Benha University; Faculty of Veterinary Medicine
Benha Veterinary Medical Journal
1110-6581
2974-4806
39
1
2020
09
01
DNA polymorphism of FSHR gene and its association with infertility traits in Egyptian buffaloes
1
4
EN
Sherif
Ramadan
0000-0001-5151-5520
Department of Animal Wealth Development, Faculty of Veterinary Medicine, Benha University, Egypt
sherif.ramadan@fvtm.bu.edu.eg
Abdel-Aziz
Saker
Department of biotechnology, Animal production Research institute, Agriculture Research Center, Cairo, Egypt
abdosakr521@gmail.com
Basant
Shafik
Department of Animal Wealth Development, Faculty of Veterinary Medicine, Benha University, Egypt
basant.shafeek@gmail.com
10.21608/bvmj.2020.34139.1221
The objectives of this study were to detect genetic polymorphism in exon 10 of Follicle Stimulating Hormone Receptor gene (FSHR) by using RFLP and direct sequence methods, also to investigate their possible association with infertility traits in Egyptian buffaloes. A total number of 50 female Egyptian buffaloes at Mahallet Mousa experimental farms in Kafr El-sheikh province were investigated. Animals were divided into three groups: normal fertile (20 animals), infertile due to anestrum (15 animals) and infertile due to repeat breeding (15 animals). Genomic DNA was extracted from blood samples, then PCR was performed with annealing temperatures of 57ºC to amplify 231 bp of FSHR exon 10 gene. The amplicons were digested with Hin1II (NlaIII) restriction enzyme. The results showed that all the investigated buffaloes were monomorphic and genotyped as A/A genotype. Moreover, the monomorphism of buffaloes FSHR exon 10 gene was confirmed by sequencing three samples from each buffaloes’ group. It seems that FSHR gene are highly conserved in the investigated buffalo’s population.
Egyptian buffaloe,fertility,FSHR,Polymorphism,RFLP
https://bvmj.journals.ekb.eg/article_116439.html
https://bvmj.journals.ekb.eg/article_116439_f2c35cc95520698d9790639163fc9a5a.pdf
Benha University; Faculty of Veterinary Medicine
Benha Veterinary Medical Journal
1110-6581
2974-4806
39
1
2020
09
01
The occurrence of pathogenic E. Coli in some types of soft cheeses in the local market
5
10
EN
MOHAMMED
MAHMOUD
WEHABA
government
genome2000@yahoo.com
10.21608/bvmj.2020.37011.1230
The purposes of this practical research to detect the incidence of pathogenic E. coli in kariesh, domiatti and tallaga cheeses. Confirmative serological identifications of the isolated strains of pathogenic E. coli were performed. One hundred and fifty random samples of kariesh, domiatti and tallaga cheeses (50 each) were collected from different shops and street vendors in El Gharbia territories. Samples were bacteriologically examined for the presence of E. coli. The incidence of E. coli in the analyzed samples was 54%, 42%, and 32%, respectively. Serotyping of isolated pathogenic serovars of E. coli were: O119, O111, O86 & O128, and O124 in domiatti cheese. Genotypic identification of E. coli virulence genes (Stx1, eaeA, fimH) were recorded at percentage of Zero %, 28.5% and 100 % respectively. In kariesh cheese. the detected E. coli in the analyzed samples surely able to causes public health disease outbreaks to the consumers and stipulated the local hygienists to insures maximum health’s stipulations.<br /> Therefore this work was planned to covers the followings points:1-Isolations, biochemical and serological identifications of pathological Escherichia coli from kariesh, tallage, and domiatti types of cheeses in El _Gharbia governorate. 2-Molecular identifications of isolated bacteria (bacterium coli) using the PCR technique.
E. coli,selective isolation,in vitro sensitivity,Virulence genes,Soft cheese
https://bvmj.journals.ekb.eg/article_116440.html
https://bvmj.journals.ekb.eg/article_116440_e97dcd8822038c593dbbb3eb5918e3bf.pdf
Benha University; Faculty of Veterinary Medicine
Benha Veterinary Medical Journal
1110-6581
2974-4806
39
1
2020
09
01
Bacteriological profile of some raw chicken meat cuts in Ismailia city, Egypt
11
15
EN
Sayed
Abo elela
Afify
Veterinarian supervisor
ssmm1806@gmail.com
Fahim
Aziz eldin
Shaltout
https://orcid.org/my
Professor of Meat Hygiene,
Department of Food Control,
Faculty of Veterinary Medicine, Moshtohor,
Benha University, Egypt.
Member of the Egyptian Organization for Standerization and Quality : Meat Hygiene Committee.
Member
fahim.shaltout@fvtm.bu.edu.eg
Islam
Zakaria
Mohammed
Chief Researcher, Bacteriology Dept.
Animal Health Research Institute, ARC
islamelshereef@yahoo.com
10.21608/bvmj.2020.37176.1232
One hundred random samples of raw chicken meat cuts represented by breast and thigh (50 of each) were collected from an automatic poultry slaughtering plant in Ismailia city, Egypt. The samples were examined to investigate their bacteriological aspect, where the mean values of aerobic plate count and Enterobacteriacae were 5.9x105 and 5.1x104 CFU/g for breast samples and 7.1x105 and 6.1x104 CFU/g while for thigh samples as well as the incidence of Enterobacteriacae contaminated the breast and thigh samples were 42 and 54%, respectively. Moreover, S. aureus, Salmonellae, E. coli, Enterobacter spp., Proteus spp., Shigella spp. and C. perfringens were detected in 10, 14, 12, 4, 2, 2 and 16% of the examined breast samples, respectively. Referring to thigh samples, S. aureus, Salmonellae, E. coli, Klebsiella spp., Enterobacter spp., Proteus spp., and C. perfringens were detected in 4, 8, 18, 4, 2, 2 and 10%, respectively. The obtained results indicated that the raw chicken meat cuts may harbor many food poisoning bacteria from different sources, which strongly recommends following strict hygienic measures through slaughtering, handling and cooking processes.
Chicken meat,Breast,thigh,Bacteriological aspect
https://bvmj.journals.ekb.eg/article_116441.html
https://bvmj.journals.ekb.eg/article_116441_403b2550616a1f901c7453f4eaa82748.pdf
Benha University; Faculty of Veterinary Medicine
Benha Veterinary Medical Journal
1110-6581
2974-4806
39
1
2020
09
01
Lycopene mitigates experimental colitis in rats by inhibiting oxidative stress-mediated inflammation and apoptosis
16
21
EN
Hend
Hashem
Biochemistry, veterinary medicine,benha university
hendhashem54@gmail.com
Samy
Ali
Hussein
Department of Biochemistry, Faculty of Vet. Med., Benha University, Egypt.
samyaziza@yahoo.com
Yakout
El senosi
0000-0003-0387-6019
Biochemistry, veterinary medicine, benha university
yakout2222@yahoo.co.uk
10.21608/bvmj.2020.37614.1236
Objective: The potential protective and beneficial effects of Lycopene (LYC) in a rat model of acetic acid-induced ulcerative colitis (UC) were evaluated. Methods: Forty male albino rats were divided into five equal groups. Group I: (Control normal) rats received no drugs. Group II: (Early ulcerative colitis): rats received 2 ml (3% v/v) glacial acetic acid intracolonially at 21 th day of experiment and sacrificed 3 days later. Group III:(Early ulcerative colitis + Lycopene protected) rats received Lycopene (10 mg/kg body weight/day, orally) for 21 days prior to acetic acid administration. Group IV: (Late ulcerative colitis) rats received acetic acid similar to group II for 3 successive days and sacrificed after 21 days. Group V: (Late ulcerative colitis+ Lycopene treated) rats first administered with acetic acid then after 3 days Lycopene was administered for 21 days. Results: A significant increase in L-Malondialdehyde (L-MDA) with marked decrease in reduced glutathione (GSH) level and Catalase (CAT) activity were observed in colon tissue of UC-induced rats. Additionally, a significant up-regulation of nuclear factor kappa B (NF-κB), caspase-3 and Transforming growth factor-β1 (TGF-β1) and down-regulation of Interleukin -10 (IL-10) and Beta cell lymphoma-2 (Bcl-2) gene expression levels of were observed in colon of UC induced rats. However, a significant depletion of colon tissue L-MDA and down-regulation of NF-κB, caspase-3 and TGF-β1 in addition to marked increase in GSH concentration and CAT activity and up-regulation of IL-10 and Bcl-2 were observed after LYC treatment. Conclusions: Lycopene has powerful antioxidants, anti-inflammatory and anti-apoptotic effects against ulcerative colitis.
lycopene,colitis,Oxidative Stress,Anti-apoptotic and Inflammatory cytokine
https://bvmj.journals.ekb.eg/article_116442.html
https://bvmj.journals.ekb.eg/article_116442_e936f7494a47904ad8f25299d68f546c.pdf
Benha University; Faculty of Veterinary Medicine
Benha Veterinary Medical Journal
1110-6581
2974-4806
39
1
2020
09
01
Effect Of Some Feed additives (Yeast, Fenugreek seeds and Chamomile flowers) On Some Behavioral patterns and Productive Performance In Pigeons (Columbia livia domestica).
22
27
EN
Eman
Hefnawy
El-ghamry
Animal hygeine and management department, veterinary medicine,benha University, Egypt
emanosamao2016@gmail.com
Mohamed
morsy
karosah
veterinary hygeine and management department, veterinary medicine,benha univerisity,egypt
mohamed.karosah@fvtm.bu.edu.eg
gaffar
mahmoud
el-gendi
animal production departement, faculty of agriculture, benha univerisity, egypt
gaffarelgendy@gmail.com
essam
ali
ahmed
0000-0002-9103-3101
veterinary hygeine and management departement, veterinary medicine, benha univerisity, egypt
essam.mahmoud@fvtm.bu.edu.eg
10.21608/bvmj.2020.36464.1226
This work was carried out at the research center belonging to faculty of agriculture, Benha university through the period from April to the end of September2019 to study the effect of some feed additives (Yeast, Fenugreek seeds and Chamomile flowers) on some behaviors and productive performance of parent pigeons using 16 pairs of hybrid pigeons (Carrier x local Egyptian baladi) with age of 6 months, they were distributed according to their mating system (sex ratio 1:1). Pigeons were divided into 4 treatments each of them composed of 4 pairs and were reared under the same managerial conditions. Obtained results revealed that pigeons fed on Yeast had high frequency of feeding, drinking and sitting on egg also, it increased length of incubation period while, it decreased hatchability %. Fenugreek seeds had high frequency of preening and sexual sitting on egg but, it decreased length of incubation period. Both Fenugreek and chamomile flowers improved hatchability %. From this work, it can be concluded that addition of some feed additives (Yeast, Fenugreek seeds and Chamomile) had the ability to improve behaviors, reproductive and productive performance of adult pigeons.
