj.journals.ekb.eg/article_116448.html}, eprint = {https://bvmj.journals.ekb.eg/article_116448_506940e519ed944685ddbc4d914a9f8e.pdf} } @article { author = {khalf, elham and Al-Akraa, Adel and abd elgalil, atef and khalil, ahmed and elkassapy, abdelhalim and AlMaghraby, Hussein}, title = {Ultrasonographic Examination of Abdominal Wall Swellings in Cattle and Buffalo (Bubalus bubalis)}, journal = {Benha Veterinary Medical Journal}, volume = {39}, number = {1}, pages = {58-62}, year = {2020}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2020.36528.1225}, abstract = {Abstract A total number of one hundred and fourteen clinical cases (63 cattle and 51 buffaloes) were included in the current study. These cases were suffering from different varieties of abdominal wall swellings (46 abscesses; 49hernias and 19 hematomas) and they were subjected to ultrasonographic examination. From the aforementioned cases the unripend abscess appeared ultrasonographically as a circumscribed echogenic structure with mild distal acoustic enhancement while the ripened abscess appeared as anechoic to hypoechoic structure representing watery to creamy pus contents. The ripened abscess with chronic fibrotic omphalitis revealed a hyperechogenic band with an evident acoustic shadowing. Recent hematomas appeared as anechoic area (blood content) divided by a thin echogenic septa into small chambers toward its center, while old hematoma revealed anechoic to hypoechoic appearance divided by a thick echogenic septa. Reducible hernias revealed disruption of the abdominal wall continuity with intestinal loops and its characteristic peristaltic movement and anechoic, hypoechoic to echogenic contents while irreducible hernias characterized by an evident anechoic inflammatory exudates; reduced peristaltic movement and thick hernial sac with echogenic fibrous adhesions between the sac and the abdominal wall.In conclusion, ultrasonography provides an accurate, non-invasive and fast diagnostic tool for different abdominal wall swellings in cattle and buffalo.}, keywords = {Abdominal wall,Buffalo,cattle,swelling,Ultrasonography}, url = {https://bvmj.journals.ekb.eg/article_116446.html}, eprint = {https://bvmj.journals.ekb.eg/article_116446_856327fa21f126f54d727a22815d14ed.pdf} } @article { author = {Abdelmalek, Walaa}, title = {phenotypic and genotypic characterization of pasteurella multocida isolated from chickens}, journal = {Benha Veterinary Medical Journal}, volume = {39}, number = {1}, pages = {63-67}, year = {2020}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2020.35909.1222}, abstract = {A total of 300 samples from apparently healthy chickens were collected in this study.This samples represented as 100 samples from trachea, 100 samples from lung and 100 samples from heart. They were subjected to morphological and biochemical examination for identification of P.Multocida.An incidence of P. multocida by cultural examination was 21 (7%);- 0% from tracheal swabs, 13% from lung and 8% from heart while an incidence by biochemical examination was 6 (2%) ;- 0% from tracheal swabs - 3% from lung and 3% from heart. All suspected P. multocida colonies were subjected to PCR for identification of P. multocida isolates and proved that six isolates were found to be positive for P.multocida with an incidence of 2% which similar to the results of confirmatory biochemical examination.The drug sensitivity test mentioned that P. multocida was sensitive to ciprofloxacin and norfloxacin.Six suspected isolates of P.multocida identified by cultural examination were tested by polymerase chain reaction method by using specific primers for P.multocida . The stn gene was amplified in four isolates giving 617bp, while the InvA gene was amplified in four isolates giving 284bp.}, keywords = {pasturella multocida,chickens,drug sensitivity test,polymerase chain reaction}, url = {https://bvmj.journals.ekb.eg/article_116449.html}, eprint = {https://bvmj.journals.ekb.eg/article_116449_4f9a60b4c726ff734852bd7c15e047ef.pdf} } @article { author = {el-sharkawy, mohamed and amin, reham and el sabagh, rasha and abo elroos, nahla}, title = {Evaluating the effects of certain probiotics on mycotic contamination in oriental sausage}, journal = {Benha Veterinary Medical Journal}, volume = {39}, number = {1}, pages = {68-72}, year = {2020}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2020.32803.1215}, abstract = {Probiotics are natural antimicrobial agents. It has great beneficial effects to human. In this study, Probiotics as (Lactobacillus casei, Lactobacillus delbrueckii, Bifidobacteriumlactis) were used in oriental sausage to reduce total mould and yeast counts and Aspergillus flavus count. Tow oriental sausage groups were used. The 1st group was used to evaluate reduction %of total mould and yeast count. It was subdivided to 4 groups 3 were inoculated with different Probiotic strains, the 4th was control. Results showed that total mould and yeast count (cfu/g) reduced to (4.73x103±2.33x102, 6.97x103± 2.73x102 and8.80x103 ± 2.08x102) using Lactobacillus casei, Lactobacillus delbrueckii and Bifidobacterium lactis after 12days inducing reduction % of (91.66, 87.71 and 84.48) respectively. The 2nd group was used to evaluate reduction %of Aspergillus flavus count. It was inoculated with Aspergillus Flavus then subdivided to 4 groups 3 were inoculated with different Probiotic strains, the 4th was control. Results showed that Aspergillus Flavus count (cfu/g) reduced to (2.97x104±1.86x103, 5.37x104±1.45x103and 6.34x104± 1.45x103) using Lactobacillus casei, Lactobacillus delbrueckii and Bifidobacterium lactis after 12days inducing reduction % of (92.25, 85.98 and 83.45) respectively. Moreover it was found that Lactobacillus casei was the most effective Probiotics causing the highest reduction% in total mould count and Aspergillus Flavus count followed by lactobacillus delbrueckii then pidfedobacterium lactis.