Benha University; Faculty of Veterinary Medicine
Benha Veterinary Medical Journal
1110-6581
2974-4806
30
1
2016
03
01
Effect of Spirulina platensis as feed supplement on growth performance, immune response and antioxidant status of mono-sex Nile Tilapia (Oreochromis niloticus)
1
10
EN
Shimaa
A.
Amer
Department of Nutrition & Clinical Nutrition, Faculty of Veterinary Medicine, Zagazig University, Egypt
10.21608/bvmj.2016.31332
To evaluate the effect of Spirulina platensis inclusion in diets for Nile tilapia fingerlings, a completely<br />randomized experimental design was developed with 4 treatments and three replicates. Diets with 4<br />inclusion levels (control diet (Sp0), 0.5% (Sp0.5), 1% (Sp1) and 1.5% (Sp1.5)) were prepared. 180 Nile<br />tilapia fingerlings with average initial weight of 8.46±0.17 g were assigned to twelve experimental tanks.<br />The experiment lasted for ten weeks. At the end of the experiment; growth performance, the whole body<br />composition was determined and serum samples for lysozyme, IgG, IgM values and catalase activity<br />were collected and antioxidant status of Nile tilapia muscle were determined. The results showed that<br />group supplemented with 1% S. Platensis have higher body weight and lower FCR than the control<br />group and other groups. S. platensis inclusion increased activity of glutathione reductase, and reduce<br />MDA formation. Levels of lysozyme, IgG and catalase activity were higher in S. platensis supplemented<br />groups but don’t have significant effect on IgM level. In conclusion, Spirulina platensis supplementation<br />increased antioxidant protective capacities. It also affected some innate and humoral immunity<br />parameters as well as don’t have negative effect on fish growth
Nile tilapia,Spirulina platensis,Growth performance,antioxidant status,innate and humoral immunity
https://bvmj.journals.ekb.eg/article_31332.html
https://bvmj.journals.ekb.eg/article_31332_ab3bb0a7b293335d8b5afe3e2a4f555d.pdf
Benha University; Faculty of Veterinary Medicine
Benha Veterinary Medical Journal
1110-6581
2974-4806
30
1
2016
03
01
Prevalence and antimicrobial susceptibility pattern of Staphylococcus aureus isolated from dairy cattle's subclinical mastitis in EL-Sharkia Governorate
11
19
EN
Ashraf
A.
Abd El-Tawab
Bacteriology, Immunology and Mycology Dep., Fac. of Vet. Med., Benha Univ.
Fatma
I.
El Hofy
Bacteriology, Immunology and Mycology Dep., Fac. of Vet. Med., Benha Univ.
Ahmad
M.
Ammar
Bacteriology, Immunology and Mycology Dep., Fac. of Vet. Med., Zagazig Univ.
Mokhtar
Abd El-Hakeem
Sleim
Animal Health Research Institute (Zagazig branch)
Heba
S. S.
Salem
Animal Health Research Institute (Zagazig branch)
10.21608/bvmj.2016.31333
Subclinical mastitis is a disease of major economic importance to dairy industry causing reduced milk<br />quality and loss in its production. Therefore, the present study was carried out for isolation and<br />identification the main pathogen responsible for this disease and detection the drug of choice. A total of<br />475 milk samples of lactating dairy cows were collected from different localities in El-Sharkia<br />Governorate, and subjected to physical, chemical tests (California mastitis test (CMT) as well as Somatic<br />cell count (SCC), bacteriological examination and antimicrobial susceptibility pattern. The prevalence<br />of subclinical mastitis at cow level was 21% (100/475). Accurately 80 Staphylococcal isolates were<br />identified with prevalence of 61% S. aureus through beta hemolysis and coagulase positive. The<br />obtained S. aureus isolates were highly sensitive to vancomycin, gentamicin, ciprofloxacin and<br />chloramphenicol with percentages of 93.4%, 83.6%, 82% and 80.3%, respectively. Meanwhile, high<br />frequency of resistance was observed to oxacillin, erythromycin and tetracycline, with percentages of<br />59%, 55.7%, and 47.5%, respectively. Therefore, the main choice drugs for subclinical mastitis were<br />vancomycin and gentamicin
Subclinical mastitis,California mastitis test,Antibiotic Susceptibility
https://bvmj.journals.ekb.eg/article_31333.html
https://bvmj.journals.ekb.eg/article_31333_76757d9f0008c348e1370d0e427fd988.pdf
Benha University; Faculty of Veterinary Medicine
Benha Veterinary Medical Journal
1110-6581
2974-4806
30
1
2016
03
01
Effect of prebiotic on the immune status of Oreochromis niloticus
20
28
EN
Ashraf
Awad
Abd El Tawab
Bacteriology, Immunology and Mycology Department Faculty of Veterinary Medicine, Benha University
Fatma
Abd-Allah
El-Hofy
Bacteriology, Immunology and Mycology Department Faculty of Veterinary Medicine, Benha University
Ahmed
Mohamed
Ammar
Bacteriology, Immunology and Mycology Department Faculty of Veterinary
Medicine, Zagazig University
Osama
Abd El-Rahman
Saleh
Central Lab for Aquaculture Research, Egypt
Heba
Ahmed
Tolba
Central Lab for Aquaculture Research, Egypt
10.21608/bvmj.2016.31335
A total number of 180 Nile-tilapia (Oreochromis niloticus) were divided into four equal<br />groups; the first one served as control fed on basal non-treated diet. The second group fed on<br />diet supplemented with 0.5 ml prebiotic βpolo /kg basal fish diet, the third group on diet<br />supplemented with 1 ml prebiotic βpolo/kg basal fish diet, and the fourth group fed on diet<br />supplemented with 1.5 ml prebiotic β polo/kg basal fish diet for 40 days. The result elevated<br />that, the growth performance was significantly higher in all treatments than in the control group<br />and the highest increased was in the group received 1.5 ml prebiotic βpolo /kg basal fish diet.<br />The hematocrit (HC) values, nitroblue-tetrazolium (NBT) and lysozyme activities were<br />increased significantly in all treated groups than the control and the highest increased was in<br />the group received 1.5 ml prebiotic βpolo /kg basal fish diet. The percent level of protection<br />among the three treated groups after challenge infection using Pseudomonas flourescence (Ps.<br />flourescence) (0.5 ml of culture suspension of pathogen containing 107 bacteria ml-1) was<br />higher than control and the highest record was in the group of received 1.5 ml prebiotic βpolo<br />/kg basal fish diet
prebiotic,Immune Response,Ps. flourescence,Nile tilapia
https://bvmj.journals.ekb.eg/article_31335.html
https://bvmj.journals.ekb.eg/article_31335_61ccf8813ffc63cb619768fd306df412.pdf
Benha University; Faculty of Veterinary Medicine
Benha Veterinary Medical Journal
1110-6581
2974-4806
30
1
2016
03
01
Biochemical alterations of inflammatory markers in experimentally induced diabetes in rats
29
38
EN
El-Regily
A.
F.Y.
Department of Biochemistry, Faculty of Veterinary Medicine, Damanhour University
El-Hindawy
U.
M.
Department of Biochemistry, Faculty of Veterinary Medicine, Damanhour University
Sadek
K.
M.
Department of Biochemistry, Faculty of Veterinary Medicine, Damanhour University
Abd El-Maksoud
H.A.
A.
Department of Biochemistry, Faculty of Veterinary Medicine, Benha University
10.21608/bvmj.2016.31341
This study was evaluated the protecting effect of rutin on the glycemic condition, lipid profile, in<br />streptozotocin (STZ)-induced experimental diabetic in rats. With respect to an orally administrated rutin<br />on serum glucose, the result is showing a significant decrease in glucose concentration when compared<br />to diabetic group, While, a significant increase in insulin concentration when compared to diabetic.<br />Moreover, the results revealed that, an administration of streptozotocin (STZ) showing significant<br />increase in triacylglycerol, total cholesterol, LDL-c and VLDL-C concentration when compared with<br />control group, while, showing significant decrease in HDL-C concentration and serum AST and ALT<br />activity when compared to the control group. The data revealed also significant increase in serum LMDA<br />concentration. While, significant decrease in serum calcium concentration when compared to the<br />control group
streptozotocin,Rutin,glucose,Insulin,L-MDA,calcium
https://bvmj.journals.ekb.eg/article_31341.html
https://bvmj.journals.ekb.eg/article_31341_2ebdf4744fcadfcb7ec2b646367a5ab7.pdf
Benha University; Faculty of Veterinary Medicine
Benha Veterinary Medical Journal
1110-6581
2974-4806
30
1
2016
03
01
Biochemical effect of β-hydroxy-β-methylbutyrate calcium and creatine supplementations on some blood parameters, pro-inflammatory cytokines and growth performance of broiler chicks
39
50
EN
Mohamed
K.
Mahfouz
Department of Biochemistry, Faculty of Veterinary Medicine, Benha University, Egypt
Kamel
A. M.
Zyan
Department of Poultry Diseases, Faculty of Veterinary Medicine, Benha University, Egypt
kamel_zyan@yahoo.com
Hossam
H.
Abdel-Hamid
Department of Biochemistry, Faculty of Veterinary Medicine, Benha University, Egypt
10.21608/bvmj.2016.31342
One hundred and sixty-five one-day old Cobb broiler chicks were allocated into 3 groups to investigate the<br />effect of dietary 0.1% β-hydroxy- β-methylbutyrate calcium (HMB) and 0.1% creatine on some blood<br />parameters, pro-inflammatory cytokines and growth performance parameters in terms of total feed intake<br />(TFI), total body weight gain (TBWG), total body weight and feed conversion ratio (FCR) during certain<br />time intervals (21<sup>st</sup> day and 42<sup>nd</sup> day) versus control group (0 treatment). Some biochemical changes were<br />recorded at the 42<sup>nd</sup> day among creatine supplemented group in the form of significantly high results of both<br />triglycerides and T3 in group 3 (creatine) than that of the control group on the same day at P value > 0.05.<br />HMB supplemented group showed significantly lower results of ALT (mean) than that of control group at<br />(P value > 0.05) on the 21<sup>st</sup> day which became insignificant at the 42nd day. Dietary 0.1% HMB-Ca recorded<br />better growth performance parameters in terms of TFI (4.527), TBWG (2.515), TBW (2.555) and TFCR<br />(1.8) if compared to creatine supplemented group of (4.664, 2.508, 2.549 and 1.86) respectively and the<br />control group of TFI equaled to 4.7432, TBWG equaled to 2.156, TBW equaled to 2.1987 and TFCR<br />equaled to 2.2 at the end of the experiment. HMB and creatine supplementations had no effect on other<br />blood parameters, viz. AST, urea, creatinine, total protein, FFA, T4, T3/T4 ratio, TSH, uric acid, cholesterol,<br />HDL and LDL, and pro-inflammatory cytokines namely IL-6, IL-2 and TNF-α.
HMB-Ca,Creatine,broiler chicks,TFI,FCR,TBWG,TBW
https://bvmj.journals.ekb.eg/article_31342.html
https://bvmj.journals.ekb.eg/article_31342_9f373e9facd1e5ed9fcf1b07828f965e.pdf
Benha University; Faculty of Veterinary Medicine
Benha Veterinary Medical Journal
1110-6581
2974-4806
30
1
2016
03
01
Monitoring the bacterial contamination during different stages of beef carcass preparation at Beni-Suef abattoir, Egypt
51
58
EN
Fathy
A.
Khalafalla
Food Hygiene Department, Faculty of Veterinary Medicine. Beni-Suef University, Beni-Suef, 62511, Egypt
Fatma
H.M.
Ali
Food Hygiene Department, Faculty of Veterinary Medicine. Beni-Suef University, Beni-Suef, 62511, Egypt
Abdel-Rahim
H.A.
Hassan
Food Hygiene Department, Faculty of Veterinary Medicine. Beni-Suef University, Beni-Suef, 62511, Egypt
abdelrahim@vet.bsu.edu.eg
Kareem
A.
