ORIGINAL_ARTICLE
Assessment of pulmonary function test, acute phase proteins, cytokines and electrocardiographic changes in naturally occurring bovine respiratory disease of feedlot cattle calves
The aim of this study was to evaluate pulmonary function test, acute phase proteins, pro-inflammatory cytokines, electrolyte balance and electrocardiographic alterations in BRD affected calves compared to pen matched healthy control calves. A total of 30 calves (20 BRD affected calves and 10 apparently healthy calves) were used in this study. The BRD affected calves showed significant (P<0.05) reduction in blood pH, partial pressure of oxygen, oxygen saturation, calcium, phosphorus, magnesium, sodium and chloride, and significant (P<0.05) increase in partial pressure of carbon dioxide, total carbon dioxide and potassium. Acute phase proteins and Proinflammatory cytokines assessment revealed a significant (P<0.05) increase of serum amyloid A, haptoglobin, fibrinogen and interleukin-6 in diseased calves compared to healthy control calves. Electrocardiographic examination of BRD affected calves revealed a significant increase in heart rate with a significant (P<0.05) alteration in ECG wave trace parameters (P wave, QRS complex, T wave, PR interval, QT interval). In conclusion, measurement of pulmonary function test, APPs, cytokines and ECG could be used as valuable and early diagnostic tools for BRD diagnosis in feedlot cattle calves.
https://bvmj.journals.ekb.eg/article_103430_1bcec3c608a91bc4815f53deabd81252.pdf
2019-12-01
1
5
10.21608/bvmj.2019.16768.1089
BRD
pulmonary function
Acute phase proteins
Cytokines
eCG
mohammed
ramadan
dr.anwar72@gmail.com
1
department of animal medicine,faculty of veterinary medicine,benha university,Egypt
AUTHOR
Yassen
Abdel-Raoof
yassine.abdelraouf@fvtm.bu.edu.eg
2
department of animal medicine,faculty of veterinary medicine,benha university,Egypt
AUTHOR
mohamed
zeineldin
zeineldn@illinois.edu
3
department of animal medicine,faculty of veterinary medicine,benha university,Egypt
AUTHOR
Hossam Eldin
El Attar
hussam.elattar@fvtm.bu.edu.eg
4
department of animal medicine,faculty of veterinary medicine,benha university,Egypt
AUTHOR
abdelghany
abdelghany
abdelghany_hefnawy@yahoo.com
5
animal internal medicine department,faculty of veterinary medicine,menoufia university
AUTHOR
Mohamed
Ghanem
mohamed.ghanem@fvtm.bu.edu.eg
6
department of animal medicine,faculty of veterinary medicine,benha university,Egypt
LEAD_AUTHOR
ORIGINAL_ARTICLE
Effect of Thyme, Ginger and Boldenone as Growth Promoters on some biochemical blood parameters in white Male New-Zealand Rabbits
Objective: The present study was design to determine the biochemical changes effect of thyme, ginger and BOL as growth on male New-Zealand rabbits. Methods: Twenty four white New-Zealand male rabbits were selected at the age from 6-8 weeks old with an average body weight of 0.600 - 0.750 kg were used in the experimental investigation of this study. The rabbits were fed on a basal feed diet throughout the course of the experiment in the form of concentrated feed diet composed. All rabbits were randomly assigned into four equal groups (6 animals per each group). The biochemical analysis estimated total protein, lipid profile include: cholesterol and triglyceride, albumin, creatinine, blood serum aspartate aminotransferase (AST), alkaline phosphatase (ALP), oxidative stress marker such as glutathione (GSH) concentration, cytokine (IL-6), testosterone level and growth hormone were determine by using biolabo commercial kits with spectrophotometer, ELISA and radioimmunoassay machine at the biochemistry laboratory. Results: After one month treatments, no significance difference was found between the rabbits groups in initial body weight in the 1st administration compared 2nd administration. There was increasing of ALP and AST with ginger and increase of ALP with thyme even their positive effect on the other concerned parameters in our study. Ginger increase level of serum IL-6 levels and GSH concentrations, while thyme decrease its level in serum. Although Thyme supplementation group improved the growth performance and feed efficiency of rabbits in comparison to ginger supplementation group and control group.
