2024-03-29T02:44:29Z
https://bvmj.journals.ekb.eg/?_action=export&rf=summon&issue=15699
Benha Veterinary Medical Journal
1110-6581
1110-6581
2020
38
1
Proanthocyanidin ameliorates against ethanol -induced gastric mucosal erosion by attenuating inflammatory response, oxidative stress and apoptosis in rats
Reem
Obaia
Yakout
Elsenosi
Mohamed
Mahfouz
Samy
Aziza
Gastric ulcer is a common chronic disease in human digestive system. Massive alcohol drinking can lead to gastric ulcer. The gastroprotective effect and molecular mechanisms of Proanthocyanidin in a rat model of ethanol-induced gastric mucosal erosion were investigated.35 male rats were divided into five equal groups. Group 1 :( Control normal) rats received no drugs. Group 2:(Early ulcer) rats received absolute ethanol (0.5ml/100g rat) orally on empty stomach and sacrificed one hour later. Group 3 :(Early ulcer + proanthocyanidin protected) rats received proanthocyanidin orally (300mg/kg b.wt/day) for 3 weeks before ethanol administration then sacrificed after one hour. Group 4: (Late ulcer) rats received ethanol similar to group 2 and sacrificed after 21 days. Group 5: (Late ulcer + proanthocyanidin treated) rats first administered with ethanol (0.5ml/100g rat) and after one hour proanthocyanidin was administered for 21 days. A significant increase in stomach L-MDA concentration with marked decrease in CAT activity and GSH concentration were observed in gastric erosion -induced rats. However, a significant depletion of gastric L-MDA level and marked increase in CAT activity and GSH concentration were observed after Proanthocyanidin treatment when compared to ulcerated rats. A significant up-regulation of gene expression level of BAX, NF-κB and IL-1β with down-regulation of Bcl-2 gene were observed in stomach of gastric erosion -induced rats. However, a significant down regulation of BAX, NF-κB and IL-1β with up-regulation of Bcl-2 gene were observed after Proanthocyanidin treatment. Conclusively, Proanthocyanidin protects rat gastric mucosa against ethanol-induced gastric erosion via anti-inflammatory, anti- apoptotic and anti-oxidative mechanisms.
Proanthocyanidin
gastric erosion
Oxidative Stress
inflammatory mediators, apoptosis
2020
03
01
1
8
https://bvmj.journals.ekb.eg/article_103454_0d28548f7afdc7f9ec93d29c8a068859.pdf
Benha Veterinary Medical Journal
1110-6581
1110-6581
2020
38
1
Novel role of probiotics in improving cell proliferation and regulating proinflammatory cytokine-mediated oxidative damage of ethanol-induced gastric mucosal injury in rats
Hagar
Youssef
Yakout
Elsenosi
Mohamed
Mahfouz
Samy
Hussein
Gastric ulcer is a common chronic disease in human digestive system. Excessive alcohol drinking can lead to gastric ulcer. The gastroprotective effect and molecular mechanisms of probiotics in a rat model of ethanol-induced gastric injury were evaluated. Thirty five rats divided into five equal groups. Group 1 :( Control normal) rats received no drugs. Group 2 :(Early ulcer) rats received absolute ethanol (0.5ml/100g rat) orally on empty stomach and sacrificed one hour later. Group 3 :( Early ulcer + Probiotics protected) rats received probiotic orally (135 mg/kg b.wt/day) for 3 weeks before ethanol administration then sacrificed after one hour. Group 4: (Late ulcer) rats received ethanol similar to group 2 and sacrificed after 21 days. Group 5: (Late ulcer + Probiotics treated) rats first administered with ethanol and after one hour probiotic was administered for 21 days. A significant increase in L-MDA with marked decrease in GSH and CAT were shown in stomach of gastric injury-induced in rats compared with control group. Conversely, a significant decrease in L-MDA and obvious increase in GSH and CAT were observed after probiotics treatment compared to ulcerated rats. Likewise, a significant up-regulation of NF-κB gene expression level was observed in stomach of gastric injury-induced in rats. This expression was downregulated after probiotics administration. Meanwhile, a significant down-regulation of PPARγ and TGF-β1 gene were detected in gastric injury-induced rats. This expression was upregulated after probiotics administration. Conclusively, probiotics improving gastric cell proliferation and protect gastric mucosa against injury-induced in rats via anti-inflammatory and anti-oxidative mechanisms.
