ORIGINAL_ARTICLE
Bacteriological and molecular detection of brucellosis with special reference to the effect of disinfectants on isolated strains
A total of 127 specimens (13 aborted foeti, 46 milk samples, 37 lymph nodes, 14 livers, 14 spleen and 6 vaginaldischarges) were collected and examined for isolation and typing of Brucella microorganism. The resultsdetected 15 strains (5 aborted foeti, 4 milk, 5 lymph nodes and 1 spleen) were detected and typed as Br.melitensis biovar 3. Application of PCR test for rapid identification of Brucella strains which isolated fromlymph nodes five of naturally infected animals (two cattle, one buffaloes, one sheep and one goat) revealedthat all samples were reacted positively with Br. melitensis specific DNA products with a molecular size of731 pb. On sequencing, the Nucleotide sequence alignment of obtained sequences with other Brucella strainindicated that the obtained isolate have high identity with Br. melitensis biovar 3. The bacteriocidal activity oftested disinfectants against isolated Br. melitensis strain at variables concentration revealed that halogenshowed highest bactericidal activity followed by QACs and phenolic while alkaline wasthe lowest effect
https://bvmj.journals.ekb.eg/article_31210_c400a92610ce868e75654b9d6ccc2710.pdf
2016-09-01
1
9
10.21608/bvmj.2016.31210
Brucellosis
isolation
PCR
disinfectants sensitivity
Lobna
Salem
lobna.khamis@fvtm.bu.edu.eg
1
Department of Zoonoses, Faculty of Vet. Med., Benha University
AUTHOR
Nashwa
Khalifa
2
Department of Zoonoses, Faculty of Vet. Med., Benha University
AUTHOR
Khoudair
M.
3
Brucella Research Department, Animal Health Research Institute, Dokki
AUTHOR
Samar
Moustafa
samar.mustafa@fvtm.bu.edu.eg
4
Department of Zoonoses, Faculty of Vet. Med., Benha University
AUTHOR
ORIGINAL_ARTICLE
Sero-diagnosis of brucellosis in Gharbiya governorate, Egypt
A total 1006 animals (300 cattle, 300 buffaloes, and 300 sheep and106 goats) were selected from private farms andsuspected to suffer from brucellosis from different localities in Gharbiya governorate, as well as 50 rats (31Rattus rattus&19 Rattus norvegicus) and 15 stray dogs were collected from the same localities associated with examined animals. Inaddition, 160 persons suffering from fever suspected to be brucellosis were collected (80 workers contact with examinedanimals and 40 from fever hospitals). Serological tests were carried out by using Rose Bengal plate (RBPT), BufferedAcidified plate test (BAPAT), Complement Fixation test (CFT), Tube Agglutination test (TAT) and 2- Mercapto-Ethanoltest (2-MET). The results showed that the percentage of positive reactors were 9%, 7.3%, 9.3% 8.5%, 8% and 0% usingRBPT in cows, buffaloes, sheep, goats, rats and dogs respectively. Meanwhile the percentage of positive reactors usingBAPAT was 9.6%, 8.3%, 10.7%, and 9.6% in cows, buffaloes, sheep and goats and by using CFT the percentage was9.3%, 8%, 10.3% and 10.3% in previously examined animals. Also the result of TAT was 8% in rats and 0.0% in dogs.The occurrence of brucellosis in sheep and goats was higher than cows and buffaloes. Finally, the results in humans were13.1%, 11.3% and 10% by using RBPT, TAT and 2MET respectively. The incidence of brucellosis was higher in males(14.9%) than females (3.8%) and higher in humans aged between 20-30 years
https://bvmj.journals.ekb.eg/article_31211_2f911ab10579dfeb8584ddd24616fc14.pdf
2016-09-01
10
16
10.21608/bvmj.2016.31211
Brucellosis
serology
reservoirs
Egypt
Lobna
Salem
lobna.khamis@fvtm.bu.edu.eg
1
Department of Zoonoses, Faculty of Vet. Med., Benha University
AUTHOR
Nashwa
Khalifa
2
Department of Zoonoses, Faculty of Vet. Med., Benha University
AUTHOR
Khoudair
M.
3
Brucella Research Department, Animal Health Research Institute, Dokki
AUTHOR
Samar
Moustafa
samar.mustafa@fvtm.bu.edu.eg
4
Department of Zoonoses, Faculty of Vet. Med., Benha University
AUTHOR
ORIGINAL_ARTICLE
Effect of rosemary and green tea extracts on overall quality and shelf-life of meat during storage
The present study was designed to investigate antimicrobial and antioxidant activity of green tea and rosemary extractson minced meat samples. Minced meat was divided into 2 groups, untreated (control) and treated samples. The treatedsamples homogenized with green tea extract (700 ppm), green tea extract (1000 ppm), rosemary extract (0.2%), mixtureof green tea extract (500 ppm) & rosemary extract (0.2%), and mixture of green tea extract (500 ppm) & rosemaryextract (0.3%). The minced meat samples were examined physically, bacteriologically and chemically to determine theirkeeping quality by counting aerobic plate, Staphylococcal and Enterobacteriacae counts, as well as measuring pH, TBAand TVN immediately after preparation (zero time) to 6 days of chill storage (4±1°C). Green tea (1000 ppm) group givethe best effectiveness with a significant advantage in extend shelf-life of refrigerated minced meat to 6 days compared toall groups specially control ones (3 days).
https://bvmj.journals.ekb.eg/article_31212_24d74551e329e4c18df30924c9ba107b.pdf
2016-09-01
17
23
10.21608/bvmj.2016.31212
Antimicrobial
antioxidant
green tea
rosemary
Amani
Salem
amani.salem@fvtm.bu.edu.eg
1
Food Control Dept., Faculty of Veterinary Medicine, Benha University, Egypt
AUTHOR
Dalia
Khater
2
Animal Health Research Institute., Department of Food Hygiene., Tanta branch
AUTHOR
Shimaa
El Barody
3
Animal Health Research Institute., Department of Food Hygiene., Tanta branch
AUTHOR
ORIGINAL_ARTICLE
Bacteriological Evaluation of Some Fresh and Frozen fish
Ninety random samples of fresh and frozen each of Tilapia niloticus, Mugil cephalus and Shrimp (15 of each) werecollected from different fish markets at Qaliuobia Governorate. All collected samples were bacteriologically examinedfor determination of Aerobic Plate Count (APC), psychrotrophic count, Isolation and Identification of pseudomonas andAeromonas species. The bacteriological examination revealed the mean values of APC in the examined fish samples was1.52×106 ± 0.47×106 and 3.08×105 ± 1.31×105 in fresh and frozen Tilapia niloticus, 7.14×105 ± 1.84×105 and 2.59×105 ±0.62×105 in fresh and frozen Mugil cephalus and 7.81×104 ± 2.07×104 and 1.36×104 ± 0.42×104/g, in fresh and frozenshrimp . The mean values of psychrotrophic count in fresh and frozen Tilapia niloticus were 2.67×105 ± 0.45×105 and5.36×105 ± 1.24×105 cfu/g, respectively. 4.91×104 ± 0.93×105 and 1.72×105 ± 0.46×105/ gm respectively in fresh andfrozen Mugil cephalus. 7.81×104 ± 2.07×104 and 6.51×104 ± 1.80×104/g, in fresh and frozen shrimp. The incidence ofPseudomonas species was 33.3% and 53.3%in the examined samples of fresh and frozen Tilapia niloticus, 46.7% and60.0 %in fresh and frozen Mugil cephalu, respectively, 33.3 %and 40.0 %in fresh and frozen shrimp, respectively. Theincidence of Aeromonas species were 46.7 %and 33.3 %in the examined samples of fresh and frozen Tilapia niloticus,53.3 % and 40.0% in fresh and frozen Mugil cephalus, respectively, 33.3 % and 40.0 %in fresh and frozen shrimp,respectively
https://bvmj.journals.ekb.eg/article_31213_5d2725bfdcd86464ec7d8ce676a88f1d.pdf
2016-09-01
24
29
10.21608/bvmj.2016.31213
Fresh fish
frozen fish
Tilapia niloticus
Mugil cephalus
Hemmat
Ibrahim
drhemmat01@yahoo.com
1
Food control Dept., Fac. Vet. Med.
