ORIGINAL_ARTICLE
Synergistic antimicrobial activity of black pepper extract with some antibiotics combination on Escherichia coli isolated from chickens
Antimicrobial activity of methanol piper nigrum extract in combination with some antibiotics was investigated againstEscherichia coli isolated from chicken samples. The in-vitro antimicrobial sensitivity pattern of these isolates was doneand the intermediate isolates were checked for combination with methanol piper nigrum extract after detecting theirantimicrobial activities against these isolates by both disc diffusion test and MIC and the obtained data showed thatantimicrobial activities of amoxicillin, doxycycline, gentamicin, erythromycin and cefotaxime were enhanced incombination with methanol piper nigrum extract. The obtained data showed that enhancement by both disc diffusion testand MIC for methanol piper nigrum extract and each of the mentioned antibiotics in decimal assay of additivity ratio was(0.5 for amoxicillin, cefotaxime and 0.5 for extract and 0.4 for doxycycline, gentamicin, and erythromycin and 0.6 forextract) against E. coli isolates.
https://bvmj.journals.ekb.eg/article_31104_a7fdd34d721ef897ade3deaffd7d3d6c.pdf
2017-03-01
1
6
10.21608/bvmj.2017.31104
Antimicrobial combination
black pepper extract
Escherichia coli
antibiotics
Synergism
Ashraf
Abd EL-Tawab
1
Department of Bacteriology, Immunology and Mycology Fac. Vet. Med Benha University, Egypt
AUTHOR
Ahmed
Ammar
2
Department of Microbiology Fac. Vet. Med Zagazig University, Egypt
AUTHOR
Ahmed
Hamouda
3
Department of Microbiology Animal Health Research institute Egypt
AUTHOR
Fatma
El-Hofy
4
Department of Bacteriology, Immunology and Mycology Fac. Vet. Med Benha University, Egypt
AUTHOR
Abdelrahman
Elgamal
5
Department of Bacteriology, Immunology and Mycology Fac. Vet. Med Benha University, Egypt
AUTHOR
ORIGINAL_ARTICLE
Synergistic antimicrobial combination with cumin extract and some antibiotics on Staphylococcus aureus from chickens
In the present study, antimicrobial activity of methanol Cuminum cyminum extract (50mg/ml) in combination with someantibiotics were investigated against Staphylococcus aureus isolates. The antimicrobial sensitivity pattern of theseisolates in vitro was done and the intermediate isolates were checked for combination with methanol Cuminum cyminumextract after detecting their antimicrobial activities against these isolates by both disc diffusion test and MIC and theresults showed that the antimicrobial activities of rifampicin, enrofloxacin, doxycycline, gentamicin, and erythromycinwere enhanced in combination with methanol Cuminum cyminum extract the obtained data showed that enhancement byboth disc diffusion test and MIC for methanol Cuminum cyminum extract and each of mentioned antibiotics in decimalassay of additivity ratio (0.5 for rifampicin and erythromycin and 0.5 for extract ,0.7 for enrofloxacin, and 0.3 forextract,0.6 for doxycycline, gentamicin and 0.4 for extract) against S.aureus isolates
https://bvmj.journals.ekb.eg/article_31105_60ee9c367408cd556d42e0ad6b25294a.pdf
2017-03-01
7
12
10.21608/bvmj.2017.31105
Antimicrobial combination
Staphylococcus aureus
cumin extract
antibiotics
Synergism
Ashraf
Abd EL-Tawab
1
Department of Bacteriology, Immunology and Mycology Fac. Vet. Med Benha University, Egypt
AUTHOR
Ahmed
Ammar
2
Department of Microbiology Fac. Vet. Med Zagazig University, Egypt
AUTHOR
Ahmed
Hamouda
3
Department of Microbiology Animal Health Research institute Egypt
AUTHOR
Fatma
El-Hofy
4
Department of Bacteriology, Immunology and Mycology Fac. Vet. Med Benha University, Egypt
AUTHOR
Abdelrahman
Elgamal
5
Department of Bacteriology, Immunology and Mycology Fac. Vet. Med Benha University, Egypt
AUTHOR
ORIGINAL_ARTICLE
Incidence of Coliform and Staphylococcus aureus in ready to eat fast foods
A total of 100 random samples of ready- to – eat sandwiches of beef products of kofta , liver, shawerma, and sausageproducts (25 samples of each ) were collected from different fast food services in different districts at Kaliobia governorateto be examined bacteriologically for detection of Coliform and Staphylococcus aureus microorganisms. The obtainedresults in the present study indicated that the mean value of coliform counts (cfu/g) in the examined samples of ready- to- eat meat products were 2.5 x 103 ± 0.74×103 for beef kofta, 8.85x102 ±1.92x 102 for beef shawerma, 8 x103 ± 1.65 x 103for beef sausage, 9.0 x 104 ± 2.30 x 104 for beef liver, furthermore the coliform were detected in 60% of beef kofta, 40%of beef shawerma, 52% of beef sausage and 80% of beef liver. The obtained results in the present study indicated that thestaphylococcus aureus was detected in 32% of beef kofta, 44% of beef liver, 8% of beef shawerma and 16% of beafsausage .The obtained results in the present study indicated that the ready- to-eat liver sandwiches were morecontaminated with Staphylococcus aureus as compared with those of kofta, shawerma and sausage. The examinedsamples of ready-to-eat liver sandwiches showed high incidence of coliform than those obtained by kofta, sausage andshawerma.
https://bvmj.journals.ekb.eg/article_31106_78f2b5435cae236b717ff66fab61d98a.pdf
2017-03-01
13
17
10.21608/bvmj.2017.31106
ready- to – eat sandwiches
Coliform
Staphylococcus aureus
kofta
Liver
shawerma
sausage
Fahim
Shaltout
1
Food Control Department, Faculty of Veterinary Med., Benha University, Egypt
AUTHOR
Mohammed
Farouk
2
Food Hygiene Dept., Eldoki Lab., Animal Health Research Institute
AUTHOR
Hosam
Ibrahim
3
Food Hygiene Dept., Faculty of Veterinary Med., Alexandria University
AUTHOR
Mostafa
Afifi
4
Veterinary Hospital Faculty of Veterinary Med., Benha University
AUTHOR
ORIGINAL_ARTICLE
Incidence of E. coli and Salmonellae in ready to eat fast foods
A total of 100 random samples of ready- to – eat sandwiches of beef products represented by kofta, liver, shawerma andsausage products (25 samples of each) were collected from different fast food services in different districts at kaliobiaGovernorate to be examined bacteriologically for detection of Salmonellae and Escherichia coli. The perecentages ofSalmonellae in the examined samples of kofta, liver, shawerma and sausage products were 32%, 60%, 8% and 40%,respectively. The obtained results indicated that the incidence of E. coli was 20%, 8% and 32% and 40% in the examinedkofta, shawerma, sausage and liver samples, respectively
https://bvmj.journals.ekb.eg/article_31107_c9c949ba23f4d5458186e1d2a300c47c.pdf
2017-03-01
18
22
10.21608/bvmj.2017.31107
ready- to – eat sandwiches
E. coli
Salmonellae
kofta
Liver
shawerma
sausage
Fahim
Shaltout
1
Food Control Department, Faculty of Veterinary Med., Banha University, Egypt
AUTHOR
Mohammed
Farouk
2
Food Hygiene Dept., Eldoki Lab., Animal Health Research Institute
AUTHOR
Hosam
Ibrahim
3
Food Hygiene Dept., Faculty of Veterinary Med., Alexandria University
AUTHOR
Mostafa
Afifi
4
Veterinary Hospital Faculty of Veterinary Med., Banha University
AUTHOR
ORIGINAL_ARTICLE
Estimation of some biogenic amines on chicken meat products