}, keywords = {probiotics,oriental sausage,Aspergillus flavus,Bio preservatives,lactic acid bacteria}, url = {https://bvmj.journals.ekb.eg/article_116450.html}, eprint = {https://bvmj.journals.ekb.eg/article_116450_28c3cf97e230cf99ff63c7734bf5af28.pdf} } @article { author = {Ibrahim, Hemmat and Hassan, Mohamed and Amin, Reham and almokadem, Hend}, title = {E. coli and Salmonella stereotyping in cattle carcasses at abattoir level}, journal = {Benha Veterinary Medical Journal}, volume = {39}, number = {1}, pages = {73-76}, year = {2020}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2020.36055.1223}, abstract = {Ninety random samples of fresh beef were collected from the cattle carcasses slaughtered at 3 different abattoirs in Menoufia governorate namely A, B and C (30 of each), represented by neck region (chuck) during summer and winter seasons in 2018 (45 samples of each season). The obtained results of serotyping of E. coli revealed that EHEC O26: H11 were the more prevalent than other serogroups 6.67%, 6.67% &13.33% and 6.67%, 13.33% & 6.67% .T The results of the total incidence were 46.67% , 40% & 26.67% and 26.67%, 26.67% & 20 for A, B&C abattoirs during the summer and the winter seasons , respectively. Serotyping of salmonella revealed S.Typhimurium was more prevalent during the summer 13.33%for each abattoir, the total isolation rate 33.33%, 26.67% & 20% for A, B and C abattoirs, respectively. The more prevalent serogoup during the winter was S. Enteritidis 13.33%, 6.67% and 6.67%,while the total isolation rate was 26.67% , 20% and 6.67% for A, B &C abattoirs, respectively .The contamination rate increased in abattoir A than B than C and the prevalence was increased during summer than in winter}, keywords = {E.coli,Salmonella,Serotyping,isolation.seasonal variation}, url = {https://bvmj.journals.ekb.eg/article_116451.html}, eprint = {https://bvmj.journals.ekb.eg/article_116451_54b7eece111d1cee654cf34bba485c50.pdf} } @article { author = {almokadem, Hend}, title = {The effect of different disinfectants against some food poisoning bacteria at abattoir level}, journal = {Benha Veterinary Medical Journal}, volume = {39}, number = {1}, pages = {77-79}, year = {2020}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2020.36059.1224}, abstract = {Ninety random samples of fresh beef were collected from cattle carcasses slaughtered at 3 different abattoirs in Menufia governorate (30 sample of each). The sampling site was represented by neck region (chuck) of each carcass in 2018. The obtained results revealed that the efficacy of five different disinfectants against some food poisoning bacteria including E. coli O26: H11, S. Typhimurium and Klebsiella pneumonia which were isolated and serotyped at abattoir level. In vitro infected samples (3.0×106/ cm2) were examined under effect of different concentrations of disinfectants within contact time 120 minute. Disinfectant (A) at concentration 1% was effective against 100% of E. coli O26: H11 and Klebsiella pneumoniae, while 1.5% concentration was required for elimination of 100% S. Typhimurium, followed by B, C, D and E disinfectants, respectively. On the other D and E disinfectants even in 2% concentration and contact time 120 minutes reduce about 99% of the infection.}, keywords = {disinfectants,Food Poisoning Bacteria,Abattoir contamination}, url = {https://bvmj.journals.ekb.eg/article_116452.html}, eprint = {https://bvmj.journals.ekb.eg/article_116452_20dbb23b38c52c1b37185c689e4494a4.pdf} } @article { author = {Hammad, Mahmoud and Ibraheem, Ekbal and Elbarbary, Hend}, title = {Natural antifungal agents for food preservation}, journal = {Benha Veterinary Medical Journal}, volume = {39}, number = {1}, pages = {80-84}, year = {2020}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2020.26306.1191}, abstract = {The increasing societal demand for less processed and more natural food products—while conserving those products’ quality, safety, and shelf-life—has raised the question of chemical preservative replacement. In this context, bacteria and fungi as well as their metabolites are natural alternatives of interest for use in food as bioprotective tools to fight fungal spoilage and to answer consumer demands and legislation.Food spoilage is a major issue for the food industry, leading to food waste, substantial economic losses for manufacturers and consumers, and a negative impact on brand names. Among causes, fungal contamination can be encountered at various stages of the food chain (e.g., post-harvest, during processing or storage. Fungal development leads to food sensory defects varying from visual deterioration to noticeable odor, flavor, or texture changes but can also have negative health impacts via mycotoxin production by some molds. In order to avoid microbial spoilage and thus extend product shelf life, different treatments—including fungicides and chemical preservatives—are used. In parallel, public authorities encourage the food industry to limit the use of these chemical compounds and develop natural methods for food preservation.}, keywords = {Natural antifungal agents,Food preservation,Food spoilage}, url = {https://bvmj.journals.ekb.eg/article_116453.html}, eprint = {https://bvmj.journals.ekb.eg/article_116453_845e2bf5b934785a5ec313290b7a1275.pdf} } @article { author = {elkhalal, dawlat and Alzaghaby, Ehab and attia, hosam and hamad, sherin}, title = {hepatoprotective effect of ethanolic extract of Origanum vulgare against doxycycline toxicity.}, journal = {Benha Veterinary Medical Journal}, volume = {39}, number = {1}, pages = {85-90}, year = {2020}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2020.21307.1160}, abstract = {The current study evaluates the ameliorative action of Origanumvulgareethanolic extract against the hepatotoxic effects of experimental doxycycline overdose in rats. Forty male Sprague-Dawley rats (160–200 gm) were divided into four groups: the control group, administered distilled water (10 ml/kg b.w.); group (2) administered doxycycline (39.2 ml/kg b.w.) in distilled water; group (3) administered O. Vulgare ethanolic extract (20 mg/kg b.w.) +doxycycline (39.2 ml/kg b.w.) in distilled water and group (4) administered O. Vulgare ethanolic extract (60 mg/kg b.w.) + doxycycline (39.2 ml/kg b.w.) in distilled water. All rats were administrated once per day for 4weeks. Doxycycline administrated rats showed alteration in histological structure in the liver as vacuolization, necrosis, inflammation, and sinusoidal dilatations. Also increased Kupffer cells population and congestion and dilatation of central vain with fibrin thrombi filling their lumen were shown.Ultra structurally destructed cell membrane of hepatocyte, Pyknotic and karyolitic nuclei, losses in some cytoplasmic organelles, swollen mitochondria, fragmentation of rER and discarded lysosomes were observed. All of those improved by the ethanolic extract of Origanum vulgare.