@article { author = {Amer, Shimaa}, title = {Effect of Spirulina platensis as feed supplement on growth performance, immune response and antioxidant status of mono-sex Nile Tilapia (Oreochromis niloticus)}, journal = {Benha Veterinary Medical Journal}, volume = {30}, number = {1}, pages = {1-10}, year = {2016}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2016.31332}, abstract = {To evaluate the effect of Spirulina platensis inclusion in diets for Nile tilapia fingerlings, a completelyrandomized experimental design was developed with 4 treatments and three replicates. Diets with 4inclusion levels (control diet (Sp0), 0.5% (Sp0.5), 1% (Sp1) and 1.5% (Sp1.5)) were prepared. 180 Niletilapia fingerlings with average initial weight of 8.46±0.17 g were assigned to twelve experimental tanks.The experiment lasted for ten weeks. At the end of the experiment; growth performance, the whole bodycomposition was determined and serum samples for lysozyme, IgG, IgM values and catalase activitywere collected and antioxidant status of Nile tilapia muscle were determined. The results showed thatgroup supplemented with 1% S. Platensis have higher body weight and lower FCR than the controlgroup and other groups. S. platensis inclusion increased activity of glutathione reductase, and reduceMDA formation. Levels of lysozyme, IgG and catalase activity were higher in S. platensis supplementedgroups but don’t have significant effect on IgM level. In conclusion, Spirulina platensis supplementationincreased antioxidant protective capacities. It also affected some innate and humoral immunityparameters as well as don’t have negative effect on fish growth}, keywords = {Nile tilapia,Spirulina platensis,Growth performance,antioxidant status,innate and humoral immunity}, url = {https://bvmj.journals.ekb.eg/article_31332.html}, eprint = {https://bvmj.journals.ekb.eg/article_31332_ab3bb0a7b293335d8b5afe3e2a4f555d.pdf} } @article { author = {Abd El-Tawab, Ashraf and El Hofy, Fatma and Ammar, Ahmad and Sleim, Mokhtar and Salem, Heba}, title = {Prevalence and antimicrobial susceptibility pattern of Staphylococcus aureus isolated from dairy cattle's subclinical mastitis in EL-Sharkia Governorate}, journal = {Benha Veterinary Medical Journal}, volume = {30}, number = {1}, pages = {11-19}, year = {2016}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2016.31333}, abstract = {Subclinical mastitis is a disease of major economic importance to dairy industry causing reduced milkquality and loss in its production. Therefore, the present study was carried out for isolation andidentification the main pathogen responsible for this disease and detection the drug of choice. A total of475 milk samples of lactating dairy cows were collected from different localities in El-SharkiaGovernorate, and subjected to physical, chemical tests (California mastitis test (CMT) as well as Somaticcell count (SCC), bacteriological examination and antimicrobial susceptibility pattern. The prevalenceof subclinical mastitis at cow level was 21% (100/475). Accurately 80 Staphylococcal isolates wereidentified with prevalence of 61% S. aureus through beta hemolysis and coagulase positive. Theobtained S. aureus isolates were highly sensitive to vancomycin, gentamicin, ciprofloxacin andchloramphenicol with percentages of 93.4%, 83.6%, 82% and 80.3%, respectively. Meanwhile, highfrequency of resistance was observed to oxacillin, erythromycin and tetracycline, with percentages of59%, 55.7%, and 47.5%, respectively. Therefore, the main choice drugs for subclinical mastitis werevancomycin and gentamicin}, keywords = {Subclinical mastitis,California mastitis test,Antibiotic Susceptibility}, url = {https://bvmj.journals.ekb.eg/article_31333.html}, eprint = {https://bvmj.journals.ekb.eg/article_31333_76757d9f0008c348e1370d0e427fd988.pdf} } @article { author = {Abd El Tawab, Ashraf and El-Hofy, Fatma and Ammar, Ahmed and Saleh, Osama and Tolba, Heba}, title = {Effect of prebiotic on the immune status of Oreochromis niloticus}, journal = {Benha Veterinary Medical Journal}, volume = {30}, number = {1}, pages = {20-28}, year = {2016}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2016.31335}, abstract = {A total number of 180 Nile-tilapia (Oreochromis niloticus) were divided into four equalgroups; the first one served as control fed on basal non-treated diet. The second group fed ondiet supplemented with 0.5 ml prebiotic βpolo /kg basal fish diet, the third group on dietsupplemented with 1 ml prebiotic βpolo/kg basal fish diet, and the fourth group fed on dietsupplemented with 1.5 ml prebiotic β polo/kg basal fish diet for 40 days. The result elevatedthat, the growth performance was significantly higher in all treatments than in the control groupand the highest increased was in the group received 1.5 ml prebiotic βpolo /kg basal fish diet.The hematocrit (HC) values, nitroblue-tetrazolium (NBT) and lysozyme activities wereincreased significantly in all treated groups than the control and the highest increased was inthe group received 1.5 ml prebiotic βpolo /kg basal fish diet. The percent level of protectionamong the three treated groups after challenge infection using Pseudomonas flourescence (Ps.flourescence) (0.5 ml of culture suspension of pathogen containing 107 bacteria ml-1) washigher than control and the highest record was in the group of received 1.5 ml prebiotic βpolo/kg basal fish diet}, keywords = {prebiotic,Immune Response,Ps. flourescence,Nile tilapia}, url = {https://bvmj.journals.ekb.eg/article_31335.html}, eprint = {https://bvmj.journals.ekb.eg/article_31335_61ccf8813ffc63cb619768fd306df412.pdf} } @article { author = {F.Y., El-Regily and M., El-Hindawy and M., Sadek and A., Abd El-Maksoud}, title = {Biochemical alterations of inflammatory markers in experimentally induced diabetes in rats}, journal = {Benha Veterinary Medical Journal}, volume = {30}, number = {1}, pages = {29-38}, year = {2016}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2016.31341}, abstract = {This study was evaluated the protecting effect of rutin on the glycemic condition, lipid profile, instreptozotocin (STZ)-induced experimental diabetic in rats. With respect to an orally administrated rutinon serum glucose, the result is showing a significant decrease in glucose concentration when comparedto diabetic group, While, a significant increase in insulin concentration when compared to diabetic.Moreover, the results revealed that, an administration of streptozotocin (STZ) showing significantincrease in triacylglycerol, total cholesterol, LDL-c and VLDL-C concentration when compared withcontrol group, while, showing significant decrease in HDL-C concentration and serum AST and ALTactivity when compared to the control group. The data revealed also significant increase in serum LMDAconcentration. While, significant decrease in serum calcium concentration when compared to thecontrol group}, keywords = {streptozotocin,Rutin,glucose,Insulin,L-MDA,calcium}, url = {https://bvmj.journals.ekb.eg/article_31341.html}, eprint = {https://bvmj.journals.ekb.eg/article_31341_2ebdf4744fcadfcb7ec2b646367a5ab7.pdf} } @article { author = {Mahfouz, Mohamed and Zyan, Kamel and Abdel-Hamid, Hossam}, title = {Biochemical effect of β-hydroxy-β-methylbutyrate calcium and creatine supplementations on some blood parameters, pro-inflammatory cytokines and growth performance of broiler chicks}, journal = {Benha Veterinary Medical Journal}, volume = {30}, number = {1}, pages = {39-50}, year = {2016}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2016.31342}, abstract = {One hundred and sixty-five one-day old Cobb broiler chicks were allocated into 3 groups to investigate theeffect of dietary 0.1% β-hydroxy- β-methylbutyrate calcium (HMB) and 0.1% creatine on some bloodparameters, pro-inflammatory cytokines and growth performance parameters in terms of total feed intake(TFI), total body weight gain (TBWG), total body weight and feed conversion ratio (FCR) during certaintime intervals (21st day and 42nd day) versus control group (0 treatment). Some biochemical changes wererecorded at the 42nd day among creatine supplemented group in the form of significantly high results of bothtriglycerides and T3 in group 3 (creatine) than that of the control group on the same day at P value > 0.05.HMB supplemented group showed significantly lower results of ALT (mean) than that of control group at(P value > 0.05) on the 21st day which became insignificant at the 42nd day. Dietary 0.1% HMB-Ca recordedbetter growth performance parameters in terms of TFI (4.527), TBWG (2.515), TBW (2.555) and TFCR(1.8) if compared to creatine supplemented group of (4.664, 2.508, 2.549 and 1.86) respectively and thecontrol group of TFI equaled to 4.7432, TBWG equaled to 2.156, TBW equaled to 2.1987 and TFCRequaled to 2.2 at the end of the experiment. HMB and creatine supplementations had no effect on otherblood parameters, viz. AST, urea, creatinine, total protein, FFA, T4, T3/T4 ratio, TSH, uric acid, cholesterol,HDL and LDL, and pro-inflammatory cytokines namely IL-6, IL-2 and TNF-α.