Sixty random samples of raw thigh & breast, half cooked chicken shawerma & shiesh tawook, fully cooked chickenshawerma and fully cooked chicken shiesh tawook (10 of each) were collected from different markets in Gharbiagovernorates and analyzed for biogenic amines by HPLC. The obtained results indicated that the mean values ofhistamine, cadaverine and putrescine (mg/100g) were 10.88 ± 0.91, 12.65 ± 1.10 and 4.54 ± 0.32 for raw thigh, 4.54 ±0.32, 9.86 ± 0.87 and 9.86 ± 0.87 for raw breast 16.56 ± 1.24, 19.81 ± 1.52, and 11.41 ± 0.96 for half cooked shawerma,12.29 ± 0.97, 16.20 ± 1.08 and 9.98 ± 1.08 for half cooked shiesh tawook, 12.93 ± 1.03, 16.69 ± 1.33 and 8.01 ± 0.63 forfully cooked shawerma and 11.68 ± 0.97, 11.91 ± 0.97 and 7.47 ± 0.50 respectively for fully cooked shiesh tawook.According to the permissible limits recommended by EOS (2005) 10%, 30%, 20%, 20% and 20% of the examinedsamples of raw thigh, half cooked shawerma, half cooked shiesh tawook, fully cooked shawerma and fully cooked shieshtawook were unaccepted. All raw breast samples were accepted as a result of their histamine content. While, 20%, 40%,30%, 30% and 20% of the examined samples raw thigh, half cooked shawerma, half cooked shiesh tawook, fully cookedshawerma and fully cooked shiesh tawook were unaccepted. Moreover, raw breast samples were accepted as result oftheir cadaverine content respectively. Accurately 10% of half cooked shawerma were unaccepted while all examinedsamples were accepted as a result of putrescine content
https://bvmj.journals.ekb.eg/article_31108_5b634670d823bf60a9143bb1f68b3490.pdf
2017-03-01
23
28
10.21608/bvmj.2017.31108
biogenic amines
Chicken Meat Products
histamine
cadaverine
putrescine
Hemmat
Ibrahim
drhemmat01@yahoo.com
1
Food Control Dept., Faculty of Veterinary Medicine, Benha University
AUTHOR
Reham
Amin
rehamnour2007@yahoo.com
2
Food Control Dept., Faculty of Veterinary Medicine, Benha University
AUTHOR
Nesreen
Eleiwa
3
Food Hygiene Dept., Animal Health Research Institute, Tanta Branch
AUTHOR
Noha
Ahmed
4
Food Hygiene Dept., Animal Health Research Institute, Tanta Branch
AUTHOR
ORIGINAL_ARTICLE
Bacteriological and molecular studies of garlic effect on some virulence genes of Escherichia coli of chicken origin
Avian colibacillosis is the most significant infectious bacterial disease of poultry worldwide. Many antimicrobial-resistantEscherichia coli pathogens are increased due to drug overusing in veterinary medicine and animals feed. The objective ofthis study was to observe the in vitro inhibitory effect of garlic (Allium sativum) as alternative natural agent againstEscherichia coli isolates and their virulence genes expression. The antimicrobial effects of aqueous and ethanolic garlicextracts against multidrug-resistant (MDR) E. coli isolates were studied using agar well-diffusion method as well as theminimum inhibitory concentration (MIC) of garlic and consequently their subinhibitory concentration (SIC) (theconcentration of garlic that did not inhibit E. coli growth after 24 hr. incubation at 37ºC) were calculated by a modificationof broth macrodilution method. The E. coli virulence was assessed via mRNA expression of their genes such as increasedserum survival (iss), verotoxine (vt2e) and intimin (eaeA) before and after garlic treatment using reverse transcriptase realtime PCR. One hundred and thirteen (46.12%) of 245 isolates were identified as E. coli that were highly recorded inintestinal samples as 64.60% followed by liver 24.77% then heart 10.6%. The most chemotherapeutic resistant 14 E. coliisolates showed high garlic susceptibility rates mainly for aqueous extract and had nine different serotypes, the mostpredominant one was O146 of 21.42%. The complete inhibition of and downregulating of eaeA and vt2e genes expressionwere proved respectively in the E. coli treated isolates with garlic SIC (1%). In conclusion, the garlic supplementationthrough feed can reduce infection by E. coli via decrescent their toxin production and may be the adhesion ability to theintestinal mucosa of the host either animal or human
https://bvmj.journals.ekb.eg/article_31109_c21aba465769d09e885206c9bd4f4f97.pdf
2017-03-01
29
40
10.21608/bvmj.2017.31109
Garlic (Allium sativum)-MIC– E. coli- virulence gene
Ahlam
Gharib
1
Microbiology Department, Fac. of Vet. Med., Zagazig Univ.
AUTHOR
Mokhtar
Sleim
2
Animal Health Research Institute (Zagazig branch)
AUTHOR
Mohamed
Hegazy
3
Agricultural Microbiology Department, Fac. of Agr., Zagazig Univ.
AUTHOR
Abdel-wahab
M.
4
Microbiology Department, Fac. of Vet. Med., Zagazig Univ.
AUTHOR
Sara
Adly
5
Animal Health Research Institute (Zagazig branch)
AUTHOR
ORIGINAL_ARTICLE
Protective impacts of Cupressus sempervirens leaves extracts against paracetamol hepatotoxicity
The main objective of this study is to evaluate the antioxidant activity of Cupressus sempervirens leaves extract invitroand rat model in which paracetamol was used to induce hepatic damage. The antioxidant and anti-quorum sensingactivities of eight extracts were studied. Total phenolic compounds and scavenging radical effect on 2, 2-diphenylpicrylhydrazyl (DPPH) were investigated, (the specific phenolic and flavonoid composition quantification forhydrolyzed ethyl acetate fraction pH 4 were performed by HPLC E-vanilic, Alpha-coumaric, Salicylic and Ferulic werefound as the major phenols. Narengin, Hisperidin, Quercetrin and Hispertin were found as the major flavonoids.Biological experiment was carried out for thirty days. Results revealed that a significant increase in serum ALT, AST,ALP, Total bilirubin, TG, Total cholesterol, (LDLC) and nitric oxide in rats treated with paracetamol. Howeveradministration of Cupressus sempervirens in paracetamol induced liver toxicity in rats exhibited a significant decreasein all mentioned parameters on the other hand a significant decrease in serum total protein albumin concentration,(HDLC), Catalase, glutathione peroxidase (GPX), Glutathione –S-transferase (GST) and Glutathione reduced wereobserved in paracetamol induced hepatic toxicity in rats when compared with control normal group. MeanwhileCupressus sempervirens leaves resulted in significant in all mentioned parameter and when enhanced the activity ofantioxidant enzymes in liver tissue. It could be concluded that, inhibition of peroxidation, inflammation and oxidativestress and enhanced antioxidant status in rat liver tissue by Cupressus sempervirens suggest the potential efficacy ofCupressus sempervirens as an addition Hepatoprotective, anti-inflammatory and anti-hepatotoxic agent in treatment ofliver toxicity
https://bvmj.journals.ekb.eg/article_31110_339bb4dd477aa06b482092eee4d4a882.pdf
2017-03-01
41
49
10.21608/bvmj.2017.31110
Cupressus sempervirens leaves
antioxidant
Hepatoprotective
Paracetamol
Ahmed
Salman
1
Biochemistry Dept. Fac. Agric., Benha University, Egypt
AUTHOR
Ibrahim
Abd El-Aleem
2
Biochemistry Dept. Fac. Agric., Benha University, Egypt
AUTHOR
Ahmed
Abd-El Rahman
3
Biochemistry Dept. Fac. Agric., Benha University, Egypt
AUTHOR
Tarek
Elhusseini
4
General surgery and liver transplantation Dept. Fac. Of Medicine. Mansoura University, Egypt
AUTHOR
Abd Allah
El-Hadary
5
Biochemistry Dept. Fac. Agric., Benha University, Egypt
AUTHOR
ORIGINAL_ARTICLE
Bacterial profile of bovine carcasses at abattoir
A grand total of 180 swabs representing 60 bovine carcasses were collected under aseptic conditions from different parts(hind quarter, abdomen and fore quarter) of the carcass surface at 2 Basatin abattoir halls in summer. Collected sampleswere subjected to bacteriological examination and the results of APC in the following region A1, A2, A3, B1, B2, B3 inhall 1 were 3.8 x104 ± 3.4x 10, 3.5 x104 ± 2.1 x103, 4.7 x104 ± 3.1 x103, 3.3 x104 ± 1.1 x103, 3.3 x104 ± 4.4 x103, 4.4 x104± 4.3 x103. Concerning hall 2, such results were 4.6 x104 ± 9.9 x103, 5.7 x104 ± 9.2 x103, 4 x104 ± 2.7 x103, 6.4 x104 ±3.9 x103, 3.3 x104 ± 4.6 x103 and 6 x104 ± 4.8 x103 respectively. On the other hand, Salmonella Typhimurium wasisolated from the carcass swabs at a percentage 2.75% from A1, A2, B2, B3 parts. The analysis was carried out and therecommendations showed that the Salmonella Typhimurium antigenic structure acc. To serological identification wassomatic (O) antigen 1, 4, (5), 12 and flagellar (H) antigen phase 1:1, phase 2:2.
https://bvmj.journals.ekb.eg/article_31111_5d75686fb3d3536d0a00bc3a9e22d3d7.pdf
2017-03-01
50
53
10.21608/bvmj.2017.31111
Bovine carcass
Salmonella
abattoir
bacterial count
meat contamination
Hassanein
S.
