@article { author = {Rashad, Dina and Kandiel, Mohamed and Agag, Mohsen and El-Khawagah, Ahmed and Mahmoud, Karima and Ahmed, Y.F and Abou El-Roos, mahmoud and Sosa, Gamal}, title = {Histomorphometry of Dromedary Camel Epididymis and its Correlation with Spermatozoa Characteristics during their Epididymal Transport}, journal = {Benha Veterinary Medical Journal}, volume = {35}, number = {1}, pages = {1-11}, year = {2018}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2018.35823}, abstract = {This study aimed to assess the relation between the morphometric and histological attributes of the epididymis, and epididymal semen features in mature dromedary camels. The testes with the attached epididymis (n=50) were collected from adult camels along the rutting season from December to April. The separated epididymis was evaluated for weight and length prior to the dissection. Samples from each epididymal segment were processed for histomorphometric examination. Epithelial height, luminal diameter, tubular diameter, stereocilia height, muscular coat thickness and histogram of the intra luminal content were measured. The harvested epididymal semen was evaluated for motility, concentration and livability. The intra-luminal cellular contents (histogram in pixels), epithelial and stereocilia heights, and muscular coat thickness were maximal in the epididymal head. The epididymal tail showed wider luminal and tubular diameters than head and body. The tubular diameter and histogram were positively correlated (p<0.05) with sperm motility in the head and body segments.The length of head epididymal segment was negatively correlated with sperm concentration. Epithelial height, stereocilia height were negatively correlated with sperm motility in epididymal body. In conclusion, the histomorphometry aspects of the epididymis eminently impacts spermatozoa features in dromedary camel.}, keywords = {camel,epididymis,histomorphometry,Rutting season,Spermatozoa}, url = {https://bvmj.journals.ekb.eg/article_35823.html}, eprint = {https://bvmj.journals.ekb.eg/article_35823_18fb551d988b0470b48e2f0ea577d8ef.pdf} } @article { author = {Abd El Tawab, Ashraf and Agag, Mohsen and Khoudair, R.M. and Anwer, Basma}, title = {Some studies on Brucella among camels with reference to isolated strains}, journal = {Benha Veterinary Medical Journal}, volume = {35}, number = {1}, pages = {12-21}, year = {2018}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2018.35840}, abstract = {A number of 271 apparently healthy camels of both sexes at different ages were used in this study during a period from April 2016 to May 2017. Samples (271 serum samples, 30 milk samples , 21 tissue specimen and lymph nodes) were obtained from slaughtered camels in different localities (Cairo, Giza, El Sharkyia, El Behira, Matroh). Three serological tests were applied including Buffered Acidified Plate Test (BAPT), modified Rose Bengal Plate Test (mRBPT), and Tube Agglutination Test (TAT). The prevalence of reactors for brucellosis was 9.5%, 8.8% and 7.7% respectively. All samples confirmed by using Rivanol Test (Riv.T) and Immunochromatographic assay (ICA) with 8.5% and 9.2% prevalence respectively. All collected milk samples were Negative by MRT (0/30). ICA or (LFA) is highly sensitive, accurate and specific diagnostic assay since it directly detects antibodies of Brucella organism and is considered as rapid confirmatory test. Culturing from 21 tissue samples and L.N revealed positive isolates as 5/21 (23.8%). The isolated strain was identified biochemically and by Polymerase Chain Reaction (PCR) with 498 bp and revealed predominance of Brucella abortus in all isolates as 5 (100%). This work aims at providing an overview on diagnostic investigations, as brucellosis has an economic impact on the production and reproduction in camels.}, keywords = {Brucella,camels,BAPT,mRBPT,TAT,Riv.T,ICA,MRT}, url = {https://bvmj.journals.ekb.eg/article_35840.html}, eprint = {https://bvmj.journals.ekb.eg/article_35840_96f0d24bffd40fdb4de650c6b98a260e.pdf} } @article { author = {Abdel-Tawab, Ashraf and Agag, Mohsen and Hamdy, Mahmoud and Wareth, Gamal and El-Hofy, Fatma and Ramadan, Mohamed}, title = {Genotypic characterization of Brucella Spp.isolated from sheep and goats}, journal = {Benha Veterinary Medical Journal}, volume = {35}, number = {1}, pages = {22-29}, year = {2018}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2018.35938}, abstract = {Brucellosis is a major infectious zoonotic disease affecting animal and human.In animal, the disease is causing great economic losses such as abortion, still birth as well as infertility in both male and female.A cross sectional study carried out from November 2016 to May 2017 to estimate the seroprevalence of brucellosis in sheep and goatsin Benha.A total of 38 animals (20 sheep, 18 goats)were investigated. 19 serum samples (8 from sheep and 11 from goats) subjected for serological examination. The results showed that,5/8(62.5%) and 9/11(81.8%) from sheep and goats, respectively, were positive by Rose Bengal Plat test(RBPT), Buffered Acidified Plat Antigen test(BABA)and Rivanol test (RIV test).Atotal of 34 lymph nodes from seropositive sheep and goats were examined bacteriologically. 3/20 (15%) and 4/14 (28.57%) were culture positive for Brucellarespectively. None of vaginal swabs revealed culture positive results.All isolates were confirmed genetically as Brucella spp. by PCR and all strains identified asB. melitensis by AMOS- PCR.}, keywords = {Brucella spp,PCR,AMOS-PCR,Sheep and goats}, url = {https://bvmj.journals.ekb.eg/article_35938.html}, eprint = {https://bvmj.journals.ekb.eg/article_35938_b3a9971d20581792877bf8deb2e0c9fa.pdf} } @article { author = {Abd EL-Tawab, Ashraf and Abo El-Roos, Nahla and El-Gendy, A.A.M.}, title = {Effect of Zinc Oxide Nanoparticles on Staphylococcus AureusIsolated From CowsʼMastitic Milk}, journal = {Benha Veterinary Medical Journal}, volume = {35}, number = {1}, pages = {30-41}, year = {2018}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2018.35946}, abstract = {The current spreading of nanomaterial applications supports the search for further possible functions of theses diminutive particles. The antibacterial potentiality of zinc oxide (ZnO) nanoparticles (NPs) againstS. aureus causing mastitis was evaluated using qualitative and quantitative assays. These tests were performed in nutrient broth and nutrient agar following standard methods. In addition, the effect of different concentrations of ZnO nanoparticles on the growth of S. aureus was measured.MIC was determined using six different  concentrations of ZnO nanoparticles including  16,  8,  4,  2,  1  and  0.5 mg/ml.  The  MIC  value S. aureus  was  0.5  mg/ml.  The  results  showed that ZnO  nanoparticles have  antibacterial  inhibition  zone  of 29 mm at  the  concentration  of 10 mg/ml against S. aureus., and the antibacterial  activity of ZnO nanoparticles  increased  with  increasing  powder  concentration in vitro. }, keywords = {ZnO nanoparticle,Staphylococcus aureus,Minimum inhibitory concentration (MIC)}, url = {https://bvmj.journals.ekb.eg/article_35946.html}, eprint = {https://bvmj.journals.ekb.eg/article_35946_9248c51e45427a695fbd59d72ec9f34b.pdf} } @article { author = {Ibrahim, Hemmat and Hassan, Mohamed and Amin, Reham and Shawqy, Nahla and Elkoly, , R.L.}, title = {Effect of Some Essential Oils on the Bacteriological Quality of Some Chicken Meat Products}, journal = {Benha Veterinary Medical Journal}, volume = {35}, number = {1}, pages = {42-49}, year = {2018}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2018.37965}, abstract = {A total of 1500g of the fresh minced chicken fillet was purchased from butcher shops in El Menofiya Governorate and directly transferred to the laboratory under complete aseptic condition. Experimental trials were pointed toward the ability to control the outgrowth of Staph aureus in chicken fillet using Nigella sativa oil (0.1% and 0.5%) and rosemary oil (0.1% and 0.5%). The sample was divided into 5 groups. All groups were injected with Staph. aureus reference strain (ATCC®25923) and the initial load was 2.6 x 107± 1.04 x 107 cfu/g. Control group was inoculated with the tested culture and was stored without treatment with oils. Both oils reduced Staph. aureus levels significantly from zero day till 6th day in the following order: Nigella sativa 0.5% > Nigella sativa 0.1% > Rosemary 0.5% > Rosemary 0.1%. In contrast in the control group,the count increased rapidly till the 4th day of the treatment. Moreover, both oils improved the overall acceptability and prolonged the shelf life of treated samples as it remains without putrefactive changes till 6th day in comparison with control samples which remain without putrefactive changes till 4th day only.   Finally, it can be concluded that essential oils possess significant antibacterial activity against the Staph. aureus and increases with increasing their concentration.