@article { author = {El-Seadawy, Islam and Hussein, Samy and El-Senosy, Yakout and El-Nattat, Walid and El-Tohamy, Magda and Hussein, Ahmed}, title = {Effect of pomegranate peel methanolic extract on oxidative/antioxidant status of chilled diluted rabbit semen}, journal = {Benha Veterinary Medical Journal}, volume = {33}, number = {2}, pages = {1-8}, year = {2017}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2017.29982}, abstract = {The present study aimed to assess the lipid peroxidation and antioxidant enzymes activities of diluted rabbit semenenriched with different concentrations of pomegranate peel methanolic extract (PPME) during chilled storage. Semenwas collected from 10 rabbit bucks, pooled, then divided into five aliquots and diluted each in 5 ml Tris-citric acidglucose-egg yolk extender (TCGY). The 1st aliquot served as control, while PPME was added in different concentrations(1.6, 2.0, 2.4 and 2.8 mg/5 ml TCGY extender) to the aliquots 2, 3, 4 and 5, respectively. Diluted semen samples wererefrigerated at 5°C for 72 hours. Lipid peroxidation (MDA), H2O2 concentration, SOD, CAT activities and GSHconcentration were evaluated in chilled seminal plasma during the chilling period. Obtained results clearly demonstratedthat the addition of 1.6 – 2.8 mg PPME in the chilled seminal plasma proved to be beneficial for minimizing MDA andH2O2 concentrations; in addition, they increased SOD, CAT activities and GSH concentration compared to the control.While, the concentrations of 2.4 and 2.8 mg were the best PPME enrichments that maintained the highest SOD, CATactivities and GSH concentration. It was concluded that the enrichment of rabbit semen TCGY extender with 2.4 – 2.8mg PPME during chilling had significantly decreased sperm lipid peroxidation and H2O2 production beside they enhancedthe activities of antioxidant enzymes and the reduced glutathione.}, keywords = {Male rabbits,semen extender,Pomegranate peel extract,antioxidants}, url = {https://bvmj.journals.ekb.eg/article_29982.html}, eprint = {https://bvmj.journals.ekb.eg/article_29982_114b42b25c301c19410e4052e942b1d5.pdf} } @article { author = {Hussein, Samy and EL Senosi, Yakout and El Barky, Amira and Al Fakharany, Mohamed}, title = {Biochemical evaluation of the combination of Pegylated IFN-α2b and Ribavarin therapy of Egyptian patients suffering from Hepatitis C Virus}, journal = {Benha Veterinary Medical Journal}, volume = {33}, number = {2}, pages = {9-16}, year = {2017}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2017.29983}, abstract = {The purpose of this study was to evaluate the biochemical parameters and cytokines levels in infected Egyptian patient.This study was conducted on 32 patients. 25 of them were suffering from chronic HCV and the other 7 males are selfreportedhealthy volunteers. They were classified as the following: Group I (control healthy group), Group II (HCV-nontreated group) Group III- Group VI (HCV-treated with 12,24,36,48 ampoule of interferon). The obtained results showeda significant increase in the activities of serum liver marker enzymes ALT, AST and ALP, cytokines Il2, Il4 and Il10,also a significant increase was observed in serum Ferritin and Alpha feto protein. On the other hand, a significantdecrease in both sera total protein and albumin level. Whereas administration of combination of Pegylated IFN-α2b andRibavarin significantly improved the alert in the biochemical parameters. These results suggested that, combinationtherapy of both peg-interferon and ribavirin, emphasizing the influence of these compounds for patient with HCV,possibly preventing alteration of enzymes of liver activities. Because peg-interferon treatment can reduce liver injuryand anti-inflammatory effect, as well as by decreasing plasma cytokine as cytokines, is present in patients with HCVliver disease. Furthermore, treatment with IFN-α diminishes the Th2 cytokine response. Thus, modulation of T- cellfunction and cytokine production may be one mechanism whereby IFN-α therapy results in reduced viral burden}, keywords = {Hepatitis C virus,Cytokines,TNF-α,IgG and total protein}, url = {https://bvmj.journals.ekb.eg/article_29983.html}, eprint = {https://bvmj.journals.ekb.eg/article_29983_43676296df00a79f00af1b14c8a7fc6d.pdf} } @article { author = {Abd El- Tawab, Ashraf and M., Nabih and Agag, Mohsen and A., Al-Abbou}, title = {Bacteriological and molecular studies on Shiga-Toxin producing Escherichia coli causing cattle clinical mastitis}, journal = {Benha Veterinary Medical Journal}, volume = {33}, number = {2}, pages = {17-26}, year = {2017}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2017.29984}, abstract = {A total of 194 milk samples from clinically mastitis cattle cows were collected from Giza, Monofia, Fayoum, Ismailia,and Beni-Suef Governorates. All samples were collected during the period from December 2016 till June 2017.Bacteriological study gave a total of 29 positive strains of Escherichia coli (E.coli) in the rate of (14.9%) from all collectedsamples. Twelve E.coli isolates were identified from cultured samples in a single manner (6.2%) and was isolated withStaphylococcus aureus (S.aureus) by 8/194(4.1%), with Streptococcus species (Strept. spp) by 3/194(1.57%), meanwhileit was isolated with S.aureus and Strept. spp. by 6/194(3.1%). On the other hand 18 clinical mastitic milk samples wereshowed no growth of any pathogenic microorganisms on ordinary and specific used media of bacteriology from allinvestigated milk samples by (9.3%). It was observed that several serotypes were recovered from clinical cases of milksample with different E.coli infection as O27, O146, O125, O126, O111, O20, and O157. Concerning the sensitivity testto choice the suitable antibacterial drug(s) for treatment clinical mastitis in cattle cows the data revealed that ,Cefiquinom,Gentamycin and Amoxicillin +Clavulinic acid were the antibacterial drugs of first choice that could be used to overcomea great number of single isolated E.coli causing clinical mastitis. Vice versa, the resistant antibiotics for single E.coliinfection causing clinical mastitis were Amoxicillin, Ampicillin, and Neomycin. Studied strains were gave a positiveresults for virulence E.coli genes: phoA ,ompA and fimH in 5 examined strains (100 %),classified as follow :(two strainsof O27/28.6%) ,( one strain of O125/14.3%) ,( one strain of O126/14.3%) , and (one strain of O146/14.3% ). while Stx1and Stx2 virulence genes were detected in only 2 studied strains of E.coli in a total percentage of 28.6 %, divided into,O111(14.3%) and O157(14.3%).}, keywords = {Cattle diseases,Clinical mastitis,E.coli infection,Molecular biology,Virulence genes,bacterial Antibiogram,Egypt Governorates}, url = {https://bvmj.journals.ekb.eg/article_29984.html}, eprint = {https://bvmj.journals.ekb.eg/article_29984_cb6dd8d1d624ff1d30cfedc7475a8fb0.pdf} } @article { author = {Abd El- Tawab, Ashraf and Nabih, Ashraf and Agag, Mohsen and Abd Ali., Marwah}, title = {Molecular studies of virulence genes of Salmonella Typhimurium causing Clinical mastitis in dairy cattle}, journal = {Benha Veterinary Medical Journal}, volume = {33}, number = {2}, pages = {27-37}, year = {2017}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2017.29985}, abstract = {A total of 213 milk samples from clinically mastitic cattle cows were collected from different Governorates of Egypt andtransferred in ice box as soon as possible to Bacteriological lab. in Animal Reproduction Research Institute (ARRI) inGiza Governorate (AL-Haram) for bacteriological examination of most important pathogens causing clinical mastitis withspecial references for isolation and strict identification of Salmonella species. All samples were collected during theperiod from December 2016 till July 2017 from governorates of Egypt. The bacteriological investigations revealed that 8(3.7%) of Salmonella isolates were identified biochemically from all examined samples. Serological study showed that atotal of 5 (2.3%) of Salmonella isolates were typed as Salmonella Typhimurium. Two strains of Salmonella Typhimuriumwere isolated singly in the rate of (0.93%) from all examined samples, also another two strains of SalmonellaTyphimurium were isolated mixed with Staph aureus in the rate of (0.93%), meanwhile only one strain of same specieswas isolated mixed with E.coli in the rate of (0.47%). Cefiquinom and Enrofloxacin were sensitively in the rate of (100%),Ampicillin, Chloraphincol, Cloxacillin were resistance in the rate of (100 %) to Streptomycin and Amoxicillin. Themolecular examination confirmed that all 5 examined serotyped strains were Salmonella Typhimurium. Virulence genesinvA, hilA, avrA, were detected in examined sample by 100%, meanwhile sopE, ssaQ, and fimH genes were not detectedby zero%. The objectives of the present study was to investigate the occurrence of Salmonella serotypes as well as todetermine the frequency distribution of Six virulence genes (invA, hilA, avrA, ssaQ, sopE and fimH) in salmonellaisolates from cattle clinical mastitic milk, in addition to determine the drugs of choice for treatment of most Salmonellastrains causing cattle clinical mastitis.}, keywords = {Cattle diseases- Clinical mastitis–Salmonella Infection-Molecular study- Virulence genes}, url = {https://bvmj.journals.ekb.eg/article_29985.html}, eprint = {https://bvmj.journals.ekb.eg/article_29985_14d3aa2d0220ef8517c0716ed51ba331.pdf} } @article { author = {Abd El- Tawab, Ashraf and Hassan, Wafaa and AL-Siraj, Suhair}, title = {Bacteriological and molecular studies of Staphylococcus. aureus isolated from foods and human contact}, journal = {Benha Veterinary Medical Journal}, volume = {33}, number = {2}, pages = {38-45}, year = {2017}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2017.29986}, abstract = {A total of 50 samples were collected from different food (meat product, chicken, sandwiches, pasta, Broth, fish,Appetizer) and human contact (hand). Samples were examined microbiologically for the presence of Staphylococcusaureus was isolated from the samples in a ratio present (8) and identified by biochemical identification. Staph. aureusstrains were tested for antimicrobial sensitivity and all strains showed a 100% resistant to ampicillin. The resistance tooxacillin, amoxicillin, trimethoprim, gentamicin and tetracycline was in a different ratio. However, All the strains weresensitive to levofloxacin and ciprofloxacin. Using PCR technique, amplification of some virulence gene as (tst,icaD, sea)and antibiotic resistance genes (mecA , blaZ , vanA) was performed from the extracted DNA of Staph. aureus strains.All extracted DNA samples of Staph. aureus showed a positive results for mecA ,tst , blaZ, icaD and sea genes .However, all the samples did not give any PCR product on agrasoe gel. Using sequencing technique gene in two positivestrains, the Phylogenetic analysis of mecA gene of these isolates were clustered together and little away from otherpublished isolates of MRSA, Amino acid identities were 99% which had accession number MF774211 correspondingGenBank sequence.}, keywords = {Staphylococcus. aureus,mecA,blaZ,vanA,tst,icoD and sea genes,antibiotic sensitive,sequences mecA gene,human,foods}, url = {https://bvmj.journals.ekb.eg/article_29986.html}, eprint = {https://bvmj.journals.ekb.eg/article_29986_47ab92007704e50b68ae01a7584cf8b2.pdf} } @article { author = {El-senosi, Yakuot and A., MoKhtar and El komy, Ashraf}, title = {Biochemical studies on the effect of curcumin in experimentally induced osteoarthritis in rats}, journal = {Benha Veterinary Medical Journal}, volume = {33}, number = {2}, pages = {46-51}, year = {2017}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2017.29987}, abstract = {Osteoarthritis (OA) is the most common disease of joints in adults around the world. Osteoarthritis was induced in ratsby intra-articular injection of 2 mg/kg b.wt of monosodium-iodoacetate (MIA). Thirty six male, albino rats were dividedinto four equal groups. 1st group was not subjected to any intervention and was used as normal control. 2nd group (OA -induced group) received injection of MIA intra-articular. 