}, keywords = {HMB-Ca,Creatine,broiler chicks,TFI,FCR,TBWG,TBW}, url = {https://bvmj.journals.ekb.eg/article_31342.html}, eprint = {https://bvmj.journals.ekb.eg/article_31342_9f373e9facd1e5ed9fcf1b07828f965e.pdf} } @article { author = {Khalafalla, Fathy and Ali, Fatma and Hassan, Abdel-Rahim and El-Feky, Kareem}, title = {Monitoring the bacterial contamination during different stages of beef carcass preparation at Beni-Suef abattoir, Egypt}, journal = {Benha Veterinary Medical Journal}, volume = {30}, number = {1}, pages = {51-58}, year = {2016}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2016.31345}, abstract = {In an attempt to monitor the levels of bacterial contamination during different stages of beef carcasspreparation at Beni-Suef abattoir, Egypt, 20 beef carcasses were examined. Each carcass wasrepresented by swabs from the abdomen, neck, shoulder, thigh and thorax, collected after dehiding, afterevisceration and after complete preparation. Each swab was examined for the total mesophilic,psychrophilic and staphylococcal counts, MPN of coliforms, fecal coliforms and E. coli. The mesophiliccounts in the examined swabs of abdomen, neck, shoulder, thigh and thorax after dehiding were 3×106,3×106, 5×106, 7×105, 3×106, while after evisceration, they changed to 2×107, 5×106, 4×106, 5×105, 2×106CFU/cm2, respectively. Moreover, they accounted for 5×105, 5×106, 7×106, 2×105, 5×106 CFU/cm2 afterpreparation, respectively. The staphylococcal counts in the examined swabs were 7×102, 7×102, 8×102,7×102, 8×102 after dehiding, 6×102, 9×102, 6×102, 6×102, 4×102 CFU/cm2 after evisceration,respectively. While, they were 4×102, 9×102, 10×102, 6×102, 3×102 CFU/cm2 after preparation,respectively. As regard to coliforms (MPN) in the examined swabs after dehiding, they were 2×10,4×102, 2×102, 4×102, 2×102, while 9, 3×102, 1×102, 3×102, 1×102 after evisceration, and <3, 3×102,8×10, 2×10, 3×10 m.os./cm2 after preparation, respectively. The sources of contamination with thedetermined bacteria were discussed throughout the study}, keywords = {abattoir,Bacterial contamination,APC,beef,coliforms MPN}, url = {https://bvmj.journals.ekb.eg/article_31345.html}, eprint = {https://bvmj.journals.ekb.eg/article_31345_44ef810de2c2afd751a951629fd285ac.pdf} } @article { author = {A., Nasr and M., Marwah and M., Abdel Rahman and H., Shafeek}, title = {Comparison of modified decontamination methods with culture systems for primary isolation of Mycobacterium bovis from bovine tissues}, journal = {Benha Veterinary Medical Journal}, volume = {30}, number = {1}, pages = {59-67}, year = {2016}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2016.31346}, abstract = {A definitive diagnosis of M. bovis infection in animals depends on the isolation of the organism, as theoptimum condition for the primary isolation of M. bovis infection has not been determined. In this studya total number of 2550 cattle from different governorates in Egypt were tested by Single IntradermalCervical (SID) tuberculin skin test. Positive reacted cattle (no= 42, 1.6%) were slaughtered andsuspected lesions were collected, these samples were decontaminated by using three different methods,which were 0.75 % Hexadecyl Pyridinium Chloride (HPC), 5% oxalic acid and 6 % sulphuric acid.Isolation of mycobacteria from 42 samples by using these decontaminated methods were of 34 (80.9%), 30 (71.4 %) and 28 (66.6 %), respectively. So it could be concluded that using HPC method asdecontaminated agent revealed high rate of isolation of mycobacteria with less contamination (7.1 %)than the other two methods}, keywords = {Mycobacterium bovis – HPC- decontamination – oxalic acid – sulphuric acid}, url = {https://bvmj.journals.ekb.eg/article_31346.html}, eprint = {https://bvmj.journals.ekb.eg/article_31346_a2e414edfd4e589301f1ecc0b4eacad6.pdf} } @article { author = {Abd El Tawab, Ashraf and El-Hofy, Fatma and Ammar, Ahmed and Abdel Hakeem, Mokhtar and Abdel Galil, Noha}, title = {Preliminary studies on E. Coli implicated in avian Colibacillosis with reference to their antibiotic resistance profiles}, journal = {Benha Veterinary Medical Journal}, volume = {30}, number = {1}, pages = {68-77}, year = {2016}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2016.31348}, abstract = {Colibacillosis constituted as one of the most important disease condition affecting poultry withsignificant implications on production. Herein, examination of 451 organ samples including trachea,lung, heart blood, liver and spleen collected from freshly slaughtered chickens using standard methodsfor isolation and identification of E. coli showed that 236 samples were positive for E. coli with anincidence rate (52%). Twenty-five E. coli strains were serogrouped and 6 different serogroups weresuccessfully identified, the most prevalent serogroup was O78 (20%), followed by O157 and O27 withincidence rate (16%) for each, O168 and O125 (12%) each. and finally O115 (8%), in addition to 4untypable strains. The antibiotic susceptibility of 60 isolates against ten antimicrobials was performedby disc diffusion method and the results revealed that gentamicin and colistin were the most effectiveantibiotics on the isolates. On the other hand, all isolates exhibited absolute resistance to erythromycinand rifampicin, followed by high level of resistance ranging from (75-95%) for ciprofloxacin,sulfamethoxazole–trimethoprim, cefoxtin, chloramphenicol, doxycycline and amoxicillin- clavulanicacid. All isolates exhibited multidrug resistance phenotypes}, keywords = {Escherichia coli,Biochemical identification,Serogrouping,Antibiotic resistance}, url = {https://bvmj.journals.ekb.eg/article_31348.html}, eprint = {https://bvmj.journals.ekb.eg/article_31348_b9f7da65fdad13a57ecc394606d32c7f.pdf} } @article { author = {El-Sayed, Effat and Zaki, Eman and El-Kholy, A. and Salama, Sleem}, title = {Efficacy of the E. Coli whole cell lysates for improving immunogenic quality of the local entero-3 vaccine}, journal = {Benha Veterinary Medical Journal}, volume = {30}, number = {1}, pages = {78-85}, year = {2016}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2016.31349}, abstract = {Bacterial cell lysates of E. coli strains (K99 and O 157) were prepared and used as vaccinal antigensadded to locally prepared Entero-3 inactivated vaccine components aiming to improve the immuneresponse of calves against E. coli. Three groups of apparently healthy crossbred, three calves per group,were vaccinated with two doses (4ml I/M /dose), three weeks apart as follow: the first group (G.Ι) wasvaccinated by local inactivated Entero-3 vaccine (Rota, Corona and E. coli K99). The second group(G.ΙΙ) was vaccinated by vaccine containing Rota, Corona and cell lysate of E. coli K99 & E. coli O157.The third one (G.ΙΙΙ) was vaccinated by vaccine containing Entero-3 vaccine component and cell lysateof E-coli K99 & E-coli O157, and the fourth group kept unvaccinated served as control. The antibodyresponse in sera of vaccinated calves measured by serum neutralization test (SNT) and ELISA, whichrevealed that all types of the prepared vaccines induced a protective antibody titres. Entero-3 vaccinecontaining cell lysate of E-coli strains induced higher response than the other two vaccines. Inconclusion, adding of E. coli strains lysates as a vaccinal antigen to the locally prepared Entero-3vaccine, provided a formulation of Entero -3 vaccine that conferred a good immune response of calvesagainst collibacellosis}, keywords = {Entero-3,E. coli,vaccine,lysate,Collibacellosis,calves}, url = {https://bvmj.journals.ekb.eg/article_31349.html}, eprint = {https://bvmj.journals.ekb.eg/article_31349_b8b445522ea25cc02d4de0f69a15a04b.pdf} } @article { author = {A., Nagwa and S., Elmadawy and M., Ghanem and S., ELdiarby}, title = {Prevalence and molecular analysis of anaplama and piroplasmid species infecting buffaloes in qualyubia governorate, Egypt}, journal = {Benha Veterinary Medical Journal}, volume = {30}, number = {1}, pages = {86-96}, year = {2016}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2016.31350}, abstract = {This study aimed to investigate the prevalence of Anapalsma and piroplasmid species infecting buffaloeswith regard to season, age and sex. To achieve this aim, blood samples from a total of 530 apparentlyhealthy and clinically suspected buffaloes in Qualyubia governorate, Egypt were collected throughoutthe period from June 2013 to May 2014. The blood samples were smeared, stained with Giemsa andexamined by conventional microscope. Moreover, PCR assay was applied to amplify DNA of Babesiaand Theileria sp. in 100 microscopically negative buffaloes' blood samples. The microscopicinvestigation identified the infection in 37.92% of the examined buffaloes. Anaplasma marginale wasthe most prevalent species (30.94%) followed by Babesia sp. (26.60%) and Theileria annulata (2.08%).The most frequent clinical signs observed during the course of disease, in addition to the effect ofseasons, age and sex on the prevalence of infection were presented in the study. PCR assay proved tobe the most reliable method for the diagnosis of piroplasmid infections as compared to light microscopy,where it was able to detect Babesia and Theileria sp. DNA in 25% and 12% with 5% mixed in themicroscopically negative blood samples of buffaloes respectively. The high prevalence of infection inbuffaloes highlights the need for establishment of a consolidated control program to overcome theeconomic losses caused by these parasites}, keywords = {Buffalo,Piroplasmosis,prevalence,PCR}, url = {https://bvmj.journals.ekb.eg/article_31350.html}, eprint = {https://bvmj.journals.ekb.eg/article_31350_31d00d2f025aa3725e2282a1ea9527c9.pdf} } @article { author = {Mohammed, Hamdi and Abdou, Adham and Eid, Amal and Zakaria, Asem}, title = {Rapid tests for detection of enrofloxacin residues in liquid milk}, journal = {Benha Veterinary Medical Journal}, volume = {30}, number = {1}, pages = {97-103}, year = {2016}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2016.31351}, abstract = {A total of 234 randomly selected milk samples from different sources (110, 50, 34 and 40 samples) fromeach of (market milk, collecting centers, bulk tank and UHT milk samples; respectively) were analyzedfor detection the quinolones residues by rapid quinolones detection strip test. Results revealed that17.3%, 14% and 20.6% of milk samples were positive for the quinolones residues for market milk,collecting centers and bulk tank milk samples; respectively, while not detected in UHT milk samples.The positive milk samples were subjected to HPLC analysis for quantitative detection of enrofloxacinresidues, which were not detected in market milk and collecting centers milk samples while detected inone sample from bulk tank milk samples. The