}, keywords = {preservatives,Nigella Sativa,rosemary}, url = {https://bvmj.journals.ekb.eg/article_37965.html}, eprint = {https://bvmj.journals.ekb.eg/article_37965_7bcffb71a9d139966e74ab6d349f5dc0.pdf} } @article { author = {Ibrahim, Hemmat and Hassan, Mohamed and Amin, Reham and Shawky, Nahla and Elkoly, R.L.}, title = {The Bacteriological Quality 0f Some Chicken Meat Products}, journal = {Benha Veterinary Medical Journal}, volume = {35}, number = {1}, pages = {50-57}, year = {2018}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2018.37967}, abstract = {The chicken meat is one of the main sources of animal protein all over the world; and the volume of their production, marketing and consumption is increasing to satisfy the public demand worldwide within  the  last  decades (Lyon et al., 2007). A total of 90 random samples of chicken meat products (nuggets, luncheon and frozen shawerma) (25 g, 30 of each), were collected from different supermarkets at El- Menufia governorates. The mean values of aerobic plate, coliforms and Staphylococci counts were 1.99 ×105 ± 0.62×105, 1.14×103 ± 0.35×103 and 4.3×102 ± 1×102 (cfu/g) in chicken luncheon, 3.83 ×105 ± 9.6×104, 5.0×103± 1.2 ×103, 1.6×103± 0.42×103cfu/g, in chicken nuggets and 2.77×106 ± 1.4×106, 6.33×103± 1.7×103and 1.97×103± 0.52×103 (cfu/g) in shawerma, respectively. The incidence of Staphylococcus aureus in the examined samples of chicken luncheon, nuggets, and shawerma wad 7 (23.3%), 9 (30%) and14 (46.6%), While the incidence of E. coli was 4 (13.3%), 5 (16.6%) and 7 (23.3%), in the examined samples of luncheon, nuggets, and shawerma, respectively. Most of the examined chicken meat products were contaminated with E. coli and Staphylococcus aureus and this is considered objectionable.}, keywords = {Chicken Meat Products,APC,E.coli,S.aureus}, url = {https://bvmj.journals.ekb.eg/article_37967.html}, eprint = {https://bvmj.journals.ekb.eg/article_37967_b80d8a9bb7806b327404baac2b039d83.pdf} } @article { author = {hassan, Mohamed and Amin, Reham and El-Taher, O.M, and Meslam, E.M.}, title = {Chemical Preservatives in Some Meat Products}, journal = {Benha Veterinary Medical Journal}, volume = {35}, number = {1}, pages = {58-65}, year = {2018}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2018.38118}, abstract = {A grand total of 120 random samples of meat products represented by beef burger, beef sausage, beef minced meat and beef kofta (30 of each) were collected from different supermarkets located in Cairo and Giza.The average of nitrite, phosphate, ascorbic acid and monosodium glutamate levels (ppm) were 24.14 ± 1.96, zero, 111.33 ± 9.49 and 983.12 ± 45.71in the examined minced meat, respectively,38.53 ± 2.17, 0.13 ± 0.01, 190.10 ± 14.26 and 1849.27 ± 92.69in the examined kofta, respectively,36.89 ± 2.02, 0.21 ± 0.01,227.60 ± 16.81 and 1139.90 ± 73.14in the examined beef burger, respectivelyand 43.59 ± 2.33,0.16 ± 0.01, 265.67 ± 21.04 and 1958.83 ± 101.35in the examined sausage, respectively.}, keywords = {meat products,Nutritional criteria,chemical preservatives,nitrite,Phosphate,ascorbic acid,MSG}, url = {https://bvmj.journals.ekb.eg/article_38118.html}, eprint = {https://bvmj.journals.ekb.eg/article_38118_f66a7b56fb121ecb58272257210e5e6b.pdf} } @article { author = {Hassan, Mohamed and Amin, Reham and El-Taher, O.M. and Meslam, E.M.}, title = {Heavy Metals in some Meat Products and Its Biocontrol by Probiotic Strain Enterococcus Faecium (M74) in Minced Meat}, journal = {Benha Veterinary Medical Journal}, volume = {35}, number = {1}, pages = {66-75}, year = {2018}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2018.38126}, abstract = {This study was conducted to detection of heavy metals and its biocontrol by probiotic strain enterococcus faecium M74.A grand total of 120 random samples of meat products represented by beef burger, beef sausage, beef minced meat and beef kofta (30 of each) were collected from different supermarkets located in Cairo and Giza governorate. The average of lead, cadmium and arsenic levels (ppm) were 0.13 ± 0.01,0.07 ± 0.01 and 0.04 ± 0.01in the examined beef minced meat, respectively,0.24 ± 0.01, 0.11 ± 0.01 and 0.04 ± 0.01 in the examined beef kofta, respectively, 0.21 ± 0.01, 0.18 ± 0.01 and zero in the examined beef burger, respectively and 0.36 ± 0.02, 0.16 ± 0.02 and 0.10 ± 0.02 in the examined beef sausage, respectively. The E. faecium strain (M74) removed lead and cadmium efficiently from the minced meat. Lead content (50 ppm) in the group treated with E. facieum (M74) showed reduction after 8, 16 and 24 hrs of incubation, their reduction rate was 66.4%, 73% and 82.6%, respectively. While, cadmium content (10ppm) in the group treated with E. facieum (M74) showed reduction after 8, 16 and 24 hrs of incubation, their reduction rate was 59%, 67% and 71%, respectively.}, keywords = {Nutritional criteria,preservatives,Heavy metals,Enterococcus faecium M74}, url = {https://bvmj.journals.ekb.eg/article_38126.html}, eprint = {https://bvmj.journals.ekb.eg/article_38126_357da781c53415e683d85979763d1b5e.pdf} } @article { author = {Habak, S. M. and Azab, M. E. and Ismail, R. S. and Nafeaa, A. A.}, title = {Effectof Castration on Some Immunological Aspects in Male Albino Rat}, journal = {Benha Veterinary Medical Journal}, volume = {35}, number = {1}, pages = {76-83}, year = {2018}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2018.38198}, abstract = {The current study aimed to evaluate the effect of surgical and chemical castration on some immunological aspects in adult male albino rats. A total number of forty adult male albino rats weighing 220±20 g and averaging 4 months old were used. Rats were divided into four equal groups. Group 1, intact control group; group 2, sham castrated group; group3, surgically castrated group and group 4, chemically castrated group. Relative thymus and spleen weight, peripheral total and differential leukocytic count and relative expression of TNFα gene were recorded at 2 and 4 weeks after castration. The obtained results revealed that surgical and chemical castration resulted in a significant increase in relative thymus and spleen weight. Peripheral total leukocytic count was significantly increased in surgically and chemically castrated groups as compared to intact control and sham groups. Differential leukocytic count revealed a significant increase in lymphocyte number and a significant decrease in neutrophil number in surgically and chemically castrated groups as compared to intact control and sham groups. Real-time quantitative PCR analysis of TNFα gene expression in spleen revealed that surgical and chemical castration resulted in significant up regulation of TNFα gene expression. So, it concluded that surgical and chemical castration improved immunity and testosterone has an immunosuppressive effect in adult male albino rats.}, keywords = {surgical,chemical,castration,Rats,Immunological aspects}, url = {https://bvmj.journals.ekb.eg/article_38198.html}, eprint = {https://bvmj.journals.ekb.eg/article_38198_62d5867a8501c563e0f2d1d3acdaeadb.pdf} } @article { author = {Abd EL Maksoud, Hussine and Abd El Hamid, omnia and M., Mona and Emara, M.O}, title = {Biochemical Effect of Cholchicine on Experimental Leukemia in Rats}, journal = {Benha Veterinary Medical Journal}, volume = {35}, number = {1}, pages = {84-93}, year = {2018}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2018.38203}, abstract = {Colchicine is an alkaloid that has been widely used for treatment of gout. It also has a curative effect on cancer, as many studies have shown that its effect on cell apoptosis .The objective of the present study was to evaluate the Biochemical Effect of Cholchicine on Experimentally Induced Leukemia in Rats. Sixty white albino male rats of 8-10 weeks old and 150-200 g weight were ued in the experiment.Rats were randomly divided into six groups 1st group act as normal control , 2nd group was injected intraperitoneally with colchicine (0.14mg/kg/wt) one dose daily for four weeks and act as positive control , 3rd group was administrated orally with benzene (1mg /kg /wt) one dose daily four weeks for induction of leukemia and act as leukemic group , last three groups (4th , 5th ,6th ) administrated benzene at a dose of (1mg /kg /wt) daily and treated with colchicine with different concentration (0.07mg/kg/wt)(0.14mg/kg/wt) and (0.21mg/kg/wt) respectively. Blood samples were collected twice after 2 and 4 weeks for biochemical examination. Intraperitoneal injection of colchicine caused increased in Hb level, platelets count and Caspase- 3 and decreased in Total leucocyte count (WBCs),L.malondialdehyde(L-MDA), Super Oxide Dismutase (SOD) ,Catalase (CAT), Glutathione peroxidase (GPx) , Glutathione (GSH),Alpha –fetoprotein (AFP) ,Interleukin-2 (IL-2),Interleukin-6 (IL-6),Tumor necrosis factor (TNFα), compared with diseased group}, keywords = {Leukemia,Benzene,Colchicine,TNFα,IL-6}, url = {https://bvmj.journals.ekb.eg/article_38203.html}, eprint = {https://bvmj.journals.ekb.eg/article_38203_9d73c3c3c609124a06cd32c01061d723.pdf} } @article { author = {Mohi-Eldin, Mouchira M. and Haridy, Mohie A.M. and Hussein Khalil, Atef Mohammed and Abdelnaeim, Karima}, title = {Immunomodulatory and antiparasitic effects of garlic extract loaded on zinc oxide nanoparticles compared with pure garlic extract on Eimeriastiedae-infected rabbits}, journal = {Benha Veterinary Medical Journal}, volume = {35}, number = {1}, pages = {94-105}, year = {2018}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2018.38214}, abstract = {The study aimed toinvestigate the immunomodulatory and antiparasitic effects of garlic extract- loaded zinc oxide nanoparticles in comparison to pure garlic extract against  rabbit hepatic coccidiosis. Sixty male rabbits were allocated into four groups; Group 1: control, Group 2 (infected group): rabbits were inoculated orally with 2 x 103 sporulated oocyst of E. stiedae , Group 3: rabbits were infected as group 2 and treated with garlic extract (500 mg/kg b.w) and Group 4: rabbits infected and treated with garlic extract nanoparticles (500 mg/kg b.w). Five rabbits of each group were scarified at 7th, 14th and 21th day post inoculation (dpi).  