ORIGINAL_ARTICLE
Staphylococci in some meat products
Meat products are liable to harbor different types of micro-organisms during long chain of handling, processing, distribution and storage as well as preparation, so a total of 90 random samples of locally manufactured meat products represented by frozen beef burger (30), kofta (30) and sausage (30) "25 grams of each" were randomly collected from different shops in El-Menoufia governorates, Egypt. It is evident from the obtained results that all the examined samples of beef burger, kofta and sausage were positive Staphylococci On the other hand,S.aureus detected in 40%, 46.67% and 63.33% in examined samples of beef burger, kofta and sausage, respectively. Serological examination declared that the incidence of Staphylococcus species from examined beef burgers were 40%, 23.33% and 3.33% for S.aureus, S.epidermidis and S.intermedius, respectively. While, in kofta were 46.67%, 26.67% and 6.67% for S.aureus, S.epidermidis and S.saprophyticus, respectively and in sausage were 63.33%, 36.67%, 10%, 13.33 and 6.67 for S.aureus, S.epidermidis, S.intermedius, S.saprophyticus and S. xylosus, respectively. Whilst, the detected S.aureus enterotoxins from examined samples were enterotoxin B in beef burger with incidence of 3.33%, enterotoxin A and enterotoxin C with incidence of 3.33% and 3.33, respectively from kofta samples. While in sausage sample enterotoxin A, B and D&C were detected with incidence of 10%, 3.33% and 3.33%, respectively.The presence of these microorganisms in large numbers make these meat products of inferior quality and unfit for human consumption.
https://bvmj.journals.ekb.eg/article_53507_6fdf982c09951550e0f1cad23aaf862a.pdf
2018-03-01
1
9
10.21608/bvmj.2018.53507
Staphylococci
S. aureus
Beef burger
kofta
sausage
Enterotoxins
Fatens
Hassanina
1
Food Control Dep., Fac. Vet. Med., Benha Univ.
AUTHOR
Mohamed
Hassana
2
Food Control Dep., Fac. Vet. Med., Benha Univ.
AUTHOR
Shimaa
Nada
3
Animal Health research institute, Shebin Elkoom branch.
AUTHOR
Sarah
Badr
4
Animal Health research institute, Shebin Elkoom branch.
AUTHOR
ORIGINAL_ARTICLE
Assessment of microbiological critical control points in Food processing and serving plant
Restaurant chains are considered attractable places to people around the world; however prepared meals may be contaminated intentionally or accidentally. The examined samples (200) were collected from stages of preparation of grilled chicken (60), casserole beef and hawawshi (50 of each) as well as swabs from cutting boards, knives, mincer and worker hands (10 of each) in a food serving plant. The collected samples were bacteriologically examined for monitoring of APC, Enterobacteriaceae count, Coliform count, Salmonellae, S.aureus, E.coli and Cl. perfringens. The obtained results revealed that the most contaminated product was casserole beef followed by hawawshi and grilled chicken. The incidence of food borne pathogens were S.aureus (32%), Salmonellae (30.2%), E.coli (26.6%) and the lowest one was Cl.perfringens (8.8%).
https://bvmj.journals.ekb.eg/article_53510_85607d7f6df379b8e59a3b16f73e035a.pdf
2018-03-01
10
20
10.21608/bvmj.2018.53510
Bacteriological – plant –chicken –beef
hawawshi
Amani
Salem
amanisalem@yahoo.com
1
Food Control Dep., Fac. Vet. Med., Benha Univ.,
AUTHOR
Zakaria
I.M.
2
Animal Health Research Institute, Dokki, Giza
AUTHOR
Anwar
M.O.
3
Animal Health Research Institute, Dokki, Giza
AUTHOR
ORIGINAL_ARTICLE
Relationship between causes of death and early post mortem hepatic DNA degradation
The pattern of postmortem DNA degradation is frequently used as indicator of postmortem interval, but it could be affected by many internal and/or external factors. The main objective of the present study is the analysis of postmortem hepatic DNA damage in relation to time passed since death under the impact of different causes of death in order to improve our understanding about the effect of cause of death on the characters of postmortem hepatic DNA fragmentation. For this purpose, our study was carried out on four groups of rats (n=6).Animals were experimentally killed by slaughtering, electrocution, drowning and zinc phosphide poisoning, respectively. Liver samples were collected at zero, 2 h, 6 h, 12 h and 24 h postmortem time points. Analysis of postmortem DNA denaturation was undertaken using gel electrophoresis. Obtained data revealed presence of marked effect of the cause of death on the pattern of DNA smearing after death. Hepatic DNA of slaughtered rats showed the highest resistant to degradation till 24 h after death, while the poisoning by zinc phosphide enhance the rate of DNA degradation over all other groups. DNA band smearing in drowned rats appeared relatively earlier than those electrocuted, but the both occupied average site between slaughtered and zinc phosphide-intoxicated animals. It can be concluded that cause of death must be taken into account as a major factor could modify the early postmortem hepatic DNA damage.
https://bvmj.journals.ekb.eg/article_53516_b97ceb50f614cc21aa7e9ab32d13d217.pdf
2018-03-01
21
27
10.21608/bvmj.2018.53516
cause of death
DNA degradation
Electrophoresis
Postmortem interval
Osama
El Okle
1
Department of Forensic Medicine and Toxicology, Faculty of Veterinary medicine, Alexandria University, Egypt
AUTHOR
Omnia
El Euony
2
Department of Forensic Medicine and Toxicology, Faculty of Veterinary medicine, Alexandria University, Egypt
AUTHOR
Nasr
Sherif
3
Department of Genetics, Faculty of Veterinary medicine, Damanhur University, Egypt
AUTHOR
ORIGINAL_ARTICLE
Bacteriological status of chicken meat products marketed at Menofia governorate
A total of 90 random samples of semi-cooked chicken Pane, Nuggets and Strips products (30 samples of each) were collected from different supermarkets in different districts at Monofia governorate for determination of their bacteriological aspects. The obtained results indicated that the mean values of total bacterial count, total Enterobacteriace and total coliforms counts/g in the examined samples were 4.25x 106 1.40 x 106, 5.47 x 104 1.98 x 104 and 8.32x 103 3.33x 103 for pane, 7.12 x 106 2.11 x 106, 6.58 x 104 1.98 x 104 and 6.87 x 103 2.00 x 103 for Nuggets and 5.96 x 106 1.49 x 106, 6.19x 104 1.30x 104 and 5.49x 103 2.00 x 103 for Strips, respectively. Furthermore, Staphylococcus aureus, E.coli and Salmonella could be detected in examined sample with different percentages. The public health significances of isolated bacteria were discussed.
https://bvmj.journals.ekb.eg/article_53518_c19bf054f2cbbd5f975a113249b47ea2.pdf
2018-03-01
28
40
10.21608/bvmj.2018.53518
Chicken Pane
Nuggets
Strips
Salmonella
E.coli
Staph aureus
Fahim
Shaltout
fahimshaltout@yahoo.com
1
Med., Benha Univ., ., Fac. Vet.tFood Hygiene Dep
AUTHOR
Dina
El Zahaby
2
Kom-Shebin El ., Animal Health Res.,tFood Hygiene Dep
AUTHOR
Lamiaa
Lotfy
lamiaalotfy@yahoo.com
3
niversityUsheikh -, Kafer elducationEpecific Sof acultyFconomics, Eome Hf
AUTHOR
Hala
El Shorah
4
Med., Benha Univ., ., Fac. Vet.tFood Hygiene Dep
AUTHOR
ORIGINAL_ARTICLE
Quality assurance of some locally processed meat products
One hundred random samples of meat products represented by 25 samples each of minced meat, beef burger, sausage and luncheon which were collected from different supermarkets in Cairo and Giza cities, Egypt. Each sample was packed in plastic bag and transferred immediately to the laboratory in an icebox with a minimum period of delay to be examined organoleptically, bacteriologically and chemically. Organoleptic evaluation, regarding color, odor and taste, the percentages of undesirable samples were 28%, 28% and 24%, 20%, 20% and 16%, 24%, 20% and 24% and16%, 16% and 24% of minced meat, beef burger, luncheon and sausage, respectively. Bacteriologicalevaluation, regarding minced beef, the mean values of APC, Enterobacteriaceae and Staphylococcus aureus count was 3.3x105, 4.27x103 and 0.2x102,respectively and the number of positive samples to E.coli and salmonella was 4 and 3. Regarding beef burger, the mean values of APC, Enterobacteriaceae and Staphylococcus aureus count was 1.6x104, 7.12x102 and 0.1x102 , respectively and the number of positive samples to E.coli and salmonella was 3 and one. Regarding luncheon, the mean values of APC, Enterobacteriaceae and Staphylococcus aureus count was 2.3x103, 5x102 and <102 , respectively and the number of positive samples to E.coli and salmonella was 3 and 2. Regarding sausage, the mean values of APC ,Enterobacteriaceae and Staphylococcus aureus count was 4.5x105, 7x103 and0.3x102,respectively and the number of positive samples to E.coli and salmonella was 5 and 6. Chemical examination, regarding minced beef, the results revealed that the mean values of pH, TVN and TBA were 5.89, 24.69 and 0.70, respectively and the percentages of accepted samples regarding TVN and TBA were 60 % and 76%, respectively. Regarding beef burger, the mean values of pH, TVN and TBA were 5.8, 17.01 and 0.44, respectively. Regarding sausage, The mean values of pH, TVN and TBA were 5.9, 16.23 and 0.45, respectively and the percentages of accepted samples of sausage regarding TVN and TBA were 92% and 100%, respectively. Regarding luncheon, the mean values of pH, TVN and TBA were 5.9, 22.01 and 0.25 respectively.
https://bvmj.journals.ekb.eg/article_53519_d51f0b8e4a590abb331a2aaefcf1b8bc.pdf
2018-03-01
41
47
10.21608/bvmj.2018.53519
Quality
processed meat
microbiological
organoleptic
TVN
tba
Fatin
Hassanien
fatinhassanien@yahoo.com
1
Department of food control (meat hygiene). Faculty of Veterinary Medicine, Benha University.
AUTHOR
Shaltout
Fahim
2
Department of food control (meat hygiene). Faculty of Veterinary Medicine, Benha University.
AUTHOR
Hashim
Mohammed
3
Animal Health Research Institute, Dokki branch, Giza
AUTHOR
Lamiaa
Lotfy
lamiaalotfy@yahoo.com
4
Department of Home economics, Faculty of specific education, Kafr El-Sheikh university.
AUTHOR
Hatem
El- Nagar
5
Department of food control (meat hygiene). Faculty of Veterinary Medicine, Benha University.