mean value of 2.94 ppb that not exceeded the maximumresidual limit set by different international standards. Public health importance of enrofloxacin residueswas discussed}, keywords = {Rapid tests,Enrofloxacin,Liquid Milk}, url = {https://bvmj.journals.ekb.eg/article_31351.html}, eprint = {https://bvmj.journals.ekb.eg/article_31351_eca0ff0ab430f03ca458deabf97399fb.pdf} } @article { author = {Hassanien, Fatin and Nada, Shiamaa and Abd-Elsattar, Alaa}, title = {Incidence of E. coli in some meat products}, journal = {Benha Veterinary Medical Journal}, volume = {30}, number = {1}, pages = {104-108}, year = {2016}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2016.31353}, abstract = {A grand total of 100 random samples of meat products represented by beef burger and kofta (50 of each)100g were collected from different shops and hyper supermarkets in different districts in Menofiagovernorate. The incidence of E. coli in the examined samples of meat products were 28% and 36 %forbeef burger and kofta, respectively. Moreover, the isolated serotypes of E. coli from the examinedsamples of meat products were O26: H11, O86, O55: H7, O104: H4, O111: H4, O114: H21, O119: H4, O124, O125:H21, O167: H21 and O128: H2 with various percentages, Also, application of sensitivity test on all E. colistrains indicated that most of them were multiple antimicrobial resistance (MAR) where the MAR indexequal 0.515. The public health significance of the isolated organisms from meat products was discussedas well as some recommendations to ensure safety and quality of meat products reach to consumers}, keywords = {E. coli,meat products,Multiple antimicrobial Resistances}, url = {https://bvmj.journals.ekb.eg/article_31353.html}, eprint = {https://bvmj.journals.ekb.eg/article_31353_8ac30a843b24d669e9886c1ff4f8473c.pdf} } @article { author = {Hassanien, Fatin and El-Sabagh, Rasha and Nassief, Marionet and Refat, Mohammed}, title = {Bacterial and Chemical quality of Frozen Chicken Meat Received at Governmental Hospital modern}, journal = {Benha Veterinary Medical Journal}, volume = {30}, number = {1}, pages = {109-117}, year = {2016}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2016.31355}, abstract = {This study was conducted to evaluate the quality of frozen chicken meat received at governmentalhospital in kalyobia governorate, Egypt. A total of 100 samples were taken from chicken breast andthigh (50 of each), randomly for bacteriological and chemical examination. The average of APC,coliform and psychrotrophes counts were 3.78×106±0.93±106/g, 2.07×103±0.60×103/g and5.71×106±1.44×106/g for breast and 4.38×106±0.59×106/g, 2.61×103±0.60×103/g and4.59×106±1.26×106 /g for thigh, respectively. Chemical keeping quality tests represented by pH, TVB/N(mg/100g) and TBA (mg/kg) indicated that the mean values were 5.84±0.10, 18.99±0.59 and 0.68±0.01for chicken breast and 5.91±0.11, 19.28±0.60 and 0.73±0.02 for chicken thigh, respectively. Therecommended points were discussed}, keywords = {Frozen chicken meat,APC,Coliform,Psychrotrophes,pH,TVB/N and TBA}, url = {https://bvmj.journals.ekb.eg/article_31355.html}, eprint = {https://bvmj.journals.ekb.eg/article_31355_70c9a259cf290208c27eb66ee0542d41.pdf} } @article { author = {Abdel-Tawab, Ashraf and El-Hofy, Fatma and Maarouf, Ahmed and Abbas, Sameh}, title = {Bacteriological and immunological studies on some bacterial pathogens incriminated in bovine Mastitis}, journal = {Benha Veterinary Medical Journal}, volume = {30}, number = {1}, pages = {118-123}, year = {2016}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2016.31356}, abstract = {In this study a total of 1200 quarter milk samples were selected from 300 cows from different localitiesat El-Kaluobia governorate. The bacteriological examination revealed that a total of 125 isolates fromclinical and subclinical mastitic milk samples of which 57 S.aureus isolates were isolated from bothmastitic milk .with incidence of 45.6% of total isolates, 28 E.coli with 22.4%, 13 S.agalactia with 10.4%,9 S. dysgalactia with 7.2%, 7 isolates of both S.uberis & coagulase negative staph (5.6%), 3 Klebsiellasp with 2.4% and 1 Pseudomonas sp with 0.8%. Mean titer of anti S aureus specific IgA was higher inmilk of subclinical mastitic cases (1:845) than clinical one(1:290), while mean titer in milk of clinicaland subclinical mastitic cases much higher than in serum of clinical(1:54)and subclinical (1:41), whilemean titer of anti S. aureus specific IgM and IgG was much higher in serum of clinical(1:1382 , 1:868for IgM&1:1267 , 1:634 for IgG ) than milk of clinical and subclinical mastitic cases (1:111 , 1: 53 forIgM& 1: 302 , 1: 206 for IgG). Results of sensitivity tests of S.aureus against different antibiotic discsrevealed that 93%,91.2%,87.2% and 63.2%, were sensitive to Amoxicillin /Clavulanic acid,Gentamycin, Cloxacillin, Sulphamethoxazole /Trimethoprim while87.7%,86.0%,84.2% and 82.5%ofisolates were resistant to Nalidixic acid, Streptomycin, Oxytetracycline and Penicillin-G.}, keywords = {mastitis,cattle,immunoglobulins,Antibacterial}, url = {https://bvmj.journals.ekb.eg/article_31356.html}, eprint = {https://bvmj.journals.ekb.eg/article_31356_6da663f40a8b4b51efdd0fd00687239f.pdf} } @article { author = {abou Zaid, Omaima and El- Senousy, Yakout and Badawy, Abdel Fattah and Rashad, Ahmed}, title = {Biochemical Studies on Evaluation of New Composite against Hepatocellular carcinoma-induced in Rats: Recent Therapeutic Approaches}, journal = {Benha Veterinary Medical Journal}, volume = {30}, number = {1}, pages = {124-136}, year = {2016}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2016.31357}, abstract = {The hepatoprotective effect of some natural and synthetic compounds against chemically inducedhepatocellular carcinoma in rats was evaluated. One hundred male albino rats were divided equally intofive groups. Normal control group, carcinogenic [Ferric nitrilotriacetate (Fe-NTA: 9 mg Fe/kg b.wt. i.p.)and chloroform (150 mg/ kg b.wt. orally)] - induced group, curcumin group (400 mg/kg. b.wt. orally),tetrachlorocuprate-lysine (25 mg/kg. b. wt. s.c.) and ascorbate (500 mg/kg. b. wt. orally) group and amixture group (composed of curcumin, tetrachlorocuprate -lysine and ascorbate). Blood samples andliver tissue specimens were collected at the end of experiment (4 months) for determination of thefollowing parameters: Tumor necrosis factor-α (TNF-α), interleukin-1 (IL-1), catalase andmyeloperoxidase (MPO) in liver tissues, in addition to serum malondialdehyde (MDA) and reducedglutathione (GSH). Moreover, histopathological examination of liver tissues was done for resultsconfirmation. The obtained results showed a significant elevation in MDA, MPO and immunologicalmarkers levels, with significant reduction in serum reduced glutathione and catalase activity in livertissue in hepatocellular-carcinogen induced rats as compared to the control group. However,administration of rats with the compounds under investigation resulted in a significant reduction ofMDA, MPO and immunological markers levels, and increased in reduced glutathione and catalase levelscompared to the carcinogenic non treated group. Various pathological alterations were observed in liverof chemically induced-carcinogenic group interestingly, results supported the protective effect of thecompounds under investigation and preserved the histological structures of liver tissues. These resultsconcluded that basic curcumin, tetrachlorocuprate-lysine and ascorbate exert chemopreventative effectagainst hepatocellular carcinoma.}, keywords = {Ferric nitrilotriacetate,Curcumin,antioxidant,hepatocellular carcinogen}, url = {https://bvmj.journals.ekb.eg/article_31357.html}, eprint = {https://bvmj.journals.ekb.eg/article_31357_a8e5d4f92c9887505c5c04782eeefe78.pdf} } @article { author = {Abd El Tawab, Ashraf and El-Hofy, Fatma and Ammar, Ahmed and Abdel Galil, Noha}, title = {Molecular Screening Of Virulence Genes In Avian Pathogenic Esherichia Coli}, journal = {Benha Veterinary Medical Journal}, volume = {30}, number = {1}, pages = {137-149}, year = {2016}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2016.31358}, abstract = {Avian pathogenic Escherichia coli (APEC) causes colibacillosis, which is one of the main causes ofeconomic losses in the poultry industry worldwide. This disease occurs only when the E. coli infectingstrain presents virulence factors (encoded by specific genes) that enable the adhesion and proliferationin the host organism. Herin, 15 E. coli strains of different serogroups isolated from birds withcolibacillosis were assigned to their phylogenetic groups and analyzed for the occurrence of 11virulence associated genes. phylogenetic typing showed that group B2 was the largest (33.3%, 5/15)followed by group B1 (26.7%, 4/15), groups A and D were similar in size (22.1%, 19/86). The virulenceprofiles showed that ompA was found in most isolates 14/15 (93.3%). The iss gene was found in13/15(86.6%). Followed by traT and iutA genes which were found in12/15 (80%). cvaC, stx2 and tsh geneswere present in 9/15(60%), 7/15(46%) and of the isolates, respectively. Only one isolate gave positiveamplification for stx1 and ibeA genes each. The hly gene was not encountered in any of the testedisolates}, keywords = {Avian pathogenic Escherichia coli (APEC),Phylogenetic analysis,Virulence genes}, url = {https://bvmj.journals.ekb.eg/article_31358.html}, eprint = {https://bvmj.journals.ekb.eg/article_31358_808ac43e63f0b6237d9eaf0eb36bb777.pdf} } @article { author = {Hussein, Samy and Esmeal, Tahya and Amin, Aziza and Sarhan, Eman}, title = {Chemopreventive effect of omega- 3 fatty acids in N-nitrosodiethylamine Induced hepatocellular carcinoma in rats}, journal = {Benha Veterinary Medical Journal}, volume = {30}, number = {1}, pages = {150-160}, year = {2016}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2016.31359}, abstract = {Liver cancer, predominantly hepatocellular carcinoma (HCC), represents a complex and fatalmalignancy driven primarily by oxidative stress and inflammation. The chemopreventive effect ofomega-3 on inflammatory markers and oxidative damage, caspase-3, antioxidant status andhistopathological alterations in hepatic tissue in N-nitrosodiethylamine (DEN)-induced HCC in malerats was investigated. To induce HCC, rats were given DEN (200 mg/kg b.wt.,i.p) three times at a 15day interval. 