3rd group ( OA+ curcumin treated group) injected MIA andtreated with 200 mg/kg b.wt of curcumin orally and daily / 28 day. 4th group ( curcumin normal group) received 200mg/kg b.wt of curcumin orally and daily / 28 day. Blood samples were collected at 28th day. The obtained results showeda significant increase in MDA level and SOD activity in MIA induced OA. On the other hand, a significant decrease inGSH, GPx, CAT and GAG were observed in OA induced rats. Curcumin treatment in OA induced rats significantlyincreased GSH concentration , GPx, CAT and GAG activities with marked decrease in MDA concentration. Inconclusion, the present study demonstrated that curcumin administration provided an effective protection and treatmentagainst OA and oxidative damage in MIA injected rats.}, keywords = {OA,Curcumin,Inflammatory mediators,oxidative damage}, url = {https://bvmj.journals.ekb.eg/article_29987.html}, eprint = {https://bvmj.journals.ekb.eg/article_29987_f17f16f741ddc0ee9e7db09c1c627597.pdf} } @article { author = {El-Senosi, Yakout and Atwa, Hend and Prince, Abdelbary and Afify, Adel}, title = {Development of Simple Multiplex Real-Time PCR Assays for Some Probiotics Detection}, journal = {Benha Veterinary Medical Journal}, volume = {33}, number = {2}, pages = {52-57}, year = {2017}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2017.29988}, abstract = {The main objective of this study to investigate the development of simple multiplex real time for some probioticsdetection. The probiotics defined as Live microorganisms when administered in adequate amounts confer a health benefiton the host. Most commonly used probiotics are Lactic acid bacteria (LAB) and bifido bacteria. There are other examplesof species used as probiotics (certain yeasts and bacilli). Probiotic supplements are popular now a day. From the beginningof 2000, research on them has increased remarkably. The beneficial effects of probiotics are now day’s widely studied intreatment of many prevailing diseases. In this study, we present the development of molecular methods. Conventionalmultiplex PCR and SYBR Green based real time PCR assays were performed using genus and species specific primers.}, keywords = {probiotics,Lactic acid bacteria (LAB),Bifido bacteria,Conventional multiplex PCR,SYBR Green Real time PCR}, url = {https://bvmj.journals.ekb.eg/article_29988.html}, eprint = {https://bvmj.journals.ekb.eg/article_29988_6280bf9ac2f90a481813d840755953be.pdf} } @article { author = {A., El-Sayed, and A., El-Komy and Abd Al-Wahed, Amany}, title = {Protective Effect of Curcumin Against Adverse Effects of Meloxicam in Rats}, journal = {Benha Veterinary Medical Journal}, volume = {33}, number = {2}, pages = {58-64}, year = {2017}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2017.29989}, abstract = {The aim of the present study was to determine the adverse effects of meloxicam on lipid profile, glucose profile andhematology as well as the protective effect of curcumin in rats. Thirty-six male Wister albino rats were randomly dividedinto main four groups; Group (1): control (administered saline only 0.5 ml); Group (2): meloxicam was administeredintraperitoneally at level of 5.8 mg/kg body weight for 15 days; Group (3): curcumin extract was administered orally atlevel of 200 mg/kg body weight for 15 days; Group (4): were administered meloxicam intraperitoneally with oraladministration of curcumin extract for 15 days. The findings revealed that there were significant increase in serumcholesterol level and serum triglycerides level of rat given meloxicam. There were significant decrease in serumcholesterol level and serum triglycerides of rats administered meloxicam with curcumin group. There was a significantincrease in serum glucose level of rat given meloxicam. There was significant decrease in serum glucose level ofmeloxicam with curcumin group compared to meloxicam group. There was alteration in hematological parameters of ratgiven meloxicam. On the contrary, there was an improvement in hematological tests of meloxicam with curcumin groupand improvement of histopathological study compared to meloxicam. On conclusion, administration of meloxicamintraperitoneally 5.8 mg/kg body weight resulted in impairment in lipid profile, glucose profile and hematologicalparameters and these impairments could be improved by curcumin administration.}, keywords = {meloxicam,Curcumin,Lipid profile,glucose profile,blood pictures}, url = {https://bvmj.journals.ekb.eg/article_29989.html}, eprint = {https://bvmj.journals.ekb.eg/article_29989_2abd9b287312d32976ade8021325bf6e.pdf} } @article { author = {Gaballah, Mahmoud and El-Mashad, Abd- Elbasat and Khater, Dalia and Zayed, Rasha}, title = {Pathological study on urea utilization by poultry}, journal = {Benha Veterinary Medical Journal}, volume = {33}, number = {2}, pages = {65-74}, year = {2017}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2017.29994}, abstract = {The present work aimed to investigate the histopathological aspects, growth performance, urea residue in muscle and liver of broilerbirds and chemical composition of the breast muscles in birds fed graded levels of urea –treated ration. 80, day old chicks werereared for 6 weeks. After one week of age, chicks were randomly divided into 4 equal groups fed on 1.25%, 2.5%, 5% and 0% ureain their ration. Five birds from each group were sacrificed at the day 7, 14, 28 and 35 from adding urea. The most clinical signswere respiratory symptoms, watery diarrhea and staggering gait. Body weight and feed consumption were increased in group I &II, while the group III showed mild decrease. Urea residue in muscle and liver increased significantly in group III when comparingwith other groups. The chemical composition of the muscle showed that the moisture content in group III was high thangroup IV. There was non-significant increase in the crude protein level in group II. There was significant increase in ashpercent in group II. The fat content in the muscle of all urea treated groups was significant decrease than IV group. Grossly,kidneys and the liver were severe enlarged with pale anemic and mottled coloration. Lungs and the muscles were severe congestedand dark brownish color with petechial and linear hemorrhage in muscle. The brain showed mild hyperemia. Histopathologicalexamination in the kidneys showed degenerative changes in the epithelial cell lining of the renal tubules. The glomeruli showedshrinkages of their glomerular tuft and hyalinization of the Bowman's capsules. Liver showed focal areas of necrosis in the hepaticparenchyma. There was severe thickening of the hepatic capsule. The bile duct showed hyperplasia of their epithelial cell liningwith the presence of desquamated epithelia in the ductile lumen with newly formed bile ductile. Most of hepatic and pulmonaryblood vessels showed thrombosis. Lungs and muscle and brain showed congestion of the blood vessels. The bronchioles showedcatarrhal bronchopneumonia. There was osseous metaplasia among the pulmonary tissue. The neuron was suffering fromTygrloysis with presence of satellotosis and neurophagia. The intestine showed degenerative changes. Myomalecia of some musclecell and suffering from mild degree of hyaline degeneration. It is recommended that lower inclusion levels of urea diets (1.25%)rather than the 2.5% and 5% used in this study be employed for a short period of time.}, keywords = {urea –residue –histopathological –broiler birds}, url = {https://bvmj.journals.ekb.eg/article_29994.html}, eprint = {https://bvmj.journals.ekb.eg/article_29994_fe8fc5e671143e40513938ff5910001d.pdf} } @article { author = {AL-Sharkawy, Alaa and Gab-Allah, Mahmoud and El-Mashad, Abdel-Baset and Khater, Dalia}, title = {Pathological study on the effect of some food additives in male albino rats}, journal = {Benha Veterinary Medical Journal}, volume = {33}, number = {2}, pages = {75-87}, year = {2017}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2017.29996}, abstract = {The histo and clinicopathological effect of MonoSodium Glutamate (food enhancer) and Sodium MetaBisulfite(food preservatives) on different tissues and organs of male Albino rats were investigated. Eighty male Albinorats (150-180 gm) were divided into 5 groups: control group and 4 groups administrated 30 and 60 mg MSG/kg b.wt; 260 and 520 mg SMB /kg b.wt for 12 weeks per os. Rats were sacrificed at 2, 4, 8 and 12 weeks frombeginning the experiment. Significant decrease in Hb concentration, PCV, MCV and MCH; and significantincrease in RBCs, leukogram, platelet counts, GPT, GOT, BUN, creatinine and total protein were observed inrats administred 30 and 60 mg MSG /kg b.wt. Histopathologically, mild changes were observed in liver,kidney, heart, stomach and intestine. Multifocally, marked degenerative and necrotic changes were seen in thelining epithelium of the seminiferous tubules and neurons particularly in high doses after 8 and 12 weeks postMSG administration. On the other hand, administration of SMB by a dose 260 and 520 mg/kg b.wt resulted insignificant increase in Hb, MCH, MCHC, Platelets, total leukocytic counts, GPT, GOT, albumin, BUN andcreatinine values. Microscopically, hyperplasia of the biliary epithelium, lymphoid depletion of white pulpeof the spleen and mild degenerative changes in the lining epithelium of the seminiferous tubules, neurons andcardiac muscle were detected. Moderate changes were seen in the renal tissue characterized by degenerativechanges in the endothelial cells of glomeruli and renal tubular epithelium with desquamation of the liningepithelial cells and presence of hyaline casts in the lumen. Multifocally, necrotic gastroenteritis with heavyleukocytic infiltration in the lamina propria of the stomach and intestine. In conclusion, the addition of MSGresulted in marked changes in brain and testes while, SMB lead to marked changes in gastrointestinal tract andbrain. These changes are dose and time independent in MSG and dose independent only in SMB.}, keywords = {MSG,SMB,Food enhancer,Food preservatives}, url = {https://bvmj.journals.ekb.eg/article_29996.html}, eprint = {https://bvmj.journals.ekb.eg/article_29996_0cd1e1f9957a9a722c69170181af4deb.pdf} } @article { author = {Gab-Allah, Mahmoud and El-Mashad, Abdel-Baset and Ahmed, Sayed and Badawy, Sara}, title = {Experimental pathological studies on the ability of Stem cells for regeneration of hepatocytes in rats}, journal = {Benha Veterinary Medical Journal}, volume = {33}, number = {2}, pages = {88-98}, year = {2017}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2017.29998}, abstract = {Stem cell therapy is an important catigory of regenerative medicine has the potential to provide a valuable adjunct andalternative to liver transplantation with treatment of liver fibrosis and cirrhosis. In this approch, 70 male albino rats wererandomly divided into 4 groups: Group 1: Control group (20 rats), Group 2: TAA-treated group (20 rats), Group 3:Mesenchymal stem cells treated group (15 rats) and Group 4: TAA- Mesenchymal stem cell –treated group (15 rats). Ourresults showed that TAA is significantly increase the activities of ALT, AST, and ALP and reduced the levels of totalprotein, albumin and globulins in the serum. Moreover, TAA successfully induced liver cirrhosis characterized by coarsenodular liver surface with severe fibrous connective tissue proliferation led to pseudolobulation of hepatic parenchyma.Meanwhile, after treatment with Mesenchymal stem cells (MSCs) the liver of the rats was grossly nearly normal andmicroscopically thin strands of fibrous connective tissue in between the hepatic lobules with mild degenerative andnecrotic changes and significant improvement in the liver blood parameters. These findings concluded that MSCs haveameliorative effect on chemically induced liver cirrhosis through their regenerative capacity and antifibrotic effect.