AUTHOR
Reham
Amin
rehamnour2007@yahoo.com
2
Food control Dept., Fac. Vet. Med.
AUTHOR
Nahela
Shawkey
3
Animal Research Institute Shebin El- Koom
AUTHOR
Hend
Mohammed
4
Animal Research Institute Shebin El- Koom
AUTHOR
ORIGINAL_ARTICLE
Enterotoxin producing S. aureus in salted fish
A total of 90 samples of sardine, molouha and feseikh (30 of each) were collected from different retail markets forbacteriological and molecular examination. The average of Staphylococci counts (cfu/g) were ranged from1.0 x 102 to1.1x104 in sardine, ranged from 1.0x102 to 3.4×105 in molouha and 1.0×102 to 7.8×105 in feseikh. with a mean value2.75×103 ± 0.41×103, 1.98×104 ± 0.28×104 and 5.03×104 ± 1.12×104, respectively. Concerning to S. aureus it was detectedin 36.67%, 46.67% and 50.00% of the examined salted fish sardine, molouha and fesiekh, respectively. Totally 44.44%of the examined samples of salted fish were contaminated with S. aureus. The incidence of enterotoxins (A, B, C and D)produced by S. aureus were 20.00%, 40.00%, 60% of sardine, molouha and fesiekh. Modern rapid methods as PolymeraseChain Reaction (PCR) has high sensitivity, specificity and reduce detection time. It offers advantages over conventionaldiagnostic methods
https://bvmj.journals.ekb.eg/article_31214_9a58d4aa18673029d827c26a2ab9a8a8.pdf
2016-09-01
30
34
10.21608/bvmj.2016.31214
salted fish
Staph. aureus
enterotoxin
PCR
Faten
Hassanien
1
Food Control Dept., Fac. Vet. Med. Benha University
AUTHOR
M.
Hassan
2
Food Control Dept., Fac. Vet. Med. Benha University
AUTHOR
Nahla
Shawkey
3
Animal Health Research Institute, Shibin EI-KOOM
AUTHOR
Ahmed
A.
4
Animal Health Research Institute, Shibin EI-KOOM
AUTHOR
ORIGINAL_ARTICLE
Impact of some natural preservatives on Bacterial Profile of Minced Meat in Egypt
In this study, the antioxidant and antibacterial effect of Marjoram and Cumin oils (0.2%&0.3%Vol/W) of each andMixture from both oils (0.2% Vol/W) were investigated in refrigerated fresh minced meat. Both oils used had considerableeffectiveness in decreasing Aerobic Plate Count (APC), Coliform Count and Staphylococci Count as well as chemicalindices as pH, Total Volatile Nitrogen (TVN) and Thio Barbituric Acid (TBA) values. Meanwhile, these values decreaseas the concentration of the oil increases with highly significant differences (P<0.05) between these different oils.Marjoram oil (0.3% Vol/W) gives the best effectiveness with a significant advantage in extend shelf life of refrigeratedminced meat to 7 days compared to control group 4 days. Consequently, essential oils can play an important role asantimicrobial agent in refrigerated minced meat and potentially it might be used as a natural preservative ingredient forlonger periods without the need to use hazardous preservatives in food industry, but Marjoram oil is the best one
https://bvmj.journals.ekb.eg/article_31215_2ebb152712bc1b067909162ecbcdf2b6.pdf
2016-09-01
35
42
10.21608/bvmj.2016.31215
marjoram
cumin
Staphylococci
Minced meat
Fahim
Shaltout
1
Department of Food Control, Faculty of Veterinary Medicine, Benha University, Egypt
AUTHOR
Amani
Salem
amani.salem@fvtm.bu.edu.eg
2
Department of Food Control, Faculty of Veterinary Medicine, Benha University, Egypt
AUTHOR
Dalia
Khater
3
Department of Food Hygiene., Animal Health Research Institute., Tanta branch
AUTHOR
Radwa
Lela
4
Department of Food Hygiene., Animal Health Research Institute., Tanta branch
AUTHOR
ORIGINAL_ARTICLE
Studies on bacteriological Profile of some meat products
A total of 100 random samples of meat products represented by luncheon, frozen minced meat, kofta, and sausage (25 ofeach) were collected from different supermarket’s shops in Tanta city These products subjected to bacteriologicalexamination as Aerobic Plate Count (APC), Coliform Count, Staph. aureus count and isolation and identification ofSalmonellae and E-Coli. The result revealed that minced meat showed relatively higher mean values of Aerobic PlateCount as well as Coliform Count (6.07± 0.1) log CFU/g and (3.1±.1) log CFU/g, respectively than the other products.While, it was the lowest one in Staph aureus count (2.2 ± .07) log CFU/g. Regarding to Salmonellae and E-Coli could bedetected in 40% & 32% &12% & 28% & 16% & 24% of minced meat, kofta and sausage, respectively On the other hand,luncheon samples were free from Salmonella and E-coli
https://bvmj.journals.ekb.eg/article_31216_fb01bd20c860cd749d29a3aa88c08aa6.pdf
2016-09-01
43
49
10.21608/bvmj.2016.31216
Minced meat
luncheon
kofta
E-coli
Staphylococcus aureus
aerobic plate count
Fahim
Shaltout
1
Department of Food Control, Faculty of Veterinary Medicine, Benha University, Egypt
AUTHOR
Amani
Salem
amani.salem@fvtm.bu.edu.eg
2
Department of Food Control, Faculty of Veterinary Medicine, Benha University, Egypt
AUTHOR
Dalia
Khaterb
3
Department of Food Hygiene., Animal Health Research Institute., Tanta branch
AUTHOR
Radwa
Lela
4
Department of Food Hygiene., Animal Health Research Institute., Tanta branch
AUTHOR
ORIGINAL_ARTICLE
Traceability of enteropathogenic E. coli in cattle and camel carcasses
A total of 120 random samples of cattle and camel carcasses (60 0f each) were collected from the different abattoirslocated in Qaliubiya governorate. The samples taken from each carcass were represented meat, spleen, liver and kidneys(15 samples of each), the samples were collected directly after slaughtering and evisceration. The collected samples weresubjected to bacteriological examination for detection and identification of E. coli. Enteropathogenic E. coli organismswere 6.67%, 20%, 26.67%, and 40% of the examined samples of cattle meat, spleen, liver and kidney respectively andwere 0.0%, 13.33%, 13.33%, and 33.33% of the examined samples of camel meat, spleen, liver and kidney respectively.The isolated strains were investigated by using Multiplex PCR to detect presence of virulent genes (stx1, stx2 and eaeA)in each isolated Strain of E. coli. O15 Positive strain for stx2 gene, O26 & O111 Positive strains for stx1, stx2 and eaeAgenes. O44 & O126 Positive strains for stx1 gene. O55 Positive strain for stx2 gene and eaeA genes. O91, O103 & O127 Positivestrains for stx1 and stx2 genes O124 negative strains for stx1, stx2 and eaeA genes. The public health importance of theisolated E. coli and the possible sources of contamination of cattle and camel carcasses with E. Coli as well as suggestivehygienic measures to improve the quality of carcasses were discussed
https://bvmj.journals.ekb.eg/article_31217_c64d15e21963ef415aa13388501ea47f.pdf
2016-09-01
50
55
10.21608/bvmj.2016.31217
E. coli
cattle
camel
Carcasses
PCR
Hassan
A.