https://bvmj.journals.ekb.eg/article_103431_e3a076ff47709449fcb8ea597ab2b106.pdf
2019-12-01
6
13
10.21608/bvmj.2020.18837.1127
growth promoters
thyme
Ginger
Boldenone
white Male New-Zealand Rabbits
Afaf
Desouky
desokyafaf@yahoo.com
1
Department of Biochemistry, Faculty of Veterinary Medicine, Benha University, Benha, Egypt
AUTHOR
Shereen
Abdelwahab
shereenabdelwahab@gmail.com
2
Department of Biochemistry, Faculty of Medicine, Benha University, Benha, Egypt
AUTHOR
Mohamed
Elshwaf
chemistsemsem@yahoo.com
3
Veterinarian
LEAD_AUTHOR
ORIGINAL_ARTICLE
Cytoprotective, anti-apoptotic and anti-inflammatory effects of lycopene against mercuric chloride -induced hepatorenal injury in rats: Involvement of TNF-α /NF-κβ/ and p53 signaling pathways
The purpose of this study was to evaluate the protective and anti-inflammatory effect of lycopene against (HgCl2) - induced hepato-renal injury and in rats. 30 rats were divided into 3 equal groups. (G1): rats administered distilled water. (G2): rats received 1/20th of LD50 of Hgcl2 orally (2 mg/kg b. w.t/day) over a period of 4 weeks. (G3): rats received (2 mg/kg b. w.t) Hgcl2+ lycopene at a dose of (20 mg/kg b. w.t/orally) for 4 weeks . Obtained results showed increase in serum ALT, AST and ALP activities, urea and creatinine concentrations and liver tissue MDA level in HgCl2 exposed rats. However, a significant decrease in liver tissue GSH concentration with down-regulation in hemoxygenase 1 (HO-1) gene expression and the anti-apoptotic protein Bcl-2 gene in kidney tissue were observed in (G2) Moreover, the qPCR results of kidney tissue revealed up-regulation of mRNA gene expressions levels of TNF-α, NF-kβ , Bax and p53 in (G2) when compared with control group. Administration of lycopene with HgCl2 (G2) caused improvement of all parameters towards its normal ranges. Various histopathological alterations were detected in kidneys and liver of rats treated with HgCl2. Interestingly, rats treated with lycopene +HgCl2 showed marked reduction in these pathological alterations in comparison to HgCl2 (G2). These results suggested that, the potential ameliorating role of lycopene as potent cytoprotective, anti-inflammatory and anti-apoptotic effects against HgCl2 induced hepato renal damage by inhibiting TNF-α mediated activation of the NF-κβ, Bax, and p53 signaling pathways and activation of (HO-1 and Bcl-2 )genes.
https://bvmj.journals.ekb.eg/article_103438_16060e7339fd8f4e52da05676a238264.pdf
2019-12-01
14
22
10.21608/bvmj.2020.23175.1159
Mercuric chloride
lycopene
apoptosis
Inflammatory mediators
histopathology
Mona
Abueldahab
m.dahab2050@hotmail.com
1
Veterinarian
LEAD_AUTHOR
Samy
Hussein
samyhussein@yahoo.com
2
Department of Biochemistry, Faculty of Veterinary Medicine, Benha University, Egypt
AUTHOR
Mohamed
Azab
mohamed.azab@fvtm.bu.edu.eg
3
Department of Physiology, Faculty of Veterinary Medicine, Benha University, Egypt
AUTHOR
Tahia
Ahmed
4
Department of Nutrition and Clinical Nutrition, Faculty of Veterinary Medicine, Benha University, Egypt
AUTHOR
Aziza
Amin
aziza.amin@fvtm.bu.edu.eg
5
Department of Pathology, Faculty of Veterinary Medicine, Benha University, Egypt
AUTHOR
ORIGINAL_ARTICLE
Isolation and genetic analysis of BoHV-1 gC gene isolated from animals suffering respiratory disease in Dakahlia, Egypt 2018
Objective of the present study was to isolate, identify and genetically characterize bovine herpes virus Type 1 (BoHV-1) from suspected cattle showing clinical symptoms of respiratory disease in Dakahlia, Egypt, September 2018.The virus from suspected nasal swabs and lung tissue samples was adapted onto specific pathogen free - embryonated chicken eggs (SPF - ECE).Pock lesions on the chorio-allantoic membrane (CAM) of SPF - ECE ranged from 1 to 2 mm in diameter and scattered all over the membrane that were more pronounced after the third passge. Sensitive PCR assay specific for BoHV-1 gC gene was also performed for molecular identification of the viral isolate and the gC PCR product was sequenced. Phylogenetic analysis of gC gene sequence identified and clustered our isolate with BoHV1.1. This highlights the isolation, rapid and sensitive detection of BoHV-1 virus from clinical cases and its subtyping by nucleotide sequencing and subsequent phylogenetic analysis which gives valuable information about the molecular epidemiology of BoHV-1 subtypes prevalent in the country.