75 rats were divided into five equal groups. Control group, DEN group, DEN + ω3protected group received omega-3 (270mg/kg b. wt/day) orally 4 weeks before DEN injection andcontinued to 13 weeks, DEN + ω3 treated group firstly injected with DEN and orally treated with omega-3 from the 8th week till the end of the experiment (13th week) and normal -ω3 group received omega-3.Blood samples and liver tissues were collected at 13th week. DEN-induced HCC significantly decreasedSOD and CAT activities in liver tissue. However, a marked increase in liver tissue L-MDA, DNAfragmentation, caspase-3 and Nf-kB P65 and in serum AFP, IL-6 and TNF-α levels were observed inDEN injected rats. Initiation of HCC was demonstrated by histopathological examination of hepatictissue in DEN injected group. Histopathological examination confirmed the pathological improvementin the liver of rats in omega -3 protected and treated groups. In conclusion, omega-3 demonstrates antiinflammatory property by suppression of TNF-alpha and IL-6 due to inhibition of NF-kB p65 and exertsits chemopreventive effect on liver carcinogenesis. These data suggest that omega-3 exhibitedsignificant protection against DEN-induced HCC, which might be related with the enhancement of theantioxidant activity and the induction of apoptosis.}, keywords = {Hepatocellular carcinoma,omega-3,Inflammatory mediators,oxidative damage,histopathology}, url = {https://bvmj.journals.ekb.eg/article_31359.html}, eprint = {https://bvmj.journals.ekb.eg/article_31359_d20063dccf4f44f9341cfcc4acc9a086.pdf} } @article { author = {Abd El- Twab, Ashraf and El-Hofy, Fatma and Rizk, Amira}, title = {Molecular characterization of Aminoglycosoide and Tetracycline resistant Salmonella isolates causing new born ruminants diarrhea}, journal = {Benha Veterinary Medical Journal}, volume = {30}, number = {1}, pages = {161-170}, year = {2016}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2016.31360}, abstract = {Ten isolates of Salmonella species were obtained from sporadic cases of profuse diarrhea in newborn ruminants from El-Menofiya and El-Kalubia Governorates in Egypt. These isolates were as follow7 isolates from calves (Salmonella Typhimurium, Salmonella Enteritidis,two were SalmonellaSaintpaul, two were Salmonella Langeveld and Salmonella Havana) ,two isolates from lambs(Salmonella Typhimurium, Salmonella Bardo) and one isolate from goat-kids(SalmonellaEnteritidis). Salmonella isolates resistance to aminoglycosoides was 0.00% for Amikacin, 30% fortobramycin and 50% for streptomycin. Susceptibility of isolates to tetracycline was 50% fordoxycycline. Results showed a high incidence of aminoglycosoides resistance gene aadB in 100% ofthe isolates while aadA2 genes in 40% of the isolates. Incidence of tetracycline resistance genes was0.00% for tetA(B) and 80% for tetA(A). The difference between the results of this study and those fromother regions in Egypt necessitate a complete survey overall the country to make a complete and clearmap of salmonella servoars, their antibiotics susceptibility and in molecular characterization ofresistance determinants in each region}, keywords = {diarrhea,newborn ruminants,Salmonella,aminoglycosoides,tetracycline}, url = {https://bvmj.journals.ekb.eg/article_31360.html}, eprint = {https://bvmj.journals.ekb.eg/article_31360_15ca6bb82ebe9967bcadaceeffe3dc58.pdf} } @article { author = {Abd El- Twab, Ashraf and El-Hofy, Fatma and Rizk, Amira}, title = {Molecular characterization of Quinolones and β-Lactams Resistant Salmonella Serovars Determinants in Diarrheic Calves, lambs and goats-kids in the Middle of Nile Delta, Egypt}, journal = {Benha Veterinary Medical Journal}, volume = {30}, number = {1}, pages = {171-182}, year = {2016}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2016.31361}, abstract = {A total of (236 swabs) fecal samples from El-Menofiya and El-Kalubia Governorates, as sporadic casesof were subjected to bacteriological, biochemical, serotyping, sensitivity testing and PCR detection ofresistance genes for β-Lactames and Quinolones. Ten isolates of Salmonella species were identified,as 7 isolates (4.7%) from calves (Salmonella Typhimurium, Salmonella Enteritidis, Salmonellasaintpaul, Salmonella Langeveld and Salmonella Havana), 2 isolates (3.6%) from lambs (SalmonellaTyphimurium, Salmonella Bardo) and one isolate (3.3%) from goat-kids (Salmonella Enteritidis)Salmonella isolates sensitivity to β-lacatmes was 0.00% for ampicillin, penicillin G, piperacillin,cephalexin, cefoxitin, ampicillin-sulbactam, ceftazidime, 10% for ceftriaxone and 20% for amoxicillinclavulanicacid, increased to 60% and 100% for aztreonam, imipenem respectively. Susceptibility ofisolates to quinolones were 10% for ciprofluxacin, 20% to nalidixic acid, and 100% for each ofnorfloxacin and levofloxacin. PCR study showed beta-lactamase encoding gene, bla (TEM-1), wasidentified in 90% and the extended-spectrum beta-lactamase, (CIT) in 20% while bla (SHV) and acc in80% of isolates. The detection was 50%, 40%, and 0.00% for FOX, MOX, and bla (OXA-1) genes,respectively. Plasmid-mediated quinolone resistance, qnrB, qnrS were detected in 80% while aac(6')-Ib-cr, was only in 50% of the isolates. Results showed a high incidence of β-lacatmase than quinolonesresistance genes and higher isolate susceptibility to quinolone than to β-lacatmes which indicates higherefficacy and validity of quinolones. Results indicates quinolones LEV and NOR in addition to β-lacatmes imipenem are the drugs of choice for suspected salmonella cases.}, keywords = {diarrhea,calves,Salmonella,β-lacatmes,Quinolones}, url = {https://bvmj.journals.ekb.eg/article_31361.html}, eprint = {https://bvmj.journals.ekb.eg/article_31361_cb98442ae940c7956c0e782375e12029.pdf} } @article { author = {Ibrahim, Hemmat and El Sabagh, Rasha and Abou El-Roos, Nahla and Abd El Fattah, Hend}, title = {Antimicrobial effect of some essential oils on Staphylococcus aureus in minced meat}, journal = {Benha Veterinary Medical Journal}, volume = {30}, number = {1}, pages = {183-191}, year = {2016}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2016.31362}, abstract = {consumers increasingly demand of using natural preservatives as alternative to those chemical additivesthat has been questioned in last years. So, the effect of some essential oils as thyme, clove and garlic asantimicrobial agent against Staphylococcus aureus and their role in enhance shelf life of minced meatwere studied. The sensory analysis indicated significant advantages in using thyme, clove and garlic oilsin refrigerated minced beef. All used essential oils had considerable effectiveness in decreasing S. aureuscount. In addition, the results indicated that the bacterial counts decreased as the concentration of the oilincreased, accordingly, the concentration 1.5% of each oil gave the best effectiveness and the thyme oilshowed the highest action followed by clove and garlic oils.}, keywords = {Antimicrobial efficiency,Essential oils,Staphylococcus aureus,Minced meat}, url = {https://bvmj.journals.ekb.eg/article_31362.html}, eprint = {https://bvmj.journals.ekb.eg/article_31362_070189711533614399a0789cccd4fd05.pdf} } @article { author = {Abd El Tawab, Ashraf and El-Hofy, Fatma and Amaar, Ahmed and Saleh, Osama and Tolba, Heba}, title = {The effects of dietary prebiotic on phagocytic activity of innate immunity in Oreochromis niloticus}, journal = {Benha Veterinary Medical Journal}, volume = {30}, number = {1}, pages = {192-198}, year = {2016}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2016.31363}, abstract = {The objectives of this study, effects of prebiotic β polo on innate immunity of Oreochromis niloticus(O.niloticus) and increase resist against disease. A total number of 180 Nile-tilapia fish (O. niloticus),were divided into four equal groups; the first one was served as control fed on basal non-treated diet.The second group fed on diet supplemented with 1.5 ml prebiotic βpolo /kg basal fish diet, the thirdgroup fed on diet supplemented with 1 ml prebiotic βpolo/kg basal fish diet, and the fourth group fed ondiet supplemented with 0.5 ml prebiotic βpolo/kg basal fish diet for 40 days. The results showed that,significant increase in phagocytic activity test and total leucocyte count (TLC) levels. The percent levelof protection among the three treated groups after challenge infection using Aeromonus sobria Aer.sobria (0.5 ml of culture suspension of pathogen containing 107 bacteria ml-1) was higher than controland the highest record was in the group received 1.5 ml prebiotic βpolo /kg basal fish diet. The resultsof the present study support the use of 1.5 ml prebiotic βpolo /kg basal fish diet as fishimmunostimulants.