1
Food hygiene Department, faculty of veterinary medicine, Benha University
AUTHOR
Heikal
I.
2
Animal Health Research Institute, Tanta Branch
AUTHOR
Barhoma
M.
3
Environment Management, Al Qaliubiya Governorate
AUTHOR
ORIGINAL_ARTICLE
Histamine as a quality parameter in marine fish
Histamine is a member of a group of compounds known as biogenic amines; Biogenic amines are biologically activecompounds normally produced by decarboxylation of free amino acids and are present in a variety of foods, eg fish, fishproducts, meat, cheese and fermented foods. The presence of biogenic amines in these foods is an indicator of foodspoilage. Histamine content is an essential quality parameter in sea food quality. In the present study a total of 90 randomsamples of fresh fishes represented by Pagrus, Barboni and Sardine (30 of each) were collected at different times fromvarious fish markets in Gharbia governorate, Egypt, and examined for the presence of histamine by ELISA. The resultsrevealed that 36.7%were acceptable and 63.3% non-acceptable for Sardine, 53.3% were acceptable while 46.7% nonacceptablefor Barboni and 66.7% were acceptable and 33.3% non-acceptable for Pagrus
https://bvmj.journals.ekb.eg/article_31218_ef90d24a0d731dd972b97b361c80f51f.pdf
2016-09-01
56
59
10.21608/bvmj.2016.31218
histamine
ELISA
marine fish
Mohamed
Hassan
mohamed.hassan@fvtm.bu.edu.eg
1
Food Hygiene Department, Faculty of Veterinary Medicine, Banha university
AUTHOR
Nessrine
Aelywah
2
Animal Health Research Institute, Tanta lab
AUTHOR
Fatma
Ali
3
Animal Health Research Institute, Tanta lab
AUTHOR
ORIGINAL_ARTICLE
Molecular characteristics of E. coli contaminating from meat products
A total number of 200 meat products samples (50 each of frozen weight of 25 gram of minced meat, frozen beef burger,frozen kofta and traditional Egyptian sausage) were collected from different markets in Cairo and Giza governorates andexamined by multiplex polymerase chain reaction method for detecting virulence genes of Escherichia coli strainspreviously isolated from frozen minced meat, frozen beef burger, frozen kofta and traditional Egyptian sausage. Thepresent study detected the virulence genes stx1, stx2, eaeA and hlyA genes among 10 Escherichia coli strains. Resultsrevealed that presence of one or more virulence genes in E. coli strains isolated from the examined meat products
https://bvmj.journals.ekb.eg/article_31220_95acc460f14163212a84d3cd20cc53d4.pdf
2016-09-01
60
63
10.21608/bvmj.2016.31220
E. coli
meat product
PCR
Virulence genes
Emara
A.
1
Animal Health Research Institute
AUTHOR
El-shatter
A.
2
Animal Health Research Institute
AUTHOR
Saad
M.
3
Food hygiene Department, faculty of veterinary medicine, Benha University
AUTHOR
ORIGINAL_ARTICLE
Bacteriological and Molecular studies on Methicillin-Resistant Staphylococcus aureus (MRSA) isolated from chicken meat and its products in Kaliobia Governorate
The study was performed on 175 random samples of fresh broiler chicken and chicken meat products viz: Chicken burger;chicken luncheon; chicken kofta and chicken sausage (35 for each), collected from different shops at Benha city, kaliobiagovernment. The sample were examined for detection the prevalence of Staphylococci with special reference to S. aureusand MRSA, beside the phenotypic characterization of the isolated S. aureus strains and detection of their virulence genesin them. The bacteriological examination revealed the isolation of 98 Staphylococcus species including 41 S.aureus; 54S. epidermidis and 3S. chromogenes. In addition, all 41 isolated S. aureus were coagulase positive strains, while, the otherisolated Staphylococcal strains (57) were coagulase negative ones. Moreover, the results of SET- RPLA test revealed that5 S. aureus strains out of 10 randomly examined strains were enterotoxigenic and classified according to type of toxininto (3A; 1 B & 1C). The sensitivity tests for the isolated S. aureus showed strains indicated high resistance to methicillinfollowed by oxacillin; Nalidixic acid; Ampicillin; Amoxicillin; Cefotaxime and Tobramycin, respectively. Meanwhile,they were highly sensitive to Gentamycin; Enrofloxacin; Norfloxacin; Lomefloxacin and Ciprofloxacin. PCR resultscleared that, femA; mecA and hlb virulence genes were detected in all 5 S. aureus studied strains. Meanwhile, ica A wasdetected in 4studied strains; enterotoxin A (sea) was detected in 2 studied strains chicken meat and chicken kofta samplesonly and leukocidin (pvl) virulence gene was detected in 1 studied strain of chicken meat sample
https://bvmj.journals.ekb.eg/article_31221_a51cb3112315cc68df3b549ef72ce353.pdf
2016-09-01
64
72
10.21608/bvmj.2016.31221
Chicken Meat Products
Bacteriological Evaluation
S. aureus
MRSA
PCR
Virulence factors
Ashraf
Abd El Tawab
1
Bacteriology, Immunology and Mycology Dep., Fac. Vet. Med. Benha Univ.