Feed additives,Pigeons,Behavior,reproductive,productive performance
https://bvmj.journals.ekb.eg/article_116443.html
https://bvmj.journals.ekb.eg/article_116443_261d6813587750835e06bc2018026f56.pdf
Benha University; Faculty of Veterinary Medicine
Benha Veterinary Medical Journal
1110-6581
2974-4806
39
1
2020
09
01
Genetic and Hormonal Difference between High Growth Rate Breed (Cobb Broiler chicken) and Low Growth Rate Breed (Native Fayoumi chicken)
28
33
EN
Reham
Ibrahim
Antar
0000_0003_2053_3982
Physiology,veterinary medicine,kafershoker
vetrehamibrahim@gmail.com
saad
mohamed
shosha
0000-0001-6446-9395
pysiology depatment, faculty of veteriary medicine, benha university
saad.shosha@fvtm.bu.edu.eg
mohamed
elsayed
elazab
physiology department, faculty of vetrinary
medicine, benha university
mohamedalazab@yahoo.com
Randa
saad
esmail
Faculty of Veterinary Medicine, Benha University
randaesmail@yahoo.com
10.21608/bvmj.2020.36634.1229
Abstract<br /> The present study aimed at investigating the genetic and hormonal differences between a low growth rate chicken breed (a native breed: Fayoumi chicken) and a high growth rate chicken breed (Cobb broiler chicken). A total number of 100 one-day-old Fayoumi chicks and 100 one-day-old Cobb chicks were used. Chicks of each breed were allocated into three equal replicates. The growth parameters (body weight, BWG and FCR), genetic expression of some growth related genes (GH, IGF1, ghrelin and Myostatin gene) and plasma level of some growth related hormones (Insulin, T3, T4 and corticosterone) were recorded. The obtained results revealed that, Cobb chicken had significantly higher body weight, BWG and feed intake than Fayoumi chicken but the FCR of Fayoumi chicken was higher than that of broiler chicken. Cobb recorded significantly lower plasma level of corticosterone, T3 and T4 than Fayoumi chicken while Insulin is higher in cobb chicken. Cobb recorded highly significant values of GH, IGF1 and ghrelin Gene expression while myostatin gene expression was higher in Fayoumi chicken than in Cobb From the obtained results could be concluded that, the observed high growth rate in cobb chicken may be attributed the difference gene expression related growth as well as hormonal difference
Key words:- Fayoumi,Broilers,Growth,Gene expression,corticosterone
https://bvmj.journals.ekb.eg/article_116444.html
https://bvmj.journals.ekb.eg/article_116444_03a39eba38b742ad8608fa9263886350.pdf
Benha University; Faculty of Veterinary Medicine
Benha Veterinary Medical Journal
1110-6581
2974-4806
39
1
2020
09
01
Effect of dietary nucleotide supplementation on broiler performance and economic efficiency
34
39
EN
reda
hamed
zahran
0000-0003-2301-8310
nutrition &amp;clinical nutrition ,veterinary medicine,banha university
reda.hamed@fvtm.bu.edu.eg
Nasser
elsayed
Khader
0000-0003-4112-1637
Nutrition and clinical Nutrition, Veterinary medicine, Banha University, Cairo, Egypt
nasser.khader@fvtm.bu.edu.eg
tahia
elsayed
ahmed
nutrition and clinical nutrition, veterinary medicine, banha university, alzaqaziq, Egypt
tahiah.ismail01@fvtm.bu.edu.eg
10.21608/bvmj.2020.37547.1234
A B S T R A C T <br /> <br /> A total number of two hundred forty-one-day-old broiler chicks (Ross 308) were randomly distributed into four different treatment groups (A, B, C and D), of three replicates (20 chicks / replicate). The chicks in control group (A) were fed negative control diet, group B were fed diet containing (Nucleoforce®) (0.025%) in first 10 days of age, group B were fed diet containing (Nucleoforce®) (0.025%) in first 25 days of age. While group D was fed diet containing Nucleoforce® (0.025%) from zero day till slaughtering. This experiment was designed to investigate the effect of nucleotide as an immunostimulant for broiler chicks in terms of chick performance and economic efficiency. Results showed that, groups fed diet supplemented with Nucleoforce recorded improvements in live body weight, body weight gain and relative growth rate with no significant difference in feed intake and feed conversion index than the control group. Economic efficiency showed non-significant difference between control group and other groups but group (D) recorded high value 0.94 more than control group (A) 0.84. It was concluded that, inclusion of nucleotide 0.025% in broiler diets had a positive role in improvement of final body weight, body weight gain, feed conversion ratio and economic efficiency.
broiler chicks,Nucleotide feeding,Chick performance and Economic efficiency
https://bvmj.journals.ekb.eg/article_116445.html
https://bvmj.journals.ekb.eg/article_116445_8bfb3009aea45990ac28b6f209b35441.pdf
Benha University; Faculty of Veterinary Medicine
Benha Veterinary Medical Journal
1110-6581
2974-4806
39
1
2020
09
01
Thymoquinone suppressed Cyclosporine A- induced Nephrotoxicity in rats via antioxidant activation and inhibition of inflammatory and apoptotic signaling pathway
40
46
EN
Eman
Ahmed M.
Sarhan
Biochemistry department, Faculty of veterinary medicine, Beha University, Egypt.
eman.ahmed20@fvtm.bu.edu.eg
Samy
Ali
Hussein
Department of Biochemistry, Faculty of Vet. Med., Benha University, Egypt.
samyaziza@yahoo.com
Yakout
Elsenosi
0000-0003-0387-6019
Department of Biochemistry, Faculty of vtr. Med., Benha University, Egypt
yakout2222@yahoo.co.uk
Tahia
Elsayed Ahmed
Esmael
nutrition and clinical nutrition, veterinary medicine, banha university, alzaqaziq, Egypt
tahiah.ismail01@fvtm.bu.edu.eg
Aziza
Amin
Pathology department, Faculty of Vet. Med., Benha University
aziza.amin159@gmail.com
10.21608/bvmj.2020.33389.1217
Cyclosporine A (CsA) is a strong immunosuppressive drug, but its use is frequently accompanied by severe renal toxicity. The potential renoprotective effect of Thymoquinone (TQ) against CsA-induced nephrotoxicity in rats was assessed. Thirty adult white male albino rats were divided into three equal groups. Group I: (Normal control), received no drugs, Group II: (CsA treated), rats received oral dose of Cyclosporine A (25 mg/kg b.wt/day) for 21 days. Group III: (TQ protected + CsA), received Thymoquinone (10 mg/kg b.wt/day) orally 7 days before and during 21 days of CsA treatment. The obtained results showed a significant increase in the concentration of urea and creatinine in serum and L-MDA level in kidney tissue with marked decrease in renal catalase activity and GSH concentration in CsA treated rats. Moreover, a significant down-regulation in Bcl-2 and up-regulation of NF-κB, PAI-1, Caspase-3 and p53 gene expressions levels were observed in kidney tissues of CsA treated rats. Also, various histopathological alterations were detected in kidneys of CsA treated rats. Meanwhile, TQ potentially improved renal function and oxidative alterations related to CsA near its normal ranges. Interestingly, histopathological findings supported that TQ markedly attenuates harmful effects that CsA induced and protected kidney. Our research could conclude that, TQ has an ameliorating role as potent antioxidant, anti-inflammatory and anti-apoptotic agent via inhibition of inflammatory (NF-κB, PAI-1) and apoptotic (Caspase-3, p53) signaling pathway in modulation of CsA-induced nephrotoxicity.
Cyclosporine A,Thymoquinone,apoptosis,Inflammatory cytokines,histopathology
https://bvmj.journals.ekb.eg/article_116475.html
https://bvmj.journals.ekb.eg/article_116475_493e0eb32503ab4e245b12554d81b2ef.pdf
Benha University; Faculty of Veterinary Medicine
Benha Veterinary Medical Journal
1110-6581
2974-4806
39
1
2020
09
01
Prevalence of Aeromonas spp and their virulence factors isolated from frozen chicken meat products
47
51
EN
Suzan
Hassan
Sheir
Animal Health research institute
alrehab2010exp@gmail.com
Hemmat
Ibrahim
Department of Food Hygiene, Faculty of Veterinary Medicine, Benha University. Animal Health
drhemmat01@yaoo.com
Mohamed
Ahmed
Hassan
Food Hygiene Department, Faculty of Veterinary Medicine, Banha university
mohamedhsn40@yahoo.com
Nahla
Ahmed
Shawky
Animal Health research institute, Shebin El koom branch
dr.nahlashawky@yahoo.com
10.21608/bvmj.2020.37742.1237
A total of hundred samples of frozen chicken products represented by breast, thigh, nuggets and burger (25 of each) were randomly collected to study the prevalence of Aeromonas spp and their virulence factors in the examined products. the study showed that the mean values of psychrotrophic count were 8.17×103± 1.42×103, 1.95×104± 2.06×104, 3.63×104± 0.89×104 and 7.58×104± 1.16×104, respectively, and the mean values of Aeromonas counts were 9.34×102± 2.01×102, 1.66×103± 0.28×103, 2.90×103± 0.43×103 and 5.25×103± 0.69×103 for examined frozen breast, thigh, nuggets and burger. 12 isolates of A. hydrophila were specific for 16S rRNA gene of which 9 isolates were positive for aerolysin (aerA) and 10 of isolates were positive for haemolysin (ahhl), with incidence of 75% and 83.3%, respectively. The results achieved in the current study showed contamination of chicken products by Aeromonas spp. So in the field of food microbiology it is necessary to give more consideration to Aeromonads because they have the ability of toxins production, survival under low temperatures and growing in a wide spectrum of environments. So, hygienic measures should be adopted to control microbial contamination.