}, keywords = {rat,Doxycycline,oregano,Liver,histology}, url = {https://bvmj.journals.ekb.eg/article_116454.html}, eprint = {https://bvmj.journals.ekb.eg/article_116454_41508a7ef9afb60b2621ac7b4d58ca47.pdf} } @article { author = {hogoo, hany}, title = {Effect of Different Cooking Methods on Bacteriological Quality of Meat}, journal = {Benha Veterinary Medical Journal}, volume = {39}, number = {1}, pages = {91-94}, year = {2020}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2020.24980.1177}, abstract = {A grand total of 100 random samples of fresh meat collected from different supermarkets and shops from Menoufia governorate. and then divided into four groups, raw, boiled, fried, and roasted evaluated bacteriogically ( total APC, coliform count, St.aureus count, Staphylococcal count) .The bacteriological examination revealed that the mean values of APC of the raw ,boiled ,fried and roasted meat samples were 2.28x107±6.16x106a. ,1.19x103-±2.92x102b. , 1.50x104.± 4.24x103b , 2.70x103- ±1.55x103b cfu/g. .The mean values of coliform count(cfu/g) of raw ,boiled ,fried ,roasted ,meat samples were 1.35x105 ±5.06x104a , 9.2x10±3.00x10b , 2.78x102±8.90x10b ,4.41x102 ±2.08x102b ...The mean value of Staphylococci count(cfu/g) of raw,boiled, fried, roasted meat samples were 7.75x103±2.42x103. 3.48x10±0.11x102b, 0.60x102 ±0.17x102b , 0.92x102±0.27x102b . The mean values of the S. aureus count of the raw ,boiled ,fried ,roasted meat samples were5.12x103± 1.46x103a .,2.62x10 ±0.80x10b , 0.33x102±0.10x102b , 068x102±0.20x102b.Methods of heat treatment of meat have acceptable killing effect on reduction of the bacterial load of raw samples.The boiling method is the first method for reduction of bacterial load of raw meat followed by frying method then roasting method.}, keywords = {boiled meat,fried meat,roasted meat,APC,S. aureus}, url = {https://bvmj.journals.ekb.eg/article_116455.html}, eprint = {https://bvmj.journals.ekb.eg/article_116455_5c9c6a567bc1c9076c5efe767d7e46e4.pdf} } @article { author = {elnahas, khaled and Aziza, Samy and El-Senosi, Yakout and Aggag, Mohsen}, title = {Curcumin and Hesperidin alleviates oxidative stress and hormonal alterations in a rat model of cyclophosphamide-induced premature ovarian failure}, journal = {Benha Veterinary Medical Journal}, volume = {39}, number = {1}, pages = {95-100}, year = {2020}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2020.28289.1200}, abstract = {Premature ovarian failure (POF) is a cessation of ovarian function in women under 40 years of age. Ovarian atrophy leads to a reduced follicle reserve, which leads to menstrual irregularities, ovarian dysfunction, and infertility. The beneficial effects of curcumin (CRC) and hesperidin (HSP) on Cyclophosphamide –induced Premature ovarian failure (POF) in a rat model were studied. POF was induced by intraperitoneal (i.p) injection of cyclophosphamide (200 mg/kg b.wt) at day one, and then (8 mg/kg b.wt/day) for the following 14 days. Two weeks after POF induction, treatment with CRC (100 mg/kg b.wt/day, i.p) and HSP (80 mg/kg b.wt/day, i.p) were started and continued for 14 days. Ninety female rats were divided into six equal groups. Group 1( Control normal group) rats received no drugs, Group 2 (POF-induced group), Group 3 (POF + CRC treated), Group 4 ( POF + HSP treated), Group5 ( POF + CRC+HSP treated) and Group 6 (Normal+CRC+HSP treated). Serum follicle-stimulating hormone (FSH) and ovarian tissues Malonedialdehyde (MDA) concentrations were significantly increased while serum estradiol (E2) level, ovarian superoxide dismutase (SOD) activity and reduced glutathione (GSH) concentration were markedly decreased in POF group as compared with control group. However, a significant increase in serum E2 , ovarian tissues SOD activity and GSH level and marked decrease in FSH and MDA concentrations were observed in rats treated with CRC or/and HSP compared with the POF group. Conclusively, curcumin and hesperidin provided an effective treatment for Premature ovarian failure induced by Cyclophosphamide in rats.}, keywords = {premature ovarian failure,cyclophosphamide,curcumin, hesperidin,Oxidative Stress}, url = {https://bvmj.journals.ekb.eg/article_116456.html}, eprint = {https://bvmj.journals.ekb.eg/article_116456_46cfdf6f1654ad53d983bc49c7aa5ca8.pdf} } @article { author = {Afify, Sayed and Shaltout, Fahim and Mohammed, Islam}, title = {Detection of E. coli O157 and Salmonella species in some raw chicken meat cuts in Ismailia province, Egypt}, journal = {Benha Veterinary Medical Journal}, volume = {39}, number = {1}, pages = {101-104}, year = {2020}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2020.38391.1239}, abstract = {One hundred random fresh raw chicken breast and thigh samples (50 of each) were collected from an automatic poultry dressing plant in Ismailia city, Egypt for bacteriological and genetic detection of E. coli O157:H7 and Salmonella species. Out of the examined 100 samples, only 4 (4%) samples were contaminated with E. coli O157:H7, while non-O157 E. coli isolates represented 11% of E. coli isolates which were serotyped as O114:H21, O127:H6, O126 and O26 with incidence of 2, 4, 3, and 2% of the examined samples, respectively. On the other side, Salmonellae were detected in 11 samples (11%) and serologically identified as S. Typhi, S. Typhimurium and S. Enteritidis with the incidence of 1, 3, and 7%, respectively. Further, PCR investigation indicated that fliC gene was detected in all 4 isolates of E. coli O157 with incidence of 100%; while, fimA gene in 5 Salmonella isolates was detected in 4 (80%) of such examined isolates. Accordingly, it is obvious that raw chicken meat cuts were loaded with pathogenic foodborne bacteria exposing consumers to the high risk of food poisoning. Moreover, the results cleared that bacteriological traditional methods for detection of bacteria contaminating foods are labor-intensive and time-consuming but PCR are more rapid and highly sensitive for identification of foodborne pathogens.