AUTHOR
Fatma
El-Hofyand
2
Bacteriology, Immunology and Mycology Dep., Fac. Vet. Med. Benha Univ.
AUTHOR
Ahmed
Maarouf
3
Animal Health Research "Benhabranch"
AUTHOR
Dina
Mousa
4
veterinarian
AUTHOR
ORIGINAL_ARTICLE
Determination of the optimal protective dose of inactivated Salmonella Typhimurium vaccine in pigeon
Salmonellosis is one of the major health problem affect pigeons and protection of pigeons against this disease is veryimportant, for this purpose the present work was planned to prepare an inactivated Montanide ISA- 206 oil adjuvantvaccine from a locally pigeon isolate of Salmonella Typhimurium (S. Typhimurium) and determined of the optimal dose,which protect pigeon against salmonellosis. Evaluation of such preparation following the quality control tests revealedthat it was free from any foreign contaminants, safe and immunogenic. Pigeons were divided into six groups, three ofthem vaccinated subcutaneously with single dose (0.5ml) of different bacteria concentrations (108,109 and 1010 CFU/dose) and the others three received 2 doses with 3 weeks intervals with same route and concentrations. Thevaccination/challenge assay with a virulent S. typhimurium organism using 5x107 CFU/dose revealed that protection ratesof vaccinated birds were 70% ,75% and 75% for the first three groups (Ia, IIa, IIIa) respectively, while the other threegroups gave 80%, 85% and 85% protection(Ib, IIb, IIIb) respectively.Control groups could not withstand the challengeand protection rate were 15% and 20% respectively. The seroevaluation showed that the humoral immune responsedeveloped against S. typhimurium in vaccinated pigeons was high. On conclusion the optimal dose of the preparedvaccine should be 109 CFU/dose applied twice with three weeks interval, which can cover the needed requirements andprotect pigeon against salmonellosis
https://bvmj.journals.ekb.eg/article_31222_e018123c49d0b9739a96955c8a2a0f8e.pdf
2016-09-01
73
77
10.21608/bvmj.2016.31222
S. typhimurium
Pigeon
vaccine
humoral immune response
Afaf
Khedr
1
Central Laboratory for Evaluation of Veterinary Biologics, Abbasia, Cairo Egypt
AUTHOR
Anhar
Abdel latef
2
Central Laboratory for Evaluation of Veterinary Biologics, Abbasia, Cairo Egypt
AUTHOR
Sayed
L.
3
Central Laboratory for Evaluation of Veterinary Biologics, Abbasia, Cairo Egypt
AUTHOR
Salama
S.
4
Central Laboratory for Evaluation of Veterinary Biologics, Abbasia, Cairo Egypt
AUTHOR
ORIGINAL_ARTICLE
Comparison between effects of Complex enzyme and Multi enzyme supplementation on the Productive Performance of Broiler
This study was conducted to evaluate the effect of two feed additives commercially produced, the complex enzymes andthe multi enzymes on the weekly and finally productive performance of broiler chicks. A total of 600 broiler cobb chicks,housed in an experimental unit, with stocking density 10/ m2, using 10 pens, 60 chicks for each which was used as replicates.Feed and water were provided ad libitum through feeders and bell drinker. Birds were divided into two equal groups.( T1and T2) each of them 300 chicks and five replicates, the first group T1 group chicks fed the basal diet with complexenzymes Allzyme SSF® 0.2 gm/kg feed that composed from Phytase, Protease, Cellulase, Xylanase, Beta-glucanase,Amylase, and Pectinase , the second T2 group was fed on the basal diet with Multi enzymes Natuphos® 0.05 gm/kg feedcomposed of Phytase plus Zympex 008® 0.5 gm/kg feed composed of Alpha-galactosidase, Protease, Beta-mannanase,Cellulase, Xylanase, Beta glucanase, Amylase, and Protease. Productive performance was evaluated by determining theweekly feed intake, body weight, FCR, and final mortality rate and European efficient index. Results revealed significantdifference between the two tested groups within the different weeks the second group showed higher average body weightand lower FCR, compared with the first group finally, there was no significant difference in mortality rate and Europeanefficient index between the two tested groups. It can be concluded that both complex enzymes and multi enzymes havebeneficial effects on the broiler performance parameters
https://bvmj.journals.ekb.eg/article_31225_5092b012c4b55689ce4c9795b4ae7927.pdf
2016-09-01
78
84
10.21608/bvmj.2016.31225
Complex enzymes
Multi enzymes
broiler
Feed additives
Khedr
N.
1
Dep. Nutrition and Clinical Nutrition., Fac. Vet. Med., Benha Univ., Egypt
AUTHOR
Kamelia
Z.
2
Dep. Nutrition and Clinical Nutrition., Fac. Vet. Med., Benha Univ., Egypt
AUTHOR
Tahia
E.
3
Dep. Nutrition and Clinical Nutrition., Fac. Vet. Med., Benha Univ., Egypt
AUTHOR
Elramy
M.