https://bvmj.journals.ekb.eg/article_103422_ff67475cc2d31cdd8a36f7a6c47f8164.pdf
2019-12-01
23
27
10.21608/bvmj.2019.17295.1099
BoHV-1
ECE
PCR
Sequence
Phylogeny
Asmaa
Elashmawy
yara.remass@yahoo.com
1
virology department, animal health institute
LEAD_AUTHOR
Saad
Ali
ss_sharawi@yahoo.com
2
Department of virology , faculty of veterinary medicine, benha university
AUTHOR
ayman
El-Habbaa
aymanelhabbak@gmail.com
3
Department of Virology, Faculty of Veterinary Medicine, Benha University
AUTHOR
Iman
Bastawecy
iman_baz@yahoo.com
4
Virology Department, Animal Health Research Institute, Dokki, Giza, Egypt
AUTHOR
ORIGINAL_ARTICLE
Bacteriological and Molecular diagnosis of Most Common Bacteria Causing subclinical Mastitis in Cow
The aim of the study was using polymerase chain reaction (PCR) technique for detecting the most bacteria causing mastitis in cows as streptococci spp., Escherichia coli and Staphylococcus aureus by using Genus specific primers for each one. One hundred milk samples were collected from cases of subclinical bovine mastitis positive for California Mastitis Test (CMT). The samples were bacteriologically cultivated on to 5% sheep blood agar and also, on different types of media (MacConkey's agar plates, Edward's agar medium and mannitol salt agar) to isolate the major bacteria causing mastitis and identified biochemically. Finally, these bacteria were identified by using PCR technique with a specific primer for each genus. The PCR results revealed that E. coli 16/46 (34.7%), Staphylococcus aureus (S. aureus) 12/40 (30%) and streptococci spp. 11/39 (28.2%). Multiplex PCR for typing of streptococci spp. revealed that St. agalactiae 3/39 (7.69%) and St. dysgalactiae 8/39 (20.51%). In conclusion, in the present study, PCR analysis was used for diagnosis of major bacteria causing subclinical mastitis in cows by using specific primers for each one. The most prevalent bacteria causing subclinical mastitis were E. coli, S. aureus, S. dysgalactia and S. agalactia.
https://bvmj.journals.ekb.eg/article_103318_8f53705dc180d519b0c6dc3d21dead50.pdf
2019-12-01
28
32
10.21608/bvmj.2020.18716.1126
Bovine Mastitis
PCR
Staphylococcus aureus
streptococci spp
E. coli
Walaa
Saad
loloadam330@gmail.com
1
Central Diagnostic & Research Lab, Faculty of Veterinary Medicine, Kafrelsheikh University, Egypt
LEAD_AUTHOR
Ashraf
Abd El-Tawab
2
Department of Bacteriology, Immunology and Mycology, Faculty of Veterinary Medicine, Benha University
AUTHOR
Ashraf
Nabih
3
Animal Reproduction Research Institute (ARRI), Ministry of Agriculture, Giza, Egypt
AUTHOR
ORIGINAL_ARTICLE
Phenotypic and genotypic characterization of Campylobacter isolated from poultry
Campylobacteriosis incidence and proportion of Campylobacter strains resistant to antibiotic has been increasing worldwide in the last decades. Campylobacteriosis is considered as one of the major important zoonotic gastrointestinal diseases around the world. The aim of this study is isolation and biochemical characterization of Campylobacter from poultry. Campylobaceter isolation have been done on MCCDA media, motility have been detected by phase contrast microscope , morphology have been detected by Gram stain, and confirmed by MALDI-TOF MS and molecular confirmation by using cPCR by amplification of 16SrRNA gene. A total of 102 isolate of Campylobacter were isolated from (farm and small backyards located in different sources in El-Kalyobia , El-Monofia and El-sharkia Governorate) by conventional bacteriological methods from which 40 were C.jejuni and 62 were C.coli. All isolats have been confirmed as Campylobacter by MALDI-TOF MS and detection of 16SrRNA were detected by cPCR in all isolates. PCR is a useful molecular tool for identification and confirmation of Campylobacter, its Rapid, sensitive and specific than the culture methods the only disadvantage is that is expensive.