AUTHOR
ORIGINAL_ARTICLE
Omega-3 polyunsaturated fatty acids as therapeutic options for the treatment of ethanol induced gastric ulcer in rats
Gastric ulcer is a common chronic disease in human digestive system. Massive alcohol drinking can lead to gastric ulcer. In the present study we investigated the gastroprotective effect and the molecular mechanisms of Omega 3 polyunsaturated fatty acids(ώ-3 PUFAs) in a rat model of ethanol-induced gastric ulcer.42 male rats were divided into six equal groups. Group 1 :( Control normal group) rats received no drugs. Group 2 :( Omega-3 group): rats receivedOmega-3 orally(244.8 mg/kg b.wt/day) for 14 days. Group 3 :( early ulcer group):rats received absolute Ethanol (0.5ml/rat) orallyand sacrificed one hour later after ethanol administration Group 4: (Early ulcer + Omega-3 protected group) rats received omega-3 similar to group 2 for 14 days prior to absolute ethanol administration, then sacrificed after one hour. Group5: (late ulcer group) rats received absolute ethanol similar to group 3 and sacrificed after 14 days. Group6:( Late ulcer + Omega-3 treated group)rats first administered with absolute ethanol then after one hour omega-3 was administered for 14 days. The results showed significant increase in No,L-MDA,GSH with marked decrease in CAT activity in stomachof ethanol- induced gastric ulcer in rats as compared with control normal group.However, a significant depletion of gastric tissue No, L-MDA and GSH concentrations and marked increase in CAT activity were observed after omega-3 treatment compared to ulcerated untreated rats. The qPCR results revealed a significant up-regulation of mRNA gene expression levels of TNF-a, MOP and iNOS in stomach of ethanol-induced gastric ulcer in rats. This expression was significantly down-regulatedafter administration of omega 3.Conclusively, omega-3 protects rat gastric mucosa against ethanol-induced gastric ulcer via anti-inflammatory and anti-oxidative mechanisms.
https://bvmj.journals.ekb.eg/article_53523_41899f58795e69a6558750bd562d442f.pdf
2018-03-01
48
65
10.21608/bvmj.2018.53523
omega-3
ethanol, gastric ulcer
Oxidative Stress
Anti-inflammatory
Yakout
El Senosi
1
Biochemistry Department, Faculty of Vet. Med., Benha University, Egypt.
AUTHOR
Mohamed
Mahfouz
2
Biochemistry Department, Faculty of Vet. Med., Benha University, Egypt.
AUTHOR
Alaa
Hussein
3
Biochemistry Department, Faculty of Vet. Med., Benha University, Egypt.
AUTHOR
ORIGINAL_ARTICLE
Bacteriological and Molecular Studies on some Bacteria Isolated From Mastitic Cattle and Humans Contact
This study was done on a total of 92 mastitic milk samples (50 clinical, 42 sub-clinical) and 40 hand swabs from contact humans were collected from different dairy farms at Gharbia governorate. The collected samples were examined bacteriologically to isolate mastitis pathogens (Staph. aureus, Strept. agalactiae, S. dysgalactiae and Strept. uberis). From clinical mastitic samples, six isolates were S. aureus ( 12%) and one isolate was (2%) S. dysgalactiae. Among sub clinical mastitic milk samples two isolates were S. aureus (4.6%) and one isolate (2.3%) S. agalactiae. While S. uberis were not detected. From contact human hand swabs both S. aureus and Streptococcus species were s were highly sensitive to Antibiotic sensitivity test revealed that all bacterial isolatenot detected. gentamicin respectively, while all isolates andenerofloxacin, ciprofloxacin, sulpha |trimethoprim were resistant to penicillin followed by amoxicillin/Clavulanic acid. Two isolates of S. aureus were screened for detection of enterotoxin genes (Sea, Seb, Sec, Sed and See) by multiplex PCR. Only Sed gene was detected in one isolate. Cfb gene (CAMP factor) and hyl (hyluronidase) gene were detected in S. agalactiae. mig (surface-expressed mig protein) gene was detected in S. dysgalactiae
https://bvmj.journals.ekb.eg/article_53524_54e82f15bb84a182c23b5975b46d8fa8.pdf
2018-03-01
66
87
10.21608/bvmj.2018.53524
Mastitic bacteria
cattle
Humans contact
Ashraf
A. Abd EL Tawab
1
Bacteriology, Immunology and Mycology Department Faculty of Veterinary Medicine, Benha University
AUTHOR
Fatma
El Hofy
2
Bacteriology, Immunology and Mycology Department Faculty of Veterinary Medicine, Benha University
AUTHOR
ElKhalid
ekhnawey
3
Animal Health Research Institute.Dokki, Egypt
AUTHOR
Fawzia
El-Shenawey
4
Animal Health Research Institute, tanta, Egypt
AUTHOR
ORIGINAL_ARTICLE
Phenotypic and Genotypic characterization of Vibrio species isolated from marine fishes
Vibriosis is considered the most important threatening disease problem facing aquaculture. The bacteria occur widely in aquatic environment and are part of the normalflora of coastal seawater and are opportunistic pathogens in marine animals.A total number of (311 ) fish ,(97 )Seabream , ( 118 ) Seabass, (96 ) Mugil capito were collected from Kafr Hamedo and Ezzbet El Borg marine water fish farm (Damietta governorate), El-Manzala and Mansoura Fish Market (EL-Dakahlia governorate)in the period from March 2016 to April 2017 during the four seasons. The clinical examination of diseased fish revealed that, presenceof redness at base of anal fin and erosion of caudal fin, presence of ulcersin skin, darkness of the skin, detached scales, fin erosion, corneal opacity and palegills. Post mortem examination of infected fishes revealed that, liver appearedenlarged, congested or pale with engorged gall bladder, splenomegaly, congestedkidney and hemorrhage in abdominal cavity. Congested gills with excessiveamount of mucous and enlarged liver with hemorrhagic patches on itsedges.The isolated bacteria on thiosulfate citratebile salt sucrose agar (TCBS) gave yellow colonies for Vibrio alginolyticus and gave green colonies for Vibrio parahemolyticus. The strains were tested according to their susceptibility as resistant, intermediate or sensitive for each antibiotic group. Vibrio alginolyticus and Vibrio parahemolyticus revealed sensitivity to ciprofloxacin (Cipro5µg) and novobiocine (NV30µg). Moreover intermediate sensitivity was found to oxytetracycline (OT 30 µg). While resistance was observed to amoxicillin (Aml 10 µg) and cholistine sulphate (CT 10 µg).The isolated bacteria (Vibrio alginolyticus and Vibrio parahemolyticus) were 171/311 (54.98%) from infected fish where the total number of Seabass 72/118 (61%), Seabream 47/97 (48.45%) and Mugil capito 52/96 (54.17%). The highest infection rate was recorded by summer (100% , 82.61% , 80%), winter (73% , 58.33% , 54.54%) , spring (56% , 42.11% , 69.23%) and autumn (36% , 30.23% , 40.38%) for Seabass, Seabream and Mugil capito respectively.PCR yielded a single specific and clear amplificated band ofexpected size 737bp for collagenase gene and 387bp for pR72H gene for V. alginolyticus and V. parahemolyticus respectively.
https://bvmj.journals.ekb.eg/article_53527_c087a6ab57e46a17a98de8ecac760f74.pdf
2018-03-01
79
93
10.21608/bvmj.2018.53527
V. alginolyticus
V. parahemolyticus
Marine Fishes
specific genes (collagenase and pR72H)
Ashraf
Abd El Tawab
ashrafabdeltawab@yahoo.com
1
Bacteriology, Immunology and Mycology Department, Faculty of Veterinary Medicine, Benha University
AUTHOR
A.M
Ibrahim
2
Department of Microbiology, Animal Health Research Institute, Mansoura, Egypt
AUTHOR
Ahmed
Sittien
3
Researcher of Microbiology, Faculty of Veterinary Medicine, Benha University
AUTHOR
ORIGINAL_ARTICLE
Phenotypic and genotypic characterization of listeria species isolated from chicken and milk products
Listeria monocytogenes has became increasingly important as a food-associated pathogen. It can cause a rare but serious disease called listeriosis with high fatality rates (20–30%) compared with other foodborne microbial pathogens. To estimate the incidence and levels of Listeria spp. in different food sources, A total of 200 random samples which collected from different sources 65 samples isolated from poultry , 45 from poultry byproducts (Frozen chicken meat balls (kofta), Frozen chicken burger and frozen chicken sausages), 20 from pasteurized milk ,30 from milk products (Feta cheese ,cream cheese )and 40 from poultry eggs .Only 7(14%) of samples that collected from suspected isolates and detected on Oxford agar, PALCAM agar and ALOA agar were suspected to be Listeria spp. In poultry, poultry byproducts, pasteurized milk, milk byproducts and poultry eggs, listeria percentage were 0/65(0%), 4/45(1.8), 0/20(0%), 0/30(0%) ,3/40(1.2%) isolates, respectively. The in-vitro antimicrobial sensitivity test showed that the isolated L.monocytogenes were sensitive to Sulphamethoxazole /trimethoprim, Gentamycin ,Ceftazidime and Cephazoline followed by Sulphamethoxazole, ceftriaxone , weak sensitivity to Penicillin . While they were resistant to Erythromycin, Clindamycin, Ampicillin. The results of virulence tests for isolated listeria strains appeared that all of L.monocytogenes were virulent strains as all of them were positive to CAMP test; showed narrow zone of β–hemolysis on sheep blood agar .The PCR results for Listeria isolates showed that all strains are L.monoctogenes and have Inl A and hly A genes positive as virulence genes while negative Inl B, iap and prf A genes.
https://bvmj.journals.ekb.eg/article_53531_3385f300986f3a61b95c908a9e2de518.pdf
2018-03-01
94
105
10.21608/bvmj.2018.53531
L.monocytogenes
Poultry
Milk
Ashraf
Abd El Tawab
ashrafabdeltawab@yahoo.com
1
Bacteriology,Immunology and Mycology Dep., Fac.Vet.Med.Benha Univ
AUTHOR
Wafaa
Hassan
2
Animal Health Research
AUTHOR
Fatma
El- Hofy
3
Bacteriology,Immunology and Mycology Dep., Fac.Vet.Med.Benha Univ.
AUTHOR
Haidy
Zaki
4
Animal Health Research
AUTHOR
ORIGINAL_ARTICLE
Trial for Preparation of Combined Equine Influenza virus, Equine Herpesvirus-1 and Tetanus Toxoid Vaccine
Vaccination remains an important strategy to fight Equine influenza (EI), Equine herpesvirus-1 (EHV- 1) and tetanus infection, in combination with management measures. A combined inactivated EIV, EHV-1 and Tetanus toxoid (TT) vaccine adjuvanted with saponin and Alhydrogel was prepared and tested in four groups of guinea pigs in comparison with each vaccine separately beside another group as control. The humoral immune response in vaccinated guinea pigs against EI, EHV-1 and tetanus was evaluated by Haemagglutination Inhibition (HI) antibody titers, Enzyme linked immunosorbent assay (ELISA) titers, and toxin neutralizing (TN) antibody titers respectively. All vaccines proved to be safe and potent in guinea pigs. Also, the obtained antibodies titers with combined vaccine was nearly similar to titer obtained with separated vaccine of each target agent in vaccinated guinea pigs. The evaluation of keeping quality of the prepared combined inactivated vaccine showed that it is was stable at 4C up to 12 months and for 3 months at room temperature.
