TY - JOUR ID - 268379 TI - Molecular Characterization And Antimicrobial Effect Of Some Antibiotics On Aeromonas hydrophila Isolated From Different Sources JO - Benha Veterinary Medical Journal JA - BVMJ LA - en SN - 1110-6581 AU - nasser, heba AU - abdeltawab, ashraf awad AU - maroof, ahmed AU - elkhyat, manar AD - faculty of vet med AD - Animal Health Research Institute "Benha branch" ARC AD - vet med Y1 - 2022 PY - 2022 VL - 43 IS - 1 SP - 45 EP - 50 KW - Aeromonas hydrophila KW - different sources KW - Genotypic Characterization KW - Multi-drug Resistant DO - 10.21608/bvmj.2022.153716.1567 N2 - The distribution of drug resistance among A. hydrophila was evaluated around the world and showed the development of this pathogen as one of the major opportunistic human pathogens. So, this study was aimed to evaluate the antimicrobial resistance of 44 A. hydrophila isolates previously isolated from 225 random samples of cat fish; cow's milk; beef; ground water and diarrheic human stool of patients collected from different fish markets; shops; various localities and hospitals (45 for each), at Kaliobia Governorate Egypt, beside detection of some antimicrobial resistant virulence genes in some strains. The results revealed that, all 44 isolates were resistant for ampicillin; then they were highly resistant for methicillin and oxacillin followed by amoxicillin; cefotaxime and tetracycline; streptomycin and co- trimoxazole. Meanwhile, they were highly sensitive to meropenem followed by norfloxacin then ciprofloxacin; gentamycin; florphenicol and doxycycline. Moreover, PCR cleared that, blaTEM and bla CTX-M genes were detected in seven out of eight studied strains giving products of 516 bp. &593 bp., respectively; sul1and aadA1 genes were detected in all eight studied strains giving products of 433 bp. &484 bp., respectively and tet A(A) gene was detected in sex out of eight studied strains giving products of 576 bp. Therefore, this study concluded that, the presence of antimicrobial resistant A. hydrophila strains in foods of animal origin (meat, milk) ,cat fish and ground water could be a public health concern for the consumers. UR - https://bvmj.journals.ekb.eg/article_268379.html L1 - https://bvmj.journals.ekb.eg/article_268379_6b731f666e99e8c424bb3b402969bb12.pdf ER -