https://bvmj.journals.ekb.eg/article_103423_4da0bdf447acd47f7ac4bb243d124aed.pdf
2019-12-01
33
36
10.21608/bvmj.2020.22927.1156
Campylobaceter
Poultry
prevelance
wedad
Ahmed
drwedad_2009@yahoo.com
1
department of bacteriology, immunology and mycology,faculty of veterinary medicine ,Benha University
LEAD_AUTHOR
Ashraf
Abd El-Tawab
ashrafabdeltwab@yahoo.com
2
Bacteriology, Immunology, and Mycology, Faculty of Veterinary Medicine, Benha University, Benha, Egypt
AUTHOR
Fatma
Elhofy
fatmaelhofy@yahoo.com
3
Bacteriology, Immunology, and Mycology, Faculty of Veterinary Medicine, Benha University, Benha, Egypt
AUTHOR
Helmut
Hotzel
hotzel.helmut@fli.de
4
Friedrich loeffler institiute ,Jena ,Germany
AUTHOR
Mona
Sobhy
monagaber@yahoo.com
5
Department of Reproductive Diseases, ARRI, ARC, 12556 Giza, Egypt
AUTHOR
ORIGINAL_ARTICLE
Bacteriological evaluation of raw Catfish (Clarias gariepinus)
Fish is a worldwide distributed food commodity regarded an inexpensive source of protein especially in the developing countries like Egypt; it provides a good balance of protein, vitamins and minerals. In Egypt, catfish consumption is increased especially with its availability with reasonable price after expanding of its intensive farming; however, bacteria occur naturally on the skin, in the gut and in the slime of living fish without causing harm in healthy fish, unfortunately, it may migrate to its musculature associating with improper transportation, storage, and handling rendering it of potential hazards to consumers and wholesome of fish for consumption; therefore this study aimed to bacteriologically evaluatefifty random samples of catfish collected from retail and supermarkets in Benha city, Qalubiya governorate, Egypt. Results revealed that, the mean values of aerobic plate count (APC), total enterobacteriaceae, and total coliforms countswere 9.74×105, 3.25×103, and 2.35×103CFU\g, respectively; Escherichia coli (E. coli), Salmonellae, andYarseniaenterocoliticacould be detected in 18, 6, and 10% of examined samples, respectively, which went in further serological identification. The study indicated that the hygienic status of raw catfish is strongly related to source of collection, conditions of storage and handling; and recommended following proper hygienic properties of rearing, transportation, storage and handling.
https://bvmj.journals.ekb.eg/article_103428_981cc36b82ccc6a250c2ce167f557925.pdf
2019-12-01
37
41
10.21608/bvmj.2019.15669.1071
Bacteriological Evaluation
catfish
Benha City
Rabab
Rawash
rababomarsalah@gmail.com
1
Fac. Vet. Med., Benha Univ
LEAD_AUTHOR
Saad
Saad
saadms44@yahoo.com
2
Food control Dept., Faculty of Veterinary Medicine, Benha Univ., Egypt
AUTHOR
Fatin
Hassanin
fatinsaidhassanin@yahoo.com
3
Food control Dept., Faculty of Veterinary Medicine, Benha Univ., Egypt
AUTHOR
Mohamed
Hassan
mohamedhsn40@yahoo.com
4
Food Hygiene Department, Faculty of Veterinary Medicine, Banha university
AUTHOR
Ahmed
Maarouf
drahmed.maarouf@yahoo.com
5
Microbiology Dept., Animal Health Research Institute, Benha Branch
AUTHOR
ORIGINAL_ARTICLE
Assessment of Bacterial Evaluation of imported Frozen Meat.