https://bvmj.journals.ekb.eg/article_53535_28521c62a7d2991431bfbd53ac156f8f.pdf
2018-03-01
106
116
10.21608/bvmj.2018.53535
Equine influenza
herpesvirus-1
tetanus toxoid
saponin
Alhydrogel vaccine
Potency
Mohamed
bayoumi
1
Equine Viral Disease Research Department, Veterinary Serum and Vaccine Research Institute, Abbasia
AUTHOR
Abdelmoneim
Moustafa
profmoneim@yahoo.com
2
Animal medicine department, Faculty of Veterinary Medicine, Benha University
AUTHOR
Nashwa
Madkour
3
Equine Viral Disease Research Department, Veterinary Serum and Vaccine Research Institute, Abbasia
AUTHOR
Abdelfattah
Selim
abdelfattah.selim@fvtm.bu.edu.eg
4
Animal medicine department, Faculty of Veterinary Medicine, Benha University
AUTHOR
Wafaa
Abdelazez
5
Equine Viral Disease Research Department, Veterinary Serum and Vaccine Research Institute, Abbasia
AUTHOR
ORIGINAL_ARTICLE
Biochemical effect of Lysine – cetrimonium zinc compound on 7,12dimethylbenz(a)anthracene Induced Mammary Carcinogenesis in Rats
Breast cancer is the second leading cause of cancer death in women in the world .The chemopreventive effect of Lysine-Cetrimonium Zinc Complex with zinc oxide nanoparticle and sodium ascorbate (L CZN) on 7,12-Dimethylbenz[a]anthracene(DMBA) induced mammary carcinogenesis was investigated in(60) female rats aged from (4-5)weeks and weighting from (90- 110) gr . Oral dose of DMBA (100mg/k.gr.b.wt). induced a significant increase in serum ALT, AST, MDA, urea, Creatinine, and CD20 in serum or tissue .However were observed in DMBA induced mammary cancer in rats depletion in CAT,GSH and Annexin V were markedly decreased . Administration of (NNC) Novel Nano composite was able to mitigate mammary carcinogenic damage induced by DMBA as to directory pronounce chemo preventive effect against lipid peroxidation, sever enzymatic changes and maintained glutathione status and antioxidant enzymes in addition histopathological changes directed towards control. It could be conducted that administration of (NNC) have potential to exert therapeutic effect against mammary carcinogenesis
https://bvmj.journals.ekb.eg/article_53537_eee2998d229013f8bc6dd950d49137b6.pdf
2018-03-01
117
131
10.21608/bvmj.2018.53537
anthracene
Mammary Carcinogenesis
Lysine – cetrimonium
Omayma
Abou Zaid
omaymaabouzaid@yahoo.com
1
Biochemistry Department, Faculty of Vet. Med. Benha University, Egypt
AUTHOR
Mohamed
MahfouZ
2
Biochemistry Department, Faculty of Vet. Med. Benha University, Egypt
AUTHOR
Abdel Fatah
Badwi
3
Petrochemical Department, Egyptian Petroleum Research Institute , Cairo, Egypt
AUTHOR
Salma
Abd- El wahab
4
Biochemistry Department, Faculty of Vet. Med. Benha University, Egypt.
AUTHOR
ORIGINAL_ARTICLE
Bacteriological evaluation of minced meat with special reference to effect of thyme essential oil on it
Meat products are ideal medium for bacteria because of high moisture content, richness in nitrogenous compounds. Twenty five random samples of minced meat were collected from different supermarkets in, Menofia governorate. All collected samples were subjected to bacteriological examination for aerobic plate count, coliform Enterobacteriacea, Staphylococci count and detection of,staph .aureus organisms. Also twelve samples of minced beef were collected from different butcher shops in Menofia governorate were divided into untreated (control) and treated samples which homogenized with thyme oils in 0.5%, 1% and 1.5% concentrations. Each sample was analyzed bacteriological promptly during cold storage until spoilage for studying the antimicrobial efficiency of Thyme oil in minced beef. Mean value of Aerobic Plate Count, coliforms Enterobacteriaceae and Staphylococci count of the examined minced beef were 6.52x 104 0.27x 104, 7.8x102 0.15x102 , 5.82×102 ± 1.02×102. and 6.15×102 ± 1.36×102 respectively. Seven strains of Coagulase + ve S.aureus , and Staphylococcus epidermidis, were isolated while 2 strains of Staphylococcus saprophyticus , Staphylococcus intermedius Staphylococcus capitis and Micrococcispp .were isolated from examinad samples. In case of using thyme oil at the concentrations 0.5%, 1% and 1.5% the scores of sensory evaluation were 9,9 , 8, 6 ,5& 9 ,9, 8, 7,6 & 9,9,8,8 , 7after zero day, 1st day, 2nd day ,3rd day and 4th day of the cold storage period respectively, comparing to the scores of sensory evaluation in the control samples which were 9, 6,5,4 ,2 after zero day, 1st day, 2nd day ,3rd day and 4th day of the storage period respectively. At zero day, for all samples the initial counts of APC was 5.9x104+0.6x104. For control sample and treated samples with 0.5%,1%,1.5%of thyme oil ,the mean counts were 8.2x104+0.7x104, 2.1x105+1.6 x 105, 5.3x105+1.2x105, 1.5x106+0.6x1063.7x104+0.5x104, 3.2x104+0.7x104, 4.0x103+0.6x103, 2.1x103+1.8x103 & 2.5x104+0.4x104, 1.9x103+1.1x103 , 3.6x103+1.4x103 , 1.6x103+0.8x103 & 1.8x104+0.5x104 , 1.4x104+0.4x104 , 2.9x103+0.8x103, 1.4.x103+1.2x103 cfu/g at1st day,2nd day,3rdday and4th day respectively. Reduction % of APC were37.28% , 45.76%, 93.22%, 96.44%&57.63%, 96.78%, 93.90% ,97.29%&69.49%, 76.27%,95.08%, 97.63% at 1st day,2nd day,3rdday and4th day respectively. BENHA VETERINARY MEDICAL JOURNAL, VOL. 34, NO.1:132 -151 , MARCH, 2018 BENHA UNIVERSITY FACULTY OF VETERINARY MEDICINEBacteriological evaluation of minced meat with special reference to effect of thyme essential oil on it 133 At zero day, for all samples, the initial count of Enterobacteriaceae was 7.1x103+0.6x103.. For control sample and treated samples with0.5%,1%,1.5%of thyme oil ,the mean counts were 7.5x104+0.7x104, 8.1x104+1.1x104, 9.4x104+1.5x104,3.1x105+1.2x105&5.7x103+0.5x103,5.3x4x103, 4.1x103+0.4x103,3.8x103+1.8x103&4.8x103+0.4x103,4.3x103+1.1x103, 3.5x103+1.4x103,,2.9x103+0.8x103&4.0x103+0.5x103,3.2x103+0.4x103, 2.3x103+0.8x103, 1.4.x103+1.2x103 cfu/g in the 1st day ,2nd day 3rdday and 4th day respectively . Reduction% of Enterobacteriaceae count were 19.72% ,25.35% ,42.25% ,46.48% &32.39%,39.44%,50.80%,59.15%&43.66%,54.93%,67.61%,80.29 at 1st day,2nd day,3rdday and4th day respectively. At zero day, for all samples the initialcountsofcoliformwas6.6x103+0.6x103 For control samples and treated samples with 0.5%,1%,1.5%of thyme oil , the mean counts were 4.2x104+0.7x104 , 8.9x104+1.6 x 104 , 1.3x105+1.2x105 ,2.5x105+0.6x105 &5.1x103+0.5x103 ,4.2x103+0.7x103 ,3.6x103+0.6x103, 5.6x102+1.8x102 &3.5x103+0.4x103, 2.8x103+1.1x103 , 2.3x103+1.4x103 , 6.8x102+0.8x102 & 2.6x103+0.5x103, 2.0x103+0.4x103, 1.5x103+0.8x103 , 6.4.x102+1.2x102 cfu/g. at 1st day,2nd day,3rdday and 4th day respectively. Reduction%ofcoliformcountwere22.73%,36.36%,45.45%,91.52%&32.39%,46.97%,57.58%,89.70%&6 0.61%,69.70%,77.27%,90.30% at 1st day,2nd day,3rdday and 4th day respectively. At zero day, for all samples the initial counts of Staphylococci was 5.1x103+0.6x103.For control sample and treated samples with 0.5%,1%,1.5%of thyme oil , the mean counts were 8.2x104+0.7x104, 2.1x105+1.6x105,5.3x105+1.2x105,1.5x106+0.6x106&3.7x103+0.5x103, 3.0x103+0.7x103,2.2x103+0.6x103,6.2x102+1.8x102&2.4x103+0.4x103, 1.8x103+1.1x103,8.6x102+1.4x102,5.8x102+0.8x102&2.0x103+0.5x103, 1.5x103+0.4x103, 7.8x102+0.8x102, 5.3.x102+1.2x102cfu/g in 1st day, 2nd day 3rdday and 4th day respectively. Reduction % of Staphylococci count were 27.45%,41.18%, 56.86% ,87.84% &52.94%, 64.71%, 83.14%, 88.61% & 60.78%, 70.59%, 84.71%, 89.61%, at 1st day, 2nd day , 3rdday and 4th day respectively . There was a significant antimicrobial effect of different concentrations of thyme oil on bacteriological state of minced beef.
https://bvmj.journals.ekb.eg/article_53538_160d538cd1fc559131c9b143c5fa382a.pdf
2018-03-01
132
151
10.21608/bvmj.2018.53538
minced beef
bacteriological profile thyme essential oil
Fahim
Shaltout
fahimshaltout@yahoo.com
1
Food hygiene Dep., Fac. Vet. Med. Benha Univ
AUTHOR
Nahla
Abou-Elroos
nahlaabou-elroos@yahoo.com
2
Animal Health Research “Shebin El- Kom branch
AUTHOR
Ahmed
Youness
3
Fairmont Nile City
AUTHOR
ORIGINAL_ARTICLE
Assessment of Heavy Metals in Cooked Meat and Chicken Meat
This study was conducted to evaluate the levels of heavy metals (lead , cadmium and copper)in cooked beef and chicken meat . The obtained results indicated that the mean values of lead concentration in the examined samples of boiled chicken thigh, boiled chicken breast, roasted chicken thigh, roasted chicken breast, boiled fore quarter beef and boiled hind quarter beef were 0.15± 0.01 , 0.12± 0.01 ,0.21± 0.02 ,0.16± 0.01 ,0.09± 0.01 and 0.13±0.01 mg/kg wet weight, respectively. While the mean values of cadmium concentration in the examined samples of boiled chicken thigh, boiled chicken breast, roasted chicken thigh, roasted chicken breast, boiled fore quarter beef and boiled hind quarter beef were 0.09± 0.01 , 0.06± 0.01 ,0.14± 0.01 ,0.10± 0.01 ,0.05± 0.01 and 0.08± 0.01 , respectively Furthermore, and that the mean values of copper concentration in the examined samples of boiled chicken thigh, boiled chicken breast, roasted chicken thigh, roasted chicken breast, boiled fore quarter beef and boiled hind quarter beef were 2.28± 0.35 , 1.47± 0.22 ,2.59± 0.46 ,2.06± 0.31 ,1.47± 0.19 and 1.60±0.23 mg/kg wet weight, respectively, The public health importance of the heavy metals and the recommended points were discussed.
https://bvmj.journals.ekb.eg/article_53542_b194fea347497c2217f4e0eca1333f7d.pdf
2018-03-01
152
161
10.21608/bvmj.2018.53542
Mohamed
Mohamed
mohamed@yahoo.com
1
Animal Health Research Institute, Benha branch
AUTHOR
Fatin
Hassanen
2
Meat Hygiene Dept., Faculty of Veterinary Medicine, Benha University
AUTHOR
Heba
Abozaid
3
Food hygiene Dept., Animal Health research Institute
AUTHOR
ORIGINAL_ARTICLE
In-vitro studies on antiviral effects of Galaxaura elongata marine algae on white spot syndrome virus
White spot syndrome virus is currently the most devastating viral pathogen threatening the shrimp farming industry worldwide. Studying efficiency of Galaxaura elongata as red marine algae on that shrimp virus was the aim of this work. Cells culture from lymphoid organ of Penaeid semesulacatus were established and when confluent cell monolayer was formed, the cell were infected with 0.1ml virus of titer 106.4 TCID50/ml mixed with (5, 10, 20 µl) algal extract. Data cleared algal extract of G. elongata has antiviral effect to WSSV where it results in reduction of virus titer 1.3 logs at concentration 5 µl/well, 2.2 logs at concentration10 µl/well and the highest reduction was at concentration 20 µl/well which was (2.5 log10TCID50). Algal extract was reduced CPE of infected cells in the attachment assays by 55, 70 and 75% consecutively and in the trypsin inhibition assay a significant activity was determined for 10 µl extract concentration. So, it can be suggested that using of G. elongata as red algae has vitro effect against WSSV replication.