El-Feky
Food Hygiene Department, Faculty of Veterinary Medicine. Beni-Suef University, Beni-Suef, 62511, Egypt
10.21608/bvmj.2016.31345
In an attempt to monitor the levels of bacterial contamination during different stages of beef carcass<br />preparation at Beni-Suef abattoir, Egypt, 20 beef carcasses were examined. Each carcass was<br />represented by swabs from the abdomen, neck, shoulder, thigh and thorax, collected after dehiding, after<br />evisceration and after complete preparation. Each swab was examined for the total mesophilic,<br />psychrophilic and staphylococcal counts, MPN of coliforms, fecal coliforms and E. coli. The mesophilic<br />counts in the examined swabs of abdomen, neck, shoulder, thigh and thorax after dehiding were 3×10<sup>6</sup>,<br />3×10<sup>6</sup>, 5×10<sup>6</sup>, 7×10<sup>5</sup>, 3×10<sup>6</sup>, while after evisceration, they changed to 2×10<sup>7</sup>, 5×10<sup>6</sup>, 4×10<sup>6</sup>, 5×10<sup>5</sup>, 2×10<sup>6</sup><br />CFU/cm<sup>2</sup>, respectively. Moreover, they accounted for 5×10<sup>5</sup>, 5×10<sup>6</sup>, 7×10<sup>6</sup>, 2×10<sup>5</sup>, 5×10<sup>6</sup> CFU/cm<sup>2</sup> after<br />preparation, respectively. The staphylococcal counts in the examined swabs were 7×10<sup>2</sup>, 7×10<sup>2</sup>, 8×10<sup>2</sup>,<br />7×10<sup>2</sup>, 8×10<sup>2</sup> after dehiding, 6×10<sup>2</sup>, 9×10<sup>2</sup>, 6×10<sup>2</sup>, 6×10<sup>2</sup>, 4×10<sup>2</sup> CFU/cm<sup>2</sup> after evisceration,<br />respectively. While, they were 4×10<sup>2</sup>, 9×10<sup>2</sup>, 10×10<sup>2</sup>, 6×10<sup>2</sup>, 3×10<sup>2</sup> CFU/cm<sup>2</sup> after preparation,<br />respectively. As regard to coliforms (MPN) in the examined swabs after dehiding, they were 2×10,<br />4×10<sup>2</sup>, 2×10<sup>2</sup>, 4×10<sup>2</sup>, 2×10<sup>2</sup>, while 9, 3×10<sup>2</sup>, 1×10<sup>2</sup>, 3×10<sup>2</sup>, 1×10<sup>2</sup> after evisceration, and <3, 3×10<sup>2</sup>,<br />8×10, 2×10, 3×10 m.os./cm<sup>2</sup> after preparation, respectively. The sources of contamination with the<br />determined bacteria were discussed throughout the study
abattoir,Bacterial contamination,APC,beef,coliforms MPN
https://bvmj.journals.ekb.eg/article_31345.html
https://bvmj.journals.ekb.eg/article_31345_44ef810de2c2afd751a951629fd285ac.pdf
Benha University; Faculty of Veterinary Medicine
Benha Veterinary Medical Journal
1110-6581
2974-4806
30
1
2016
03
01
Comparison of modified decontamination methods with culture systems for primary isolation of Mycobacterium bovis from bovine tissues
59
67
EN
Nasr
E.
A.
Department of Bacterial Diagnostic Products (Tuberculosis), Veterinary Serum and Vaccine Research Institute, Abassia, Cairo, Egypt
Marwah
M.
Department of Bacterial Diagnostic Products (Tuberculosis), Veterinary Serum and Vaccine Research Institute, Abassia, Cairo, Egypt
Abdel Rahman
M.
Department of Bacterial Diagnostic Products (Tuberculosis), Veterinary Serum and Vaccine Research Institute, Abassia, Cairo, Egypt
Shafeek
H.
General Organization of Veterinary Service, Giza
10.21608/bvmj.2016.31346
A definitive diagnosis of M. bovis infection in animals depends on the isolation of the organism, as the<br />optimum condition for the primary isolation of M. bovis infection has not been determined. In this study<br />a total number of 2550 cattle from different governorates in Egypt were tested by Single Intradermal<br />Cervical (SID) tuberculin skin test. Positive reacted cattle (no= 42, 1.6%) were slaughtered and<br />suspected lesions were collected, these samples were decontaminated by using three different methods,<br />which were 0.75 % Hexadecyl Pyridinium Chloride (HPC), 5% oxalic acid and 6 % sulphuric acid.<br />Isolation of mycobacteria from 42 samples by using these decontaminated methods were of 34 (80.9<br />%), 30 (71.4 %) and 28 (66.6 %), respectively. So it could be concluded that using HPC method as<br />decontaminated agent revealed high rate of isolation of mycobacteria with less contamination (7.1 %)<br />than the other two methods
Mycobacterium bovis – HPC- decontamination – oxalic acid – sulphuric acid
https://bvmj.journals.ekb.eg/article_31346.html
https://bvmj.journals.ekb.eg/article_31346_a2e414edfd4e589301f1ecc0b4eacad6.pdf
Benha University; Faculty of Veterinary Medicine
Benha Veterinary Medical Journal
1110-6581
2974-4806
30
1
2016
03
01
Preliminary studies on E. Coli implicated in avian Colibacillosis with reference to their antibiotic resistance profiles
68
77
EN
Ashraf
A.
Abd El Tawab
Bacteriology, Immunology and Mycology Dept. Fac. Vet. Med., Benha Univ.
Fatma
I.
El-Hofy
Bacteriology, Immunology and Mycology Dept. Fac. Vet. Med., Benha Univ.
Ahmed
M.
Ammar
Bacteriology, Immunology and Mycology Dept., Fac. Vet. Med. Zagazig Univ.
Mokhtar
Abdel Hakeem
Animal Health Research Institute (Zagazig branch)
Noha
M.
Abdel Galil
Animal Health Research Institute (Zagazig branch)
10.21608/bvmj.2016.31348
Colibacillosis constituted as one of the most important disease condition affecting poultry with<br />significant implications on production. Herein, examination of 451 organ samples including trachea,<br />lung, heart blood, liver and spleen collected from freshly slaughtered chickens using standard methods<br />for isolation and identification of E. coli showed that 236 samples were positive for E. coli with an<br />incidence rate (52%). Twenty-five E. coli strains were serogrouped and 6 different serogroups were<br />successfully identified, the most prevalent serogroup was O78 (20%), followed by O157 and O27 with<br />incidence rate (16%) for each, O168 and O125 (12%) each. and finally O115 (8%), in addition to 4<br />untypable strains. The antibiotic susceptibility of 60 isolates against ten antimicrobials was performed<br />by disc diffusion method and the results revealed that gentamicin and colistin were the most effective<br />antibiotics on the isolates. On the other hand, all isolates exhibited absolute resistance to erythromycin<br />and rifampicin, followed by high level of resistance ranging from (75-95%) for ciprofloxacin,<br />sulfamethoxazole–trimethoprim, cefoxtin, chloramphenicol, doxycycline and amoxicillin- clavulanic<br />acid. All isolates exhibited multidrug resistance phenotypes
Escherichia coli,Biochemical identification,Serogrouping,Antibiotic resistance
https://bvmj.journals.ekb.eg/article_31348.html
https://bvmj.journals.ekb.eg/article_31348_b9f7da65fdad13a57ecc394606d32c7f.pdf
Benha University; Faculty of Veterinary Medicine
Benha Veterinary Medical Journal
1110-6581
2974-4806
30
1
2016
03
01
Efficacy of the E. Coli whole cell lysates for improving immunogenic quality of the local entero-3 vaccine
78
85
EN
Effat
L.
El-Sayed
Veterinary Serum and Vaccine Researches Institute, Abbassia, Cairo, Egypt
Eman
S. A.
Zaki
Veterinary Serum and Vaccine Researches Institute, Abbassia, Cairo, Egypt
A.
A.
El-Kholy
Veterinary Serum and Vaccine Researches Institute, Abbassia, Cairo, Egypt
Sleem
S.
Salama
Central Laboratory for Quality control of Veterinary Biologies, Abbassia, Cairo, Egypt
10.21608/bvmj.2016.31349
Bacterial cell lysates of E. coli strains (K99 and O 157) were prepared and used as vaccinal antigens<br />added to locally prepared Entero-3 inactivated vaccine components aiming to improve the immune<br />response of calves against E. coli. Three groups of apparently healthy crossbred, three calves per group,<br />were vaccinated with two doses (4ml I/M /dose), three weeks apart as follow: the first group (G.Ι) was<br />vaccinated by local inactivated Entero-3 vaccine (Rota, Corona and E. coli K99). The second group<br />(G.ΙΙ) was vaccinated by vaccine containing Rota, Corona and cell lysate of E. coli K99 & E. coli O157.<br />The third one (G.ΙΙΙ) was vaccinated by vaccine containing Entero-3 vaccine component and cell lysate<br />of E-coli K99 & E-coli O157, and the fourth group kept unvaccinated served as control. The antibody<br />response in sera of vaccinated calves measured by serum neutralization test (SNT) and ELISA, which<br />revealed that all types of the prepared vaccines induced a protective antibody titres. Entero-3 vaccine<br />containing cell lysate of E-coli strains induced higher response than the other two vaccines. In<br />conclusion, adding of E. coli strains lysates as a vaccinal antigen to the locally prepared Entero-3<br />vaccine, provided a formulation of Entero -3 vaccine that conferred a good immune response of calves<br />against collibacellosis
Entero-3,E. coli,vaccine,lysate,Collibacellosis,calves
https://bvmj.journals.ekb.eg/article_31349.html
https://bvmj.journals.ekb.eg/article_31349_b8b445522ea25cc02d4de0f69a15a04b.pdf
Benha University; Faculty of Veterinary Medicine
Benha Veterinary Medical Journal
1110-6581
2974-4806
30
1
2016
03
01
Prevalence and molecular analysis of anaplama and piroplasmid species infecting buffaloes in qualyubia governorate, Egypt
86
96
EN
Nagwa
E.
A.
Parasitology Department, Faculty of Veterinary medicine, Benha University
Elmadawy
R.
S.
Parasitology Department, Faculty of Veterinary medicine, Benha University
Ghanem
M.
M.
Animal Medicine Department, Faculty of Veterinary medicine, Benha University
ELdiarby
A.
S.
Veterinary Teaching Hospital, Faculty
of Veterinary Medicine, Benha University
10.21608/bvmj.2016.31350
This study aimed to investigate the prevalence of Anapalsma and piroplasmid species infecting buffaloes<br />with regard to season, age and sex. To achieve this aim, blood samples from a total of 530 apparently<br />healthy and clinically suspected buffaloes in Qualyubia governorate, Egypt were collected throughout<br />the period from June 2013 to May 2014. The blood samples were smeared, stained with Giemsa and<br />examined by conventional microscope. Moreover, PCR assay was applied to amplify DNA of Babesia<br />and Theileria sp. in 100 microscopically negative buffaloes' blood samples. The microscopic<br />investigation identified the infection in 37.92% of the examined buffaloes. Anaplasma marginale was<br />the most prevalent species (30.94%) followed by Babesia sp. (26.60%) and Theileria annulata (2.08%).<br />The most frequent clinical signs observed during the course of disease, in addition to the effect of<br />seasons, age and sex on the prevalence of infection were presented in the study. PCR assay proved to<br />be the most reliable method for the diagnosis of piroplasmid infections as compared to light microscopy,<br />where it was able to detect Babesia and Theileria sp. DNA in 25% and 12% with 5% mixed in the<br />microscopically negative blood samples of buffaloes respectively. The high prevalence of infection in<br />buffaloes highlights the need for establishment of a consolidated control program to overcome the<br />economic losses caused by these parasites
Buffalo,Piroplasmosis,prevalence,PCR
https://bvmj.journals.ekb.eg/article_31350.html
https://bvmj.journals.ekb.eg/article_31350_31d00d2f025aa3725e2282a1ea9527c9.pdf
Benha University; Faculty of Veterinary Medicine
Benha Veterinary Medical Journal
1110-6581
2974-4806
30
1
2016
03
01
Rapid tests for detection of enrofloxacin residues in liquid milk
97
103
EN
Hamdi
A.
Mohammed
Department of Food Control, Faculty of Veterinary Medicine, Benha University
Adham
M.
Abdou
Department of Food Control, Faculty of Veterinary Medicine, Benha University
Amal
M.
Eid
Animal Health Research Institute, Tanta Branch
amalaeidvet@gmail.com
Asem
M.