elramyvet@yahoo.com
4
Dep. Nutrition and Clinical Nutrition., Fac. Vet. Med., Benha Univ., Egypt
AUTHOR
ORIGINAL_ARTICLE
Productive performance and behaviour of calf camel (Camelus dromedarius) under semi-intensive and traditional rearing system
A study was carried out on two different rearing systems for observation maternal behaviour during critical period (1st 20days of life), productive performance and feeding behaviour during 3 months of age. Twenty lactating she-camels withtheir calves dromedary (Camelus dromedaries) Sudani camel divided into two groups Group one (n= 10 she-camel withtheir calves) managed in a semi intensive system in private farm in belbes city, all animals were herded during night inclosed pen and set free during the midday. Supplementation consists of concentrates (2kg/day) and roughages (5kg/day)were used. Group two (n= 10 she-camel with their calves) managed in traditional system by pastoralist in met abo alivillage, in sharkia province, Egypt. All the animals were brought to grazing areas where they selected the feed bythemselves from the available plants without any supplemented feeding. The aim of the current study was to evaluatematernal, ingestive behaviour and estimate productive performance of the camel calves under traditional system and semiintensivesystem and its influence on frequency of diarrhea, mortality, morbidity and incidence of mastitis. The resultsindicated that a significant increase in frequency of suckling, duration, calf camel 1st standing frequency drinkingfrequency, duration and grazing duration under semi-intensive system, while calf camel teat seeking frequency, durationand Calf camel1st standing duration with significant decrease during 1st 20 days (critical period), While there was asignificant decrease in grazing frequency in semi-intensive system. A significant increase in diarrhea frequency andincidence of mastitis under traditional system. The body weight and the daily growth rates of the calves under semiintensivesystem were higher than the calves reared in the traditional system. It concluded that, the semi intensiveproduction system showed some advantages for better rearing of calf camels compared with the traditional system. So,supplementing calves by good management under semi-intensive system lead to high productivity and reduction of animallosses
https://bvmj.journals.ekb.eg/article_31226_74ca47dc01b53ab61c244c00f06545b3.pdf
2016-09-01
85
91
10.21608/bvmj.2016.31226
maternal behavior
productive performance
calf camel
Camelus dromedaries
management system
Azhar
Abdel Fattah
1
Vetereinary Public Health Department
AUTHOR
El-Shimaa
Roushdy
shimaa_production@yahoo.com
2
Animal Wealth Development Department, Faculty of Veterinary Medicine, Zagazig University, Zagazig, Egypt
LEAD_AUTHOR
ORIGINAL_ARTICLE
Efficacy of commercial inactivated Salmonella vaccines in quail
This work aimed to evaluate the efficacy of a commercial inactivated Salmonella vaccine in quails where sixty of threeweeks old quails were vaccinated twice via subcutaneous route while another forty birds were kept without vaccinationas non-vaccinated control. The immune response of vaccinated birds was estimated by microagglutination test forSalmonella Enteritidis (SE) and Salmonella Typhimurium (ST) antibodies that revealed a detectable increase in antibodytiters in vaccinated birds in comparison with the unvaccinated ones. The protection rate of vaccinated quails post challengewith locally isolated Salmonella Enteritidis (SE) and Salmonella Typhimurium (ST) strains were 83.3% and 90%respectively. Salmonella was recovered from vaccinated challenged quails at ratio ranged from 23.3% -33.3% for SE and20% - 30% for ST from the heart blood, liver, spleen and caecal junction post challenge. The results of challenge testshowed that vaccinated quails were effectively protected against virulent strains of Salmonella Enteritidis (SE) andSalmonella Typhimurium (ST)
https://bvmj.journals.ekb.eg/article_31227_22643b7c69ca901e6902b4d94c18f646.pdf
2016-09-01
92
95
10.21608/bvmj.2016.31227
Salmonella
vaccine
Quail
Hala
Mahmoud
1
Central Laboratory for Evaluation of Veterinary Biologics, Abbasia, Cairo Egypt
AUTHOR
El-Safty
M.
2
Central Laboratory for Evaluation of Veterinary Biologics, Abbasia, Cairo Egypt
AUTHOR
Hanan
Ahmed
3
Central Laboratory for Evaluation of Veterinary Biologics, Abbasia, Cairo Egypt
AUTHOR
ORIGINAL_ARTICLE
Antioxidant effect of selenium and its Nano form on oxidative stress induced by iron overload
The aim of this study was to demonstrate the antioxidant effect of selenium in its ordinary and Nano form on oxidativestress induced by iron overload in male rat. This study was carried out on 90 rat, weighted 180-200 gm. Rats wereclassified into six groups Group 1: control group. Group 2: Nano selenium-treated group. Group 3: selenium treatedgroup. Group 4: iron overload treated group. Group 5: iron overload + Nano selenium - treatment. Group 6: iron overload+ selenium-treatment. Blood and liver tissue samples were collected from all groups after 1, 3 and 6 weeks from the onsetof the experiment. Serum used for measurement of total iron. Liver tissue was processed for measurement ofmolonadialdhyde (MDA), total antioxidant capacity (TAC), glutathione peroxidase (GSH-PX), super oxide dismutase(SOD), glutathione reduced (GSH) and Catalase. The obtained results revealed that, Nano-selenium + iron and selenium+ iron-treated groups lead to decrease the concentration of MDA, total iron and significant decrease in GSH-PX-1 mRNAexpression and increase the concentration of TAC, catalase, GSH and SOD when compared with iron overload treatedgroup. From the result, it can be concluded that selenium in its ordinary and Nano forms have antioxidant andhepatoprotective effect in experimentally-induced iron overload in male Wister rats. Nano form has superior effects thanordinary form of selenium
https://bvmj.journals.ekb.eg/article_31228_16ad749401b61beec11d837d26326ae6.pdf
2016-09-01
96
102
10.21608/bvmj.2016.31228
iron overload
Oxidative Stress
Nano-Selenium
selenium
antioxidants
Fararh
M.
1
Clinical pathology dept., Faculty of Veterinary Medicine, Benha University
AUTHOR
Farid
S.
2
Clinical pathology dept., Faculty of Veterinary Medicine, Benha University
AUTHOR
Abdalla
A.
3
Clinical pathology dept., Faculty of Veterinary Medicine, Suez Canal University
AUTHOR
Samah
Algharib
4
Clinical pathology dept., Faculty of Veterinary Medicine, Benha University
AUTHOR
ORIGINAL_ARTICLE
Biochemical changes associated with helicobacter pylori infection
The aim of the present study is to through lights on the biochemical relations between H. pylori infection and thechanges in serum Ammonia, Gastrin, Leptin, Amylase, Lipid profile, Iron, Total Iron Binding Capacity, Transferrin,Haptoglobin and Vitamin B12. 90- helicobacter pylori infected patients, and 20- clinically healthy subjects used ascontrol all ages ranged between 25 to over 65 years are applied in the experiment. The result of the present study showedthe association between serum Ammonia and Amylase, Gastrin and Leptin levels, in addition to serum lipid profileincluding (Total cholesterol, Triacylglycerol (TG), Low density lipoprotein – cholesterol (LDL-C) and High densitylipoprotein – cholesterol (HDL-C), Iron status (Iron, Ferritin, Total Iron Binding capacity (TIBC) and Transferrin) inaddition to Vitamin B12 and haptoglobin with helicobacter pylori infection. These parameters may all be regarded aspredictors or risk factors for Gastritis, anemia, peptic ulcer, Gastric cancer and Coronary heart diseases in elderly patients.The findings of the present study suggest that, helicobacter pylori infection are primary interacting mediators in theanemia and different diseases of the stomach
https://bvmj.journals.ekb.eg/article_31229_10ea38215fe2b2498924e317cdb17c06.pdf
2016-09-01
103
109
10.21608/bvmj.2016.31229
H. pylori
Haptoglobin
leptin
ammonia
serum iron
ferritin
Anemia
Abd El- Maksoud
A.
1
Department of Biochemistry, Faculty of Veterinary Medicine, Benha
AUTHOR
Metwaly
M.
2
Department of Biochemistry, Faculty of Veterinary Medicine, Benha
AUTHOR
Fararh
M.