https://bvmj.journals.ekb.eg/article_54231_cd8a59bcf2736d410ca6d955aba3023e.pdf
2018-03-01
162
171
10.21608/bvmj.2018.54231
White spot syndrome virus
Galaxaura elongata and shrimp
Ahmed
Abdel-Wahab
1
Central Laboratory for Aquaculture Research, Abbassa, Agriculture Research Center
AUTHOR
ORIGINAL_ARTICLE
Clinical, Biochemical and Radiographic Alterations in Kittens with Experimental Induced Nutritional Secondary Hyperparathyroidism
Nutritional secondary hyperparathyroidism (NSHPT) is a metabolic disorder that is caused by excessive phosphate in diets, insufficient calcium, or both. This study was carried out on 20 male kittens with 2-3 month of age. The NSHPT was induced in 10 kittens by feeding solely on heart beef meat for 9 weeks. The other 10 kittens were fed on commercial diet as control. Clinically, affected kittens were unable to stand or walk then recumbent with bone abnormalities lameness and lately loss their body weight. Serum biochemical changes showed significant (P<0.05) increase in CPK, ALP, phosphorus and 1,25 dihydroxyVit.D3, but showed significant (P<0.05) decrease in 25(OH)Vit.D3, total protein, albumin, globulin, blood urea nitrogen, creatinine, calcium, magnesium, copper and zinc in cats with induced NSHPT compared to control.Radiographic changes in cats with induced NSHPT showed generalized decrease in bone opacity which affects entire skeleton (osteoporosis); cortices become thin and faint with decreased bone to soft tissue contrast. It was concluded that NSHPT is associated with clinical, biochemical and radiographic changes in young growing kittens. It is recommended to give kittens a balanced diet especially in Ca and P during the first 9 weeks of life
https://bvmj.journals.ekb.eg/article_54232_1684c5b5fe63814e531e616d82b11c21.pdf
2018-03-01
172
181
10.21608/bvmj.2018.54232
Kittens
NSHPT
Radiography
M.M
Ghanem
1
Animal Medicine Department, Faculty of Veterinary Medicine, Benha University.
AUTHOR
S.F
EL Fakhrany
2
Menufia Veterinary Medicine Directorate
AUTHOR
Y.M
Abd El-Raof
3
Animal Medicine Department, Faculty of Veterinary Medicine, Benha University
AUTHOR
H.M
El-Attar
4
Animal Medicine Department, Faculty of Veterinary Medicine, Benha University
AUTHOR
ORIGINAL_ARTICLE
Evaluation of Four Treatment Protocols on Experimentally Induced Nutritional Secondary Hyperparathyroidism in Kittens
The aim of this study was to compare the effect of different treatment in cat models of induced nutritional secondary hyperparathyroidism (NSHPT) by using chicken's eggs hell compared to other conventional treatments. The NSHPT was induced experimentally in 20 rapidly growing kittens by feeding a diet consisted of only beef heart meat (low Ca-high P) for 9weeks. The clinical signs of NSHPT in kittens included disturbance in locomotion manifested by reluctance to move, posterior lameness, and uncoordinated gait. The diseased kittens were divided into four equal groups (n=5), each group was treated with a different protocol. Treatment of affected kittens was achieved by eggshell (G4) that was compared to three treatment protocols including combined Ca- vitamin D injection. (G1), oral Ca alone (G2) and oral Ca, Mg, and Zn (G3). The clinical symptoms and biochemical analysis were monitored at day 1, 3, 5 and 7 after treatment. The PTH and 1.25 (OH) 2 Vit.D3were gradually reduced starting from the 1st day post treatment in eggshell treated group compared to other treatment groups. The eggshell treatment induced changes in clinical signs and biochemical parameters that were comparable to the other three groups. Based on these results, it was suggested that feeding egg shell solution could be used as an economic source of Caasan alternative treatment for NSHPT in cats.
https://bvmj.journals.ekb.eg/article_54233_57d71361f90a38e2f1baacb0a76280a9.pdf
2018-03-01
182
194
10.21608/bvmj.2018.54233
calcium
Egg shell
Kittens
NSHPT
Vitamin D
M.M
Ghanem
1
Animal Medicine Department, Faculty of Veterinary Medicine, Benha University
AUTHOR
S.F
EL-Fakhrany
2
Menufia Veterinary Medicine Directorate
AUTHOR
Y.M
Abd El-Raof
3
Animal Medicine Department, Faculty of Veterinary Medicine, Benha University.
AUTHOR
H.M
El-Attar
4
Animal Medicine Department, Faculty of Veterinary Medicine, Benha University
AUTHOR
ORIGINAL_ARTICLE
Pathological Studies on Foot and Mouth disease at Kaluobia Governorate
The aim of this study was to detect the pathological affections in various body organs in dead animals by foot and mouth disease (FMD) throughout Kaluobia Governorate, Egypt. The study included 882 clinically affected animals showing characteristic signs of foot and mouth disease including, 827 cattle, and 55 buffalo. The age of the examined animals ranged from 30 days to 3 years and they were of both sexes. The total mortality rates were 6.65% in cattle and 54% in buffalos. The most common clinical signs that appeared on examined cases represented by fever, depression, recumbency, lameness, ropy salivation, ptyalism with protrusion of the tongue. Moreover, in young calf's sudden death was the only observed clinical signs. Histopathological examination of both young calves (1-2 months old) and aged calves (12-24 months old), revealed extensive inflammatory and necrotic lesions in the myocardium, lung, liver, kidney, spleen and intestine. Viral isolation from tongue epithelium and molecular identification revealed the presence of FMD virus 'type O'.
https://bvmj.journals.ekb.eg/article_54234_3bbe9378e8ed07a792bf168333970c69.pdf
2018-03-01
195
208
10.21608/bvmj.2018.54234
FMD
Pathology
Cattle and buffalos
Kaluobia
Mahmoud
Gab-Allah
1
Pathology department, Faculty of Veterinary Medicine, Benha University, Egypt
AUTHOR
Abdel-Baset
El-Mashad
2
Pathology department, Faculty of Veterinary Medicine, Benha University, Egypt
AUTHOR
Shawky
Moustafa
3
Pathology department, Faculty of Veterinary Medicine, Benha University, Egypt
AUTHOR
Ibrahim
El-maghraby
4
Pathology department, Faculty of Veterinary Medicine, Benha University, Egypt
AUTHOR
ORIGINAL_ARTICLE
Effect of some Dietary Supplementation on Economic Efficiency of Laying Japanese Quail
This work was conducted to study the effect of sun dried tomato pomace (SDTP) with or without enzymes supplementation on economic efficiency of laying Japanese quail. A total of 144 forty-five days-old female Japanese quails were used. The hens were randomly allocatted into 6 groups (24 hens per each group which subdivided into 3 replicates each one contains 8females.). Group 1 was fed on the basal diet (BD) (control), group 2 were fed on the BD containing AveMix® 02 CS enzyme 0.2g/kg diet. While hens of group 3 were fed BD containing 2.5% SDTP). Group 4 received BD containing 2.5% SDTP with AveMix® 02 CS enzyme. Group 5 received BD containing 5% SDTP. Group 6 received BD containing 5% SDTP with AveMix® 02 CS enzyme. From the obtained results it was observed that, the increasing 2.5% SDTP resulted in an increase of egg mass by 11.08%. In addition to, enzymes addition leads to increasing weight of egg mass and decrease of TC by 0.82%.
https://bvmj.journals.ekb.eg/article_54237_968a7ab952766ea3ea75538cf514606b.pdf
2018-03-01
209
218
10.21608/bvmj.2018.54237
Japanese quail
tomato pomace
Enzymes
economic efficiency
Seham
Shehata
1
Department of Animal Wealth Development, Faculty of Veterinary Medicine, Benha University
AUTHOR
Eman
Kamel
2
Department of Animal Wealth Development, Faculty of Veterinary Medicine, Benha University
AUTHOR
Mohammed
Abo-Salem
3
Department of Forensic Medicine and Toxicology, Faculty of Veterinary Medicine, Benha University
AUTHOR
Sanad
Atallah
4
Department of Animal Husbandry and Animal Wealth Development, Faculty of Veterinary Medicine, Alexandria University
AUTHOR
ORIGINAL_ARTICLE
Effect of Some Dietary Supplementation on Economic efficiency of growing Japanese Quails
This work was conducted to study the effect of sun dried tomato pomace (SDTP) with or without enzymes supplementation economic efficiency of Japanese quail. A total number of 306 seven- days-old quail chicks were used. The quail chicks were randomly allocated into 6 groups (51 unsexed chicks per group). Group 1 was fed on the basal diet (BD) without enzymes supplementation (control), quail chicks of group 2 were fed on the BD containing AveMix® 02 CS enzyme 0.02g/kg diet. While quail chicks of group 3 were fed BD containing 2.5% DTP). Group 4 received BD containing with 2.5% SDTP with Ave Mix® 02 CS enzyme. Group 5 received BD containing 5% SDTP. Group 6 received BD containing 5% SDTP with Ave Mix® 02 CS enzyme. Our results clarified that, the increasing (5%) SDTP to diet resulted in an increase of final weight by 29.36%. Also, increasing body weight resulted in an increase of total cost by 0.55% in case of 2.5% SDTP group.
https://bvmj.journals.ekb.eg/article_54243_4ae59a61fa3ced460d178e1f86442b0d.pdf
2018-03-01
219
231
10.21608/bvmj.2018.54243
Japanese quail
dried tomato pomace and Economic efficiency
Seham
Shehata
1
Department of Animal Wealth Development, Faculty of Veterinary Medicine, Benha University
AUTHOR
Eman
Kamel
2
Department of Animal Wealth Development, Faculty of Veterinary Medicine, Benha University
AUTHOR
Mohammed
Abo-Salem
3
Department of Animal Husbandry and Animal Wealth Development, Faculty of Veterinary Medicine, Alexandria University
AUTHOR
Sanad
Atallah
4
Department of Animal Husbandry and Animal Wealth Development, Faculty of Veterinary Medicine, Alexandria University
AUTHOR
ORIGINAL_ARTICLE
Study on Aflatoxin Residues in some Meat Products and their Control by Probiotics
Mould not only causes deterioration of food and feed, but also adversely affects the health of man and animals since it is capable of producing toxic metabolites known as mycotoxins causing food poisoning and liver cancer. Thus, creating awareness about aflatoxin residues and their control in food is of great importance for public health. From this point of view, this study was designed to evaluate total aflatoxin residues and their control by probiotics. A total of 100 random samples of meat products represented by beef burger, sausage, minced meat and luncheon (25 of each), were collected from different super markets in Cairo governorate. The obtained results revealed that the total aflatoxin residues were higher in luncheon (1.63±0.32 ppb) with the highest prevalence (88%). Also, the effect of probiotics on the reduction of aflatoxin residues in naturally contaminated minced meat sample was studied. Two probiotic strains (Lactobacillus acidophilus and Bifidobacteriumlactis) could be able to cause gradual reduction in total aflatoxin residues up to 88% and 98.3%, respectively of total aflatoxin residues within 8 days of experiment.
https://bvmj.journals.ekb.eg/article_54244_f5fa7444b38168ef6d25e5ae09a3846b.pdf
2018-03-01
232
241
10.21608/bvmj.2018.54244
Aflatoxin residues
AFT
AFB1
Lactobacillus acidophilus
Bifidobacteriumlactis and ELISA
Hemmat
Ibrahim
hemat.mohamed@fvtm.bu.edu.eg
1
Benha University Egypt. -Food Hygiene and Control Department, Faculty of Veterinary Medicine
AUTHOR
Reham
Amin
rehamnour2007@yahoo.com
2
Benha University Egypt. -Food Hygiene and Control Department, Faculty of Veterinary Medicine
AUTHOR
Khalid
Tolba
3
Animal Health Research Institute, Agriculture Research center,Dokki, Egypt.