}, keywords = {prebiotic,phagocytosis,Aeromonus sobria}, url = {https://bvmj.journals.ekb.eg/article_31363.html}, eprint = {https://bvmj.journals.ekb.eg/article_31363_cfc22d257e352819d50dac9ffdb7438d.pdf} } @article { author = {Abd El Tawab, Ashraf and Maarouf, Ahmed and Ahmed, Nesma}, title = {Detection of Virulence factors of Pseudomonas species isolated from fresh water fish by PCR}, journal = {Benha Veterinary Medical Journal}, volume = {30}, number = {1}, pages = {199-207}, year = {2016}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2016.31364}, abstract = {This study was conducted on 100 diseased Nile tilapia (O. niloticus) fish of various sizes collected fromdifferent fish markets in Kaliobia Governorate to estimate the prevalence of Pseudomonas infection anddetection of some virulence genes in the isolated P. aeruginosa strains. The results of bacteriologicalexamination revealed that the prevalence of Pseudomonas septicemia with Pseudomonas speciesisolation was 17.0% (17 \ 100 examined fish). These cases were attributed to P. anguilliseptica; P.aeruginosa and P. fluorescens (14/43.7%; 12/37.5% and 6/18.8%), respectively. In addition, 32Pseudomonas species were isolated, 11from liver samples (34.4%); 10 from kidney samples (31.2%); 6from gill samples (18.8%) and 5 from skin samples (15.6%). Moreover, 14 P. anguilliseptica wereisolated with an incidence of 35.7%, 28.6%, 21.4% and 14.3% followed by 12 P. aeruginosa 33.3%,25%,16.7% and 25% respectively; 6 P. fluorescens 33.3%; 50.0%,16.7% and 0.0% from the liver,kidney, gill and skin samples respectively. The in-vitro antimicrobial sensitivity test showed that theisolated Pseudomonas strains were sensitive to gentamycin; enrofloxacin; norfloxacin; ciprofloxacinand florphenicol. Meanwhile; they were intermediate sensitive for doxycycline; sulfa-trimethoprim;oxytetracycline; nalidixic acid and streptomycin. In contrast, they were resistant for cefotaxime;erythromycin; amoxicillin; methicillin; oxacillin and ampicillin. Moreover, the PCR results revealedthat, opr L and exo S virulence genes were detected in all six studied strains (100.0%). Meanwhile, phzM virulence gene was detected in 5 out of 6 studied strains (83.3%) and tox A virulence gene wasdetected in 4 out of 6 studied strains (66.7%) i.e., all studied strains were Ps. aeruginosa and all of themwere virulent strains}, keywords = {fish,Bacteriological Evaluation,Pseudomonas Species,PCR,oprL,exoS genes}, url = {https://bvmj.journals.ekb.eg/article_31364.html}, eprint = {https://bvmj.journals.ekb.eg/article_31364_04f423b00188a75a6638d4c05572411a.pdf} } @article { author = {AbouZaid, Omayma and El-sogheer, Heba and El-sonbaty, Sawsan}, title = {Evaluation of protective and therapeutic role of zinc oxide nanoparticles and aloin on dextran sulfate-induced ulcerative colitis in rats}, journal = {Benha Veterinary Medical Journal}, volume = {30}, number = {1}, pages = {208-218}, year = {2016}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2016.31365}, abstract = {This study designed to investigate the anti –inflammatory effect of zinc oxide nanoparticles (ZnONPs)and \ or aloe component (aloin) on inflammatory mediators and oxidative stress in dextran sulfatesodium salt induced ulcerative colitis(UC) in rats. sixty-four albino rats divided into eight equal of eightrats each. Group 1:(normal control) received no drugs , group 2:( ulcerative colitis) rats received dextransulfate sodium salt 3% in drinking water, group 3:( ZnONPs) rats administered ZnONPs (5mg/kg bodyweight) orally for 3weeks, group 4: (aloin ) rats administered orally with 1ml of aloin 0.1% daily for3weeks, group 5: (aloin + ZnONPs) rats orally administered with NZnO (5mg/kg body weight) and(aloin 0.1%), daily for 3weeks, group 6: (ZnONPs +UC) rats with ulcerative colitis orally treated withZnONPs (5mg/kg body weight) daily for 3 weeks, group7: (aloin+UC) rats with ulcerative colitis orallytreated with (aloin 0.1%), daily for 3weeks, group 8: (aloin+ ZnONPs +UC) rats with ulcerative colitisorally treated daily with ZnONPs (5mg/kg body weight)+aloin (1ml aloin 0.1%) for 3weeks. Theobtained results revealed that, administration of ZnONPs and\or aloin to rats with ulcerative colitissignificantly reduced elevated serum total cholesterol and TG concentrations, and markedly increasedthe reduced HDL-C level. On the other hand, elevated level of COX-2, IL-6, MDA and TNF-α in UCrats were significantly reduced, with significant increase of the reduced level of GSH. Results suggestthat ZnONPs modulates UC, while aloin showed high efficacy to normalize UC tissues and mayconsidered as potential treatment for UC and other inflammatory bowel disease.}, keywords = {Zinc Oxide Nanoparticles,aloin,ulcerative colitis,Inflammatory mediators}, url = {https://bvmj.journals.ekb.eg/article_31365.html}, eprint = {https://bvmj.journals.ekb.eg/article_31365_b8743342d47383695c2b5fb77df8ace9.pdf} } @article { author = {Abd El-Tawab, Ashraf and El Hofy, atma and Amaar, Ahmad and Sleim, Mokhtar and Salem, Heba}, title = {Molecular characterization for some virulence and antibiotic resistance genes of Staphylococcus aureus isolated from dairy cattle's subclinical mastitis in EL- Sharkia Governorate}, journal = {Benha Veterinary Medical Journal}, volume = {30}, number = {1}, pages = {219-230}, year = {2016}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2016.31366}, abstract = {Staphylococcus aureus has emerged as a significant public health problem as it is often responsible forintramammary infection in bovine. The emergence of MRSA in animals was from an outbreak ofmastitis in cattle which represent a great economic in milk industry. The present study was carriedout to genotypically characterized S. aureus isolated from subclinical bovine mastitis in different farmsin EL- Sharkia Governorate. A total of 15 S. aureus isolates were obtained from 100 subclinical mastiticmilk samples and subjected to PCR for detection of some virulence and antimicrobial resistance genesusing oligonucleotide primers that amplified genes encoding enterotoxin genes A to E (sea, seb, sec,sed, see), coagulase gene (coa), the IgG binding region of protein A (spa) and resistance gene as factoressential for expression of methicillin resistance (femA) which was used as an internal positive controland intrinsic methicillin resistance gene (mecA). PCR amplification revealed that all S. aureus isolateswere enterotoxogenic and MRSA, harbored the genes encoding staphylococcal coagulase and the genesencoding the immunoglobulin G binding region of protein A. The data in the study provided an overviewon the distribution of virulence determinants of MRSA strains which contributed to bovine mastitisproblem in the Egypt farm.}, keywords = {Staphylococcus aureus,Polymorphism,Genotyping,Virulence factors,mastitis}, url = {https://bvmj.journals.ekb.eg/article_31366.html}, eprint = {https://bvmj.journals.ekb.eg/article_31366_1e7446037bfcb6c237bf3dfe67d63204.pdf} } @article { author = {Abd El Tawab, Ashraf and Elsayed, Mai and Amaar, Ahmed and El-bialy, Amany}, title = {A study of outer membrane protein (OMPs) genes for detection of salmonella organisms in poultry farms}, journal = {Benha Veterinary Medical Journal}, volume = {30}, number = {1}, pages = {231-237}, year = {2016}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2016.31367}, abstract = {Bacteriological examination of internal organs obtained from diseased layers, broilers and baby chicksrevealed isolation of salmonellae with an incidence 5%, 2.5% and 7.1%, respectively. ElevenSalmonella isolates were recovered out of 250 poultry samples. The highest percentage rate was in liver4%, followed by intestine 2.4% and finally spleen 0.8%. Salmonella organisms were recovered fromovary of layers with an incidence 3.33%. Serological identification of Salmonella isolates revealed thatthe most prevalent serovars was. Enteritidis (3) followed by. Gallinarum (2) and Rissen (2) then S.Kentukey, S. Florida, S. Lomita and S. Sontheim (one isolate for each). The goal of this study was toevaluate the suitability of the outer membrane genes OmpA and OmpF for detection of GenusSalmonella in clinical samples. OmpA gene was detected in 100% of the examined clinical sampleswhile OmpF gene was detected in 14 clinical samples. Three samples were positive by OmpF.PCR morethan cultural method. It was concluded that PCR protocol decrease the time needed for the detection ofSalmonella OmpA gene couldn't discriminate genus Salmonella from other non-Salmonella organismsin clinical samples .AS it amplified OmpA in other Enterobacteriaceae. However, OmpF gene ispromising tool for detection of Genus Salmonella}, keywords = {S. enteritidis,outer membrane protein genes,PCR}, url = {https://bvmj.journals.ekb.eg/article_31367.html}, eprint = {https://bvmj.journals.ekb.eg/article_31367_458bf850423c0bf3b107207d763bf66d.pdf} } @article { author = {Abdel-Tawab, Ashraf and El-Hofy, Fatma and Maarouf, Ahmed and Abbas, Sameh}, title = {Molecular detection of some virulence genes of S. aureus isolated from mastitic Cows by PCR}, journal = {Benha Veterinary Medical Journal}, volume = {30}, number = {1}, pages = {238-245}, year = {2016}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2016.31368}, abstract = {In this study a total of 1200 quarter milk samples were selected from 300 cows at different localities ofEl-Kaliobia Governorate were examined bacteriologically and revealed that 57 S.aureus isolated fromboth clinical and subclinical mastitis with incidence of 45.6% of total isolates. Molecular typing of S.aureus by PCR revealed that all isolates (100%) (12 random S.aureus isolates) were positive for the 16SrRNA genes of S. aureus. Genotyping of virulence genes encoding factors of S. aureus revealed thattwo isolates were positive for (tst-1) gene (16.7%), three isolates positive for (etb) 25%, nine isolatespositive for each of (icaD and femA) genes (75%), seven isolates positive for (hlg) (58.3%), eight isolatespositive for (nuc) (66.7%).