1
Faculty of Vet. Medicine Benha University
AUTHOR
Shaltout
A.
2
Faculty of Vet. Medicine Benha University
AUTHOR
El Shater
A.
3
Animal health research Institute, Doki, Giza
AUTHOR
ElKewaiey
A.
4
Animal health research Institute (Damanhor lab.)
AUTHOR
El-Minisy
M.
5
Animal health research Institute, Doki, Giza
AUTHOR
ORIGINAL_ARTICLE
Demonstration of Lead and Cadmium residues in Street vended foods
One hundred and thirty-five random samples of street vended foods represented by kofta, hawawshi and liver (45 of each)were collected from Benha (15 of each product), Tukh (15 of each product) and Moshtohor (15 of each product) inKalyobia governorate for determination of their content of lead and cadmium. The obtained results recorded that theoccurrence of cadmium in Benha city were 26.67, 40.00 and 46.67% in the kofta, hawawshi and liver samples,respectively. In Tukh center were 26.67, 46.67 and 60.00% in the kofta, hawawshi and liver samples, respectively.Whereas in Moshtohor village were 40.00, 53.33 and 73.33% in the kofta, hawawshi and liver samples, respectively. Inaddition, our results showed that the percentage of occurrence of lead in Benha city were 20.00, 60.00 and 66.67 % in thekofta, hawawshi and liver samples, respectively. In Tukh center were 33.33, 40.00 and 53.33 % in the kofta, hawawshiand liver samples, respectively. Whereas in Moshtohor village were 20.00, 33.33 and 40.00 % in the kofta, hawawshi andliver samples, respectively. It could be inferred that regarding the products contamination, the highest cadmiumcontamination was in liver followed by hawawshi then kofta. Whereas kofta showed the highest lead contaminationfollowed by hawawishi then liver. Regarding the locality, Moshtohor represented the highest contamination of bothcadmium and lead followed by Tukh then Benha city
https://bvmj.journals.ekb.eg/article_31112_6b778af3cb6512097bf4154194507931.pdf
2017-03-01
54
58
10.21608/bvmj.2017.31112
Lead
cadmium
Liver
street vendors
meat products
Suzan
Elsisy
1
Animal Health Research Institute, Benha Branch
AUTHOR
Ali
Ali
2
Animal Health Research Institute, Benha Branch
AUTHOR
Hemmat
Ibrahim
drhemmat01@yahoo.com
3
Food Hygiene Control Department, Faculty of Veterinary Medicine, Benha University
AUTHOR
Mohamed
Hassan
mohamed.hassan@fvtm.bu.edu.eg
4
Food Hygiene Control Department, Faculty of Veterinary Medicine, Benha University
AUTHOR
ORIGINAL_ARTICLE
Biochemical effect of cranberry extract on experimentally induced myocardial infarction
Myocardial infarction (MI) is characterized by an inequity of coronary blood supply and demand, which results inmyocardial ischemic injury and damages the cardiomyocytes. Isoproterenol (ISO) is a synthetic catecholamine causetoxicity leading to severe stress in the myocardium of experimental animals. The aim of the present article is to investigatecardioprotective activity of cranberry extract against ISO-induced cardiotoxicity in adult rats. Oral administration ofcranberry extract at a concentration of 75 and 150 mg/kg b.wt. daily for 28 days showed a significant protection againstinducedalteration in plasma total cholesterol (TC), triacyclglycerols (TG), high density lipoprotein (HDL-C), low densitylipoprotein (LDL-C), tumor necrosis factor-alpha (TNF-α) and nitric oxide (NO) as well as cardiac superoxide dismutase(SOD), glutathione peroxidase (GPx), reduced glutathione (GSH), catalase (CAT) levels. In addition, cranberry extractreduced plasma Creatine kinase-MB (CK-MB), lactate dehydrogenase (LDH) and aspartate aminotransferase (AST) aswell as cardiac thiobarbituric acid reactive substances (TBARS) levels (MDA) as compared to control group. Inconclusion, cranberry extract renders resiliency against isoproterenol cardiotoxicity due to its antioxidant and free radicalscavenging activity
https://bvmj.journals.ekb.eg/article_31113_9407ead6299a6b0ee2c87337b83d46fc.pdf
2017-03-01
59
66
10.21608/bvmj.2017.31113
Cranberry extract
Isoproterenol
myocardial infarction
antioxidant
Hussien
Ali
1
Department of Biochemistry, Faculty of Veterinary Medicine, Benha University
AUTHOR
Omnia
Mahmoud
2
Department of Biochemistry, Faculty of Veterinary Medicine, Benha University
AUTHOR
Mohammed
Hussein
3
Department of Biochemistry, Faculty of Pharmacy, October 6 University
AUTHOR
Mariam
Mohammed
4
Department of Biochemistry, Faculty of Pharmacy, October 6 University
AUTHOR
ORIGINAL_ARTICLE
Biochemical effect of resveratrol on Lipids profile and hepatic oxidative stress in experimentally induced obesity in female rats
This study was designed to evaluate the effect of prolonged intake of resveratrol on lipid metabolism, liver enzymes andL-malondialdehyde(L-MDA) in obesity -induced in female rats by feeding high fat diet. Ninety female albino rats weredivided into six equal groups of 15 rats each. Group Ι: rats fed normal control diet all over the periods of experiment (for12 weeks). Group II: rats fed the control normal diet and administered resveratrol orally (0.1 mg/kg b.wt., orally) for 6weeks. Group III: rats received the control normal diet and received resveratrol once daily (0.5 mg/kg b.wt., orally) for 6weeks. Group IV: rats fed high fat diet (HFD) and received no drugs all over the periods of experiment (for 12 weeks).Group V: rats received HFD and administered resveratrol (0.1 mg/kg b.wt./day, orally) for 6 weeks. Group VI: rats fedHFD and administered resveratrol (0.5 mg/kg b.wt./day, orally). Blood samples were collected from all rats at 2, 4 and 6weeks from the onset of resveratrol administration for determination of serum total cholesterol(TC), Triacylglycerols(TAG), high density lipoprotein cholesterol (HDL-c), low density lipoprotein cholesterol (LDL-c), very low densitylipoprotein cholesterol (VLDL-c), alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkalinephosphatase (ALP), and erythrocyte L-malondialdehyde (L-MDA).The obtained results showed, significant elevation ofserum TC, TAG, LDL-c, VLDL-c, ALP,AST, ALT, L-MDA in obese rats as compared to rats fed normal control diet.While administration of resveratrol to HFD-fed rats tended to improve hepatic oxidative stress and dyslipidemia throughimproving total cholesterol and triacylglycerols and liver markers enzyme activities. These results suggest that, resveratrolis effective in improving the obesity and its associated many important complications such as diabetes mellitus andcoronary heart disease and liver disease
https://bvmj.journals.ekb.eg/article_31114_1a0cc4bb23c9aab29b10cf3dfed4e71c.pdf
2017-03-01
67
74
10.21608/bvmj.2017.31114
Resveratrol
Obesity
Lipids Profile
liver enzymes
L-malondialdehyde
Samy
Hussein
samyaziza@yahoo.com
1
Biochemistry Department, Faculty of Vet. Med., Benha University, Egypt
AUTHOR
Afaf
Abdel-Magid
2
Biochemistry Department, Faculty of Vet. Med., Benha University, Egypt
AUTHOR
Fareed
Fareed
3
Biochemistry Department, Faculty of Vet. Med., Benha University, Egypt
AUTHOR
ORIGINAL_ARTICLE
Histamine as biogenic amine residue in imported frozen fish
A total of ninety random samples of imported frozen fish represented by Mackerel, Herring and Saurus (30 of each) fromtwo different countries namely A and B (15 samples of each) were collected from different fish markets in Menoufiyagovernorate. Each sample was weighed about 150 g and stored at -18oC. The collected samples were packed separatelyin sterile polyethylene bag and transferred directly to the laboratory with minimum delay for determination of their toxicresidues. The obtained results recorded that the occurrence of histamine in both country (A) and in country (B) were100% in the Mackerel, Herring and Saurus samples. The mean value of histamine in the country (A) samples were 38.14± 2.93, 31.67 ± 2.15 and 19.05 ± 1.68 mg/kg in the Mackerel, Herring and Saurus, respectively. The average concentrationof histamine in country (B) samples were 33.96 ± 2.45, 24.51 ± 1.72 and 12.83 ± 1.19 mg/kg in the Mackerel, Herringand Saurus, respectively. It could be inferred that regarding the products contamination, the highest histaminecontamination was in mackerel followed by herring then saurus. Regarding the locality, country (A) represented thehighest contamination of histamine followed by country (B)
https://bvmj.journals.ekb.eg/article_31115_408167c45213878cd874ef538bf777f9.pdf
2017-03-01
75
78
10.21608/bvmj.2017.31115
Mackerel
Herring
Saurus
histamine
Hassan
A.