}, keywords = {Liver cirrhosis,TAA,Antifibrotic,MSCs}, url = {https://bvmj.journals.ekb.eg/article_29998.html}, eprint = {https://bvmj.journals.ekb.eg/article_29998_f46e318ff1943cf60409c4f314864648.pdf} } @article { author = {Abou Zaid, Omayma and Ahmed, Fatma and Badwi, Abdel Fatah and Ibrahim, Naglaa}, title = {Effect of natural nanoparticles products on hepato-renal functions in breast carcinoma female rats}, journal = {Benha Veterinary Medical Journal}, volume = {33}, number = {2}, pages = {99-106}, year = {2017}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2017.30003}, abstract = {The aim of this study was to evaluate the effect of natural nano-composite products in combination with doxorubicinchemotherapy treatment on serum biochemical profile in mammary cancer induced in fifty female rats divided asfollowing; control normal n= 10, mammary carcinogenesis Induced group with MNU (50 mg/ kg. b. wt. / I.P dose) n=40which divided into three subgroups (10 rats in each group): mammary carcinoma non-treated gp, doxorubicin treatedgroup (33 mg /kg. b .wt. / I.P. dose), basic nano-curcumin (35 mg/ kg. b. wt. /oral dose) + doxorubicin treated group andbasic nano-brown algae (70 mg /kg. b .wt. / oral dose) + doxorubicin treated group. Blood urea nitrogen (BUN) and serumcreatinine level, AST and ALT activities were determined during the course of chemotherapy treatment among differentgroups. The level of BUN, creatinine and AST activity were more than normal reference range in doxorubicinchemotherapy treatment, basic nano-curcumin and basic nano-brown algae; while the value of serum ALT was reportedto be within the normal reference range in case of treatment by using basic nano-curcumin with doxorubicinchemotherapy. In conclusion, the obtained results suggest that, treatment with chemotherapy and basic nano-curcumin incombination improve serum biochemical profile in breast carcinoma induced in rats.}, keywords = {breast cancer,biochemical profile,Chemotherapy,BUN,creatinine,AST,ALT}, url = {https://bvmj.journals.ekb.eg/article_30003.html}, eprint = {https://bvmj.journals.ekb.eg/article_30003_ac5ee0d34158e43060c0d41f2573d81a.pdf} } @article { author = {Abou Zaid, Omayma and Ahmed, Fatma and Badwi, Abdel Fatah and Ibrahim, Naglaa}, title = {Protective role of new nanocomposite natural product (basic nano-curcumin) against breast carcinoma}, journal = {Benha Veterinary Medical Journal}, volume = {33}, number = {2}, pages = {107-116}, year = {2017}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2017.30006}, abstract = {Present study focused on evaluation of the effect of national nancomposite product on serum biochemical profile andprogrammed cell death (apoptosis) by using Annexin V-FITC and PI test of breast carcinoma female rats. Basic nanocurcuminwas tested for its protective effect on breast cancer female rats (n= 30) divided in two main groups (NTBR) and(TBR) which in turn classified into (TBR- Control Positive and TBR- basic nano- curcumin protective groups). Basicnano-curcumin was formulated and tested for both IC50 (IC50 = 1.0 μg) and LD5o (35 mg/kg by wt.) by using in vitro andin vivo techniques, respectively. Breast cells apoptosis was determined by Annexin V-FITC, which is a fluorescent probebinds to phosphotidylserine (PS) in a Ca 2+ dependent pathway. Breast Cells were stained with Annexin V and PI. Theapoptotic cells [Annexin (+) and PI (-)] were differentiated from necrotic cells [Annexin (-) and PI (+)] using flowcytometry technique. The anti-proliferative effect of basic nano-curcumin appeared much earlier due to its nano form.Also, Liver [ALT (40U/l), AST (85 U/l)] and kidney [BUN (19 mg/dl), Creatinine (0.5 mg/dl)] functions were decreasedwithin normal ranges}, keywords = {Basic nano-curcumin,Annexin,BUN,creatinine,AST,ALT}, url = {https://bvmj.journals.ekb.eg/article_30006.html}, eprint = {https://bvmj.journals.ekb.eg/article_30006_388c770765014ea18dbf02312f98c30b.pdf} } @article { author = {Ibrahim, Hemmat and Amin, Reham and Eleiwa, Nesreen and Rezk, Heba}, title = {Antibacterial Action of Zinc Oxide Nanoparticles against Staphylococcus aureus in Broiler Breast Fillet}, journal = {Benha Veterinary Medical Journal}, volume = {33}, number = {2}, pages = {117-122}, year = {2017}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2017.30009}, abstract = {Zinc oxide can be called a multifunctional material thanks to its unique physical and chemical properties. Practicalapplication of zinc oxide nanoparticles (ZnO NPs) suspension with different concentrations (5, 8, and 10 mM) wereinvestigated against Staphylococcus aureus artificially inoculated into broiler breast fillets. The results indicated that ZnOsuspensions (5, 8 and 10 mM) had a significant inhibitory effect on the growth of Staphylococcus aureus during 12 daysof refrigerator storage at 4ºC. Accurately, ZnO NPs 10 mM showed the highest reduction percentage (25.35%) toStaphylococcus aureus from 8.6 to 6.42 log cfu/g, compared to other concentrations (5 and 8 mM). Thus, that theantibacterial activity of ZnO was concentration dependent.}, keywords = {ZnO nanoparticles,broiler breast fillets,Antibacterial Activity-Staphylococcus aureus}, url = {https://bvmj.journals.ekb.eg/article_30009.html}, eprint = {https://bvmj.journals.ekb.eg/article_30009_52475423a3f18a3484a839236c35fd71.pdf} } @article { author = {S., Amani and N., Shaimaa and K., Mohamed}, title = {Challenge of multi drug resistant stx1 harboring E. Coli in meat and fast foods}, journal = {Benha Veterinary Medical Journal}, volume = {33}, number = {2}, pages = {123-128}, year = {2017}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2017.30011}, abstract = {A total of 120 random samples of fresh (30) and processed (90) beef and poultry meat products were collected fromdifferent supermarkets in Menoufia governorates .these samples were examined for the presence of multi –drug resistantStx1 harboring E. Coli, the obtained results revealed that the highest incidence of E.COLI in processed samples wererecorded in chicken fahita (46.66 %), while beef burger showed the lowest incidence(20%),but the highest incidence infresh samples were recorded in chicken thigh(40%),on the other hand, chicken breast and fresh beef were the sameincidence (20%).The illustrated results showed that the serovars O2:H6 producing Stx1,while the serovarsO91:H21,O86,O153:H2 and O44:H18 which isolated from different products producing Stx2, these serovars showedmulti-drug resistance to major group of antimicrobials.}, keywords = {meat products,E. coli,stx1,Antibiotic resistance}, url = {https://bvmj.journals.ekb.eg/article_30011.html}, eprint = {https://bvmj.journals.ekb.eg/article_30011_c29bcb9e6393b96890560ea26b48c2b5.pdf} } @article { author = {Abdel-Gawad, M. and Azab, M. and Ismail, R. and Nafeaa, A. and A. El-Magd, M.}, title = {The Action Of Myo-Pro gene On The Morphology Of Skeletal Muscle Of Fayoumi Chicken}, journal = {Benha Veterinary Medical Journal}, volume = {33}, number = {2}, pages = {129-136}, year = {2017}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2017.30013}, abstract = {The aim of the present study was to evaluate the changes in skeletal muscle which may occur after in ovo injection of Myo-Pro gene . A total number of 200 Fayoumi fertilized pure breed eggs were obtained from EL-Tkamoly poultry project - EL-Azab – Fayoum and divided into two equal groups. Control group, n= 100 that was injected with saline, and treated group n = 100 was injected with MyoPro gene. To check both possibilities, we examined the gastrocnemius muscle fibers number and diameter (cross sectional area). The results revealed that Chicken hatched from Myo-Pro injected eggs showed a significant increased muscle masses. This increase in muscle mass and subsequently meat production is due to hypertrophy rather than hyperplasia. From the obtained results, using Myo-Pro gene causing improvement in skeletal muscle fiber (diameter and number) in Fayoumi chicken.}, keywords = {Fayoumi chicken,Eggs,Myo-Pro,skeletal muscle}, url = {https://bvmj.journals.ekb.eg/article_30013.html}, eprint = {https://bvmj.journals.ekb.eg/article_30013_3bca8b8a9d01b7ecfd019536049d32a7.pdf} } @article { author = {Abdel-Gawad, M. and Azab, M. and Ismail, R. and Nafeaa, A. and A. El-Magd, M.}, title = {Effect of in Ovo Injection of MyoPro Gene on Growth Parameter in Fayoumi Chicken}, journal = {Benha Veterinary Medical Journal}, volume = {33}, number = {2}, pages = {137-148}, year = {2017}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2017.30015}, abstract = {The current study aimed at evaluation the effects of in ovo injection of the myostatin inhibitor, myostatinpro-peptide (MyoPro) gene on growth performance of Fayoumi chicken. A total number of 200 Fayoumifertilized pure breed eggs were obtained from EL-Tkamoly poultry project - EL-Azab – Fayoum. Eggs weredivided into two equal groups. Group 1(control group, n=100) was injected with saline, and group 2 (treatedgroup, n=100) injected with Myo-pro gene. Growth parameters were recorded (body weight, body weightgain, feed consumption per group was measured for calculating feed conversion ratio). The obtained resultsrevealed that injection of myo-pro to Fayoumi eggs resulted in highly significant increase in body weight andweight gain during all experimental period when compared with control group. Feed conversion ratio ofMyo-pro injected group was significantly lower than control group. However, food intake showed nosignificant change between the two groups. From the obtained results, injection of Myo-Pro gene causingmarked increase in body weight gain and improvement in feed conversion ratio without change in foodintake in fayoumi chicken.}, keywords = {Fayoumi chicken,in Ovo-adminstration,MyoPro,body weight}, url = {https://bvmj.journals.ekb.eg/article_30015.html}, eprint = {https://bvmj.journals.ekb.eg/article_30015_6dbf1bb3eb331b36606e21a9c5b1075e.pdf} } @article { author = {Hussein, Samy and Abdel Aal, Samir and Ismail, Hany}, title = {Neurodegeneration and oxidative stress induced by tramadol administration in male rats: The effect of its withdrawal}, journal = {Benha Veterinary Medical Journal}, volume = {33}, number = {2}, pages = {149-159}, year = {2017}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2017.30017}, abstract = {The purpose of this study was to evaluate the possible neurodegeneration and oxidative stress induced bytramadol administration in male rats. One hundred male albino rats were divided into three groups. Group I(normal control): consisted of twenty rats, administered saline solution. Group II (therapeutic dose gp): fortyrats, received oral dose of tramadol HCl suspended in saline solution equivalent to 22.5 mg/kg/day for nineweeks; ten rats were left for further two weeks without any additional treatment as a recovery period. GroupIII (over- dose gp): forty rats, received oral dose of tramadol HCl suspended in saline solution at doses of 30,60 and 90 mg/kg/day on the first, the second and the third three weeks of the study, respectively; ten ratswere left from Group III for further two weeks without any additional treatment as a recovery period. Theobtained results showed significant increase in serum liver marker enzyme (ALT and AST) activities, andbrain tissue MDA, NO, DNA fragmentation, 8-OHdG and caspase-3 in both tramadol therapeutic and overdosegroups. However, brain tissue antioxidant enzyme (SOD and CAT) activities were markedly decreased.Tramadol withdrawal for two weeks produced significant improvement of all previous parameters towardsits normal ranges. These results proved that, tramadol administration for 9 weeks induced liver dysfunctionand brain injury in rats, as well as a degenerating endogenous antioxidant defense system mechanism. Thesefindings are of importance to be considered in patients who use tramadol as a pain killer, especially in thelong term conditions.}, keywords = {Neurotoxicity,Oxidative Stress,tramadol}, url = {https://bvmj.journals.ekb.eg/article_30017.html}, eprint = {https://bvmj.journals.ekb.eg/article_30017_f8e80cdb10acd4a798876d21ffdf686d.pdf} } @article { author = {Abd El-Tawab, Ashraf and Hassan, Wafaa and El-Saidi, Fawzi and Ayoup, Dalia}, title = {Department of Biochemistry, Faculty of Vet. Med., Benha University, Egypt}, journal = {Benha Veterinary Medical Journal}, volume = {33}, number = {2}, pages = {160-173}, year = {2017}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2017.30019}, abstract = {A total of 100 sample were obtained from different slaughter houses located in cairo, Living chickens(20 cloacal swabs ), Viscera (20 cecum and 20 internal organs ), 20 swabs from Chilling water and 20 swabs from final product, The samples were examined bacteriologically and serologically for Salmonella. where was 4% from total samples . The serotyping was two S.kentuky , one S.Tamilandu and one S.Anatum. The four isolated salmonella serovars were tested for antibiotic sensitivity and was found one isolate of Salmonella Kentuky was sensitive to all antibiotics while the other one was resistant to all antibiotics except (neomycin) was intermediate , S.Tamilandu was sensitive to all antibiotics except ( neomycin and colistin sulphate ) was resistant , S. Anatum was sensitive to neomycin, norfloxacin and ciprofloxacin , while resistant to nalidixic acid , cefatoxine ,ceftriaxon , gentamycin , amoxicillin and colistin sulphate and intermediate to ampicillin.Real time PCR was used for detection of salmonella, the positive Salmonella reference strain was ATCC 14028 and negative control was E.coli ATCC 25922. The number of positive Salmonella samples which was detected by RT-PCR was six samples while was four samples was No. of positive samples detected by stander method.