probiotics
gastric injury
Oxidative Stress
proinflammatory cytokine
PPARγ
2020
03
01
9
16
https://bvmj.journals.ekb.eg/article_103453_ec77dfa91b2365ffc9c268c0f0862b04.pdf
Benha Veterinary Medical Journal
1110-6581
1110-6581
2020
38
1
Pathological Studies on lung affections in sheep and goat at Kalubia Governorate
Abdel-Baset
El-Mashad
Shawky
Moustafa
Aziza
Amin
Eman
Samy
The gross and histopathological changes in the lungs of small ruminants at Kalubia Governorate were investigated. A total of 159 lung samples (134 sheep and 25 goats) showed gross lesions were collected from February 2018 till November 2019. Bacterial strains were isolated from 26 cases out of 159 (16.35%) including various types where Staphylococcus aureus was the most predominant isolated strain from the lungs. Based on histopathological examination, different types of pneumonia (28.9%) including; suppurative pneumonia (10.69%), fibrinous pneumonia (7.54%), interstitial pneumonia (6.28%), granulomatous pneumonia (1.26%) and verminous pneumonia (3.14%) were detected. Bronchitis and bronchiolitis (21.38%); pleural fibrosis (4.4%) were recorded. Pulmonary adenomatosis and fibrosarcoma were detected in lungs of sheep with an incidence of 2.52% and 1.26% respectively. The study concluded that pneumonia is an important lesion in the lungs of small ruminant, and suppurative Pneumonia was the most predominant type. Verminous Pneumonia was detected only in goats and not detected in sheep. Pulmonary adenomatosis were recorded in lungs of sheep but not observed in goat.
histopathology
lung affections
small ruminant
Kaluabia
2020
03
01
17
23
https://bvmj.journals.ekb.eg/article_103445_9c562f4a9c29919d25250fb4ebad2f0b.pdf
Benha Veterinary Medical Journal
1110-6581
1110-6581
2020
38
1
Evaluation of some heavy metals’ residues in tilapia
Sarah
Khalifa
Mohamed
Hassan
Reham
Amin
Nabil
Marzouk
Heavy metal (Mercury, lead, and cadmium) residues was appraised in various body weights of Tilapia fish gathered randomly from various regions at Cairo and Giza markets utilizing Atomic Absorption Spectrophotometer (AAS). The average estimations of mercury, lead, and cadmium residues in tilapia at weight up to 200 gm was 0.07±0.01, 0.1±0.02 and 0.0005±0.0001 ppm, respectively. While, the average values of mercury, lead, and cadmium residues in tilapia at weight 200-400 gm was 0.3±0.05, 0.2±0.02 and 0.03±0.01 ppm, respectively. Whereas, the mean values of mercury, lead, and cadmium deposits in tilapia at weight 400-600 gm was 0.6±0.06, 0.34±0.03 and 0.05±0.01 ppm, respectively. Heavy metal residues are positively correlated with fish size. Lead was the most elevated metal in tilapia up to 200gm. While Mercury was the highest metal in tilapia 200-600 gm. The outcomes were assessed by Egyptian Organization for Standardization EOS (NO. 7136/2010) and the public health hazards of heavy metal residues was debated.
