@article { author = {Abd EL-Tawab, Ashraf and Ammar, Ahmed and Hamouda, Ahmed and El-Hofy, Fatma and Elgamal, Abdelrahman}, title = {Synergistic antimicrobial activity of black pepper extract with some antibiotics combination on Escherichia coli isolated from chickens}, journal = {Benha Veterinary Medical Journal}, volume = {32}, number = {1}, pages = {1-6}, year = {2017}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2017.31104}, abstract = {Antimicrobial activity of methanol piper nigrum extract in combination with some antibiotics was investigated againstEscherichia coli isolated from chicken samples. The in-vitro antimicrobial sensitivity pattern of these isolates was doneand the intermediate isolates were checked for combination with methanol piper nigrum extract after detecting theirantimicrobial activities against these isolates by both disc diffusion test and MIC and the obtained data showed thatantimicrobial activities of amoxicillin, doxycycline, gentamicin, erythromycin and cefotaxime were enhanced incombination with methanol piper nigrum extract. The obtained data showed that enhancement by both disc diffusion testand MIC for methanol piper nigrum extract and each of the mentioned antibiotics in decimal assay of additivity ratio was(0.5 for amoxicillin, cefotaxime and 0.5 for extract and 0.4 for doxycycline, gentamicin, and erythromycin and 0.6 forextract) against E. coli isolates.}, keywords = {Antimicrobial combination,black pepper extract,Escherichia coli,antibiotics,Synergism}, url = {https://bvmj.journals.ekb.eg/article_31104.html}, eprint = {https://bvmj.journals.ekb.eg/article_31104_a7fdd34d721ef897ade3deaffd7d3d6c.pdf} } @article { author = {Abd EL-Tawab, Ashraf and Ammar, Ahmed and Hamouda, Ahmed and El-Hofy, Fatma and Elgamal, Abdelrahman}, title = {Synergistic antimicrobial combination with cumin extract and some antibiotics on Staphylococcus aureus from chickens}, journal = {Benha Veterinary Medical Journal}, volume = {32}, number = {1}, pages = {7-12}, year = {2017}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2017.31105}, abstract = {In the present study, antimicrobial activity of methanol Cuminum cyminum extract (50mg/ml) in combination with someantibiotics were investigated against Staphylococcus aureus isolates. The antimicrobial sensitivity pattern of theseisolates in vitro was done and the intermediate isolates were checked for combination with methanol Cuminum cyminumextract after detecting their antimicrobial activities against these isolates by both disc diffusion test and MIC and theresults showed that the antimicrobial activities of rifampicin, enrofloxacin, doxycycline, gentamicin, and erythromycinwere enhanced in combination with methanol Cuminum cyminum extract the obtained data showed that enhancement byboth disc diffusion test and MIC for methanol Cuminum cyminum extract and each of mentioned antibiotics in decimalassay of additivity ratio (0.5 for rifampicin and erythromycin and 0.5 for extract ,0.7 for enrofloxacin, and 0.3 forextract,0.6 for doxycycline, gentamicin and 0.4 for extract) against S.aureus isolates}, keywords = {Antimicrobial combination,Staphylococcus aureus,cumin extract,antibiotics,Synergism}, url = {https://bvmj.journals.ekb.eg/article_31105.html}, eprint = {https://bvmj.journals.ekb.eg/article_31105_60ee9c367408cd556d42e0ad6b25294a.pdf} } @article { author = {Shaltout, Fahim and Farouk, Mohammed and Ibrahim, Hosam and Afifi, Mostafa}, title = {Incidence of Coliform and Staphylococcus aureus in ready to eat fast foods}, journal = {Benha Veterinary Medical Journal}, volume = {32}, number = {1}, pages = {13-17}, year = {2017}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2017.31106}, abstract = {A total of 100 random samples of ready- to – eat sandwiches of beef products of kofta , liver, shawerma, and sausageproducts (25 samples of each ) were collected from different fast food services in different districts at Kaliobia governorateto be examined bacteriologically for detection of Coliform and Staphylococcus aureus microorganisms. The obtainedresults in the present study indicated that the mean value of coliform counts (cfu/g) in the examined samples of ready- to- eat meat products were 2.5 x 103 ± 0.74×103 for beef kofta, 8.85x102 ±1.92x 102 for beef shawerma, 8 x103 ± 1.65 x 103for beef sausage, 9.0 x 104 ± 2.30 x 104 for beef liver, furthermore the coliform were detected in 60% of beef kofta, 40%of beef shawerma, 52% of beef sausage and 80% of beef liver. The obtained results in the present study indicated that thestaphylococcus aureus was detected in 32% of beef kofta, 44% of beef liver, 8% of beef shawerma and 16% of beafsausage .The obtained results in the present study indicated that the ready- to-eat liver sandwiches were morecontaminated with Staphylococcus aureus as compared with those of kofta, shawerma and sausage. The examinedsamples of ready-to-eat liver sandwiches showed high incidence of coliform than those obtained by kofta, sausage andshawerma.}, keywords = {ready- to – eat sandwiches,Coliform,Staphylococcus aureus,kofta,Liver,shawerma,sausage}, url = {https://bvmj.journals.ekb.eg/article_31106.html}, eprint = {https://bvmj.journals.ekb.eg/article_31106_78f2b5435cae236b717ff66fab61d98a.pdf} } @article { author = {Shaltout, Fahim and Farouk, Mohammed and Ibrahim, Hosam and Afifi, Mostafa}, title = {Incidence of E. coli and Salmonellae in ready to eat fast foods}, journal = {Benha Veterinary Medical Journal}, volume = {32}, number = {1}, pages = {18-22}, year = {2017}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2017.31107}, abstract = {A total of 100 random samples of ready- to – eat sandwiches of beef products represented by kofta, liver, shawerma andsausage products (25 samples of each) were collected from different fast food services in different districts at kaliobiaGovernorate to be examined bacteriologically for detection of Salmonellae and Escherichia coli. The perecentages ofSalmonellae in the examined samples of kofta, liver, shawerma and sausage products were 32%, 60%, 8% and 40%,respectively. The obtained results indicated that the incidence of E. coli was 20%, 8% and 32% and 40% in the examinedkofta, shawerma, sausage and liver samples, respectively}, keywords = {ready- to – eat sandwiches,E. coli,Salmonellae,kofta,Liver,shawerma,sausage}, url = {https://bvmj.journals.ekb.eg/article_31107.html}, eprint = {https://bvmj.journals.ekb.eg/article_31107_c9c949ba23f4d5458186e1d2a300c47c.pdf} } @article { author = {Ibrahim, Hemmat and Amin, Reham and Eleiwa, Nesreen and Ahmed, Noha}, title = {Estimation of some biogenic amines on chicken meat products}, journal = {Benha Veterinary Medical Journal}, volume = {32}, number = {1}, pages = {23-28}, year = {2017}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2017.31108}, abstract = {Sixty random samples of raw thigh & breast, half cooked chicken shawerma & shiesh tawook, fully cooked chickenshawerma and fully cooked chicken shiesh tawook (10 of each) were collected from different markets in Gharbiagovernorates and analyzed for biogenic amines by HPLC. The obtained results indicated that the mean values ofhistamine, cadaverine and putrescine (mg/100g) were 10.88 ± 0.91, 12.65 ± 1.10 and 4.54 ± 0.32 for raw thigh, 4.54 ±0.32, 9.86 ± 0.87 and 9.86 ± 0.87 for raw breast 16.56 ± 1.24, 19.81 ± 1.52, and 11.41 ± 0.96 for half cooked shawerma,12.29 ± 0.97, 16.20 ± 1.08 and 9.98 ± 1.08 for half cooked shiesh tawook, 12.93 ± 1.03, 16.69 ± 1.33 and 8.01 ± 0.63 forfully cooked shawerma and 11.68 ± 0.97, 11.91 ± 0.97 and 7.47 ± 0.50 respectively for fully cooked shiesh tawook.According to the permissible limits recommended by EOS (2005) 10%, 30%, 20%, 20% and 20% of the examinedsamples of raw thigh, half cooked shawerma, half cooked shiesh tawook, fully cooked shawerma and fully cooked shieshtawook were unaccepted. All raw breast samples were accepted as a result of their histamine content. While, 20%, 40%,30%, 30% and 20% of the examined samples raw thigh, half cooked shawerma, half cooked shiesh tawook, fully cookedshawerma and fully cooked shiesh tawook were unaccepted. Moreover, raw breast samples were accepted as result oftheir cadaverine content respectively. Accurately 10% of half cooked shawerma were unaccepted while all examinedsamples were accepted as a result of putrescine content}, keywords = {biogenic amines,Chicken Meat Products,histamine,cadaverine,putrescine}, url = {https://bvmj.journals.ekb.eg/article_31108.html}, eprint = {https://bvmj.journals.ekb.eg/article_31108_5b634670d823bf60a9143bb1f68b3490.pdf} } @article { author = {Gharib, Ahlam and Sleim, Mokhtar and Hegazy, Mohamed and M., Abdel-wahab and Adly, Sara}, title = {Bacteriological and molecular studies of garlic effect on some virulence genes of Escherichia coli of chicken origin}, journal = {Benha Veterinary Medical Journal}, volume = {32}, number = {1}, pages = {29-40}, year = {2017}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2017.31109}, abstract = {Avian colibacillosis is the most significant infectious bacterial disease of poultry worldwide. Many antimicrobial-resistantEscherichia coli pathogens are increased due to drug overusing in veterinary medicine and animals feed. The objective ofthis study was to observe the in vitro inhibitory effect of garlic (Allium sativum) as alternative natural agent againstEscherichia coli isolates and their virulence genes expression. The antimicrobial effects of aqueous and ethanolic garlicextracts against multidrug-resistant (MDR) E. coli isolates were studied using agar well-diffusion method as well as theminimum inhibitory concentration (MIC) of garlic and consequently their subinhibitory concentration (SIC) (theconcentration of garlic that did not inhibit E. coli growth after 24 hr. incubation at 37ºC) were calculated by a modificationof broth macrodilution method. The E. coli virulence was assessed via mRNA expression of their genes such as increasedserum survival (iss), verotoxine (vt2e) and intimin (eaeA) before and after garlic treatment using reverse transcriptase realtime PCR. One hundred and thirteen (46.12%) of 245 isolates were identified as E. coli that were highly recorded inintestinal samples as 64.60% followed by liver 24.77% then heart 10.6%. The most chemotherapeutic resistant 14 E. coliisolates showed high garlic susceptibility rates mainly for aqueous extract and had nine different serotypes, the mostpredominant one was O146 of 21.42%. The complete inhibition of and downregulating of eaeA and vt2e genes expressionwere proved respectively in the E. coli treated isolates with garlic SIC (1%). In conclusion, the garlic supplementationthrough feed can reduce infection by E. coli via decrescent their toxin production and may be the adhesion ability to theintestinal mucosa of the host either animal or human}, keywords = {Garlic (Allium sativum)-MIC– E. coli- virulence gene}, url = {https://bvmj.journals.ekb.eg/article_31109.html}, eprint = {https://bvmj.journals.ekb.eg/article_31109_c21aba465769d09e885206c9bd4f4f97.pdf} } @article { author = {Salman, Ahmed and Abd El-Aleem, Ibrahim and Abd-El Rahman, Ahmed and Elhusseini, Tarek and El-Hadary, Abd Allah}, title = {Protective impacts of Cupressus sempervirens leaves extracts against paracetamol hepatotoxicity}, journal = {Benha Veterinary Medical Journal}, volume = {32}, number = {1}, pages = {41-49}, year = {2017}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2017.31110}, abstract = {The main objective of this study is to evaluate the antioxidant activity of Cupressus sempervirens leaves extract invitroand rat model in which paracetamol was used to induce hepatic damage. The antioxidant and anti-quorum sensingactivities of eight extracts were studied. Total phenolic compounds and scavenging radical effect on 2, 2-diphenylpicrylhydrazyl (DPPH) were investigated, (the specific phenolic and flavonoid composition quantification forhydrolyzed ethyl acetate fraction pH 4 were performed by HPLC E-vanilic, Alpha-coumaric, Salicylic and Ferulic werefound as the major phenols. Narengin, Hisperidin, Quercetrin and Hispertin were found as the major flavonoids.Biological experiment was carried out for thirty days. Results revealed that a significant increase in serum ALT, AST,ALP, Total bilirubin, TG, Total cholesterol, (LDLC) and nitric oxide in rats treated with paracetamol. Howeveradministration of Cupressus sempervirens in paracetamol induced liver toxicity in rats exhibited a significant decreasein all mentioned parameters on the other hand a significant decrease in serum total protein albumin concentration,(HDLC), Catalase, glutathione peroxidase (GPX), Glutathione –S-transferase (GST) and Glutathione reduced wereobserved in paracetamol induced hepatic toxicity in rats when compared with control normal group. MeanwhileCupressus sempervirens leaves resulted in significant in all mentioned parameter and when enhanced the activity ofantioxidant enzymes in liver tissue. It could be concluded that, inhibition of peroxidation, inflammation and oxidativestress and enhanced antioxidant status in rat liver tissue by Cupressus sempervirens suggest the potential efficacy ofCupressus sempervirens as an addition Hepatoprotective, anti-inflammatory and anti-hepatotoxic agent in treatment ofliver toxicity}, keywords = {Cupressus sempervirens leaves,antioxidant,Hepatoprotective,Paracetamol}, url = {https://bvmj.journals.ekb.eg/article_31110.html}, eprint = {https://bvmj.journals.ekb.eg/article_31110_339bb4dd477aa06b482092eee4d4a882.pdf} } @article { author = {S., Hassanein and A., Shaltout and A., El Shater and A., ElKewaiey and M., El-Minisy}, title = {Bacterial profile of bovine carcasses at abattoir}, journal = {Benha Veterinary Medical Journal}, volume = {32}, number = {1}, pages = {50-53}, year = {2017}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2017.31111}, abstract = {A grand total of 180 swabs representing 60 bovine carcasses were collected under aseptic conditions from different parts(hind quarter, abdomen and fore quarter) of the carcass surface at 2 Basatin abattoir halls in summer. Collected sampleswere subjected to bacteriological examination and the results of APC in the following region A1, A2, A3, B1, B2, B3 inhall 1 were 3.8 x104 ± 3.4x 10, 3.5 x104 ± 2.1 x103, 4.7 x104 ± 3.1 x103, 3.3 x104 ± 1.1 x103, 3.3 x104 ± 4.4 x103, 4.4 x104± 4.3 x103. Concerning hall 2, such results were 4.6 x104 ± 9.9 x103, 5.7 x104 ± 9.2 x103, 4 x104 ± 2.7 x103, 6.4 x104 ±3.9 x103, 3.3 x104 ± 4.6 x103 and 6 x104 ± 4.8 x103 respectively. On the other hand, Salmonella Typhimurium wasisolated from the carcass swabs at a percentage 2.75% from A1, A2, B2, B3 parts. The analysis was carried out and therecommendations showed that the Salmonella Typhimurium antigenic structure acc. To serological identification wassomatic (O) antigen 1, 4, (5), 12 and flagellar (H) antigen phase 1:1, phase 2:2.