Biochemical, haematology and antioxidant parameters were evaluated. Fecal samples were collected at 19th day to calculate the number of oocyst. Results revealed: hemoglobin concentration and PCV significantly increased in group 4 when compared to group 2, and platelets count of group 3 significatly increased when compared with group 2. Significant decreases in ALT, AST, Alkaline. phosphatase, bilirubin were detected in groups 3 and 4 compared with group 2, but significant decreases in MDA and SOD in groups 3 & 4 compared with group 2. Group 2 had significant increases in ALT, AST, Alkaline phosphatase, bilirubin, MDA and SOD activities when compared with control group. Significant increases in AST and ALP activities were detected in group 4 in comparison with group 3. Group 3 had a significant decrease in SOD when compared with group 4. Group 3 and 4 induced significant reduction in fecal oocyst count compared with group 2. Conclusively, garlic crude extract and garlic nanoparticles had antioxidative and anti-parasitic effect on hepatic coccidiosis of rabbit.}, keywords = {Garlic nanoparticle,garlic extract,Eimeriastiedae,antioxidant}, url = {https://bvmj.journals.ekb.eg/article_38214.html}, eprint = {https://bvmj.journals.ekb.eg/article_38214_ba7612dcd57ad1fd325a06a7dd86e7c0.pdf} } @article { author = {M. Ibrahim, Hemmat and El Sabagh, Rasha and A. Wahba, Ahmed and Abd El Rahman, El Sayed A.}, title = {The Incidence of Sarcocystis in Slaughtered Food Animals}, journal = {Benha Veterinary Medical Journal}, volume = {35}, number = {1}, pages = {106-122}, year = {2018}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2018.38219}, abstract = {Sarcocystis affects the meat quality, rendering meat unmarketable and unacceptable to consumers. In the present study, total (2000) Slaughtered animals were examined during P.M inspection then the Macroscopic and Microscopic examinations were done for detection of Sarcocystis followed by Polymerase chain reaction (PCR) in El-Basateen automated abattoir in Cairo, Egypt. With special references to species, age, and sex of slaughtered animals, our study revealed that the higher incidence (macroscopic and microscopic) in old buffaloes (48.6%-63.2%) than young ones (41.2-53.8%), respectively followed by cattle, sheep finally camel. In addition to the higher incidence (macroscopic and microscopic) in female buffaloes (25.8%-36.2%) than male ones (37%-49.6%), respectively followed by cattle, camel finally sheep. The PCR confirmed the presence of Sarcocystis in examined samples. SO that, the work recommended that Using of Microscopic examination and PCR for detection and confirmation of Sarcocystosis in abattoirs to avoid animals and human infections with such zoonotic parasite.}, keywords = {meat,slaughtered food animals,Parasites,PCR}, url = {https://bvmj.journals.ekb.eg/article_38219.html}, eprint = {https://bvmj.journals.ekb.eg/article_38219_c5a69d317a6d89c3075ae900e1c7ae6d.pdf} } @article { author = {AbouZaid, Omayma, A.R. and A.H, Samy, and A.A., Samir, and Z.M., Fatma,}, title = {Biochemical effect of leuprolide, cabergoline and some herbals on ameliorating polycystic ovaries syndrome}, journal = {Benha Veterinary Medical Journal}, volume = {35}, number = {1}, pages = {123-133}, year = {2018}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2018.38231}, abstract = {Polycystic ovary syndrome (PCOS) is a set of symptoms due to elevated androgens\in females. Its Signs include irregular or no menstrual periods, heavy periods, excess body and facial hair, acne, pelvic pain, difficulty getting pregnant, and patches of thick, darker, velvety skin. It could be a result from the combination of genetic and environmental factors. Many drugs used for treating this disease. Several hormones have been measured to evaluate PCOS (progesterone, Estradiol, Follicular Stimulating Hormone (FSH), Luteinizing hormone (LH), Total Testosterone, Anti-Mullerian Hormone (AMH), Thyroid Stimulating Hormone (TSH), Prolactin and histopathological studies. Groups were classified as follow: Group (1) Control, Group (2) Dehydroepiandrosterone (DHEA only), Group (3) (DHEA and flaxseed), Group (4) (DHEA and alfalfa), Group (5) (DHEA and Soya), Group (6) (DHEA and Cabergoline), and Group (7) (DHEA and Leuproline). Results: DHEA in a dose of (1mg/kg) induced PCOS and it was clear by significant elevation of (LH/ FSH ratio, total testosterone, AMH, TSH, Prolactin) and a significant decrease in progesterone and estradiol. Leuproline was the best choice to decrease the symptoms of PCOS followed by cabergoline and the least but still effective were the herbals (Soya, flaxseed, and alfalfa).  In conclusion, DHEA could be used for PCOS induction, Leuproline, cabergoline and phytoestrogen containing herbals could be used for treating PCOS symptoms.}, keywords = {PCOs,DHEA,Leuproline,Cabergoline,Soya,alfalfa,Flaxseed}, url = {https://bvmj.journals.ekb.eg/article_38231.html}, eprint = {https://bvmj.journals.ekb.eg/article_38231_3ccb18ecd377528c40cb9f0992d54a0f.pdf} } @article { author = {B.A, Ahmed, and G.F, El-Bagoury, and A.S., El-Habbaa and M.H., Khodeir,}, title = {Experimental infection of goslings with Duck Hepatitis Virus type-1}, journal = {Benha Veterinary Medical Journal}, volume = {35}, number = {1}, pages = {134-144}, year = {2018}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2018.38251}, abstract = {The present work investigated the susceptibility of goslings to infection with Duck Hepatitis Virus type-1 (DHV-1) and their possible role for hazard spread of infection to ducklings. It was noticed that experimentally infected one-week old goslings were susceptible to infection with DHV-1 showing clinical signs started by the 4th day post infection detected in 4 out 10 goslings represented by dullness and weakness. Two goslings were recovered, and other 2 birds were died one-week post infection. In contact ducklings showed classical signs of DHV-1 infection represented by weakness and sudden death. DHV-1 was recovered from liver, spleen and kidneys of experimentally dead birds. Survived goslings exhibited detectable neutralizing antibody titers by the 1st week post infection. Vaccination of goslings with the live attenuated DHV-1 vaccine resulted in good immune response of high antibody levels up to 10 weeks post vaccination. So, it could be said that goslings were susceptible to DHV-1 and may play a role in transmission of DHV-1 to duckling and it could be recommended to vaccinate gosling against DHV-1 infection}, keywords = {Duck hepatitis virus type- 1 (DHV-1),gosling,duckling}, url = {https://bvmj.journals.ekb.eg/article_38251.html}, eprint = {https://bvmj.journals.ekb.eg/article_38251_92e1d03727c802e29492ea5ffe72d7f4.pdf} } @article { author = {B.A., Ahmed, and G.F., El-Bagoury, and A.s., El-Habbaa, and M.H., Khodeir,}, title = {The possibility of Duck Hepatitis Virus type 1 (DHV-1) transmission from experimentally infected ducklings to goslings}, journal = {Benha Veterinary Medical Journal}, volume = {35}, number = {1}, pages = {145-151}, year = {2018}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2018.38259}, abstract = {The present work aimed to investigate the possibility of DHV-1 transmission from infected ducklings to goslings and to spot the light on gosling susceptibility to such virus infection where they may play a neglected role in the disease outbreaks. The work included experimental infection of a group of one-week old duckling with the virulent Duck Hepatitis Virus – 1 (DHV-1) that was housed in contact with one-week old gosling group. It was found that 2 out of 10 in contact goslings exhibited clinical signs of DHV-1 infection by the 5th day post housing with infected ducklings showing dullness and weakness then recovered within one week. Survived goslings exhibited detectable serum neutralizing antibody titers by the 1st week post infection. These findings indicate that gosling can attract DHV-1 infection from infected ducklings. }, keywords = {Duck hepatitis virus type- 1 (DHV-1),gosling,duckling}, url = {https://bvmj.journals.ekb.eg/article_38259.html}, eprint = {https://bvmj.journals.ekb.eg/article_38259_02a70a554bd1507932bf8d0477223f0b.pdf} } @article { author = {H.A., Abdelmaksoud and M. Abdelhamid, Omnia, and E.A. Ismaeel, Taheya, and Elkharadly, Waheed A.