AUTHOR
Amira
Elokle
4
Animal Health Research Institute, Agriculture Research center,Dokki, Egypt
AUTHOR
ORIGINAL_ARTICLE
The Effect of Probiotics on Staphylococcus Aureus and E. Coli in Minced Meat
Nowadays, all interested parties in the field of food safety are shifted to use natural food preservatives instead of chemical ones which proved to have many draw backs either on human health or food composition. The present study was conducted to study the effect of using two probiotic strains (Lactobacillus acidophilus and Bifidobacteriumlactis) individually on the growth and survival of some food-borne pathogens represented by Staphylococcus aureus and Escherichia coli experimentally inoculated separately into fresh minced beef, previously gamma irradiated using 5 KGy to be sure that samples were free from microorganisms under investigation during storage at 4°C.The obtained results revealed that the effect of Lactobacillus acidophilus on the reduction of Staph.aureus count was almost identical to the effect of Bifidobacteriumlactis. Moreover, Staph.aureus growth persisted till the 6th day of storage, while the organism was completely inhibited at the 8th day of the experiment. Bifidobacteriumlactis was more effective in reducing E. coli count through the 8 days of experimental study than Lactobacillus acidophilus. Overall, E. coli could persist till the end of the experimental period in the presence of both probiotics. The maximum reduction % of E. coli count reached 2.0 log10cfu/g (46.95%) in experimental samples using Bifidobacteriumlactis.
https://bvmj.journals.ekb.eg/article_54246_004f74010558f9b7ac70d02ef56ab195.pdf
2018-03-01
242
253
10.21608/bvmj.2018.54246
Minced meat
probiotics
Staph. aureus
E. coli
Lactobacillus acidophilus
Bifidobacteriumlactis and Radiation
Hemmat
Ibrahim
hemat.mohamed@fvtm.bu.edu.eg
1
Benha University Egypt. -Food Hygiene and Control Department, Faculty of Veterinary Medicine
AUTHOR
Reham
Amin
rehamnour2007@yahoo.com
2
Benha University Egypt. -Food Hygiene and Control Department, Faculty of Veterinary Medicine
AUTHOR
Khalid
Tolb
3
Dokki, Egypt. Animal Health Research Institute, Agriculture Research center
AUTHOR
Amira
Elokle
4
Dokki, Egypt. Animal Health Research Institute, Agriculture Research center
AUTHOR
ORIGINAL_ARTICLE
Prevalence of Escherichia Coli in Fish Obtained from Retail Fish Markets in Gharbia Governorate, Egypt
A total of 100 random samples of fresh and marine fishes represented by Tilapia niloticus, Mugil cephalus, Sardine and Barboni (25 0f each) were collected from the different fish markets located in Gharbia governorate. E. coli was identified by standard microbiological, biochemical tests, and further confirmed by multiplex PCR. The faecal coliform loads in fish samples were assessed. The bacterium was detected in 8(32%), 6(24%), 4(16%) and 3(12%) of the examined samples of Oreochromis niloticus, Mugil cephalus, Sardine and Barboni respectively. The accepted fish samples were 17 (68 %), 19 (76 %), 21 (86 %) and 22 (88 %) of the examined samples of O. niloticus, Mugil cephalus, Sardine and Barboni respectively while the unaccepted samples were 8 (32 %), 6 (24 %), 4 (16 %) and 3 (12 %) of the examined samples of O. niloticus, Mugilcephalus, Sardine and Barboni respectively according to Egyptian standards (E.S 3494/2005). the results cleared that, the main isolates include O26: H11; O44: H18, O86: H; O91: H21; O103: H2; O119: H6; O121: H7; O124: H; O128: H2; O146: H21 and it isolated mainly from O. niloticus, Mugil cephalus, then Sardine and Barboni. The work was aimed to study prevalence of Escherichia coli contamination in fish sold in different fish markets located in Gharbia governorate.
https://bvmj.journals.ekb.eg/article_54248_864079fea24635cd61712dcd2dae80d7.pdf
2018-03-01
254
260
10.21608/bvmj.2018.54248
Escherichia coli
Oreochromis niloticus
Mugil cephalus
Sardine
Barboni
Saad
M.S
1
Benha University Egypt. -Food Hygiene and Control Department, Faculty of Veterinary Medicine
AUTHOR
M.A
Hassan
2
Laboratory for Aquaculture Research, Agriculture Research Center, EgyptCentral
AUTHOR
F.S.
Hassnien
3
Benha University Egypt. -Food Hygiene and Control Department, Faculty of Veterinary Medicine
AUTHOR
M.M
Abdel-Aal
4
Laboratory for Aquaculture Research, Agriculture Research Center, Egypt Central
AUTHOR
A.H
Zakar
5
Laboratory for Aquaculture Research, Agriculture Research Center, EgyptCentral
AUTHOR
S.A
Elshfey
6
Laboratory for Aquaculture Research, Agriculture Research Center, Egypt Central
AUTHOR
ORIGINAL_ARTICLE
Enterobacteriaceae in Some Fresh and Marine Fish
A total of 100 random samples of fresh and marine fishes represented by Tilapia niloticus, Mugil cephalus, Sardine and Barboni (25 0f each) were collected from the different fish markets located in Gharbia governorate. All collected samples were subjected to bacteriological examination for isolation and identification of Enterobacteriaceae. The results revealed that the total Enterobacteriaceae counts in the examined samples of fresh and marine fish were varied from 1.4×103 to 5.7×104 with a mean value ( 1.9×104 )cfu/g for Oreochromis niloticus ,7.0×102 to 2.40 ×104 with a mean value (9.06×103 )cfu/g for Mugil cephalus , 4.0×102 to 8.9×103 with a mean value, 4.25×103cfu/g for Sardine , 1.0×102 to 3.30 ×103 with a mean value ( 1.18×103 )cfu/g for Barboni. Also, Salmonella species were recovered from 24 %, 20 %, 8% and 8 % of the examined samples of O. niloticus, Mugil cephalus, Sardine and Barboni, respectively. Enterobacteriaceae count, Coliform count and the isolated Salmonella organisms as well as the public health significance were discussed and the prophylactic measures to reduce contamination in fish were recommended The work was aimed to study occurrence of Enterobacteriaceae in fish sold in different fish markets located in Gharbia governorate.
https://bvmj.journals.ekb.eg/article_54250_9ffac8c88ed196e8af1d2c4a4f5fb4d1.pdf
2018-03-01
261
268
10.21608/bvmj.2018.54250
Enterobacteriaceae
Oreochromis niloticus
Mugil cephalus
Sardine
Barboni
Salmonella
Saad
Saad
1
Food Control Department. Faculty of Veterinary Medicine, Benha University
AUTHOR
mohamed
Hassan
2
Food Control Department. Faculty of Veterinary Medicine, Benha University
AUTHOR
Faten
Hassnien
3
Food Control Department. Faculty of Veterinary Medicine, Benha University
AUTHOR
M
Abdel-Aal
4
Central Laboratory for Aquaculture Research, Agriculture Research Center, Egypt
AUTHOR
A
Zakar
5
Central Laboratory for Aquaculture Research, Agriculture Research Center, Egypt.
AUTHOR
S
Elshfey
6
Central Laboratory for Aquaculture Research, Agriculture Research Center, Egypt.
AUTHOR
ORIGINAL_ARTICLE
Bacteriological evaluation of fresh and cooked meat meals served at a university hostel restaurant
A total of 90 random sample of fresh and cooked meat (boiled) (45 of each) were collected from university student hostel, and examined bacteriologically to detect its safety. Aerobic plate count (APC), Staphylococcal counts and Coliforms count were examined, Isolation and identification to E.coli. The obtained results in this study indicated that the mean value of APC was 6.32×105± 1.17×105 cfu/g for fresh meat and 1.08×105± 0.24×105 cfu/g for cooked meat; furthermore APC was detected in 75.5% in fresh meat according to EOS (2008) and 86.7% in cooked meat according to Center for Food Safety 2014. The obtained results indicated that the mean values of Staphylococcal count and coliforms in examined samples were 1.10×103± 0.21×103 and 9.95×103± 2.03×103 (cfu/g) for fresh meat and 7.78×102± 1.64×102 and 2.14×103± 0.51×103 (cfu/g) for cooked meat. Incidence of E.coli from fresh meat was 28.9% and 24.4% from cooked meat. These results indicate that fresh meat had higher significant results than cooked meat due to cooked meat were subjected to heat treatment that lead to killing of microorganisms, moreover, presence of E.coli in cooked meat indicated post heat treatment infection.
https://bvmj.journals.ekb.eg/article_54251_b246574814edf791ee5b4b2ee227089d.pdf
2018-03-01
269
276
10.21608/bvmj.2018.54251
Meat meals
APC
Staphylococcal count
Coliforms count and E.coli
mohamed
Hassan
1
Food Control Department. Faculty of Veterinary Medicine, Benha University
AUTHOR
Rasha
Elsabagh
rasha.alsbagh@fvtm.bu.edu.eg
2
Food Control Department. Faculty of Veterinary Medicine, Benha University
AUTHOR
Nesreen
Eleiwa
nesreenwow@gmail.com
3
Food Control Department, Animal Health Research Institute, Tanta Branch
AUTHOR
Hanady
Zohdy
4
Food Control Department, Animal Health Research Institute, Tanta Branch
AUTHOR
ORIGINAL_ARTICLE
Chrysin regulates NAD(P)H:quinoneoxidoreductase 1 and inflammatory mediators in rats exposed to smoking and thioacetamide
Tobacco smoking and hepatic damage causes oxidative stress in humans and underlay numerous chronic degenerative diseases. Heavy smoking yields toxins which induce necroinflammation and increase the severity of hepatic lesions. Accordingly, the main object of this study to investigate the effect of chrysin against thioacetamide (TAA)-induced liver injury in rats exposed to tobacco smoke in combination. The study was carried out by investigating the oxidative stress biomarkers (glutathione peroxidase, SOD, catalase, paroxynase-1 and NAD (P) H: quinoneoxidoreductase 1 activities), inflammatory markers (IL-6 and IL-8 levels and gene expressions of NF-κB and VEGF) as well as histopathogical examination. It was found a significant reduction in activities of antioxidant enzymes concomitant with cytokines elevation; (IL-6 and IL-8) in rats exposed to tobacco smoke in combination with (TAA) treatment compared with normal control. Additionally, the transcript levels of (NF-κB and VEGF) genes were significantly higher in the rats exposed to tobacco smoke in combination with (TAA) treatment compared with normal control. However, treatment with chrysin, the levels of these parameters were significantly decreased which demonstrates the ameliorative effect of chrysin against (TAA) induced liver injury in rats exposed to tobacco smoke in combination. Histological investigations revealed that chrysin gave more preventive effect against thioacetamide (TAA)-induced liver injury in rats exposed to tobacco smoke. In conclusion, chrysin modulated the damage induced by exposure to tobacco smoking and hepatic disease
https://bvmj.journals.ekb.eg/article_54252_5a2a195760095a317bbf8c18f368e7c0.pdf
2018-03-01
277
288
10.21608/bvmj.2018.54252
Thioacetamide (TAA)
glutathione peroxidase (GPx)
superoxide dismutase (SOD)
catalase (CAT)
paroxynase -1
NAD P H oxidase
quinoneoxidoreductase 1 activities
Interleukin 6 (IL-6)
interleukin 8 ( IL-8)
nuclear factor-kappa B (NF-κB)
vascular endothelial growth factor (VEGF)
Omayma
Abozaid
1
Biochemistry Department. Faculty of Veterinary Medicine, Benha University
AUTHOR
Sawsan
El-Sonbaty
2
Radiation microbiology Department, National Center for Radiation Research and Technology, Atomic Energy Authority, Cairo, Egypt
AUTHOR
Ateff
Ghareeb
3
Biochemistry Department. Faculty of Veterinary Medicine, Benha University
AUTHOR
ORIGINAL_ARTICLE
Field Evaluation of the prepared combined equine influenza, equine herpesvirus-1 and tetanus toxoid vaccine in horses
The use of vaccines in the prevention and control of infectious diseases in the horse is a widely accepted practice. A combined inactivated EIV, EHV-1 and tetanus toxoid vaccine adjuvanted with alhydrogel and saponin was evaluated in horses. Two groups of horses, group A was inoculated with two doses of the combined vaccine one month apart and revaccinated at 6 months and group B was kept as control. The immune response of vaccinated horses against EI, EHV-1 and tetanus was evaluated by measuring Haemagglutination Inhibition (HI), Enzyme linked immunosorbent assay (ELISA), Complement Fixation (CF) and toxin neutralizing antibody titers (TN). Vaccinated horses had high antibody titers, above the level for clinical protection against influenza and tetanus until 12 months and 6 months for EHV-1 after the third vaccination. A vaccination scheme consisting of three doses with combined inactivated EI, EHV-1 and TT vaccine adjuvanted with saponin and alhydrogel, two doses at one-month interval and Revaccination after 6 months, Followed by alternating vaccinations with monovalent inactivated EHV-1vaccine and combined inactivated EI- EHV& TT vaccine every 6 months is proposed to maintain maximal protection against equine influenza and herpesvirus and tetanus infections.