}, keywords = {Bovine carcass,Salmonella,abattoir,bacterial count,meat contamination}, url = {https://bvmj.journals.ekb.eg/article_31111.html}, eprint = {https://bvmj.journals.ekb.eg/article_31111_5d75686fb3d3536d0a00bc3a9e22d3d7.pdf} } @article { author = {Elsisy, Suzan and Ali, Ali and Ibrahim, Hemmat and Hassan, Mohamed}, title = {Demonstration of Lead and Cadmium residues in Street vended foods}, journal = {Benha Veterinary Medical Journal}, volume = {32}, number = {1}, pages = {54-58}, year = {2017}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2017.31112}, abstract = {One hundred and thirty-five random samples of street vended foods represented by kofta, hawawshi and liver (45 of each)were collected from Benha (15 of each product), Tukh (15 of each product) and Moshtohor (15 of each product) inKalyobia governorate for determination of their content of lead and cadmium. The obtained results recorded that theoccurrence of cadmium in Benha city were 26.67, 40.00 and 46.67% in the kofta, hawawshi and liver samples,respectively. In Tukh center were 26.67, 46.67 and 60.00% in the kofta, hawawshi and liver samples, respectively.Whereas in Moshtohor village were 40.00, 53.33 and 73.33% in the kofta, hawawshi and liver samples, respectively. Inaddition, our results showed that the percentage of occurrence of lead in Benha city were 20.00, 60.00 and 66.67 % in thekofta, hawawshi and liver samples, respectively. In Tukh center were 33.33, 40.00 and 53.33 % in the kofta, hawawshiand liver samples, respectively. Whereas in Moshtohor village were 20.00, 33.33 and 40.00 % in the kofta, hawawshi andliver samples, respectively. It could be inferred that regarding the products contamination, the highest cadmiumcontamination was in liver followed by hawawshi then kofta. Whereas kofta showed the highest lead contaminationfollowed by hawawishi then liver. Regarding the locality, Moshtohor represented the highest contamination of bothcadmium and lead followed by Tukh then Benha city}, keywords = {Lead,cadmium,Liver,street vendors,meat products}, url = {https://bvmj.journals.ekb.eg/article_31112.html}, eprint = {https://bvmj.journals.ekb.eg/article_31112_6b778af3cb6512097bf4154194507931.pdf} } @article { author = {Ali, Hussien and Mahmoud, Omnia and Hussein, Mohammed and Mohammed, Mariam}, title = {Biochemical effect of cranberry extract on experimentally induced myocardial infarction}, journal = {Benha Veterinary Medical Journal}, volume = {32}, number = {1}, pages = {59-66}, year = {2017}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2017.31113}, abstract = {Myocardial infarction (MI) is characterized by an inequity of coronary blood supply and demand, which results inmyocardial ischemic injury and damages the cardiomyocytes. Isoproterenol (ISO) is a synthetic catecholamine causetoxicity leading to severe stress in the myocardium of experimental animals. The aim of the present article is to investigatecardioprotective activity of cranberry extract against ISO-induced cardiotoxicity in adult rats. Oral administration ofcranberry extract at a concentration of 75 and 150 mg/kg b.wt. daily for 28 days showed a significant protection againstinducedalteration in plasma total cholesterol (TC), triacyclglycerols (TG), high density lipoprotein (HDL-C), low densitylipoprotein (LDL-C), tumor necrosis factor-alpha (TNF-α) and nitric oxide (NO) as well as cardiac superoxide dismutase(SOD), glutathione peroxidase (GPx), reduced glutathione (GSH), catalase (CAT) levels. In addition, cranberry extractreduced plasma Creatine kinase-MB (CK-MB), lactate dehydrogenase (LDH) and aspartate aminotransferase (AST) aswell as cardiac thiobarbituric acid reactive substances (TBARS) levels (MDA) as compared to control group. Inconclusion, cranberry extract renders resiliency against isoproterenol cardiotoxicity due to its antioxidant and free radicalscavenging activity}, keywords = {Cranberry extract,Isoproterenol,myocardial infarction,antioxidant}, url = {https://bvmj.journals.ekb.eg/article_31113.html}, eprint = {https://bvmj.journals.ekb.eg/article_31113_9407ead6299a6b0ee2c87337b83d46fc.pdf} } @article { author = {Hussein, Samy and Abdel-Magid, Afaf and Fareed, Fareed}, title = {Biochemical effect of resveratrol on Lipids profile and hepatic oxidative stress in experimentally induced obesity in female rats}, journal = {Benha Veterinary Medical Journal}, volume = {32}, number = {1}, pages = {67-74}, year = {2017}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2017.31114}, abstract = {This study was designed to evaluate the effect of prolonged intake of resveratrol on lipid metabolism, liver enzymes andL-malondialdehyde(L-MDA) in obesity -induced in female rats by feeding high fat diet. Ninety female albino rats weredivided into six equal groups of 15 rats each. Group Ι: rats fed normal control diet all over the periods of experiment (for12 weeks). Group II: rats fed the control normal diet and administered resveratrol orally (0.1 mg/kg b.wt., orally) for 6weeks. Group III: rats received the control normal diet and received resveratrol once daily (0.5 mg/kg b.wt., orally) for 6weeks. Group IV: rats fed high fat diet (HFD) and received no drugs all over the periods of experiment (for 12 weeks).Group V: rats received HFD and administered resveratrol (0.1 mg/kg b.wt./day, orally) for 6 weeks. Group VI: rats fedHFD and administered resveratrol (0.5 mg/kg b.wt./day, orally). Blood samples were collected from all rats at 2, 4 and 6weeks from the onset of resveratrol administration for determination of serum total cholesterol(TC), Triacylglycerols(TAG), high density lipoprotein cholesterol (HDL-c), low density lipoprotein cholesterol (LDL-c), very low densitylipoprotein cholesterol (VLDL-c), alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkalinephosphatase (ALP), and erythrocyte L-malondialdehyde (L-MDA).The obtained results showed, significant elevation ofserum TC, TAG, LDL-c, VLDL-c, ALP,AST, ALT, L-MDA in obese rats as compared to rats fed normal control diet.While administration of resveratrol to HFD-fed rats tended to improve hepatic oxidative stress and dyslipidemia throughimproving total cholesterol and triacylglycerols and liver markers enzyme activities. These results suggest that, resveratrolis effective in improving the obesity and its associated many important complications such as diabetes mellitus andcoronary heart disease and liver disease}, keywords = {Resveratrol,Obesity,Lipids Profile,liver enzymes,L-malondialdehyde}, url = {https://bvmj.journals.ekb.eg/article_31114.html}, eprint = {https://bvmj.journals.ekb.eg/article_31114_1a0cc4bb23c9aab29b10cf3dfed4e71c.pdf} } @article { author = {A., Hassan and A., El- Shater and A., Waly}, title = {Histamine as biogenic amine residue in imported frozen fish}, journal = {Benha Veterinary Medical Journal}, volume = {32}, number = {1}, pages = {75-78}, year = {2017}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2017.31115}, abstract = {A total of ninety