}, keywords = {Chicken meat,fliC gene,fimA gene,E. coli O157:H7,Salmonellae}, url = {https://bvmj.journals.ekb.eg/article_116457.html}, eprint = {https://bvmj.journals.ekb.eg/article_116457_784bed60cac4eefd7483de075b674987.pdf} } @article { author = {Mahmoud, Mourad and Abdel-Hamied, Emad and Mahdi, Emad and Sirag, Mahmoud}, title = {Clinico-pathological alterations in Holstein dairy cows with digital dermatitis with therapeutic intervention}, journal = {Benha Veterinary Medical Journal}, volume = {39}, number = {1}, pages = {105-110}, year = {2020}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2020.39119.1244}, abstract = {This study was performed on a commercial dairy herd of 127 Holstein cows with an outbreak of digital dermatitis (DD). Fifty-four DD-affected cows and 20 clinically healthy cows of the same days in milk (DIM) were selected. A detailed examination of each cow feet was recorded. Gross description and anatomic distribution of DD lesions were recorded. Lesions were biopsied and evaluated histopathologically. Lameness scoring was performed. Milk yield and BCS of the DD-affected and healthy cows were compared. Effectiveness of topical oxytetracycline treatment was assessed. The disease was contagious, a total of 54/127 cows were affected in which 70 lesions were identified. The consistent findings were lameness, reduced mobility and a localized painful dermatitis on hind claws. Most cows were clinically lame (62.9%). In the majority (92.6%) of cows only one hind leg was affected, and the left was more affected than the right one (51.9% vs 40.7%). The lesions most commonly (55.6%) were located on the back of hind legs on heel region. Majority of lesions were M2 (44.3%), large size (74.3%), painful (82.9), pink/red (62.9%), deep (42.9%), and circular (60%). Milk yield and BCS significantly decreased in DD-affected cows as compared to healthy cows. Lesions were responsive to topical oxytetracycline treatment. In conclusion, DD is a major cause of lameness in dairy farms and has a negative impact on milk yield and BCS. High incidence of lesions on the back of hind claws is characteristic for DD. Topical oxytetracycline is effective in DD lesions treatment.}, keywords = {Digital dermatitis,clinical score,Pathology,Oxytetracycline}, url = {https://bvmj.journals.ekb.eg/article_116458.html}, eprint = {https://bvmj.journals.ekb.eg/article_116458_a4f17c7088d095b14dc75a00e9032052.pdf} } @article { author = {El Asely, Amel and Youssuf, Hadeer and Abdel Gawad, Eman and Elabd, Hiam and Matter, Aya and Shaheen, Adel and Abbass, Amany}, title = {Insight into summer mortality syndrome in farmed Nile tilapia (Oreochromis niloticus) associated with bacterial infection.}, journal = {Benha Veterinary Medical Journal}, volume = {39}, number = {1}, pages = {111-118}, year = {2020}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2020.40404.1255}, abstract = {The present study was carried out to determine the possible causes of summer mass mortalities among farmed Nile tilapia, Oreochromis niloticus. Pure bacterial colonies were isolated from moribund O. nitoticus from 13 different fish farms; which suffered from high mortalities ranged from (50-80%), during the period from April to October, 2018. Fish showed external hemorrhagic spots, skin darkening, abdominal distension and exophthalmia. Internally, congestion and enlargement of internal organs with serous or hemorrhagic fluid was the most obvious picture. Based on the phenotypic and biochemical characterization using API20E, the isolated bacteria were identified as (A. veronii, A. hydrophila, A. caviae and A. sobria; Aeromonads), (Ps. Fluorescence; Pseudomonas spp), (E. sakazakii and E. cloacae; Enterobacter spp), (C. freundii; Citrobacter spp), (S. odorifera, S. liquefaciens, and S. marcescens; Serratia spp), (S. lutiensis, S. equine; Streptococcus spp), Lactococcus lactis and Proteus vulgaris; with the most prevalence to Aeromonads. Most isolates were accurate identified by PCR and gene sequencing. Water physicochemical parameters were measured at the farm site; which showed an increase in the level of both pH and ammonia. In order to confirm the pathogenicity of the bacterial isolates, an experimental infection was conducted using different doses. The results revealed that A.veronii (HY2) at dose of 9×108 cells/ml was the most pathogenic , with mortality rate 100 %. This study concludes that A. veronii, C. freundii, P.vulgaris and P. fluorescens are implicated in summer mortality of Nile tilapia, without neglecting the role of water quality in worsening the problem.}, keywords = {. niloticus,Mass moralities,gene sequencing,bacterial infection}, url = {https://bvmj.journals.ekb.eg/article_116459.html}, eprint = {https://bvmj.journals.ekb.eg/article_116459_684e333c39e3e993135f818df0495fcc.pdf} } @article { author = {Abd-El Hamed, Amira and Kamel, Eman}, title = {Incidence and Economics of Clinical Mastitis of Holstein Friesian Dairy Cows under Egyptian Condition}, journal = {Benha Veterinary Medical Journal}, volume = {39}, number = {1}, pages = {119-124}, year = {2020}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2020.40775.1259}, abstract = {Mastitis is an endemic disease that is considered to be one of the most frequent and costly dairy disease, so the aim of this study was to investigate different factors that affecting mastitis incidence, and their effect on productivity and profitability of dairy farms. Data used in this study were estimated from 1353 lactation records of Friesian dairy cows within private and governmental farms. The productive, reproductive and economic data for a whole lactation season were recorded for healthy and mastitic cows. Four risk factors for mastitis were included (production sector, calving season, parity and milk production level). Data were classified according to risk factors into two production sectors private and governmental, two calving seasons, summer and winter and Six lactation orders from 1stto 6th, two milk production levels (high and low milk producing cows). Winter calving, older and high producing cows and private sector had the highest mastitis incidence, mastitis reduced the 305MY of Mastitic cows by 33.7 & 20.3Kg during disease period, and 62.7 & 37.7Kg milk was discarded during treatment period for private and governmental sector respectively. Finally economic losses of mastitis per animal was 695.7 EGP yearly.