Aeromonas spp,Virulence factors,frozen chicken meat products
https://bvmj.journals.ekb.eg/article_116447.html
https://bvmj.journals.ekb.eg/article_116447_530fce4b7723c025db67ebc61da521c8.pdf
Benha University; Faculty of Veterinary Medicine
Benha Veterinary Medical Journal
1110-6581
2974-4806
39
1
2020
09
01
Ultrasonographic Examination of Abdominal Wall Swellings in Cattle and Buffalo (Bubalus bubalis)
58
62
EN
elham
shaaban
khalf
Surgery,Anesthesiology and Radiology/Fuclty of Veterinaary Medicine Benha University / Benha / Egypt
elhamshaban010@gmail.com
Adel
Mohamed
Al-Akraa
Surgery,Anesthesiology and Radiology/Fuclty of Veterinaary Medicine Benha University / Benha / Egypt
alakraa63@yahoo.com
atef
sayed
abd elgalil
Surgery,Anesthesiology and Radiology/Fuclty of Veterinaary Medicine Benha University / Benha / Egypt
atef.abdelgalil@fvtm.bu.edu.eg
ahmed
hassan
khalil
Surgery,Anesthesiology and Radiology/Fuclty of Veterinaary Medicine Benha University / Benha / Egypt
ahmedvsurg@yahoo.com
abdelhalim
mahmoud
elkassapy
Surgery,Anesthesiology and Radiology/Fuclty of Veterinaary Medicine Benha University / Benha / Egypt
abdelhalim.mahmoud@fvtm.bu.edu.eg
Hussein
s
AlMaghraby
Surgery,Anesthesiology and Radiology/Fuclty of Veterinaary Medicine Benha University / Benha / Egypt
hussein.almaghreby@fvtm.bu.edu.eg
10.21608/bvmj.2020.36528.1225
Abstract <br /> A total number of one hundred and fourteen clinical cases (63 cattle and 51 buffaloes) were included in the current study. These cases were suffering from different varieties of abdominal wall swellings (46 abscesses; 49hernias and 19 hematomas) and they were subjected to ultrasonographic examination. From the aforementioned cases the unripend abscess appeared ultrasonographically as a circumscribed echogenic structure with mild distal acoustic enhancement while the ripened abscess appeared as anechoic to hypoechoic structure representing watery to creamy pus contents. The ripened abscess with chronic fibrotic omphalitis revealed a hyperechogenic band with an evident acoustic shadowing. Recent hematomas appeared as anechoic area (blood content) divided by a thin echogenic septa into small chambers toward its center, while old hematoma revealed anechoic to hypoechoic appearance divided by a thick echogenic septa. Reducible hernias revealed disruption of the abdominal wall continuity with intestinal loops and its characteristic peristaltic movement and anechoic, hypoechoic to echogenic contents while irreducible hernias characterized by an evident anechoic inflammatory exudates; reduced peristaltic movement and thick hernial sac with echogenic fibrous adhesions between the sac and the abdominal wall.In conclusion, ultrasonography provides an accurate, non-invasive and fast diagnostic tool for different abdominal wall swellings in cattle and buffalo.
Abdominal wall,Buffalo,cattle,swelling,Ultrasonography
https://bvmj.journals.ekb.eg/article_116446.html
https://bvmj.journals.ekb.eg/article_116446_856327fa21f126f54d727a22815d14ed.pdf
Benha University; Faculty of Veterinary Medicine
Benha Veterinary Medical Journal
1110-6581
2974-4806
39
1
2020
09
01
phenotypic and genotypic characterization of pasteurella multocida isolated from chickens
63
67
EN
Walaa
Zaghloul
Abdelmalek
Microbiology Benha University Egypt menofia
walaazaghloul18@yahoo.com
10.21608/bvmj.2020.35909.1222
A total of 300 samples from apparently healthy chickens were collected in this study.This samples represented as 100 samples from trachea, 100 samples from lung and 100 samples from heart. They were subjected to morphological and biochemical examination for identification of P.Multocida.An incidence of P. multocida by cultural examination was 21 (7%);- 0% from tracheal swabs, 13% from lung and 8% from heart while an incidence by biochemical examination was 6 (2%) ;- 0% from tracheal swabs - 3% from lung and 3% from heart. All suspected P. multocida colonies were subjected to PCR for identification of P. multocida isolates and proved that six isolates were found to be positive for P.multocida with an incidence of 2% which similar to the results of confirmatory biochemical examination.The drug sensitivity test mentioned that P. multocida was sensitive to ciprofloxacin and norfloxacin.Six suspected isolates of P.multocida identified by cultural examination were tested by polymerase chain reaction method by using specific primers for P.multocida . The stn gene was amplified in four isolates giving 617bp, while the InvA gene was amplified in four isolates giving 284bp.
pasturella multocida,chickens,drug sensitivity test,polymerase chain reaction
https://bvmj.journals.ekb.eg/article_116449.html
https://bvmj.journals.ekb.eg/article_116449_4f9a60b4c726ff734852bd7c15e047ef.pdf
Benha University; Faculty of Veterinary Medicine
Benha Veterinary Medical Journal
1110-6581
2974-4806
39
1
2020
09
01
Evaluating the effects of certain probiotics on mycotic contamination in oriental sausage
68
72
EN
mohamed
ibrahim
el-sharkawy
mea hygiene faculty of vet med benha university
dr_shark.vet2009@yahoo.com
reham
abd elaziz
amin
Food Hygiene &Control Dept Fac. of Vet. Medicine Benha University
rehamnour2007@yahoo.com
rasha
abd elhamid
el sabagh
Food Hygiene &Control Dept Fac. of Vet. Medicine Benha University
rasha.alsabagh@fvtm.bu.edu.eg
nahla
ahmed shawky
abo elroos
Chief researcher of food hygiene animal health research institute
Shebein El-Kom
dr.nahlashawky@yahoo.com
10.21608/bvmj.2020.32803.1215
Probiotics are natural antimicrobial agents. It has great beneficial effects to human. In this study, Probiotics as (Lactobacillus casei, Lactobacillus delbrueckii, Bifidobacteriumlactis) were used in oriental sausage to reduce total mould and yeast counts and Aspergillus flavus count. <br /> Tow oriental sausage groups were used. The 1st group was used to evaluate reduction %of total mould and yeast count. It was subdivided to 4 groups 3 were inoculated with different Probiotic strains, the 4th was control. Results showed that total mould and yeast count (cfu/g) reduced to (4.73x103±2.33x102, 6.97x103± 2.73x102 and8.80x103 ± 2.08x102) using Lactobacillus casei, Lactobacillus delbrueckii and Bifidobacterium lactis after 12days inducing reduction % of (91.66, 87.71 and 84.48) respectively.<br /> The 2nd group was used to evaluate reduction %of Aspergillus flavus count. It was inoculated with Aspergillus Flavus then subdivided to 4 groups 3 were inoculated with different Probiotic strains, the 4th was control. Results showed that Aspergillus Flavus count (cfu/g) reduced to (2.97x104±1.86x103, 5.37x104±1.45x103and 6.34x104± 1.45x103) using Lactobacillus casei, Lactobacillus delbrueckii and Bifidobacterium lactis after 12days inducing reduction % of (92.25, 85.98 and 83.45) respectively. <br /> Moreover it was found that Lactobacillus casei was the most effective Probiotics causing the highest reduction% in total mould count and Aspergillus Flavus count followed by lactobacillus delbrueckii then pidfedobacterium lactis.
probiotics,oriental sausage,Aspergillus flavus,Bio preservatives,lactic acid bacteria
https://bvmj.journals.ekb.eg/article_116450.html
https://bvmj.journals.ekb.eg/article_116450_28c3cf97e230cf99ff63c7734bf5af28.pdf
Benha University; Faculty of Veterinary Medicine
Benha Veterinary Medical Journal
1110-6581
2974-4806
39
1
2020
09
01
E. coli and Salmonella stereotyping in cattle carcasses at abattoir level
73
76
EN
Hemmat
Ibrahim
Department of Food Control, Faculty of Vet. Med., Benha University, Egypt.
hemmatibrahim@yahoo.com
Mohamed
Hassan
Department of Food Control, Faculty of Vet. Med., Benha University, Egypt.
mohamed.hassan@fvtm.bu.edu.eg
Reham
Amin
Department of Food Control, Faculty of Vet. Med., Benha University, Egypt.
rehamnour2007@yahoo.com
Hend
salah
almokadem
department of meat hygiene, faculty of veterinary medicine, benha university.
hendalmokadem254@gmail.com
10.21608/bvmj.2020.36055.1223
Ninety random samples of fresh beef were collected from the cattle carcasses slaughtered at 3 different abattoirs in Menoufia governorate namely A, B and C (30 of each), represented by neck region (chuck) during summer and winter seasons in 2018 (45 samples of each season). The obtained results of serotyping of E. coli revealed that EHEC O26: H11 were the more prevalent than other serogroups 6.67%, 6.67% &13.33% and 6.67%, 13.33% & 6.67% .T The results of the total incidence were 46.67% , 40% & 26.67% and 26.67%, 26.67% & 20 for A, B&C abattoirs during the summer and the winter seasons , respectively. Serotyping of salmonella revealed S.Typhimurium was more prevalent during the summer 13.33%for each abattoir, the total isolation rate 33.33%, 26.67% & 20% for A, B and C abattoirs, respectively. The more prevalent serogoup during the winter was S. Enteritidis 13.33%, 6.67% and 6.67%,while the total isolation rate was 26.67% , 20% and 6.67% for A, B &C abattoirs, respectively .The contamination rate increased in abattoir A than B than C and the prevalence was increased during summer than in winter
E.coli,Salmonella,Serotyping,isolation.seasonal variation
https://bvmj.journals.ekb.eg/article_116451.html
https://bvmj.journals.ekb.eg/article_116451_54b7eece111d1cee654cf34bba485c50.pdf
Benha University; Faculty of Veterinary Medicine
Benha Veterinary Medical Journal
1110-6581
2974-4806
39
1
2020
09
01
The effect of different disinfectants against some food poisoning bacteria at abattoir level
77
79
EN
Hend
salah
almokadem
department of meat hygiene, faculty of veterinary medicine, benha university.