https://bvmj.journals.ekb.eg/article_54253_5cf16b554dc39f3f23a066ba06e6f9a6.pdf
2018-03-01
289
299
10.21608/bvmj.2018.54253
EIV
EHV-1
tetanus toxoid
Horses
vaccine
immunity
Mohamed
Bayoumi
1
Equine Viral Disease Research Department, Veterinary Serum and Vaccine Research Institute, Abbasia
AUTHOR
Abdelfattah
Selim
abdelfattah.selim@fvtm.bu.edu.eg
2
Animal Medicine Department, Faculty of Veterinary Medicine, Benha University
AUTHOR
Abdelmoneim
Moustafa
profmoneim@yahoo.com
3
Animal Medicine Department, Faculty of Veterinary Medicine, Benha University
AUTHOR
Nashwa
Madkour
4
Equine Viral Disease Research Department, Veterinary Serum and Vaccine Research Institute, Abbasia
AUTHOR
H
El-Helw
5
Anaerobic Bacterial Vaccine Department, Veterinary Serum and Vaccine Research Institute, Abbasia
AUTHOR
ORIGINAL_ARTICLE
Influence of pesticides pollution on mitochondrial 12S rRNA gene in cultured Mugil capito from different regions at Kafr El-Shiekh governorate, Egypt
The aim of this study was to evaluate the impacts of pesticides pollution on the sequence of mitochondrial 12S rRNA in liver tissues of Mugil capito that obtained from three different regions at Kafr El Sheikh Governorate, Egypt. The results revealed ten SNPs between three sequences of different regions after alignment of these sequences submitted under accession numbers (MF817450.1), (MG210582.1) and (MG210583.1) with sequence with accession number (KU681005.1). Overall, the pesticides pollution has able to happen mutation in the mitochondrial gene.
https://bvmj.journals.ekb.eg/article_54254_04af8c348554ede32de42d28462dfd75.pdf
2018-03-01
300
304
10.21608/bvmj.2018.54254
Mugil capito
Pesticides
SNPs
12S rRNA gene sequence
Afaf
Abd El Megid
1
Department of Biochemistry. Faculty of Veterinary Medicine, Benha University
AUTHOR
Mohammad
Abd Al Fatah
2
Department of Biochemistry. Faculty of Veterinary Medicine, Benha University
AUTHOR
Amel
El Asely
amel.alaasly@fvtm.bu.edu.eg
3
Department of of Fish Diseases and Management. Faculty of Veterinary Medicine, Benha University
AUTHOR
Yakout
El Senosi
4
Department of Biochemistry. Faculty of Veterinary Medicine, Benha University
AUTHOR
Mahmoud
Moustafa
5
Department of of Genetics and Genetic Engineering. Faculty of Veterinary Medicine, Benha University.
AUTHOR
ORIGINAL_ARTICLE
Hygienic Considerations of Pathogenic Escherichia Coli Contamination on Cattle Carcass Surfaces in Egypt
A total of 100 random samples were collected from surfaces of cattle carcasses in EL-Shouhada and Birket El –Sabaa Abattoirs in Menofyia governorate (50 of each).The results showed that the mean E. coli counts were 5.44×102 and 7.99×102 cfu/cm2 in EL- Shouhada and Birket EL-Sabaa abattoirs, respectively. Also, the incidence of E .coli isolated from cattle carcass surfaces in EL-Shouhada and Birket El-Sabaa abattoirs were 26% and 28%, respectively. The isolated serotypes of E. coli were EHEC as O111 : H2 (8%) and O26 : H11 (2%), EPEC as O15 : H4 (2%), O55 : H7 (2%), O114 : H4 (4%) and O146 : H21(2%), ETEC as O128 : H2 (2%) and O125: H21 (2%) and EIEC as O124 (2%) in EL- Shouhada abattoirs, while in Birket EL-Sabaa abattoir were EHEC as O111 : H2 (6%) , O26 : H11 (8%) and O103 : H4 (2%), EPEC as O55 : H7 (2%) and O91 : H21(2%) ETEC as O128: H2 (6%) and EIEC as O124 (2%). Findings of multiplex PCR showed that eaeA (intimin) gene was detected in (O91:H21 and O114:H4), hlyA (haemolysin) gene was detected in (O125:H21), stx1 gene was not detected in the all isolated E. coli serogroups, but stx2 gene was detected in (O26:H11 and O114:H4).
https://bvmj.journals.ekb.eg/article_54255_582fd73c3bd2f40aa34cbe2580f36462.pdf
2018-03-01
305
313
10.21608/bvmj.2018.54255
Escherichia coli
Cattle Carcass
Menofyia governorate
Saad
Saad
1
Food Control Department. Faculty of Veterinary Medicine, Benha University
AUTHOR
Amani
Salem
amanisalem@yahoo.com
2
Food Control Department. Faculty of Veterinary Medicine, Benha University
AUTHOR
Shaimaa
Nada
dr.shaimaanada@yahoo.com
3
Animal Health Research Institute “Shebine EL-Koom Branch
AUTHOR
Mona
Mohamed –
4
Animal Health Research Institute “Shebine EL-Koom Branch”.
AUTHOR
ORIGINAL_ARTICLE
Biochemical role of zinc oxide and propolis nanoparticles in protection rabbits against coccidiosis
Coccidiosis is a protozoan infection of animals which causes growth retardation and high mortality in rabbits. There are two forms of coccidiosis were recognized in rabbits; hepatic and intestinal coccidiosis. The hepatic coccidiosis is due to Eimeria stiedae cause severe damage for liver which leads to death. Nanotechnology is the most important technology in our life where the use of nanotechnology in medicine offers some exciting possibilities. So, this study uses zinc oxide nanoparticles as chemical therapy and propolis nanoparticles as natural therapy. Twenty male rabbits aged 1 - 2 months and weighting 1250 – 1400 gm was used. Animals were divided into 4 groups (5 animals per each): control negative group; uninfected male rabbits and saved healthy, control positive group; infected male rabbits by 40.000 sporulated oocyst of Eimeria spp. and not treated. Zinc oxide nanoparticles treated group; infected male rabbits by 40.000 sporulated oocyst of Eimeria spp. and treated orally with 10 mg ZnO-NPs/Kg body weight daily for 5 consecutive days. Propolis nanoparticles treated group; infected male rabbits by 40.000 sporulated oocyst of Eimeria spp. and treated orally with 100 mg propolis-NPs/Kg body weight daily for 7 consecutive days. Serum ALT, CRP, zinc, cholinesterase, plasma GR, SOD and CAT were measured in addition to; histopathological examination of liver was done. The results indicated that the serum ALT and CRP were increased but serum cholinesterase , zinc , plasma GR , SOD and CAT were decreased in infected group ,with treatment all parameters were improved , also examination of histological sections of livers from all treated groups did not detect any protozoal stage in their parenchyma nor bile ducts. Also, no or minimal papillary projection of epithelium of bile duct with decrease of peribiliary fibrosis could be seen in comparison control positive group. In conclusion, zinc oxide nanoparticles and propolis nanoparticles play a protective role against Eimeria infestation in rabbits.
https://bvmj.journals.ekb.eg/article_54256_92b1652bf98aad78be80bc502b9821c9.pdf
2018-03-01
314
328
10.21608/bvmj.2018.54256
coccidiosis
Nanotechnology
Propolis-NPs
ZnO-NPs
Afaf
Abd El Megid
1
epartment of Biochemistry. Faculty of Veterinary Medicine, Benha University.
AUTHOR
Mohamed
Khaled
2
Department of Biochemistry. Faculty of Veterinary Medicine, Benha University
AUTHOR
Mahmoud
Emam
3
Department of of Histology. Faculty of Veterinary Medicine, Benha University.