Zakaria
Department of Food Control, Faculty of Veterinary Medicine, Aswan University
10.21608/bvmj.2016.31351
A total of 234 randomly selected milk samples from different sources (110, 50, 34 and 40 samples) from<br />each of (market milk, collecting centers, bulk tank and UHT milk samples; respectively) were analyzed<br />for detection the quinolones residues by rapid quinolones detection strip test. Results revealed that<br />17.3%, 14% and 20.6% of milk samples were positive for the quinolones residues for market milk,<br />collecting centers and bulk tank milk samples; respectively, while not detected in UHT milk samples.<br />The positive milk samples were subjected to HPLC analysis for quantitative detection of enrofloxacin<br />residues, which were not detected in market milk and collecting centers milk samples while detected in<br />one sample from bulk tank milk samples. The mean value of 2.94 ppb that not exceeded the maximum<br />residual limit set by different international standards. Public health importance of enrofloxacin residues<br />was discussed
Rapid tests,Enrofloxacin,Liquid Milk
https://bvmj.journals.ekb.eg/article_31351.html
https://bvmj.journals.ekb.eg/article_31351_eca0ff0ab430f03ca458deabf97399fb.pdf
Benha University; Faculty of Veterinary Medicine
Benha Veterinary Medical Journal
1110-6581
2974-4806
30
1
2016
03
01
Incidence of E. coli in some meat products
104
108
EN
Fatin
M.
Hassanien
Department of Food Control, Faculty of Veterinary Medicine, Benha University
Shiamaa
M.
Nada
Animal Health Research Institute, Shebin- Elkom Branch
Alaa
M.
Abd-Elsattar
Animal Health Research Institute, Shebin- Elkom Branch
10.21608/bvmj.2016.31353
A grand total of 100 random samples of meat products represented by beef burger and kofta (50 of each)<br />100g were collected from different shops and hyper supermarkets in different districts in Menofia<br />governorate. The incidence of E. coli in the examined samples of meat products were 28% and 36 %for<br />beef burger and kofta, respectively. Moreover, the isolated serotypes of E. coli from the examined<br />samples of meat products were O<sub>26</sub>: H<sub>11</sub>, O<sub>86</sub>, O<sub>55</sub>: H<sub>7</sub>, O<sub>104</sub>: H<sub>4</sub>, O<sub>111</sub>: H<sub>4</sub>, O<sub>114</sub>: H<sub>21</sub>, O<sub>119</sub>: H<sub>4</sub>, O<sub>124</sub>, O<sub>125</sub>:<br />H<sub>21</sub>, O<sub>167</sub>: H<sub>21</sub> and O<sub>128</sub>: H<sub>2</sub> with various percentages, Also, application of sensitivity test on all E. coli<br />strains indicated that most of them were multiple antimicrobial resistance (MAR) where the MAR index<br />equal 0.515. The public health significance of the isolated organisms from meat products was discussed<br />as well as some recommendations to ensure safety and quality of meat products reach to consumers
E. coli,meat products,Multiple antimicrobial Resistances
https://bvmj.journals.ekb.eg/article_31353.html
https://bvmj.journals.ekb.eg/article_31353_8ac30a843b24d669e9886c1ff4f8473c.pdf
Benha University; Faculty of Veterinary Medicine
Benha Veterinary Medical Journal
1110-6581
2974-4806
30
1
2016
03
01
Bacterial and Chemical quality of Frozen Chicken Meat Received at Governmental Hospital modern
109
117
EN
Fatin
M.
Hassanien
Department of Meat Hygiene, Faculty of Veterinary Medicine, Benha University
Rasha
A.
El-Sabagh
Department of Meat Hygiene, Faculty of Veterinary Medicine, Benha University
Marionet
Z.
Nassief
Animal Health Research Institute, Benha Branch
Mohammed
S.
Refat
Hospital Benha University
10.21608/bvmj.2016.31355
This study was conducted to evaluate the quality of frozen chicken meat received at governmental<br />hospital in kalyobia governorate, Egypt. A total of 100 samples were taken from chicken breast and<br />thigh (50 of each), randomly for bacteriological and chemical examination. The average of APC,<br />coliform and psychrotrophes counts were 3.78×10<sup>6</sup>±0.93±10<sup>6</sup>/g, 2.07×10<sup>3</sup>±0.60×10<sup>3</sup>/g and<br />5.71×10<sup>6</sup>±1.44×10<sup>6</sup>/g for breast and 4.38×10<sup>6</sup>±0.59×10<sup>6</sup>/g, 2.61×10<sup>3</sup>±0.60×10<sup>3</sup>/g and<br />4.59×10<sup>6</sup>±1.26×10<sup>6</sup> /g for thigh, respectively. Chemical keeping quality tests represented by pH, TVB/N<br />(mg/100g) and TBA (mg/kg) indicated that the mean values were 5.84±0.10, 18.99±0.59 and 0.68±0.01<br />for chicken breast and 5.91±0.11, 19.28±0.60 and 0.73±0.02 for chicken thigh, respectively. The<br />recommended points were discussed
Frozen chicken meat,APC,Coliform,Psychrotrophes,pH,TVB/N and TBA
https://bvmj.journals.ekb.eg/article_31355.html
https://bvmj.journals.ekb.eg/article_31355_70c9a259cf290208c27eb66ee0542d41.pdf
Benha University; Faculty of Veterinary Medicine
Benha Veterinary Medical Journal
1110-6581
2974-4806
30
1
2016
03
01
Bacteriological and immunological studies on some bacterial pathogens incriminated in bovine Mastitis
118
123
EN
Ashraf
A.
Abdel-Tawab
Department of Bacteriology, Immunology and Mycology, Faculty of Veterinary Medicine, Benha University
Fatma
I.
El-Hofy
Department of Bacteriology, Immunology and Mycology, Faculty of Veterinary Medicine, Benha University
Ahmed
A.A.
Maarouf
Animal Health Research Institute, Benha Branch
Sameh
A.H.
Abbas
Kaluobia Vet. Med. Organization, El-
Kanater El-Khayria
10.21608/bvmj.2016.31356
In this study a total of 1200 quarter milk samples were selected from 300 cows from different localities<br />at El-Kaluobia governorate. The bacteriological examination revealed that a total of 125 isolates from<br />clinical and subclinical mastitic milk samples of which 57 S.aureus isolates were isolated from both<br />mastitic milk .with incidence of 45.6% of total isolates, 28 E.coli with 22.4%, 13 S.agalactia with 10.4%,<br />9 S. dysgalactia with 7.2%, 7 isolates of both S.uberis & coagulase negative staph (5.6%), 3 Klebsiella<br />sp with 2.4% and 1 Pseudomonas sp with 0.8%. Mean titer of anti S aureus specific IgA was higher in<br />milk of subclinical mastitic cases (1:845) than clinical one(1:290), while mean titer in milk of clinical<br />and subclinical mastitic cases much higher than in serum of clinical(1:54)and subclinical (1:41), while<br />mean titer of anti S. aureus specific IgM and IgG was much higher in serum of clinical(1:1382 , 1:868<br />for IgM&1:1267 , 1:634 for IgG ) than milk of clinical and subclinical mastitic cases (1:111 , 1: 53 for<br />IgM& 1: 302 , 1: 206 for IgG). Results of sensitivity tests of S.aureus against different antibiotic discs<br />revealed that 93%,91.2%,87.2% and 63.2%, were sensitive to Amoxicillin /Clavulanic acid,<br />Gentamycin, Cloxacillin, Sulphamethoxazole /Trimethoprim while87.7%,86.0%,84.2% and 82.5%of<br />isolates were resistant to Nalidixic acid, Streptomycin, Oxytetracycline and Penicillin-G.
mastitis,cattle,immunoglobulins,Antibacterial
https://bvmj.journals.ekb.eg/article_31356.html
https://bvmj.journals.ekb.eg/article_31356_6da663f40a8b4b51efdd0fd00687239f.pdf
Benha University; Faculty of Veterinary Medicine
Benha Veterinary Medical Journal
1110-6581
2974-4806
30
1
2016
03
01
Biochemical Studies on Evaluation of New Composite against Hepatocellular carcinoma-induced in Rats: Recent Therapeutic Approaches
124
136
EN
Omaima
Ragab
abou Zaid
Biochemistry Department, Faculty of Vet. Medicine, Benha University, Egypt
Yakout
A.
El- Senousy
Biochemistry Department, Faculty of Vet. Medicine, Benha University, Egypt
Abdel Fattah
M.
Badawy
Applied Chemistry Department, Egyptian Petroleum Research Institute, Egypt
Ahmed
Mohammed
Rashad
Biochemistry Department, Faculty of
Pharmacy, October 6 University, Egypt
10.21608/bvmj.2016.31357
The hepatoprotective effect of some natural and synthetic compounds against chemically induced<br />hepatocellular carcinoma in rats was evaluated. One hundred male albino rats were divided equally into<br />five groups. Normal control group, carcinogenic [Ferric nitrilotriacetate (Fe-NTA: 9 mg Fe/kg b.wt. i.p.)<br />and chloroform (150 mg/ kg b.wt. orally)] - induced group, curcumin group (400 mg/kg. b.wt. orally),<br />tetrachlorocuprate-lysine (25 mg/kg. b. wt. s.c.) and ascorbate (500 mg/kg. b. wt. orally) group and a<br />mixture group (composed of curcumin, tetrachlorocuprate -lysine and ascorbate). Blood samples and<br />liver tissue specimens were collected at the end of experiment (4 months) for determination of the<br />following parameters: Tumor necrosis factor-α (TNF-α), interleukin-1 (IL-1), catalase and<br />myeloperoxidase (MPO) in liver tissues, in addition to serum malondialdehyde (MDA) and reduced<br />glutathione (GSH). Moreover, histopathological examination of liver tissues was done for results<br />confirmation. The obtained results showed a significant elevation in MDA, MPO and immunological<br />markers levels, with significant reduction in serum reduced glutathione and catalase activity in liver<br />tissue in hepatocellular-carcinogen induced rats as compared to the control group. However,<br />administration of rats with the compounds under investigation resulted in a significant reduction of<br />MDA, MPO and immunological markers levels, and increased in reduced glutathione and catalase levels<br />compared to the carcinogenic non treated group. Various pathological alterations were observed in liver<br />of chemically induced-carcinogenic group interestingly, results supported the protective effect of the<br />compounds under investigation and preserved the histological structures of liver tissues. These results<br />concluded that basic curcumin, tetrachlorocuprate-lysine and ascorbate exert chemopreventative effect<br />against hepatocellular carcinoma.
Ferric nitrilotriacetate,Curcumin,antioxidant,hepatocellular carcinogen
https://bvmj.journals.ekb.eg/article_31357.html
https://bvmj.journals.ekb.eg/article_31357_a8e5d4f92c9887505c5c04782eeefe78.pdf
Benha University; Faculty of Veterinary Medicine
Benha Veterinary Medical Journal
1110-6581
2974-4806
30
1
2016
03
01
Molecular Screening Of Virulence Genes In Avian Pathogenic Esherichia Coli
137
149
EN
Ashraf
A.
Abd El Tawab
Bacteriology, Immunology and Mycology Dept. Fac. Vet. Med., Benha Univ.
Fatma
I.
El-Hofy
Bacteriology, Immunology and Mycology Dept. Fac. Vet. Med., Benha Univ.
Ahmed
M.
Ammar
Bacteriology, Immunology and Mycology Dept., Fac. Vet. Med. Zagazig Univ.
Noha
M.
Abdel Galil
Animal Health Research Institute (Zagazig branch)
10.21608/bvmj.2016.31358
Avian pathogenic Escherichia coli (APEC) causes colibacillosis, which is one of the main causes of<br />economic losses in the poultry industry worldwide. This disease occurs only when the E. coli infecting<br />strain presents virulence factors (encoded by specific genes) that enable the adhesion and proliferation<br />in the host organism. Herin, 15 E. coli strains of different serogroups isolated from birds with<br />colibacillosis were assigned to their phylogenetic groups and analyzed for the occurrence of 11<br />virulence associated genes. phylogenetic typing showed that group B2 was the largest (33.3%, 5/15)<br />followed by group B1 (26.7%, 4/15), groups A and D were similar in size (22.1%, 19/86). The virulence<br />profiles showed that ompA was found in most isolates 14/15 (93.3%). The iss gene was found in13/15<br />(86.6%). Followed by traT and iutA genes which were found in12/15 (80%). cvaC, stx2 and tsh genes<br />were present in 9/15(60%), 7/15(46%) and of the isolates, respectively. Only one isolate gave positive<br />amplification for stx1 and ibeA genes each. The hly gene was not encountered in any of the tested<br />isolates
Avian pathogenic Escherichia coli (APEC),Phylogenetic analysis,Virulence genes
https://bvmj.journals.ekb.eg/article_31358.html
https://bvmj.journals.ekb.eg/article_31358_808ac43e63f0b6237d9eaf0eb36bb777.pdf
Benha University; Faculty of Veterinary Medicine
Benha Veterinary Medical Journal
1110-6581
2974-4806
30
1
2016
03
01
Chemopreventive effect of omega- 3 fatty acids in N-nitrosodiethylamine Induced hepatocellular carcinoma in rats
150
160
EN
Samy
Hussein
Department of Biochemistry, Faculty of Vet. Med., Benha University, Egypt.
samyaziza@yahoo.com
Tahya
E.A.