heavy metal
mercury
cadmium
Lead
tilapia
2020
03
01
24
28
https://bvmj.journals.ekb.eg/article_103448_c3f5760584953422f298c3b88d1dc804.pdf
Benha Veterinary Medical Journal
1110-6581
1110-6581
2020
38
1
Antioxidant, Sensory and Antibacterial activities of some Essential oils in Beef Kofta
Elham
Hosny
Reham
Amin
marionette
Nassif
This study was investigated the antioxidant, antibacterial and sensory effects of lemongrass (Cymbopogon Citrates), orange (Citrus Sinensis) and thyme (Thymus vulgaris) oils in concentrations (0.5% & 1%) on beef Kofta at 4oC for 10 days storage period. All essential oils used had considerable effectiveness in pH, thiobarbituric acid reactive substance (TBARs) and total volatile basic nitrogen (TVBN) values as well as aerobic plate count (APC), Coliform count, Staphylococci count and Staphylococcus aureus count. Lemongrass, orange and thyme oils at two concentrations improved sensory properties of kofta. Lemongrass oil in two concentrations (0.5% & 1%) showed the best results as antibacterial and antioxidant, and also gave good organoleptic properties, but lemongrass oil 0.5% was more accepted. Orange oil 0.5% showed the lowest results as antibacterial and antioxidant with accepted organoleptic properties. On other hand orange oil 1% is more effective as antibacterial and antioxidant and demonstrated more enhancement of sensory attributes. Thyme oil in two concentrations (0.5% & 1%) was effective as antibacterial and antioxidant. Thyme oil at concentration 1% has unacceptable organoleptic properties in kofta, only thyme oil 0.5% can be used. So, we suggest that lemon grass oil 0.5%, thyme oil 0.5% and orange oil 1% can be applied to kofta as natural preservatives.
Essential oils
kofta
antibacterial and antioxidant
2020
03
01
29
34
https://bvmj.journals.ekb.eg/article_103455_debab284f3aab363c26e329756e9e64c.pdf
Benha Veterinary Medical Journal
1110-6581
1110-6581
2020
38
1
Chemical quality of marketed freshwater fish in Qualiobeya governorate,Egypt
Walaa
Abdelazeem
Mohamed
Hassan
Abd ElAziz
Barr
Determination of changes in potential of hydrogen ( pH),total volatile nitrogen (TVN) and trimethylamine (TMA) in three types of fish (Oreochromis niloticus ,Clarias lazera and Mormyrus niloticus ) 30 of each collected from markets of El Qualiobeya governorate,Egypt. The results revealed that The mean value of pH in examined samples were 6.36 ± 0.05 ; 6.19 ± 0.03 and 6.07 ± 0.02 for Oreochromis niloticus ,Clarias Lazera and Mormyrus niloticus respectively. The mean value of Total Volatile Nitrogen (TVN) were 21.58 ± 0.49 mg% ; 16.73 ± 0.41 mg % and 14.15 ± 0.28 mg% for Oreochromis niloticus , Clarias Lazera and Mormyrus niloticus respectively. The un accepted samples were 16.67%, 6.67% and 3.33% in Oreochromis niloticus,Clarias lazera and Mormyrus niloticus respectively. Trimethyl Amine(TMA) values were 8.85 ± 0.22 mg% for Oreochromis niloticus; 6.61 ± 0.15 mg% for Clarias lazera and 5.97 ± 0.13 mg% for Mormyrus niloticus . The un accepted samples were 20% ,13.33% and 3.33% in Oreochromis niloticus, Clarias lazera and Mormyrus niloticus respectively.
Potential of hydrogen
total volatile nitrogen
trimethylamine
Nile Fish
2020
03
01
35
38
https://bvmj.journals.ekb.eg/article_103449_a0fa019ec65434410fdcad180856f063.pdf
Benha Veterinary Medical Journal
1110-6581
1110-6581
2020
38
1
Demonstration of histamine in Nile fish
walaa
abdelazeem
AbdElazez
Helmy
Mohamed
Hassan
Demonstration of histamine in nile fish Food Hygiene and Control Department, Fac. Vet. Med., Benha University* ABSTRACT In this study, we determine histamine concentration in three types of fish (Oreochromis niloticus , Clarias lazera and Mormyrus niloticus ) 30 of each collected from fish markets of El Qualiobeya governorate,Egypt ,The results revealed that the mean value of histamine in the examined samples were 18.23 ± 1.56 mg/kg ; 14.90 ± 1.21 mg /kg and 12.65 ± 1.07 mg /kg for Oreochromis niloticus, Clarias lazera and Mormyrus niloticus respectively. According to Egyptian Organization of Standardization which recommended that the critical limits for histamine should not be more than 20mg/100g in fish, the un-accepted samples represented as 26.67 %,16.67 % and 13.33 % in Oreochromis niloticus , Clarias lazera and Mormyrus niloticus , respectively .So,all samples are acceptable. Although biogenic amines (Bas) formation is the result of bacterial growth, the presence of these undesirable compounds, especially histamine, is not always noticed by consumers. Thus, histamine is the main marker for the evaluation of quality and safety of fish.