random samples of imported frozen fish represented by Mackerel, Herring and Saurus (30 of each) fromtwo different countries namely A and B (15 samples of each) were collected from different fish markets in Menoufiyagovernorate. Each sample was weighed about 150 g and stored at -18oC. The collected samples were packed separatelyin sterile polyethylene bag and transferred directly to the laboratory with minimum delay for determination of their toxicresidues. The obtained results recorded that the occurrence of histamine in both country (A) and in country (B) were100% in the Mackerel, Herring and Saurus samples. The mean value of histamine in the country (A) samples were 38.14± 2.93, 31.67 ± 2.15 and 19.05 ± 1.68 mg/kg in the Mackerel, Herring and Saurus, respectively. The average concentrationof histamine in country (B) samples were 33.96 ± 2.45, 24.51 ± 1.72 and 12.83 ± 1.19 mg/kg in the Mackerel, Herringand Saurus, respectively. It could be inferred that regarding the products contamination, the highest histaminecontamination was in mackerel followed by herring then saurus. Regarding the locality, country (A) represented thehighest contamination of histamine followed by country (B)}, keywords = {Mackerel,Herring,Saurus,histamine}, url = {https://bvmj.journals.ekb.eg/article_31115.html}, eprint = {https://bvmj.journals.ekb.eg/article_31115_408167c45213878cd874ef538bf777f9.pdf} } @article { author = {A., Hassan and A., El- Shater and A., Waly}, title = {Demonstration of Cadmium and Lead residues in Imported Frozen Fish}, journal = {Benha Veterinary Medical Journal}, volume = {32}, number = {1}, pages = {79-82}, year = {2017}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2017.31116}, abstract = {A total of ninty random samples of imported frozen fish represented by Mackerel, Herring and Saurus (30 of each)imported from two different countries namely A and B (15 samples of each fish species for each country) were collectedfrom different fish markets in Menoufiya governorate. The obtained results recorded that the occurrence of cadmium incountry (A) were53.33, 40.00 and 33.33% in the Mackerel, Herring and Saurus samples, respectively. In country (B) thecadmium occurrence was 40, 26.67 and 20.00% in the Mackerel, Herring and Saurus samples, respectively. In addition,our results recorded that the percentage of occurrence of lead in country (A) were 66.67, 66.67and 53.33% in theMackerel, Herring and Saurus samples, respectively; while in country (B) were 60, 46.67 and 40 % in the Mackerel,Herring and Saurus samples, respectively. It could be inferred that regarding the products contamination, the highestcadmium and lead contamination was in mackerel followed by herring then saurus. Regarding the locality, country (A)represented the highest contamination of both cadmium and lead followed by country (B).}, keywords = {Mackerel,Herring,Saurus,cadmium and lead}, url = {https://bvmj.journals.ekb.eg/article_31116.html}, eprint = {https://bvmj.journals.ekb.eg/article_31116_988136b55bf7ec9b96cf8b1988090d43.pdf} } @article { author = {Yurdakul, Özen and Kırdar, Seval and Doğru, Aylin and Keyvan, Erhan}, title = {Survival of Escherichia coli O157:H7 during processing and ripening period of Keş Cheese}, journal = {Benha Veterinary Medical Journal}, volume = {32}, number = {1}, pages = {83-88}, year = {2017}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2017.31117}, abstract = {In this study the survival of Escherichia coli O157:H7 during the production process and ripening period of Keş cheesewas investigated. Three vats containing equal amounts of raw milk were inoculated with 102, 104, 106 cfu mL-1 E. coliO157:H7, respectively andone vat remained uninoculated. Keş cheese was produced from both inoculated anduninoculated milks with the traditional method of the region. Cheese samples ripened at 6°C for 90 days and E. coliO157:H7 enumarations were done from uninoculated milk, inoculated milks, curds and different stages of ripening period.Most Probable Number technique was used for the analysis of E. coli O157:H7. Classical culture methods were used forthe detection of aerobic mesophilic bacteria, lactococci and coliform bacteria. Also, control cheese was analysed for dry matter, salt content in dry matter and pH value. Elimination times were 45 days for 102 cfu mL-1, 75 days for 104 cfu mL-1, and 90 days for 106 cfu mL-1 of E. coli O157:H7. The final dry matter, salt content in dry matter and pH value were 82.52 %, 3.16 % and 4.58, respectively. Consequently, Keş cheese should be ripened at least 90 days in order to beconsidered as safe for public health.}, keywords = {Elimination times,Escherichia coli O157:H7,Keş cheese,traditional cheese,dairy producs}, url = {https://bvmj.journals.ekb.eg/article_31117.html}, eprint = {https://bvmj.journals.ekb.eg/article_31117_194774fa2ef921930908fa0ece9697e9.pdf} } @article { author = {S., Saad and A., Hassan and Abou El-Roos, Nahla and H., Gaafar}, title = {E. coli strains producing Shiga toxin in cattle carcasses at abattoir level}, journal = {Benha Veterinary Medical Journal}, volume = {32}, number = {1}, pages = {89-93}, year = {2017}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2017.31120}, abstract = {A total of one hundred random swab samples were collected from the cattle carcasses slaughtered at four differentabattoirs located in Menufia governorate namely A, B, C and D (25 of each). The sampling site was randomly taken fromeach carcass inside the abattoir. The collected samples were examined for presence of E. coli and their virulence genesusing PCR technique. Incidence of E. coli isolated from the examined cattle swabs samples taken from abattoir A was E.coli (O55: H7, O111: H2, O126 and O114: H21) 4 % for each, E. coli (O111: H2) was 4 % and E. coli (O126) 4%. Whilein case of abattoir B E. coli (O86, O124 and O126) 4 % for all and E. coli (O119: H4 and O26: H11) 8% for both. On theother hand, results of abattoir C was E. coli (O111: H2 8% and O26: H11 12%), E. coli (O86, O114: H21, O124 andO142) 4% of each. But in case of abattoir D was E. coli O26: H11 16%, E. coli (O111: H2 O55: H7 and O126) 8% foreach, E. coli (O44: H18 and O125: H21) 4% for both. We found also that Shiga- toxin 1 gene (stx1) virulence gene waspresent in O26: H11, O44: H18, O55: H7, O111: H2, O119: H4, O126 and O128: H2. On the other hand, Shiga- toxin 2gene (stx2) virulence gene was present in O26: H11, O44: H18, O86, O111: H2, O114: H21, O119: H4, O125: H21 andO142. While intimin gene (eae) virulence gene was present in O26: H11 and O111: H2. Also haemolysin gene (hylA)gene was present in O26: H11, O55: H7 and O111: H2.