Esmeal
Nutrition and clinical nutrition department, Faculty of Vet. Med., Benha University, Egypt
Aziza
Amin
Pathology Department, Faculty of Vet. Med., Benha University, Egypt
Eman
A.M.
Sarhan
Biochemistry Department, Faculty of Vet. Med., Benha University, Egypt
10.21608/bvmj.2016.31359
Liver cancer, predominantly hepatocellular carcinoma (HCC), represents a complex and fatal<br />malignancy driven primarily by oxidative stress and inflammation. The chemopreventive effect of<br />omega-3 on inflammatory markers and oxidative damage, caspase-3, antioxidant status and<br />histopathological alterations in hepatic tissue in N-nitrosodiethylamine (DEN)-induced HCC in male<br />rats was investigated. To induce HCC, rats were given DEN (200 mg/kg b.wt.,i.p) three times at a 15<br />day interval. 75 rats were divided into five equal groups. Control group, DEN group, DEN + ω3<br />protected group received omega-3 (270mg/kg b. wt/day) orally 4 weeks before DEN injection and<br />continued to 13 weeks, DEN + ω3 treated group firstly injected with DEN and orally treated with omega-<br />3 from the 8<sup>th</sup> week till the end of the experiment (13<sup>th</sup> week) and normal -ω3 group received omega-3.<br />Blood samples and liver tissues were collected at 13<sup>th</sup> week. DEN-induced HCC significantly decreased<br />SOD and CAT activities in liver tissue. However, a marked increase in liver tissue L-MDA, DNA<br />fragmentation, caspase-3 and Nf-kB P65 and in serum AFP, IL-6 and TNF-α levels were observed in<br />DEN injected rats. Initiation of HCC was demonstrated by histopathological examination of hepatic<br />tissue in DEN injected group. Histopathological examination confirmed the pathological improvement<br />in the liver of rats in omega -3 protected and treated groups. In conclusion, omega-3 demonstrates antiinflammatory property by suppression of TNF-alpha and IL-6 due to inhibition of NF-kB p65 and exerts<br />its chemopreventive effect on liver carcinogenesis. These data suggest that omega-3 exhibited<br />significant protection against DEN-induced HCC, which might be related with the enhancement of the<br />antioxidant activity and the induction of apoptosis.
Hepatocellular carcinoma,omega-3,Inflammatory mediators,oxidative damage,histopathology
https://bvmj.journals.ekb.eg/article_31359.html
https://bvmj.journals.ekb.eg/article_31359_d20063dccf4f44f9341cfcc4acc9a086.pdf
Benha University; Faculty of Veterinary Medicine
Benha Veterinary Medical Journal
1110-6581
2974-4806
30
1
2016
03
01
Molecular characterization of Aminoglycosoide and Tetracycline resistant Salmonella isolates causing new born ruminants diarrhea
161
170
EN
Ashraf
A.
Abd El- Twab
Department of Bacteriology, Immunology and Mycology, Faculty of Veterinary Medicine, Moshtohor, Benha University
Fatma
I.
El-Hofy
Department of Bacteriology, Immunology and Mycology, Faculty of Veterinary Medicine, Moshtohor, Benha University
Amira
Mohamed
Rizk
Department of Bacteriology, Immunology and Mycology, Faculty of Veterinary Medicine, Moshtohor, Benha University
amira.rizk01@fvtm.bu.edu.eg
10.21608/bvmj.2016.31360
Ten isolates of Salmonella species were obtained from sporadic cases of profuse diarrhea in new<br />born ruminants from El-Menofiya and El-Kalubia Governorates in Egypt. These isolates were as follow<br />7 isolates from calves (Salmonella Typhimurium, Salmonella Enteritidis,two were Salmonella<br />Saintpaul, two were Salmonella Langeveld and Salmonella Havana) ,two isolates from lambs<br />(Salmonella Typhimurium, Salmonella Bardo) and one isolate from goat-kids(Salmonella<br />Enteritidis). Salmonella isolates resistance to aminoglycosoides was 0.00% for Amikacin, 30% for<br />tobramycin and 50% for streptomycin. Susceptibility of isolates to tetracycline was 50% for<br />doxycycline. Results showed a high incidence of aminoglycosoides resistance gene aadB in 100% of<br />the isolates while aadA2 genes in 40% of the isolates. Incidence of tetracycline resistance genes was<br />0.00% for tetA(B) and 80% for tetA(A). The difference between the results of this study and those from<br />other regions in Egypt necessitate a complete survey overall the country to make a complete and clear<br />map of salmonella servoars, their antibiotics susceptibility and in molecular characterization of<br />resistance determinants in each region
diarrhea,newborn ruminants,Salmonella,aminoglycosoides,tetracycline
https://bvmj.journals.ekb.eg/article_31360.html
https://bvmj.journals.ekb.eg/article_31360_15ca6bb82ebe9967bcadaceeffe3dc58.pdf
Benha University; Faculty of Veterinary Medicine
Benha Veterinary Medical Journal
1110-6581
2974-4806
30
1
2016
03
01
Molecular characterization of Quinolones and β-Lactams Resistant Salmonella Serovars Determinants in Diarrheic Calves, lambs and goats-kids in the Middle of Nile Delta, Egypt
171
182
EN
Ashraf
A.
Abd El- Twab
Department of Bacteriology, Immunology and Mycology, Faculty of Veterinary Medicine, Moshtohor, Benha University
Fatma
I.
El-Hofy
Department of Bacteriology, Immunology and Mycology, Faculty of Veterinary Medicine, Moshtohor, Benha University
Amira
Mohamed
Rizk
Department of Bacteriology, Immunology and Mycology, Faculty of Veterinary Medicine, Moshtohor, Benha University
amira.rizk01@fvtm.bu.edu.eg
10.21608/bvmj.2016.31361
A total of (236 swabs) fecal samples from El-Menofiya and El-Kalubia Governorates, as sporadic cases<br />of were subjected to bacteriological, biochemical, serotyping, sensitivity testing and PCR detection of<br />resistance genes for β-Lactames and Quinolones. Ten isolates of Salmonella species were identified,<br />as 7 isolates (4.7%) from calves (Salmonella Typhimurium, Salmonella Enteritidis, Salmonella<br />saintpaul, Salmonella Langeveld and Salmonella Havana), 2 isolates (3.6%) from lambs (Salmonella<br />Typhimurium, Salmonella Bardo) and one isolate (3.3%) from goat-kids (Salmonella Enteritidis)<br />Salmonella isolates sensitivity to β-lacatmes was 0.00% for ampicillin, penicillin G, piperacillin,<br />cephalexin, cefoxitin, ampicillin-sulbactam, ceftazidime, 10% for ceftriaxone and 20% for amoxicillinclavulanic<br />acid, increased to 60% and 100% for aztreonam, imipenem respectively. Susceptibility of<br />isolates to quinolones were 10% for ciprofluxacin, 20% to nalidixic acid, and 100% for each of<br />norfloxacin and levofloxacin. PCR study showed beta-lactamase encoding gene, bla (TEM-1), was<br />identified in 90% and the extended-spectrum beta-lactamase, (CIT) in 20% while bla (SHV) and acc in<br />80% of isolates. The detection was 50%, 40%, and 0.00% for FOX, MOX, and bla (OXA-1) genes,<br />respectively. Plasmid-mediated quinolone resistance, qnrB, qnrS were detected in 80% while aac(6')-<br />Ib-cr, was only in 50% of the isolates. Results showed a high incidence of β-lacatmase than quinolones<br />resistance genes and higher isolate susceptibility to quinolone than to β-lacatmes which indicates higher<br />efficacy and validity of quinolones. Results indicates quinolones LEV and NOR in addition to β-<br />lacatmes imipenem are the drugs of choice for suspected salmonella cases.
diarrhea,calves,Salmonella,β-lacatmes,Quinolones
https://bvmj.journals.ekb.eg/article_31361.html
https://bvmj.journals.ekb.eg/article_31361_cb98442ae940c7956c0e782375e12029.pdf
Benha University; Faculty of Veterinary Medicine
Benha Veterinary Medical Journal
1110-6581
2974-4806
30
1
2016
03
01
Antimicrobial effect of some essential oils on Staphylococcus aureus in minced meat
183
191
EN
Hemmat
M.
Ibrahim
Department of food Hygiene, Fac. Vet. Med, Benha University
drhemmat01@yahoo.com
Rasha
A.
El Sabagh
Department of food Hygiene, Fac. Vet. Med, Benha University
Nahla
A.
Abou El-Roos
Food Hygiene Dept., Animal Health Research Institute Shebin El- Kom branch
Hend
Abd El Fattah
Department of food Hygiene, Fac. Vet. Med, Benha University
10.21608/bvmj.2016.31362
consumers increasingly demand of using natural preservatives as alternative to those chemical additives<br />that has been questioned in last years. So, the effect of some essential oils as thyme, clove and garlic as<br />antimicrobial agent against Staphylococcus aureus and their role in enhance shelf life of minced meat<br />were studied. The sensory analysis indicated significant advantages in using thyme, clove and garlic oils<br />in refrigerated minced beef. All used essential oils had considerable effectiveness in decreasing S. aureus<br />count. In addition, the results indicated that the bacterial counts decreased as the concentration of the oil<br />increased, accordingly, the concentration 1.5% of each oil gave the best effectiveness and the thyme oil<br />showed the highest action followed by clove and garlic oils.
Antimicrobial efficiency,Essential oils,Staphylococcus aureus,Minced meat
https://bvmj.journals.ekb.eg/article_31362.html
https://bvmj.journals.ekb.eg/article_31362_070189711533614399a0789cccd4fd05.pdf
Benha University; Faculty of Veterinary Medicine
Benha Veterinary Medical Journal
1110-6581
2974-4806
30
1
2016
03
01
The effects of dietary prebiotic on phagocytic activity of innate immunity in Oreochromis niloticus
192
198
EN
Ashraf
A.
Abd El Tawab
Bacteriology, Immunology and Mycology Department Faculty of Veterinary Medicine, Benha University
Fatma
Ibrahem
El-Hofy
Bacteriology, Immunology and Mycology Department Faculty of Veterinary Medicine, Benha University
Ahmed
M.
Amaar
Bacteriology, Immunology and Mycology Department Faculty of Veterinary Medicine,
Zagazig University
Osama
Abd El-Rahman
Saleh
Central Lab for Aquaculture Research, Egypt
Heba
Ahmed
Tolba
Central Lab for Aquaculture Research, Egypt
10.21608/bvmj.2016.31363
The objectives of this study, effects of prebiotic β polo on innate immunity of Oreochromis niloticus<br />(O.niloticus) and increase resist against disease. A total number of 180 Nile-tilapia fish (O. niloticus),<br />were divided into four equal groups; the first one was served as control fed on basal non-treated diet.<br />The second group fed on diet supplemented with 1.5 ml prebiotic βpolo /kg basal fish diet, the third<br />group fed on diet supplemented with 1 ml prebiotic βpolo/kg basal fish diet, and the fourth group fed on<br />diet supplemented with 0.5 ml prebiotic βpolo/kg basal fish diet for 40 days. The results showed that,<br />significant increase in phagocytic activity test and total leucocyte count (TLC) levels. The percent level<br />of protection among the three treated groups after challenge infection using Aeromonus sobria Aer.<br />sobria (0.5 ml of culture suspension of pathogen containing 107 bacteria ml-1) was higher than control<br />and the highest record was in the group received 1.5 ml prebiotic βpolo /kg basal fish diet. The results<br />of the present study support the use of 1.5 ml prebiotic βpolo /kg basal fish diet as fish<br />immunostimulants.
prebiotic,phagocytosis,Aeromonus sobria
https://bvmj.journals.ekb.eg/article_31363.html
https://bvmj.journals.ekb.eg/article_31363_cfc22d257e352819d50dac9ffdb7438d.pdf
Benha University; Faculty of Veterinary Medicine
Benha Veterinary Medical Journal
1110-6581
2974-4806
30
1
2016
03
01
Detection of Virulence factors of Pseudomonas species isolated from fresh water fish by PCR
199
207
EN
Ashraf
A.
Abd El Tawab
Bacteriology, Immunology and Mycology Dep., Fac. Vet. Med. Benha Univ.
Ahmed
A. A.
Maarouf
Animal Health Research "Benha branch"
Nesma
M.G.