Keywords
Histamine ,Niloticus ,Nile fish ,Clarias Lazera
2020
03
01
39
42
https://bvmj.journals.ekb.eg/article_103447_118c9fc89647d1b5843e6bb43ced1740.pdf
Benha Veterinary Medical Journal
1110-6581
1110-6581
2020
38
1
Determination of Biogenic amines in hard cheeses by High performance liquid chromatography
Yasmin
Abd El- Salam
Madeha
Hassan
Salah
Abd El-Aal
Rania
Kamal
Yasmin
Ahmed
Marionette
Nasseif
This study evaluated chemically the quantity of six biogenic amines "histamine, tyramine, puterscine, cadaverine, tryptamine and phenylethylamine" in forty hard cheese samples (Chedder and Ras) (20 of each) sold in retail markets in Zagazig City Sharkia Governorate using high performance liquid chromatography (HPLC) and correlation with compositional quantity of cheese (pH, salt%, moisture% and acidity%). Our results showed the mean values of pH, salt, moisture and acidity were (5.5±0.02 and 4.79±0.11), (1.53±0.04 and 4.42±0.05%), (40.45±0.24 and 38.64±0.34%) and (0.84 ±0.01 and 1.70±0.04%) in Chedder and Ras cheese samples, respectively. The mean values of total biogenic amines were 12.65±2.7and 28.95±4.6 mg/100g in Chedder and Ras cheese samples, respectively. There was a negative correlation between (pH value and moisture %) and biogenic amines production but the positive correlation was found between (salt and acidity %) and biogenic amines production. Finally it was found that all Chedder and Ras cheese samples were accepted according to maximum permissible limit of Egyptian organization standard (EOS, 2007) which is 20mg/100g. All samples were accepted according to maximum permissible limit of Food and Drug Administration (FDA, 2001) which is 10mg/100g except 60 and 90 % of Ras cheese samples are accepted for histamine and puterscine, respectively. On conclusion, optimization and standardization of milk quality and hygiene during cheeses manufacturing and storage should be taken for human safety.
biogenic amines
Chedder and Ras cheese
HPLC
2020
03
01
43
47
https://bvmj.journals.ekb.eg/article_103451_98122901b6b8715b2eb97457a57d8ecc.pdf
Benha Veterinary Medical Journal
1110-6581
1110-6581
2020
38
1
Diversity of some food born fungi associated with raw milk and some cheese in Egypt
Ashraf
Abd El Tawab
Fatma
El-Hofy
Eman
EL-diasty
Ebtehal
Abo-Hamdah
Manar
Al-Khayat
The contamination of milk and cheese with different types of fungi would constitute a public health hazard . These fungi may cause mycotoxicosis in human and/or spoilage of these products. This study aimed to the isolation and identification of mould and yeast in raw milk and some cheese. Sixty samples were collected from Giza Governorate, Egypt including; 20 raw milk, 20 processed cheese and 20 soft white cheese. The results reported that, overall incidence rate of the moulds in processed cheese, soft white cheese and raw milk were; 18/20 (90%), 6/20 (30%) and 16/20 (80 %), ,respectively while the mean mould counts were 1.3 × 103 ± 1.8× 10² cfu/g ,6.7 × 103 ± 2.6× 103 cfu/g and 5.9 × 103 ± 1.3× 103cfu/ml,,respectively .The isolated mould species from these samples were Aspergillus spp., Penicillium spp., Eupenicillium spp. ,Phoma spp., Geotrichum spp. and Fusarium spp. while, the yeast contamination was detected in soft white cheese and raw milk samples with incidence rate of 10/20 (50%) and 16/20 (80%); respectively, while not detected in processed cheese samples. The most predominant yeast species isolated from soft white cheese and raw milk samples were Debaryomyces hansenii, Pichia anomala, Sacharomyces cervisioe ,Candida tropicalis, Candida pseudotropicalis, Torulopsis and Rhodotorula. In conclusion, the presence of high incidence of fungi in raw milk and cheese samples would lead to a public health hazards and/or economic losses due to spoilage of these products, therefore, strict hygienic conditions are required. Keywords: Moulds, Yeasts, Raw milk , Cheese, Egypt.