1
Food Hygiene Control Department, Faculty of Veterinary Medicine, Benha University
AUTHOR
El- Shater
A.
2
Animal Health Research Institute, El-Doki
AUTHOR
Waly
A.
3
General Authority for Veterinary Services, Menoufiya, Berket El-Sabaa
AUTHOR
ORIGINAL_ARTICLE
Demonstration of Cadmium and Lead residues in Imported Frozen Fish
A total of ninty random samples of imported frozen fish represented by Mackerel, Herring and Saurus (30 of each)imported from two different countries namely A and B (15 samples of each fish species for each country) were collectedfrom different fish markets in Menoufiya governorate. The obtained results recorded that the occurrence of cadmium incountry (A) were53.33, 40.00 and 33.33% in the Mackerel, Herring and Saurus samples, respectively. In country (B) thecadmium occurrence was 40, 26.67 and 20.00% in the Mackerel, Herring and Saurus samples, respectively. In addition,our results recorded that the percentage of occurrence of lead in country (A) were 66.67, 66.67and 53.33% in theMackerel, Herring and Saurus samples, respectively; while in country (B) were 60, 46.67 and 40 % in the Mackerel,Herring and Saurus samples, respectively. It could be inferred that regarding the products contamination, the highestcadmium and lead contamination was in mackerel followed by herring then saurus. Regarding the locality, country (A)represented the highest contamination of both cadmium and lead followed by country (B).
https://bvmj.journals.ekb.eg/article_31116_988136b55bf7ec9b96cf8b1988090d43.pdf
2017-03-01
79
82
10.21608/bvmj.2017.31116
Mackerel
Herring
Saurus
cadmium and lead
Hassan
A.
1
Food Hygiene Control Department, Faculty of Veterinary Medicine, Benha University
AUTHOR
El- Shater
A.
2
Animal Health Research Institute, El-Doki
AUTHOR
Waly
A.
3
General Authority for Veterinary Services, Menoufiya, Berket El-Sabaa
AUTHOR
ORIGINAL_ARTICLE
Survival of Escherichia coli O157:H7 during processing and ripening period of Keş Cheese
In this study the survival of Escherichia coli O157:H7 during the production process and ripening period of Keş cheesewas investigated. Three vats containing equal amounts of raw milk were inoculated with 102, 104, 106 cfu mL-1 E. coliO157:H7, respectively andone vat remained uninoculated. Keş cheese was produced from both inoculated anduninoculated milks with the traditional method of the region. Cheese samples ripened at 6°C for 90 days and E. coliO157:H7 enumarations were done from uninoculated milk, inoculated milks, curds and different stages of ripening period.Most Probable Number technique was used for the analysis of E. coli O157:H7. Classical culture methods were used forthe detection of aerobic mesophilic bacteria, lactococci and coliform bacteria. Also, control cheese was analysed for dry matter, salt content in dry matter and pH value. Elimination times were 45 days for 102 cfu mL-1, 75 days for 104 cfu mL-1, and 90 days for 106 cfu mL-1 of E. coli O157:H7. The final dry matter, salt content in dry matter and pH value were 82.52 %, 3.16 % and 4.58, respectively. Consequently, Keş cheese should be ripened at least 90 days in order to beconsidered as safe for public health.
https://bvmj.journals.ekb.eg/article_31117_194774fa2ef921930908fa0ece9697e9.pdf
2017-03-01
83
88
10.21608/bvmj.2017.31117
Elimination times
Escherichia coli O157:H7
Keş cheese
traditional cheese
dairy producs
Özen
Yurdakul
1
Department of Food Hygiene and Technology, Faculty of Veterinary Medicine, Mehmet Akif Ersoy University, Istiklal Campus, 15030 Burdur, Turkey
AUTHOR
Seval
Kırdar
2
Department of Food Processing, Milk and Dairy Products Technology Programme, Mehmet Akif Ersoy University, Istiklal Campus, 15030 Burdur, Turkey
AUTHOR
Aylin
Doğru
3
Department of Food Hygiene and Technology, Faculty of Veterinary Medicine, Kırıkkale University, 71450 Kırıkkale, Turkey
AUTHOR
Erhan
Keyvan
4
Department of Food Hygiene and Technology, Faculty of Veterinary Medicine, Mehmet Akif Ersoy University, Istiklal Campus, 15030 Burdur, Turkey
AUTHOR
ORIGINAL_ARTICLE
E. coli strains producing Shiga toxin in cattle carcasses at abattoir level
A total of one hundred random swab samples were collected from the cattle carcasses slaughtered at four differentabattoirs located in Menufia governorate namely A, B, C and D (25 of each). The sampling site was randomly taken fromeach carcass inside the abattoir. The collected samples were examined for presence of E. coli and their virulence genesusing PCR technique. Incidence of E. coli isolated from the examined cattle swabs samples taken from abattoir A was E.coli (O55: H7, O111: H2, O126 and O114: H21) 4 % for each, E. coli (O111: H2) was 4 % and E. coli (O126) 4%. Whilein case of abattoir B E. coli (O86, O124 and O126) 4 % for all and E. coli (O119: H4 and O26: H11) 8% for both. On theother hand, results of abattoir C was E. coli (O111: H2 8% and O26: H11 12%), E. coli (O86, O114: H21, O124 andO142) 4% of each. But in case of abattoir D was E. coli O26: H11 16%, E. coli (O111: H2 O55: H7 and O126) 8% foreach, E. coli (O44: H18 and O125: H21) 4% for both. We found also that Shiga- toxin 1 gene (stx1) virulence gene waspresent in O26: H11, O44: H18, O55: H7, O111: H2, O119: H4, O126 and O128: H2. On the other hand, Shiga- toxin 2gene (stx2) virulence gene was present in O26: H11, O44: H18, O86, O111: H2, O114: H21, O119: H4, O125: H21 andO142. While intimin gene (eae) virulence gene was present in O26: H11 and O111: H2. Also haemolysin gene (hylA)gene was present in O26: H11, O55: H7 and O111: H2.
https://bvmj.journals.ekb.eg/article_31120_9b0608cf401cb0deb4ae181912e3e279.pdf
2017-03-01
89
93
10.21608/bvmj.2017.31120
Cattle carcasses
E. coli
Virulence genes
Shiga- toxin
Saad
S.
1
Department of food Hygiene, Fac. Vet. Med, Benha University
AUTHOR
Hassan
A.
2
Department of food Hygiene, Fac. Vet. Med, Benha University
AUTHOR
Nahla
Abou El-Roos
3
Food Hygiene Dept., Animal Health Research Institute Shebin El- Kom branch
AUTHOR
Gaafar
H.