}, title = {Biochemical effects of Pomegranate Grinded Peels on High Sucrose Stressed Rabbits}, journal = {Benha Veterinary Medical Journal}, volume = {35}, number = {1}, pages = {152-163}, year = {2018}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2018.38265}, abstract = {Pomegranate is a drought tolerant and long lived plant which grows popularly in arid and semiarid zones in the Mediterranean countries. Pomegranate was reported to have antimicrobial, anti-inflammatory, anti-tumor, anti-hepatotoxic, antiviral, anti-diabetic activities and they can improve oral, skin and cardiovascular health. Sucrose is a non-reducing disaccharide made up of 50% glucose and 50% fructose and has a moderately high glycemic index. High-sucrose diets have been used in animal models to induce well-characterized metabolic dysfunctions including cardiovascular risk factors, insulin resistance, dyslipidemia and hypertension. The present research aimed to evaluate the effects of pomegranate grinded peel extract supplementation on blood parameters of male rabbits stressed by high sucrose diet for 4 months, Through evaluation of serum total lipids, triacylglycerols, total cholesterol, HDL-c, LDL-c, VLDL-c and atherogenic indices, in addition to serum urea, creatinine, glucose, testosterone, zinc and copper concentrations. High sucrose supplementation induced a significant increase in Serum total lipids, triacylglycerols, total cholesterol, LDL-c, VLDL-c, atherogenic indices, glucose, testosterone and copper concentrations, in addition to non-significant increase in serum urea, creatinine concentrations. On contrast, it exhibited a significant decrease in serum HDL-C and zinc concentrations. Pomegranate peel powder supplementation was able to mitigate and ameliorate stress induced by high sucrose ration and showed pronounced curative effect against hyperlipidemia and deviated serum atherogenic indices as well as maintained glucose, testosterone copper and zinc toward control levels. The results of the present study suggested that pomegranate has the potential to exert curative effects against hyperlipidemia.}, keywords = {pomegranate,sucrose%,hyperlipidemia,Oxidative Stress}, url = {https://bvmj.journals.ekb.eg/article_38265.html}, eprint = {https://bvmj.journals.ekb.eg/article_38265_3004fe6e44a20eba25a8ee99486beb06.pdf} } @article { author = {H.A., Abdel maksoud and M. A., Omnia and M., El-Bialy}, title = {Biochemical Study of Anti-Inflammatory Effect of Osteoblastic Activator in Experimental Pancreatic Degeneration in Rat}, journal = {Benha Veterinary Medical Journal}, volume = {35}, number = {1}, pages = {164-170}, year = {2018}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2018.38349}, abstract = {A total number of 160 Wister female rats, 12 -wk. old were , weighed and divided into five experimental groups to investigate the effect of streptozotocin (STZ) injection (30 and 60 mg/kg B.W) with and without zoledronic acid (0.15 mg/kg B.W) on some blood serum constituents such as  interleukins (IL-2, IL-6), immunoglobulin E (IgE) and cortisol. The results indicated that it's paralleled to the published data on the hyperglycemia and inflammatory condition in experimentally pancreatic degeneration. The current results showed that, injection with STZ was associated with a statistically significant elevation in serum IL-2, IL-6, IgE and cortisol but zoledronic treatment with STZ resulted in a decrease in serum IL-2, IL-6, IgE and cortisol. These results indicated that ZOL acid has beneficial effect to slow pancreatic carcinoma induction when treated with STZ at once in experimental animals}, keywords = {STZ,Zoledronic acid,pancreas}, url = {https://bvmj.journals.ekb.eg/article_38349.html}, eprint = {https://bvmj.journals.ekb.eg/article_38349_0ea08d696130bf448d9347682e6a5097.pdf} } @article { author = {H.A, Abdel Maksoud and M. A., Omnia and M., El-Bialy}, title = {Biochemical Effect of Zolderonic Acid on Experimentally Induced Degeneration of Pancreas}, journal = {Benha Veterinary Medical Journal}, volume = {35}, number = {1}, pages = {171-177}, year = {2018}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2018.38352}, abstract = {A total number of 160 Wister female rats, 12 -wk. old were taken, weighed and divided into five experimental groups to investigate the effect of streptozotocin (STZ) injection (30 and 60 mg/kg B.W) with and without zoledronic acid (0.15 mg/kg B.W) on some blood serum constituents such as glucose, insulin, calcium, phosphorus and vitamin D. The current results showed that, injection with STZ was associated with a statistically significant elevated in serum glucose, while there was a decrease in serum insulin, calcium and phosphorus. However, zoledronic treatment resulted in a decrease in serum glucose, mean - while it elevate serum insulin, calcium and phosphorus. toward normal Level These results indicated that ZOL acid has beneficial effect on pancreatic biochemical parameters in case of degenerated pancreas.}, keywords = {STZ,Zoledronic acid,pancreas}, url = {https://bvmj.journals.ekb.eg/article_38352.html}, eprint = {https://bvmj.journals.ekb.eg/article_38352_a64299928128642738ea6a2da2e169b3.pdf} } @article { author = {Abouzaid, OmaymaA.R and Mansour, Somaya Z. and Sabbah, Fatma}, title = {Evaluation of the antitumor activity of Solenostemma argel in the treatment of lung carcinoma induced in rats}, journal = {Benha Veterinary Medical Journal}, volume = {35}, number = {1}, pages = {178-189}, year = {2018}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2018.38357}, abstract = {The present study was designed to evaluate the suspected anticancer activity of Solenostemma argel (200 mg/kg body weight/ day) either alone or combined with cisplatin (0.64 mg/kg body weight/day) for 4 weeks against lung cancer induced by DMBA (20 mg/kg bw) for 8 weeks in male Wistar rats.At the end of experiment, blood and Lung samples were collected to assess biochemical and biophysical markers as well histopathological investigations. Biochemical parameters:, serum Tumor suppressor protein (P53) and (P16), Transforming Growth Factor-beta (TGF-β), interleukin-1beta (IL-1β), interleukin-6 (IL-6), Carcino Embryonic antigen (CEA). Lung induced nitric oxide synthase (i-Nos), cyclin dependent kinase-2 (CDK2). The level of significance between mean values was set at p < 0.05. The results of this study also showed that administration of olenostemma argel to lung carcinoma induced rats demonstrated a significant improvement in biochemical parameters and life span as compared to DMBA control rats. The histopathological examinations of this study revealed damage and degeneration in the lung of DMBA treated rats.  Also, lung of Argel and Cisplatin rats showed significant improvement and protection against DMBA harmful effect.}, keywords = {Cisplatin,Solenostemma argel,7.12- dimethylbenz (α) anthracene}, url = {https://bvmj.journals.ekb.eg/article_38357.html}, eprint = {https://bvmj.journals.ekb.eg/article_38357_d6986c3f9bc402f8e26b9db9f98be5ac.pdf} } @article { author = {EL-Maghraby, Marwa Salah and Hassan, Mohamed and Hassanin, Faten and Shawky, Nahla}, title = {Detection of EnterotoxigenicStaphylococcus aureusin Meat Product Sandwiches Using Multiplex PCR}, journal = {Benha Veterinary Medical Journal}, volume = {35}, number = {1}, pages = {190-196}, year = {2018}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2018.38362}, abstract = {The current work was performed to detect Staphylococcus aureus and their enterotoxin genes as sea, seb, sec andsed among street vended meat products represented by beef burger, kofta and sausage (30 of each) using traditional method of bacteriological isolation and multiplex PCR. The results illustrated that 37 (41.11%) out of 90 meat product samples harbored S.aureuspathogen. Accurately, S.aureus enterotoxin genes were detected in 13 (35.1%) out of 37 isolates. 3 isolate for beef burger samples mostly harboured (2) sea and (1) seb and the other 6 strains was negative for classic enterotoxin genes. Meanwhile, 5 isolates for kofta samples harboured (2) sea, (1) sec, (1)sedand(1) sea&sedgene and the other 7 were negative for classic enterotoxin genes, but 16 isolates of sausage samples harboured (2) sea, (1) seb, (1) mixed seb&sec and (1) mixed sea, sec & sec genes and 11 was negative for classic enterotoxin genes. Furthermore, seais more prevalent in 6 samples (16.2%) followed by sebin 2 samples (5.4%) and sec, sed, sea &sed, seb&secand sea &sec&sed in one sample (2.7%). Finally, Multiplex PCR is efficient in detection of SEs in food. Contamination of meat products with such serous pathogen was discussed.