One hundred random samples of American, Brazilian, Indian and Sudanese imported frozen meat 25 of each were collected from different shops and super markets at Cairo province to evaluate their bacteriological quality. The obtained results indicated that the mean values of APC of the examined imported frozen meat samples were 7.25x105, 1.53x106, 1.54x106, and 2.35x106 in American, Brazilian, Indian and Sudanese respectively while the mean count of psychotrophic of the examined samples were 1.02x106, 1.42x106 3.57x105 and 1.50x106 respectively , the mean values of staphylococci count in the same samples were 2.41x103, 1.50x104, 1.87x104, and 2.62x104 respectively. For the incidence of Aeromonas species in examined frozen meat , A .hydrophila were present in 20 %, 28 %,40% and 32% of American ,Brazilian, Indian and Sudanese meat respectively while A.Slmonicida were present in 21%,24%,20% and 20% in the same samples respectively on the other hand A. puctata were present in 8%,16%,8% and 12% of the examined samples respectively . A.sorbia also present in 8%,20%,4% and 8% of the same samples respectively finaly A. cavia were present in 8%,16%,4% and 4% of the examined samples respectively
https://bvmj.journals.ekb.eg/article_103317_8c9081bdb06f14c9b86ff1a8db22b9b2.pdf
2019-12-01
42
45
10.21608/bvmj.2019.18547.1123
Imported frozen meat
APC
psychotrophic
Staphylococci count
Aeromones
Mohamed
Hafez
dr.mohamedhafez80@gmail.com
1
Food Hygiene Dep., Fac. Vet. Med. Benha Univ
LEAD_AUTHOR
Hemmat
Ibrahim
hemmatibrahim@yahoo.com
2
Food Hygiene andControl Dept., Fac.Vet.Med.,Benha Univ
AUTHOR
Reham
Amin
rehamnour2007@yahoo.com
3
Food Hygiene andControl Dept., Fac.Vet.Med.,Benha Univ
AUTHOR
ORIGINAL_ARTICLE
Mycological assessment of quail meat in Menufia governorate ,Egypt .
This study was conducted to assess the mycological contamination of quail carcasses in Monofia governorate,. Total of 100 samples of fresh and frozen quail meat were taken from breast and thigh (50 of each) with skin and without skin (25 of each) from different retail shops for mycological examination and mycotoxin residues. The obtained results revealed that the highly contaminated samples in Mold and Yeast counts were in frozen quail meat of thigh with and without skin ones ( 1.21× 106± 4.59 × 105 , 1.77 ×106± 6.72× 105) &( 1.76× 106± 5.65× 105, 9.79× 105± 4.54× 105 ) (cfu/g), respectively. Furthermore, the highest incidence of mold serotypes was Aspergillus spp. Specially A. flavus in all samples . On the other hand , mycotoxins residue was higher in frozen samples than fresh ones. Breast without skin samples were the highest in mycotoxin residues than others (7.3±0.04, 3.8±0.04 ,1.9±0.02)(ug/l)for B1 , B2 and G1 in both fresh and frozen samples , respectively. The public health importance of these contamination and the suggestive hygienic measures to improve the quality and safety of quail meat were discussed.
https://bvmj.journals.ekb.eg/article_103429_392698c190dec9bbe35594ea0313119e.pdf
2019-12-01
46
49
10.21608/bvmj.2019.15790.1073
Quail meat
mold
Yeast and Mycotoxin
Rania
Ramadan
raniaramadan2020@yahoo.com
1
Fac. Vet, Med., Benha Univ.
LEAD_AUTHOR
Faten
Hasaneen
fatenhasaneen@yahoo.com
2
Food Hyg and Control ,Dept, Fac. Of Vet. Med.,Benha.Univ.
AUTHOR
Amani
Salem
amanisalem@yahoo.com
3
Food Hyg and Control ,Dept, Fac. Of Vet. Med.,Benha.Univ.
AUTHOR
Dina
El- Zahaby
dina@yahoo.com
4
Food Hyg and Control ,Dept, Fac. Of Vet. Med.,Benha.Univ.
AUTHOR
Rania
Ibrahim
rania@yahoo.com
5
Food Hyg and Control ,Dept, Fac. Of Vet. Med.,Benha.Univ.