}, keywords = {mastitis,S. aureus,PCR,Virulence genes}, url = {https://bvmj.journals.ekb.eg/article_31368.html}, eprint = {https://bvmj.journals.ekb.eg/article_31368_cbcf41c26faa0e1a31cc2816faa0de3a.pdf} } @article { author = {Mohammed, Hamdi and Abdou, Adham and Eid, Amal and Zakaria, Asem}, title = {Rapid tests for detection of ciprofloxacin residues in liquid milk}, journal = {Benha Veterinary Medical Journal}, volume = {30}, number = {1}, pages = {246-253}, year = {2016}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2016.31369}, abstract = {A total of 234 randomly selected milk samples from different sources (110, 50, 34 and 40 samples) fromeach of (market milk, collecting centers, bulk tank and UHT milk samples; respectively) were analyzedfor the quinolones residues by rapid quinolones detection strip test. Results revealed that 17.3%, 14%and 20.6% of milk samples were positive for the presence of quinolones residues for market milk,collecting centers and bulk tank milk samples; respectively, while not detected in UHT milk samples.The positive milk samples were subjected to HPLC analysis for quantitative detection of ciprofloxacinresidues, which were present in 11.8%, 8%, and 8.8% for market milk, collecting centers and bulk tankmilk samples; respectively, with mean values of 17.37± 0.27, 18.02±0.7 and 17.79± 0.75 ppb;respectively. None of them had a concentration exceeded the maximum residual limit set by differentinternational standards. Public health importance of ciprofloxacin residues was discussed}, keywords = {Rapid Detection,ciprofloxacin,Liquid Milk}, url = {https://bvmj.journals.ekb.eg/article_31369.html}, eprint = {https://bvmj.journals.ekb.eg/article_31369_e860e1b6709f01fbcd4d2e02d63b2f4e.pdf} } @article { author = {A., Marzouk and M.A., Ashoub and F., Metawea and S., Azam and H., Mansour}, title = {Persistence of deltamethrin and diazinon in environment of dairy farm}, journal = {Benha Veterinary Medical Journal}, volume = {30}, number = {1}, pages = {254-259}, year = {2016}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2016.31370}, abstract = {The present study was carried out to investigate the persistence of some pesticides (diazinon anddeltamethrin) in water, feeds and milk. The obtained results illustrated that diazinonwas persisted forlonger time in water, feed and milk samples than deltamethrin, it disappears within 21st and14th day afterapplication in water and feed, respectively, while it disappears within 30th day after application in milk.Moreover, deltamethrin disappear within 7th day after application in feed and water, while in milk itdisappears within 21st day after application. We concluded that diazinon and deltamethrin were persistedin milk for longer time than both feed and water. Pesticides contamination of milk and milk productsmay lead to acute or chronic toxicity for human being representing public health hazards through theircumulative effects}, keywords = {Diazinon,Deltamethrin,persistence,Milk}, url = {https://bvmj.journals.ekb.eg/article_31370.html}, eprint = {https://bvmj.journals.ekb.eg/article_31370_95cd7895669d545f14299a9902793475.pdf} } @article { author = {Mahfouz, Mohamed and Youseif, Mahmoud and El-Mashad, Abdel-Baset and Shendy, Mohamed}, title = {Biochemical effects of ear infections by Pseudomonas aeruginosa on rabbits}, journal = {Benha Veterinary Medical Journal}, volume = {30}, number = {1}, pages = {260-265}, year = {2016}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2016.31371}, abstract = {Ear infections (particularly, otitis media) is a prevailing and common infection in developing countriescausing local damage and threatening complications. Pseudomonas aeruginosa is the most commonpathogen causing chronic suppurative otitis media (CSOM) and malignant otitis externa. The objectiveof this study is to identify incidence of Pseudomonas aeruginosa involved in ear infections andassociated biochemical parameters which may be changed. External auditory canal in rabbit ears wasinoculated with (106) colony-forming units (CFU) of Pseudomonas aeruginosa or left as sterile controlsfor eight weeks. There are significantly decrease results at (P<0.05) of Interleukin-2 and significantincrease results of Interleukin-9 and Tumor Necrosis Factor-alpha (TNF-α) in acute or chronicinfections. Moreover, histopathological changes of ear, kidney and liver tissues were also observed.Evaluation of these parameters were needed to use of specific blocking agents to inflammatorymediators with the aim of discovering new treatment options for chronic otitis media, and to help thephysicians in diagnosis these diseases in elderly stages and try to reduce the pain and suffering associatedwith otitis media.}, keywords = {Otitis Media (OM),Pseudomonas aeruginosa,Interleukins}, url = {https://bvmj.journals.ekb.eg/article_31371.html}, eprint = {https://bvmj.journals.ekb.eg/article_31371_2f500b5a806604eca943df6a3eb13079.pdf} } @article { author = {Elkomy, Ashraf and Aboubakr, Mohamed and Medhat, Nermin}, title = {Some teratological effects of difloxacin in rats}, journal = {Benha Veterinary Medical Journal}, volume = {30}, number = {1}, pages = {266-271}, year = {2016}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2016.31373}, abstract = {The aim of this experiment was to detect the teratogenic effects of difloxacin in Wister Albino rats.Thirty pregnant rats were divided into 3 groups; group (1) behaved as control and received normal saline.Group (2) was administered difloxacin at a dose of 20 mg/kg b. wt and group (3) was administereddifloxacin at a dose of 40 mg/kg b. wt orally once daily from 6th to 15th day of pregnancy. The studyrevealed no teratogenic effect at a dose of 20 mg/kg b. wt, while at a dose of 40 mg/kg b.wt hassignificant teratogenic effect showed by decrease in size, weight, length, retarded growth in fetuses, fetalresorption of treated groups, the incidences of skeletal & visceral abnormalities were increased in treatedgroup (40 mg/kg b. wt). It could be concluded that. Administration of difloxacin during early stage ofpregnancy and at high dose induce some fetal abnormalities.}, keywords = {difloxacin,Pregnancy,Visceral and Skeletal Abnormalities}, url = {https://bvmj.journals.ekb.eg/article_31373.html}, eprint = {https://bvmj.journals.ekb.eg/article_31373_df09f94525486172ee48e51c709f80b5.pdf} } @article { author = {Ali, Hussein and Mahfouz, Mohamed and Abd-elwahab, Badran and Ahmed, Adel}, title = {Biochemical effects of intestinal surgery in gastrointestinal enzyme}, journal = {Benha Veterinary Medical Journal}, volume = {30}, number = {1}, pages = {272-282}, year = {2016}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2016.31376}, abstract = {This study was performed to investigate the effected of experimental work of intestinal surgery forremoval caecum of rabbits on alanine amiotransferase (ALT), aspartate aminotransferase(AST), gammaglutamyletransferase (GGT), alkaline phosphatase (ALP), acid phosphotase (ACP), amylase, lipase,catalase (CAT), ceratine phosphokinase (CPK), lactate dehydrogenase (LDH) and cortisol hormone.Two groups of thirteen healthy female New Zland rabbits were used. The first one contained 15 rabbitsand kept as control group. The second contain also 15 rabbits subjected to surgery for removal of caecumof rabbits. Blood sample were collected at 3, 6 and 9 days post-operative. The obtained result showedthat the increase in serum ALT, AST, GGT, ALP, ACP, amylase, lipase, CAT, CPK, LDH and cortisolhormone were significant increase in experimental group compared with control group. From this resultit could be concluded that the biochemical parameter affected with intestinal surgery for removal ofrabbit’s caecum}, keywords = {Rabbits,Caecum,Surgery}, url = {https://bvmj.journals.ekb.eg/article_31376.html}, eprint = {https://bvmj.journals.ekb.eg/article_31376_33de20dc66eb5454472ffaaa432063df.pdf} } @article { author = {El-Boroaay, Ibrahiem and Abd El Kader, Samer and Shaheen, Adel}, title = {Dual effect of Aspergillus Fumigatus and E. coli O125 in broilers}, journal = {Benha Veterinary Medical Journal}, volume = {30}, number = {1}, pages = {283-294}, year = {2016}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2016.31377}, abstract = {This study was conducted on 88 broiler chicks from one day to five days. They were collected fromthree hatcheries at two governorates to detect the prevalence of infection by Aspergillus spp and/or E.coli serotypes. This study revealed that the prevalence was 35.2%,43.2% and 18.2%for E. coliserotypes, Aspergillus spp and both infection together, respectively. The clinical signs of the examinedbroiler chicks appeared as weakness, respiratory manifestation (gasping) and diarrhea. The postmortemlesions were mainly on lungs and air sacs which appeared congestion, white nodules in lungs andyellowish white cheesy materials on air sacs and lungs. Some chicks showed signs of enteritis.Bacteriological and mycological examinations for collected broiler chicks revealed that E. coli O125and Aspergillus fumigatus were the most common isolates. Experimental infection of 124 one-day oldbroiler chicks which checked and confirmed to be free from both infectious agents with inoculation ofE. coli O125 (intranasal/ one dose) and/or Aspergillus fumigatus (intra air sacs/one dose) showedweakness, respiratory manifestation(gasping), diarrhea, congested lungs and cheesy materials on lungsand air sacs. Reisolation and identification revealed positive results for infectious agents.Histopathological examination was also described. Concerning to growth performance and mortalityrate, dual infection showed much decrease in weight and increase in mortality rate than single infection.In conclusion the dual infection has more pathogenic effect than single infection.