}, keywords = {Salmonella,Real-Time PCR,chickens,slaughter houses}, url = {https://bvmj.journals.ekb.eg/article_30019.html}, eprint = {https://bvmj.journals.ekb.eg/article_30019_fd511bc4950d6798d95c060b3a00932c.pdf} } @article { author = {F., El-Bagoury and S., El-Habbaa and M., Ibrahim and Yousef, Noha,}, title = {Evaluating humoral immune response of sheep vaccinated with inactivated RVF virus vaccine using oil nanoparticles adjuvant in comparison with aluminum hydroxide gel}, journal = {Benha Veterinary Medical Journal}, volume = {33}, number = {2}, pages = {174-182}, year = {2017}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2017.30025}, abstract = {In the present study an inactivated tissue culture adapted RVF virus vaccine was prepared usingMontanide oil IMS 1313 nanoparticles as adjuvant then evaluated in sheep in comparison to the local RVFvirus vaccine with aluminum hydroxide gel adjuvant using serum neutralization test (SNT) and EnzymeLinked Immuno-Sorbent Assay (ELISA). The prepared vaccine was sterile and safe inducing no systemic orlocal clinical signs in sheep. Results indicated that the prepared nanoparticles oil based vaccine induced aprotective neutralizing serum antibody titer from the 2nd week post vaccination (WPV), reached the highestlevel at the 3rd month post vaccination (MPV) and persisted in protective level until the for 9th MPV, whilealuminum hydroxide gel based vaccine appeared in protective level at 2nd WPV, reached the highest level atthe 2nd MPV and persisted in protective level till the 7th MPV. These results were confirmed by using ELISA.These results demonstrated the potentiality of the prepared nanoparticles oil based vaccine compared to thelocally produced aluminum hydroxide gel based vaccine reflected by greater immunogenicity and longerduration of immunity.}, keywords = {RVF virus vaccines,adjuvants,sheep,SNT,ELISA}, url = {https://bvmj.journals.ekb.eg/article_30025.html}, eprint = {https://bvmj.journals.ekb.eg/article_30025_f963186bba7decf47dc2e0f0d94aa4cf.pdf} } @article { author = {F., El-Bagoury and S., El-Habbaa and M., Ibrahim and Yousef, Noha}, title = {Assessment of sheep immune response to an improved inactivated RVF virus vaccine with Montanide oil IMS 1313 nanoparticles adjuvant}, journal = {Benha Veterinary Medical Journal}, volume = {33}, number = {2}, pages = {183-192}, year = {2017}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2017.30267}, abstract = {Rift Valley Fever (RVF) virus belonged to genus Phlebovirus, family Bunyaviridae cause an acute,febrile disease in ruminants. In Egypt, a locally prepared inactivated RVF virus vaccine withaluminum hydroxide gel adjuvant has long been used for immunization of susceptible animalsagainst the disease. The choice of an adjuvant which gives high and long standing immune responseassume a critical part in immunization. The present study aimed to develop a new RVF virusvaccine with Montanide oil IMS 1313 nanoparticles as adjuvant and evaluate its impact on cellularand humeral immune response in sheep. The prepared vaccine was sterile and safe. SNT resultsdemonstrated that the prepared vaccine induced protective neutralizing serum antibody titer fromthe 2nd week post vaccination (PV), reached the highest level at the 3rd month PV and persisted inprotective level until the 9th month PV. These results were confirmed using ELISA. Evaluation ofRVF virus-specific cell-mediated immunity in vaccinated sheep using XTT assay showedsignificant lymphocyte proliferation expressed by optical density in vaccinated sheep group thatincreased to reach a maximum 10th day PV. Results indicated that the Montanide IMS 1313 VGNPR oil based vaccine induced high immunological enhancement without toxicity and with longduration of immunity that extended for 9 months.}, keywords = {RVF virus vaccine,Montanide IMS 1313 VG NPR oil,sheep}, url = {https://bvmj.journals.ekb.eg/article_30267.html}, eprint = {https://bvmj.journals.ekb.eg/article_30267_f3d7a0c729ee752cd2156e634551c698.pdf} } @article { author = {Abd El-Fadil, Maha and El Hawary, Rasha and Warda, Fatma}, title = {Keeping quality of inactivated respiratory virus vaccines (Pneumo-5)}, journal = {Benha Veterinary Medical Journal}, volume = {33}, number = {2}, pages = {193-199}, year = {2017}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2017.30270}, abstract = {In the keeping quality and the Efficacy of freshly locally prepared Bovine Respiratory virus vaccines(Pneumo-5) adjuvanted with either Aluminum Hydroxyl gel or montanoid oil , the serum neutralization testwas used for humoral immunity evaluation using( potency sample value) as a parameter by studying theeffect of different storage temperature on change on this value and its validity. it was found that the Geladjuvanted pneumo-5 vaccine was valid for( 15 month, 2 week and 1week) in storage temperature 4-8C(refrigerator) , 18-25C( room temperature) and 37C (incubator) respectively but we found that the Oiladjuvanted pneumo-5 vaccine was valid for ( 24 month, 3 week and 2 week) in storage temperature 4-8C(refrigerator) , 18-25C( room temperature) and 37C( incubator) respectively after that the vaccine becomesinvalid .}, keywords = {}, url = {https://bvmj.journals.ekb.eg/article_30270.html}, eprint = {https://bvmj.journals.ekb.eg/article_30270_1a5eaee2910c3851a5be06b2650c014a.pdf} } @article { author = {Ahmed, Nagwa and Al–Akabway, Lubna and Ramadan, Mohamed and Abd El-Gawad, Samah and Moustafa, Mahmoud}, title = {Serological and PCR-sequencing assays for diagnosis of Toxoplasma gondii and Neospora caninum infecting camels in Egypt}, journal = {Benha Veterinary Medical Journal}, volume = {33}, number = {2}, pages = {200-210}, year = {2017}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2017.30466}, abstract = {This study aimed to determine and evaluate the prevalence of the hidden coccidian tissue parasites (Toxoplasma gondii and Neospora caninum) in camels. For this purpose, 120 blood samples from two breed of camels (60Baldy and 60 Sudani) were collected through a period extend from September 2014 to August 2015. Samples were examined serologically for detection of anti-Toxoplasma gondii antibodies and by using PCR application for detection of Neospora caninum for the first time in Egypt. Results of indirect haemagglutination inhibition (IHAT) revealed that 6 out of 120 (5%) serum samples were seropositive and the infection rate was the highest in both autumn and winter. No significance effect of age (p>0.05) on the prevalence of infection among the examined camels, while the results of indirect Enzyme-Linked Immunosorbent assay (IELISA) showed that 63 out 120 serum sample examined by IHAT were positive (52.5%). Using PCR application of 50 blood samples revealed that 12 (24%) were positive for NC-5 region of Neospora caninum genome. Four positive purified PCR products were chosen randomly and for gene sequencing. The obtained sequences were registered in Gen Bank under accession numbers from MF980926.1 to MF980929.1. The analysis of DNA sequencing revealed that the obtained sequences were related to Neospora caninum with identity ranged from 98 and 99%. we concluded that both ELISA and PCR-sequencing were sensitive methods in the diagnosis of the tissue parasites.}, keywords = {toxoplasma gondii,Neospora caninum,IHAT,ELISA,PCR-sequencing}, url = {https://bvmj.journals.ekb.eg/article_30466.html}, eprint = {https://bvmj.journals.ekb.eg/article_30466_827b7b9349c00bf8c917ffae0fbca23d.pdf} } @article { author = {Hussein, Samy and Abdel Aal, Samir and Abd El-Aziem, Mohammed and EL Said, Hisham}, title = {Ameliorating role of resveratrol on biochemical changes in experimentallyinduced liver fibrosis in rats}, journal = {Benha Veterinary Medical Journal}, volume = {33}, number = {2}, pages = {211-223}, year = {2017}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2017.30469}, abstract = {Liver fibrosis is the final pathway stage of most chronic liver diseases, and is the main reason forincreased mortality in affected patients. Hence, this study was undertaken to evaluate thehepatoprotective effect and the efficacies of resveratrol against thioacetamide (TAA) induced liverfibrosis in rats. The experimental model of liver fibrosis in rats was induced by intraperitoneal (i.p)injection of TAA at a dose (200mg/Kg b.wt / thrice / week) for 6 weeks. A total of fifty five malerats were used in this study. All rats were divided into four groups. Group I: (Control normalgroup) rats received no drugs. Group II: (TAA- group). Group III: (TAA + resveratrol protectedgroup) rats injected with TAA and administered resveratrol (0.5 mg/Kg body weight / day,intraperitoneally) from the 7th week to 12th week. Group IV: (TAA + resveratrol treated group) ratsfirstly injected with TAA from the 1st week to 6th week and treated daily with resveratrol (0.5mg/Kg body weight, intraperitoneally) from the 7th week to 12th week. Blood samples and livertissue were taken and processed directly for some biochemical parameters determination. Theobtained results revealed that, a significant increase in serum ALT, AST and ALP activities, IL-6,TGF-β1 and liver L-MDA concentrations were observed in TAA injected rats. However,administration of resveratrol to TAA induced liver fibrosis in rats exhibited a significant decreasedin all mentioned parameters and attenuated the increased L-MDA concentration in liver tissues. Onthe other hand, a significant decrease in liver antioxidant enzymes (SOD and CAT) activities wereobserved in TAA injected rats when compared with control normal group. Meanwhile, resveratroladministration resulted in a significant increase in liver SOD and CAT activities. It could beconcluded that, inhibition of lipid peroxidation, inflammation and oxidative stress and enhancedantioxidant enzymatic status in rats liver by resveratrol suggest the potential efficiency ofresveratrol as a natural hepatoprotective and anti-inflammatory agent in treatment of liver fibrosis.}, keywords = {Thioacetamide (TAA),resveratrol (RSV),Oxidative Stress,inflammatory markers,Liver fibrosis}, url = {https://bvmj.journals.ekb.eg/article_30469.html}, eprint = {https://bvmj.journals.ekb.eg/article_30469_064dae965663011f56fb3c6668bbc708.pdf} } @article { author = {Ghanem, Prof Mohamed and A., Fares and M., Abdel-Raof and E., El-Attar}, title = {Clinico-biochemical, oxidative markers and trace elements changes in cows with ketosis}, journal = {Benha Veterinary Medical Journal}, volume = {33}, number = {2}, pages = {224-236}, year = {2017}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2017.30475}, abstract = {Ketosis was diagnosed in 10 lactating cows in private farm in Qaluabia government based on the blood level of Non-esterified fatty acid (NEFA) and Beta hydroxy butyric acid (BHBA). Another 10 healthy cows with normal NEFA and BHBA were used as control. Clinically, the affected cows had partial or complete anorexia with reduction of rumen movement. Biochemically, there was a significant (P< 0.05) increase in AST, ALT, creatinine in addition to NEFA and BHBA in ketotic group compared to control. On the other hand, there was significant decrease (P < 0.05) in triacyl glyceride, albumin, copper and zinc. The oxidative stress markers showed significant increase (P <0.05) in MDA concentration and significant decrease in SOD activity. It was concluded that ketosis is related to clinical, biochemical, oxidative stress and reduced serum Cu and Zn levels. It is recommended to add trace elements, specially serum Cu and Zn in the periparturient period.