}, keywords = {S. aureus,Multiplex PCR}, url = {https://bvmj.journals.ekb.eg/article_38362.html}, eprint = {https://bvmj.journals.ekb.eg/article_38362_1cfe8311dedceaa598fe9ec2d8d1ca1a.pdf} } @article { author = {EL-Maghraby, Marwa and Hassan, Mohamed and Hassanin, Faten and Shawky, Nahla}, title = {Street Vended Meat Products as Potential Sources of Staphylococcus aureus}, journal = {Benha Veterinary Medical Journal}, volume = {35}, number = {1}, pages = {197-202}, year = {2018}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2018.38364}, abstract = {A total of 90 random samples of street vended meat products represented by beef burger, kofta and sausage (30 of each) were collected from several street vendors in Menofyia government. for isolation and identification of S. aureus as well as detection of Staphylococcal enterotoxins. The obtained results indicated that the mean values of Staphylococci count (cfu/g) in the examined samples were 4.27×103 ± 0.69×103 for beef burger, 9.15×103 ± 2.01×103for kofta and 1.62×104 ± 0.37×104 for sausage. The results obtained indicated that 16 (53.33%) samples of beef burger,19 (63.33%) samples of kofta and 21(70%) samples of sausage were unaccepted according to their Staphylococci counts/g according to Egyptian Organization for Standardization "EOS" (2005). The incidence of S. aureus in the examined samples of sausage was 53.33% followed by kofta samples (40%). While, the lowest incidence of S.aureus was recorded in the examined beef burger samples(30%). The study obtained "one" enterotoxin A, enterotoxin C and enterotoxin A+D produced by S. aureus in the sausage samples of meat product. While, enterotoxins B and enterotoxin D failed to be detected in sausage samples of meat product. concerningkofta samples, 2 enterotoxin D produced by S. aureus. While, enterotoxins A, B and C were not detected in kofta and beef burger samples. Finally, the probable sources of contamination of meat products with such serous pathogen and some recommendation to control its enterotoxins production were discussed.}, keywords = {S. aureus,Incidence,Enterotoxins}, url = {https://bvmj.journals.ekb.eg/article_38364.html}, eprint = {https://bvmj.journals.ekb.eg/article_38364_5137a29483c9bdb3cfc9bbd14084be0e.pdf} } @article { author = {El-Sayed, Mossad and El-Komy, Ashraf and Salah, Eman}, title = {The Pharmacodynamic Profile of Hydroethanolic Seed Extract of Moringa Oleifera on Intestinal and Uterine Contractility}, journal = {Benha Veterinary Medical Journal}, volume = {35}, number = {1}, pages = {203-215}, year = {2018}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2018.38367}, abstract = {Moringa oleifera is extensively distributed and used in many countries all over the world. The seed of Moringa oleifera is a high value part and has an amazing medicinal and therapeutic uses with high nutritional value. This study, therefore, was designed to clarify the pharmacological effects of hydroethanolic seed extract of Moringa oleifera(HSMO) on isolated intestinal and uterine motility with a view to finding out the site of actions of Moringa oleifera (MO). Tension recording techniquewas used for studying the effect of Moringa oleifera seedsextract on isolated rabbit's duodenum, guinea pig's ileum, rat's colon and uterus. Results obtained showed that HSMOproduced a concentrations dependent relaxation of intestinal muscle. The minimal effect of MO was observed at concentration of 4 μg/ml bath that produced 6.3% inhibition of duodenum contractility. Complete relaxation of duodenum smooth muscle was established after the addition of MO at concentration of 512 μg/ml bath. While in isolated guinea pig's ileum and rat's colon, complete inhibition was established after the addition of MO at concentration of 256 μg/ml bath. HSMO inhibited the contractility of rat's uterus during nonpregnant and pregnant stages. Complete relaxationwas attained by addition of 256 µg of MO extract/ml bath. The inhibitory action of MO could be attributed to, at least partly, its action on muscarinic receptors, indicated by decreasing stimulant effect of Ach. The phytochemical screening of Moringa oleifera seeds in our investigation indicated the presence of flavonoids and tannins and these components play a key role in spasmolytic activity by blocking muscarinic receptors. It could be concluded that, MO may have a depressant effect on intestinal and uterine muscles via muscarinic cholinergic pathway. These data may support the effective and safe use of MO in gastrointestinal disorders and pregnancy and these finding may give evidence to its traditional use on the management of intestinal ailments such as pains, diarrhea and dysentery.}, keywords = {Moringa oleifera,Smooth muscle,Spasmolytic activity,Autonomic pharmacology}, url = {https://bvmj.journals.ekb.eg/article_38367.html}, eprint = {https://bvmj.journals.ekb.eg/article_38367_bca654e99df957b2fc5e8fb2c1dca3c4.pdf} } @article { author = {Abd El-Hamid, Omnia, and El-Shaboury, Ayman and Baraka, Ashraf, and Khater, Hanem,}, title = {Biochemical and Histopahthological Changes on Toxoplasma Infected Rats Treated with Lincocin and/ or Green Tea}, journal = {Benha Veterinary Medical Journal}, volume = {35}, number = {1}, pages = {216-227}, year = {2018}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2018.38368}, abstract = {Toxoplasma gondii is a blood parasite of felids with a wide range of intermediate hosts including humans. The present study was carried out to evaluate the efficacy of acute toxoplasmosis on liver, kidney functions and fertility hormones of infected rats, also the effect of Lincocin and green tea oil and their combination on improving the side effects of acute infection.  A total number of 40 pathogen and Toxoplasma-free Sprague-Dawley rats were divided into four groups as follows: Group (1) non infectednon treated; Group (2) infected non treated, Group (3) infected and treated with Lincocin; Group (4) and infected and treated Lincocin and the addition of green tea. The recorded data revealed a significant elevation in levels of ALT, AST, T. Bilirubin, urea and creatinine and a significant decrease of the levels of albumin, progesterone, and testosterone in infected non- treated rats when compared with those of the non- infected group, while cortisol level was not affected.  It could be concluded that T. gondii could affect liver and kidney functions and adversely affect major reproductive hormones. Therefore, it is recommended for the In Vitro Fertilization (IVF) and fertility centers to investigate T. gondii infection in the serum of infertile men as a routine test and treat the positive cases. Treatments with Lincocin plus green tea could be the drug of choice to avoid side effects of acute toxoplasmosis.}, keywords = {Toxoplasmosis,fertility Hormones,Liver and kidney functions,Lincocin,green tea}, url = {https://bvmj.journals.ekb.eg/article_38368.html}, eprint = {https://bvmj.journals.ekb.eg/article_38368_76f52cddebf0a7004a3141aef3c39b94.pdf} } @article { author = {Saad, Saad and Abouel-Roos, Nahla and El-Shazly, Enas}, title = {Food Poisoning Bacteria in Meat-Based Sandwiches}, journal = {Benha Veterinary Medical Journal}, volume = {35}, number = {1}, pages = {228-235}, year = {2018}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2018.38375}, abstract = {A total of 80 random samples of fast foodsrepresented by beef kofta, beef burger, beef shawerma, hawawshi (20 of each) were collected from different fast food restaurants in different districts at Monufia governorate, Egypt. All the collected samples were subjected to determine their quality. The obtained results indicated bacteriological examination and serotyping of Enteropathogenic E. coli isolated from the examined samples of ready-to-eat meat sandwiches. it is obvious that the incidence and serotyping of Enteropathogenic isolated from Salmonella the examined samples of ready-to-eat meat sandwiches Staph. Strains were isolated from the examined samples of ready-to-eat meat products. The obtained results in the present study indicated that meat-based sandwiches were contaminated with different types of microorganisms due to many causes mainly bad hygiene and post cooking contamination.}, keywords = {Fast food,Bacteriological Quality,Meat sandwiches}, url = {https://bvmj.journals.ekb.eg/article_38375.html}, eprint = {https://bvmj.journals.ekb.eg/article_38375_cac1d6917945c7f170f23a117a881d6e.pdf} } @article { author = {A. Abdel-aziz, Elsayed and M., Sawsan and Emam, El-Shaikh, and E., E. and ., Nasralla and E, A.