moulds
yeasts
Raw milk
cheese
Egypt
2020
03
01
48
51
https://bvmj.journals.ekb.eg/article_103450_d3aba811839d98959c9a9a6f058c8bc9.pdf
Benha Veterinary Medical Journal
1110-6581
1110-6581
2020
38
1
Comparison of the efficacy of local and imported inactivated combined H9-ND virus vaccines in protection of broiler flocks against H9N2 infection in Egypt
haitham
Ebrahim
manar
Seioudy
The low pathogenic avian influenza becomes endemic in Egypt since its first record in 2011. It affected all the chicken sectors. There are some commercial vaccines either imported or local vaccine. This study conduct to compare the effectiveness of two H9ND vaccines, one imported and the other local, through two experiment the first in the laboratory using 150 one day old broiler chicks divided into 3 groups , the first two groups were vaccinated by the imported and local vaccine, respectively at one day old, the third group kept as negative control , the antibodies against H9N2 virus were measured by HI test at 14,21 and 28 day old , at 21 day old each group were divided to two group the 1st kept unchallenged, the 2nd challenged with H9N2 virus using the circulating local strain , in the chicken isolators ,cloacal and tracheal swaps were taken at 2,4and 6 days post challenge to detect the shedding virus using real time RT- PCR test. The second experiment were in a commercial broiler flock contain 432,000 chickens placed in 16 pens. The broiler chickens divided into 2 groups, each group took a different commercial H9ND vaccine at 1st day, the humeral immunity measured, at 14,21and 28 day old. . The results showed that the locally produced vaccine provide significant higher immune response in both lab. and field experiment ,on the other side the virus shedding is stopped early in the group vaccinated by local vaccine.
influenza
Local vaccine
Imported vaccine
2020
03
01
52
56
https://bvmj.journals.ekb.eg/article_103425_846fbcef4566e5525f29e83b6fe42e1d.pdf
Benha Veterinary Medical Journal
1110-6581
1110-6581
2020
38
1
Seroprevalence detection of Coxiella burnetii antibodies in milk and serum of dairy cattle by recent methods
rihan
salem
Fatma
Elhofy
Mona
Hassaballa
Ashraf
Abd El Tawab
This study aimed to detect C. burnetii antibodies in milk and serum of dairy cattle using PCR compared with other immunological techniques namely IFAT and ELISA. These samples were collected from apparently healthy cows (n=300) from Giza, Fayoum and Beni Suief Governorates. Real-time PCR for detection of C. burnetii DNA was performed on samples using two individual PCR assays with specific primers and probes to increase specificity of the result using Roche kits. IFAT revealed detection of 6% IgM antibodies in milk and 7.3% in sera. While IgG was 10.6% of milk and 24% in sera. The main value of positive samples by ELISA test were 25.7%; in serum 34% and in milk 17.3%. These results showed that sensitivity and specificity of the ELISA test and IFAT were highly comparable. The result of Real time PCR from 77 positive samples of C. burnetii was 45.4%. The highest results for real time PCR recorded 57.1% in Beni Suief followed by 44% in Giza and 33.3% in Fayoum. It was concluded that the apparently healthy dairy cows are an important reservoir of C. burnetii infection. Investigations on C. burnetii using PCR as well as serological surveys of animals are important methods for diagnosis and control of Q-fever. Awareness is needed for animal owners, veterinarians, physicians and authorities.