3
Department of Clinical pathology, Faculty of Veterinary Medicine, Benha University, Egypt
AUTHOR
ORIGINAL_ARTICLE
Experimental study on zinc deficiency in sheep
This study was conducted for evaluation of some hematological alteration that associated with Zn deficiency. Eight lambswith average age (5-6) months and body weight (28-30) kg were fed on basal high calcium corn soya bean meal dietwith crude protein, calcium, phosphorus and zinc 14%, 1.6%, 0.8%, 26ppm, respectively. All lambs were fed on theexperimental ration mixture 450-500 gm / Head / day and hay and water ad libitum until the end of the studyat 20th weeks. Serum and whole blood sample were collected weekly for monitoring hematological changescontrol values were determined at zero day before start feeding on the experimental ration. Clinical signsshowed alopecia, skin abnormalities, loss of appetite and emaciation. A significant (p < 0.05) decrease in bodyweight,ruminal movement at 6th week and temperature at 12th week. A significant (p < 0.05) increase in respiratory rate, pulserate appeared from the 8th week till the end of the study. There was significant (p < 0.05) decrease in WBCs, Hb,lymphocyte and RBCs from 4th,6th,4th,10th, weeks, respectively. Serum zinc concentration was significantly decreasing(p < 0.05) from the 6th week. From this study we can conclude that induced Zn deficiency has a negative impact on thegeneral health condition as well as on some hematological and immunological parameters in sheep
https://bvmj.journals.ekb.eg/article_31231_12b0d57b38aa0b5e7b2edbc075b14f4c.pdf
2016-09-01
110
118
10.21608/bvmj.2016.31231
Ibrahim
O.
1
Department of internal medicine Faculty of veterinary medicine – Benha university
AUTHOR
Helal
A.
2
Department of internal medicine Faculty of veterinary medicine – Benha university
AUTHOR
Abd El Raof
M.
3
Department of internal medicine Faculty of veterinary medicine – Benha university
AUTHOR
Elattar
M.
4
Department of internal medicine Faculty of veterinary medicine – Benha university
AUTHOR
ORIGINAL_ARTICLE
Molecular identification of M. bovis BCG by Multiplex PCR
Mycobacterium bovis (M. bovis) BCG belongs to Mycobacterium tuberculosis complex (MTBC), highly relatedorganisms, which are 99.9 % similar at nucleotide level and phenotypically similar. Its differentiation from other membersof MTBC by conventional methods is laborious and time consuming. PCR provides a rapid alternative method fordifferentiation between members of MTBC. It depends on identification of region of difference (RD) which have beenlost during attenuation of M. bovis. Two different BCG strains (from two sources) were confirmed as a member of M.tuberculosis (MTB) complex (MTC) and as BCG strains by PCR using primers to a region of the 16S rRNA gene that isconserved in all mycobacteria and region of difference (RD1, RD4, RD9 and RD12) respectively. DNA of Mycobacteriumtuberculosis was used as a control to compare with BCG strains. The results showed that 16S rRNA gene was present inall tested strains, while the RD1, RD4, RD9 and RD12 were absent only in BCG strains
https://bvmj.journals.ekb.eg/article_31236_c9d3a3a40ddfb1033615efcf830f9a7a.pdf
2016-09-01
119
123
10.21608/bvmj.2016.31236
Region of difference
BCG
PCR
Ashraf
Abd El-Tawab
1
Department of Bacteriology, Immunology and Mycology Fac. Vet. Med. Benha University, Egypt
AUTHOR
Fatma
El-Hofy
2
Department of Bacteriology, Immunology and Mycology Fac. Vet. Med. Benha University, Egypt
AUTHOR
Essam
Nasr
3
Department of diagnostic bacteriology. Veterinary serums and vaccines research Institute, Abbasia, Cairo, Egypt
AUTHOR
Nammalwar
Sriranganathan
4
Department of Biomedical Sciences & Pathobiology, Virginia-Maryland College of Veterinary Medicine, USA
AUTHOR
Enas
Soliman
5
Department of Bacteriology, Immunology and Mycology Fac. Vet. Med. Benha University, Egypt
AUTHOR
ORIGINAL_ARTICLE
Screening the activity of anti-tuberculosis drugs against M. bovis BCG Connaught and M. bovis BCG Pasteur growing in J774A.1 cell line macrophages
Mycobacterium bovis BCG is attenuated strain derived from M. bovis. It has been known to prevent childhood pulmonaryand meningeal tuberculosis and to treat superficial bladder cancer as immunotherapy. Administration of BCG has beenrestricted to immune-deficient individuals since 2007 because of its combined complications. Which treated withantituberculosis drugs based on assumption is fully responsive to these drugs. BCG Connaught showed moresusceptibility to the activity of isoniazid, ethambutol, kanamycin, ofloxacin, streptomycin, amikacin and capreomycinthan BCG Pasteur growing in J774A.1 cells. While the activity of rifampicin and levofloxacin was the same between thetwo tested strains. The spectrum of intracellular drug action can be ordered based on a decreasing order of inhibitoryactivity, as following rifampin > isoniazid > ethambutol > streptomycin > kanamycin> amikacin> ofloxacin>capreomycin> levofloxacin against BCG Connaught. While rifampin > isoniazid > ethambutol > kanamycin =streptomycin > ofloxacin> amikacin> levofloxacin> capreomycin for BCG Pasteur. There is a very limited publicationsdescribe the activity of antituberculosis drugs against intracellular growing BCG strains
https://bvmj.journals.ekb.eg/article_31237_ff804decf59b15968367c623205f7fed.pdf
2016-09-01
124
131
10.21608/bvmj.2016.31237
M. bovis BCG – anti-tuberculosis drugs
macrophages
Ashraf
Abd El-Tawab
1
Department of Bacteriology, Immunology and Mycology Fac. Vet. Med. Benha University, Egypt
AUTHOR
Fatma
El-Hofy
2
Department of Bacteriology, Immunology and Mycology Fac. Vet. Med. Benha University, Egypt
AUTHOR
Essam
Nasr
3
Department of diagnostic bacteriology. Veterinary serums and vaccines research Institute, Abbasia, Cairo, Egypt
AUTHOR
Nammalwar
Sriranganathan
4
Department of Biomedical Sciences & Pathobiology Virginia-Maryland College of Veterinary Medicine, USA
AUTHOR
Enas
Soliman
5
Department of Bacteriology, Immunology and Mycology Fac. Vet. Med. Benha University, Egypt
AUTHOR
ORIGINAL_ARTICLE
Humeral response of cattle to concentrated and purified FMD vaccine using different vaccination programmes
FMD control is largely based on regular vaccination to reduce disease and transmission, in this work we have examinedthe influence of single vaccination and the interval between the first and second vaccinations , the cattle were allotted into4 groups and vaccinated with inactivated concentrated and purified FMD vaccine in different programmes of vaccination,it was found that The double doses vaccination two months interval by polyvalent inactivated concentrated and purified oiladjuvant FMD vaccine enhance the afforded protection duration for vaccinated cattle(prolonged immunity), also this programprovide the vaccinated animals with high protective antibody titer thus high protection against challenge test
https://bvmj.journals.ekb.eg/article_31238_b8a02f16bad9087b73a59dbe3a7621dd.pdf
2016-09-01
132
136
10.21608/bvmj.2016.31238
FMD
cattle
SNT
Vaccination
El-Bagoury
F.
1
Department of virology, Faculty of Veterinary Medicine, Benha University, Egypt
AUTHOR
El-Nahas
M.