}, keywords = {Anti-oxidant,biochemical,copper,ketosis,zinc}, url = {https://bvmj.journals.ekb.eg/article_30475.html}, eprint = {https://bvmj.journals.ekb.eg/article_30475_c92f63343a9be4e8c09dde60e4375bc9.pdf} } @article { author = {M., Fararh and S., Farid and S., Abd EL-Hamied and B., El-Sharkawy}, title = {Clinicopathological changes in calves with respiratory diseases after treatment with essential volatile oil and other drugs}, journal = {Benha Veterinary Medical Journal}, volume = {33}, number = {2}, pages = {237-247}, year = {2017}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2017.30477}, abstract = {This study was performed on fifteen newly born calves, 3-5-month-old with clinical signs of bronchopneumonia in addition to five clinically healthy calves which considered as control group. After bacterial isolation and identification of the causative microorganisms, fifteen calves from which was found infected with Pasteurella spp. and Haemophilus spp. The diseased calves were divided randomly into three equal groups which respectively, treated with: Gp.1: were treated dose of draxxin (2.5 mg/kg body weight) by s/c injection in the neck; Gp.2: were received Draxxin and Meloxicam (single I/M injection of 0.5 mg/kg b.wt.), while Gp.3: were received draxxin (single s/c injection of 20 mg/kg b.wt.) +Symopro on drinking water (150-300 ml/1000 L for 5 days). Blood and serum samples were collected from all calves just before treatment, 3 and 10 days post treatment. pH, PO2 and SO2 results recorded significant increase in all treated groups. Meanwhile, PCO2, tCO2 and HCO3 results showed significant decrease in all treated groups compared with diseased group before treatment. Haptoglobin and fibrinogen results showed significant decrease in all treated groups compared with diseased group before treatment. GSH-PX results revealed significant increase. Meanwhile, MDA results showed significant decrease in all treated groups which comparatively with those before treatment. There was significant increase in cellular immunity (phagocytic index and phagocytic%) in treated group with draxxin plus sympro when compared with diseased group before treatment.}, keywords = {Non-steroidal anti-inflammatory drug,Bovine respiratory diseases,Sympro,Malondialdhyde,glutathione peroxidase}, url = {https://bvmj.journals.ekb.eg/article_30477.html}, eprint = {https://bvmj.journals.ekb.eg/article_30477_b7f6a360f537746adbdc290371d477de.pdf} } @article { author = {El-Bagoury, Gabr and El-Habbaa, Ayman and El-Sayed, Effat and Abd El-Aziz, Manal}, title = {Improving performance of Pneumo-4 vaccine using montanide oil}, journal = {Benha Veterinary Medical Journal}, volume = {33}, number = {2}, pages = {249-258}, year = {2017}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2017.30479}, abstract = {Selecting of the adjuvant is one of the keys for the success of the vaccine application. Oil adjuvants, water-in-oil-in-water (W/O/W), have proved their efficacy in bovine vaccine as they induce rapid and long term immune response with various antigens. The present study was aimed towards improving and upgrading the locally prepared inactivated combined vaccine (Pneumo-4) containing Infectious Bovine Rhinotracheitis (IBR), Bovine Viral Diarrhoea (BVD), Parainfleunza type 3 (PI-3) and Bovine Respiratory syncytial (BRS) viruses using Montanide oil ISA206 as adjuvant and compare its efficacy with the commercial gel adjuvanted one. Both the commercial and prepared oil Pneumo-4 vaccines were administered twice to 6-9 months old seronegative calves through intramuscular (I/M) route with dose 5 ml, 21 days apart. Humoral immune response was followed up in the sera of vaccinated calves using serum neutralization test (SNT). The result revealed that both adjuvanted vaccines gave a significant effect on all vaccinated animals but the oil adjuvanted vaccine was particularly better and effective than gel adjuvanted where oil adjuvanted vaccine induced early intense immune response and showed prolongation of antibody secretion in comparison to the Aluminium Hydroxide gel.}, keywords = {pneumo-4 vaccine,ISA 206,SNT}, url = {https://bvmj.journals.ekb.eg/article_30479.html}, eprint = {https://bvmj.journals.ekb.eg/article_30479_cc6935ec76559f4f4fed4046877ae512.pdf} } @article { author = {Hussein, Samy and Ahmed, Tahia and Ali, Aliaa}, title = {Spirulina platensis protects against L-methionine induced hyperhomocysteinemia by abrogation of oxidative stress markers, hyperhomocysteine and inflammation in rats}, journal = {Benha Veterinary Medical Journal}, volume = {33}, number = {2}, pages = {257-270}, year = {2017}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2017.30483}, abstract = {Spirulina platensis have therapeutic functions such as antioxidant, anti-bacterial, antiviral, anticancer, anti-inflammatory, anti-allergic and anti-diabetic. The purpose of this study was to evaluate the protective effect of spirulina platensis on L-methionine induced hyperhomocysteinemia (HHcy) in rats. Thirty male albino rats were divided into three equal groups. Group I (control normal): rats administered distilled water. Group II (hyperhomocysteinemic group (HHcy): rats received L-methionine (1.7 gm/kg body weight/day) orally for continuous 8 weeks. Group III (HHcy + spirulina platensis): rats received spirulina (1.5 gm/kg body weight/day) orally for 4 weeks after induction of hyperhomocysteinemia. The obtained results showed significant increase in serum homocysteine, lipids profile (total cholesterol and triacylglycerols), liver marker enzymes (ALT, AST and ALP), liver L-MDA and pro-inflammatory cytokines (TNF-α and IL-8) gene expression level in hyperhomocysteinemic (HHcy) rats. However, liver SOD activity and GSH concentration were markedly decreased. Spirulina treatment to L-methionine induced HHcy in rats caused significant improvement of all previous parameters towards its normal ranges. These results suggested that, spirulina treatment exerts a protective effect on HHcy by reduction of oxidative stress marker, inflammation and hyperhomocysteinemia in rats through free radical scavenging and anti-inflammatory activities as well as regenerating endogenous antioxidant defense system mechanisms.}, keywords = {L-methionine,Hyperhomocysteinemia,Spirulina,Oxidative Stress}, url = {https://bvmj.journals.ekb.eg/article_30483.html}, eprint = {https://bvmj.journals.ekb.eg/article_30483_418d43e4f2f7598ca3604713d9d298f2.pdf} } @article { author = {Hussein, Samy and Ali, Aliaa and Ahmed, Tahia}, title = {The Potential protective effect of crocin against hyperhomocysteinemia induced oxidative stress in rats}, journal = {Benha Veterinary Medical Journal}, volume = {33}, number = {2}, pages = {271-282}, year = {2017}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2017.30485}, abstract = {Crocin is the major yellow pigment of saffron and gardenia yellow, which are extracts of Crocus sativus stigmas and Gardenia jasminoides fruits, crocin can prevent chronic stress induced oxidative stress damage in the brain, liver and kidneys, the purpose of this study was to evaluate the protective and anti-inflammatory effect of crocin against L-methionine induced hyperhomocysteinemia and oxidative stress in rats. Thirty male albino rats were divided into three equal groups. Group I (normal group): rats administered distilled water. Group II (L-Methionine induced hyperhomocysteinemia (HHcy): rats received L-methionine (1.7 g/kg b.w) orally daily for continuous 8 weeks. Group III (HHcy + crocin treated group) rats received crocin for 4 weeks after induction of hyperhomocysteinemia inrta peritoneal once per day at a dose of (50 mg/kg body weight/day, I.P). The obtained results showed significant increase in serum homocysteine, lipid profile (cholesterol, triglyceride), liver enzymes (ALT, AST and ALP) activities, liver tissue L-MDA levels and inflammatory markers (TNF-α and IL-8) in hyperhomocysteinemic (HHcy) rats. However, activities of liver tissue antioxidant enzymes SOD and GSH concentration were markedly decreased. Administration of crocin to HHcy rats caused significant improvement of all previous parameters towards its normal ranges. These results suggested that, crocin treatment may have a protective effect against hyperhomocysteinemia induced oxidative stress in rats through free radical scavenging and anti-inflammatory activity as well as regenerating endogenous antioxidant defense system mechanisms.}, keywords = {Hyperhomocysteinemia,Crocin,Oxidative Stress}, url = {https://bvmj.journals.ekb.eg/article_30485.html}, eprint = {https://bvmj.journals.ekb.eg/article_30485_e063b94e439bfaf1588ab9f9c2a88cb9.pdf} } @article { author = {A., Shaltout and M., Zakaria and E., Nabil}, title = {Detection and typing of Clostridium perfringens in some retail chicken meat products}, journal = {Benha Veterinary Medical Journal}, volume = {33}, number = {2}, pages = {283-291}, year = {2017}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2017.30487}, abstract = {Clostridium perfringens is considered as one of major food poisoning bacteria; which may refer to different lethal toxins production including C. perfringens enterotoxin. C. perfringens toxins have been contributed in many diseases in human being especially C. perfringens type A enterotoxin food poisoning. A total of 125 random raw and half cooked chicken meat samples represented by (breast, thigh, nuggets, panée and frankfurter “25 of each”) were collected from various retail stores and supermarkets in Qualyubia governorate to investigate the presence of C. perfringens bacteriologically and detect the cpa, etx, and cpe toxin genes by multiplex PCR. Results demonstrated that 6 out of 25 raw breast samples (24%), 8 out of 25 raw thigh samples (32%), 5 out of 25 nuggets samples (20%), 4 out of 25 panée samples (16%), and 4 out of 25 frankfurter samples (16%) were found to be contaminated with C. perfringens. Twenty-seven positive isolates obtained from these samples were identified as C. perfringens based on the microscopic examination and biochemical tests. It was detected that 8 (29.6%) out of 27 C. perfringens isolates carried only alpha toxin gene (type A), and only one isolate (3.7%) of them expressed both alpha and epsilon toxin genes (type D); while cpe gene never had been detected in any examined isolate, according to the multiplex PCR results.}, keywords = {Chicken Meat Products,C. perfringens,Multiplex PCR}, url = {https://bvmj.journals.ekb.eg/article_30487.html}, eprint = {https://bvmj.journals.ekb.eg/article_30487_f3ea5d233c5abf054e24dc0730dd4606.pdf} } @article { author = {A., Shaltout and M., Zakaria and E., Nabil}, title = {Incidence of some anaerobic bacteria isolated from chicken meat products with special reference to Clostridium perfringens}, journal = {Benha Veterinary Medical Journal}, volume = {33}, number = {2}, pages = {292-304}, year = {2017}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2017.30489}, abstract = {Anaerobic spore formers, especially Clostridium perfringens, represent one of the most prevalent bacterial food poisoning outbreaks which mostly related to consumption of contaminated meat and meat products, therefore, a total of 125 random raw and half cooked chicken meat samples represented by (breast, thigh, nuggets, panée and frankfurter “25 of each”) were collected from various retail stores and supermarkets in Qualyubia governorate. Results illustrated that, raw thigh samples were the most contaminated with anaerobic bacterial counts in incidence of 84%. The identified strains were C. perfringens, C. sporogenes, C. bifermenants, C. butyricum and C. sordelli in 21.6, 16, 8, 3.2 and 3.2%, respectively. Regarding to the incidence of vegetative and spore of C. perfringens were 24, 32, 20, 16, 16% and 16, 20, 16, 8, 8% in examined raw breast, raw thigh, nuggets, panée and frankfurter, respectively. 33.3% of isolates were lecithinase positive strains and typed as C. perfringens type A (6.4%), type D (0.8%); in absence of neither type B nor D. Experimental heat resistant C. perfringens spores were six heat resistant strains; where all isolates were of type A. The high incidence of these food poisoning microorganisms in chicken meat may indicate defects in sanitary conditions and handling in processing plant.