Coxiella burnetii
PCR
IFT
ELISA
2020
03
01
57
60
https://bvmj.journals.ekb.eg/article_103442_c7ae064890797ace7cf17196a24895d5.pdf
Benha Veterinary Medical Journal
1110-6581
1110-6581
2020
38
1
Detection of virulence factors of Mycoplasma species isolated from chicken by multiplex PCR
Mohammed
El.ordy
Ashraf
Abd-El-Tawab
Wafaa
Hassan
One hundred and sixty clinically diseased broiler local chickens collected from 22 different farms belonging to Qalubia, Dakahlia and Gharbia governorates were subjected to bacteriological examination and molecular characterization. Clinical Signs were chronic respiratory disease with respiratory manifestations,lameness, loss or reduction in egg production.Bacteriological examination showed that 18.18% of the isolates were Mycoplasma positive and showed growth in pleuropneumonia-like organism (PPLO) agar plates, with fried egg appearance when examined by stereoscopic microscope. Mycoplasma colonies were tested for antimicrobial sensitivity tests against 12 antimicrobial antibiotics and showed higher sensitivity to nitrofurantoin, gentamicin, norfloxacin, ciprofloxacin and neomycin. Intermediate sensitivity were recorded to ceftriaxone and cefotaxime and antibiotic resistance were recorded to tetracyclines, lincomycin, chloramphenicole and sulphamethoxasine. Identification of the bacterial strains of the isolates were conducted by multiplex PCR by using two primer pairs for Mycoplasma synoviae (vlhA) and Mycoplasma gallisepticum (mgc2). The amplicons expected size were 396 bp, and 300 bp for vlhA and mgc2 respectively. Only13 out of 22 farms were positive, representing 59.09 %.Moreover, the incidence rate of Mycoplasma synoviae (vlhA) and Mycoplasma gallisepticum (mgc2) were 22.72% and 13.63% respectively and 22.72% of the inspected farms showed positive results for both Mycoplasma strains.
MS (vlhA)
MG (mgc2)
PCR
Broiler chickens
sensitivity test
2020
03
01
61
65
https://bvmj.journals.ekb.eg/article_103440_4df93df89912aefdcf8886ac73ea69d4.pdf
Benha Veterinary Medical Journal
1110-6581
1110-6581
2020
38
1
Detection of Some resistance genes of Salmonella enterica subsp. Salamae and Salmonella enterica serotype Kentucky isolated from Turkey
Alaa
Gouda
Ashraf
Abd El-Tawab
Seham
Homouda
The aim of this study was to determine the serotyping and antimicrobial resistance of isolated Salmonella from the appearently healthy turkey. A total of 150 (cloacal samples) from living apparently healthy turkeys samples were screened bacteriologically for the occurrence of Salmonella. A total of 6/150 (4%) isolates of Salmonella was recovered. Serotyping revealed two different serotype, S.Enterica subspp Salamae (33.33%) and S.Kentucky.(66.67%). The isolated Salmonella were highly resistant to ampicillin, cefaclor, cefotaxime, ceftazidime, amoxicillin/clavulanic acid(100%) followed by chloramphenicol and ciprofloxacin (83.3%) then gentamycin (66.67%) and azithromycin (33.3%). All isolates showed high sensitivity for imipenem. All strains are multidrug-resistance (MDR). Polymerase chain reaction (PCR) was applied on Salmonella isolates to detect resistance gene. Antibacterial resistance genes blaTEM, blaOXA, floR, aadB and qnrA were detected in (100%), (0%), (100%), (100%) and (0%) of tested Salmonella. A combination of genotypic and phenotypic markers can be useful in studying genetic variation among salmonellae populations in turkey production and delineating possible transmission pathways. Conclusion: This study demonstrates that appearently healthy turkeys could be a reservoir for Salmonella resistant to multiple antimicrobials and poses a serious public health threat.