}, keywords = {Aspergillus fumigatus,E. coli,Broilers,intranasal and intra air sacs inoculation}, url = {https://bvmj.journals.ekb.eg/article_31377.html}, eprint = {https://bvmj.journals.ekb.eg/article_31377_e162fb3f7fcccef98557e0a85fbcfe1b.pdf} } @article { author = {Salem, Amani and Amin, Reham and Khater, Dalia and Shokr, Loaloa}, title = {Antifungal Effect of Some Chemical Preservatives on Aspergillus Niger in Minced Beef Meat}, journal = {Benha Veterinary Medical Journal}, volume = {30}, number = {1}, pages = {295-301}, year = {2016}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2016.31399}, abstract = {A grand total of 1400 g fresh minced beef meat samples were divided into 7 equal groups (200 g ofeach) and then irradiated. Aspergillus niger was inoculated into each group with infective dose 105cfu/g. The used chemical preservatives natamycin (100, 200 and 300 ppm) and potassium sorbate (0.1%,0.2% and 0.3%) were added. The inoculated samples were stored at 4oC in refrigerator till be used. Theinoculated groups were examined every 24 hrs for A. niger count and sensory examination (overallacceptability). The experiment was performed in triplicate. Natamycin (100, 200 and 300 ppm)decreased count of A. niger (log cfu/g) from 5.00 (initial load) to 2.83, 2.79 and 2.76 with reductionpercentages 43.40%, 44.20% and 44.80% on 8th, 8th and 10th days of storage, respectively. Potassiumsorbate (0.1%, 0.2% and 0.3%) decreased count of A. niger (log cfu/g) from 5.00 (initial load) to 3.02,2.97 and 2.85 with reduction percentages 39.60%, 40.60% and 43.00% on 8th, 8th and 10th day ofstorage, respectively. While, in control group A.niger count increased From 5.00 (initial load) to 5.56on 6th day Furthermore, natamycin (300 ppm) treated minced meat samples artificially inoculated withA. niger showed overall acceptability till 8th day of storage. In comparison, potassium sorbate (0.3%)showed overall acceptability till 7th day. While, control group showed overall acceptability till 4th day.Generally, natamycin (300 ppm) proved to be more efficient than potassium sorbate (0.3%) insuppression of A. niger growth in minced meat. So, the use of natamycin (300 ppm), as it is safeantifungal agent, is therefore recommended to improve safety of the meat products.}, keywords = {Aspergillus niger,antifungal effect,meat products,food preservative}, url = {https://bvmj.journals.ekb.eg/article_31399.html}, eprint = {https://bvmj.journals.ekb.eg/article_31399_f76e043ba29772a9071f95c0311b5d79.pdf} } @article { author = {L., Sayed and S., Shell and Ahmed, Hanan and Ibrahim, Hanan and M., Ali and A., Nasr}, title = {Efficacy of a locally prepared bovine mastitis vaccine}, journal = {Benha Veterinary Medical Journal}, volume = {30}, number = {1}, pages = {302-311}, year = {2016}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2016.31400}, abstract = {The objective of the present work is to prepare and evaluate the effectiveness of a locally preparedpolyvalent vaccine against mastitis from the most common causes of mastitis. Staphylococcus aureus,Streptococcus agalactiae and Escherichia coli were the most prevalent bacteria recovered from clinicaland subclinical mastitis. MontanideISA-206 adjuvanted inactivated polyvalent vaccine containing thethree strains was prepared. Twenty pregnant cows were inoculated intramuscularly with the preparedpolyvalent vaccine two months prior to calving and boostering at day 21 from the primary injection.Serum samples from vaccinated and non-vaccinated cows were collected at the 1st, 3rd, 8th, 12th, 16th, 20thand 24th weeks post vaccination and evaluated immunologically using ELISA. The results showed thatimmune response was significantly higher in the vaccinated group than that of controls. These resultscould be indicated the safety and effectiveness of the vaccine in reduction of incidence and severity ofclinical cases of mastitis but further studies should be done to elucidate the possibility of field applicationand effectively}, keywords = {mastitis,vaccine,S. aureus,S. agalactiae,E. coli}, url = {https://bvmj.journals.ekb.eg/article_31400.html}, eprint = {https://bvmj.journals.ekb.eg/article_31400_71772ca9e045e4f37e062f93ad606edc.pdf} } @article { author = {A., Marzouk and Ashoub, Mona and F., Metawea and Azam, Aya and H., Mansour}, title = {Seasonal variations on the levels of some pesticide residues in dairy farms}, journal = {Benha Veterinary Medical Journal}, volume = {30}, number = {1}, pages = {312-322}, year = {2016}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2016.31401}, abstract = {The present work was carried out to study the effect of seasonal variations on the levels of pesticideresidues in water, feeds and milk. Our results illustrated that the significantly highest concentrations ofOCs in water and feed were detected during summer [(1.05±0.35, 0.72±0.24, 0.77±0.25, 2.5±0.70 and0.81±0.27 μg/L, for p.p.DDT, aldrin, β-BHC, lindane and γ.chlordane respectively in water samples)and (30.0±10.20, 45.00±13.02, 65.33±21.90, 35.33±13.20, 43.67±13.30, 22.00±7.33 and 19.13±8.6 μg/kg, for p.p.DDD, p.p.DDE, p.p.DDT, aldrin, lindane, γ.chlordane and methoxychlor, respectively infeed samples) ], while the lowest were detected during spring, on the other hand, none of pesticidesdetected in winter which may be attributed to environmental factors. The significantly highestconcentration of diazinon in water and feed was recorded during autumn (78.75±26.30 μg/L and140.00±64.60 μg/kg, respectively), while the lowest levels were detected during spring. Also, thehighest concentrations of deltamethrin was detected during spring in water (55.30±18.50 μg/L) andduring summer in feed samples (78.40±26.70 μg/kg), while the lowest levels were detected duringautumn in water and during spring in feed samples. The significantly highest concentrations of residuesin milk were detected during summer (p.p.DDD, p.p. DDE, p.p.DDT, heptachlor, heptachlor epoxide,γ.chlordane, methoxychlor, diazinon and deltamethrin were 20.10±6.01, 52.30±17.20, 33.20±20.06,1.16±0.38, 6.72±0.90, 2.570±2.23, 0.70±0.20, 58.50±19.20 and 45.13±15.10 μg/kg, respectively) andthe lowest were detected in winter. Our results concluded that the seasonal variations greatly influencethe levels of all examined pesticide residues with variable degree specially diazinon and deltamethrin.}, keywords = {Seasonal variations,OCs,Diazinon,Deltamethrin,Milk,water,feed}, url = {https://bvmj.journals.ekb.eg/article_31401.html}, eprint = {https://bvmj.journals.ekb.eg/article_31401_1a63cdca4c8cb786f8aa122b32682f5d.pdf} } @article { author = {Armany, George and Ahmed, Hanaa and Ibrahim, Hemmat and Amin, Reham}, title = {Detection of some foodborne pathogens in meat products by Polymerase Chain Reaction}, journal = {Benha Veterinary Medical Journal}, volume = {30}, number = {1}, pages = {323-330}, year = {2016}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2016.31402}, abstract = {A total of 100 random samples of meat products including raw minced meat, raw sausage, luncheon andbasterma (25 samples of each) were collected from different markets in Cairo and Giza governorates tobe examined bacteriologically for detection of Staphylococcus aureus, Listeria monocytogenes andEscherichia coli. These samples were examined for isolation of such pathogens by conventionalbacteriological methods and by polymerase chain reaction (PCR). Concerning S.aureus bacteriologicalresults revealed the prevalence in minced meat, Sausage, luncheon and basterma was (24%, 24%, 20%,4%) respectively. While L. monocytogenes revealed the prevalence in minced meat, sausage, luncheonand basterma was (4%, 0%, 0%, 0%) respectively and E. coli revealed the prevalence in minced meat,sausage, luncheon and basterma was (20%, 20%, 24%, 20%) respectively. The results cleared that PCRis an ideal method for identification of foodborne pathogens, as it was effective, less labor, moresensitive, reduces effort and time after using gradient PCR in validation of each microbe.}, keywords = {Foodborne pathogens,Optimization,polymerase chain reaction (PCR),bacteriological isolation,meat products}, url = {https://bvmj.journals.ekb.eg/article_31402.html}, eprint = {https://bvmj.journals.ekb.eg/article_31402_4127b5ea6c63e2bf769f2324f13b8421.pdf} } @article { author = {Khedr, Nasser and Ahmed, Tahia and Ragab, Fatma}, title = {Effects of Sunflower Meals Inclusion on Performance and Some Haematological Parameters in Broiler chicks}, journal = {Benha Veterinary Medical Journal}, volume = {30}, number = {1}, pages = {331-340}, year = {2016}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2016.31403}, abstract = {A total of two hundred twenty five one-day-old Indian river broiler chicks (IR) were distributed intofive different treatment groups (A, B, C, D and E), of three replicates (15 chicks / replicate). The chicksin control group (A) were fed control diet with β- mannanase (Hemicell®), groups B and C were feddiets containing SFM (2.5 and 5%, respectively) without β-mannanase (Hemicell®). While groups Dand E were fed diets containing SFM (2.5 and 5%, respectively) with β-mannanase (Hemicell®). Effectsof inclusion Sunflower meals with or without β-mannanase (Hemicell®) on performance showed thatgroups containing Sunflower meals 2.5%with or without enzyme show improvements than other groups.Erythrogram profile showed a significant (p≤0.05) difference between control group and other groupsin Hb, RBCs, PCV and MCV levels, while there was a significant (p≤0.05) increase in MCH and MCHCin group E (SFM 5%+Hemicell®) when compared with other groups. Leukogram showed nonsignificantchanges between control group and other groups. It was concluded that inclusion ofsunflower meals 2.5% had a positive role in improvement final body weight changes and final bodyweight gain. Partial replacement diet with sunflower meals (2.5 and 5% with or without Hemicell®) didnot induce side effects on hematological parameters}, keywords = {broiler chicks,Sunflower meals,β-mannanase (Hemicell®),Chick performance and hematological profile}, url = {https://bvmj.journals.ekb.eg/article_31403.html}, eprint = {https://bvmj.journals.ekb.eg/article_31403_92af1b7841d53af30423bbec752f6131.pdf} } @article { author = {Aidaros, Hassan and Ashoub, Mona and El Daous, Hala and Fakhry, Hiam}, title = {Effect of some factors on prevalence of FMDV antigen in both cattle and buffaloes in Egypt}, journal = {Benha Veterinary Medical Journal}, volume = {30}, number = {1}, pages = {341-347}, year = {2016}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2016.31404}, abstract = {The present study was carried out for detection and identification of the isolated FMDV from infectedtissue samples using serotyping antigen detection ELISA kit (IZSLER ELISA Kit).Thirty six lesionsamples were collected from different localities (Menofia, Gharbia, Kalyoubia, Giza and Sharquiagovernorates) during the period from May 2014 to July 2015.The obtained results indicated thatclinically, unvaccinated infected cattle in Gharbia governorate 2014were positive to FMDV infectionserotype O. Samples collected from infected, vaccinated cattle and buffaloes in Kalyoubia governorateduring 2015 were positive to FMDV infection serotype O. Clinically infected cattle and buffaloes inMenofia governorate during 2015 seven samples were positive to FMDV infection, which serotyped asfive samples were serotype O & two samples were SAT-2. Clinically infected cattle and buffaloes inGiza governorate during 2015 sixteen samples were positive to FMDV infection, which eight samplesfrom them serotyped as SAT-2 & seven samples from them were serotype O & one of them wasserotyped A. Clinically infected un vaccinated cattle in Sharquia governorate 2015 three samples werepositive to FMDV infection serotype A. We concluded that large number of both vaccinated and unvaccinated animals in these governorates affected by FMDV infection by its different serotypes in theperiod of the study, and there are different factors affecting prevalence of FMDV as (age, state ofvaccination, type of vaccination, sex, season and species).