4
Food Hygiene Dept., Animal Health Research Institute Shebin El- Kom branch
AUTHOR
ORIGINAL_ARTICLE
Staphylococcal contamination of cattle carcasses with particular reference to Staph. aureus enterotoxins
A total of one hundred random swab samples were collected from the cattle carcasses slaughtered at four differentabattoirs located in Menufia governorate namely A, B, C and D (25 of each). The sampling site was randomly taken fromeach carcass inside the abattoir. Mean values of total Staphylococci count (cfu/cm2) of the tested cattle carcass swabsamples obtained from abattoir A, B, C and D were 5.79×102 ± 1.14×102, 1.01×103 ± 0.37×103, 4.58×103 ± 0.92×103 and6.94×103 ± 1.21×103, respectively. Isolates serotypes were Staph. Aureus (12%), Staph. Epidermidis (4%), Staph.Intermedius (0%), Staph. Saprophyticus (0%), Staph. Capitis (0%) and Micrococcus spps (4%) in abattoir A. While thefore mentioned serotypes were recorded in abattoir B in percentage of (20%), (8%), (0%), (4%), (0%), (0%), respectively.On the other hand, the mentioned serotypes were recorded in abattoir C in percentage of (24%), (0%), (0%), (4%), (0%),(8%). Finally, in case of abattoir D the serotypes mentioned above found in percentage of (40%), (8%), (4%), (0%), (4%),(0%), respectively. Enterotoxin type A was produced by 2 strains of Staph. aureus at age of 13.33%. While enterotoxintype B, D, A+C and A+D were produced by single strain of Staph. aureus for each at age of 6.67%. On the other hand,enterotoxin type C is produced by 3 strains of Staph. aureus at age of 20%. Finally, there were 8 strains of Staph. aureuswas not capable of producing enterotoxin at age of 53.33%.
https://bvmj.journals.ekb.eg/article_31121_1c5a6608bd85d393defa1c21e0adbbe4.pdf
2017-03-01
94
98
10.21608/bvmj.2017.31121
Cattle carcasses
Abattoirs
Staphylococci count
Staph. aureus virulence genes
enterotoxin
Saad
S.
1
Department of food Hygiene, Fac. Vet. Med, Benha University
AUTHOR
Hassan
A.
2
Department of food Hygiene, Fac. Vet. Med, Benha University
AUTHOR
Nahla
Abou El-Roos
3
Food Hygiene Dept., Animal Health Research Institute Shebin El- Kom branch
AUTHOR
Gaafar
H.
4
Food Hygiene Dept., Animal Health Research Institute Shebin El- Kom branch
AUTHOR
ORIGINAL_ARTICLE
1Biochemistry Department, Faculty of Vet. Med. Benha Univ., Egypt.
The present study was to determine the effect of aged garlic extract (AGE) on alleviating diabetic cardiomyopathy (DCM)in rats. An experimental diabetic rat model was induced by a single dose (60mg/kg body weight) of I.P injection ofstreptozoticin (STZ). AGE was orally administrated at a dose of 500 mg/kg body weight. Metabolic profiles include serumglucose, insulin, total cholesterol and triacylglycerols and myocardial enzymes activities as creatine kinase-isoenzyme(CK-MB), lactate dehydrogenase (LDH) and aspartate aminotransferase (AST) were determined. The obtained resultsindicated that administration of AGE increased the body weight and insulin concentration, meanwhile, significantdecrease in serum glucose concentration, CK-MB and LDH activities when compared with untreated induced diabeticgroup. Total cholesterol, triacylglycerols and AST showed non-significant changes in serum values in AGE treateddiabetic rats in comparing with STZ-induced diabetic group. From the obtained results it could be concluded that, AGEhas great potential therapeutic effect in the diabetic cardiomyopathy and other complications of coronary heart disease
https://bvmj.journals.ekb.eg/article_31160_130d184143cc081463cd7d7d2c87176c.pdf
2017-03-01
99
103
10.21608/bvmj.2017.31160
Diabetic cardiovascular diseases
aged garlic extract
myocardial enzymes
Afaf
Abdel Mageid
1
Biochemistry Department, Faculty of Vet. Med. Benha Univ., Egypt
AUTHOR
Mohamed
Abou Salem
2
Biochemistry Department, Faculty of Vet. Med. Benha Univ., Egypt
AUTHOR
Nancy
Salaam
3
Department of Forensic medicine and toxicology, Faculty of Vet. Med. Benha Univ., Egypt
AUTHOR
ORIGINAL_ARTICLE
Pathological studies on effects of ivermectin on male and female rabbits
Ivermectin considered natural fermentation product derived from soil bacterium streptomyces avermitis and itis abroad spectrum insecticide, acaricide and anthelimintic. This studies carried out in order to investigate thehistopathological effects of therapeutic and double therapeutic dose of ivermectin on mature male rabbits aswell as effect of therapeutic dose of same drug on pregnant female rabbits. Forty mature rabbits were used inthis study (30 males and 10 pregnant female). Male rabbits received therapeutic and double therapeutic dosesof ivermectin, while female rabbits received therapeutic dose. The histopathological results revealed mild tomoderate reversible degenerative changes in examined organs of male rabbits received ivermectin weekly for4weeks meanwhile, this degeneration become more severe and extended to complete necrosis in some organsafter longer period and higher doses of ivermectin administration. As well as, pregnant females suffered fromabortion in some cases with fetal death. We concluded that ivermectin has adverse effect on male rabbits thatreceived either therapeutic or double therapeutic doses that varied from mild degenerative changes to completenecrosis of spermatogenic cells with complete absence of sperms. Meanwhile, female genital system wasseverely affected that showing severe degeneration and hemorrhage in uterus as well as atritic follicles anddegenerated ova in ovaries. So, caution must be considered for ivermectin administration especially duringbreeding season and for pregnant females.
https://bvmj.journals.ekb.eg/article_31162_fece43fcb7398561c3fe9204335037fc.pdf
2017-03-01
104
112
10.21608/bvmj.2017.31162
Ivermectin
Pathology
Male rabbits
pregnant female rabbits
Mahmoud
GabAllh
1
Pathology department, faculty of vet. Med., Benha University
AUTHOR
AbdEl-baset
El-mashad
2
Pathology department, faculty of vet. Med., Benha University
AUTHOR
Aziza
Amin
3
Pathology department, faculty of vet. Med., Benha University
AUTHOR
Marwa
Darweish
4
Pathology department, faculty of vet. Med., Benha University
AUTHOR
ORIGINAL_ARTICLE
Mycotic quality of locally manufactured smoked herring fish
A grand total of 90 random samples of smoked herring fishes (30 Un packed –30 packed – 30 fillet) were collected fromdifferent markets at Monofia governorate. The samples were subjected to mycological examination for count, isolationand identification of mould and yeast. Also Molecular identification of aflatoxinogenic A. flavus was carried out. Theincidence of mould in the examined samples (unpacked, packed and fillet) were 100%, 83.3% and 66.7%, respectively.While the incidence of yeast in the examined samples (unpacked, packed and fillet) were 90%, 66.7% and 33.3%,respectively. The mean total mould counts /g was 1.9×105, 2.1×104, 2.5×103 for unpacked, packed and fillets, respectively.The mean total yeast counts/g was1.7×105, 2.1×104,1.3×102, respectively. The results indicated that un packed smokedherring fish were more contaminated with mould and yeast. Molecular identification of aflatoxinogenic A. Flavus waspositive for three sets of different virulence genes (omtA, ver-1, and nor-1) which involved in aflatoxin biosyntheticpathway.
https://bvmj.journals.ekb.eg/article_31165_c3c06a42126f5132c3219980f92f7819.pdf
2017-03-01
113
120
10.21608/bvmj.2017.31165
Smoked herring
Mycotoxins
Aflatoxin biosynthesis
Aspergillus flavus
Hemmat
Ibrahim
drhemmat01@yahoo.com
1
Department of food Hygiene, Fac. Vet. Med, Benha University
AUTHOR
Rasha
El Sabagh
2
Department of food Hygiene, Fac. Vet. Med, Benha University
AUTHOR
Nahla
Abou El-Roos
3
Food Hygiene Dept., Animal Health Research Institute Shebin El- Kom branch
AUTHOR
Abd El kareem
Mohebat
4
Food Hygiene Dept., Animal Health Research Institute Shebin El- Kom branch
AUTHOR
ORIGINAL_ARTICLE
Comparative studies of Listeria monocytogenes survival in bifidus and traditional yoghurt during production and storage
Traditional yoghurt (TY) (Streptococcus thermophiles+ Lactobacillus bulgaricus) and Bifidus yoghurt (BY) samples(Streptococcus thermophiles+ Lactobacillus bulgaricus+ Bifidobacterium bifidum). Traditional & Bifidus yoghurtsamples were inoculated with 106 cfu/g L. monocytogenes and stored for 21 days at 4±1oC. Samples were taken at zerotime (fresh samples) then after 3, 5, 7, 10, 14 and 21 days of storage for titratable acidity measurement and bacterial countof the culture starters and L. monocytogenes counts. The results revealed that the titratable acidity of TY & BY wasincreased during the 21 days of storage, while the population of L. monocytogenes became non-detectable level in 10thdays of storage in BY and still detected in TY till the day 14th of storage and became 2.20± 0.31 log10 cfu/g. The countof all starter cultures (S. thermophiles+ Lb. bulgaricus+ B. bifidum) in both yoghurts increased during the fermentationand cold storage and remained stable with values >6 log10 cfu/g throughout the storage period at 4±1°C.