Ahmed
Animal Health Research "Benha branch"
10.21608/bvmj.2016.31364
This study was conducted on 100 diseased Nile tilapia (O. niloticus) fish of various sizes collected from<br />different fish markets in Kaliobia Governorate to estimate the prevalence of Pseudomonas infection and<br />detection of some virulence genes in the isolated P. aeruginosa strains. The results of bacteriological<br />examination revealed that the prevalence of Pseudomonas septicemia with Pseudomonas species<br />isolation was 17.0% (17 \ 100 examined fish). These cases were attributed to P. anguilliseptica; P.<br />aeruginosa and P. fluorescens (14/43.7%; 12/37.5% and 6/18.8%), respectively. In addition, 32<br />Pseudomonas species were isolated, 11from liver samples (34.4%); 10 from kidney samples (31.2%); 6<br />from gill samples (18.8%) and 5 from skin samples (15.6%). Moreover, 14 P. anguilliseptica were<br />isolated with an incidence of 35.7%, 28.6%, 21.4% and 14.3% followed by 12 P. aeruginosa 33.3%,<br />25%,16.7% and 25% respectively; 6 P. fluorescens 33.3%; 50.0%,16.7% and 0.0% from the liver,<br />kidney, gill and skin samples respectively. The in-vitro antimicrobial sensitivity test showed that the<br />isolated Pseudomonas strains were sensitive to gentamycin; enrofloxacin; norfloxacin; ciprofloxacin<br />and florphenicol. Meanwhile; they were intermediate sensitive for doxycycline; sulfa-trimethoprim;<br />oxytetracycline; nalidixic acid and streptomycin. In contrast, they were resistant for cefotaxime;<br />erythromycin; amoxicillin; methicillin; oxacillin and ampicillin. Moreover, the PCR results revealed<br />that, opr L and exo S virulence genes were detected in all six studied strains (100.0%). Meanwhile, phz<br />M virulence gene was detected in 5 out of 6 studied strains (83.3%) and tox A virulence gene was<br />detected in 4 out of 6 studied strains (66.7%) i.e., all studied strains were Ps. aeruginosa and all of them<br />were virulent strains
fish,Bacteriological Evaluation,Pseudomonas Species,PCR,oprL,exoS genes
https://bvmj.journals.ekb.eg/article_31364.html
https://bvmj.journals.ekb.eg/article_31364_04f423b00188a75a6638d4c05572411a.pdf
Benha University; Faculty of Veterinary Medicine
Benha Veterinary Medical Journal
1110-6581
2974-4806
30
1
2016
03
01
Evaluation of protective and therapeutic role of zinc oxide nanoparticles and aloin on dextran sulfate-induced ulcerative colitis in rats
208
218
EN
Omayma
A.R.
AbouZaid
Biochemistry Department, Faculty of Vet. Med., Benha University, Egypt
Heba
M.
El-sogheer
Biochemistry Department, Faculty of Vet. Med., Benha University, Egypt
Sawsan
M.
El-sonbaty
Radiation Microbiology Dept., National Center for Radiation Research and Technology (NCRRT)
10.21608/bvmj.2016.31365
This study designed to investigate the anti –inflammatory effect of zinc oxide nanoparticles (ZnONPs)<br />and \ or aloe component (aloin) on inflammatory mediators and oxidative stress in dextran sulfate<br />sodium salt induced ulcerative colitis(UC) in rats. sixty-four albino rats divided into eight equal of eight<br />rats each. Group 1:(normal control) received no drugs , group 2:( ulcerative colitis) rats received dextran<br />sulfate sodium salt 3% in drinking water, group 3:( ZnONPs) rats administered ZnONPs (5mg/kg body<br />weight) orally for 3weeks, group 4: (aloin ) rats administered orally with 1ml of aloin 0.1% daily for<br />3weeks, group 5: (aloin + ZnONPs) rats orally administered with NZnO (5mg/kg body weight) and<br />(aloin 0.1%), daily for 3weeks, group 6: (ZnONPs +UC) rats with ulcerative colitis orally treated with<br />ZnONPs (5mg/kg body weight) daily for 3 weeks, group7: (aloin+UC) rats with ulcerative colitis orally<br />treated with (aloin 0.1%), daily for 3weeks, group 8: (aloin+ ZnONPs +UC) rats with ulcerative colitis<br />orally treated daily with ZnONPs (5mg/kg body weight)+aloin (1ml aloin 0.1%) for 3weeks. The<br />obtained results revealed that, administration of ZnONPs and\or aloin to rats with ulcerative colitis<br />significantly reduced elevated serum total cholesterol and TG concentrations, and markedly increased<br />the reduced HDL-C level. On the other hand, elevated level of COX-2, IL-6, MDA and TNF-α in UC<br />rats were significantly reduced, with significant increase of the reduced level of GSH. Results suggest<br />that ZnONPs modulates UC, while aloin showed high efficacy to normalize UC tissues and may<br />considered as potential treatment for UC and other inflammatory bowel disease.
Zinc Oxide Nanoparticles,aloin,ulcerative colitis,Inflammatory mediators
https://bvmj.journals.ekb.eg/article_31365.html
https://bvmj.journals.ekb.eg/article_31365_b8743342d47383695c2b5fb77df8ace9.pdf
Benha University; Faculty of Veterinary Medicine
Benha Veterinary Medical Journal
1110-6581
2974-4806
30
1
2016
03
01
Molecular characterization for some virulence and antibiotic resistance genes of Staphylococcus aureus isolated from dairy cattle's subclinical mastitis in EL- Sharkia Governorate
219
230
EN
Ashraf
A.
Abd El-Tawab
Bacteriology, Immunology and Mycology Dep., Fac. of Vet. Med., Benha Univ.
atma
I.
El Hofy
Bacteriology, Immunology and Mycology Dep., Fac. of Vet. Med., Benha Univ.
Ahmad
M.
Amaar
Bacteriology, Immunology and Mycology Dep., Fac. of Vet. Med., Zagazig Univ.
Mokhtar
Abd El-Hakeem
Sleim
Animal Health Research Institute (Zagazig branch)
Heba
S. S.
Salem
Animal Health Research Institute (Zagazig branch)
10.21608/bvmj.2016.31366
Staphylococcus aureus has emerged as a significant public health problem as it is often responsible for<br />intramammary infection in bovine. The emergence of MRSA in animals was from an outbreak of<br />mastitis in cattle which represent a great economic in milk industry. The present study was carried<br />out to genotypically characterized S. aureus isolated from subclinical bovine mastitis in different farms<br />in EL- Sharkia Governorate. A total of 15 S. aureus isolates were obtained from 100 subclinical mastitic<br />milk samples and subjected to PCR for detection of some virulence and antimicrobial resistance genes<br />using oligonucleotide primers that amplified genes encoding enterotoxin genes A to E (sea, seb, sec,<br />sed, see), coagulase gene (coa), the IgG binding region of protein A (spa) and resistance gene as factor<br />essential for expression of methicillin resistance (femA) which was used as an internal positive control<br />and intrinsic methicillin resistance gene (mecA). PCR amplification revealed that all S. aureus isolates<br />were enterotoxogenic and MRSA, harbored the genes encoding staphylococcal coagulase and the genes<br />encoding the immunoglobulin G binding region of protein A. The data in the study provided an overview<br />on the distribution of virulence determinants of MRSA strains which contributed to bovine mastitis<br />problem in the Egypt farm.
Staphylococcus aureus,Polymorphism,Genotyping,Virulence factors,mastitis
https://bvmj.journals.ekb.eg/article_31366.html
https://bvmj.journals.ekb.eg/article_31366_1e7446037bfcb6c237bf3dfe67d63204.pdf
Benha University; Faculty of Veterinary Medicine
Benha Veterinary Medical Journal
1110-6581
2974-4806
30
1
2016
03
01
A study of outer membrane protein (OMPs) genes for detection of salmonella organisms in poultry farms
231
237
EN
Ashraf
A.
Abd El Tawab
Bacteriology, Immunology and Mycology Dep., Fac. Vet. Med. Banha Univ.
Mai
M.
Elsayed
Bacteriology, Immunology and Mycology Dep., Fac. Vet. Med. Banha Univ.
Ahmed
M.
Amaar
Microbiology Dep., Fac. Vet. Med, Zagazig Univ.
Amany
I.
El-bialy
Serology Dep., animal health institute, Dokki
10.21608/bvmj.2016.31367
Bacteriological examination of internal organs obtained from diseased layers, broilers and baby chicks<br />revealed isolation of salmonellae with an incidence 5%, 2.5% and 7.1%, respectively. Eleven<br />Salmonella isolates were recovered out of 250 poultry samples. The highest percentage rate was in liver<br />4%, followed by intestine 2.4% and finally spleen 0.8%. Salmonella organisms were recovered from<br />ovary of layers with an incidence 3.33%. Serological identification of Salmonella isolates revealed that<br />the most prevalent serovars was. Enteritidis (3) followed by. Gallinarum (2) and Rissen (2) then S.<br />Kentukey, S. Florida, S. Lomita and S. Sontheim (one isolate for each). The goal of this study was to<br />evaluate the suitability of the outer membrane genes OmpA and OmpF for detection of Genus<br />Salmonella in clinical samples. OmpA gene was detected in 100% of the examined clinical samples<br />while OmpF gene was detected in 14 clinical samples. Three samples were positive by OmpF.PCR more<br />than cultural method. It was concluded that PCR protocol decrease the time needed for the detection of<br />Salmonella OmpA gene couldn't discriminate genus Salmonella from other non-Salmonella organisms<br />in clinical samples .AS it amplified OmpA in other Enterobacteriaceae. However, OmpF gene is<br />promising tool for detection of Genus Salmonella
S. enteritidis,outer membrane protein genes,PCR
https://bvmj.journals.ekb.eg/article_31367.html
https://bvmj.journals.ekb.eg/article_31367_458bf850423c0bf3b107207d763bf66d.pdf
Benha University; Faculty of Veterinary Medicine
Benha Veterinary Medical Journal
1110-6581
2974-4806
30
1
2016
03
01
Molecular detection of some virulence genes of S. aureus isolated from mastitic Cows by PCR
238
245
EN
Ashraf
A.
Abdel-Tawab
Department of Bacteriology, Immunology and Mycology, Faculty of Veterinary Medicine, Benha University
Fatma
I.
El-Hofy
Department of Bacteriology, Immunology and Mycology, Faculty of Veterinary Medicine, Benha University
Ahmed
A.A.
Maarouf
Animal Health Research Institute, Benha Branch
Sameh
A.H.
Abbas
Kaluobia Vet. Med. Organization, El-Kanater El-Khayria
10.21608/bvmj.2016.31368
In this study a total of 1200 quarter milk samples were selected from 300 cows at different localities of<br />El-Kaliobia Governorate were examined bacteriologically and revealed that 57 S.aureus isolated from<br />both clinical and subclinical mastitis with incidence of 45.6% of total isolates. Molecular typing of S.<br />aureus by PCR revealed that all isolates (100%) (12 random S.aureus isolates) were positive for the 16<br />SrRNA genes of S. aureus. Genotyping of virulence genes encoding factors of S. aureus revealed that<br />two isolates were positive for (tst-1) gene (16.7%), three isolates positive for (etb) 25%, nine isolates<br />positive for each of (icaD and femA) genes (75%), seven isolates positive for (hlg) (58.3%), eight isolates<br />positive for (nuc) (66.7%).
mastitis,S. aureus,PCR,Virulence genes
https://bvmj.journals.ekb.eg/article_31368.html
https://bvmj.journals.ekb.eg/article_31368_cbcf41c26faa0e1a31cc2816faa0de3a.pdf
Benha University; Faculty of Veterinary Medicine
Benha Veterinary Medical Journal
1110-6581
2974-4806
30
1
2016
03
01
Rapid tests for detection of ciprofloxacin residues in liquid milk
246
253
EN
Hamdi
A.
Mohammed
Department of Food Control, Faculty of Veterinary Medicine, Benha University
Adham
M.
Abdou
Animal Health Research Institute, Tanta Branch
Amal
M.
Eid
Animal Health Research Institute, Tanta Branch
amalaeidvet@gmail.com
Asem
M.