}, title = {Concurrent Use of Tilmicosine and Thyme Oil in Treatment of Mycoplasma gallisepticum in Chickens}, journal = {Benha Veterinary Medical Journal}, volume = {35}, number = {1}, pages = {236-249}, year = {2018}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2018.38685}, abstract = {The objective of this study is designed to clarify the effect of tilmicosin and thyme oil on treatment of chronic respiratory disease in broiler chickens. Eighty one-day old chicks tested negative Mycoplasma gallisepticum (MG). On the 22th day of age the chicks were divided into five equal groups Gp. (1) non infected non treated ( control ),  but chicks in Gp. (2, 3, 4, and 5) at22th day of age were inoculated with 0.2ml of MG broth into air sac. Gp (1) non infected non treated ( control), Gp.(2) infected non treated, Gp.(3)infected treated with thyme oil 200 ppm for 5 successive days, Gp.(4) infected treated with tilmicosin 25 mg⁄kg for 5 successive days. andGp.(5) infected treated with both thyme oil and tilmicosin in same dose and period. At 1st and 7th day post treatment all chicks in all groups were weighed and blood samples were collected for hematobiochemical studies, biochemical studies and phagocytic activity. The clinical signs observed ininfected broilers chickens were poor appetite, depression, nasal discharge, mild conjunctivitis, sneezing, coughing, gasping, rales and mortalities were 20%.  Our results revealed that infected non treated chicks showed significant decrease in body weight gain, RBCs, Hb, PCV, WBCs, T.Protein, albumin and globuln beside significant increase in AST, ALT, creatinine, uric acid, phagocytic activity and phagocytic index at 1st day post treatment. Treated infected broilers chickens with tilmicosin and thyme oil showed improvements in clinical signs, mortalities and body weight beside improvement in hematobiochemical and biochemical parameters and phagocytic activity post treatment. The pathological examination of internal organs of MG infected broiler chickens showed severe pathological lesions in lung, liver, kidney and heart. But infected broiler chickens treated with both thyme oil and tilmicosin is induced marked improvement in the pathological changes.  It could be concluded that the concurrent use of both thyme oil and tilmicosin is a useful mean to treat and control CRD in broiler chicks.  Thyme oil can be used as an assistant factor to retard CRD in broiler chicks due to its immune enhancer and growth promoter properties.}, keywords = {Tilmicosine and Thyme Oil,Mycoplasma Gallisepticum,chickens}, url = {https://bvmj.journals.ekb.eg/article_38685.html}, eprint = {https://bvmj.journals.ekb.eg/article_38685_16c2356bf4af52e7ea8b3b28b9c364be.pdf} } @article { author = {K.M., Fararah and M.A., Elshemy and A.S. A, Abdalgalil, and A.H., bKhalil, and A.E., Elsaber,}, title = {Clinicopathological and Ultrasonographic Changes during Experimentally Induced Arthritis in Donkey}, journal = {Benha Veterinary Medical Journal}, volume = {35}, number = {1}, pages = {250-262}, year = {2018}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2018.38687}, abstract = {This study was intended to verify the clinicopathological effect of intra-articular (IA) staph aureus and amphotericin B injection in the carpal joints of donkeys to induce arthritic changes and to correlate it with the histopathological findings. Six healthy, adult donkeys of both sexes. Animals were numbered and divided into two groups; each group consists of three animals. 1st group is used for induction of septic carpitis in the right radiocarpal joints of donkeys by Staph. aureus with a dose of 3×106cfu as a single intra-articular injection.  The 2nd group is used for induction of osteoarthritis in the radiocarpal joints of donkeys by 20 mg amphotericin B in 3 ml sterile water as a single intra-articular injection. Whereas the left joint was the control in both groups .Two ml Synovial fluid and serum were taken at 0, 1, 3,7,13 days after administration and tissues samples of Joint capsule and synovial membrane at 13 days. Sampling at zero daywas considered as control.  Clinical assessment, synovial fluid analysis,ultrasonography and histopathological examinations were done. Total leukocytic count, total protein Amyloid A, IL-1β and CRP concentrations sharply increased on 1, 3, 7, 13days 7 after induction of the experiment when compared with their control at zero. .There were significant changes in amyloid A, IL-1β and CRP in septic cases when compared with aseptic cases of arthritis. The results obtained confirm that the concentrations of total leukocytic count, total protein amyloid A, IL-1β and CRP increase substantially in equine septic arthritis and osteoarthritis which provides a theoretical basis for the rapid diagnosis of the diseases and to distinguish between septic arthritis and non-septic synovitis by a variety of diagnostics.}, keywords = {equine,Staph aureus,Ultrasonography,osteoarthritis,Amyloid A,synovial fluid}, url = {https://bvmj.journals.ekb.eg/article_38687.html}, eprint = {https://bvmj.journals.ekb.eg/article_38687_03cefee936f0526c80369cad44ffe762.pdf} } @article { author = {A. Gharib, Ahlam and K. Abd El-Aziz, Nourhan and Hussein, Ashraf and Mohammed, Eman A. A.}, title = {Identification of Pyogenic Bacteria in Human and Animals Conventionally and Non-nucleic Acid Molecularly Using MALDI-TOF MS}, journal = {Benha Veterinary Medical Journal}, volume = {35}, number = {1}, pages = {263-273}, year = {2018}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2018.38692}, abstract = {Sepsis is considered a race to death between pathogens and host immune system, associated with a high mortality worldwide. Until now, blood cultures (BCs) are still the “gold standard” for identification of pathogens causing sepsis. Its major drawback is long turn around time (TAT) of 24–48 h up to several days, which is not compatible with the need for early sepsis diagnosis. Molecular assays as matrix-assisted laser- desorption ionization time-of-flight mass spectrometry(MALDI-TOF MS) reduce the TAT. Herein, the main aim of the study is to develop rapid and highly sensitive molecular proteomic non-nucleic acid assay for identification (ID) of the most common bacteria causing sepsis and pyogenic infections in human and animals. Eighty clinical samples, including 50 animal pus samples and 30 neonatal whole blood samples were subjected for conventional and molecular identification using VITEK-MS. About 70 % of samples were BC positive, including 33/50(66 %) of animal pus samples and 23/30 (76.7 %) of neonatal blood samples. The identified bacterial species were only three conventionally versus twelve using VITEK-MS, with variable confidence value (C.V) that was 99.9 % for 55/58 (94.8 %) isolates, 50 % for 2/58 (3.4 %) isolates and 26.5 % with 1/58 (1.74 %) isolate. The species-level identification for Streptococci was more challenging than for Staphylococci and Enterococci with only 50 % C.V for S. dysagalactiaesubsp.dysagalactiae. Culture provided concordant species- and genus-level identification with VITEK-MS for 24/58(41.3 %) and 34/58(58.6 %) of isolates, respectively. The overall concordance was 100 % for all isolates except P. aeruginosawas 87.5 %.}, keywords = {VITEK-MS,Sepsis,pyogenic bacteria}, url = {https://bvmj.journals.ekb.eg/article_38692.html}, eprint = {https://bvmj.journals.ekb.eg/article_38692_e512b4f648c98837431bdf79e292c098.pdf} } @article { author = {Mady, E.A. and Karousa, M.M and El-laithy, S.M and Ahmed, Souad. A.}, title = {Effect of Season on New Zealand White (NZW) Rabbits' Behaviorand Reproductive and productivePerformance}, journal = {Benha Veterinary Medical Journal}, volume = {35}, number = {1}, pages = {274-284}, year = {2018}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2018.38761}, abstract = {This work was carried out at the experimental animal center belonging to Faculty of Veterinary Medicine, Benha University through the period from July 2017 to the end of February 2018 to study the effect of season of year on behavior, reproductive and productive performance of breeding rabbits using 24 New Zealand White rabbits (18 females & 6 males) with age of 7 months. These animals were randomly assigned to two equal groups. One of these groups was reared during summer season under hot ambient temperature ranged from 28.7° C to 31.8° C and relative humidity ranged from 44.1 to 65.7 %, while the other group was reared during winter season under temperature ranged from 13° C to 20.4° C and relative humidity ranged from 51.55 to 63.3 %. All rabbits were reared under the same managerial conditions. Does were mated with mature bucks. Rabbits under heat stress during summer season showed low frequency of feeding, grooming and investigatory behaviors and low birth & weaning weight of kids. While there was no effect of the season on gestation length, litter size at birth ,conception rate, kindling rate or mortality rate. from this work, we can conclude that the behavior, reproductive and productive performance were negatively affected during summer season than during winter season.