}, keywords = {Key words: Mastitis,Incidence,305My,Economic}, url = {https://bvmj.journals.ekb.eg/article_116460.html}, eprint = {https://bvmj.journals.ekb.eg/article_116460_05c095023edd88e8bc87d6cda85021cc.pdf} } @article { author = {Hassanien, Faten and shaltout, fahim and Fahmey, marionette and Elsukkary, Hassan}, title = {Bacteriological quality guides in local and imported beef and their relation to public health}, journal = {Benha Veterinary Medical Journal}, volume = {39}, number = {1}, pages = {125-129}, year = {2020}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2020.32598.1214}, abstract = {Contamination of meat products are thought to be the most significant key sources of serious diseases, especially foodborne diseases that spreading all over the world. A total of 100 random samples of chilled local and frozen imported beef (50 of each) were collected from local super markets and retail shops from Cairo governorate for evaluation of their quality from bacteriological point of view . Aerobic plate count (cfu/g )in the local samples were between 1.1×106 and 4.4 ×107 with an average of 4.1×107± 0.02×106 while , in frozen imported were ranged between 2.6×107 to 5.3 ×108 with an average of 2.8×107± 0.03 ×107 . Moreover, Coliform count (cfu/g ) in local samples were ranged from 1×103 to 1.2×104 with average of 4.2x103 ± 0.03×103 , while in imported samples were between 6×102 and 11.0×103 with average of 7.1x103 ± 0.02×103 . Eschericia coli were detected in 4% of chilled samples and 2% of frozen samples. Salmonella spp. has been detected in 4% of local samples while all frozen samples were free. From the overall results, we can conclude that both chilled local and frozen imported meat are considered as a significant source of bacteriological public health hazard and need a special control attention.}, keywords = {beef,local,imported,Bacteriological,Evaluation}, url = {https://bvmj.journals.ekb.eg/article_116461.html}, eprint = {https://bvmj.journals.ekb.eg/article_116461_dfa939c05ad2aaab4a0a816ec1e640e7.pdf} } @article { author = {Shehab ElDin, suhair and Saad, Saad and Ibrahim, Hemmat and Hassan, Mohamed}, title = {Antibacterial effect of pepper and cumin extracts on some pathogens contaminating chicken meat}, journal = {Benha Veterinary Medical Journal}, volume = {39}, number = {1}, pages = {130-134}, year = {2020}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2020.31028.1212}, abstract = {Prevention of food poisoning bacteria is usually achieved by using of some chemical preservatives which have public hazards coming through the improper use of it including presence of chemical residues in food, and acquisition of microbial resistance. Based on such safety concerns, the public need to replace it with more potentially effective, healthier natural alternative preservatives are increased. In the present study, two plant extracts, represented by black pepper and cumin extracts were examined to inhibit the growth of foodborne Staphylococcus aureus, Escherichia coli and Salmonella Typhi strains that were artificially inoculated into minced chicken meat samples. Results revealed that the used pepper and cumin extracts significantly reduced the count of tested strains by 102 CFU/g with reduction percent exceeded 99.0%, which proved that the used herbal extracts were potentially effective with variable efficiency against the tested bacterial strains; so, it can be used as natural alternative preservatives to control food poisoning diseases and preserve food stuff avoiding health hazards of chemically antimicrobial agent applications.}, keywords = {black pepper,cumin,Staphylococcus aureus,E. coli,Salmonella Typhi}, url = {https://bvmj.journals.ekb.eg/article_116462.html}, eprint = {https://bvmj.journals.ekb.eg/article_116462_9d521b14beabb3ff94af11f7c515b1ab.pdf} } @article { author = {El-mayet, Fouad and Sharawy, Saad and El-Habbaa, Ayman and Shahen, Nehal and Hagag, Naglaa}, title = {Rapid detection and isolation of Foot and Mouth Disease Virus in samples from clinically suspected animals in Egypt during 2016-2019.}, journal = {Benha Veterinary Medical Journal}, volume = {39}, number = {1}, pages = {135-140}, year = {2020}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2020.30110.1208}, abstract = {Foot and Mouth Disease (FMD) is a highly contagious and economically important disease of susceptible cloven-hoofed animals, mostly for cattle, buffalo and pigs. This study was designed to isolate and identify the serotypes of FMD virus from clinically suspected animals in different localities in Egypt that would be useful to detect the current strains present in Egypt to be used in the future vaccination program. Tongue epithelium, vesicular fluid and heart tissue samples were collected from FMDV clinically suspected cases representing four different governorates in Egypt (Qalubia, Sharkia, Gharbia and Behera). We found that 32 from 45 submitted samples showed positive result in real time RT-PCR and about 26 of these positive samples were isolated on BHK-21 cells giving overt cytopathic effect of the virus. The isolated viruses were identified and serotyped using antigen detection ELISA and RT-PCR that confirmed the three different serotypes A, O and SAT2 with different ratio for their prevalence in Egypt. We found that SAT2 was the predominant circulating serotype in the field, followed by serotype O and serotype A. Hence, this work demonstrates the co-occurrence of three different FMDV serotypes in Egypt. Consequently, further molecular analyses are recommended to confirm these findings to determine the molecular epidemiology of the isolates and to update the nature of future vaccine strains for successful preventive strategies.