}, keywords = {Chicken meat,Clostridium perfringens,heat resistant spores,Nagler’s reaction,other anaerobes}, url = {https://bvmj.journals.ekb.eg/article_30489.html}, eprint = {https://bvmj.journals.ekb.eg/article_30489_c9bf0d92c5ebd618a538ced9f0e40aae.pdf} } @article { author = {A., Edris and Hassanien, Fatin and Shaltout, Fahim and ELbaba, Azza and Adel, Nairoz}, title = {Microbiological evaluation of some heat treated fish products in Egyptian markets}, journal = {Benha Veterinary Medical Journal}, volume = {33}, number = {2}, pages = {305-316}, year = {2017}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2017.30491}, abstract = {This study was conducted to confirm the bacterial conditions of fish products with E.O.S, and theirhazards on public health. A total of 60 samples of fish products represented by smoked: herring andsmoked salmon– semi cooked: fish finger and breaded shrimp (15 of each) were collected fromdifferent retail markets for bacteriological examination. The average of APC, Coliform, Escherichiacoli, Mould & yeast and Staphylococcus aureus counts (log10 cfu/g) were 4.17± 0.12, 2.92±0.16,2.19±0.23, 3.96±0.14 and 1.72±0.21 for herring, respectively, 3.16± 0.19, 2.69±0.13, 1.22±0.16,2.22±0.18 and 1.06±0.06 in smoked salmon, 2.78±0.12, 2.02±0.22, 1.59±0.22, 2.14±0.15 and1.24±0.24 in fish finger, respectively, and 2.60±0.13, 2.33±0.14, 1.46±0.23, 1.96± 0.20, 0± 0 inbreaded shrimp, respectively. The incidence of some food poisoning bacteria (Salmonella, Listeriamonocytogenes and Vibrio parahaemolyticus also investigated and no one of them was isolated inthe examined samples.}, keywords = {Salmon,Herring,Fish finger,Breaded shrimp}, url = {https://bvmj.journals.ekb.eg/article_30491.html}, eprint = {https://bvmj.journals.ekb.eg/article_30491_1dcfd100bddd2bb2ff016e6beabe70f0.pdf} } @article { author = {A., Edris and Hassanien, Fatin and Shaltout, Fahim and ELbaba, Azza and Adel, Nairoz}, title = {Microbiological evaluation of some frozen and salted fish products in Egyptian markets}, journal = {Benha Veterinary Medical Journal}, volume = {33}, number = {2}, pages = {317-328}, year = {2017}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2017.30493}, abstract = {Sixty random samples of fish and fish products (frozen fish: Tilapia nilotica and imported basa fillet– salted fish: Mugil cephalus and sardine) (15 of each) were collected from different supermarketsin Egypt. All collected samples were microbiologically examined for determination of AerobicPlate Count (APC), coliforms, Escherichia coli, Mould & yeast and Staphylococcal aureuscounts (log10 cfu/g). Isolation and identification of salmonella, liesteria monocytogenes and Vibrioparahaemoliticus. The bacteriological examination revealed that the mean values of APC in theexamined fish samples were 4.80± 0.16, 4.63±0.18, 2.35±0.18 and 2.70±0.13 (log10 cfu/g) forfrozen Tilapia nilotica, Basa salted Mugil cephalus and Sardine respectively, 1.48± 0.22, 2.19±0.19,2.10±0.16 and 2.52±0.11 average coliform counts in examined samples respectively, and E.colicount with an average of 2.52±0.14, 2.01±0.17, 1.31±0.11and 1.64±0.14. Mould & yeast averagecounts were 0.93±0.18, 1.22±0.16, 1.67±0.021 and 1.69±0.18 in examined fish samples,respectively, and the Staphylococcal counts were 1.30±0.17, 1.58±0.26, 2.12±0.14, 1.46±0.23 and2.17±0.17 in examined fish samples, respectively. The incidence of food poisoning organisms(Salmonella & Listeria monocytogenes and Vibrio parahaemolyticus also was investigated and oneof both Listeria monocytogenes and Vibrio parahaemolyticus were isolated in frozen Basa filletsamples with a percentage of 33% and 33% from the examined samples.}, keywords = {frozen fish: Tilapia nilotica and Basa,salted fish: Mugil cephalus and Sardine}, url = {https://bvmj.journals.ekb.eg/article_30493.html}, eprint = {https://bvmj.journals.ekb.eg/article_30493_ff6f047b2f454690b9a10465411c15c2.pdf} } @article { author = {Abd El-Fatah, Salwa and Bakry, Hatem and Abo Salem, Mohamed and Hassan, Atef}, title = {Comparative Study between the Use of Bulk and Nanoparticles of Zinc Oxide in Amelioration the Toxic Effects of Aflatoxins in rats}, journal = {Benha Veterinary Medical Journal}, volume = {33}, number = {2}, pages = {329-342}, year = {2017}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2017.30496}, abstract = {The ability of zinc oxide nanoparticles (ZnO-NPs) to ameliorate the toxic effect of aflatoxin B1(AFB1) in rats in comparison with traditional bulk zinc oxide was evaluated in this study. Twodoses of bulk zinc oxide and ZnO-Nps (25 and 50 μg/kg body weight rat of each) were given orallyto rats experimentally treated with 50μg/kg b.wt rat AFB1 produced by aflatoxigenic Aspergillusflavus strains. The experimental study extended for further 30 days. The obtainedresults revealedthat the low doses of zinc oxide nanoparticles were more effective than low or high doses of bulkZnO in amelioration the toxic effects of aflatoxicosis which reflected as enhancement in antioxidantactivity and histopathological picture of aflatoxicated rats.}, keywords = {ZnO-NPs- Aspergillus flavus- aflatoxin B1- aflatoxicosis,amelioration}, url = {https://bvmj.journals.ekb.eg/article_30496.html}, eprint = {https://bvmj.journals.ekb.eg/article_30496_1ffa32f057d6189325465bd885741fbb.pdf} } @article { author = {Hassanin, Faten and A., Hassan and Elsheikh, Nabila and Amin, Tereza}, title = {Rancidity as Lipolytic Index in Poultry Meat Cuts}, journal = {Benha Veterinary Medical Journal}, volume = {33}, number = {2}, pages = {343-350}, year = {2017}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2017.30498}, abstract = {Ninety random samples of fresh poultry meat cuts represented by chicken thigh & breast, duck thigh & breast and turkey thigh & breast (15 of each) were collected from different slaughtered shops Tanta city, Gharbia government. The collected samples were analyzed for determination of thiobarbiturc acid (TBA), perioxide value (P.V.) free fatty acids (FFAs) and fractionation of fatty acids to determine their lipolytic indices for their keeping quality as well as their composition of fatty acids. The obtained results showed that mean values for duck thigh, chicken thigh and turkey thigh samples were 0.52+0.03, 0.33+0.02 and 0.15+0.01 (mg/kg) for TBA & 0.85+0.09, 0.57+0.07 and 0.33+0.04 (mgO2/kg) for PV, respectively. Plus, 0.69+0.07, 0.45+0.06 and 0.28+0.02 (mg %) for FFAs, respectively. Concerning breast of both duck, chicken and turkey samples, the mean values were 0.40+0.02, 0.19+0.01 and 0.12+0.01 (mg/kg) for TBA, where, 0.64+0.07, 0.41+0.05 and 0.27+0.02 (mgO2/kg) for PV. As well as, 0.52+0.05, 0.36+0.03 and 0.20+0.03 (mg %) for FFAs respectively. Finally, samples subjected to fractionation of fatty acids the ratio between total unsaturated fatty acids and total saturated fatty acids were 0.96, 1.23 and 1.66 for thigh of duck, chicken and turkey, respectively. While breast of duck, chicken and turkey ratios were 1.15, 1.45 and 2.23 respectively. Turkey breast meat had the highest keeping quality and nutritive value compared with the other samples.}, keywords = {tba,PV,FFA,Poultry}, url = {https://bvmj.journals.ekb.eg/article_30498.html}, eprint = {https://bvmj.journals.ekb.eg/article_30498_59ae809cfc162008081e61137e7cfad9.pdf} } @article { author = {Shaltout, Fahim and G., Thabet and Koura, Hanan}, title = {Impact of some essential oils on the quality aspect and shelf life of meat}, journal = {Benha Veterinary Medical Journal}, volume = {33}, number = {2}, pages = {351-364}, year = {2017}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2017.30503}, abstract = {Plant essential oils (EOs) serve as a "safe" alternative to chemical or synthetic antimicrobials and antioxidants to struggle with the food borne pathogens or spoilage organisms, inhibiting lipid oxidation and thus extend shelf life of ground beef application .In our study, we investigated the effectiveness of thyme oil (thymus vulgaris) at concentrations (1% , 1.5% and 2%) and cinnamon oil (cinnamomum zeylanicum) at concentrations (0.5% , 1% and 1.5%) as natural preservatives as well as their ability to increase the shelf life of minced meat , and also their effects on chemical , microbial analysis and sensory properties of minced meat when been stored at 2°C for 12 days storage period. Obtained results showed that samples treated revealed decreasing of values from chemical, microbial and improving sensory properties than untreated samples (control) ones. Also, thyme oil at concentration 2% and cinnamon oil at concentration 1.5% showed best values than others.}, keywords = {Essential oils,thyme oil and cinnamon oil,Minced meat}, url = {https://bvmj.journals.ekb.eg/article_30503.html}, eprint = {https://bvmj.journals.ekb.eg/article_30503_a37aff82e4118681eead68d188c7ba9e.pdf} } @article { author = {Abouzaid, Omayma and El Shawi, Omama and Mansour, Nahla and Hanafy, Aml}, title = {Synthesis and Characterization Of Dextran Coated Magnetite Nanoparticles Loaded With Curcumin And In Vitro Cytotoxicity Study In MCF-7 Cancer Cells}, journal = {Benha Veterinary Medical Journal}, volume = {33}, number = {2}, pages = {365-374}, year = {2017}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2017.30506}, abstract = {Medicinal plants are considered to be bioactives valuable sources of anti-cancer. But these compounds are hydrophobic in nature and thus show decreased bioavailability. To overcome this issue, biocompatible drug delivery agents could be conjugating with these bioactives to improve their therapeutic efficacy. In the present study Curcumin loaded dextran coated Fe3O4NPs was synthesized by chemical precipitation method and coated with Dextran (DEX) using diffusion method. The structural, morphological and the magnetic properties of the prepared materials were studied by using Fourier transform infrared (FT-IR) spectro- scopy, transmission electron microscopy (TEM), X-ray diffraction (XRD), dynamic light scattering (DLS), zeta potential and Vibrating Sample Magnetometer (VSM). The MTT (3-(4,5-dimethylthiahiazol-2-yl)-2,5-diphenyl tetrazolium) assay of CUR loaded dextran coated magnetite nanoparticles (CUR/DEX/Fe3O4)NPs exhibited notable toxicity against MCF7 cells in a dose and time dependant manner. The IC50 values were 177μg/ml and 109μg/ml after 24h and 48h incubation respectively}, keywords = {Curcumin,Dextran,Fe3O4,nanoformulation/ anticancer}, url = {https://bvmj.journals.ekb.eg/article_30506.html}, eprint = {https://bvmj.journals.ekb.eg/article_30506_ebd3e98b63b2d4d8b600e05402e88a4c.pdf} } @article { author = {Abouzaid, Omayma and Badwi, Abdel Fatah and Gabr, Hanaa}, title = {Biochemical effect of New Novel Composite on 7,12 dimethylbenz (a) anthracene Induced Mammary Carcinogenesis in Rats}, journal = {Benha Veterinary Medical Journal}, volume = {33}, number = {2}, pages = {375-383}, year = {2017}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2017.30508}, abstract = {Breast cancer is the second leading cause of cancer death in women, There are several ways to treat breast cancer .The effect of Lysine-Cetrimonium gallium Complex with zinc oxide nanopatricle and sodium ascorbate (NNC) on DMBA induced mammary carcinogenesis was investigated in female rats. Oral dose of DMBA induced a significant increase in ALT, AST, MDA and serum NO levels. On other hand marked depletion in CAT, GSH. On other hand oral administration of NNC was able to mitigate mammary carcinogenic damage induced by DMBA as evidence to pronounce curative effect against lipid peroxidation, serum NO level and deviated enzymatic variables and maintained glutathione status, antioxidant enzymes in addition histopathological changes directed toward control. We observed that the administration of (NNC) have potential to exert curative effects against mammary carcinogenesis}, keywords = {breast cancer,gallium chloride,Zinc Oxide Nanoparticles,CTAB}, url = {https://bvmj.journals.ekb.eg/article_30508.html}, eprint = {https://bvmj.journals.ekb.eg/article_30508_17717fa4562615f08da701efe4bd1690.pdf} } @article { author = {M., Fararh and S., Abd EL-Hamied and S., Farid and B., El-Sharkawy}, title = {Evaluation of levamisole efficacy as immune-stimulant on respiratory diseases treatment in calves}, journal = {Benha Veterinary Medical Journal}, volume = {33}, number = {2}, pages = {384-395}, year = {2017}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2017.30511}, abstract = {This study was performed on fifteen newly born calves, 3-5 month old with clinical signs ofbovine respiratory disease (BRD) in addition to five clinically healthy which considered as controlgroup. After bacterial isolation and identification of the causative microorganisms, fifteen calvesfrom which were found infected with E.coli, P. multocida, Klebsiella and S. aureus. The diseasedcalves were divided randomly into three equal groups: Gp.1: were treated dose of draxxin (2.5mg/kg body weight) by s/c injection in the neck; Gp.2: were received Draxxin and Meloxicam(single I/M injection of 0.5 mg/kgb.wt.), while Gp.3: were received draxxin (single s/c injection of20 mg/kg bwt.) +Levamisole (single I/M injection at a dose of 1mg/50kg bwt). Blood and serumsamples were collected from all calves just before treatment, 3 and 10 days post treatment. TLC,neutrophils and monocyte count revealed significant decrease in all treated groups in comparison withdiseased group before treatment. While, lymphocyte count recorded significant increase in all treatedgroups compared with diseased group and lastly, esinophil recorded significant decrease in treatedgroups with draxxin plus meloxicam and draxxin plus levamisole in comparison with diseased group.Total protein demonstrated significant increase in all treated in comparison with diseased group.Serum albumin, total globulin, α-globulin, β-globulin and γ-globulin showed significant decreasewhen compared with diseased calves before treatment. Haptoglobin and fibrinogen showedsignificant decrease in all treated groups in comparison with diseased group. There were significantincrease in immunoglobulins and cellular immunity (phagocytic index and phagocytic percentage) inall treated groups especially in group treated with draxxin plus levamisole when compared withdiseased group before treatment.