https://bvmj.journals.ekb.eg/article_31168_acbc4ef04d67c21fa70ae9e4c594dc30.pdf
2017-03-01
121
126
10.21608/bvmj.2017.31168
probiotics
Bifidobacteria
L. monocytogenes
yoghurt
bifidus yoghurt
Huda
Othman
1
Food Hygiene Department, Animal Health Research Institute, ARC, Dokki, Giza, Egypt
AUTHOR
Hamdi
Abdelsamei
2
Milk Hygiene, Department of Food Control, Fac. of Vet. Med., Benha Univ., Egypt
AUTHOR
Adham
Abdou
3
Milk Hygiene, Department of Food Control, Fac. of Vet. Med., Benha Univ., Egypt
AUTHOR
Ekbal
Ibrahim
iqbal.abdo@fvtm.bu.edu.eg
4
Milk Hygiene, Department of Food Control, Fac. of Vet. Med., Benha Univ., Egypt
AUTHOR
Azza
Elbaba
5
Food Hygiene Department, Animal Health Research Institute, ARC, Dokki, Giza, Egypt
AUTHOR
ORIGINAL_ARTICLE
Evaluation the effect of Lactobacillus acidophilus probiotic culture over Staphylococcus aureus during the production and storage of acidophilus yoghurt
The survival of Staphylococcus aureus in the acidophilus yoghurt (AY) was evaluated during the production and coldstorage. 21 acidophilus yoghurt samples were prepared with traditional yoghurt culture (Streptococcus thermophiles &Lactobacillus bulgaricus) with Lactobacillus acidophilus and inoculated with 106 cfu/ml S. aureus and stored for 21 daysat 4oC. Samples were taken at zero time (fresh samples), 3, 5, 7, 10, 14 and 21 days for titratable acidity, culture starters’counts and S. aureus count. The results revealed that the titratable acidity% of AY was increased and reached 0.72, 0.78,0.83, 0.87, 0.89, 1.02 and 1.45% at zero time, 3, 5, 7, 10, 14, and 21 days of storage, respectively, while the population of S. aureus reached 6.10 and 3.70 log10cfu/g at zero time and 3rd day of storage, respectively and became non-detectable level in 5th days of storage. The count of all starter cultures (S. thermophiles+ L. bulgaricus+ L. acidophilus) in acidophilus yoghurt increased during the fermentation and cold storage, S. thermophiles became 7.15, 7.35, 8.20, 8.15, 7.45, 7.45 and 6.65 log10cfu/g while L. bulgaricus 7.25, 7.55, 8.25, 8.73, 7.76, 7.47 and 6.54 log10cfu/g and L. acidophilus became 7.38, 7.70, 8.25, 8.20, 7.50, 7,46 and 6.66 log10cfu/g at zero time, 3, 5, 7, 10, 14, and 21 days of storage, respectively. It concluded that S. aureus count decreased gradually during the production and cold storage and became non-detectable level on the 5th days of cold storage. The survival of all starter cultures in yoghurt sample remained stable with values > 6 log10 cfu/g throughout the storage period at 4±1°C.
https://bvmj.journals.ekb.eg/article_31197_912b2397225a1d92979c86befdadac05.pdf
2017-03-01
127
131
10.21608/bvmj.2017.31197
probiotic
Lactobacillus acidophilus
S. aureus
yoghurt
acidophilus yoghurt
Huda
Othman
1
Food Hygiene Department, Animal Health Research Institute, ARC, Dokki, Giza, Egypt
AUTHOR
Azza
Elbaba
2
Food Hygiene Department, Animal Health Research Institute, ARC, Dokki, Giza, Egypt
AUTHOR
Hamdi
Abdelsamei
3
Milk Hygiene, Department of Food Control, Fac. of Vet. Med., Benha Univ., Egypt
AUTHOR
Adham
Abdou
4
Milk Hygiene, Department of Food Control, Fac. of Vet. Med., Benha Univ., Egypt
AUTHOR
Ekbal
Ibrahim
iqbal.abdo@fvtm.bu.edu.eg
5
Milk Hygiene, Department of Food Control, Fac. of Vet. Med., Benha Univ., Egypt
AUTHOR
ORIGINAL_ARTICLE
Characterization of Methicillin Resistance Staphylococcus aureus isolated from chicken and human
Staphylococcus aureus causes a wide range of diseases in human beings, from minor skin infections to severe illnessessuch as septicemia, toxic shock, endocarditis, and pneumonia. Staphylococcus aureus is a major pathogen of increasingimportance due to the rise in antibiotic resistance. S. aureus was an important cause of disease in poultry it could beinvolved in a wide range of clinical conditions such as septicemia, bone and Joint infections, abscesses and dermatitis.Inthis study, 160 samples were collected , out of which 80 samples were from poultry and human patients (80 for each ).The incidence of infection with S.aureus in Bumble foot, Skin swabs in chicken and Diabetic foot swab , Skin sawb andNasal swab in human were, 10%, 66%, 30%, 40%, 67.5% respectively. Methicillin resistant S. aureus (MRSA) wereincidence in chicken and human 66.6% and 33.3% respectively. Subjected to PCR for detection of some antimicrobialresistance genes using intrinsic methicillin resistant gene (mec A), the IgG binding region of protein A( spa) gene were83.3% and 50 % respectively also beta-lactamase (blaZ) gene 100% and 100% respectively were detected in most MRSAisolates either from human or chicken samples
https://bvmj.journals.ekb.eg/article_31198_3fc232b9e8fa9807b3bd62e8c24637f9.pdf
2017-03-01
132
137
10.21608/bvmj.2017.31198
Methicillin resistant – S.aureus – human – chicken – mec A gene – blaZ gene –spa gene
Ashraf
Abd El-Tawab
1
Bacteriology, Immunology and Mycology Dep., Fac. of Vet. Med., Benha Univ.
AUTHOR
Fatma
Hofy
2
Bacteriology, Immunology and Mycology Dep., Fac. of Vet. Med., Benha Univ.
AUTHOR
Sahar
Mohamed
3
Bacteriology Department , Animal Health Research Institue, Doki , Giza.
AUTHOR
Sohila
Amin
4
Bacteriology Department , Animal Health Research Institue, Doki , Giza.
AUTHOR
ORIGINAL_ARTICLE
HACCP application during different processing stages of beef fillet with special references to corrective action
Here, in this study HACCP program was applied for the production of beef fillet in an Egyptian hotel and the microbialload before and after application was compared. The aerobic plate count monitored before and after application ofcorrective action during different successive production stages of beef fillet in tested hotel to examine the frequency ofcontamination. The mean values of Aerobic plate count before application of corrective actions were 20.47 ± 7.01, 71.33± 15.27, 680.0 ± 385.53, 528.67 ±255.39, 24.33 ± 8.09, and 24.33 ± 8.09 (CFU/gm) for the examined frozen, thawed,formed, salted, cooked and served meat, respectively. After application of corrective action, the aerobic plate countdeclined to be 13.80 ± 4.20, 48.00 ± 7.32, 118.67± 26.97, 92.67 ± 20.34, 7.13 ± 1.95, and <10 in the examined frozen,thawed, formed, salted, cooked and served meat, respectively. The reduction percentages for the above examined sampleswere 32.5%, 32.7, %82.5%, 82.4%, and 70.6% and 100%, respectively. It is noticed that meat meals can be contaminatedby several ways such as incorrect thawing, inadequate cleaning and sanitation for utensils or cross contamination aftercooking resulting in higher contamination with microorganisms. No food borne pathogens of public health hazardsdetected during this study. This results shows the significant importance of HACCP program for prevention and controlof pathogenic and spoilage microorganisms.