}, keywords = {Cattle carcasses,E. coli,Virulence genes,Shiga- toxin}, url = {https://bvmj.journals.ekb.eg/article_31120.html}, eprint = {https://bvmj.journals.ekb.eg/article_31120_9b0608cf401cb0deb4ae181912e3e279.pdf} } @article { author = {S., Saad and A., Hassan and Abou El-Roos, Nahla and H., Gaafar}, title = {Staphylococcal contamination of cattle carcasses with particular reference to Staph. aureus enterotoxins}, journal = {Benha Veterinary Medical Journal}, volume = {32}, number = {1}, pages = {94-98}, year = {2017}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2017.31121}, abstract = {A total of one hundred random swab samples were collected from the cattle carcasses slaughtered at four differentabattoirs located in Menufia governorate namely A, B, C and D (25 of each). The sampling site was randomly taken fromeach carcass inside the abattoir. Mean values of total Staphylococci count (cfu/cm2) of the tested cattle carcass swabsamples obtained from abattoir A, B, C and D were 5.79×102 ± 1.14×102, 1.01×103 ± 0.37×103, 4.58×103 ± 0.92×103 and6.94×103 ± 1.21×103, respectively. Isolates serotypes were Staph. Aureus (12%), Staph. Epidermidis (4%), Staph.Intermedius (0%), Staph. Saprophyticus (0%), Staph. Capitis (0%) and Micrococcus spps (4%) in abattoir A. While thefore mentioned serotypes were recorded in abattoir B in percentage of (20%), (8%), (0%), (4%), (0%), (0%), respectively.On the other hand, the mentioned serotypes were recorded in abattoir C in percentage of (24%), (0%), (0%), (4%), (0%),(8%). Finally, in case of abattoir D the serotypes mentioned above found in percentage of (40%), (8%), (4%), (0%), (4%),(0%), respectively. Enterotoxin type A was produced by 2 strains of Staph. aureus at age of 13.33%. While enterotoxintype B, D, A+C and A+D were produced by single strain of Staph. aureus for each at age of 6.67%. On the other hand,enterotoxin type C is produced by 3 strains of Staph. aureus at age of 20%. Finally, there were 8 strains of Staph. aureuswas not capable of producing enterotoxin at age of 53.33%.}, keywords = {Cattle carcasses,Abattoirs,Staphylococci count,Staph. aureus virulence genes,enterotoxin}, url = {https://bvmj.journals.ekb.eg/article_31121.html}, eprint = {https://bvmj.journals.ekb.eg/article_31121_1c5a6608bd85d393defa1c21e0adbbe4.pdf} } @article { author = {Abdel Mageid, Afaf and Abou Salem, Mohamed and Salaam, Nancy}, title = {1Biochemistry Department, Faculty of Vet. Med. Benha Univ., Egypt.}, journal = {Benha Veterinary Medical Journal}, volume = {32}, number = {1}, pages = {99-103}, year = {2017}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2017.31160}, abstract = {The present study was to determine the effect of aged garlic extract (AGE) on alleviating diabetic cardiomyopathy (DCM)in rats. An experimental diabetic rat model was induced by a single dose (60mg/kg body weight) of I.P injection ofstreptozoticin (STZ). AGE was orally administrated at a dose of 500 mg/kg body weight. Metabolic profiles include serumglucose, insulin, total cholesterol and triacylglycerols and myocardial enzymes activities as creatine kinase-isoenzyme(CK-MB), lactate dehydrogenase (LDH) and aspartate aminotransferase (AST) were determined. The obtained resultsindicated that administration of AGE increased the body weight and insulin concentration, meanwhile, significantdecrease in serum glucose concentration, CK-MB and LDH activities when compared with untreated induced diabeticgroup. Total cholesterol, triacylglycerols and AST showed non-significant changes in serum values in AGE treateddiabetic rats in comparing with STZ-induced diabetic group. From the obtained results it could be concluded that, AGEhas great potential therapeutic effect in the diabetic cardiomyopathy and other complications of coronary heart disease}, keywords = {Diabetic cardiovascular diseases,aged garlic extract,myocardial enzymes}, url = {https://bvmj.journals.ekb.eg/article_31160.html}, eprint = {https://bvmj.journals.ekb.eg/article_31160_130d184143cc081463cd7d7d2c87176c.pdf} } @article { author = {GabAllh, Mahmoud and El-mashad, AbdEl-baset and Amin, Aziza and Darweish, Marwa}, title = {Pathological studies on effects of ivermectin on male and female rabbits}, journal = {Benha Veterinary Medical Journal}, volume = {32}, number = {1}, pages = {104-112}, year = {2017}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2017.31162}, abstract = {Ivermectin considered natural fermentation product derived from soil bacterium streptomyces avermitis and itis abroad spectrum insecticide, acaricide and anthelimintic. This studies carried out in order to investigate thehistopathological effects of therapeutic and double therapeutic dose of ivermectin on mature male rabbits aswell as effect of therapeutic dose of same drug on pregnant female rabbits. Forty mature rabbits were used inthis study (30 males and 10 pregnant female). Male rabbits received therapeutic and double therapeutic dosesof ivermectin, while female rabbits received therapeutic dose. The histopathological results revealed mild tomoderate reversible degenerative changes in examined organs of male rabbits received ivermectin weekly for4weeks meanwhile, this degeneration become more severe and extended to complete necrosis in some organsafter longer period and higher doses of ivermectin administration. As well as, pregnant females suffered fromabortion in some cases with fetal death. We concluded that ivermectin has adverse effect on male rabbits thatreceived either therapeutic or double therapeutic doses that varied from mild degenerative changes to completenecrosis of spermatogenic cells with complete absence of sperms. Meanwhile, female genital system wasseverely affected that showing severe degeneration and hemorrhage in uterus as well as atritic follicles anddegenerated ova in ovaries. So, caution must be considered for ivermectin administration especially duringbreeding season and for pregnant females.}, keywords = {Ivermectin,Pathology,Male rabbits,pregnant female rabbits}, url = {https://bvmj.journals.ekb.eg/article_31162.html}, eprint = {https://bvmj.journals.ekb.eg/article_31162_fece43fcb7398561c3fe9204335037fc.pdf} } @article { author = {Ibrahim, Hemmat and El Sabagh, Rasha and Abou El-Roos, Nahla and Mohebat, Abd El kareem}, title = {Mycotic quality of locally manufactured smoked herring fish}, journal = {Benha Veterinary Medical Journal}, volume = {32}, number = {1}, pages = {113-120}, year = {2017}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2017.31165}, abstract = {A grand total of 90 random samples of smoked herring fishes (30 Un packed –30 packed – 30 fillet) were collected fromdifferent markets at Monofia governorate. The samples were subjected to mycological examination for count, isolationand identification of mould and yeast. Also Molecular identification of aflatoxinogenic A. flavus was carried out. Theincidence of mould in the examined samples (unpacked, packed and fillet) were 100%, 83.3% and 66.7%, respectively.While the incidence of yeast in the examined samples (unpacked, packed and fillet) were 90%, 66.7% and 33.3%,respectively. The mean total mould counts /g was 1.9×105, 2.1×104, 2.5×103 for unpacked, packed and fillets, respectively.The mean total yeast counts/g was1.7×105, 2.1×104,1.3×102, respectively. The results indicated that un packed smokedherring fish were more contaminated with mould and yeast. Molecular identification of aflatoxinogenic A. Flavus waspositive for three sets of different virulence genes (omtA, ver-1, and nor-1) which involved in aflatoxin biosyntheticpathway.