serotype
Salmonella
turkey
Resistance gene
2020
03
01
66
69
https://bvmj.journals.ekb.eg/article_103441_404c19cea04035b4432403f23c806f70.pdf
Benha Veterinary Medical Journal
1110-6581
1110-6581
2020
38
1
Prevalence of Clostridium perfringens infection and virulence genes molecular study in broiler chickens
Amira
Rizk
Ashraf
Abd El-Tawab
Mohammed
El- Bardisy
Shreen
Mayouf
Clostridium perfringens (C. perfringens) produces toxins that cause necrotic enteritis (NE) in broiler chickens resulting in sever losses in poultry industry. This study aimed to investigate the prevalence of C. perfringens in broiler chickens in Toukh, Qaliubiya governorate, Egypt. A total of 100 broilers (10-40 days old) collected from Toukh, Qaliubiya in 2017. The samples were collected from liver and intestine of both diseased and apparently healthy broilers. All samples were subjected to bacteriological examination. Clostridium Perfringens was isolated from (70%) and (22%) of diseased and apparently healthy broilers respectively. The isolates were typed by dermonecrotic test in Guinea pig and PCR. The results of dermonecrotic test from diseased chickens showed that 40 (65.6%) of the diseased isolates were type A and 8 (13.1%) were type D. But the isolates from the apparently healthy chickens were (9) type A and (6) type D. Typing of the 9 C.Perferingens isolates (diseased broilers) by PCR for toxin genes (cpa ,cpb, etx ) showed that 8 strains were positive for cpa gene (typed as type A). Detection of the virulence genes by PCR showed, pfoS and colA genes were in (8) isolates but netB gene was detected in (3) isolates only. Clostridium perfringens showed high prevalence among diseased broiler chickens with high isolation rate from intestinal sample than hepatic samples. Moreover, high percentage of C.perfringens are toxigenic type A in case of diseased or apparently healthy broiler chickens. The netB gene seems to be unnecessary for inducing necrotic enteritis in broiler chickens.
C.perfringens
Broiler chickens
netB gene
cpa gene
2020
03
01
70
74
https://bvmj.journals.ekb.eg/article_103443_e1c36eb34798d9994d604c5f806fc276.pdf
Benha Veterinary Medical Journal
1110-6581
1110-6581
2020
38
1
Prevalence of multi drug resistant Escherichia Coli in diarrheic ruminants
wafaa
el-shazly
Ashraf
Abd El-Tawab
Fatma
Elhofy
ahmed
el hamalawy
amgad
abo el ella
manar
el-khayat
Abstract: Escherichia coli is the most significant cause of neonatal diarrhoea in ruminants. The current study was designed to monitor the prevalence of multidrug-resistant E. Coli in diarrheic ruminants in Egypt. Rectal swab samples (n=150) were collected between November 2018 and April 2019 from diarrheic calves(n=35), lambs(n=35) and goat kids up to 3months(n=80) from Gimmeza animal production researches station, Agriculture Research Centre (ARC), Egypt. Samples were submitted for isolation and identification of E. coli by conventional culture methods. From the examined samples(n=150), eighty-two (54.67%) were positive for E. coli isolation. Among the 82 E. coli isolates,48 were identified as pathogenic E .coli by cultivation on Congo Red Agar to assess the resistance profile, the 48 pathogenic E. coli isolates were submitted for antibiogram sensitivity tests, which revealed that 10/48 (20.83%) isolates were multidrug-resistant (MDR). The 10 MDR. coli isolates were serologically identified as four (n=4) O157:H7isolated from calves (n=2) and goat kids (n=2) and three (n=3) O125isolated from calves (n=2) and lambs (n=1) and three (n=3) O44isolated from goat kids (n=2) and lambs (n=1).