hendalmokadem254@gmail.com
10.21608/bvmj.2020.36059.1224
Ninety random samples of fresh beef were collected from cattle carcasses slaughtered at 3 different abattoirs in Menufia governorate (30 sample of each). The sampling site was represented by neck region (chuck) of each carcass in 2018. The obtained results revealed that the efficacy of five different disinfectants against some food poisoning bacteria including E. coli O26: H11, S. Typhimurium and Klebsiella pneumonia which were isolated and serotyped at abattoir level. In vitro infected samples (3.0×106/ cm2) were examined under effect of different concentrations of disinfectants within contact time 120 minute. Disinfectant (A) at concentration 1% was effective against 100% of E. coli O26: H11 and Klebsiella pneumoniae, while 1.5% concentration was required for elimination of 100% S. Typhimurium, followed by B, C, D and E disinfectants, respectively. On the other D and E disinfectants even in 2% concentration and contact time 120 minutes reduce about 99% of the infection.
disinfectants,Food Poisoning Bacteria,Abattoir contamination
https://bvmj.journals.ekb.eg/article_116452.html
https://bvmj.journals.ekb.eg/article_116452_20dbb23b38c52c1b37185c689e4494a4.pdf
Benha University; Faculty of Veterinary Medicine
Benha Veterinary Medical Journal
1110-6581
2974-4806
39
1
2020
09
01
Natural antifungal agents for food preservation
80
84
EN
Mahmoud
Hammad
Food Quality Control Program (FQCP), Faculty of Veterinary Medicine, Benha University.
bvmj2020@gmail.com
Ekbal
Ibraheem
Food Quality Control Program (FQCP), Faculty of Veterinary Medicine, Benha University.
Hend
Elbarbary
Food Quality Control Program (FQCP), Faculty of Veterinary Medicine, Benha University.
10.21608/bvmj.2020.26306.1191
The increasing societal demand for less processed and more natural food products—while conserving those products’ quality, safety, and shelf-life—has raised the question of chemical preservative replacement. In this context, bacteria and fungi as well as their metabolites are natural alternatives of interest for use in food as bioprotective tools to fight fungal spoilage and to answer consumer demands and legislation.Food spoilage is a major issue for the food industry, leading to food waste, substantial economic losses for manufacturers and consumers, and a negative impact on brand names. Among causes, fungal contamination can be encountered at various stages of the food chain (e.g., post-harvest, during processing or storage. Fungal development leads to food sensory defects varying from visual deterioration to noticeable odor, flavor, or texture changes but can also have negative health impacts via mycotoxin production by some molds. In order to avoid microbial spoilage and thus extend product shelf life, different treatments—including fungicides and chemical preservatives—are used. In parallel, public authorities encourage the food industry to limit the use of these chemical compounds and develop natural methods for food preservation.
Natural antifungal agents,Food preservation,Food spoilage
https://bvmj.journals.ekb.eg/article_116453.html
https://bvmj.journals.ekb.eg/article_116453_845e2bf5b934785a5ec313290b7a1275.pdf
Benha University; Faculty of Veterinary Medicine
Benha Veterinary Medical Journal
1110-6581
2974-4806
39
1
2020
09
01
hepatoprotective effect of ethanolic extract of Origanum vulgare against doxycycline toxicity.
85
90
EN
dawlat
elkhalal
histology departement -national organization of drug control and research
dodyelkhalal83@gmail.com
Ehab
Alzaghaby
histology department -veterinary medicine -Banha university
ehab.alzaghaby@fvtm.bu.edu.eg
hosam
attia
histology department veterinary medicine banha university
hhh-hosam@hotmail.com
sherin
hamad
histopathology department- national organization of drug control and research
manaa_82@yahoo.com
10.21608/bvmj.2020.21307.1160
The current study evaluates the ameliorative action of Origanumvulgareethanolic extract against the hepatotoxic effects of experimental doxycycline overdose in rats.<br /> Forty male Sprague-Dawley rats (160–200 gm) were divided into four groups: the control group, administered distilled water (10 ml/kg b.w.); group (2) administered doxycycline (39.2 ml/kg b.w.) in distilled water; group (3) administered O. Vulgare ethanolic extract (20 mg/kg b.w.) +doxycycline (39.2 ml/kg b.w.) in distilled water and group (4) administered O. Vulgare ethanolic extract (60 mg/kg b.w.) + doxycycline (39.2 ml/kg b.w.) in distilled water. All rats were administrated once per day for 4weeks.<br /> Doxycycline administrated rats showed alteration in histological structure in the liver as vacuolization, necrosis, inflammation, and sinusoidal dilatations. Also increased Kupffer cells population and congestion and dilatation of central vain with fibrin thrombi filling their lumen were shown.Ultra structurally destructed cell membrane of hepatocyte, Pyknotic and karyolitic nuclei, losses in some cytoplasmic organelles, swollen mitochondria, fragmentation of rER and discarded lysosomes were observed. All of those improved by the ethanolic extract of Origanum vulgare.
rat,Doxycycline,oregano,Liver,histology
https://bvmj.journals.ekb.eg/article_116454.html
https://bvmj.journals.ekb.eg/article_116454_41508a7ef9afb60b2621ac7b4d58ca47.pdf
Benha University; Faculty of Veterinary Medicine
Benha Veterinary Medical Journal
1110-6581
2974-4806
39
1
2020
09
01
Effect of Different Cooking Methods on Bacteriological Quality of Meat
91
94
EN
hany
ahmed
hogoo
faculty
hanyhogoo@gmail.com
10.21608/bvmj.2020.24980.1177
A grand total of 100 random samples of fresh meat collected from different supermarkets and shops from Menoufia governorate. and then divided into four groups, raw, boiled, fried, and roasted evaluated bacteriogically ( total APC, coliform count, St.aureus count, Staphylococcal count) .The bacteriological examination revealed that the mean values of APC of the raw ,boiled ,fried and roasted meat samples were 2.28x107±6.16x106a. ,1.19x103-±2.92x102b. , 1.50x104.± 4.24x103b , 2.70x103- ±1.55x103b cfu/g. .The mean values of coliform count(cfu/g) of raw ,boiled ,fried ,roasted ,meat samples were 1.35x105 ±5.06x104a , 9.2x10±3.00x10b , 2.78x102±8.90x10b ,4.41x102 ±2.08x102b ...The mean value of Staphylococci count(cfu/g) of raw,boiled, fried, roasted meat samples were 7.75x103±2.42x103. 3.48x10±0.11x102b, 0.60x102 ±0.17x102b , 0.92x102±0.27x102b . The mean values of the S. aureus count of the raw ,boiled ,fried ,roasted meat samples were5.12x103± 1.46x103a .,2.62x10 ±0.80x10b , 0.33x102±0.10x102b , 068x102±0.20x102b.Methods of heat treatment of meat have acceptable killing effect on reduction of the bacterial load of raw samples.The boiling method is the first method for reduction of bacterial load of raw meat followed by frying method then roasting method.
boiled meat,fried meat,roasted meat,APC,S. aureus
https://bvmj.journals.ekb.eg/article_116455.html
https://bvmj.journals.ekb.eg/article_116455_5c9c6a567bc1c9076c5efe767d7e46e4.pdf
Benha University; Faculty of Veterinary Medicine
Benha Veterinary Medical Journal
1110-6581
2974-4806
39
1
2020
09
01
Curcumin and Hesperidin alleviates oxidative stress and hormonal alterations in a rat model of cyclophosphamide-induced premature ovarian failure
95
100
EN
khaled
helmy
elnahas
Department of Biochemistry,Faculty of Veterinary Medicine,Benha University,egypt
khelmy762@gmail.com
Samy
Ali
Aziza
0000-0003-0387-6019
Biochemistry Department, Faculty of Veterinary Medicine, Benha University, Egypt.
samy.aziza@fvtm.bu.edu.eg
Yakout
Abdelfattah
El-Senosi
Biochemistry Department, Faculty of Veterinary Medicine, Benha University, Egypt.
yakout.alsnoussi@fvtm.bu.edu.eg
Mohsen
Abdelhafez
Aggag
Theriogenology Department, Faculty of Veterinary Medicine, Benha University, Egypt.
mohsan.aggag@fvtm.bu.edu.eg
10.21608/bvmj.2020.28289.1200
Premature ovarian failure (POF) is a cessation of ovarian function in women under 40 years of age. Ovarian atrophy leads to a reduced follicle reserve, which leads to menstrual irregularities, ovarian dysfunction, and infertility. The beneficial effects of curcumin (CRC) and hesperidin (HSP) on Cyclophosphamide –induced Premature ovarian failure (POF) in a rat model were studied. POF was induced by intraperitoneal (i.p) injection of cyclophosphamide (200 mg/kg b.wt) at day one, and then (8 mg/kg b.wt/day) for the following 14 days. Two weeks after POF induction, treatment with CRC (100 mg/kg b.wt/day, i.p) and HSP (80 mg/kg b.wt/day, i.p) were started and continued for 14 days. Ninety female rats were divided into six equal groups. Group 1( Control normal group) rats received no drugs, Group 2 (POF-induced group), Group 3 (POF + CRC treated), Group 4 ( POF + HSP treated), Group5 ( POF + CRC+HSP treated) and Group 6 (Normal+CRC+HSP treated). Serum follicle-stimulating hormone (FSH) and ovarian tissues Malonedialdehyde (MDA) concentrations were significantly increased while serum estradiol (E2) level, ovarian superoxide dismutase (SOD) activity and reduced glutathione (GSH) concentration were markedly decreased in POF group as compared with control group. However, a significant increase in serum E2 , ovarian tissues SOD activity and GSH level and marked decrease in FSH and MDA concentrations were observed in rats treated with CRC or/and HSP compared with the POF group. Conclusively, curcumin and hesperidin provided an effective treatment for Premature ovarian failure induced by Cyclophosphamide in rats.
premature ovarian failure,cyclophosphamide,curcumin, hesperidin,Oxidative Stress
https://bvmj.journals.ekb.eg/article_116456.html
https://bvmj.journals.ekb.eg/article_116456_46cfdf6f1654ad53d983bc49c7aa5ca8.pdf
Benha University; Faculty of Veterinary Medicine
Benha Veterinary Medical Journal
1110-6581
2974-4806
39
1
2020
09
01
Detection of E. coli O157 and Salmonella species in some raw chicken meat cuts in Ismailia province, Egypt
101
104
EN
Sayed
Aboelela
Afify
Meat hygiene banha university
ssmm1806@gmail.com
Fahim
Shaltout
https://orcid.org/my
Professor of Meat Hygiene,
Department of Food Control,
Faculty of Veterinary Medicine, Moshtohor,
Benha University, Egypt.