AUTHOR
ORIGINAL_ARTICLE
Assessment of Histamine Residues in Smoked and Salted fish
Histamine is a member of a group of compounds known as biogenic amines; Biogenic amines are biologically active compounds normally produced by decarboxylation of free amino acids and are present in a variety of foods, eg fish, fish products, meat, cheese and fermented foods. The presence of biogenic amines in these foods is an indicator of food spoilage. Histamine content is an essential quality parameter in sea food quality. In the present study a total of 90 random samples of salted and smoked fish products represented by fesiekh, salted Sardine, and smoked herring (30 of each) were collected at various periods of times from different fish markets in Gharbia governorate, Egypt. The collected samples were labeled and preserved individually in an insulated ice box as well as transferred to the laboratory as quickly as possible. The weight of each sample was varied from 100-150 g and examined for the presence of histamine by ELISA. The results revealed that 46.7 %were acceptable and 53.3 % non-acceptable for fesiekh, 73.3% were acceptable while 36.7% non-acceptable for Salted Sardine and 70 % were acceptable and 30 % non-acceptable for Smoked herring.
https://bvmj.journals.ekb.eg/article_103439_b7689336d99287e60f6729e9da1a1060.pdf
2019-12-01
50
52
10.21608/bvmj.2020.19179.1128
histamine
ELISA
Salted and Smoked fish
Naglaa
Sakr
naglaa.sakr333@gmail.com
1
Veterinarian
LEAD_AUTHOR
Mohamed
Hassan
mohamedhsn40@yahoo.com
2
Food Hygiene Department, Faculty of Veterinary Medicine, Banha university
AUTHOR
Fahim
Shaltout
fahimshaltout@hotmail.com
3
Food Hygiene Department, Fac. Vet. Med., Benha University
AUTHOR
Nabila
Elsheikh
nabila_elsheikh@yahoo.com
4
Animal Health Institute, Tanta Branch
AUTHOR
ORIGINAL_ARTICLE
Antibacterial Effect of Zinc Oxide Nanoparticles in Fresh Meat
Practical application of ZnO-NPs (20nm) suspension with different concentrations (5mM, 8mM, and 10mM) were investigated to evaluate its antibacterial effect in fresh meat. A total number of 12 samples of fresh meat were collected from different abattoirs (150 gm. of each) in Gharbia Governorate, Egypt, under complete aseptic conditions and transferred without undue delay to the Lab to evaluate the efficacy of ZnO NPs as antibacterial agents in fresh meat. The obtained results indicated that ZnO-NPs had a significant inhibitory effect on the growth of APC and Staphylococcus aureus during 6 days of refrigerator storage at 4 0C. At zero day, the mean values of APC in the control group was 3.27x107±6.05x106cfu/g, while after treatment with ZnO-NPs 5mM, 8mM and 10mM the mean values were decreased to 1.67x107±9.11x106, 1.55x107±9.73x106, and 9.41x106±7.67x105cfu/g, respectively. By the 3rd day of refrigeration storage the control group showed complete spoilage. While the mean values of Staphylococcus aureus at zero day in control group was 3.09x107±2.33x107cfu/g, while after treatment with ZnO-NPs 5mM, 8mM and 10mM the mean values were highly decreased to 1.40x107±9.56x106, 1.79x106±4.70x105, and 8.05x105±9.51x105cfu/g, respectively. Accurately, ZnO-NPs with concentration 10mM showed the highest reduction percentage 99.5% and 99.85% to APC and Staphylococcal aureus, respectively, from 3.27x107±6.05x106to 1.64x105±2.47x104 and 3.09x107±2.33x107 to 4.61x104±4.44x104 respectively, compared to other concentrations (5mM and 8mM). Thus the best sensory quality was obtained by ZnO-NP 10mM indicating to the fact that the antibacterial activity of ZnO-NPs is concentration dependent.
https://bvmj.journals.ekb.eg/article_103427_2c8c18970f9429efda80a60456821a62.pdf
2019-12-01
53
56
10.21608/bvmj.2019.14596.1040
Nanotechnology
Zinc Oxide Nanoparticles
Antibacterial activity
aerobic plate count
Staphylococcus aureus
Elsaid
Saafan
elsaid.saafan@yahoo.com
1
Veterinarian
LEAD_AUTHOR
Reham
Amin
rehamnour2007@yahoo.com
2
Food Hygiene and Control Dept., Fac. Vet. Med., Benha Univ.
AUTHOR
Nesreen
Eleiwa
nesreenwow@gmail.com
3
Food Hygiene Dept., Animal Health Research Institute, Dokki, Giza, Egypt.