}, keywords = {FMDV,Real time RT-PCR,isolation,BHK-21 cells,Genetic Characterization}, url = {https://bvmj.journals.ekb.eg/article_116463.html}, eprint = {https://bvmj.journals.ekb.eg/article_116463_1bda6e8dfefb3987de9379765a43d9a9.pdf} } @article { author = {mostafa, ahmed}, title = {Chemical quality of some fresh water fish in Egyptian markets}, journal = {Benha Veterinary Medical Journal}, volume = {39}, number = {1}, pages = {141-145}, year = {2020}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2020.39495.1250}, abstract = {Total ninety random meat samples of fresh fish of Nile tilapia, Bagrus bayad and Clarias gariepinus (thirty of each) were collected from different Egyptian fish markets to be examined for their chemical quality indices to determine their fitness for human consumption. The results showed that the pH value was (6.32±0.01, 6.38±0.02 and 6.29±0.02), TVB-N (mg/100) was (14.54± 0.51, 12.71± 0.43 and 16.76± 0.46), TBA (mg malonaldyde/kg) was (0.82± 0.05, 0.57 ± 0.03 and 0.91± 0.06) and TMA (mg/100) was (6.19± 0.23, 5.49± 0.21 and 6.75± 0.19) in the examined samples of Nile tilapia, Bagrus bayad and Clarias gariepinus, respectively. So, it was appeared that the Clarias gariepinus samples were higher in their pH, TVB-N, TMA, TBA values than other examined samples. All examined samples were fit for human consumption except one Clarias gariepinus sample (3.33%) was unfit due to increasing its pH value than the limit of acceptability. This study shows superiority of Bagrus bayad were more than Nile tilapia followed by Clarias gariepinus samples in their chemical quality indices. So, we recommend proper handling of fish from catching till eating to preserve their human consumption fitness.}, keywords = {Bagrus bayad,pH,TVB-N,tba,TMA}, url = {https://bvmj.journals.ekb.eg/article_116464.html}, eprint = {https://bvmj.journals.ekb.eg/article_116464_ec851a917726aa1f3a6f313dd7ffa2e5.pdf} } @article { author = {Abd-El Hamed, Amira and Kamel, Eman and Atallah, Sanad}, title = {Effect of Calving season and Parity on the Productivity and Profitability of Holstein Friesian Dairy Farms}, journal = {Benha Veterinary Medical Journal}, volume = {39}, number = {1}, pages = {146-153}, year = {2020}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2020.40558.1256}, abstract = {Abstract The aim of this study is to evaluate the effect of calving season and parity on the productivity and profitability of private and governmental dairy farms in Egypt. The data of this study were estimated from 1353 lactation records of Friesian dairy cows. The data were classified according to calving season into winter and summer calvers, and according to parity into six lactation orders from 1st to 6th, then the data were analyzed statistically by using the computer programs SPSS/PC+ version 23. Total milk yield (305MY) and net profit (NP) differed significantly between calving season and parity within production sector, private sector for winter calvers and 3rd parity had the highest 305MY and NP (9759.5Kg - 40960.9EGP&10304.3 kg, 44546.1EGP, respectively), while governmental sector for summer calvers and 6th parity had the lowest 305MY and NP (5028.4Kg-12117.1EGP & 4460.1kg,10027.3EGP, respectively). Total cost (TC) differed significantly within parity and sector not season, it was the highest for private sector at 4th parity (34849.1EGp), while 6th parity for governmental sector had the lowest value (27657.5 EGP). Finally we concluded that winter calvers and 3rd parity achieve the highest profitability for dairy farms.}, keywords = {Key words: Calving season,parity,305My,Economic,Friesian}, url = {https://bvmj.journals.ekb.eg/article_116465.html}, eprint = {https://bvmj.journals.ekb.eg/article_116465_2593cf1a0f333ab000aa0e0ea5a6c47a.pdf} } @article { author = {Abdel-Atty, Nasser and khalafalla, fathy and Barakat, Dina}, title = {Bacteriological Quality of Canned Meat Marketed in Egypt}, journal = {Benha Veterinary Medical Journal}, volume = {39}, number = {1}, pages = {154-158}, year = {2020}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2020.40633.1258}, abstract = {Abstract Because of rapid and busy life style, canned meat are widely consumed in Egypt. Therefore, the main objective of the current study was to evaluate the bacteriological status of canned meat marketed in Beni-Suef city. A total of 150 samples of canned meat represented by canned beef, corned beef, canned chicken sausage, canned chicken luncheon, canned luncheon and canned sausage (25 each) were examined for anaerobic plate count, Staphylococcus aureus count , enterococci count, total Clostridial count and isolation of clostridium perfringens. The highest prevalence of Clostridia were recorded in corned beef and canned sausage (60% each), while their lowest ones were in canned beef and canned poultry sausage (28% each). Twenty, 24%, 16, 12%, 24 and 24% of canned beef, corned beef, canned chicken sausage, canned chicken luncheon, canned luncheon and canned sausage, respectively exceeded the permissible limits of the E.O.S (2005) in relation to staph. aureus count. Enterococci could not be detected from canned chicken luncheon and canned sausage, while the levels of detection in canned beef, corned beef, canned chicken sausage, and canned luncheon were 12, 28, 4, and 12%, respectively. Cl. perfringens failed to be isolated from all the examined samples. It could be concluded that some of the examined samples were exceeding the local and international permissible limits for the examined bacteria, which may reflects under processing or poor storage conditions.}, keywords = {Keywords: Canned meat,canned sausage,Staph. aureus,C.perferingens,enterococci}, url = {https://bvmj.journals.ekb.eg/article_116466.html}, eprint = {https://bvmj.journals.ekb.eg/article_116466_5c549681bee8c5d3030d352864bac888.pdf} } @article { author = {Desouky, Asmaa and Saad, Ahmed and Elshorbagy, Mohamed and Abodalal, Samah}, title = {Isolation and identification of new sub-genotypes of virulent ND virus in broiler chickens in Qalybia governorate}, journal = {Benha Veterinary Medical Journal}, volume = {39}, number = {1}, pages = {159-164}, year = {2020}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2020.32932.1216}, abstract = {A B S T R A C T Newcastle disease (ND) is a highly contagious disease causing huge economic losses to the poultry industry due to high morbidities and mortalities. This study was aimed to isolate and characterize the new sub-genotype NDV strains in Qalybia Governorate during 2017 and 2019. Sixty eight samples were collected from Sixty eight broiler chicken flocks suffering from high mortality reach to 80% with distinctive clinical signs and post-mortem lesions of NDV. NDV strains isolated through inoculation in specific pathogen free (SPF) eggs and were identified by hemagglutination (HA) and hemagglutiation inhibition(HI) techniques. There were 16/20 (about 80%) of selected suspected farm were positive for NDV With HI test . These hemagglutinating agents were confirmed as NDV strains through molecular characterization using one step RT-PCR targeting the partial F-gene of NDV. Sequencing and phylogenetic analysis were successfully performed with eight NDV isolates which possessed the motif 112 R/K-R-Q-K/RR116 at the C-terminus of the F2 protein and F (phenylalanine) at residue 117, indicating that they are velogenic genotype. The present study revealed the circulation of NDV class II genotype VII in Qalybia chicken flocks.