https://bvmj.journals.ekb.eg/article_31199_ccf58cfba2bb6ee5aa5062e135c2ba57.pdf
2017-03-01
138
144
10.21608/bvmj.2017.31199
HACCP
aerobic plate count
beef fillet
Salmonella
E. coli
Khalid
Abukady
1
Food hygiene Dept., Faculty of Veterinary Medicine, Benha University
AUTHOR
Fahim
Shaltout
2
Food hygiene Dept., Faculty of Veterinary Medicine, Benha University
AUTHOR
Reham
Amin
rehamnour2007@yahoo.com
3
Food hygiene Dept., Faculty of Veterinary Medicine, Benha University
AUTHOR
Muhammad
El-Shatter
4
Animal Health Research National Institute, Food Control Dept., El-Dokki –Giza
AUTHOR
Islam
Sabike
5
Food hygiene Dept., Faculty of Veterinary Medicine, Benha University
AUTHOR
ORIGINAL_ARTICLE
Food hygiene Dept., Faculty of Veterinary Medicine, Benha University
The present study was performed on a total of 124 milk samples from small scale producers, farmers and markets at El-Menofyia Governorate. The prevalence of Streptococcus species in the examined samples was (65.3%), where S.agalctiae, S. dysgalactiae, S. uberis, S. pyogenes and S. pneumoniae were 28.2%, 11.3%,16.1%, 8.9% and 0.8%;respectively. The antibiogram for Streptococcus spp. revealed that vancomycin and erythromycin were the most properantibiotics with the highest efficiency against isolated Streptococcus spp., but they were resistant to cefatriaxone andchlormphenicol. Additionally, S. agalactiae and S. dysgalactiae were sensitive to penicillin, ofloxacin ;respctively.However, S. uberis was sensitive to amoxicillin and clindamycin. By using PCR, virulence gene hyalurinidase (hyl) wasdetected in 25% of S. agalactiae, while a surface expressed M-like protein (mig) gene was detected in 100% of S.dysgalactiae. Also plasminogen activator (pauA) gene was detected in 100% of S. uberis isolates
https://bvmj.journals.ekb.eg/article_31201_8908403a9070c15ee297a4b48b98b59b.pdf
2017-03-01
145
152
10.21608/bvmj.2017.31201
Streptococcus
Raw milk
virulence gene
PCR
Ashraf
AbdEL-Tawab
1
Bacteriology, Immunology and Mycology Dep., Fac. Vet. Med. Benha Univ.
AUTHOR
Fatma
El-Hofy
2
Bacteriology, Immunology and Mycology Dep., Fac. Vet. Med. Benha Univ.
AUTHOR
Nahla
Abou El-Roos
3
Animal Health Research “Shebin El- Kom branch”
AUTHOR
Hassnaa
Abdullah
4
Menofiya Vet. Med. Organization, Shebin El-kom
AUTHOR
ORIGINAL_ARTICLE
Environmental Factors Affecting Some Productive and reproductive Traits in Egyptian Buffaloes
This study was conducted to evaluate the effect of some environmental factors on milk production in Egyptian buffaloesand the effect of parity and season of calving on calving interval and days open. There was a significant effect of parity(P ≤ 0.05) on 305day milk yield, while season of calving had a highly significant effect (P ≤ 0.01) on 305day milk yield.Animals with age at first service less than 21 months and age at first calving less than 35 months had higher milk yieldthan other animals (2489.5 and 2526.7 kg, respectively). With increasing days open, milk production increased so animalshad more than 160 days open produced 2757.8 kg milk. Milk yield in winter (2600.5 kg) was higher than in summerseason (2441.5 kg). Animals which conceived four or more services produce more milk yield (2529.8 kg). Season ofcalving had a significant effect (P ≤ 0.05) but parity had a highly significant effect (P ≤ 0.01) on calving interval and daysopen. There was direct relationship between milk yield with each of calving interval and days open. Animals that gavemore than 3000 kg showed the maximum calving interval and days open (14 months and 111.8 days, respectively)
https://bvmj.journals.ekb.eg/article_31202_f2e7e909f5b9c81cc304b21fca3cec07.pdf
2017-03-01
153
159
10.21608/bvmj.2017.31202
calving interval
305day milk yield
Egyptian Buffaloes
Paritym Season of calving
Services
Basant
ShafiK
1
Animal Wealth Development Dept., Fac. of Vet. Med., Benha Univ.
AUTHOR
El-Bayomi
Kh.
2
Animal Wealth Development Dept., Fac. of Vet. Med., Zagazig Univ.
AUTHOR
Abo-Salem,
S.
3
Forensic Medicine Dept., Fac. of Vet. Med., Benha Univ.
AUTHOR
Darwish
A.
4
Animal Production Research Institute, ARC., Ministry of Agriculture, Doki, Giza, Egypt
AUTHOR
ORIGINAL_ARTICLE
Detection of SNPs in Exon10 Locus of FSHR Gene and Their Association with Some Infertility problems in Egyptian Buffaloes
Follicle-stimulating hormone (FSH) is a glycoprotein hormone. It helps in development and maturation of thefollicles in females through binding to FSH receptors which consists of 10 exons and 9 introns. The objectiveof this study was to estimate some genetic factors affecting milk production and fertility traits and to detectpolymorphisms in exon 10 of FSHR gene and determine the associations between these polymorphisms andinfertility in Egyptian buffalo. Heritability estimates were (0.19, 0.18, 0.07, 0.10, 0.21 and 0.19) for calvinginterval, days open, dry period, days in milk, total milk yield and 305day milk yield (305 DMY), respectively.Dry period showed high positive phenotypic and genetic correlation with day's open and calving interval. Thebreeding value for 305 DMY ranged from -480 to 380, from -370 to 330 and between -230 to 170 kg for cow,sire and dam, respectively. There was one non synonymous SNP (A93G) at 93bp in exon 10 of FSHR gene(with 230bp size) and there was two different pattern of single strand conformation polymorphism (SSCP) butthere was no any SNP for FSHR gene (with 306bp size) in all examined Egyptian buffaloes as shown by oneSSCP pattern and nucleotide sequencing.
https://bvmj.journals.ekb.eg/article_31203_03553a06d15969ae986e92f078371a60.pdf
2017-03-01
160
169
10.21608/bvmj.2017.31203
correlation. Egyptian buffaloes. FSHR. heritability. Polymorphism. SNP. SSCP
Basant
ShafiK
1
Animal Wealth Development Dept., Fac. of Vet. Med., Benha Univ.
AUTHOR
El-Bayomi
Kh.
2
Animal Wealth Development Dept., Fac. of Vet. Med., Zagazig Univ.
AUTHOR
Abo-Salem
S.
3
Animal Wealth Development Dept., Fac. of Vet. Med., Zagazig Univ.
AUTHOR
Darwish
A.
4
Animal Production Research Institute, ARC., Ministry of Agriculture, Doki, Giza, Egypt
AUTHOR
Sherif
Ramadan
sherif.ramadan@fvtm.bu.edu.eg
5
Animal Wealth Development Dept., Fac. of Vet. Med., Benha Univ.
AUTHOR
ORIGINAL_ARTICLE
Environmental Contamination with Methicillin Resistant Staphylococci in Food animal carcasses
One hundred random samples of cattle and sheep meat, equipment and workers hands swabs (25 samples of each) werecollected from different abattoirs in Kalyobia governorates. The obtained results indicated that the mean values of APC,and S. aureus counts in the examined samples were 1.5 x 104 ± 0.9 x 104, 1.77 x 103 ± 1 x 103CFU /g for cattle meat, 1 x104 ± 0.65 x 104, 1.57 x 105 ± 0.83 x 105 CFU /g for sheep meat, 7.4 x 103 ± 0.29 x 104, 1.1 x 103 ± 0.33 x 103 CFU /g forequipment swab and 5.77 x 103 ± 0.3 x 104, 7.4 x 103 ± 0.49 x 104CFU /g, for workers hands swab, respectively. Theincidence of S. aureus was 52%, 48%, 56% and 64% in the examined samples of cattle and sheep meat, equipments andworkers hands swabs, respectively. On the other hand, Methicillin Resistant S. aureus (MRSA) was isolated from 32%,24%, 40% and 44% of the examined samples of cattle and sheep meat, equipments and workers hands swab respectively.The significance of the isolated bacteria in the examined animal carcasses at abattoir level and possible sources ofcontamination as well as some recommendations to improve the quality of these carcasses were discussed
https://bvmj.journals.ekb.eg/article_31204_684d8975a3a3fe003acee5f5e5a95d79.pdf
2017-03-01
170
176
10.21608/bvmj.2017.31204
meat
abattoir
S. aureus
MRSA
carcass
Amani
Salem
amani.salem@fvtm.bu.edu.eg
1
Faculty of Veterinary Medicine, Benha University, Egypt
AUTHOR
Abd El Raheem
A.
2
Faculty of Veterinary Medicine, Benha University, Egypt
AUTHOR
Zakaria
M.