AUTHOR
Amira
Adel
4
Department of Biochemistry. Faculty of Veterinary Medicine, Benha University
AUTHOR
ORIGINAL_ARTICLE
The ameliorative effect of Vitamin C in experimentally induced colon cancer in rats
The present study was designed to investigate the ameliorative effect of vitamin C administration on serum alanine aminotransferase (ALT),aspartate aminotransferase(AST),Urea, Creatinine, Alphafetoprotein(AFP), Carcinoembryonic antigen (CEA) and carbohydrate antigen 19.9 (CA 19- 9), in addition to tumor necrosis factor alpha (TNF-α) , Cytochromep4502E1 and Caspase-9 gene expression in colon cancer induced by 1,2-dimethylhydrazine (DMH)in rats. Forty white female albino rats were divided into four equal groups (10 each). Group I(control group):received no drugs. Group II:(DMH group): rats injected subcutaneously with DMH (35 mg/kg body weight),twice a week for 5 consecutive weeks for colon cancer induction. Group III:(protective group): rats administrated vit C(200 mg kg-1 b. wt) orally and for 4 weeks before DMH injection and continued with vit C administration daily till the end of the experiment .Group IV:(treatment group):rats injected subcutaneously with DMH then administrated Vit C for 6 weeks till the end of the experiment. Blood and colon tissue samples were collected from all animal groups at the end of the experiment. The obtained results showed that DMH injection to rats significantly increased serum(ALT, AST, Urea, Creatinine, AFP, CEA, CA 19-9) and colon tissue TNF-α and CYP2E1while colon tissue caspase-9 gene wassignificantly decreasedwhen compared to control.Administration of Vit C significantly decreased serum (ALT, AST, Urea, Creatinine, AFP, CEA, CA 19-9) in addition to TNF-α and CYP2E1in colon tissue. How every, caspase-9 gene expression showed a significant increase. Histopathological examination of colon tissue edesquamation of the colon epithelium with heavy leukocytic infiltrationin DMH group. While administration of vitamin C in colon cancer induced rats showed mild destruction of the lining epithelium with mild leukocytic infiltration in addition to mild malignancy of epithelium when compared to DMH group. These results indicated the protective effect of Vit C against DMH induced colon cancer.
https://bvmj.journals.ekb.eg/article_54257_988a3ab38cf59279fb15e53f022c8fde.pdf
2018-03-01
329
343
10.21608/bvmj.2018.54257
vitamin c
DMH
Colon cancer
Caspase-9 gene expression
Cytochrome 2E1
Tumor necrosis factor –alpha
Omnia
Abdel-Hamid
1
Department of Biochemistry, Faculty of Veterinary Medicine, Benha University
AUTHOR
Abeer
Nafee
2
Department of Physiology, Faculty of Veterinary Medicine, Benha University
AUTHOR
Emam
M.A
3
Department of Histology, Faculty of Veterinary Medicine, Benha University
AUTHOR
Elshimaa
M.A
4
Department of Biochemistry, Faculty of Science, Ainshams University
AUTHOR
ORIGINAL_ARTICLE
Bacteriological and Molecular Identification of some Campylobacter Species in Broilers and their Macrolide Resistance Profile
Genus campylobacter including several species is of great importance that is considered among the major causative agents of acute diarrheal diseases in humans worldwide. The current study was carried out to determine the occurrence of thermotolerant campylobacters in broilers and to identify the macrolides resistance profiles of C. jejuni and C. coli isolates. A total of 568 samples (364 cloacal swabs and 51 of each breast meat, thigh meat, caecal part and neck skin) were collected from broiler chickens at slaughter age from local pluck shop outlets in Zagazig city, Sharkia Governorate, Egypt. The isolation rate of Campylobacter species from neck skin, breast meat, cloacal swabs, thigh meat and caecal parts samples was 25.5%, 27.5%, 29.3%, 31.4% and 41.2%, respectively. C. jejuni was isolated from cloacal swabs, skin, thigh meat, breast meat and caecal parts samples with the isolation rate of 55.3%, 53.8%, 43.7%, 50% and 80.9%, respectively. Forty-two campylobacter isolates (28 and 14 biochemically suspected C. jejuni and C. coli isolates, respectively) were confirmed molecularly depending on 23S rRNA gene. Furthermore, real time PCR targeting hipO gene specific for C. jejuni and glyA specific for C. coli were used. The molecularly confirmed isolates were evaluated they macrolides resistance pattern which revealed that all isolates were resistant to macrolides. Further studies on the mechanisms of macrolides resistance in campylobacters are essential.
https://bvmj.journals.ekb.eg/article_54483_518a737997a9d54889ed39ea4f178b41.pdf
2018-03-01
374
391
10.21608/bvmj.2018.54483
C. jejuni
C. coli
broiler
rtPCR
macrolide
Egypt
Ashraf
Abd El Tawab
ashrafabdeltawab@yahoo.com
1
Department of Bacteriology, Immunology and Mycology, Faculty of Veterinary Medicine, Benha University
AUTHOR
Ahmed
Ammar
ammar@yahoo.com
2
Microbiology Dep., Fac. Vet. Med. Zagazig University
AUTHOR
Heba
Ahmed
3
Zoonoses Dep., Fac. Vet. Med. Zagazig University
AUTHOR
Ahmed
Hefny
4
Veterinary Hospital, Fac. Vet. Med. Zagazig University
AUTHOR
ORIGINAL_ARTICLE
Detection of Staphylococcus aureus in some meat products using PCR technique
Staphylococcus aureus is frequently implicated in food borne illnesses. A total of 100 random samples of meat products represented by minced meat, beef burger, kofta and luncheon (25 of each) were collected from different supermarkets in Gharbia governorate. 25 grams from each sample were subjected to bacteriological examination for determination of Staphylococci count and S. aureus count, isolation and identification of S. aureus then molecular examination; using PCR technique as a confirmatory method for identification of S. aureus isolates. In addition, multiplex PCR was used for detection of classic enterotoxin genes (sea, seb, sec and sed) of S. aureus isolates. The mean values of Staphylococcal and S. aureus count /g were 3.41x103±0.58x103 and 9.35x102±2.04x102 for minced meat; 7.95x103±1.22x103 and 1.87x103±0.36x103 for beef burger; 2.12x104±0.48x104 and 3.72x103±0.51x103 for kofta and 9.06x102±2.15x102 and 4.29x102±0.67x102 for luncheon, respectively. S. aureus was detected in 36%; 52%; 64% and 12% of the examined samples of minced meat, beef burger, kofta and luncheon, respectively. The occurrence of enterotoxin genes was determined in 12 isolates (3 from each meat product). The incidence of sea was 2/3 (66.67%) in beef burger and 1/3 (33.33%) in kofta. The incidence of seb was 1/3 (33.33%) in luncheon only. The incidence of sea and sed was 1/3 (33.33%) in minced meat only. The incidence of sea, seb and sec was 1/3 (33.33%) in kofta only. Meat products are considered a good medium for the growth of Staphylococci and the production of toxins.
https://bvmj.journals.ekb.eg/article_54492_b5ce77a0829be82d0b9a0d179f36e407.pdf
2018-03-01
392
403
10.21608/bvmj.2018.54492
S. aureus
meat products
enterotoxin
PCR
Mohamed
Hassan
mohamed.hassan@fvtm.bu.edu.eg
1
Food Hygiene Dept. (Meat Hygiene), Fac.Vet. Med., Benha University PO 13736 Moshtoher, Benha, Egypt
AUTHOR
Reham
Amin
rehamnour2007@yahoo.com
2
Food Hygiene Dept. (Meat Hygiene), Fac.Vet. Med., Benha University PO 13736 Moshtoher, Benha, Egypt
AUTHOR
Nesreen
Eleiwa
nesreenwow@gmail.com
3
Food Hygiene Dept., Animal Health Research Institute, Tanta lab
AUTHOR
Hala
Gaafar
4
Veterinary doctor, Directorate of Vet. Med., El-Gharbia, Egypt
AUTHOR
ORIGINAL_ARTICLE
Bacteriological characterization of Salmonella species isolated from laying ducks
The current study was aimed to investigate the incidence and bacteriological characterization of Salmonella serovars in laying ducks. A total of 52 samples of laying ducks were collected from different farms in Kalubia governorate, Egypt. Samples were isolated from liver, spleen, ovaries, oviducts and intestine. Salmonella isolation revealed a total percentage of 2%; ovaries & oviducts revealed a high incidence among the examined samples (4%), followed by liver & intestine with incidence of 3 and 1%, respectively. The results revealed one isolate of Salmonella strain which subjected to biochemical and serological identification. The isolated Salmonella was identified as being a non-lactose fermenting, (NLFs) Gram negative rod-shaped organism, oxidase negative, catalase positive, indole and Voges Proskauer (VP) negative, methyl red and Simmons citrate positive, H2S producing and urea negative. The isolated Salmonella serotyped as S. Typhimurium O1, 4, [5], 12: i : 1, 2 at Serological Department of Animal Health Research Center. Antimicrobial sensitivity test was conducted on the isolated Salmonella Typhimurium which showed resistance to doxycycline, ampicillin, gentamycin, colistin, vancomycin and neomycin
https://bvmj.journals.ekb.eg/article_54497_ed8fa3c26c3def8cbaebb7e0f0d22577.pdf
2018-03-01
404
412
10.21608/bvmj.2018.54497
Salmonella typhimurium
Ducks
antibiotics
Ashraf
Abdeltawab
1
Bacteriology, Immunology and Mycology Dep., Fac. Vet. Med. Banha Univ.
AUTHOR
Ehab
El- Nahas
2
virology Dep., Fac. Vet. Med. Banha Univ
AUTHOR
Ahmed
Askora
3
Microbiology, Botany Dep., Fac. Science. Zagazig Univ.
AUTHOR
Hayam
Abdelaziz
4
Vet. Fac. Vet. Med. Banha Univ
AUTHOR
ORIGINAL_ARTICLE
Detection of Aerobic Spore Formers in Ready to Eat Fish
A total of 300 fish samples were collected from Giza and Cairo shops, supermarkets and restaurants. these samples were 150 raw fish samples of Tilapia, Mackerel and Sardine (50 for each) and 150 ready to eat fish samples of fried Tilapia, grilled Mackerel and grilled Sardine (50 for each). samples were subjected to bacteriological examination for detection of aerobic spore forming bacteria. The obtained results indicated that, the infection rate with aerobic spore formers in raw Tilapia, Mackerel and Sardine were 60 %, 70 % and 100 %, respectively. While in fried Tilapia, grilled Mackerel and grilled Sardine were 30%, 14% and 20 %, respectively. The highest count of aerobic spore forming bacteria in raw fish samples found to be high in raw Sardine (7 x 103cfu/gm) while the lowest one in raw Tilapia, Mackerel and Sardine were (1 x 102 cfu /gm) for all. In ready to eat samples the highest count found in fried Tilapia samples (6 x 102 cfu/gm). Four serotypes of aerobic spore formers recovered from raw fish; B.cereus , B. subtilis, B. lanchiniformis and B. macerans while in ready to eat fish the same serotypes were found besides B. mycoides
https://bvmj.journals.ekb.eg/article_54500_dfde5e2380a462464b34d3fd5d7e16f1.pdf
2018-03-01
413
419
10.21608/bvmj.2018.54500
aerobic spore forming bacteria
fish
ready to eat fish
F.S.
Hassanien
1
Food Hygiene Dep., Fac. Vet. Med., Benha Univ ,
AUTHOR
M.A.
Hassan
2
Food Hygiene Dep., Fac. Vet. Med., Benha Univ
AUTHOR
M.D
El-Hariri
3
Microbiology Dep., Fac. Vet. Med., Cairo Univ
AUTHOR
Eid
Sayed
4
Directorate of Veterinary Medicine, Giza
AUTHOR
ORIGINAL_ARTICLE
Incidence and toxigenic profile of Bacillus cereus in some fishes
A total of 300 fish samples were collected from Giza and Cairo shops, supermarkets and restaurants. These samples were 150 raw fish samples of Tilapia, Mackerel and Sardine (50 for each) and 150 ready to eat fish samples of fried Tilapia, grilled Mackerel and grilled Sardine (50 for each). Each sample was subjected to bacteriological and molecular examination for detection of Bacillus cereus and its toxins. The obtained results using PCR indicated that the incidence of B. cereus in raw Tilapia, Mackerel and Sardine were 24% (12/50) , 26% (13/50) and 40% (20/50) respectively ; while for hemolytic toxin was 75% (9/12) , 62% (8/13) and 60% (12/20) , non-hemolytic toxin was 25% (3/12) , 38% (5/13) and 40% (8/20), cytotoxin K was 16.5% (2/12), 15% (2/13) and 0 and Cereulide was 16.5% (2/12) , 7.5% (1/13) and 5% (1/20) in positive samples of B. cereus in raw Tilapia, Mackerel and Sardine respectively. In ready to eat Tilapia, Mackerel and Sardine the incidence of B. cereus were 20% (10/50) , 18% (9/50) and 30% (15/50) respectively ; while for hemolytic toxin were 07% (0/07) ,67% (6/9) and 73% (11/15) , non-hemolytic toxins were 30% (3/10) ,33% (3/9) and 27 % (4/15) in in positive samples of B. cereus in ready to eat Tilapia , Mackerel and Sardine respectively. In this study we didn’t detected any cytotoxin K or cereulide toxins genes in any of the ready to eat fish samples
https://bvmj.journals.ekb.eg/article_54503_da244c23cd784e1afe1839fa2e29480b.pdf
2018-03-01
420
429
10.21608/bvmj.2018.54503
F.S
Hassanien
1
Food Hygiene Dep., Fac. Vet. Med., Benha Univ
AUTHOR
M.A
Hassan
2
Food Hygiene Dep., Fac. Vet. Med., Benha Univ
AUTHOR
M.D
El-Hariri
3
Microbiology Dep., Fac. Vet. Med., Cairo Univ
AUTHOR
Eid
Sayed
4
Directorate of Veterinary Medicine, Giza.