Member of the Egyptian Organization for Standerization and Quality : Meat Hygiene Committee.
Member
fahim.shaltout@fvtm.bu.edu.eg
Islam
Zakaria
Mohammed
Chief Researcher, Bacteriology Dept.
Animal Health Research Institute, ARC
islamelshereef@yahoo.com
10.21608/bvmj.2020.38391.1239
One hundred random fresh raw chicken breast and thigh samples (50 of each) were collected from an automatic poultry dressing plant in Ismailia city, Egypt for bacteriological and genetic detection of E. coli O157:H7 and Salmonella species. Out of the examined 100 samples, only 4 (4%) samples were contaminated with E. coli O157:H7, while non-O157 E. coli isolates represented 11% of E. coli isolates which were serotyped as O114:H21, O127:H6, O126 and O26 with incidence of 2, 4, 3, and 2% of the examined samples, respectively. On the other side, Salmonellae were detected in 11 samples (11%) and serologically identified as S. Typhi, S. Typhimurium and S. Enteritidis with the incidence of 1, 3, and 7%, respectively. Further, PCR investigation indicated that fliC gene was detected in all 4 isolates of E. coli O157 with incidence of 100%; while, fimA gene in 5 Salmonella isolates was detected in 4 (80%) of such examined isolates. Accordingly, it is obvious that raw chicken meat cuts were loaded with pathogenic foodborne bacteria exposing consumers to the high risk of food poisoning. Moreover, the results cleared that bacteriological traditional methods for detection of bacteria contaminating foods are labor-intensive and time-consuming but PCR are more rapid and highly sensitive for identification of foodborne pathogens.
Chicken meat,fliC gene,fimA gene,E. coli O157:H7,Salmonellae
https://bvmj.journals.ekb.eg/article_116457.html
https://bvmj.journals.ekb.eg/article_116457_784bed60cac4eefd7483de075b674987.pdf
Benha University; Faculty of Veterinary Medicine
Benha Veterinary Medical Journal
1110-6581
2974-4806
39
1
2020
09
01
Clinico-pathological alterations in Holstein dairy cows with digital dermatitis with therapeutic intervention
105
110
EN
Mourad
Mahmoud
Mahmoud
0000-0002-2650-7745
Department of Animal Medicine, Faculty of Veterinary Medicine, Beni-Suef University, Beni‑Suef 62511, Egypt
morad.mahmoud@vet.bsu.edu.eg
Emad
Abdel-Hamied
Department of Animal Medicine, Faculty of Veterinary Medicine, Beni-Suef University, Beni‑Suef 62511, Egypt
emad.abdelmaksoud@vet.bsu.edu.eg
Emad
Ahmed
Mahdi
Department of Pathology, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef 62511, Egypt
emadmahdi@vet.bsu.edu.eg
Mahmoud
Sayed
Sirag
Department of Pathology, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef 62511, Egypt
mahmoud.sayed@vet.bsu.edu.eg
10.21608/bvmj.2020.39119.1244
This study was performed on a commercial dairy herd of 127 Holstein cows with an outbreak of digital dermatitis (DD). Fifty-four DD-affected cows and 20 clinically healthy cows of the same days in milk (DIM) were selected. A detailed examination of each cow feet was recorded. Gross description and anatomic distribution of DD lesions were recorded. Lesions were biopsied and evaluated histopathologically. Lameness scoring was performed. Milk yield and BCS of the DD-affected and healthy cows were compared. Effectiveness of topical oxytetracycline treatment was assessed. The disease was contagious, a total of 54/127 cows were affected in which 70 lesions were identified. The consistent findings were lameness, reduced mobility and a localized painful dermatitis on hind claws. Most cows were clinically lame (62.9%). In the majority (92.6%) of cows only one hind leg was affected, and the left was more affected than the right one (51.9% vs 40.7%). The lesions most commonly (55.6%) were located on the back of hind legs on heel region. Majority of lesions were M2 (44.3%), large size (74.3%), painful (82.9), pink/red (62.9%), deep (42.9%), and circular (60%). Milk yield and BCS significantly decreased in DD-affected cows as compared to healthy cows. Lesions were responsive to topical oxytetracycline treatment. In conclusion, DD is a major cause of lameness in dairy farms and has a negative impact on milk yield and BCS. High incidence of lesions on the back of hind claws is characteristic for DD. Topical oxytetracycline is effective in DD lesions treatment.
Digital dermatitis,clinical score,Pathology,Oxytetracycline
https://bvmj.journals.ekb.eg/article_116458.html
https://bvmj.journals.ekb.eg/article_116458_a4f17c7088d095b14dc75a00e9032052.pdf
Benha University; Faculty of Veterinary Medicine
Benha Veterinary Medical Journal
1110-6581
2974-4806
39
1
2020
09
01
Insight into summer mortality syndrome in farmed Nile tilapia (Oreochromis niloticus) associated with bacterial infection.
111
118
EN
Amel
M
El Asely
0000-0002-1623-6363
Department of Aquatic animals diseases and Management, Faculty of veterinary medicine, Benha university
amlvet@yahoo.com
Hadeer
Youssuf
Department of Aquatic Animals Diseases and Management, Faculty of Veterinary Medicine, Benha University, Egypt
Eman
Abdel Gawad
Department of Aquatic Animals Diseases and Management, Faculty of Veterinary Medicine, Benha University, Egypt
Hiam
Elabd
Department of Aquatic Animals Diseases and Management, Faculty of Veterinary Medicine, Benha University, Egypt
Aya
Matter
Department of Aquatic Animals Diseases and Management, Faculty of Veterinary Medicine, Benha University, Egypt
aya.mater@fvtm.bu.edu.eg
Adel
Shaheen
Department of Aquatic Animals Diseases and Management, Faculty of Veterinary Medicine, Benha University, Egypt
shaheen_aa@yahoo.com
Amany
Abbass
0000-0001-7061-8496
Department of Aquatic Animals Diseases and Management, Faculty of Veterinary Medicine, Benha University, Egypt
amanyabbass65@gmail.com
10.21608/bvmj.2020.40404.1255
The present study was carried out to determine the possible causes of summer mass mortalities among farmed Nile tilapia, Oreochromis niloticus. Pure bacterial colonies were isolated from moribund O. nitoticus from 13 different fish farms; which suffered from high mortalities ranged from (50-80%), during the period from April to October, 2018. Fish showed external hemorrhagic spots, skin darkening, abdominal distension and exophthalmia. Internally, congestion and enlargement of internal organs with serous or hemorrhagic fluid was the most obvious picture. Based on the phenotypic and biochemical characterization using API20E, the isolated bacteria were identified as (A. veronii, A. hydrophila, A. caviae and A. sobria; Aeromonads), (Ps. Fluorescence; Pseudomonas spp), (E. sakazakii and E. cloacae; Enterobacter spp), (C. freundii; Citrobacter spp), (S. odorifera, S. liquefaciens, and S. marcescens; Serratia spp), (S. lutiensis, S. equine; Streptococcus spp), Lactococcus lactis and Proteus vulgaris; with the most prevalence to Aeromonads. Most isolates were accurate identified by PCR and gene sequencing. Water physicochemical parameters were measured at the farm site; which showed an increase in the level of both pH and ammonia. In order to confirm the pathogenicity of the bacterial isolates, an experimental infection was conducted using different doses. The results revealed that A.veronii (HY2) at dose of 9×108 cells/ml was the most pathogenic , with mortality rate 100 %. This study concludes that A. veronii, C. freundii, P.vulgaris and P. fluorescens are implicated in summer mortality of Nile tilapia, without neglecting the role of water quality in worsening the problem.
. niloticus,Mass moralities,gene sequencing,bacterial infection
https://bvmj.journals.ekb.eg/article_116459.html
https://bvmj.journals.ekb.eg/article_116459_684e333c39e3e993135f818df0495fcc.pdf
Benha University; Faculty of Veterinary Medicine
Benha Veterinary Medical Journal
1110-6581
2974-4806
39
1
2020
09
01
Incidence and Economics of Clinical Mastitis of Holstein Friesian Dairy Cows under Egyptian Condition
119
124
EN
Amira
Mohammed
Abd-El Hamed
0000-0002-2559-4314
Animal wealth development department, Faculty of veterinary medicine,Benha university,Quwesina ,Egypt
meroelfeky1000@gmail.com
Eman
Kamel
Animal Wealth Development Department, Faculty of veterinary medicine,Banha university
eman.ramadan05@yahoo.com
10.21608/bvmj.2020.40775.1259
Mastitis is an endemic disease that is considered to be one of the most frequent and costly dairy disease, so the aim of this study was to investigate different factors that affecting mastitis incidence, and their effect on productivity and profitability of dairy farms. Data used in this study were estimated from 1353 lactation records of Friesian dairy cows within private and governmental farms. The productive, reproductive and economic data for a whole lactation season were recorded for healthy and mastitic cows. Four risk factors for mastitis were included (production sector, calving season, parity and milk production level). Data were classified according to risk factors into two production sectors private and governmental, two calving seasons, summer and winter and Six lactation orders from 1stto 6th, two milk production levels (high and low milk producing cows). Winter calving, older and high producing cows and private sector had the highest mastitis incidence, mastitis reduced the 305MY of Mastitic cows by 33.7 & 20.3Kg during disease period, and 62.7 & 37.7Kg milk was discarded during treatment period for private and governmental sector respectively. Finally economic losses of mastitis per animal was 695.7 EGP yearly.