2
Department of virology, Faculty of Veterinary Medicine, Benha University, Egypt
AUTHOR
Saad
A.
3
Veterinary Serum and Vaccine Research Institute, Abbassia, Cairo
AUTHOR
Abou-Senna
S.
4
Veterinary Serum and Vaccine Research Institute, Abbassia, Cairo
AUTHOR
ORIGINAL_ARTICLE
Baby Grivet Monkey Kidney (BGM) a new cell line for adaptation of fowl pox virus
The need to economic, sensitive and easily maintained cell cultures use for massive production of fowl pox vaccine wasnecessary. We adapted Fowl pox virus (FPV) Baudette strain on Primary chicken embryo fibroblast (CEF), Baby GrivetMonkey Kidney cell line (BGM) and African green monkey kidney (Vero) cell lines. The highest virus titers were 106.5after the 9th passage in CEF and 106.2 after the 12th passage on BGM while Vero cells were abortive to the virus. BGMcells were selected for study cytopathic effect (CPE) and growth kinetic as it easily maintained than primary CEF cells.The characteristic CPE were rounding till 6th passage with intracytoplasmic inclusion by the 7th passage and syncytiumformation appeared from the 9th passage till the 13th passage. The virus was highly cell associated for the first 84h postinoculation(PI) with a titer of 104.5 then a maximum titer was 106.2 in cell free portion after 120h PI. In conclusion, BGMwere considered a new susceptible cell for growth of FPV with best harvesting time 120h PI to obtain a maximum titerfor subsequent vaccine production
https://bvmj.journals.ekb.eg/article_31239_b1a127a09cd7f719c9cf8d5e88e92cec.pdf
2016-09-01
137
140
10.21608/bvmj.2016.31239
Fowl pox virus
CEF
BGM
VERO cell
El-Bagoury
F.
1
Department of virology, Faculty of Veterinary Medicine, Benha University, Egypt
AUTHOR
El-Nahas
M.
2
Department of virology, Faculty of Veterinary Medicine, Benha University, Egypt
AUTHOR
Olfat
Nakhla
3
Veterinary Serum and Vaccine Research Institute, Abbassia, Cairo
AUTHOR
Nesma
Hassan
4
Veterinary Serum and Vaccine Research Institute, Abbassia, Cairo
AUTHOR
ORIGINAL_ARTICLE
Anatomical study on the spinal arteries in the domestic cat (Felis domesticus)
To study the normal distribution of the arteries that supply the spinal cord of the cat, a colored latex solution was injectedin the aorta of ten adult cats. The specimens were dissected to expose the spinal arteries in this species. The main arterialsupplments of the spinal cord were the ventral spinal and dorsal spinal arteries. These arteries along their courses receivedseveral radicular arteries that arose from the vertebral, dorsal intercostal and lumbar arteries. Comparing the resultsobtained with those commonly described in humans, it is clear that the domestic cat shows a great similarity in mostrespects. However, marked variability of the spinal arteries arising from segmental arteries, the origin of the ventral spinalartery and the origin and presence of the Adamkiewicz artery were recorded. Overall, the data show that, from ananatomical standpoint, the cat seems to be a good model for spinal cord ischemia in humans
https://bvmj.journals.ekb.eg/article_31241_bcac25ed27fa8eb06ac61655cd17a0b8.pdf
2016-09-01
141
146
10.21608/bvmj.2016.31241
Anatomy-Spinal arteries
domestic cat
Farag
M.
1
Department of Anatomy, Faculty of Veterinary Medicine, Cairo University, Egypt
AUTHOR
Wally
R.
2
Department of Anatomy, Faculty of Veterinary Medicine, Cairo University, Egypt
AUTHOR
Khalifa
F.
3
Department of Anatomy, Faculty of Veterinary Medicine, Cairo University, Egypt
AUTHOR
Nafady
A.
4
Department of Anatomy, Faculty of Veterinary Medicine, Cairo University, Egypt
AUTHOR
ORIGINAL_ARTICLE
Effect of level of biosecurity measures on prevalence of some pathogenic microorganisms in poultry farm
The present study was performed to evaluate the efficiency of disinfection process applied in poultry farms anddetermination the major input of infection into poultry farms. A total of 140 samples and swabs were collected from farmA and B with an average of 70 samples from each farm. These samples were collected from floor, walls, drinker, feeder,air, windows, fans, boats, car rubber, cloaca, litter, water, feed and hands samples (5 of each) from two broiler farmsbefore and after disinfection. The results showed that Salmonella Kentuckey, Salmonella Typhymurium, SalmonellaEnteritidis and Salmonella Virchow were higher in different samples taken from farm (A) than in different samples takenfrom farm (B). Actually, E. coli serotype O1 was detected in drinker, boots, cloaca, litter (20% of each) & car rubber(40%) of farm (A) and only from car rubber (20%) of farm (B). Moreover, E. coli serotype O2 was isolated from fan,feeder, feed (20% of each) & boots (40%) of farm (B). However, E. coli serotype O78 was detected in floor, feeder,cloaca, water (20% of each) & feeder & litter (40% of each) of farm (A) and feeder, cloaca (20% of each) & litter (40%)of farm (B). In concern to S. aureus, the isolation percentages from wall, fan, cloaca, litter (20% of each), floor, drinker,boots, air (40% of each), car rubber (60%) & worker hands (80%) of farm (A) and only from drinker & car rubber (20%of each) of farm (B). While, C. jejuni was recovered floor, wall, fan, feeder, boots, water (20% of each), drinker, cloaca(40% of each) & litter (60%) of farm (A) and only from cloaca and litter (20% of each) of farm (B).
https://bvmj.journals.ekb.eg/article_31242_e0d9c16623ed7396b094ba06ba39c467.pdf
2016-09-01
147
153
10.21608/bvmj.2016.31242
disinfectants
S. typhimurium
E. coli
S. aureus
Broiler farm
Abdelal
A.
1
Department of Animal Hygiene Behavior and Management, Faculty of Veterinary Medicine, Benha University
AUTHOR
Tulip
Abdulghaffar
2
Department of Animal Hygiene Behavior and Management, Faculty of Veterinary Medicine, Benha University
AUTHOR
Halla
El Bahgy
3
Department of Animal Hygiene Behavior and Management, Faculty of Veterinary Medicine, Benha University
AUTHOR
Metawea
F.
4
Department of Animal Hygiene Behavior and Management, Faculty of Veterinary Medicine, Benha University
AUTHOR
Kamel
H.