AUTHOR
Mohamed
El-Shater
soshater2020@yahoo.com
4
Food Hygiene Dept., Animal Health Research Institute &quot;Doki, Giza , Egypt
AUTHOR
ORIGINAL_ARTICLE
Comparative Analysis of Histamine Levels of Common Farm Fishes
Histamine fish poisoning (HFP) is a foodborne chemical intoxication caused by consuming a bacterially contaminated fish that are capable of producing histamine. Histamine may cause food intolerance and allergic reaction. The aim of this study is to investigate the occurrence and level of histamine in fish that are commonly consumed in Kafrelshiekh governorate and how to control it's level in those fishes. Accurately, 90 samples from three different species of fish represented by Mugil cephalus, Oreochromis niloticus and Clarias gariepinus (30 of each) were collected at different times from different farms in Kafrelshiekh governorate and examined for determination of their histamine levels "mg%" using Enzyme-Linked Immunosorbent Assay (ELISA). Histamine was detected in all fish samples with mean values as following 9.76 ± 0.12 in Mugil cephalus, 13.32 ± 0.17 in Oreochromis niloticus and 21.05 ± 0.26 in Clarias gariepinus with acceptability of 82.22 % according to Egyptian Organization for Standardization "EOS" (2005).
https://bvmj.journals.ekb.eg/article_103426_b6c84a8e4b8a272804a314a885df12b2.pdf
2019-12-01
57
58
10.21608/bvmj.2019.12533.1006
histamine
Mugil cephalus
Oreochromis niloticus and Clarias gariepinus
Mai
Elsherief
dr.mai.f.elsherief@gmail.com
1
Animal health research institute
LEAD_AUTHOR
Mohamed
Hassan
mohamedhsn40@yahoo.com
2
Food Hygiene Department, Faculty of Veterinary Medicine, Banha university
AUTHOR
Engy
Elbahy
engy.elbahy@yahoo.com
3
Food Hygiene Department, Animal Health Research Institute, Dokki, Giza
AUTHOR
ORIGINAL_ARTICLE
The Effects of Live Yeast (Saccharomyces cerevisiae) and Chitosan on Rumen Fermentation Function and Rumen Development in Male Balady Goats
The present study aimed at comparing the influence of dietary supplementation of live dried yeast Saccharomyces cerevisiae and chitosan on rumen fermentation and rumen development in male Balady goats. A total number of 12 castrated adult male Balady goats (bucks) aged one year old were allocated into three similar groups (4 males each). Concentrates were offered at rate of 3% of body weight. Experimental groups were control group fed on basal diet consists of concentrate mixture without any feed additives; S. cerevisiae group fed on basal diet with feed additive of S. cerevisiae at rate of 0.3% (3Kg/ ton concentrate); chitosan group fed on basal diet with feed additive of chitosan at rate of 0.2% (2kg/ ton concentrate). The obtained results showed a significant (p<0.05) increase in total volatile fatty acids concentrations (TVFA), total ruminal protozoal count (TPC), quantative morphmetrical analysis of ruminal papillae length, width and number of papillae / surface area (cm2) and significant (p<0.05) decrease ruminal ammonia nitrogen concentration in Saccharomyces group when compared with the control group. While, treated with S. cerevisiae or chitosan caused no significant variation (p>0.05)
https://bvmj.journals.ekb.eg/article_103432_b6b93b35b91694ac14115b427ab20dc1.pdf
2019-12-01
59
65
10.21608/bvmj.2020.20865.1141
Saccharomyces cerevisiae
Chitosan
rumen fermentation
Rumen papillae
Balady Goat
Abd-Elkader,
A. Y.
amgad.yousef@fvtm.bu.edu.eg
1
Faculty of Veterinary Medicine, Benha University.
LEAD_AUTHOR
M.
Azab
2
Department of Physiology, Faculty of Veterinary Medicine, Benha University.
AUTHOR
Randa
Esmail
3
Department of Physiology, Faculty of Veterinary Medicine, Benha University.
AUTHOR
Abeer
Nafeaa
abeer_nafeaa@yahoo.com
4
Department of Physiology, Faculty of Veterinary Medicine, Benha University.
AUTHOR
S.
Shousha
5
Department of Physiology, Faculty of Veterinary Medicine, Benha University.