Key words: Mastitis,Incidence,305My,Economic
https://bvmj.journals.ekb.eg/article_116460.html
https://bvmj.journals.ekb.eg/article_116460_05c095023edd88e8bc87d6cda85021cc.pdf
Benha University; Faculty of Veterinary Medicine
Benha Veterinary Medical Journal
1110-6581
2974-4806
39
1
2020
09
01
Bacteriological quality guides in local and imported beef and their relation to public health
125
129
EN
Faten
Hassanien
Food Hygiene,Vet. Medicine, Benha University
fatensayed1960@gmail.com
fahim
Aziz Eldeen
shaltout
food hygiene department,benha university
fahimshaltout@hotmail.com
marionette
Zaghloul
Fahmey
Food Hygiene Dept., Animal Health Research Institute, Benha branch
marionettezaghloul@yahoo.com
Hassan
Fehr Abdelkader
Elsukkary
Meat Hygiene, Vet. Medicine, Benha University
hassanfehr@gmail.com
10.21608/bvmj.2020.32598.1214
Contamination of meat products are thought to be the most significant key sources of serious diseases, especially foodborne diseases that spreading all over the world. A total of 100 random samples of chilled local and frozen imported beef (50 of each) were collected from local super markets and retail shops from Cairo governorate for evaluation of their quality from bacteriological point of view . Aerobic plate count (cfu/g )in the local samples were between 1.1×106 and 4.4 ×107 with an average of 4.1×107± 0.02×106 while , in frozen imported were ranged between 2.6×107 to 5.3 ×108 with an average of 2.8×107± 0.03 ×107 . Moreover, Coliform count (cfu/g ) in local samples were ranged from 1×103 to 1.2×104 with average of 4.2x103 ± 0.03×103 , while in imported samples were between 6×102 and 11.0×103 with average of 7.1x103 ± 0.02×103 . Eschericia coli were detected in 4% of chilled samples and 2% of frozen samples. Salmonella spp. has been detected in 4% of local samples while all frozen samples were free. From the overall results, we can conclude that both chilled local and frozen imported meat are considered as a significant source of bacteriological public health hazard and need a special control attention.
beef,local,imported,Bacteriological,Evaluation
https://bvmj.journals.ekb.eg/article_116461.html
https://bvmj.journals.ekb.eg/article_116461_dfa939c05ad2aaab4a0a816ec1e640e7.pdf
Benha University; Faculty of Veterinary Medicine
Benha Veterinary Medical Journal
1110-6581
2974-4806
39
1
2020
09
01
Antibacterial effect of pepper and cumin extracts on some pathogens contaminating chicken meat
130
134
EN
suhair
nagy
Shehab ElDin
Veterinarian, Directorate of Veterinary Medicine, Qalubiya governorate, Egypt
thepure2008@gmail.com
Saad
Mahmoud
Saad
Food control Dept., Fac. Vet. Med., Benha University, Egypt
s.mahmoud@fvtm.bu.edu.eg
Hemmat
Moustafa
Ibrahim
Food control Dept., Fac. Vet. Med., Benha University, Egypt
drhemmat01@yahoo.com
Mohamed
Ahmed
Hassan
Food Hygiene Department, Faculty of Veterinary Medicine, Banha university
mohamedhsn40@yahoo.com
10.21608/bvmj.2020.31028.1212
Prevention of food poisoning bacteria is usually achieved by using of some chemical preservatives which have public hazards coming through the improper use of it including presence of chemical residues in food, and acquisition of microbial resistance. Based on such safety concerns, the public need to replace it with more potentially effective, healthier natural alternative preservatives are increased. In the present study, two plant extracts, represented by black pepper and cumin extracts were examined to inhibit the growth of foodborne Staphylococcus aureus, Escherichia coli and Salmonella Typhi strains that were artificially inoculated into minced chicken meat samples. Results revealed that the used pepper and cumin extracts significantly reduced the count of tested strains by 102 CFU/g with reduction percent exceeded 99.0%, which proved that the used herbal extracts were potentially effective with variable efficiency against the tested bacterial strains; so, it can be used as natural alternative preservatives to control food poisoning diseases and preserve food stuff avoiding health hazards of chemically antimicrobial agent applications.
black pepper,cumin,Staphylococcus aureus,E. coli,Salmonella Typhi
https://bvmj.journals.ekb.eg/article_116462.html
https://bvmj.journals.ekb.eg/article_116462_9d521b14beabb3ff94af11f7c515b1ab.pdf
Benha University; Faculty of Veterinary Medicine
Benha Veterinary Medical Journal
1110-6581
2974-4806
39
1
2020
09
01
Rapid detection and isolation of Foot and Mouth Disease Virus in samples from clinically suspected animals in Egypt during 2016-2019.
135
140
EN
Fouad
Saad
El-mayet
0000-0003-3133-3935
Virology department- faculty of Veterinary medicine- Benha University
fouad.saad@fvtm.bu.edu.eg
Saad
Sharawy
0000-0003-3647-2440
Department of Virology- Faculty of Veterinary Medicine- Benha University
saad.sharawy@fvtm.bu.edu.eg
Ayman
Said
El-Habbaa
0000-0003-3514-3573
Department of virology, faculty of veterinary medicine, Benha university
ayman.alhbbak@fvtm.bu.edu.eg
Nehal
Shahen
Animal Health Research Institute, Benha branch, Egypt
nono-yara@hotmail.com
Naglaa
Hagag
0000-0002-2598-3264
Animal Health Research Institute, Benha branch, Egypt
naglaahagagahri@gmail.com
10.21608/bvmj.2020.30110.1208
Foot and Mouth Disease (FMD) is a highly contagious and economically important disease of susceptible cloven-hoofed animals, mostly for cattle, buffalo and pigs. This study was designed to isolate and identify the serotypes of FMD virus from clinically suspected animals in different localities in Egypt that would be useful to detect the current strains present in Egypt to be used in the future vaccination program. Tongue epithelium, vesicular fluid and heart tissue samples were collected from FMDV clinically suspected cases representing four different governorates in Egypt (Qalubia, Sharkia, Gharbia and Behera). We found that 32 from 45 submitted samples showed positive result in real time RT-PCR and about 26 of these positive samples were isolated on BHK-21 cells giving overt cytopathic effect of the virus. The isolated viruses were identified and serotyped using antigen detection ELISA and RT-PCR that confirmed the three different serotypes A, O and SAT2 with different ratio for their prevalence in Egypt. We found that SAT2 was the predominant circulating serotype in the field, followed by serotype O and serotype A. Hence, this work demonstrates the co-occurrence of three different FMDV serotypes in Egypt. Consequently, further molecular analyses are recommended to confirm these findings to determine the molecular epidemiology of the isolates and to update the nature of future vaccine strains for successful preventive strategies.
FMDV,Real time RT-PCR,isolation,BHK-21 cells,Genetic Characterization
https://bvmj.journals.ekb.eg/article_116463.html
https://bvmj.journals.ekb.eg/article_116463_1bda6e8dfefb3987de9379765a43d9a9.pdf
Benha University; Faculty of Veterinary Medicine
Benha Veterinary Medical Journal
1110-6581
2974-4806
39
1
2020
09
01
Chemical quality of some fresh water fish in Egyptian markets
141
145
EN
ahmed
mohammed
mostafa
food hygiene department, Animal Health research Institute, Dokki.
dr_ahmed_gado64@yahoo.com
10.21608/bvmj.2020.39495.1250
Total ninety random meat samples of fresh fish of Nile tilapia, Bagrus bayad and Clarias gariepinus (thirty of each) were collected from different Egyptian fish markets to be examined for their chemical quality indices to determine their fitness for human consumption. The results showed that the pH value was (6.32±0.01, 6.38±0.02 and 6.29±0.02), TVB-N (mg/100) was (14.54± 0.51, 12.71± 0.43 and 16.76± 0.46), TBA (mg malonaldyde/kg) was (0.82± 0.05, 0.57 ± 0.03 and 0.91± 0.06) and TMA (mg/100) was (6.19± 0.23, 5.49± 0.21 and 6.75± 0.19) in the examined samples of Nile tilapia, Bagrus bayad and Clarias gariepinus, respectively. So, it was appeared that the Clarias gariepinus samples were higher in their pH, TVB-N, TMA, TBA values than other examined samples. All examined samples were fit for human consumption except one Clarias gariepinus sample (3.33%) was unfit due to increasing its pH value than the limit of acceptability. This study shows superiority of Bagrus bayad were more than Nile tilapia followed by Clarias gariepinus samples in their chemical quality indices. So, we recommend proper handling of fish from catching till eating to preserve their human consumption fitness.
Bagrus bayad,pH,TVB-N,tba,TMA
https://bvmj.journals.ekb.eg/article_116464.html
https://bvmj.journals.ekb.eg/article_116464_ec851a917726aa1f3a6f313dd7ffa2e5.pdf
Benha University; Faculty of Veterinary Medicine
Benha Veterinary Medical Journal
1110-6581
2974-4806
39
1
2020
09
01
Effect of Calving season and Parity on the Productivity and Profitability of Holstein Friesian Dairy Farms
146
153
EN
Amira
Mohammed
Abd-El Hamed
0000-0002-2559-4314
Animal wealth development department, Faculty of veterinary medicine,Benha university,Quwesina ,Egypt
meroelfeky1000@gmail.com
Eman
Kamel
Animal Wealth Development Department, Faculty of veterinary medicine,Banha university
eman.ramadan05@yahoo.com
Sanad
Atallah
Economics, Marketing and Farm Management, Department of Animal Wealth Development, Faculty of Veterinary Medicine, Alexandria University.