}, keywords = {Bovine respiratory disease,Levamisole,Haptoglopin,Fibrinogen,Non-steroidal antiinflammatory drug}, url = {https://bvmj.journals.ekb.eg/article_30511.html}, eprint = {https://bvmj.journals.ekb.eg/article_30511_6838ad6b04c3105b09f2d9a2b8a7649a.pdf} } @article { author = {Abdel Mageid, Afaf and Abou Salem, Mohamed and Salaam, Nancy}, title = {The biochemical role of curcumin nano-suspension in the metabolism of experimentally induced diabetic rats}, journal = {Benha Veterinary Medical Journal}, volume = {33}, number = {2}, pages = {396-401}, year = {2017}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2017.30513}, abstract = {This study was designed to determine the biochemical effect of curcumin nano-suspension on alleviating metabolic disorders in experimentally induced diabetic rats. An experimental diabetic rat model was induced by 60mg/kg body weight of streptozoticin (STZ) as a single dose. Curcumin nano-suspension was administrated orally at a dose of 300mg/kg body weight daily for 56 days. Metabolic profile was examined biochemically. Administration of curcumin nano-suspension decreases CK-MB, LDH and AST serum values in STZ- diabetic rats treated with curcumin nano-suspension (203±5.04 and 604±12.85; 1910±7.97 and 4739±120.17, and 125± 12.13 and 174 ± 16.10) respectively, when compared to untreated diabetic rats, it decreases triacylglycerol in serum (124±2.01 and 144±11.4mg/dl) compared to the diabetic rats also, with not so much increase the body weight (242±4.94 and214±18.04gm). On the other hand, there were no changes in concentrations of glucose (346.50±11.04 and 329.50±42.86mg/dl); insulin (0.92±0.66 and1.10±0.18μl/ml) and the total cholesterol level (105±5.04and 112±17.18mg/dl) in serum compared to diabetic rats. In conclusion, oral ingestion of curcumin nano-suspension reverses side effects result from metabolic disorders in streptozotocin induced diabetic rats via its anti-inflammatory and hypolipidemic role.}, keywords = {diabetes,streptozotocin,curcumin nano-suspension}, url = {https://bvmj.journals.ekb.eg/article_30513.html}, eprint = {https://bvmj.journals.ekb.eg/article_30513_bbbf4e41985c344e9b2ea984e75f6d4e.pdf} } @article { author = {Hassanen, Fatin and Shaltout, Fahem and S., Amani and A., Maarouf, and A., Rasha}, title = {Studies on bacteriological profile of chicken meat cuts in Kaliobia governorate}, journal = {Benha Veterinary Medical Journal}, volume = {33}, number = {2}, pages = {402-409}, year = {2017}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2017.30514}, abstract = {The study was performed on 120 random samples of fresh chicken meat cuts of Drumstick, thigh and breast (40 of each) purchased from different supermarkets and retail chicken butchers in Kaliobia governorate to evaluate their bacterial contamination. The bacteriological examination revealed that the mean values of APC, Staphylococci aureus counts (cuf/g) in drumstick samples were 7.47×104 ±0.31 ×104; 2.31×103 ±0.11 ×103 & 0.26×103 ±0.07 ×103, 6.51×104 ±0.30×104; 2.21×103 ±0.11×103 in thigh& 0.22×103 ±0.07×103, and 6.13×104 ±0.32 ×104; 1.99×103 ±0.09 ×103& 0.19×103 ±0.06×103 in breast samples, respectively. Coagulase positive aureus strains were isolated from the examined fresh chicken meat cuts represented as 32.5% in drumstick 27.5% in thigh and 22.5% in breast samples. The isolated strains were highly resistant for methicillin followed by oxacillin; ampicillin; cefotaxime and amoxicillin. Meanwhile, they were highly sensitive to ciprofloxacin followed by enrofloxacin and gentamycin.}, keywords = {Chicken meat,Bacteriological Evaluation,S. aureus}, url = {https://bvmj.journals.ekb.eg/article_30514.html}, eprint = {https://bvmj.journals.ekb.eg/article_30514_4541d3bde196481b8b773b844ccf202d.pdf} } @article { author = {Hassanen, Fatin and Shaltout, Fahem and S., Amani and A., Maarouf and A., Rasha}, title = {Detection of virulence genes of enterotoxigenic Staphylococcus aureus isolated from chicken meat cuts by using PCR}, journal = {Benha Veterinary Medical Journal}, volume = {33}, number = {2}, pages = {410-417}, year = {2017}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2017.30515}, abstract = {The present study was performed on 120 random samples of fresh chicken meat cuts of Drumstick, thigh and breast (40 of each) which were purchased from different supermarkets and retail chicken butchers at Kaliobia Governorate, for detection of virulence genes responsible for Staphylococcus. aureus enterotoxins production by using PCR.A total of 33 examined samples were positive for S.aureus counts represented as 13(32.5%) from drumstick samples; 11(27.5%) from thigh samples and 9(22.5%) from breast samples. The results revealed that 8 strains out of 10 random examined strains by SET- RPLA test were enterotoxigenic and classified according to type of toxin into (3A;1C,2D and 2A&C).Moreover, PCR results cleared that; sea gene was detected in 4 studied strains; seb gene in one strain and sec; sed and see genes were detected in 3 strains out of 10 studied strains}, keywords = {Chicken meat,enterotoxigenic S.aureus,Virulence genes}, url = {https://bvmj.journals.ekb.eg/article_30515.html}, eprint = {https://bvmj.journals.ekb.eg/article_30515_a0560337726f87722b26e27b06b116f4.pdf} } @article { author = {Hussein, Samy and Ismail, Hany and Abdel Aal, Samir}, title = {Effect of tramadol drug on some biochemical and immunological parameters in albino male rats; evaluation of possible reversal following its withdrawal}, journal = {Benha Veterinary Medical Journal}, volume = {33}, number = {2}, pages = {418-429}, year = {2017}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2017.30589}, abstract = {The purpose of this study was to evaluate the effect of repeated tramadol drug administration on some biochemical and immunological parameters in rats. One hundred male albino rats were divided into three main groups. Group I (control): contains twenty rats administered saline solution. Group II (therapeutic dose group): forty rats received a daily oral dose of tramadol Hcl suspended in saline solution equal to 22.5 mg/kg/day for nine weeks. Ten rats of second group were left two weeks more without any additional treatment as a recovery period. Group III (over dose group): forty rats received oral dose of tramadol Hcl suspended in saline solution at doses of 30, 60 and 90 mg/kg/day on the first, second and third three weeks of the study, respectively for nine weeks. Ten rats of third group were left for two weeks more without any additional treatment as a recovery period. The obtained results showed significant increase in serum marker (Urea, creatinine, sialic acid, TNF-α and IL-1β) levels, and brain tissue (NF-KB and MPO) levels in both tramadol therapeutic and overdose groups towards its normal ranges. However, brain tissue antioxidant parameters (GPX and GSH) levels and liver tissue marker cyp2E1were markedly decreased. These results showed that, chronic tramadol administration induced kidney dysfunction, brain injury, oxidative and inflammatory stress in rats as well as degenerating endogenous antioxidant defense system mechanisms. These findings are of importance to be considered in the patients who use tramadol as a pain killer especially in the long term condition.}, keywords = {tramadol,Oxidative Stress,Neurotoxicity}, url = {https://bvmj.journals.ekb.eg/article_30589.html}, eprint = {https://bvmj.journals.ekb.eg/article_30589_d03b5fb0222e46b46f5abf193440c011.pdf} } @article { author = {Abdelnaser, Amal and Zyan, Kamel and El Basuni, Sawsan and El Nabarawy, Anwaar}, title = {Efficacy of different antimycotoxicosis compounds on the main immune organs and humoral immune response of broiler chickens fed on aflatoxin and/or ochratoxin contaminated diet}, journal = {Benha Veterinary Medical Journal}, volume = {33}, number = {2}, pages = {430-446}, year = {2017}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2017.30591}, abstract = {The ameliorative effects of some different antimycotoxicosis compounds (AMCs) against the adverse effects induced by intoxication of dietary aflatoxin (AF) and/or ochratoxin (OT) on the immune system performance in broiler chicks were investigated.AF (23 ppb), OT (17 ppb) and mixed doses were fed and in association with antimycotoxin feed additives; product A (combination of Eubacterium BBSH797 and Trichosporon mycotoxinivoran, plant extract, algae extract) or product B (nano-clay, seaweed extract, yeast cell wall, diatomaceous earth) from 1 day old chicks to 21 day. The intoxicated chicks treated with AMCs; product C(combination of bacterial cell wall extract, oligosaccharides, L-form bacteria extract, group of mycotoxin biotransforming enzymes, organic acid and salts, hepatic and renal tonic and vitamins)or product D in drinking water (isomalto-oligosaccharide, micronized-mannan oligosacharide, lactobacillus extract and cholestyramine) were used in the drinking water of 2 weeks old chicks after appearance of signs. Results revealed that chicks intoxicated with AF and/or OT showed significant (P<0.05) decrease in the relative weights of the main immune organs and the antibody titers against vaccines of Newcastle disease (ND) and Infectious bursal diseases (IBD) viruses, in addition to significant (P<0.05) increase in the cumulative histopathological lesion scores of thymus, bursa of Fabracius and spleen. The adverse effects of AF and/or OT on the relative weights and the histopathological lesion scores of the tested immune organs and the antibody titers against ND and IBD vaccination were significantly (P<0.05) improved with dietary supplementation of AMCs products A or B. While the treatment of the intoxicated chicks with AMCs products C or D in the drinking water showed partially and temporarily improvement in the relative weights and the histopathological lesion scores of the tested immune organs and did not improve the negative effects of AF and/or OT on the antibody titers against ND and IBD vaccines.}, keywords = {Mycotoxicosis – Broiler chicken,Immunity- immune organs}, url = {https://bvmj.journals.ekb.eg/article_30591.html}, eprint = {https://bvmj.journals.ekb.eg/article_30591_8975ce02ffcbfef7367f17f856a81f4b.pdf} } @article { author = {Hassanin, Faten and Hassan, Mohamed and Shaltout, Fahim and Shawqy, Nahla and Abd-Elhameed, Ghada}, title = {Bacteriological criteria of chicken giblets}, journal = {Benha Veterinary Medical Journal}, volume = {33}, number = {2}, pages = {447-456}, year = {2017}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2017.30592}, abstract = {A total of 50 random samples of liver and gizzard from freshly slaughtered chicken carcasses(slaughtered, plucked and eviscerated) (25 of each) were collected from local commercial retailshops in Menofia government. It is evident from the results that the mean value of aerobic platecount (APC) (cuf/g) of examined samples of chicken giblets was 4.86×104±0.92×104 in liver and7.73×104±1.68×105 in gizzard and the incidence of E.coli in the examined chicken liver and gizzardwere 20% and 28% , respectively. Also, the serologically identified E.coli isolates in the examinedsamples were O26 : H11 (4%) , O55: H7 (4%), O91: H21 (4% )and O128: H2(8%) in liver and O26 :H11 (8%) , O78(4%), O111: H2 (4% ), O119: H6(4%), O124(4% )and O126: H21(4%) in gizzard.Salmonella was isolated from 24% and 36% of liver and gizzard and incidence of the isolatedserotypes were S.Entritidis (4% and 12%), S.Infantis (4% and 4%), S.Kentucky (4% and 4%),S.Typhimurium (8% and 8%), S.Labadi 4% & S.Virchow 4% in gizzard and S.Larochelle 4% inliver only.