}, keywords = {Key words: Newcastle Disease,F protein Cleavage site,genotype VII}, url = {https://bvmj.journals.ekb.eg/article_116467.html}, eprint = {https://bvmj.journals.ekb.eg/article_116467_03e39414fb1bee4ecc9b63b561a0d352.pdf} } @article { author = {Sheir, Suzan and Ibrahim, Hemmat and Hassan, Mohamed and Shawky, Nahla}, title = {Incidence of Psychotropic bacteria in frozen chicken meat products with special reference to Pseudomonas species}, journal = {Benha Veterinary Medical Journal}, volume = {39}, number = {1}, pages = {165-168}, year = {2020}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2020.37744.1238}, abstract = {Total of hundred random samples of frozen chicken products represented by breast, thigh, nuggets and burger (25 of each) were collected from various supermarkets located in Menoufia government. To study the incidence of psychotropic bacteria with special reference to Pseudomonas species. The study revealed that mean values of Psychotropic count were 8.17×103± 1.42×103, 1.95×104± 2.06×104 , 3.63×104± 0.89×104 and 7.58×104± 1.16×104 respectively while the mean value of Psedomonas counts were 3.51×103± 0.76×103 , 6.29×103± 1.12×103,8.44×103± 1.85×103, 1.71×104± 0.36×104 respectively for examined frozen breast, thigh, nuggets and burger. It was obvious that 166 isolates were identified as P. acidovorans, P. aeruginosa, P. alcaligenes, P. cepacia, P. fluorescens, P. fragi, P. proteolytica, P. putida, P. putrefaciens, P. stutzeri, P. vesicularis. The prevalence of Psedomonas were 6, 1, 33, 8, 47, 10, 19, 3, 22, 2 and 15 from examined samples, respectively, where the highest contaminated product was the chicken burger 54/166(32.5%) Pseudomonas isolates and Ps. Fluorescens was the most detected isolate. The isolation of Pseudomonas species from food samples is highly significant Therefore, its presence should be prevented during earlier stages of food preparation. Key words: Psychotropic bacteria, frozen chicken meat products and Pseudomonas species.}, keywords = {Psychotropic bacteria,frozen chicken meat products,Pseudomonas Species}, url = {https://bvmj.journals.ekb.eg/article_116468.html}, eprint = {https://bvmj.journals.ekb.eg/article_116468_82cff13616199a079917fad7a29a4226.pdf} } @article { author = {Abdelaziz, Ibtehal and Abd El-Tawab, Ashraf and Maarouf, Ahmed and Elhofy, Fatma}, title = {Bacteriological and molecular studies on Salmonella isolated from duckling farms at Kaliobia, Egypt}, journal = {Benha Veterinary Medical Journal}, volume = {39}, number = {1}, pages = {169-174}, year = {2020}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2020.38777.1242}, abstract = {This study was conducted on 21 commercial duckling farms (1- 20 days old) inspected to show Salmonella infection in different localities at Kaliobia Governorate. Samples were taken from diseased ducklings and freshly dead for bacteriological examination which resulted in, 94 samples were positive from 630 isolates, where 28 isolates from 33 diseased ducklings and 66 isolates from 72 freshly dead ducklings. Three serogroups of Salmonella were obtained by serological identification (Salmonella Typhimurium, Salmonella Enteritidis and Salmonella Blegdam). The antibiotic sensitivity tests for the isolated strains showed multiple antibiotic resistances (oxytetracycline; amoxicillin; ampicillin; streptomycin; erythromycin and trimethoprim/ sulphamethoxazol) but gentamycin, norfloxacin and ciprofloxacin are the most effective antibiotic on the isolated Salmonella and can be used for treatment of Salmonellosis in duck farms. PCR results appeared that, invA and stn genes were detected in all studied Salmonella isolates;pefA gene was detected in four out of five studied isolates but sefC genewas detected in two isolates only. Finally, isolated Salmonellae are virulant pathogens responsible for disease in ducklings resulting in high mortality and morbidity, gentamycin, norfloxacin and ciprofloxacin are the most proper antibiotics used for treatment of Salmonellosis in duck farms.}, keywords = {duckling,Salmonella,antibiotic resistant,Virulence genes}, url = {https://bvmj.journals.ekb.eg/article_116469.html}, eprint = {https://bvmj.journals.ekb.eg/article_116469_5bb585d1cd1bb97c34a51860998ff767.pdf} } @article { author = {Saad, M. S and Hassan, M. A. and Hassanien, F. S. and Awud, A. A.}, title = {Mycotoxin residues in some fish products.}, journal = {Benha Veterinary Medical Journal}, volume = {39}, number = {1}, pages = {175-179}, year = {2020}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2020.39473.1249}, abstract = {Abstract A grand of 90 random samples represented by smoked herring, canned sardines and frozen fish fillets (30 of each) were collected during their validity period from different areas in Menoufia Governorate to evaluate their mycotoxins quality. It was found that the average values of aflatoxin B1/kg in the examined fish products samples were 51.63 ±4.82µg/kg for smoked herring, 33.14±2.98µg/kg for canned sardine and 19.76 ±2.21µg/kg for frozen fish fillets. The mean values of aflatoxin B2/kg in the examined fish product samples were 37.29±3.75µg/kg for smoked herring, 20.81±3.02µg/kg for canned sardine and 14.57±1.90µg/kg for frozen fish fillets. The average value of aflatoxin G1/kg was 25.06 ±3.18µg/kg for smoked herring, 14.42±1.96µg/kg for canned sardine and 9.65±1.32µg/kg for frozen fish fillets. The average values of aflatoxin G2/kg in the examined fish products samples were 16.22±1.39µg/kg for smoked herring, 11.29±0.92µg/kg for canned sardine and 4.46±0.53µg/kg for frozen fish fillets. Whereas, the mean values of Ochratoxin A/kg were 6.52±0.74µg/kg in the examined smoked herring samples, 5.60±0.61µg/kg in the examined samples of canned sardine and 3.24±0.39µg/kg in frozen fish fillets. Moreover, it was found that aflatoxin B1 was the predominant mycotoxin that detected in the examined fish products samples particularly smoked herring.