Zakaria
Animal Health Research Institute, Tanta Branch
10.21608/bvmj.2016.31369
A total of 234 randomly selected milk samples from different sources (110, 50, 34 and 40 samples) from<br />each of (market milk, collecting centers, bulk tank and UHT milk samples; respectively) were analyzed<br />for the quinolones residues by rapid quinolones detection strip test. Results revealed that 17.3%, 14%<br />and 20.6% of milk samples were positive for the presence of quinolones residues for market milk,<br />collecting centers and bulk tank milk samples; respectively, while not detected in UHT milk samples.<br />The positive milk samples were subjected to HPLC analysis for quantitative detection of ciprofloxacin<br />residues, which were present in 11.8%, 8%, and 8.8% for market milk, collecting centers and bulk tank<br />milk samples; respectively, with mean values of 17.37± 0.27, 18.02±0.7 and 17.79± 0.75 ppb;<br />respectively. None of them had a concentration exceeded the maximum residual limit set by different<br />international standards. Public health importance of ciprofloxacin residues was discussed
Rapid Detection,ciprofloxacin,Liquid Milk
https://bvmj.journals.ekb.eg/article_31369.html
https://bvmj.journals.ekb.eg/article_31369_e860e1b6709f01fbcd4d2e02d63b2f4e.pdf
Benha University; Faculty of Veterinary Medicine
Benha Veterinary Medical Journal
1110-6581
2974-4806
30
1
2016
03
01
Persistence of deltamethrin and diazinon in environment of dairy farm
254
259
EN
Marzouk
M.
A.
Department of Animal, Poultry and Environmental Hygiene, Faculty of Veterinary Medicine, Benha University
Ashoub
M.
M.A.
Department of Animal, Poultry and Environmental Hygiene, Faculty of Veterinary Medicine, Benha University
Metawea
Y.
F.
Department of Animal, Poultry and Environmental Hygiene, Faculty of Veterinary Medicine, Benha University
Azam
A.E.
S.
Department of Animal, Poultry and Environmental Hygiene, Faculty of Veterinary Medicine, Benha University
Mansour
A.
H.
Department of Food Hygiene in Biotechnology, Animal Health Research Institute, Dokki, Cairo, Egypt
10.21608/bvmj.2016.31370
The present study was carried out to investigate the persistence of some pesticides (diazinon and<br />deltamethrin) in water, feeds and milk. The obtained results illustrated that diazinonwas persisted for<br />longer time in water, feed and milk samples than deltamethrin, it disappears within 21<sup>st</sup> and14<sup>th</sup> day after<br />application in water and feed, respectively, while it disappears within 30<sup>th</sup> day after application in milk.<br />Moreover, deltamethrin disappear within 7<sup>th</sup> day after application in feed and water, while in milk it<br />disappears within 21<sup>st</sup> day after application. We concluded that diazinon and deltamethrin were persisted<br />in milk for longer time than both feed and water. Pesticides contamination of milk and milk products<br />may lead to acute or chronic toxicity for human being representing public health hazards through their<br />cumulative effects
Diazinon,Deltamethrin,persistence,Milk
https://bvmj.journals.ekb.eg/article_31370.html
https://bvmj.journals.ekb.eg/article_31370_95cd7895669d545f14299a9902793475.pdf
Benha University; Faculty of Veterinary Medicine
Benha Veterinary Medical Journal
1110-6581
2974-4806
30
1
2016
03
01
Biochemical effects of ear infections by Pseudomonas aeruginosa on rabbits
260
265
EN
Mohamed
K.
Mahfouz
Department of Biochemistry, Faculty of Veterinary Medicine, Benha University
Mahmoud
M.
Youseif
Department of Biochemistry, Faculty of Veterinary Medicine, Benha University
Abdel-Baset
I.
El-Mashad
Department of Pathology, Faculty of Veterinary Medicine, Benha University
Mohamed
Shendy
Department of ENT, Faculty of Medicine, Benha University
10.21608/bvmj.2016.31371
Ear infections (particularly, otitis media) is a prevailing and common infection in developing countries<br />causing local damage and threatening complications. Pseudomonas aeruginosa is the most common<br />pathogen causing chronic suppurative otitis media (CSOM) and malignant otitis externa. The objective<br />of this study is to identify incidence of Pseudomonas aeruginosa involved in ear infections and<br />associated biochemical parameters which may be changed. External auditory canal in rabbit ears was<br />inoculated with (10<sup>6</sup>) colony-forming units (CFU) of Pseudomonas aeruginosa or left as sterile controls<br />for eight weeks. There are significantly decrease results at (P<0.05) of Interleukin-2 and significant<br />increase results of Interleukin-9 and Tumor Necrosis Factor-alpha (TNF-α) in acute or chronic<br />infections. Moreover, histopathological changes of ear, kidney and liver tissues were also observed.<br />Evaluation of these parameters were needed to use of specific blocking agents to inflammatory<br />mediators with the aim of discovering new treatment options for chronic otitis media, and to help the<br />physicians in diagnosis these diseases in elderly stages and try to reduce the pain and suffering associated<br />with otitis media.
Otitis Media (OM),Pseudomonas aeruginosa,Interleukins
https://bvmj.journals.ekb.eg/article_31371.html
https://bvmj.journals.ekb.eg/article_31371_2f500b5a806604eca943df6a3eb13079.pdf
Benha University; Faculty of Veterinary Medicine
Benha Veterinary Medical Journal
1110-6581
2974-4806
30
1
2016
03
01
Some teratological effects of difloxacin in rats
266
271
EN
Ashraf
Elkomy
Department of Pharmacology, Faculty of Veterinary Medicine, Benha University
Mohamed
Aboubakr
Department of Pharmacology, Faculty of Veterinary Medicine, Benha University
Nermin
Medhat
Department of Pharmacology, Faculty of Veterinary Medicine, Benha University
10.21608/bvmj.2016.31373
The aim of this experiment was to detect the teratogenic effects of difloxacin in Wister Albino rats.<br />Thirty pregnant rats were divided into 3 groups; group (1) behaved as control and received normal saline.<br />Group (2) was administered difloxacin at a dose of 20 mg/kg b. wt and group (3) was administered<br />difloxacin at a dose of 40 mg/kg b. wt orally once daily from 6<sup>th</sup> to 15<sup>th</sup> day of pregnancy. The study<br />revealed no teratogenic effect at a dose of 20 mg/kg b. wt, while at a dose of 40 mg/kg b.wt has<br />significant teratogenic effect showed by decrease in size, weight, length, retarded growth in fetuses, fetal<br />resorption of treated groups, the incidences of skeletal & visceral abnormalities were increased in treated<br />group (40 mg/kg b. wt). It could be concluded that. Administration of difloxacin during early stage of<br />pregnancy and at high dose induce some fetal abnormalities.
difloxacin,Pregnancy,Visceral and Skeletal Abnormalities
https://bvmj.journals.ekb.eg/article_31373.html
https://bvmj.journals.ekb.eg/article_31373_df09f94525486172ee48e51c709f80b5.pdf
Benha University; Faculty of Veterinary Medicine
Benha Veterinary Medical Journal
1110-6581
2974-4806
30
1
2016
03
01
Biochemical effects of intestinal surgery in gastrointestinal enzyme
272
282
EN
Hussein
A.
Ali
Department of Biochemistry, Faculty of Veterinary Medicine, Benha University
hussein.ali@fvtm.bu.edu.eg
Mohamed
K.
Mahfouz
Department of Biochemistry, Faculty of Veterinary Medicine, Benha University
Badran
M.
Abd-elwahab
Department of Biochemistry, Faculty of Veterinary Medicine, Benha University
Adel
Sayed
Ahmed
Department of Surgery, Faculty of Veterinary Medicine, Benha University
10.21608/bvmj.2016.31376
This study was performed to investigate the effected of experimental work of intestinal surgery for<br />removal caecum of rabbits on alanine amiotransferase (ALT), aspartate aminotransferase(AST), gamma<br />glutamyletransferase (GGT), alkaline phosphatase (ALP), acid phosphotase (ACP), amylase, lipase,<br />catalase (CAT), ceratine phosphokinase (CPK), lactate dehydrogenase (LDH) and cortisol hormone.<br />Two groups of thirteen healthy female New Zland rabbits were used. The first one contained 15 rabbits<br />and kept as control group. The second contain also 15 rabbits subjected to surgery for removal of caecum<br />of rabbits. Blood sample were collected at 3, 6 and 9 days post-operative. The obtained result showed<br />that the increase in serum ALT, AST, GGT, ALP, ACP, amylase, lipase, CAT, CPK, LDH and cortisol<br />hormone were significant increase in experimental group compared with control group. From this result<br />it could be concluded that the biochemical parameter affected with intestinal surgery for removal of<br />rabbit’s caecum
Rabbits,Caecum,Surgery
https://bvmj.journals.ekb.eg/article_31376.html
https://bvmj.journals.ekb.eg/article_31376_33de20dc66eb5454472ffaaa432063df.pdf
Benha University; Faculty of Veterinary Medicine
Benha Veterinary Medical Journal
1110-6581
2974-4806
30
1
2016
03
01
Dual effect of Aspergillus Fumigatus and E. coli O125 in broilers
283
294
EN
Ibrahiem
M.
El-Boroaay
Department of Poultry Diseases, Faculty of Veterinary Medicine, Benha University
Samer
M.
Abd El Kader
Department of Poultry Diseases, Faculty of Veterinary Medicine, Benha University
Adel
A.
Shaheen
Department of Fish Diseases and management, faculty of Veterinary Medicine, Benha University
10.21608/bvmj.2016.31377
This study was conducted on 88 broiler chicks from one day to five days. They were collected from<br />three hatcheries at two governorates to detect the prevalence of infection by Aspergillus spp and/or E.<br />coli serotypes. This study revealed that the prevalence was 35.2%,43.2% and 18.2%for E. coli<br />serotypes, Aspergillus spp and both infection together, respectively. The clinical signs of the examined<br />broiler chicks appeared as weakness, respiratory manifestation (gasping) and diarrhea. The postmortem<br />lesions were mainly on lungs and air sacs which appeared congestion, white nodules in lungs and<br />yellowish white cheesy materials on air sacs and lungs. Some chicks showed signs of enteritis.<br />Bacteriological and mycological examinations for collected broiler chicks revealed that E. coli O125<br />and Aspergillus fumigatus were the most common isolates. Experimental infection of 124 one-day old<br />broiler chicks which checked and confirmed to be free from both infectious agents with inoculation of<br />E. coli O125 (intranasal/ one dose) and/or Aspergillus fumigatus (intra air sacs/one dose) showed<br />weakness, respiratory manifestation(gasping), diarrhea, congested lungs and cheesy materials on lungs<br />and air sacs. Reisolation and identification revealed positive results for infectious agents.<br />Histopathological examination was also described. Concerning to growth performance and mortality<br />rate, dual infection showed much decrease in weight and increase in mortality rate than single infection.<br />In conclusion the dual infection has more pathogenic effect than single infection.
Aspergillus fumigatus,E. coli,Broilers,intranasal and intra air sacs inoculation
https://bvmj.journals.ekb.eg/article_31377.html
https://bvmj.journals.ekb.eg/article_31377_e162fb3f7fcccef98557e0a85fbcfe1b.pdf
Benha University; Faculty of Veterinary Medicine
Benha Veterinary Medical Journal
1110-6581
2974-4806
30
1
2016
03
01
Antifungal Effect of Some Chemical Preservatives on Aspergillus Niger in Minced Beef Meat
295
301
EN
Amani
M.
Salem
Food Control Dept., Fac. Vet. Med., Benha Univ.
amani.salem@fvtm.bu.edu.eg
Reham
A.
Amin
Food Control Dept., Fac. Vet. Med., Benha Univ.
rehamnour2007@yahoo.com
Dalia
F.
Khater
Food Hygiene, Animal Health Research Institute, Tanta Branch
Loaloa
A.
Shokr
0000-0002-6040-8265
Food Hygiene, Animal Health Research Institute, Kafr El-Sheikh Branch
loaloa.shokr@gmail.com
10.21608/bvmj.2016.31399
A grand total of 1400 g fresh minced beef meat samples were divided into 7 equal groups (200 g of<br />each) and then irradiated. Aspergillus niger was inoculated into each group with infective dose 105<br />cfu/g. The used chemical preservatives natamycin (100, 200 and 300 ppm) and potassium sorbate (0.1%,<br />0.2% and 0.3%) were added. The inoculated samples were stored at 4oC in refrigerator till be used. The<br />inoculated groups were examined every 24 hrs for A. niger count and sensory examination (overall<br />acceptability). The experiment was performed in triplicate. Natamycin (100, 200 and 300 ppm)<br />decreased count of A. niger (log cfu/g) from 5.00 (initial load) to 2.83, 2.79 and 2.76 with reduction<br />percentages 43.40%, 44.20% and 44.80% on 8th, 8th and 10th days of storage, respectively. Potassium<br />sorbate (0.1%, 0.2% and 0.3%) decreased count of A. niger (log cfu/g) from 5.00 (initial load) to 3.02,<br />2.97 and 2.85 with reduction percentages 39.60%, 40.60% and 43.00% on 8th, 8th and 10th day of<br />storage, respectively. While, in control group A.niger count increased From 5.00 (initial load) to 5.56<br />on 6th day Furthermore, natamycin (300 ppm) treated minced meat samples artificially inoculated with<br />A. niger showed overall acceptability till 8th day of storage. In comparison, potassium sorbate (0.3%)<br />showed overall acceptability till 7th day. While, control group showed overall acceptability till 4th day.<br />Generally, natamycin (300 ppm) proved to be more efficient than potassium sorbate (0.3%) in<br />suppression of A. niger growth in minced meat. So, the use of natamycin (300 ppm), as it is safe<br />antifungal agent, is therefore recommended to improve safety of the meat products.