5
Officer of the armed forces
AUTHOR
ORIGINAL_ARTICLE
Effect of some disinfectants on some pathogenic microorganisms isolated from poultry farm
This study was conducted as a field study to select the most effective disinfectants for control of the microbialcontamination in poultry farms through evaluation of some commercial disinfectants. All tested commercial disinfectantswere diluted with sterile tap water and applied at different concentrations (0.5 and 1.0 %) on contaminated surfaces(concrete) by spraying at a rate of 0.5 liter/m2. The Aerobic Plate Count (APC) on naturally contaminated floor of broilerfarm was determined before the application of commercial disinfectants at different concentrations with contact times of15, 30, 60, and 120 minutes. The results revealed that disinfectants at concentration of 0.5% had little action on the testedpathogens as S. Typhimurium, E. coli and S. aureus after 30 and 60, 90 and 120 minutes contact time. While, applicationof Virudox-G, Glutarol and Alkadox at concentration of 1% for 120 minutes contact time was efficient and proved 100%reduction either for of the pathogens count. While, Phenodex showed the lowest inhibitory effect on these pathogens
https://bvmj.journals.ekb.eg/article_31243_0cee4d451afe0e5ab1efe40547d8785e.pdf
2016-09-01
154
158
10.21608/bvmj.2016.31243
disinfectants
S. typhimurium
E. coli
S. aureus
Broiler farm
Abdelal
A.
1
Department of Animal Hygiene Behavior and Management, Faculty of Veterinary Medicine, Benha University
AUTHOR
Tulip
Abdulghaffar
2
Department of Animal Hygiene Behavior and Management, Faculty of Veterinary Medicine, Benha University
AUTHOR
Halla
El Bahgy
3
Department of Animal Hygiene Behavior and Management, Faculty of Veterinary Medicine, Benha University
AUTHOR
Metawea
F.
4
Department of Animal Hygiene Behavior and Management, Faculty of Veterinary Medicine, Benha University
AUTHOR
Kamel
H.
5
Officer of the armed forces
AUTHOR
ORIGINAL_ARTICLE
Pathological and biochemical studies on ochratoxicosis in balady duckling with trail of treatment
A total of 100 healthy balady ducklings, one day old were divided into 4 equal groups (25 in each), Gp (1) kept as controlgroup, Gp (2 and 3) received 0.5 mg ochratoxin/kg ration and 0.5ml antiochratoxin/liter drinking water for 30 consecutivedays respectively and Gp (4) received both ochratoxin and antiochratoxin in same dose, period and route of administration.Ducklings supplemented with ochratoxin displayed significant reduction in weight gain, feed conversion rate, RBCs,WBCs, Hb, PCV, T protein albumin, globulin, T. lipid, cholesterol, triglycerides CAT and SOD, coupled with significantelevatoin in feed conversion rate, mortality rate, AST, ALT, ALP, uric acid, creatinine and MDA Ducklings received antiochratoxinshowed significant increase in weight gain, RBCs, Hb, total protein, albumin Catalase and super oxide dismutasebeside in signif- icant increase in PCV,WBCs, globulin, AST, ALT, ALP, uric acid and creatinine, triglycerides cholesteroland total lipid coupled with insignificant decrease in MDA and improved in feed conversion rate. Hemato-biochemicalprofiles and antioxidant revealed significant improvement in antiochratoxin treated duckings when compared withochratoxinated duckings. Ochratoxin residues in liver and kidney were high at 1st day of clearance period and disappearedat 10th days of clearance period. Alteration in histological picture in internal organ in duckling received ochratoxin revealedmassive degenerative changes in renal epithelial cells of proximal and distal convoluted tubules (Nephrotoxicity) andmassive necrosis of some tubular epithelial cells. Beside severe hepatic damage and also lesions in the immune organs andintestine. In general, histological changes were mild in birds receiving antiochratoxin alone or with ochratoxin. It could beconcluded that, ochratoxicosis in duckling resulted in poor feed intake, weight gain and feed conversion rate, had adverseeffect in body weight, hemato-biochemical parameters beside decreased blood antioxidant; anti-ochratoxin improved theseparameters in duck
https://bvmj.journals.ekb.eg/article_31244_51fc1773421d57a978a203ebe1e718db.pdf
2016-09-01
159
166
10.21608/bvmj.2016.31244
duckling
Ochratoxin
body weight
mortality rate
blood picture
biochemical parameters
Antioxidant enzymes
Pathology
Abo El -Fetouh
H.
1
Histopathology, departments, Animal Health Research Institute (Zagazig branches)
AUTHOR
Heba
A.
2
Histopathology, departments, Animal Health Research Institute (Zagazig branches)
AUTHOR
Halla
S.
3
biochemistry, departments, Animal Health Research Institute (Zagazig branches)
AUTHOR
Ghada
El Kader
4
biochemistry, departments, Animal Health Research Institute (Zagazig branches)
AUTHOR
Nahed
kamora
5
clinical pathology, departments, Animal Health Research Institute (Zagazig branches)
AUTHOR
ORIGINAL_ARTICLE
Prevalence of blaTEM and blaSHV genes in genomic and plasmid DNA of ESBL producing Escherichia coli clinical isolates from chicken
A total of 102 chicken samples were collected from different farms in Qalubia, Behera, Cairo, Assuit and MenofiaGovernorates. The samples were represented by liver, spleen, lungs, heart blood, intestine and kidneys and subjected forisolation and identification of Escherichia coli. The bacteriological examination of the samples indicated the isolation of55 E. coli represented as 31, 6, 8, 5, and 5 from Qalubia, Behera, Cairo, Assuit and Menofia, respectively. Differentserotypes of E. coli (O158, O125, O111, O27, O20, O6, O25, O26, O145, and O159) and12 untyped strains were demonstrated.Thirty-Two E. coli isolates were subjected to initial screening test for extended-spectrum beta-lactamases (ESBLs)production by disc diffusion method with various cephalosporins. The results showed that 46.9% of samples weresensitive to ceftriaxone and cefotaxime and 53.1% to ceftazidime. By double disc synergy test, 19 E. coli strains wereidentified as ESBL producers. PCR results of 32 E. coli isolates showed that blaTEM gene was detected in the genomicDNA of all isolates and in plasmid DNA of 18 isolates. While, blaSHV was detected in the genomic DNA of 12 isolatesand in plasmid DNA of 9 isolates. We can conclude from the current results that amplification of both genomic andplasmid DNA increase the positivity of detection in comparison with amplification of each of DNAs alone
https://bvmj.journals.ekb.eg/article_31245_3b89dfb30208b2dc11e139ff5ea24465.pdf
2016-09-01
167
177
10.21608/bvmj.2016.31245
E. coli
ESBL
Plamid
blaTEM and blaSHV
Ashraf
Abd El Tawab
1
Bacteriology, Immunology and Mycology Dept., Fac. Vet. Med. Benha Univ.
AUTHOR
Fatma
El-Hofy
2
Bacteriology, Immunology and Mycology Dept., Fac. Vet. Med. Benha Univ.
AUTHOR
Manar
El-khayat
3
Bacteriology, Immunology and Mycology Dept., Fac. Vet. Med. Benha Univ.
AUTHOR
Heba
Mahmoud
4
Reference Laboratory for Veterinay Quality control on poultry production, Dokki-Giza
AUTHOR