}, keywords = {Keywords: Aflatoxin,Ochratoxin,smoked herring and fish products}, url = {https://bvmj.journals.ekb.eg/article_116470.html}, eprint = {https://bvmj.journals.ekb.eg/article_116470_0e378e730997983612556097109e9402.pdf} } @article { author = {mohamed, shimaa and Salem, Amany}, title = {Antimicrobial effects of some essential oils and honey on some pathogenic bacteria in chicken meat}, journal = {Benha Veterinary Medical Journal}, volume = {39}, number = {1}, pages = {180-183}, year = {2020}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2020.25900.1190}, abstract = {The present study was designed to investigate antimicrobial activity of garlic (Allium sativum), thyme (Tthymus vulgaris) oils at concentrations (1%) and honey (10%) in chicken meat. A total of 2000g of the fresh chicken meat samples were divided into two groups (A&B) the firist group was inoculated with E. coli (106 cfu/g) and the second one with S. aureus (106 cfu\ g) strains. Both of them were subdivided into untreated (control) and treated ones with garlic (1%), thyme (1%) oils and honey (10%), respectively. All samples were stored at 4°C and subjected to sensory and bacteriological evaluations after 3 hours, 1st, 2nd, 3rd, 4th and 5th day of cold storage. Sensory attributes of different treated chicken meat samples were mostly improved with honey (10%) followed by thyme oil (1%) then garlic oil (1%) when compared with control. Honey (10%) group give the best effectiveness of both pathogens reduction (100%) at 3th, 4th and 5th days with a significant advantage in extend shelf-life of refrigerated chicken meat compared to all groups specially control ones. Generally, some natural compounds proved to be efficient in improve safety and shelf-life. Generally, essential oils & honey proved to be efficient natural preservatives which more safe to human than chemical ones.}, keywords = {Honey,Essential oils,E.coli,S.aureus,Chicken meat}, url = {https://bvmj.journals.ekb.eg/article_116471.html}, eprint = {https://bvmj.journals.ekb.eg/article_116471_e5c45f4ea238edf4ca7fd5a878aaca04.pdf} } @article { author = {mohamed, shimaa and Salem, Amany and Maarouf, Ahmed}, title = {Assessment of some pathogens in retailed poultry meat}, journal = {Benha Veterinary Medical Journal}, volume = {39}, number = {1}, pages = {184-188}, year = {2020}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2020.25898.1189}, abstract = {The present study was performed on 150 random samples of Fresh, half cooked (nuggets) and cooked (grilled) chicken meat (50 of each) were purchased from retail chicken butchers, different supermarkets and restaurants in Kaliobia Governorate, Egypt, to evaluate their bacterial quality and safety. Bacteriological examination of chicken meat samples revealed that, the mean values of APC, Enterobacteriaceae, Coliform, Staphylococcus and S. aureus (cfu /g) were 9.88×105 ±0.61×105; 3.36×10 4±0.26 ×104; 1.43×10 4±0.16 ×104; 2.71×103 ±0.17 ×103 and 2.05×103 ±0.24 ×103 in fresh, 5.48×104±0.23×104; 3.15×102±0.15 ×102; 1.00×102±0.08 ×102; 1.73×102±0.11 ×102 and 1.26×102±0.19 ×102 in half cooked and 4.50×104±0.22×104; 2.06×102 ±0.13 ×102; 1.09×102 ±0.10 ×102; 1.05×102 ±0.09 ×102 and 1.02×102 ±0.15 ×102 cooked chicken meat samples respectively. Moreover, the incidence of E. coli were 14% in fresh O55: H7(6%); O125: H21(4%); O111: H2 (2%) and O146: H21(2%); 6% in half cooked O55: H7(2%); O125: H21(2%) and O111: H2(2%) and 6% in cooked chicken meat samples O55: H7(4%) and O111: H2(2%). Meanwhile, the incidence of Coagulase positive S. aureus were (38%) (26%) and (20%) in fresh, half cooked and cooked chicken meat samples. According to SET- RPLA test, 7 strains out of 12 examined strains were enterotoxigenic. In addition, PCR showed that S. aureus enterotoxins sea, seb, sec, sed, see were detected in 10 S.aureus isolate from samples.}, keywords = {Poultry meat,PCR,Food poisoning,SET- RPLA test}, url = {https://bvmj.journals.ekb.eg/article_116472.html}, eprint = {https://bvmj.journals.ekb.eg/article_116472_063ad04f81530c54dcd8706e0a6a324a.pdf} } @article { author = {Soror, Eman}, title = {Effect of Flavomycin and Synbiotic on experimentally-infected common carp with Pseudomonas aeruginosa}, journal = {Benha Veterinary Medical Journal}, volume = {39}, number = {1}, pages = {189-193}, year = {2020}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2020.20660.1173}, abstract = {This study investigated the antibacterial effect of flavomycin and synbiotic against Pseudomonas aeruginosa (p. aeruginosa) beside the weight gain and hemato-biochemical changes in common carp. Apparently healthy 150 common carp weighing 110 ± 10 g were divided into six equal groups. The 1st group received basal diet without any additives (control group). The 2nd group received basal diet with 8 mg/kg diet flavomycin for 30 days. The 3rd group received basal diet with 3 mg/kg diet synbiotic for 30 days. Fish in 4th, 5th and 6th groups were experimentally infected with P. aeruginosa. The 4th group was left untreated. The5th and 6th groups were treated with 8 mg/kg diet flavomycin or 3 mg/kg diet symbiotic, respectively for 5 days. Swabs were collected to reisolate p. aeruginosa. Blood samples were collected for hemato-biochemical analysis. Healthy fish received flavomycin or synbiotic revealed significant increase in body weight gain, RBCs, Hb, PCV with non-significant increase in total protein, albumin, globulin, A/G ratio, AST, ALT, urea, creatinine. Infected fish with P. aeruginosa showed tail and fin rot, hemorrhagic ulcerative skin, superficial ulcers beside mortality rate up to 32% with significant decrease in body weight, RBCs, HB, PCV %, total protein, albumin, globulin and significant elevation in AST, ALT, ALP, urea and creatinine. Infected fish treated with flavomycin or synbiotic improved the clinical signs, reduced mortality rate to 8% and 12%, respectively, and decreased rate of infection. In conclusion, flavomycin or synbiotic has effective antibacterial effect against P. aeruginosa.}, keywords = {common carb,Flavomycin,hematobiochemical,Pseudomonas aeruginosa,synbiotic}, url = {https://bvmj.journals.ekb.eg/article_116473.html}, eprint = {https://bvmj.journals.ekb.eg/article_116473_9247e3ec6ba7c236032f410dba893146.pdf} }