10.21608/bvmj.2020.40558.1256
Abstract<br /> The aim of this study is to evaluate the effect of calving season and parity on the productivity and profitability of private and governmental dairy farms in Egypt. The data of this study were estimated from 1353 lactation records of Friesian dairy cows. The data were classified according to calving season into winter and summer calvers, and according to parity into six lactation orders from 1st to 6th, then the data were analyzed statistically by using the computer programs SPSS/PC+ version 23. Total milk yield (305MY) and net profit (NP) differed significantly between calving season and parity within production sector, private sector for winter calvers and 3rd parity had the highest 305MY and NP (9759.5Kg - 40960.9EGP&10304.3 kg, 44546.1EGP, respectively), while governmental sector for summer calvers and 6th parity had the lowest 305MY and NP (5028.4Kg-12117.1EGP & 4460.1kg,10027.3EGP, respectively). Total cost (TC) differed significantly within parity and sector not season, it was the highest for private sector at 4th parity (34849.1EGp), while 6th parity for governmental sector had the lowest value (27657.5 EGP). Finally we concluded that winter calvers and 3rd parity achieve the highest profitability for dairy farms.
Key words: Calving season,parity,305My,Economic,Friesian
https://bvmj.journals.ekb.eg/article_116465.html
https://bvmj.journals.ekb.eg/article_116465_2593cf1a0f333ab000aa0e0ea5a6c47a.pdf
Benha University; Faculty of Veterinary Medicine
Benha Veterinary Medical Journal
1110-6581
2974-4806
39
1
2020
09
01
Bacteriological Quality of Canned Meat Marketed in Egypt
154
158
EN
Nasser
sayed
Abdel-Atty
Food hygiene and control department, Fac. Vet.Med., Beniusuef university, Egypt
nasser_774@yahoo.com
fathy
ahmed
khalafalla
Food hygiene dept., fac. vet.med. Beni-suef university, Egypt
fathykhlafalla@g.mail.com
Dina
ahmed
Barakat
Veterinary Medicine Directorate, Beni-Suef, Egypt.
rahaffarok@yahoo.com
10.21608/bvmj.2020.40633.1258
Abstract<br /> Because of rapid and busy life style, canned meat are widely consumed in Egypt. Therefore, the main objective of the current study was to evaluate the bacteriological status of canned meat marketed in Beni-Suef city. A total of 150 samples of canned meat represented by canned beef, corned beef, canned chicken sausage, canned chicken luncheon, canned luncheon and canned sausage (25 each) were examined for anaerobic plate count, Staphylococcus aureus count , enterococci count, total Clostridial count and isolation of clostridium perfringens. The highest prevalence of Clostridia were recorded in corned beef and canned sausage (60% each), while their lowest ones were in canned beef and canned poultry sausage (28% each). Twenty, 24%, 16, 12%, 24 and 24% of canned beef, corned beef, canned chicken sausage, canned chicken luncheon, canned luncheon and canned sausage, respectively exceeded the permissible limits of the E.O.S (2005) in relation to staph. aureus count. Enterococci could not be detected from canned chicken luncheon and canned sausage, while the levels of detection in canned beef, corned beef, canned chicken sausage, and canned luncheon were 12, 28, 4, and 12%, respectively. Cl. perfringens failed to be isolated from all the examined samples. It could be concluded that some of the examined samples were exceeding the local and international permissible limits for the examined bacteria, which may reflects under processing or poor storage conditions.
Keywords: Canned meat,canned sausage,Staph. aureus,C.perferingens,enterococci
https://bvmj.journals.ekb.eg/article_116466.html
https://bvmj.journals.ekb.eg/article_116466_5c549681bee8c5d3030d352864bac888.pdf
Benha University; Faculty of Veterinary Medicine
Benha Veterinary Medical Journal
1110-6581
2974-4806
39
1
2020
09
01
Isolation and identification of new sub-genotypes of virulent ND virus in broiler chickens in Qalybia governorate
159
164
EN
Asmaa
Ibrahim
Desouky
0000-0002-7597-6249
Avain and Rabbit disease department-veterinary medicine-benha univerisity
asmaaibrahim1293@yahoo.com
Ahmed
Eissa Mohamed
Saad
Avain and Rabbit disease department faculty of veterinary medicine benha university
ahmed.saad@fvtm.bu.edu.eg
Mohamed
Abdelgide
Elshorbagy
Avain and Rabbit Diseases department faculty of veterinary medicine benha university
mohamedelshorbagy@fvtm.bu.edu.eg
Samah
Abodalal
Newcastle disease department veterinary serum and Vaccine Researcher Institute, Agriculture Research Center, Abbasia, Cairo, Egypt
drsamahsaid@yahoo.com
10.21608/bvmj.2020.32932.1216
A B S T R A C T<br /> Newcastle disease (ND) is a highly contagious disease causing huge economic losses to the poultry industry due to high morbidities and mortalities. This study was aimed to isolate and characterize the new sub-genotype NDV strains in Qalybia Governorate during 2017 and 2019. Sixty eight samples were collected from Sixty eight broiler chicken flocks suffering from high mortality reach to 80% with distinctive clinical signs and post-mortem lesions of NDV. NDV strains isolated through inoculation in specific pathogen free (SPF) eggs and were identified by hemagglutination (HA) and hemagglutiation inhibition(HI) techniques. There were 16/20 (about 80%) of selected suspected farm were positive for NDV With HI test . These hemagglutinating agents were confirmed as NDV strains through molecular characterization using one step RT-PCR targeting the partial F-gene of NDV. Sequencing and phylogenetic analysis were successfully performed with eight NDV isolates which possessed the motif 112 R/K-R-Q-K/RR116 at the C-terminus of the F2 protein and F (phenylalanine) at residue 117, indicating that they are velogenic genotype. The present study revealed the circulation of NDV class II genotype VII in Qalybia chicken flocks.
Key words: Newcastle Disease,F protein Cleavage site,genotype VII
https://bvmj.journals.ekb.eg/article_116467.html
https://bvmj.journals.ekb.eg/article_116467_03e39414fb1bee4ecc9b63b561a0d352.pdf
Benha University; Faculty of Veterinary Medicine
Benha Veterinary Medical Journal
1110-6581
2974-4806
39
1
2020
09
01
Incidence of Psychotropic bacteria in frozen chicken meat products with special reference to Pseudomonas species
165
168
EN
Suzan
Hassan
Sheir
Animal Health research institute
alrehab2010exp@gmail.com
Hemmat
Mostafa
Ibrahim
Department of Food Hygiene, Faculty of Veterinary Medicine, Benha University. Animal Health
drhemmat01@yaoo.com
Mohamed
Ahmed
Hassan
Food Hygiene Department, Faculty of Veterinary Medicine, Banha university
mohamedhsn40@yahoo.com
Nahla
Ahmed
Shawky
Animal Health research institute, Shebin El koom branch
dr.nahlashawky@yahoo.com
10.21608/bvmj.2020.37744.1238
Total of hundred random samples of frozen chicken products represented by breast, thigh, nuggets and burger (25 of each) were collected from various supermarkets located in Menoufia government. To study the incidence of psychotropic bacteria with special reference to Pseudomonas species. The study revealed that mean values of Psychotropic count were 8.17×103± 1.42×103, 1.95×104± 2.06×104 , 3.63×104± 0.89×104 and 7.58×104± 1.16×104 respectively while the mean value of Psedomonas counts were 3.51×103± 0.76×103 , 6.29×103± 1.12×103,8.44×103± 1.85×103, 1.71×104± 0.36×104 respectively for examined frozen breast, thigh, nuggets and burger. It was obvious that 166 isolates were identified as P. acidovorans, P. aeruginosa, P. alcaligenes, P. cepacia, P. fluorescens, P. fragi, P. proteolytica, P. putida, P. putrefaciens, P. stutzeri, P. vesicularis. The prevalence of Psedomonas were 6, 1, 33, 8, 47, 10, 19, 3, 22, 2 and 15 from examined samples, respectively, where the highest contaminated product was the chicken burger 54/166(32.5%) Pseudomonas isolates and Ps. Fluorescens was the most detected isolate. The isolation of Pseudomonas species from food samples is highly significant Therefore, its presence should be prevented during earlier stages of food preparation.<br /> Key words: Psychotropic bacteria, frozen chicken meat products and Pseudomonas species.
Psychotropic bacteria,frozen chicken meat products,Pseudomonas Species
https://bvmj.journals.ekb.eg/article_116468.html
https://bvmj.journals.ekb.eg/article_116468_82cff13616199a079917fad7a29a4226.pdf
Benha University; Faculty of Veterinary Medicine
Benha Veterinary Medical Journal
1110-6581
2974-4806
39
1
2020
09
01
Bacteriological and molecular studies on Salmonella isolated from duckling farms at Kaliobia, Egypt
169
174
EN
Ibtehal
Saeed
Abdelaziz
0000-0002-0023-4873
Bacteriology. Veterinary Medicine Benha University. Egypt
ibtehalsaeed31@gmail.com
Ashraf
Awad
Abd El-Tawab
0000-0003-3172-87774
Bacteriology, Immunology, and Mycology, Faculty of Veterinary Medicine, Benha University, Benha, Egypt
ashrafabdeltwab@yahoo.com
Ahmed
Afifi
Maarouf
Chief Researcher of Food Hygiene, Animal Health Research Institute, Benha Branch
ahmedmaarouf@yahoo.com
Fatma
Ibrahim
Elhofy
Bacteriology, Immunology, and Mycology, Faculty of Veterinary Medicine, Benha University, Benha, Egypt
fatmaelhofy@yahoo.com
10.21608/bvmj.2020.38777.1242
This study was conducted on 21 commercial duckling farms (1- 20 days old) inspected to show Salmonella infection in different localities at Kaliobia Governorate. Samples were taken from diseased ducklings and freshly dead for bacteriological examination which resulted in, 94 samples were positive from 630 isolates, where 28 isolates from 33 diseased ducklings and 66 isolates from 72 freshly dead ducklings. Three serogroups of Salmonella were obtained by serological identification (Salmonella Typhimurium, Salmonella Enteritidis and Salmonella Blegdam). The antibiotic sensitivity tests for the isolated strains showed multiple antibiotic resistances (oxytetracycline; amoxicillin; ampicillin; streptomycin; erythromycin and trimethoprim/ sulphamethoxazol) but gentamycin, norfloxacin and ciprofloxacin are the most effective antibiotic on the isolated Salmonella and can be used for treatment of Salmonellosis in duck farms. PCR results appeared that, invA and stn genes were detected in all studied Salmonella isolates;pefA gene was detected in four out of five studied isolates but sefC genewas detected in two isolates only. Finally, isolated Salmonellae are virulant pathogens responsible for disease in ducklings resulting in high mortality and morbidity, gentamycin, norfloxacin and ciprofloxacin are the most proper antibiotics used for treatment of Salmonellosis in duck farms.
duckling,Salmonella,antibiotic resistant,Virulence genes
https://bvmj.journals.ekb.eg/article_116469.html
https://bvmj.journals.ekb.eg/article_116469_5bb585d1cd1bb97c34a51860998ff767.pdf
Benha University; Faculty of Veterinary Medicine
Benha Veterinary Medical Journal
1110-6581
2974-4806
39
1
2020
09
01
Mycotoxin residues in some fish products.