Aspergillus niger,antifungal effect,meat products,food preservative
https://bvmj.journals.ekb.eg/article_31399.html
https://bvmj.journals.ekb.eg/article_31399_f76e043ba29772a9071f95c0311b5d79.pdf
Benha University; Faculty of Veterinary Medicine
Benha Veterinary Medical Journal
1110-6581
2974-4806
30
1
2016
03
01
Efficacy of a locally prepared bovine mastitis vaccine
302
311
EN
Sayed
M.
L.
Central Laboratory for Evaluation of Veterinary Biologics
Shell
W.
S.
Central Laboratory for Evaluation of Veterinary Biologics
Hanan
A.
Ahmed
Central Laboratory for Evaluation of Veterinary Biologics
Hanan
M.
Ibrahim
Central Laboratory for Evaluation of Veterinary Biologics
Ali
A.
M.
Central Laboratory for Evaluation of Veterinary Biologics
Nasr
E.
A.
Veterinary Serum and Vaccine Research
Institute, Abassia, Cairo, Egypt
10.21608/bvmj.2016.31400
The objective of the present work is to prepare and evaluate the effectiveness of a locally prepared<br />polyvalent vaccine against mastitis from the most common causes of mastitis. Staphylococcus aureus,<br />Streptococcus agalactiae and Escherichia coli were the most prevalent bacteria recovered from clinical<br />and subclinical mastitis. MontanideISA-206 adjuvanted inactivated polyvalent vaccine containing the<br />three strains was prepared. Twenty pregnant cows were inoculated intramuscularly with the prepared<br />polyvalent vaccine two months prior to calving and boostering at day 21 from the primary injection.<br />Serum samples from vaccinated and non-vaccinated cows were collected at the 1<sup>st</sup>, 3<sup>rd</sup>, 8<sup>th</sup>, 12<sup>th</sup>, 16<sup>th</sup>, 20<sup>th</sup><br />and 24<sup>th</sup> weeks post vaccination and evaluated immunologically using ELISA. The results showed that<br />immune response was significantly higher in the vaccinated group than that of controls. These results<br />could be indicated the safety and effectiveness of the vaccine in reduction of incidence and severity of<br />clinical cases of mastitis but further studies should be done to elucidate the possibility of field application<br />and effectively
mastitis,vaccine,S. aureus,S. agalactiae,E. coli
https://bvmj.journals.ekb.eg/article_31400.html
https://bvmj.journals.ekb.eg/article_31400_71772ca9e045e4f37e062f93ad606edc.pdf
Benha University; Faculty of Veterinary Medicine
Benha Veterinary Medical Journal
1110-6581
2974-4806
30
1
2016
03
01
Seasonal variations on the levels of some pesticide residues in dairy farms
312
322
EN
Marzouk
M.
A.
Department of Animal, Poultry and Environmental Hygiene, Faculty of Vet. Med., Benha University
Mona
M.A.
Ashoub
Department of Animal, Poultry and Environmental Hygiene, Faculty of Vet. Med., Benha University
mona.aashoub@fvtm.bu.edu.eg
Metawea
Y.
F.
Department of Animal, Poultry and Environmental Hygiene, Faculty of Vet. Med., Benha University
Aya
E.S.
Azam
Department of Animal, Poultry and Environmental Hygiene, Faculty of Vet. Med., Benha University
Mansour
A.
H.
Department of food hygiene in biotechnology at Animal Health Research Institute, Dokki
10.21608/bvmj.2016.31401
The present work was carried out to study the effect of seasonal variations on the levels of pesticide<br />residues in water, feeds and milk. Our results illustrated that the significantly highest concentrations of<br />OCs in water and feed were detected during summer [(1.05±0.35, 0.72±0.24, 0.77±0.25, 2.5±0.70 and<br />0.81±0.27 μg/L, for p.p.DDT, aldrin, β-BHC, lindane and γ.chlordane respectively in water samples)<br />and (30.0±10.20, 45.00±13.02, 65.33±21.90, 35.33±13.20, 43.67±13.30, 22.00±7.33 and 19.13±8.6 μg<br />/kg, for p.p.DDD, p.p.DDE, p.p.DDT, aldrin, lindane, γ.chlordane and methoxychlor, respectively in<br />feed samples) ], while the lowest were detected during spring, on the other hand, none of pesticides<br />detected in winter which may be attributed to environmental factors. The significantly highest<br />concentration of diazinon in water and feed was recorded during autumn (78.75±26.30 μg/L and<br />140.00±64.60 μg/kg, respectively), while the lowest levels were detected during spring. Also, the<br />highest concentrations of deltamethrin was detected during spring in water (55.30±18.50 μg/L) and<br />during summer in feed samples (78.40±26.70 μg/kg), while the lowest levels were detected during<br />autumn in water and during spring in feed samples. The significantly highest concentrations of residues<br />in milk were detected during summer (p.p.DDD, p.p. DDE, p.p.DDT, heptachlor, heptachlor epoxide,<br />γ.chlordane, methoxychlor, diazinon and deltamethrin were 20.10±6.01, 52.30±17.20, 33.20±20.06,<br />1.16±0.38, 6.72±0.90, 2.570±2.23, 0.70±0.20, 58.50±19.20 and 45.13±15.10 μg/kg, respectively) and<br />the lowest were detected in winter. Our results concluded that the seasonal variations greatly influence<br />the levels of all examined pesticide residues with variable degree specially diazinon and deltamethrin.
Seasonal variations,OCs,Diazinon,Deltamethrin,Milk,water,feed
https://bvmj.journals.ekb.eg/article_31401.html
https://bvmj.journals.ekb.eg/article_31401_1a63cdca4c8cb786f8aa122b32682f5d.pdf
Benha University; Faculty of Veterinary Medicine
Benha Veterinary Medical Journal
1110-6581
2974-4806
30
1
2016
03
01
Detection of some foodborne pathogens in meat products by Polymerase Chain Reaction
323
330
EN
George
A.
Armany
Genome Research Unit., Animal Health Research Institute, Dokki
Hanaa
A.
Ahmed
Genome Research Unit., Animal Health Research Institute, Dokki
Hemmat
M.
Ibrahim
Food hygiene Dept., Faculty of Veterinary Medicine, Benha University
drhemmat01@yahoo.com
Reham
A.
Amin
Food hygiene Dept., Faculty of Veterinary Medicine, Benha University
rehamnour2007@yahoo.com
10.21608/bvmj.2016.31402
A total of 100 random samples of meat products including raw minced meat, raw sausage, luncheon and<br />basterma (25 samples of each) were collected from different markets in Cairo and Giza governorates to<br />be examined bacteriologically for detection of Staphylococcus aureus, Listeria monocytogenes and<br />Escherichia coli. These samples were examined for isolation of such pathogens by conventional<br />bacteriological methods and by polymerase chain reaction (PCR). Concerning S.aureus bacteriological<br />results revealed the prevalence in minced meat, Sausage, luncheon and basterma was (24%, 24%, 20%,<br />4%) respectively. While L. monocytogenes revealed the prevalence in minced meat, sausage, luncheon<br />and basterma was (4%, 0%, 0%, 0%) respectively and E. coli revealed the prevalence in minced meat,<br />sausage, luncheon and basterma was (20%, 20%, 24%, 20%) respectively. The results cleared that PCR<br />is an ideal method for identification of foodborne pathogens, as it was effective, less labor, more<br />sensitive, reduces effort and time after using gradient PCR in validation of each microbe.
Foodborne pathogens,Optimization,polymerase chain reaction (PCR),bacteriological isolation,meat products
https://bvmj.journals.ekb.eg/article_31402.html
https://bvmj.journals.ekb.eg/article_31402_4127b5ea6c63e2bf769f2324f13b8421.pdf
Benha University; Faculty of Veterinary Medicine
Benha Veterinary Medical Journal
1110-6581
2974-4806
30
1
2016
03
01
Effects of Sunflower Meals Inclusion on Performance and Some Haematological Parameters in Broiler chicks
331
340
EN
Nasser
E.
Khedr
Department of Nutrition and Clinical Nutrition, Faculty of Veterinary Medicine, Benha University
Tahia
E.
Ahmed
Department of Nutrition and Clinical Nutrition, Faculty of Veterinary Medicine, Benha University
Fatma
Ragab
Department of Nutrition and Clinical Nutrition, Faculty of Veterinary Medicine, Benha UniversityFatma Ragab
10.21608/bvmj.2016.31403
A total of two hundred twenty five one-day-old Indian river broiler chicks (IR) were distributed into<br />five different treatment groups (A, B, C, D and E), of three replicates (15 chicks / replicate). The chicks<br />in control group (A) were fed control diet with β- mannanase (Hemicell<sup>®</sup>), groups B and C were fed<br />diets containing SFM (2.5 and 5%, respectively) without β-mannanase (Hemicell<sup>®</sup>). While groups D<br />and E were fed diets containing SFM (2.5 and 5%, respectively) with β-mannanase (Hemicell<sup>®</sup>). Effects<br />of inclusion Sunflower meals with or without β-mannanase (Hemicell<sup>®</sup>) on performance showed that<br />groups containing Sunflower meals 2.5%with or without enzyme show improvements than other groups.<br />Erythrogram profile showed a significant (p≤0.05) difference between control group and other groups<br />in Hb, RBCs, PCV and MCV levels, while there was a significant (p≤0.05) increase in MCH and MCHC<br />in group E (SFM 5%+Hemicell<sup>®</sup>) when compared with other groups. Leukogram showed nonsignificant<br />changes between control group and other groups. It was concluded that inclusion of<br />sunflower meals 2.5% had a positive role in improvement final body weight changes and final body<br />weight gain. Partial replacement diet with sunflower meals (2.5 and 5% with or without Hemicell<sup>®</sup>) did<br />not induce side effects on hematological parameters
broiler chicks,Sunflower meals,β-mannanase (Hemicell®),Chick performance and hematological profile
https://bvmj.journals.ekb.eg/article_31403.html
https://bvmj.journals.ekb.eg/article_31403_92af1b7841d53af30423bbec752f6131.pdf
Benha University; Faculty of Veterinary Medicine
Benha Veterinary Medical Journal
1110-6581
2974-4806
30
1
2016
03
01
Effect of some factors on prevalence of FMDV antigen in both cattle and buffaloes in Egypt
341
347
EN
Hassan
A.
Aidaros
Department of Animal, Poultry and Environmental Hygiene, Faculty of Vet. Med., Benha University
Mona
M.A.
Ashoub
Department of Animal, Poultry and Environmental Hygiene, Faculty of Vet. Med., Benha University
mona.aashoub@fvtm.bu.edu.eg
Hala
G. A.
El Daous
Department of Animal, Poultry and Environmental Hygiene, Faculty of Vet. Med., Benha University
Hiam
M.
Fakhry
Department of foot and mouth disease at Veterinary Serum and Vaccine Research Institute, Abbassia, Cairo
10.21608/bvmj.2016.31404
The present study was carried out for detection and identification of the isolated FMDV from infected<br />tissue samples using serotyping antigen detection ELISA kit (IZSLER ELISA Kit).Thirty six lesion<br />samples were collected from different localities (Menofia, Gharbia, Kalyoubia, Giza and Sharquia<br />governorates) during the period from May 2014 to July 2015.The obtained results indicated that<br />clinically, unvaccinated infected cattle in Gharbia governorate 2014were positive to FMDV infection<br />serotype O. Samples collected from infected, vaccinated cattle and buffaloes in Kalyoubia governorate<br />during 2015 were positive to FMDV infection serotype O. Clinically infected cattle and buffaloes in<br />Menofia governorate during 2015 seven samples were positive to FMDV infection, which serotyped as<br />five samples were serotype O & two samples were SAT-2. Clinically infected cattle and buffaloes in<br />Giza governorate during 2015 sixteen samples were positive to FMDV infection, which eight samples<br />from them serotyped as SAT-2 & seven samples from them were serotype O & one of them was<br />serotyped A. Clinically infected un vaccinated cattle in Sharquia governorate 2015 three samples were<br />positive to FMDV infection serotype A. We concluded that large number of both vaccinated and un<br />vaccinated animals in these governorates affected by FMDV infection by its different serotypes in the<br />period of the study, and there are different factors affecting prevalence of FMDV as (age, state of<br />vaccination, type of vaccination, sex, season and species).