}, keywords = {season,Rabbits,Behavior,reproductive,productive performance}, url = {https://bvmj.journals.ekb.eg/article_38761.html}, eprint = {https://bvmj.journals.ekb.eg/article_38761_33d1654eba610b12c1ab546e8c51fb5e.pdf} } @article { author = {A. Abd El-Tawab, Ashraf and A.Saleh, Osama and M. Awad, Somayah and M.Absi, Heba}, title = {Phenotypic and Genotypic Characters onAeromonas Species Causing Mortality in Fishes}, journal = {Benha Veterinary Medical Journal}, volume = {35}, number = {1}, pages = {285-300}, year = {2018}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2018.38765}, abstract = {This study was carried out to identify aeromonas species causing mortalities in some fishes. Atotal number of 102 Nile tilapia fish( Oreochromisniloticus) and 45 common carp (Cyprinuscarpio) all had clinical signs and collected from El-Abbassa fish farms and subjected to full clinical, post mortem and bacteriological examinations.The present study revealed,The most prevailing bacterial diseases affecting Nile tilapia and Common carp were motile Aeromonas(A.sobria, A.veronei, A.jandae, A.hydrophilaandA.cavae) The pathogenicity test revealed that mortality rate was 20 – 100 % in Nile tilapia fish due to A.sobria experimental infection. Some isolates of A.veronei and A.hydrophila were pathogenic to Nile tilapia.Aerolysin and Ahcytoen genes were detected in all isolates of A.sobria subjected to PCR.Resistance genes (qnrs and blaTEM) were also detected in all isolates of A.sobria subjected to PCR. While ermBwas negative for all isolates. A.sobria was sensitive to ciprofloxacin, norfloxacin and trimethoprim and resistant to amoxicillin, erythromycin, gentamycin, rifamycin, bacitracin and cephradine}, keywords = {fish,Aeromonassobria,virulence gene,resistant gene,PCR}, url = {https://bvmj.journals.ekb.eg/article_38765.html}, eprint = {https://bvmj.journals.ekb.eg/article_38765_aa9a11ed26293e9d450750f0c53630e5.pdf} } @article { author = {Saad, Saad M. and F. Khater, Dalia and Zaki, Sara M.}, title = {Food Poisoning Bacteria in Ready to Eat Meat and Chicken Meat Products}, journal = {Benha Veterinary Medical Journal}, volume = {35}, number = {1}, pages = {301-310}, year = {2018}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2018.38771}, abstract = {Contamination of ready to eat foods sold by street vendors,restaurants and markets premises rendering has become a global health problem.The present study was designed to examinebacteriologically some ready to eat products(beef luncheon, chicken luncheon,grilled kofta and grilled chicken)for (E.coli, S.aureus, Sallmonellae and B.cereus.) The incidence of E.coli was high in beef luncheon and chicken luncheon by 24%, S.aureus was high in grilled chicken by 36% and B.cereus was high in grilled kofta by 24%.Salmonellaspp were not be detected in all examined samples.The presenceof these microorganisms in ready to eat food samples makesthem unfit for human consumption. Generally, the application and implementation of Hazard Analysis and Critical Control Point (HACCP) System as a hazards control system must be done in meat serving establishments.}, keywords = {Ready to eat food,B.cereus,Salmonella spp,S.aureus,E.coli}, url = {https://bvmj.journals.ekb.eg/article_38771.html}, eprint = {https://bvmj.journals.ekb.eg/article_38771_c981e9a8569f91300872b1da1689d667.pdf} } @article { author = {Abdel- Maksoud, H.A and Mahfouz, M. K. and Soliman, M.I. and Abbass, M. and El-Badry, M.A.}, title = {Biochemical Role of Pyrethroids on Male Fertility and Seminal Profile of Rat}, journal = {Benha Veterinary Medical Journal}, volume = {35}, number = {1}, pages = {311-318}, year = {2018}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2018.39583}, abstract = {A total number of 80 Wister male rats, 9 -wk. old were taken, weighed and divided into four experimental groups to investigate the effect of Deltamethrin (DLM) oral administration (0.87, 8.7 and 17.4 mg/kg B.W) for a period of 9 weeks on reproductive organs and fertility indices of Wister male rats. The results indicated that DLM caused a significant reduction in Testis weights, sperm count, motility and viability and fructose in semen. Also induced decreases in serum FSH, LH and Estradiol levels however increase in sperm abnormalities. These results indicated that DLM is a toxic pyrethroid pesticide that produced significant reproductive toxicity in treated male rats as revealed by the severely affected parameters}, keywords = {DLM,reproductive toxicity,Sperm quality}, url = {https://bvmj.journals.ekb.eg/article_39583.html}, eprint = {https://bvmj.journals.ekb.eg/article_39583_b69e1e5865a35a607a38ad2970b43bf3.pdf} } @article { author = {Abdel-Maksoud, H.A. and Mahfouz, M. K. and Soliman, M.I and Abbass, M. and El-Badry, M.A.}, title = {Biochemical Effects of Deltamethrin on Inflammatory Markers and Iron Homeostasis}, journal = {Benha Veterinary Medical Journal}, volume = {35}, number = {1}, pages = {319-326}, year = {2018}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2018.39584}, abstract = {      A total number of 80 Wister male rats, 9 -wk. old were taken, weighed and divided into four experimental groups to evaluate the oral toxicity induced by Deltamethrin (DLM) administration (0.87, 8.7 and 17.4 mg/kg B.W) for a period of 9 weeks on Iron homeostasis and inflammatory markers of Wister male rats. The results indicated that DLM was associated with a statistically significant elevated in levels of Interluken2(IL2), Interluken6(IL6),  Histamine, cortisol, Ammonia, Haptoglobulin, Transferrin and total iron binding capacity (TIBC), while decreased in Iron (Fe) and Ferritin levels was recorded. These results indicated that DLM is a toxic pyrethroid pesticide that produced significant Alteration in Iron Homeostasis and inflammatory markers.}, keywords = {DLM,Iron Homeostasis,Inflammation}, url = {https://bvmj.journals.ekb.eg/article_39584.html}, eprint = {https://bvmj.journals.ekb.eg/article_39584_9a64f1d6542f75a95bd1f841a156b4e6.pdf} } @article { author = {El-Barbary, Shaimaa I. and E. Azab, Mohamed and S. Ismail, Randa and A. Nafeaa, Abeer}, title = {Ameliorative Effects of Curcumin and Ginger on Hyperlipidemia – Induced Osmotic Fragility and Phospholipids Composition Changes of Erythrocytes in Albino Rats}, journal = {Benha Veterinary Medical Journal}, volume = {35}, number = {1}, pages = {327-341}, year = {2018}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2018.39641}, abstract = {The present study was designed to investigate the erythrocytic changes caused by hyperlipidemia in rats and to evaluate the possible protective effects of curcumin and ginger supplementation. Forty male albino rats were assigned into four equal groups. Group I (control group), rats were fed basal diet. Group II (high-fat diet, HFD group), rats were fed HFD. Group III (curcumin supplemented HFD group), rats were fed HFD contain 0.2% curcumin. Group IV (ginger supplemented HFD group), rats were fed HFD contain 4% ginger. After 2 months, blood samples were collected from retroorbital venous plexus for determination of osmotic fragility of erythrocytes, membrane phospholipids composition and serum lipid profiles. The obtained results revealed that feeding HFD to rats significantly increased serum TC, TAG, LDL-C and phospholipid (phosphatidyl choline, phosphatidyl serine, phosphatidyl ethanolamine and phosphatidyl inositol) concentrations and significantly decreased hemolysis % at 0.4 and 0.5 % Na Cl concentrations as compared with control group. Whereas these changes ameliorated by dietary supplementation of curcumin and ginger as feeding HFD supplemented with 0.2% curcumin or 4% ginger significantly decreased TC, TAG, LDL-C and phospholipid concentrations and significantly increased hemolysis % at 0.4 and 0.5 % Na Cl concentrations as compared with high fat diet fed group. We concluded that supplementation with ginger and curcumin to HFD rats resulted in a significant decrease in serum lipid concentration, lowering membrane phospholipid and beneficially decreased mean cell fragility of erythrocytes thus restored the normality of erythrocyte fluidity in high fat diet fed rats}, keywords = {hyperlipidemia,erythrocyte osmotic fragility,membrane phospholipids,curcumin and ginger}, url = {https://bvmj.journals.ekb.eg/article_39641.html}, eprint = {https://bvmj.journals.ekb.eg/article_39641_97b2718afe194a893ff16d2e9b768386.pdf} } @article { author = {A. R. AbouZaid, Omayma, and Ibraheem, A. and H. A, Osama, and K., Mohamed, and S. E. Z., Mamdouh,}, title = {Biochemical Study on Attenuating the Cardiotoxic Effect of Doxorubicin through Transforming into Mesoporous Nanoparticles Form}, journal = {Benha Veterinary Medical Journal}, volume = {35}, number = {1}, pages = {342-351}, year = {2018}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2018.39646}, abstract = {The aim ofthis study was transforming doxorubin by encapsulating it in mesoporous silica compared to free doxorubicin. In vivo study, free doxorubicin, mesoporous silica preparations were assessed by different measurements like particles size, zeta potential and TEM. Several parameters have been measured to assess the cardiotoxicity (Albumin, lactate dehydrogenase (LDH), Aspartate Aminotransferase (AST), Anticardiolipin IgG, Creatine kinase-MB (CK MB), C-reactive protein (CRP), Tumor necrosis factor (TNF α)).The experimental animals were divided into seven groups as follow: group (1) Control. (2) Group B:received doxorubicin. (3) Group C: mesoporousdoxorubicn. Rats injected withfree doxorubicin caused significantly elevated levels of all parameters measured compared to control group (albumin 4.435 ±0.085 vs 2.910 ±0.10, anticardiolipin 9.4 ± 0.256 vs 19.31 ±0.4391, AST 64.60 ± 1.79 vs 244.8 ± 9.86,  CK 14.04± 1.794 vs 73.25± 1.240, CRP 4.904±0.45 vs 23.96 ± 0.95, LDH 212±10.1 vs 754±16.4, TNF α 34.62±1.05 vs 126.6±6.25). In addition there was a significant variation of mesoporous silica results compared to free doxorubicin. Conclusion: There was a marked cardiotoxicity of doxorubicin could be decreased by transforming it into mesoporous form.