Keywords: E. coli
Diarrhoea
sensitivity test
MDR
2020
03
01
75
78
https://bvmj.journals.ekb.eg/article_103444_9f341731e5cdc393aa607ec8e1504426.pdf
Benha Veterinary Medical Journal
1110-6581
1110-6581
2020
38
1
Effect of cetyltrimethylammonium bromide surfactant coated Curcumin copper nanocomposite on hepatocellular carcinoma in rats
Omayma
abo zaid
Afaf
Abdelmageed
Abdelfattah
Badawi
Asmaa
Eid
This study was designed to investigate the role of (Curcumin/ Copper / cetyltrimethylammonium bromide) nano particles complex against hepatocellular carcinoma (HCC) induced in rats. Forty rats were divided into four groups. Group 1(negative control group): normal rats. Group 2 (Diethylnitrosamine) (DEN): rats administrated orally DEN with a dose of 20 mg/kg b. wt., five times a week / 6 weeks for HCC induction. Group 3 (protective ): rats were orally treated with Curcumin/ CU / CTAB (dissolved in Tween-80 (1 %) in 0.9 % normal saline) at a dose of 35 mg/kg b. wt., five times a week / 15 weeks as 2 weeks before DEN then, during administration of DEN and 15 weeks after DEN administration. Group 4 )treated group) rats treated with Curcumin/ CU/ CTAB dissolved in Tween-80 (1 %) in 0.9 % normal saline), at a dose of 35 mg/kg b. wt., five times a week / 15 weeks, following the administration of DEN for 6 weeks . Injection of DEN induced a significant increase in serum level of alpha-fetoprotein (AFP), alpha-L-fucosidase activity (AFU) and vascular endothelial growth factor (VEGF). On other hand, marked depletion in tissue antioxidants including superoxide dismutase (SOD), glutathione perioxidase (GPX) activities, and reduced glutathione (GSH) concentration. Administration of (Curcumin/ CU/ CTAB) nano composite was able to prevent cellular damage induced by reactive oxygen species (ROS) and able to make changes of tumor markers and tissue antioxidants nearly toward control.
Hepatocellular carcinoma
Diethylnitrosamine
Curcumin
copper
Cetyltrimethylammonium bromide
2020
03
01
79
81
https://bvmj.journals.ekb.eg/article_103452_39fca7f6b44afd8bea4beeb1ca091dd9.pdf
Benha Veterinary Medical Journal
1110-6581
1110-6581
2020
38
1
Assessment of Organochlorine Pesticides Residues in Tilapia fish (Oreochromis niloticus)
Sarah
Khalifa
Mohamed
Hassan
Reham
Amin
Nabil
Marzouk
The current study aimed to determine organochlorine pesticides (OCPs) residues in muscle tissues of Tilapia (Oreochromis niloticus) with different weights collected randomly from different localities at Cairo and Giza markets by Gas Chromatoghraphy. The total mean values of DDT, DDD, DDE, Endrin, Dieldrin, Endosulfan, γ-chlordane, δ-BHC, Hepatochlor epoxide, Methoxychlor and Hepatochlor residues in the examined samples of tilapia were 16.82 ± 4.7, 44.80 ± 4.65, 8.34 ± 1.42, 26.54 ± 3.13, 10.91 ± 2.72, 6.20 ± 1.17, 21.32 ± 3.44, 16.17 ± 3.07, 5.33 ± 10.67 , 1.24 ± 0.31 and 1.28 ± 0.31 ng/g, respectively. Accumulation of organochlorine pesticides in fish tissues was related to rate of pollution, lipid content, feeding behavior, rate, size and age. There were significant differences between organochlorine pesticides residues and different weights of tilapia. Most of the examined samples were within the maximum permissible limits of pesticides residues according to US-FDA (2008) and Codex Alimentarius Commission (1996) and should not pose a health risk to consumers. The public health hazards of organochlorine pesticides residues were discussed.
organochlorine pesticides residues
muscle tissue
Tilapia (Oreochromis niloticus)
2020
03
01
82
87
https://bvmj.journals.ekb.eg/article_103457_125b582c2f2c9cf7602ab9e851bd4706.pdf