}, keywords = {Smoked herring,Mycotoxins,Aflatoxin biosynthesis,Aspergillus flavus}, url = {https://bvmj.journals.ekb.eg/article_31165.html}, eprint = {https://bvmj.journals.ekb.eg/article_31165_c3c06a42126f5132c3219980f92f7819.pdf} } @article { author = {Othman, Huda and Abdelsamei, Hamdi and Abdou, Adham and Ibrahim, Ekbal and Elbaba, Azza}, title = {Comparative studies of Listeria monocytogenes survival in bifidus and traditional yoghurt during production and storage}, journal = {Benha Veterinary Medical Journal}, volume = {32}, number = {1}, pages = {121-126}, year = {2017}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2017.31168}, abstract = {Traditional yoghurt (TY) (Streptococcus thermophiles+ Lactobacillus bulgaricus) and Bifidus yoghurt (BY) samples(Streptococcus thermophiles+ Lactobacillus bulgaricus+ Bifidobacterium bifidum). Traditional & Bifidus yoghurtsamples were inoculated with 106 cfu/g L. monocytogenes and stored for 21 days at 4±1oC. Samples were taken at zerotime (fresh samples) then after 3, 5, 7, 10, 14 and 21 days of storage for titratable acidity measurement and bacterial countof the culture starters and L. monocytogenes counts. The results revealed that the titratable acidity of TY & BY wasincreased during the 21 days of storage, while the population of L. monocytogenes became non-detectable level in 10thdays of storage in BY and still detected in TY till the day 14th of storage and became 2.20± 0.31 log10 cfu/g. The countof all starter cultures (S. thermophiles+ Lb. bulgaricus+ B. bifidum) in both yoghurts increased during the fermentationand cold storage and remained stable with values >6 log10 cfu/g throughout the storage period at 4±1°C.}, keywords = {probiotics,Bifidobacteria,L. monocytogenes,yoghurt,bifidus yoghurt}, url = {https://bvmj.journals.ekb.eg/article_31168.html}, eprint = {https://bvmj.journals.ekb.eg/article_31168_acbc4ef04d67c21fa70ae9e4c594dc30.pdf} } @article { author = {Othman, Huda and Elbaba, Azza and Abdelsamei, Hamdi and Abdou, Adham and Ibrahim, Ekbal}, title = {Evaluation the effect of Lactobacillus acidophilus probiotic culture over Staphylococcus aureus during the production and storage of acidophilus yoghurt}, journal = {Benha Veterinary Medical Journal}, volume = {32}, number = {1}, pages = {127-131}, year = {2017}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2017.31197}, abstract = {The survival of Staphylococcus aureus in the acidophilus yoghurt (AY) was evaluated during the production and coldstorage. 21 acidophilus yoghurt samples were prepared with traditional yoghurt culture (Streptococcus thermophiles &Lactobacillus bulgaricus) with Lactobacillus acidophilus and inoculated with 106 cfu/ml S. aureus and stored for 21 daysat 4oC. Samples were taken at zero time (fresh samples), 3, 5, 7, 10, 14 and 21 days for titratable acidity, culture starters’counts and S. aureus count. The results revealed that the titratable acidity% of AY was increased and reached 0.72, 0.78,0.83, 0.87, 0.89, 1.02 and 1.45% at zero time, 3, 5, 7, 10, 14, and 21 days of storage, respectively, while the population of S. aureus reached 6.10 and 3.70 log10cfu/g at zero time and 3rd day of storage, respectively and became non-detectable level in 5th days of storage. The count of all starter cultures (S. thermophiles+ L. bulgaricus+ L. acidophilus) in acidophilus yoghurt increased during the fermentation and cold storage, S. thermophiles became 7.15, 7.35, 8.20, 8.15, 7.45, 7.45 and 6.65 log10cfu/g while L. bulgaricus 7.25, 7.55, 8.25, 8.73, 7.76, 7.47 and 6.54 log10cfu/g and L. acidophilus became 7.38, 7.70, 8.25, 8.20, 7.50, 7,46 and 6.66 log10cfu/g at zero time, 3, 5, 7, 10, 14, and 21 days of storage, respectively. It concluded that S. aureus count decreased gradually during the production and cold storage and became non-detectable level on the 5th days of cold storage. The survival of all starter cultures in yoghurt sample remained stable with values > 6 log10 cfu/g throughout the storage period at 4±1°C.}, keywords = {probiotic,Lactobacillus acidophilus,S. aureus,yoghurt,acidophilus yoghurt}, url = {https://bvmj.journals.ekb.eg/article_31197.html}, eprint = {https://bvmj.journals.ekb.eg/article_31197_912b2397225a1d92979c86befdadac05.pdf} } @article { author = {Abd El-Tawab, Ashraf and Hofy, Fatma and Mohamed, Sahar and Amin, Sohila}, title = {Characterization of Methicillin Resistance Staphylococcus aureus isolated from chicken and human}, journal = {Benha Veterinary Medical Journal}, volume = {32}, number = {1}, pages = {132-137}, year = {2017}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2017.31198}, abstract = {Staphylococcus aureus causes a wide range of diseases in human beings, from minor skin infections to severe illnessessuch as septicemia, toxic shock, endocarditis, and pneumonia. Staphylococcus aureus is a major pathogen of increasingimportance due to the rise in antibiotic resistance. S. aureus was an important cause of disease in poultry it could beinvolved in a wide range of clinical conditions such as septicemia, bone and Joint infections, abscesses and dermatitis.Inthis study, 160 samples were collected , out of which 80 samples were from poultry and human patients (80 for each ).The incidence of infection with S.aureus in Bumble foot, Skin swabs in chicken and Diabetic foot swab , Skin sawb andNasal swab in human were, 10%, 66%, 30%, 40%, 67.5% respectively. Methicillin resistant S. aureus (MRSA) wereincidence in chicken and human 66.6% and 33.3% respectively. Subjected to PCR for detection of some antimicrobialresistance genes using intrinsic methicillin resistant gene (mec A), the IgG binding region of protein A( spa) gene were83.3% and 50 % respectively also beta-lactamase (blaZ) gene 100% and 100% respectively were detected in most MRSAisolates either from human or chicken samples}, keywords = {Methicillin resistant – S.aureus – human – chicken – mec A gene – blaZ gene –spa gene}, url = {https://bvmj.journals.ekb.eg/article_31198.html}, eprint = {https://bvmj.journals.ekb.eg/article_31198_3fc232b9e8fa9807b3bd62e8c24637f9.pdf} } @article { author = {Abukady, Khalid and Shaltout, Fahim and Amin, Reham and El-Shatter, Muhammad and Sabike, Islam}, title = {HACCP application during different processing stages of beef fillet with special references to corrective action}, journal = {Benha Veterinary Medical Journal}, volume = {32}, number = {1}, pages = {138-144}, year = {2017}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2017.31199}, abstract = {Here, in this study HACCP program was applied for the production of beef fillet in an Egyptian hotel and the microbialload before and after application was compared. The aerobic plate count monitored before and after application ofcorrective action during different successive production stages of beef fillet in tested hotel to examine the frequency ofcontamination. The mean values of Aerobic plate count before application of corrective actions were 20.47 ± 7.01, 71.33± 15.27, 680.0 ± 385.53, 528.67 ±255.39, 24.33 ± 8.09, and 24.33 ± 8.09 (CFU/gm) for the examined frozen, thawed,formed, salted, cooked and served meat, respectively. After application of corrective action, the aerobic plate countdeclined to be 13.80 ± 4.20, 48.00 ± 7.32, 118.67± 26.97, 92.67 ± 20.34, 7.13 ± 1.95, and <10 in the examined frozen,thawed, formed, salted, cooked and served meat, respectively. The reduction percentages for the above examined sampleswere 32.5%, 32.7, %82.5%, 82.4%, and 70.6% and 100%, respectively. It is noticed that meat meals can be contaminatedby several ways such as incorrect thawing, inadequate cleaning and sanitation for utensils or cross contamination aftercooking resulting in higher contamination with microorganisms. No food borne pathogens of public health hazardsdetected during this study. This results shows the significant importance of HACCP program for prevention and controlof pathogenic and spoilage microorganisms.