AUTHOR
ORIGINAL_ARTICLE
The Effects of Live Yeast (Saccharomyces cerevisiae) and Chitosan on Digestion Coefficient and Molecular Biology of Main Cellulolytic Bacteria in Male Balady Goat
The present study throw lights on the comparing influence of dietary supplementation of live dried yeast Saccharomyces cerevisiae and chitozan on digestion coefficient and molecular biology of some cellulolytic bacteria in male Balady goats. A total number of 12 castrated adult male Balady goats aged one-year-old were allocated into three groups (4 males each). Concentrates were offered at rate of 3% of body weight. Experimental groups were control group fed on basal diet consists of concentrate mixture without any feed additives; Saccharomyces cerevisiae group fed on basal diet with feed additive of S. cerevisiae at rate of 0.3% (3 Kg/ ton concentrate); chitosan group fed on basal diet with feed additive of chitosan at rate of 0.2% (2 kg/ ton concentrate). The obtained results revealed significant increases (p< 0.05) in nutrient digestibility of dry matter (DM), crude protein (CP), ether extract (EE), crude fiber (CF), nitrogen free extract (NFE), and Ash and a significant (P≤0.05) up regulation of genes expression level in S. cerevisiae and chitosan groups when compared with the control except in EE showed non significant increase in chitosan group comparing with the control group. Thus, it is recommended to use S. cerevisiae to improve feed efficiency of diet, growth and multiplication of ruminal microbes.
https://bvmj.journals.ekb.eg/article_103433_8498abf579827f38715c867cdfeb4c9d.pdf
2019-12-01
66
71
10.21608/bvmj.2020.20869.1142
Saccharomyces cerevisiae
Digestion coefficient
Chitosan
cellulolytic bacteria
Balady Goat
Abd-Elkader,
A. Y.
amgad.yousef@fvtm.bu.edu.eg
1
Physiology Department, Faculty of Veterinary Medicine, Benha University.
LEAD_AUTHOR
M.
Azab
2
Physiology Department, Faculty of Veterinary Medicine, Benha University.
AUTHOR
Randa
Esmail,
3
Physiology Department, Faculty of Veterinary Medicine, Benha University.
AUTHOR
Abeer
Nafeaa
abeer_nafeaa@yahoo.com
4
Physiology Department, Faculty of Veterinary Medicine, Benha University.
AUTHOR
S.
Shousha
5
Physiology Department, Faculty of Veterinary Medicine, Benha University.
AUTHOR
ORIGINAL_ARTICLE
The effect of thyme (thymus vulgaris) extract on Escherichia coli in diarrheic calves with study of its immunological effect.
The current study was designed to examine the antibacterial effect of thyme (thymus vulgaris) on Escherichia coli in diarrheic calves and also the effect of the thyme as immunostimulant. Collection of 100 rectal swabs from 100 diarrheic calves was done for isolation and identification of E.coli and serotyping of the bacterial isolates followed by their antibiotic sensitivity testing and determination of MIC and MBC of thyme oil on them. Supplementation of thyme extract to friesian calves milk was done and examined its effect of on their immunity by antibodies titration of IgM, IgG and IgA in their serum. Results revealed that the incidence of E.coli was 58% and pathogenic E.coli was 46%.The most predominant serotype was O125. The highly resistance of the strains was against oxytetracycline (OT) and ampicillin (AMP). The thyme had antibacterial effect against E.coli strains by MIC ranged from 5 to 320 µg /ml but the MBC was negative for all strains and the thyme extract at 40 mg/kg body weight improved the immunity as it significantly (P≤0.05) increased IgM, IgG and IgA titre in calves serum.
https://bvmj.journals.ekb.eg/article_103421_3f90ee96024fbfd8caf21fbef909dafe.pdf
2019-12-01
72
76
10.21608/bvmj.2019.17212.1095
Keywords: Calves
thyme
E.coli
immunity
Fatma
Elhofy
fatmaelhofy@yahoo.com
1
Bacteriology, Immunology, and Mycology, Faculty of Veterinary Medicine, Benha University, Benha, Egypt
LEAD_AUTHOR
Ashraf
Abd El-Tawab
ashrafabdeltwab@yahoo.com
2
Bacteriology, Immunology, and Mycology, Faculty of Veterinary Medicine, Benha University, Benha, Egypt
AUTHOR
wael
wafa
drwailfatoh1973@hotmail.com
3
Department of Cattle Breeding, Animal Production Research Institute, El Gemisa station, Elgharbia, Egypt
AUTHOR
Yasmeen
Abd EL-Baset Bedawy
yassomamdouh2014@yahoo.com
4
bacteriology, immunology and mycolgy,faculty of veterinary medicine,benha university, benha,egypt, Department of Cattle Breeding, Animal Production Research Institute, El Gemisa station, Elgharbia, Egypt.
AUTHOR