}, keywords = {: cadiotoxicity,myocardial infarction,Doxorubicin,Mesoporous,Nanoparticles}, url = {https://bvmj.journals.ekb.eg/article_39646.html}, eprint = {https://bvmj.journals.ekb.eg/article_39646_f7ea5c49904909eb54a8a5bd87dc8d71.pdf} } @article { author = {Abd elmaksoud, H.A. and M. Abdelhamid, Omnia, and E.A. Ismaeel, Taheya and A. Elkharadly, Waheed.}, title = {The Biochemical Role of Pomegranate Peel Extract in Tissues Antioxidants of High Sucrose Stressed Rabbits}, journal = {Benha Veterinary Medical Journal}, volume = {35}, number = {1}, pages = {352-361}, year = {2018}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2018.39655}, abstract = {           Pomegranate (Pomg) fruit contains many phenolic compounds with high antioxidant activity, which may have useful health properties including flavonoids anthocyanins, flavonoids and hydrolysable tannins (punicalagin). The pomegranate peel includes tannins, gallagic acid ellagic acid and its esters of glucose and flavonoids. Oxidative stress is an imbalance between free radical production and the antioxidant defense system which could lead to lipid, protein and/or DNA alterations in this respect, the possibility exists that sucrose feeding facilitates oxidative damage. For the present study, the strong antioxidant activity of POMG on some antioxidant parameters in liver and heart of male rabbits exposed to high sucrose diet (HSD) induced oxidative stress, were investigated. Eighty (80) male rabbits at age of 4 weeks, weighting 500- 600 g. were used in this study. The rabbits were divided into four equal groups. 1) Control Normal group: kept on basal ration. 2) Stressed group (HSD): reared on the 25% sucrose concentration ration, daily for 4 months. 3) Pomegranate peel extract (PPE) group: reared on 25% pomegranate peel powder ration only, daily for 4 months. 4)Sucrose + PPE: kept on equal amounts of 25% sucrose and 25% PPE rations, daily for 4 months. Liver and heart samples were collected from all animal groups two times, after 2 and 4 months of experiment period. Levels of reduced glutathione (GSH) and activities of Catalase (CAT) and Glutathione-S-Transferase (GST) were determined in liver and heart tissues. Extent of oxidative stress was also assessed by liver and heart lipid peroxide L-Malondialdehyde (MDA) and Hydrogen peroxide H2O2. The obtained results revealed that, HSD supplementation exhibited a significant increase in: 1) Liver and heart MDA and H2O2 concentrations. PPE supplementation to stressed rabbits exhibited a significant decrease in the previously mentioned parameters. On contrast, HSD supplementation exhibited a significant decrease in liver and heart GSH, CAT and GST. Supplementation of PPE to stressed rabbits exhibited a significant increase in all mentioned parameters. From the obtained results, it could be concluded that, the potential of POMG as natural antioxidant act as a powerful agent against the harmful effects of sucrose induced oxidative stress.}, keywords = {pomegranate,sucrose%,Lipid peroxide,Oxidative Stress}, url = {https://bvmj.journals.ekb.eg/article_39655.html}, eprint = {https://bvmj.journals.ekb.eg/article_39655_1dd27901581eafba202b2139992acf14.pdf} } @article { author = {A.R. AbouZaid, Omayma and A Rashed, Laila and El-Sonbaty, S.M. and A. I., Aboel-Ftouh}, title = {Effect of Nanoselenium on Experimentally Induced Mammary Cancer in female Rats: Possible Role of TGF-β1}, journal = {Benha Veterinary Medical Journal}, volume = {35}, number = {1}, pages = {362-368}, year = {2018}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2018.39661}, abstract = {Nanoparticles combined with natural products could be an efficient tool in cell base cancer gene therapy. Guided treatments with nanoparticles and radiotherapy are a new approach in cancer therapy.   The current study evaluated the beneficial antitumor effects of SeNPs, γ-radiation together with selenium nanoparticles (SeNPs) against mammary gland cancer in female Rats}, keywords = {}, url = {https://bvmj.journals.ekb.eg/article_39661.html}, eprint = {https://bvmj.journals.ekb.eg/article_39661_2c7b00d0ebd7af2ac73e57efeea35237.pdf} } @article { author = {A.R. AbouZaid, Omayma and A Rashed, Laila and El-Sonbaty, S. M. and A. I, Aboel-Ftouh}, title = {Antitumor Effect of Mesenchymal Stem Cells from Murine in Breast Cancer in Female Rats}, journal = {Benha Veterinary Medical Journal}, volume = {35}, number = {1}, pages = {369-374}, year = {2018}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2018.39664}, abstract = {The aim of this study was to evaluate the effect of mesenchymal stem cells as a treatment against breast cancer via modulation TGF-β. Mesenchymal stem cells (MSCs) are recruited to the stroma of cancers. They interact with cancer cells to promote invasion and metastasis or to suppress tumor growth. The unique tumor-homing capacity of MSCs makes them a promising vehicle to deliver various anticancer agents.}, keywords = {}, url = {https://bvmj.journals.ekb.eg/article_39664.html}, eprint = {https://bvmj.journals.ekb.eg/article_39664_c7c4e0f4a38ee357076b343914d1accc.pdf} } @article { author = {Hussein, Samy A. and Azab, Mohamed E. and Abdelwahed, Sheryhan M.}, title = {Biochemical effect of curcumin on experimentally induced myocardial injury in rats}, journal = {Benha Veterinary Medical Journal}, volume = {35}, number = {1}, pages = {375-387}, year = {2018}, publisher = {Benha University; Faculty of Veterinary Medicine}, issn = {1110-6581}, eissn = {2974-4806}, doi = {10.21608/bvmj.2018.188897}, abstract = {Myocardial infarction (MI) is one of the main causes of death from cardiovascular disease. Curcumin was shown to exert potent antioxidant, anti-inflammatory, antiangiogenic, antimutagenic, anticoagulant and antidiabetic activities. This study was done to investigate the protective effects of curcumin in isoproterenol (ISO) induced myocardial injury in rats. Forty-eight rats were divided into 6 equal groups. Group I: (normal control) rats received no drugs. Group II :(curcumin) rats received curcumin (200 mg/kg b.wt/day) orally for 30 days. Group III: (acute myocardial infarction) rats injected ISO (20mg/kg b.wt /intraperitoneally) twice at the 29th and 30th days of experiment. Group IV: (chronic myocardial infarction) rats injected ISO (20mg/kg b.wt /intraperitoneally) twice at the 1st and 2nd days of experiment. Group V: (acute myocardial infarction + curcumin) rats received curcumin (200 mg/kg b.wt/day) for 30 days,  then ISO was injected twice, at an interval of 24 hours, on the 29th and 30th days .Group VI:  (chronic myocardial infarction + curcumin) rats injected ISO at the 1st and 2nd days of experiment and treated with curcumin for 30 days. Blood samples and heart tissue specimens were collected at the end of experimental period (30 days) for determination of serum Creatine kinase MB (CK-MB), Lactate dehydrogenase (LDH), cardiac Troponins (cTn) and Interleukin-1β (IL-1β). Moreover, heart tissues catalase (CAT), glutathione peroxidase (GPx), reduced glutathione (GSH) and L-Malondialdhyde (L-MDA) were also determined. The obtained results showed a significant increase in serum cardiac marker enzymes (CK-MB and LDH) activities, cTn and IL-1β concentrations in addition to L-MDA in heart tissues of ISO-induced myocardial injury in rats. However, GPx and CAT activities and GSH concentration in heart tissues of myocardial infarction induced in rats were markedly decreased. Curcumin treatment was able to protect rat myocardium against isoproternol induced myocardial ischemic damage and the protective effect was attributed to its antioxidant properties by inhibiting free radical generation.  }, keywords = {Isoproterenol,Curcumin,myocardial infarction,Oxidative Stress,cardiac marker enzymes}, url = {https://bvmj.journals.ekb.eg/article_188897.html}, eprint = {https://bvmj.journals.ekb.eg/article_188897_f20ab79b0e44f230a4e7b48b717e5707.pdf} }