175
179
EN
M. S
Saad
Food Control Dept. Faculty of Veterinary Medicine, Benha University
M. A.
Hassan
Food Control Dept. Faculty of Veterinary Medicine, Benha University
F. S.
Hassanien
Food Control Dept. Faculty of Veterinary Medicine, Benha University
A. A.
Awud
Food Control Dept. Faculty of Veterinary Medicine, Benha University
d.ayaali511@gmail.com
10.21608/bvmj.2020.39473.1249
Abstract<br /> A grand of 90 random samples represented by smoked herring, canned sardines and frozen fish fillets (30 of each) were collected during their validity period from different areas in Menoufia Governorate to evaluate their mycotoxins quality. It was found that the average values of aflatoxin B1/kg in the examined fish products samples were 51.63 ±4.82µg/kg for smoked herring, 33.14±2.98µg/kg for canned sardine and 19.76 ±2.21µg/kg for frozen fish fillets.<br /> The mean values of aflatoxin B2/kg in the examined fish product samples were 37.29±3.75µg/kg for smoked herring, 20.81±3.02µg/kg for canned sardine and 14.57±1.90µg/kg for frozen fish fillets. The average value of aflatoxin G1/kg was 25.06 ±3.18µg/kg for smoked herring, 14.42±1.96µg/kg for canned sardine and 9.65±1.32µg/kg for frozen fish fillets. The average values of aflatoxin G2/kg in the examined fish products samples were 16.22±1.39µg/kg for smoked herring, 11.29±0.92µg/kg for canned sardine and 4.46±0.53µg/kg for frozen fish fillets. Whereas, the mean values of Ochratoxin A/kg were 6.52±0.74µg/kg in the examined smoked herring samples, 5.60±0.61µg/kg in the examined samples of canned sardine and 3.24±0.39µg/kg in frozen fish fillets. Moreover, it was found that aflatoxin B1 was the predominant mycotoxin that detected in the examined fish products samples particularly smoked herring.
Keywords: Aflatoxin,Ochratoxin,smoked herring and fish products
https://bvmj.journals.ekb.eg/article_116470.html
https://bvmj.journals.ekb.eg/article_116470_0e378e730997983612556097109e9402.pdf
Benha University; Faculty of Veterinary Medicine
Benha Veterinary Medical Journal
1110-6581
2974-4806
39
1
2020
09
01
Antimicrobial effects of some essential oils and honey on some pathogenic bacteria in chicken meat
180
183
EN
shimaa
mohamed
Food hygiene department
shimaa.rashad12@gmail.com
Amany
Salem
Professor of Meat Hygiene, Food Control Department,
Faculty of Veterinary Medicine, Benha University
dr_amany40@yahoo.com
10.21608/bvmj.2020.25900.1190
The present study was designed to investigate antimicrobial activity of garlic (Allium sativum), thyme (Tthymus vulgaris) oils at concentrations (1%) and honey (10%) in chicken meat. A total of 2000g of the fresh chicken meat samples were divided into two groups (A&B) the firist group was inoculated with E. coli (106 cfu/g) and the second one with S. aureus (106 cfu\ g) strains. Both of them were subdivided into untreated (control) and treated ones with garlic (1%), thyme (1%) oils and honey (10%), respectively. All samples were stored at 4°C and subjected to sensory and bacteriological evaluations after 3 hours, 1st, 2nd, 3rd, 4th and 5th day of cold storage. Sensory attributes of different treated chicken meat samples were mostly improved with honey (10%) followed by thyme oil (1%) then garlic oil (1%) when compared with control. Honey (10%) group give the best effectiveness of both pathogens reduction (100%) at 3th, 4th and 5th days with a significant advantage in extend shelf-life of refrigerated chicken meat compared to all groups specially control ones. Generally, some natural compounds proved to be efficient in improve safety and shelf-life. Generally, essential oils & honey proved to be efficient natural preservatives which more safe to human than chemical ones.
Honey,Essential oils,E.coli,S.aureus,Chicken meat
https://bvmj.journals.ekb.eg/article_116471.html
https://bvmj.journals.ekb.eg/article_116471_e5c45f4ea238edf4ca7fd5a878aaca04.pdf
Benha University; Faculty of Veterinary Medicine
Benha Veterinary Medical Journal
1110-6581
2974-4806
39
1
2020
09
01
Assessment of some pathogens in retailed poultry meat
184
188
EN
shimaa
mohamed
Food hygiene department
shimaa.rashad12@gmail.com
Amany
Salem
Professor of Meat Hygiene, Food Control Department,
Faculty of Veterinary Medicine, Benha University
dr_amany40@yahoo.com
Ahmed
Maarouf
Animal Health Research Institute, "Benha branch "ARC
10.21608/bvmj.2020.25898.1189
The present study was performed on 150 random samples of Fresh, half cooked (nuggets) and cooked (grilled) chicken meat (50 of each) were purchased from retail chicken butchers, different supermarkets and restaurants in Kaliobia Governorate, Egypt, to evaluate their bacterial quality and safety. Bacteriological examination of chicken meat samples revealed that, the mean values of APC, Enterobacteriaceae, Coliform, Staphylococcus and S. aureus (cfu /g) were 9.88×105 ±0.61×105; 3.36×10 4±0.26 ×104; 1.43×10 4±0.16 ×104; 2.71×103 ±0.17 ×103 and 2.05×103 ±0.24 ×103 in fresh, 5.48×104±0.23×104; 3.15×102±0.15 ×102; 1.00×102±0.08 ×102; 1.73×102±0.11 ×102 and 1.26×102±0.19 ×102 in half cooked and 4.50×104±0.22×104; 2.06×102 ±0.13 ×102; 1.09×102 ±0.10 ×102; 1.05×102 ±0.09 ×102 and 1.02×102 ±0.15 ×102 cooked chicken meat samples respectively. Moreover, the incidence of E. coli were 14% in fresh O55: H7(6%); O125: H21(4%); O111: H2 (2%) and O146: H21(2%); 6% in half cooked O55: H7(2%); O125: H21(2%) and O111: H2(2%) and 6% in cooked chicken meat samples O55: H7(4%) and O111: H2(2%). Meanwhile, the incidence of Coagulase positive S. aureus were (38%) (26%) and (20%) in fresh, half cooked and cooked chicken meat samples. According to SET- RPLA test, 7 strains out of 12 examined strains were enterotoxigenic. In addition, PCR showed that S. aureus enterotoxins sea, seb, sec, sed, see were detected in 10 S.aureus isolate from samples.
Poultry meat,PCR,Food poisoning,SET- RPLA test
https://bvmj.journals.ekb.eg/article_116472.html
https://bvmj.journals.ekb.eg/article_116472_063ad04f81530c54dcd8706e0a6a324a.pdf
Benha University; Faculty of Veterinary Medicine
Benha Veterinary Medical Journal
1110-6581
2974-4806
39
1
2020
09
01
Effect of Flavomycin and Synbiotic on experimentally-infected common carp with Pseudomonas aeruginosa
189
193
EN
Eman
M
Soror
11 moshtohor
eman.sorour@fvtm.bu.edu.eg
10.21608/bvmj.2020.20660.1173
This study investigated the antibacterial effect of flavomycin and synbiotic against Pseudomonas aeruginosa (p. aeruginosa) beside the weight gain and hemato-biochemical changes in common carp. Apparently healthy 150 common carp weighing 110 ± 10 g were divided into six equal groups. The 1st group received basal diet without any additives (control group). The 2nd group received basal diet with 8 mg/kg diet flavomycin for 30 days. The 3rd group received basal diet with 3 mg/kg diet synbiotic for 30 days. Fish in 4th, 5th and 6th groups were experimentally infected with P. aeruginosa. The 4th group was left untreated. The5th and 6th groups were treated with 8 mg/kg diet flavomycin or 3 mg/kg diet symbiotic, respectively for 5 days. Swabs were collected to reisolate p. aeruginosa. Blood samples were collected for hemato-biochemical analysis. Healthy fish received flavomycin or synbiotic revealed significant increase in body weight gain, RBCs, Hb, PCV with non-significant increase in total protein, albumin, globulin, A/G ratio, AST, ALT, urea, creatinine. Infected fish with P. aeruginosa showed tail and fin rot, hemorrhagic ulcerative skin, superficial ulcers beside mortality rate up to 32% with significant decrease in body weight, RBCs, HB, PCV %, total protein, albumin, globulin and significant elevation in AST, ALT, ALP, urea and creatinine. Infected fish treated with flavomycin or synbiotic improved the clinical signs, reduced mortality rate to 8% and 12%, respectively, and decreased rate of infection. In conclusion, flavomycin or synbiotic has effective antibacterial effect against P. aeruginosa.
common carb,Flavomycin,hematobiochemical,Pseudomonas aeruginosa,synbiotic
https://bvmj.journals.ekb.eg/article_116473.html
https://bvmj.journals.ekb.eg/article_116473_9247e3ec6ba7c236032f410dba893146.pdf