}, keywords = {HACCP,aerobic plate count,beef fillet,Salmonella,E. coli}, url = {https://bvmj.journals.ekb.eg/article_31199.html}, eprint = {https://bvmj.journals.ekb.eg/article_31199_ccf58cfba2bb6ee5aa5062e135c2ba57.pdf} } @article { author = {AbdEL-Tawab, Ashraf and El-Hofy, Fatma and Abou El-Roos, Nahla and Abdullah, Hassnaa}, title = {Food hygiene Dept., Faculty of Veterinary Medicine, Benha University}, journal = {Benha Veterinary Medical Journal}, volume = {32}, number = {1}, pages = {145-152}, year = {2017}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2017.31201}, abstract = {The present study was performed on a total of 124 milk samples from small scale producers, farmers and markets at El-Menofyia Governorate. The prevalence of Streptococcus species in the examined samples was (65.3%), where S.agalctiae, S. dysgalactiae, S. uberis, S. pyogenes and S. pneumoniae were 28.2%, 11.3%,16.1%, 8.9% and 0.8%;respectively. The antibiogram for Streptococcus spp. revealed that vancomycin and erythromycin were the most properantibiotics with the highest efficiency against isolated Streptococcus spp., but they were resistant to cefatriaxone andchlormphenicol. Additionally, S. agalactiae and S. dysgalactiae were sensitive to penicillin, ofloxacin ;respctively.However, S. uberis was sensitive to amoxicillin and clindamycin. By using PCR, virulence gene hyalurinidase (hyl) wasdetected in 25% of S. agalactiae, while a surface expressed M-like protein (mig) gene was detected in 100% of S.dysgalactiae. Also plasminogen activator (pauA) gene was detected in 100% of S. uberis isolates}, keywords = {Streptococcus,Raw milk,virulence gene,PCR}, url = {https://bvmj.journals.ekb.eg/article_31201.html}, eprint = {https://bvmj.journals.ekb.eg/article_31201_8908403a9070c15ee297a4b48b98b59b.pdf} } @article { author = {ShafiK, Basant and Kh., El-Bayomi and S., Abo-Salem, and A., Darwish}, title = {Environmental Factors Affecting Some Productive and reproductive Traits in Egyptian Buffaloes}, journal = {Benha Veterinary Medical Journal}, volume = {32}, number = {1}, pages = {153-159}, year = {2017}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2017.31202}, abstract = {This study was conducted to evaluate the effect of some environmental factors on milk production in Egyptian buffaloesand the effect of parity and season of calving on calving interval and days open. There was a significant effect of parity(P ≤ 0.05) on 305day milk yield, while season of calving had a highly significant effect (P ≤ 0.01) on 305day milk yield.Animals with age at first service less than 21 months and age at first calving less than 35 months had higher milk yieldthan other animals (2489.5 and 2526.7 kg, respectively). With increasing days open, milk production increased so animalshad more than 160 days open produced 2757.8 kg milk. Milk yield in winter (2600.5 kg) was higher than in summerseason (2441.5 kg). Animals which conceived four or more services produce more milk yield (2529.8 kg). Season ofcalving had a significant effect (P ≤ 0.05) but parity had a highly significant effect (P ≤ 0.01) on calving interval and daysopen. There was direct relationship between milk yield with each of calving interval and days open. Animals that gavemore than 3000 kg showed the maximum calving interval and days open (14 months and 111.8 days, respectively)}, keywords = {calving interval,305day milk yield,Egyptian Buffaloes,Paritym Season of calving,Services}, url = {https://bvmj.journals.ekb.eg/article_31202.html}, eprint = {https://bvmj.journals.ekb.eg/article_31202_f2e7e909f5b9c81cc304b21fca3cec07.pdf} } @article { author = {ShafiK, Basant and Kh., El-Bayomi and S., Abo-Salem and A., Darwish and Ramadan, Sherif}, title = {Detection of SNPs in Exon10 Locus of FSHR Gene and Their Association with Some Infertility problems in Egyptian Buffaloes}, journal = {Benha Veterinary Medical Journal}, volume = {32}, number = {1}, pages = {160-169}, year = {2017}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2017.31203}, abstract = {Follicle-stimulating hormone (FSH) is a glycoprotein hormone. It helps in development and maturation of thefollicles in females through binding to FSH receptors which consists of 10 exons and 9 introns. The objectiveof this study was to estimate some genetic factors affecting milk production and fertility traits and to detectpolymorphisms in exon 10 of FSHR gene and determine the associations between these polymorphisms andinfertility in Egyptian buffalo. Heritability estimates were (0.19, 0.18, 0.07, 0.10, 0.21 and 0.19) for calvinginterval, days open, dry period, days in milk, total milk yield and 305day milk yield (305 DMY), respectively.Dry period showed high positive phenotypic and genetic correlation with day's open and calving interval. Thebreeding value for 305 DMY ranged from -480 to 380, from -370 to 330 and between -230 to 170 kg for cow,sire and dam, respectively. There was one non synonymous SNP (A93G) at 93bp in exon 10 of FSHR gene(with 230bp size) and there was two different pattern of single strand conformation polymorphism (SSCP) butthere was no any SNP for FSHR gene (with 306bp size) in all examined Egyptian buffaloes as shown by oneSSCP pattern and nucleotide sequencing.}, keywords = {correlation. Egyptian buffaloes. FSHR. heritability. Polymorphism. SNP. SSCP}, url = {https://bvmj.journals.ekb.eg/article_31203.html}, eprint = {https://bvmj.journals.ekb.eg/article_31203_03553a06d15969ae986e92f078371a60.pdf} } @article { author = {Salem, Amani and A., Abd El Raheem and M., Zakaria}, title = {Environmental Contamination with Methicillin Resistant Staphylococci in Food animal carcasses}, journal = {Benha Veterinary Medical Journal}, volume = {32}, number = {1}, pages = {170-176}, year = {2017}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2017.31204}, abstract = {One hundred random samples of cattle and sheep meat, equipment and workers hands swabs (25 samples of each) werecollected from different abattoirs in Kalyobia governorates. The obtained results indicated that the mean values of APC,and S. aureus counts in the examined samples were 1.5 x 104 ± 0.9 x 104, 1.77 x 103 ± 1 x 103CFU /g for cattle meat, 1 x104 ± 0.65 x 104, 1.57 x 105 ± 0.83 x 105 CFU /g for sheep meat, 7.4 x 103 ± 0.29 x 104, 1.1 x 103 ± 0.33 x 103 CFU /g forequipment swab and 5.77 x 103 ± 0.3 x 104, 7.4 x 103 ± 0.49 x 104CFU /g, for workers hands swab, respectively. Theincidence of S. aureus was 52%, 48%, 56% and 64% in the examined samples of cattle and sheep meat, equipments andworkers hands swabs, respectively. On the other hand, Methicillin Resistant S. aureus (MRSA) was isolated from 32%,24%, 40% and 44% of the examined samples of cattle and sheep meat, equipments and workers hands swab respectively.The significance of the isolated bacteria in the examined animal carcasses at abattoir level and possible sources ofcontamination as well as some recommendations to improve the quality of these carcasses were discussed}, keywords = {meat,abattoir,S. aureus,MRSA,carcass}, url = {https://bvmj.journals.ekb.eg/article_31204.html}, eprint = {https://bvmj.journals.ekb.eg/article_31204_684d8975a3a3fe003acee5f5e5a95d79.pdf} } @article { author = {Salem, Amani and A., Abd El -Raheem and M., Zakaria}, title = {Efficiency of some essential oils in Control of Methicillin Resistant Staphylococcus Aureus (MRSA) in Minced Beef}, journal = {Benha Veterinary Medical Journal}, volume = {32}, number = {1}, pages = {177-183}, year = {2017}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2017.31205}, abstract = {In this study, the antibacterial effect of Cinnamon, Thyme and Lemongrass essential oils against Methicillin ResistantStaphylococcus aureus (MRSA) artificially inoculated into refrigerated minced beef was investigated. Sensory analysisindicated significant advantages in using these oils in refrigerated minced beef. Also, results indicated that the MRSAcounts decreased as the concentration of the oil increases since the concentration (1.5%) gives the best effectiveness. Inaddition, a highly significant differences (P<0.05) as a result of oil treatments were noticed. The antibacterial activitiesof the added essential oils followed the order, Cinnamon > Thyme > Lemongrass oil. The shelf life of the treated sampleswas extended than control ones. In conclusion, these oils especially cinnamon one can be useful for commercialapplication as potential food preservatives and anti- MRSA agents in food}, keywords = {MRSA,Essential oils,minced beef,Antimicrobial}, url = {https://bvmj.journals.ekb.eg/article_31205.html}, eprint = {https://bvmj.journals.ekb.eg/article_31205_809b0abccb5c5f65bf473af8ba00d052.pdf} } @article { author = {Abd El- Tawab, Ashraf and El- Hofy, Fatma and Nasef, Soad and Ibrahim, Ola}, title = {Prevalence of eaeA and qacEΔ1 genes in Escherichia coli isolated from omphalitis in baby chicks}, journal = {Benha Veterinary Medical Journal}, volume = {32}, number = {1}, pages = {184-192}, year = {2017}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2017.31206}, abstract = {A total of two hundred diseased Saso chicks with omphalitis were examined (1400 samples) for the isolation of E. colifrom different organs (liver, caecum, spleen, heart, lung, yolk sac and cloacal swab). Results showed that 64 cases werepositive with an incidence of 32%. Fifty isolates (25%) of E. coli were recovered from chicks could be sero-grouped in19 O groups with the most predominant serotype was O91 20 % (10 out of 50 isolates), O26 10% (5 strains out of 50) O788% (4 strains out of 50), and 7 strains untypable E. coli 14%. Among E. Coli O groups were found to be resistant toAmoxicillin 93.02% followed by Tetracycline 74.42%, Enrofloxacin 46.51%, Erythromycin 30.32%, Ciprofloxacin27.91%, Norofloxacin and Streptomycin 20.93% and Gentamycin 6.98%. While they were found to be sensitive forGentamycin, Streptomycin, Ciprofloxacin, Tetracycline, Erythromycin, Norofloxacin, Enrofloxacin, and Amoxicillin asthe following: 88.37%, 46. 51%, 34.89%, 23.26%, 18.60%, 9.30%, 9.30% and 6.98%, respectively. The incidence rateof eaeA gene of E. coli was 15.79%. Quaternnary ammonium compound (qacEΔ1) gene also was detected in E. coli withincidence rate 63.16%.}, keywords = {Omphalitis,E. coli,virulence gene,Antibiotic resistance,Disinfectant resistant gene}, url = {https://bvmj.journals.ekb.eg/article_31206.html}, eprint = {https://bvmj.journals.ekb.eg/article_31206_f2aeaa13f19f20e0070b433695be00a5.pdf} } @article { author = {Youssuf, Hadeer and Abdel Gwad, Eman and El-Asely, Amel and Elabd, Hiam and Shaheen, AA and Abbass, Amany}, title = {Isolation of Aphanomyces invadans Associated with Skin Lesions in African Catfish" Clarias gariepinus"}, journal = {Benha Veterinary Medical Journal}, volume = {32}, number = {1}, pages = {193-197}, year = {2017}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2017.31207}, abstract = {The present study aimed to isolate and identify of Aphanomyces invadans from naturally infected African Catfish" Clariasgariepinus". A total number of 105 cultured Clarias gariepinus collected from private fish farms in Kafr Al-Sheikh &Behera Governorates. Result revealed that the prevalence of infection among the examined fish was 8.6 % and the diseasemostly recorded during winter and spring. The infected fish developed characteristic ulcerative lesions and fin rot.Fungus-like oomycetes Aphanomyces invadans grow on glucose peptone yeast (GPY) agar as opaque colonies withuneven white transparent velvets surface at room temperature. Microscopically, lactophenol cotton blue stained fungalgrowth appeared as non-septated thin long branched hyphae with tapered end containing cytoplasmic organelles. Onsporulating media, the rectangular shaped spores appeared inside the hyphae connected together by thin filament. Inconclusions, EUS is an invasive disease of Clarias gariepinus and care should be taken with low temperature in managingfish pond.}, keywords = {Aphanomyces invadans,Clarias gariepinus,identification,prevalence,sporulation}, url = {https://bvmj.journals.ekb.eg/article_31207.html}, eprint = {https://bvmj.journals.ekb.eg/article_31207_3dc3eb8e8130befe3663c3bf390e29c5.pdf} } @article { author = {Abd El-Tawab, Ashraf and Abdallah, Mohamed and Yusuf, Hend}, title = {Incidence and antibiogram of Clostridium perfringens isolated from herbs and spices widely distributed in the Egyptian market}, journal = {Benha Veterinary Medical Journal}, volume = {32}, number = {1}, pages = {198-206}, year = {2017}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2017.31208}, abstract = {The present study was designed for isolation and identification of Clostridium perfringens (C. perfringens) from herbsand spices that are widely distributed in the Egyptian markets; and determination of the drug of choice for its control basedon the Antimicrobial Susceptibility Test (AST). A total of 392 samples of herbs and spices were randomly andanonymously selected from the enormous number of samples obtained from the Egyptian markets. An overall occurrenceof C. perfringens was 8.42% (33 out of 392). Separately, occurrence was higher in herbs than spices being 10.5 and 6.25%,respectively. C. perfringens isolates were identified and confirmed with macroscopical, microscopical and biochemicalanalysis. AST results revealed that isolates of C. perfringens from the tested herbs and spices samples were found mostsusceptible to ampicillin-sulbactam and Penicillin-G with inhibition zones of 19.4 ± 0.98 and 16.6 ± 1.16 mm, respectively.On the other hand, the isolates were found nearly resistant to clindamycin and vancomycin with inhibition zones of 6.28± 0.63 and 8.78 ± 0.41 mm, respectively. Meanwhile, the isolates were intermediary susceptible to trimethoprimsulphamethoxazolecombination and chloramphenicol. Data of the present study indicate occurrence of C. perfringens inherbs and spices with a considerable rate and the antimicrobials of choice for its control are penicillins and ceftazidime.higher sanitation procedures in herbs and spices industry before its distribution to the Egyptian markets have beenrecommended}, keywords = {C. perfringens,Incidence,prevalence,herbs,Spices,AST}, url = {https://bvmj.journals.ekb.eg/article_31208.html}, eprint = {https://bvmj.journals.ekb.eg/article_31208_4bc9e8ef0333be661d56e01a9f10a727.pdf} }