AUTHOR
ORIGINAL_ARTICLE
Prevalence and molecular studies on Listeria monocytogenes isolated from chicken in El-Gharbia Governorate
Listeria monocytogenes is the causative agent of listeriosis and food-borne disease that can lead to meningitis and bacteremia. L. monocytogenes is the most pathogenic species in the genus Listeria. A total of 400 random samples of muscles, liver, spleen and kidneys (100 samples each) were taken from 100 diseased chickens. All samples were obtained from different poultry farms and markets in El- Gharbia Governorate to estimate the prevalence of Listeria monocytogenes in chicken. The bacteriological examination of the samples resulted; 53(13.25%) isolates as 11, 21, 8 and 13 from muscles, liver, spleen and kidneys respectively. The antimicrobial sensitivity test showed that the isolated L. monocytogenes were sensitive to sulfamethoxate-trimethoprim (91.6%) followed by ampicillin, gentamycin, vancomycin and chloromphenicol (83.3%) followed by ciprofloxacin (66.6%) followed by erythromycin and tetracycline (50.0%), while the isolated strains were completely resistant to cephalothin. All L. monocytogenes strains were virulent where all of them were positive to CAMP test and Anton’s test. The PCR results for six studied strains of isolated L. monocytogenes showed that all genes (16S rRNA; inlA; prfA and hlyA) were detected (100.0%) while plcA gene was not detected.
https://bvmj.journals.ekb.eg/article_54506_460eeabbddc1be518e08436e21523b53.pdf
2018-03-01
430
442
10.21608/bvmj.2018.54506
Listeria monocytogenes
chicken
PCR
Virulence genes
Ashraf
Abd El-Tawab
1
Department of Bacteriology, Immunology and Mycology, Faculty of Veterinary Medicine, Benha University, 13736 Moshtoher, Benha, Egypt
AUTHOR
Seham
Homouda
sehamhomouda@yahoo.com
2
Animal Health Research Institute "Tanta branch"
AUTHOR
Shimaa
E. El-Haw
3
Veterinarian
AUTHOR
ORIGINAL_ARTICLE
Detection of adulteration in some traditional processed meat products with equine meat
Sixty random samples of minced meat and sausage each weighting 250 gm (30 of each) were collected from different butchers (15 of each), processing plants (15 of each), in Menofiya and Gharbiya governorates for detection of their adulteration by illegal substitution with cheaper or prohibited meat. Application of PCR technique for detection of adulteration of such products purchased from butcher shops was given, 11 samples of minced meat (73.33%) and 11 samples of sausage (73.33%) were not adulterated, respectively. while 4 samples (26.67%) of minced meat were adulterated (3 samples 20% adulterated by intermixed cattle and equine meat and 1 sample 6.67% adulterated by pure equine meat) and 4 samples (26.67%) of sausage were adulterated (1 sample samples 6.67% adulterated by intermixed cattle and equine meat and 3 samples 20% adulterated by pure equine meat), respectively. Concerning minced meat and sausage samples purchased from processing plants was given, 15 samples (100%) and 13 samples (86.67%) were not adulterated, respectively, (0%) of minced meat were adulterated and 2 samples (13.33%) of sausage were adulterated (1 sample samples 6.67% adulterated by intermixed cattle and pig meat and 1 samples 6.67% adulterated by intermixed cattle and equine meat), respectively. Finally, the obtained data in the current study, PCR technique was the most rapid and accurate method for detection and identification of the meat related to animal species as compared with Precipitation test.
https://bvmj.journals.ekb.eg/article_54507_63ae3a1916111ddbe52bb7074f792c92.pdf
2018-03-01
443
452
10.21608/bvmj.2018.54507
Adulteration
Multiplex PCR
Minced meat
sausage
equine meat
Faten
Hassanin
1
Food Hygiene Dep., Fac. Vet. Med. Benha Univ.,
AUTHOR
Reham
Amin
rehamnour2007@yahoo.com
2
Food Hygiene Dep., Face. Vet. Med. Benha Univ
AUTHOR
Nahla
Abou-Elroos
nahlaabou-elroos@yahoo.com
3
Health Research Institute - “Shebin El-Kom” branch
AUTHOR
Sameh
Helmy
4
3Animal Health Research Institute - “Shebin El-Kom” branch.
AUTHOR
ORIGINAL_ARTICLE
Epidemiological study on Infectious bursal disease in broiler chicken farms in some Governorates in Delta Egypt
This study was conducted to estimate the prevalence of IBD in broiler farms from different localities in different Governorates; AlQalubia, AlGharbia, AlMenofia and ALBehera and also to make a further characterization of the obtained strains through nucleotide sequencing then evaluate the pathogenicity of the strain in SPF ECE and commercial broiler chicks. Fifteen out of a total of 32 (46.87%) bursal samples showed 620 bp amplicon size of HVR of VP2 when tested with RT-PCR. The nucleotide sequencing classified two strains as vvIBDV and one was similar to classical attenuated vaccines. The vvIBDV strain resulted in 100 % mortality of inoculated embryos with moderate to severe congestion of the body, cranial hemorrhage, congested and greenish liver with necrotic foci. The CAMs showed thickening with petechial hemorrhage. The experimental infection with (IBDV_ EGY 2018/N23) isolate was assessed in vaccinated groups with hot, intermediate and hot + intermediate vaccines and unvaccinated 25 days old Cobb broiler chicks; the c+ve group showed a significant difference in RWBF and cumulative bursal lesion in comparison with c–ve group. It recorded higher MSI than c–ve. All vaccinated groups showed a significant increase in Abs titer when compared with non-vaccinated one and the hot vac. Group recorded the highest titer. They also showed a non-significant difference in RWBF and cumulative bursal lesion score in comparison to c-ve one at ten dPI and lower MSI than c+ve but the hot + intermediate vac. group recorded the lowest lesion score and MSI. This study concluded the reemergence and circulation of vvIBDV despite the intensive vaccination strategies.
https://bvmj.journals.ekb.eg/article_101737_c7ed022f26446303a2fea5da0d7be810.pdf
2018-03-01
453
466
10.21608/bvmj.2018.101737
IBDV
RT/PCR
HVR VP2
Bursa of Fabricius
pathogenicity
Mohammed A.
El-Shorbagy
1
Avian and Rabbit Diseases Dept., Fac. Vet. Med. Benha Univ.
AUTHOR
Ahmed E.
Saad
2
Avian and Rabbit Diseases Dept., Fac. Vet. Med. Benha Univ.
AUTHOR
Kamel A.
Zyan
3
Avian and Rabbit Diseases Dept., Fac. Vet. Med. Benha Univ.
AUTHOR
Norhan W.
Khalil
4
Avian and Rabbit Diseases Dept., Fac. Vet. Med. Benha Univ.
AUTHOR
ORIGINAL_ARTICLE
Comparative potentiality study of three different vero cell culture systems for production of PPR Vaccine
Peste des petits ruminants (PPR) is considered one of the most dangerous viral diseases of small ruminants in Africa and Asian countries. The disease control is based on vaccination of susceptible animals with an attenuated PPR virus strain (Nigeria 75/1) propagated in monolayers of Vero cells using stationery and roller flasks. This study for constructing alternative method for production of PPR vaccinal strain through propagation of PPR virus (75/l) on Vero cell culture supported on cytodex-three microcarrier beads using spinner stirring flasks and compare the recorded results with that obtained from stationary flasks (175cm2) and Roller Bottles. All cultures were propagated under the same conditions (media, pH, time of incubation) and infected with the same multiplicity of infection by PPR vaccinal virus. Samples were obtained daily for successive five days from all cultures. The PPR virus titers were 12 log10 TCID50, 5 log10 TCID50 and 6 log10 TCID50 on microcarriers culture, stationary system and roller system respectively after six days post infection. These results provide further insights to apply microcarriers cell culture technology in production of PPR vaccine.
https://bvmj.journals.ekb.eg/article_123434_3ac382d8a4c6c126f6b58aac9db3b276.pdf
2018-03-01
466
473
10.21608/bvmj.2018.123434
PPR
cytodex-3 microcarriers
spinner flasks
row bottles
roller bottles
Gabr
El-Bagoury
gaber.bagoury@yahoo.com
1
Department of Virology, Faculty of Veterinary Medicine, Benha University
AUTHOR
Ehab
EL-Nahas
2
Department of Virology, Faculty of Veterinary Medicine, Benha University
AUTHOR
Lila
Abdelazeem
3
Veterinary serum and vaccine Research institute, Abbasia, cairo.
AUTHOR
Azza
Mohamed
4
Veterinary serum and vaccine Research institute, Abbasia, cairo.
AUTHOR
ORIGINAL_ARTICLE
Optimizing culture conditions for increasing production of vero cell
The key to achieving maximum yields from microcarrier cultures is by using the optimization factors as replenishment of vero cell culture media during growth, the inoculation density on the proportion of microcarriers bearing cells, stirring speed on the growth of vero cell on cytodex 3, culture volume and headspace on cell yield from closed microcarrier system, various culture media, various types of the serum, control of PH, microcarrier concentration and modified initial culture procedure. The result reveled that the higher cell yield obtained after replenishment of culture media every three day, the optimum concentration of microcarrier is to be 3mg cytodex/ml, the optimum inoculation density 15x106 cells/mg cytodex microcarrier is required, the higher cell yield obtained by using (medium 199) containing high amount of amino acid at low density of the cell higher than Dulbecco’s modification of Eagles’s medium (DME) in higher density of the cell, fetal calf serum gave higher cell yield in the first three days in the culture, 60 rpm was the optimum stirring speed to get higher cell yield, addition of 10 – 25 mμ Hepes helped in maintaining the PH around 7.3-7.4 this gave higher cell yield, reduced the head space not more than half full of the culture give a higher result and the modified in initial culture procedure during the growth culture give higher results. The obtained result maximizing the yield of the vero cell production on acytodex-3 microcarrier culture system.
https://bvmj.journals.ekb.eg/article_123444_12db61f5cdd6ea0d45476dbc8b709b1a.pdf
2018-03-01
474
482
10.21608/bvmj.2018.123444
cytodex-3 microcarriers
Initial culture
Stirring speed
HEPES solutions
Serum supplements
Gabr
El-Bagoury
gaber.bagoury@yahoo.com
1
Department of Virology, Faculty of Veterinary Medicine, Benha University
AUTHOR
Ehab
EL-Nahas
2
Department of Virology, Faculty of Veterinary Medicine, Benha University
AUTHOR
Lila
Abdelazeem
3
Veterinary Serum and Vaccine Research Institute, Abbasia, cairo.
AUTHOR
Azza
Mohamed
4
Veterinary Serum and Vaccine Research Institute, Abbasia, cairo.
AUTHOR