}, keywords = {Liver,Gizzard,APC,E. coli,Salmonella}, url = {https://bvmj.journals.ekb.eg/article_30592.html}, eprint = {https://bvmj.journals.ekb.eg/article_30592_45e724f256f1dbd97b8f37893900d630.pdf} } @article { author = {Hassanin, Faten and Hassan, Mohamed and Shaltout, Fahim and Shawqy, Nahla and Abd-Elhameed, Ghada}, title = {Chemical criteria of chicken meat}, journal = {Benha Veterinary Medical Journal}, volume = {33}, number = {2}, pages = {457-464}, year = {2017}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2017.30595}, abstract = {The chemical composition of each chicken cuts-up is greatly varied from one to another. A total of50 random samples of chicken meat represented by breast and thigh (25 for each) were collectedfrom local commercial retail shops at Menoufia governorate. Each sample weighting about 100gm.The collected samples were tested for their PH values, TVN, TBA and Peroxide value. The resultsshow that the maximum values of pH in the examined chicken breast and thigh samples rangedfrom 5.7 to 5.96 and 5.65 to 5.84, respectively with mean values of 5.81 ± 0.05 and 5.73 ± 0.03,respectively. TVN value in the examined samples of chicken carcasses was ranged from 2.3 to 8.5with an average of 5.56 ± 0.24 mg% for breast and 3.8 to 10.9 with an average of 7.12 ± 0.29 mg%for thigh. TBA values (mg%) of the examined samples of chicken breast and thigh were rangedfrom 0.06 to 0.14 and 0.08 to 0.21mg%, respectively with mean values of 0.09 ± 0.01 and 0.14 ±0.01, respectively.The peroxide values in the examined samples of chicken carcasses were variedfrom 0.11 to 0.26 with a mean value of 0.19± 0.01 for breast and 0.14 to 0.39 with a mean value of0.27± 0.01 for thigh. From the previous results it could be concluded that chicken meat from localcommercial retail shops in Menoufia governorate were acceptable based on their pH values, TVN,TBA and Peroxide value as all samples were nearly within their permissible limits.}, keywords = {Breast,thigh,pH,tba,peroxide value}, url = {https://bvmj.journals.ekb.eg/article_30595.html}, eprint = {https://bvmj.journals.ekb.eg/article_30595_084f21320d2304f47c771736514e364c.pdf} } @article { author = {Abd El-Tawab, Ashraf and Hofy, Fatma and Mohamed, Sahar and Salim, Reham}, title = {Bacteriological and molecular studies on some bacterial agents from neonatal calf diarrhea}, journal = {Benha Veterinary Medical Journal}, volume = {33}, number = {2}, pages = {465-475}, year = {2017}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2017.30596}, abstract = {Neonatal Calf diarrhea(NCD) is a commonly reported disease and considered as a major cause ofeconomic loss to cattle producers. This study was done on 100 fecal swabs collected fromdiarrheic calves(0 day-2monthes) old during the period from December 2015 till January 2017 andsubjected for bacteriological ,serological and molecular investigations. The infection rate of E.coliwas 47% followed by Pseudomonas aeruginosa( 4% )and Salmonella Typhimurum( 1%). Multidrug resistant appeared in the most tested microorganisms. Serogrouping of E. coli revealed thepresence of O142 ,O55 ,O11,O27, O157,O119 ,O26 and O127 by a percentage of20.69%,17.24%,13.8%,6.9%,6.9%.6.9%,3.45% and 3.45%, respectively. Multiplex PCR wasapplied for detection of virulence genes stx1(5/10),stx2(3/10) and eae(6/10)) which detected inE.coli and stn gene in Salmonella typhimurum and also (blaVIM( 3/4), mexR(4/4)) genes forPseudomonas aeruginosa were detected}, keywords = {Calf diarrhea,E.coli,Pseudomonas aeruginosa,Salmonella typhimurum,Virulence &resistance genes}, url = {https://bvmj.journals.ekb.eg/article_30596.html}, eprint = {https://bvmj.journals.ekb.eg/article_30596_bc8487e203cc898cf3b7c2c4912e720a.pdf} } @article { author = {El-Bagoury, Gabr and El-Habbaa, Ayman and Helmy, Nashwa and El- Habashy, Rawan,}, title = {Convenience of rapid detection tests in suspecting prevalence of FMD virus in Egypt between 2016-2017}, journal = {Benha Veterinary Medical Journal}, volume = {33}, number = {2}, pages = {476-488}, year = {2017}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2017.30597}, abstract = {Egypt is endemic for Foot and mouth disease (FMD) virus with continuous long-lasting outbreaksin different provinces causing significant losses in the animal livestock. This study was designed toshow convenience of rapid detection tests in suspecting prevalence of FMD virus in Egypt duringautumn and winter 2016 and spring and summer 2017. Samples collected from clinically suspectedcattle and buffaloes at different governorates, were subjected to antigen detection ELISA and realtime reverse transcription polymerase chain reaction (rRT-PCR). Although FMD virus serotype Ois more prevalent during that period, serotypes A and SAT2 were also found in less prevalent cases.It was showed that from 2018 samples, 62 (28.44%) and 72 (33.02%) samples were positive forFMD virus (Serotypes A, O and SAT2) using antigen detection ELISA and rRT-PCR, respectively.Trials for virus isolation on BHK-21 from aseptically prepared 24 positive samples by antigendetectionELISA and rRT-PCR, revealed four isolates, three for serotype O samples and one SAT2.Finally, rRT-PCR was employed as it has a greater sensitivity over the conventional ELISA andvirus isolation for the diagnosis of FMD virus suspected samples which are not detected by theELISA or not produce a CPE in cell cultures with fast and quantitative assessment of the virus.}, keywords = {FMDV,ELISA,rRT-PCR,virus isolation}, url = {https://bvmj.journals.ekb.eg/article_30597.html}, eprint = {https://bvmj.journals.ekb.eg/article_30597_99ad417de438abbe825ff10788299e62.pdf} } @article { author = {Abd El Tawab, Ashraf and Maarouf, Ahmed and El Hofy, Fatma and El Mougy, Emad}, title = {Detection of some virulence genes in A. hydrophila and A. caviae isolated from fresh water fishes at Qalubia Governorate}, journal = {Benha Veterinary Medical Journal}, volume = {33}, number = {2}, pages = {489-503}, year = {2017}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2017.30598}, abstract = {The study was conducted on 225 diseased fish samples, 125 Nile tilapia (Oreochromis niloticus)and 100 Catfish(Claris gariepinus) , collected from different fish markets at Qalubia Governorateduring the period from January (2016) to May (2017) for detection of Aeromonas species. Thesamples were taken from apparently pathognomonic lesions in muscle, kidney, liver, intestine andspleen after clinical and postmortem examination for bacteriological examination. The resultsrevealed that, 125 Aeromonas species were isolated from the examined samples where A.hydrophila and A. caviae were identified. Accurately 114 (91.2 %) A. hydrophila strains, 63(50.4%) and 51 (40.8%) were isolated from C. gariepinus and O. niloticus fishes respectively.Meanwhile, 11(8.8 %) A. caviae strains, 7 (5.6%) and 4 (3.2%) from C. garicpinus and O. niloticusfishes respectively. Further PCR results for virulence genes in isolated Aeromonas strains indicatedthat, aero gene was detected in 9 out of 10 A .hydrophila studied strains and in 3 out of 6 A. caviae ;hly gene in 7 out of 10 A .hydrophila and in 2 out of 6 A. caviae; Ahcytoen gene in 6 out of 10 A.hydrophila and in 1 out of 6 A. caviae ; act gene in 6 out of 10 A .hydrophila and in 3 out of 6 A.caviae and ast gene in 7 out of 10 A .hydrophila and in 3 out of 6 A. caviae studied strains. Finally,the production of a wide array of virulence factors by isolated strains is indicative of their potentialto cause diseases in fishes and humans.}, keywords = {fish,Bacteriological,aeromonas,Virulence genes}, url = {https://bvmj.journals.ekb.eg/article_30598.html}, eprint = {https://bvmj.journals.ekb.eg/article_30598_23c61faf1959efbe4563307aacedad1a.pdf} } @article { author = {Hussein, Samy and El Senosi, Yakout and Mansour, Mogda and Hassan, Marwa}, title = {Role of antioxidant and anti-inflammatory of Ginger (Zingiber officinal Roscoe) against metalaxyl induced oxidative stress in rats.}, journal = {Benha Veterinary Medical Journal}, volume = {33}, number = {2}, pages = {504-516}, year = {2017}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2017.30599}, abstract = {The purpose of this study was carried to evaluate the protective and anti-inflammatory effect ofginger against metalaxyl toxicity induced liver damage and oxidative stress in rats. Forty two malealbino rats were divided into three equal groups. Group I (normal group): rats administered distilledwater. Group II (metalaxyl exposed group): rats received metalaxyl 1/10 LD50 (130 mg/kg b.wt)orally three times per weeks for 8 weeks. Group III (metalaxyl + ginger treated group): rats receivedmetalaxyl (130 mg/kg b.wt) and treated with ginger (100 mg/kg b.wt/day/orally). The obtainedresults showed significant increase in serum hepatic function enzymes (ALT, AST and ALP)activities, hepatic tissue L-MDA levels and inflammatory biomarkers (MPO and IL-6) in metalaxylexposed rats. However, activities of liver tissue antioxidant enzymes (SOD, CAT) and GSHconcentration were markedly decreased. Administration of ginger with metalaxyl exposed ratscaused significant improvement of all previous parameters towards its normal ranges. These resultssuggested that, ginger treatment may have a protective effect against liver damage and oxidativestress in rats through free radical scavenging and anti-inflammatory activity as well as regeneratingendogenous antioxidant defense system mechanisms.}, keywords = {Metalaxyl,Ginger,Oxidative Stress,Rats}, url = {https://bvmj.journals.ekb.eg/article_30599.html}, eprint = {https://bvmj.journals.ekb.eg/article_30599_f88105a08f83f7c3c8cda861cc0869e0.pdf} } @article { author = {Hussein, Samy and El Senosi, Yakout and Mansour, Mogda and Hassan, Marwa}, title = {Potential protective effects of Quercetin on metalaxyl-induced oxidative stress, impaired liver functions and hepatotoxicity in rat}, journal = {Benha Veterinary Medical Journal}, volume = {33}, number = {2}, pages = {517-532}, year = {2017}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2017.30600}, abstract = {The purpose of this study was to investigate the protective and anti-inflammatory influence ofquercetin against metalaxyl fungicide induced liver toxicity and oxidative stress in rats. Forty twomale albino rats were divided into three equal groups. Group I (normal group): rats administereddistilled water. Group II (metalaxyl exposed group): rats received metalaxyl 1/10 LD50 (130 mg/kgb.wt) orally three times per weeks for 8 weeks. Group III (metalaxyl + quercetin treated group): ratsreceived metalaxyl (130 mg/kg b.wt) and treated with quercetin (15 mg/kg b.wt/day/i.p). Theobtained results showed significant increase in serum liver marker enzymes (ALT, AST and ALP)activities, liver tissue L-MDA and inflammatory markers (MPO and IL-6) levels in metalaxylexposed rats. However, activities of liver tissue antioxidant enzymes (SOD, CAT) and GSH levelswere markedly decreased. Administration of quercetin with metalaxyl exposed rats causedsignificant improvement of all previous parameters towards its normal ranges. These resultssuggested that, quercetin treatment may have a protective effect against metalaxyl fungicideinduced liver damage and oxidative stress in rats via anti-inflammatory activity and free radicalscavenging as well as regenerating endogenous antioxidant defense system mechanisms.}, keywords = {Metalaxyl,Quercetin,antioxidant,inflammatory markers}, url = {https://bvmj.journals.ekb.eg/article_30600.html}, eprint = {https://bvmj.journals.ekb.eg/article_30600_370832fa99ccd7938c61373eee1d8f24.pdf} }