FMD,Antigen detection ELISA,tongue epithelium
https://bvmj.journals.ekb.eg/article_31404.html
https://bvmj.journals.ekb.eg/article_31404_5bcb585e77f608b370370cfd6bc2d1f3.pdf
Benha University; Faculty of Veterinary Medicine
Benha Veterinary Medical Journal
1110-6581
2974-4806
30
1
2016
03
01
Effect of Grilling on Pesticides Residues in O. niloticus Muscles
348
357
EN
Fatin
S.
Hassanen
Food hygiene Dept., Faculty of Veterinary Medicine, Benha University
Kamel
E.
A.
Animal Health Research Institute (El-Dokki - Biochemistry Dept.)
Rehab
A.M.
Gaafar
Animal Health Research Institute (Benha Branch – Food Hygiene Dept.)
Shaheen
A.
A.
Fish diseases & management Dept., Faculty of Veterinary Medicine, Benha University
10.21608/bvmj.2016.31405
Thirty six random healthy Oreochromusnilotica (O. niloticus) collected from Manzala Lake, wild Nile<br />River "El-Ryiah El-Tawfiky", El-Abbasa private fish farm and Gesrbahr el-baar agricultural drainage<br />(9 fish from each source). Concentrations of organochlorine pesticide residues (OCPs) were investigated<br />in both raw and grilled fish (dry heat treatment). Fish musculature samples were analysed for detection<br />the effect of grilling on level of organochlorine pesticides residues. Examination revealed that, samples<br />collected from El-Ryiah El-Tawfiki recorded the highest concentration of organochlorine pesticide<br />residues in raw O. niloticus followed by Manazla Lake, El-Abbasa fish farm and Gesrbahr el-baar<br />agricultural drain, respectively. Application of grilling revealed a great reduction in organochlorine<br />pesticides residues concentrations by ratio of 11% to 100% depending upon the type of pesticide residue,<br />its sensitivity to heat and locality of fish collection. In some cases thermal treatment showed unexpected<br />results represented by appearance of some residues which not detected in raw samples. This study<br />concluded that fish from some water resources in Egypt is highly contaminated with organochlorine<br />pesticide residues which greatly supposed to come from agricultural contaminated water. Moreover,<br />grilling is a good and highly recommended method to reduce organochlorine residues with uneven<br />degrees in fish muscles before consuming.
Organochlorine pesticide residues,O. niloticus,fish muscles,grilling heat treatment
https://bvmj.journals.ekb.eg/article_31405.html
https://bvmj.journals.ekb.eg/article_31405_332ef9545c921ce12ff157973fa6d79f.pdf
Benha University; Faculty of Veterinary Medicine
Benha Veterinary Medical Journal
1110-6581
2974-4806
30
1
2016
03
01
Biochemical Study on antioxidant after High Effort
358
367
EN
Abdel-Maksoud
A.
Hussien
Department of Biochemistry, Faculty of Veterinary Medicine, Benha University, 13736 Moshtohor, Qalioubeya, Egypt
Mahmoud
Rabie
Ahmed
Department of Biochemistry, Faculty of Veterinary Medicine, Benha University, 13736 Moshtohor, Qalioubeya, Egypt
Mohammed
A.
Hussein
Biochemistry Department, Faculty of Pharmacy, October 6th University, October 6th city, Egypt
Naglaa
Abd El-Khalik
Gobba
Department of Pharmacology, Faculty of Pharmacy, Misr University for Science and
Technology (MUST), October 6th City, Egypt
10.21608/bvmj.2016.31406
The present study was designed to evaluate hepatoprotective effects of Cranberry extract (75 and<br />150mg/kg.b.w) against Diclofenac sodium induced liver toxicity in rats. Oral administration of<br />Diclofenac sodium (150mg/kg.b.w.) led to significant increase in plasma Transaminases (L-Alanine and<br />L-Aspartate), Lactate dehydrogenase (LDH), Alkaline phosphatase (ALP), Tumor Nicroses Factor-Alfa<br />(TNF-α), Nitric oxide (NO) and TBARS as well as plasma, Triacylglycerol, Total Cholesterol, and LDLCholesterol.<br />Also, treatment of rats with Diclofenac sodium led to significant decrease in liver GSH,<br />Protein Thiols (Pr-SHs), Superoxide Dismutase (SOD), Catalase (CAT) as well as plasma HDL. The<br />obtained result revealed that cranberry extract (75 and 150mg/kg.b.w.) prevent liver tissue damage<br />through increasing of GSH, SOD and CAT activities and decrease significantly TBARs level. These<br />results suggest that, cranberry may be effective in enhances the protection of heart toxicity by its radical<br />scavenging effect and antioxidant activity
diclofenac sodium,liver toxicity,cranberry,Antioxidant enzymes,Lipid profile,GSH
https://bvmj.journals.ekb.eg/article_31406.html
https://bvmj.journals.ekb.eg/article_31406_7532955277432eb6790aeba18c736e80.pdf
Benha University; Faculty of Veterinary Medicine
Benha Veterinary Medical Journal
1110-6581
2974-4806
30
1
2016
03
01
Molecular and biochemical study on the potential therapeutic effect of novel composite on the hepatocellular carcinoma-induced rats
368
379
EN
Omayma
A.
Ragab
Biochemistry Department, Faculty of Vet. Medicine, Benha University, Egypt
Yakout
A.
El- Senousy
Biochemistry Department, Faculty of Vet. Medicine, Benha University, Egypt
Abdel Fattah
M.
Badawy
Applied Chemistry Department, Egyptian Petroleum Research Institute, Egypt
Ahmed
Mohammed
Rashad
Biochemistry Department, Faculty of Pharmacy, October 6 University, Egypt
10.21608/bvmj.2016.31407
The hepatoprotective effect of basic curcumin and bis (tetrachlorocuprate -lysine) combined with<br />ascorbate against ferric nitrilotriacetate (Fe-NTA) and chloroform induced hepatic carcinogenesis in rats<br />was evaluated. One hundred rats were divided into five equal groups. Normal control group,<br />carcinogenic (Fe-NTA: 9 mg Fe/kg b.wt. i.p.) and chloroform (150 mg/ kg b.wt. orally)-induced group,<br />curcumin group (400 mg/kg. b.wt. orally), tetrachlorocuprate-lysine (25 mg/kg. b. wt. s.c.) and ascorbate<br />(500 mg/kg. b. wt. orally) group and a mixture group (composed of curcumin, tetrachlorocuprate -lysine<br />and ascorbate). Blood samples and liver tissue specimens were collected at the end of experiment (4<br />months) for determination of the following parameters: activities of serum malondialdehyde (MDA),<br />reduced glutathione (GSH), tissue catalase, myeloperoxidase (MPO), Tumor necrosis factor-α (TNF- α<br />), interleukin-1, nuclear factor kappaB65 (NFkB65) and caspase-3. The obtained results showed that<br />injection of carcinogen caused a significant elevation in the MDA, MPO and immunological markers<br />levels, and significant reduction in the reduced glutathione, and catalase compared to the control group.<br />Compared to carcinogenic group, rats administered with the treated compounds resulted in a significant<br />reduction of MDA, MPO and immunological markers levels, and increased in reduced glutathione and<br />catalase activity. These results concluded that basic curcumin, tetrachlorocuprate-lysine and ascorbate<br />exert chemopreventative effect against hepatocellular carcinoma
Ferric nitrilotriacetate,Curcumin,Hepatoprotective,antioxidant,Carcinogen
https://bvmj.journals.ekb.eg/article_31407.html
https://bvmj.journals.ekb.eg/article_31407_b34fefc4ccbcb52fb6473c286934b179.pdf
Benha University; Faculty of Veterinary Medicine
Benha Veterinary Medical Journal
1110-6581
2974-4806
30
1
2016
03
01
Generating LacZ-reporter transgenic mice to identify α1 (XIX) Collagen (Col19a1) expression in Dermal Papilla Cells
380
395
EN
Ahmed
Abo-Ahmed
0000-0002-2300-9839
Laboratory of Molecular Design and Synthesis, Department of Regenerative Medicine and Applied Medical Sciences, Graduate School of Medicine, Gifu University
ahmed.ibrahim@fvtm.bu.edu.eg
Dong-soo
Lee
Laboratory of Molecular Design and Synthesis, Department of Regenerative Medicine and Applied Medical Sciences, Graduate School of Medicine, Gifu University, 1-1 Yanagido, Gifu 501-1194, Japan.
Masatake
Osawa
Laboratory of Molecular Design and Synthesis, Department of Regenerative Medicine and Applied Medical Sciences, Graduate School of Medicine, Gifu University, 1-1 Yanagido, Gifu 501-1194, Japan
masaosaw@gifu-u.ac.jp
Xujun
Han
Laboratory of Molecular Design and Synthesis, Department of Regenerative Medicine and Applied Medical Sciences, Graduate School of Medicine, Gifu University, 1-1 Yanagido, Gifu 501-1194, Japan
Md
Nojebuzzaman
Laboratory of Molecular Design and Synthesis, Department of Regenerative Medicine and Applied Medical Sciences, Graduate School of Medicine, Gifu University, 1-1 Yanagido, Gifu 501-1194, Japan
Foad
Farrag
Department of Anatomy and Embryology, Faculty of Veterinary Medicine, Kafr Elsheikh University, Kafr Elsheikh 33516, Egypt
Mohamed
Attia
Department of Anatomy and Embryology, Faculty of Veterinary Medicine, Benha University, Moshtohor, Toukh 13736, Egypt
mohamed.mitwalli@fvtm.bu.edu.eg
Hatem
Bahgat
0000-0001-7061-8496
Department of Anatomy and Embryology, Faculty of Veterinary Medicine, Benha University, Moshtohor, Toukh 13736, Egypt
amanyabbass65@gmail.com
Ahmed
Kassab
Department of Anatomy and Embryology, Faculty of Veterinary Medicine, Benha University, Moshtohor, Toukh 13736, Egypt
ahmed.kassab@fvtm.bu.edu.eg
10.21608/bvmj.2016.31408
It is widely accepted the concept that tissue morphogenesis is mediated by reciprocal interactions<br />between epithelial and mesenchymal cells, whereas the molecular details of these interactions remain<br />largely elusive. The hair follicle (HF) is a mini-organ whose proper morphogenesis is governed by a<br />series of interactions between epidermal and dermal cells. Due to its relatively simple structure, the HF<br />affords an excellent model to decipher the molecular mechanisms of epithelial – mesenchymal<br />interactions. To explore the molecular mechanisms by which Dermal Papilla (DP) cells regulate hair<br />follicle formation, we performed a comparative transcriptome analysis and identified numerous genes<br />preferentially expressed in the DP cells. Among these, a gene encoding type XIX Collagen (Col19a1)<br />has become the focus of our attention because of its extensive evolutionary conservation. We generated<br />Col19a1<sup>LacZ</sup> reporter mice using CRISPR/Cas9 system to induce homologous recombination to<br />recapitulate Col19a1 expression pattern. In mouse embryos, Col19a1<sup>LacZ</sup> expression is confined to the<br />DP cells of hair follicles, ring sinus of whiskers, skeletal muscles and basal keratinocyte layer of skin<br />epidermis of limbs and tail. Moreover, Col19<sup>LacZ</sup> started to be expressed at E14.5 in the dermal<br />condensates of hair germ stage to the DP cells of mature hair follicle but not in the placode stage.<br />Ongoing generation of Col19a1 null mice will ultimately explore the functional role of Col19a1 during<br />HF morphogenesis. From this perspective, it is plausible to expect that future studies will provide<br />additional functional evidence for Col19a1 during development which might be a useful tool to<br />understand the molecular mechanisms undergoing HF stem cell regulation
mesenchymal,morphogenesis,Col19a1,Dermal papilla,CRISPR/Cas9,Evolutionary
https://bvmj.journals.ekb.eg/article_31408.html
https://bvmj.journals.ekb.eg/article_31408_ced7c6e955489759f5caec94303dc3de.pdf