3
Animal Health Research Institute, Dokki, Egypt
AUTHOR
ORIGINAL_ARTICLE
Efficiency of some essential oils in Control of Methicillin Resistant Staphylococcus Aureus (MRSA) in Minced Beef
In this study, the antibacterial effect of Cinnamon, Thyme and Lemongrass essential oils against Methicillin ResistantStaphylococcus aureus (MRSA) artificially inoculated into refrigerated minced beef was investigated. Sensory analysisindicated significant advantages in using these oils in refrigerated minced beef. Also, results indicated that the MRSAcounts decreased as the concentration of the oil increases since the concentration (1.5%) gives the best effectiveness. Inaddition, a highly significant differences (P<0.05) as a result of oil treatments were noticed. The antibacterial activitiesof the added essential oils followed the order, Cinnamon > Thyme > Lemongrass oil. The shelf life of the treated sampleswas extended than control ones. In conclusion, these oils especially cinnamon one can be useful for commercialapplication as potential food preservatives and anti- MRSA agents in food
https://bvmj.journals.ekb.eg/article_31205_809b0abccb5c5f65bf473af8ba00d052.pdf
2017-03-01
177
183
10.21608/bvmj.2017.31205
MRSA
Essential oils
minced beef
Antimicrobial
Amani
Salem
amani.salem@fvtm.bu.edu.eg
1
Faculty of Veterinary Medicine, Benha University, Egypt
AUTHOR
Abd El -Raheem
A.
2
Faculty of Veterinary Medicine, Benha University, Egypt
AUTHOR
Zakaria
M.
3
Animal Health Research Institute, Dokki, Egypt
AUTHOR
ORIGINAL_ARTICLE
Prevalence of eaeA and qacEΔ1 genes in Escherichia coli isolated from omphalitis in baby chicks
A total of two hundred diseased Saso chicks with omphalitis were examined (1400 samples) for the isolation of E. colifrom different organs (liver, caecum, spleen, heart, lung, yolk sac and cloacal swab). Results showed that 64 cases werepositive with an incidence of 32%. Fifty isolates (25%) of E. coli were recovered from chicks could be sero-grouped in19 O groups with the most predominant serotype was O91 20 % (10 out of 50 isolates), O26 10% (5 strains out of 50) O788% (4 strains out of 50), and 7 strains untypable E. coli 14%. Among E. Coli O groups were found to be resistant toAmoxicillin 93.02% followed by Tetracycline 74.42%, Enrofloxacin 46.51%, Erythromycin 30.32%, Ciprofloxacin27.91%, Norofloxacin and Streptomycin 20.93% and Gentamycin 6.98%. While they were found to be sensitive forGentamycin, Streptomycin, Ciprofloxacin, Tetracycline, Erythromycin, Norofloxacin, Enrofloxacin, and Amoxicillin asthe following: 88.37%, 46. 51%, 34.89%, 23.26%, 18.60%, 9.30%, 9.30% and 6.98%, respectively. The incidence rateof eaeA gene of E. coli was 15.79%. Quaternnary ammonium compound (qacEΔ1) gene also was detected in E. coli withincidence rate 63.16%.
https://bvmj.journals.ekb.eg/article_31206_f2aeaa13f19f20e0070b433695be00a5.pdf
2017-03-01
184
192
10.21608/bvmj.2017.31206
Omphalitis
E. coli
virulence gene
Antibiotic resistance
Disinfectant resistant gene
Ashraf
Abd El- Tawab
1
Bacteriology, Immunology and Mycology Department, Faculty of Veterinary Medicine, Benha University
AUTHOR
Fatma
El- Hofy
2
Bacteriology, Immunology and Mycology Department, Faculty of Veterinary Medicine, Benha University
AUTHOR
Soad
Nasef
3
Animal Health Research Institute, Dokki Lab., Giza
AUTHOR
Ola
Ibrahim
4
Animal Health Research Institute, Dakahlia branch
AUTHOR
ORIGINAL_ARTICLE
Isolation of Aphanomyces invadans Associated with Skin Lesions in African Catfish" Clarias gariepinus"
The present study aimed to isolate and identify of Aphanomyces invadans from naturally infected African Catfish" Clariasgariepinus". A total number of 105 cultured Clarias gariepinus collected from private fish farms in Kafr Al-Sheikh &Behera Governorates. Result revealed that the prevalence of infection among the examined fish was 8.6 % and the diseasemostly recorded during winter and spring. The infected fish developed characteristic ulcerative lesions and fin rot.Fungus-like oomycetes Aphanomyces invadans grow on glucose peptone yeast (GPY) agar as opaque colonies withuneven white transparent velvets surface at room temperature. Microscopically, lactophenol cotton blue stained fungalgrowth appeared as non-septated thin long branched hyphae with tapered end containing cytoplasmic organelles. Onsporulating media, the rectangular shaped spores appeared inside the hyphae connected together by thin filament. Inconclusions, EUS is an invasive disease of Clarias gariepinus and care should be taken with low temperature in managingfish pond.
https://bvmj.journals.ekb.eg/article_31207_3dc3eb8e8130befe3663c3bf390e29c5.pdf
2017-03-01
193
197
10.21608/bvmj.2017.31207
Aphanomyces invadans
Clarias gariepinus
identification
prevalence
sporulation
Hadeer
Youssuf
1
Dept. of Fish Diseases and Mangement, Fac. of Vet. Med., Benha Univ., Egypt
AUTHOR
Eman
Abdel Gwad
2
Dept. of Fish Diseases and Mangement, Fac. of Vet. Med., Benha Univ., Egypt
AUTHOR
Amel
El-Asely
3
Dept. of Fish Diseases and Mangement, Fac. of Vet. Med., Benha Univ., Egypt
AUTHOR
Hiam
Elabd
4
Dept. of Fish Diseases and Mangement, Fac. of Vet. Med., Benha Univ., Egypt
AUTHOR
AA
Shaheen
5
Dept. of Fish Diseases and Mangement, Fac. of Vet. Med., Benha Univ., Egypt
AUTHOR
Amany
Abbass
amanyabbass3@yahoo.com
6
Dept. of Fish Diseases and Mangement, Fac. of Vet. Med., Benha Univ., Egypt
LEAD_AUTHOR
ORIGINAL_ARTICLE
Incidence and antibiogram of Clostridium perfringens isolated from herbs and spices widely distributed in the Egyptian market
The present study was designed for isolation and identification of Clostridium perfringens (C. perfringens) from herbsand spices that are widely distributed in the Egyptian markets; and determination of the drug of choice for its control basedon the Antimicrobial Susceptibility Test (AST). A total of 392 samples of herbs and spices were randomly andanonymously selected from the enormous number of samples obtained from the Egyptian markets. An overall occurrenceof C. perfringens was 8.42% (33 out of 392). Separately, occurrence was higher in herbs than spices being 10.5 and 6.25%,respectively. C. perfringens isolates were identified and confirmed with macroscopical, microscopical and biochemicalanalysis. AST results revealed that isolates of C. perfringens from the tested herbs and spices samples were found mostsusceptible to ampicillin-sulbactam and Penicillin-G with inhibition zones of 19.4 ± 0.98 and 16.6 ± 1.16 mm, respectively.On the other hand, the isolates were found nearly resistant to clindamycin and vancomycin with inhibition zones of 6.28± 0.63 and 8.78 ± 0.41 mm, respectively. Meanwhile, the isolates were intermediary susceptible to trimethoprimsulphamethoxazolecombination and chloramphenicol. Data of the present study indicate occurrence of C. perfringens inherbs and spices with a considerable rate and the antimicrobials of choice for its control are penicillins and ceftazidime.higher sanitation procedures in herbs and spices industry before its distribution to the Egyptian markets have beenrecommended
https://bvmj.journals.ekb.eg/article_31208_4bc9e8ef0333be661d56e01a9f10a727.pdf
2017-03-01
198
206
10.21608/bvmj.2017.31208
C. perfringens
Incidence
prevalence
herbs
Spices
AST
Ashraf
Abd El-Tawab
1
Department of Microbiology, Faculty of Veterinary Medicine, Benha University, 13736 Moshtohor, EGYPT
AUTHOR
Mohamed
Abdallah
2
Department of Microbiology, Central Lab of Residue Analysis of Pesticides & Heavy Metals in Food, Agricultural Research Center, Ministry of Agriculture
AUTHOR
Hend
Yusuf
dr.hendyusuf@gmail.com
3
Department of Microbiology, Central Lab of Residue Analysis of Pesticides & Heavy Metals in Food, Agricultural Research Center, Ministry of Agriculture
LEAD_AUTHOR