}, keywords = {FMD,Antigen detection ELISA,tongue epithelium}, url = {https://bvmj.journals.ekb.eg/article_31404.html}, eprint = {https://bvmj.journals.ekb.eg/article_31404_5bcb585e77f608b370370cfd6bc2d1f3.pdf} } @article { author = {Hassanen, Fatin and A., Kamel and Gaafar, Rehab and A., Shaheen}, title = {Effect of Grilling on Pesticides Residues in O. niloticus Muscles}, journal = {Benha Veterinary Medical Journal}, volume = {30}, number = {1}, pages = {348-357}, year = {2016}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2016.31405}, abstract = {Thirty six random healthy Oreochromusnilotica (O. niloticus) collected from Manzala Lake, wild NileRiver "El-Ryiah El-Tawfiky", El-Abbasa private fish farm and Gesrbahr el-baar agricultural drainage(9 fish from each source). Concentrations of organochlorine pesticide residues (OCPs) were investigatedin both raw and grilled fish (dry heat treatment). Fish musculature samples were analysed for detectionthe effect of grilling on level of organochlorine pesticides residues. Examination revealed that, samplescollected from El-Ryiah El-Tawfiki recorded the highest concentration of organochlorine pesticideresidues in raw O. niloticus followed by Manazla Lake, El-Abbasa fish farm and Gesrbahr el-baaragricultural drain, respectively. Application of grilling revealed a great reduction in organochlorinepesticides residues concentrations by ratio of 11% to 100% depending upon the type of pesticide residue,its sensitivity to heat and locality of fish collection. In some cases thermal treatment showed unexpectedresults represented by appearance of some residues which not detected in raw samples. This studyconcluded that fish from some water resources in Egypt is highly contaminated with organochlorinepesticide residues which greatly supposed to come from agricultural contaminated water. Moreover,grilling is a good and highly recommended method to reduce organochlorine residues with unevendegrees in fish muscles before consuming.}, keywords = {Organochlorine pesticide residues,O. niloticus,fish muscles,grilling heat treatment}, url = {https://bvmj.journals.ekb.eg/article_31405.html}, eprint = {https://bvmj.journals.ekb.eg/article_31405_332ef9545c921ce12ff157973fa6d79f.pdf} } @article { author = {Hussien, Abdel-Maksoud and Ahmed, Mahmoud and Hussein, Mohammed and Gobba, Naglaa}, title = {Biochemical Study on antioxidant after High Effort}, journal = {Benha Veterinary Medical Journal}, volume = {30}, number = {1}, pages = {358-367}, year = {2016}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2016.31406}, abstract = {The present study was designed to evaluate hepatoprotective effects of Cranberry extract (75 and150mg/kg.b.w) against Diclofenac sodium induced liver toxicity in rats. Oral administration ofDiclofenac sodium (150mg/kg.b.w.) led to significant increase in plasma Transaminases (L-Alanine andL-Aspartate), Lactate dehydrogenase (LDH), Alkaline phosphatase (ALP), Tumor Nicroses Factor-Alfa(TNF-α), Nitric oxide (NO) and TBARS as well as plasma, Triacylglycerol, Total Cholesterol, and LDLCholesterol.Also, treatment of rats with Diclofenac sodium led to significant decrease in liver GSH,Protein Thiols (Pr-SHs), Superoxide Dismutase (SOD), Catalase (CAT) as well as plasma HDL. Theobtained result revealed that cranberry extract (75 and 150mg/kg.b.w.) prevent liver tissue damagethrough increasing of GSH, SOD and CAT activities and decrease significantly TBARs level. Theseresults suggest that, cranberry may be effective in enhances the protection of heart toxicity by its radicalscavenging effect and antioxidant activity}, keywords = {diclofenac sodium,liver toxicity,cranberry,Antioxidant enzymes,Lipid profile,GSH}, url = {https://bvmj.journals.ekb.eg/article_31406.html}, eprint = {https://bvmj.journals.ekb.eg/article_31406_7532955277432eb6790aeba18c736e80.pdf} } @article { author = {Ragab, Omayma and El- Senousy, Yakout and Badawy, Abdel Fattah and Rashad, Ahmed}, title = {Molecular and biochemical study on the potential therapeutic effect of novel composite on the hepatocellular carcinoma-induced rats}, journal = {Benha Veterinary Medical Journal}, volume = {30}, number = {1}, pages = {368-379}, year = {2016}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2016.31407}, abstract = {The hepatoprotective effect of basic curcumin and bis (tetrachlorocuprate -lysine) combined withascorbate against ferric nitrilotriacetate (Fe-NTA) and chloroform induced hepatic carcinogenesis in ratswas evaluated. One hundred rats were divided into five equal groups. Normal control group,carcinogenic (Fe-NTA: 9 mg Fe/kg b.wt. i.p.) and chloroform (150 mg/ kg b.wt. orally)-induced group,curcumin group (400 mg/kg. b.wt. orally), tetrachlorocuprate-lysine (25 mg/kg. b. wt. s.c.) and ascorbate(500 mg/kg. b. wt. orally) group and a mixture group (composed of curcumin, tetrachlorocuprate -lysineand ascorbate). Blood samples and liver tissue specimens were collected at the end of experiment (4months) for determination of the following parameters: activities of serum malondialdehyde (MDA),reduced glutathione (GSH), tissue catalase, myeloperoxidase (MPO), Tumor necrosis factor-α (TNF- α), interleukin-1, nuclear factor kappaB65 (NFkB65) and caspase-3. The obtained results showed thatinjection of carcinogen caused a significant elevation in the MDA, MPO and immunological markerslevels, and significant reduction in the reduced glutathione, and catalase compared to the control group.Compared to carcinogenic group, rats administered with the treated compounds resulted in a significantreduction of MDA, MPO and immunological markers levels, and increased in reduced glutathione andcatalase activity. These results concluded that basic curcumin, tetrachlorocuprate-lysine and ascorbateexert chemopreventative effect against hepatocellular carcinoma}, keywords = {Ferric nitrilotriacetate,Curcumin,Hepatoprotective,antioxidant,Carcinogen}, url = {https://bvmj.journals.ekb.eg/article_31407.html}, eprint = {https://bvmj.journals.ekb.eg/article_31407_b34fefc4ccbcb52fb6473c286934b179.pdf} } @article { author = {Abo-Ahmed, Ahmed and Lee, Dong-soo and Osawa, Masatake and Han, Xujun and Nojebuzzaman, Md and Farrag, Foad and Attia, Mohamed and Bahgat, Hatem and Kassab, Ahmed}, title = {Generating LacZ-reporter transgenic mice to identify α1 (XIX) Collagen (Col19a1) expression in Dermal Papilla Cells}, journal = {Benha Veterinary Medical Journal}, volume = {30}, number = {1}, pages = {380-395}, year = {2016}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2016.31408}, abstract = {It is widely accepted the concept that tissue morphogenesis is mediated by reciprocal interactionsbetween epithelial and mesenchymal cells, whereas the molecular details of these interactions remainlargely elusive. The hair follicle (HF) is a mini-organ whose proper morphogenesis is governed by aseries of interactions between epidermal and dermal cells. Due to its relatively simple structure, the HFaffords an excellent model to decipher the molecular mechanisms of epithelial – mesenchymalinteractions. To explore the molecular mechanisms by which Dermal Papilla (DP) cells regulate hairfollicle formation, we performed a comparative transcriptome analysis and identified numerous genespreferentially expressed in the DP cells. Among these, a gene encoding type XIX Collagen (Col19a1)has become the focus of our attention because of its extensive evolutionary conservation. We generatedCol19a1LacZ reporter mice using CRISPR/Cas9 system to induce homologous recombination torecapitulate Col19a1 expression pattern. In mouse embryos, Col19a1LacZ expression is confined to theDP cells of hair follicles, ring sinus of whiskers, skeletal muscles and basal keratinocyte layer of skinepidermis of limbs and tail. Moreover, Col19LacZ started to be expressed at E14.5 in the dermalcondensates of hair germ stage to the DP cells of mature hair follicle but not in the placode stage.Ongoing generation of Col19a1 null mice will ultimately explore the functional role of Col19a1 duringHF morphogenesis. From this perspective, it is plausible to expect that future studies will provideadditional functional evidence for Col19a1 during development which might be a useful tool tounderstand the molecular mechanisms undergoing HF stem cell regulation}, keywords = {mesenchymal,morphogenesis,Col19a1,Dermal papilla,CRISPR/Cas9,Evolutionary}, url = {https://bvmj.journals.ekb.eg/article_31408.html}, eprint = {https://bvmj.journals.ekb.eg/article_31408_ced7c6e955489759f5caec94303dc3de.pdf} }