2024-03-29T00:42:47Z
https://bvmj.journals.ekb.eg/?_action=export&rf=summon&issue=26492
Benha Veterinary Medical Journal
1110-6581
1110-6581
2021
40
2
Clinical study on Moraxella-associated infectious keratoconjunctivitis (IKC) of small ruminants
ahmed
zaitoun
mohamed
Elseleny
khaled
El khabaz
Infectious keratoconjunctivitis (IKC) represents an important health problem in semi-desert and desert areas. The present study tries to investigate the possible role of Moraxella as a causative agent of IKC. A total number of 140 sterile conjunctival swabs from the diseased animals (93 sheep and 47 goats) of different ages showed signs of IKC were subsequently collected, bacteriologically examined to isolate Moraxella spp, which was successively isolated from 19 animals (11 sheep and 8 goats) with infection rate about 13.6%. In relation to age, a higher infection rate (19.2%) was recorded among sheep in the age group ≥ 12 to < 24 months and among goats in the age group ≥ 3 to < 6 months. Antimicrobial sensitivity test of obtained isolates revealed that Moraxella was 100% sensitive to Ciprofloxacin, Gentamicin, and Tylosin, and 79% to Oxytetracycline. All tested isolates were resistant to Vancomycin, Ampicillin plus Sulbactam, and Sulfamethoxazole plus Trimethoprim. Therapeutically, two therapeutic regimens were carried out to treat the diseased cases under field conditions and the results were illustrated in detail.
Infectious keratoconjunctivitis
Moraxella
Small ruminants
antimicrobial sensitivity test
Field therapeutic trials
2021
07
01
1
4
https://bvmj.journals.ekb.eg/article_183341_8c2096924edec5bdb266d5afec8cd7f2.pdf
Benha Veterinary Medical Journal
1110-6581
1110-6581
2021
40
2
Immunogenicity of inactivated Equine Influenza (H3N8) virus vaccine with different adjuvents in equine
Fatma
Warda
eman
shosha
aml
abdelraouf
Magda
Anes Kalad
Equine influenza virus (EIV), is of the important commen and important respiratory infectious diseases of horses. Vaccination can reduce the the prevalence and severity of disease. In the current study, a trial to improve the strength and duration of immune response through using of montanide oil ISA 206, saponin and Mycobacterium pheli extract as adjuvants for an egg grown inactivated EIV [A/equine/Alexandria/1/08 (H3N8)]. Safety and potency of the prepared vaccine studied by inoculation into guinea pigs and horses then evaluated using Haemagglutination inhibition (HI) test for humeral immune response. Cellular immune response evaluated in horses inoculated with inactivated EIVvaccine adjuvanted with Mycobacterium pheli extract using lymphocyte blastogenesis. The vaccines were safe for g.pigs and horses which inducing HI antibodies titer that persist for 10 months post-inoculation in vaccinated horses. EI vaccine adjuvanted with montanide oil ISA 206 was more potent than the other vaccines adjuvanted either with saponin or M.pheli extract. EI vaccine adjuvanted with montanide oil ISA 206 also keeps at 4oC for over one year and at room temperature for 6 months without effect on its immunogenic characters.
Equine influenza virus (H3N8)-Horses
inactivated vaccine
adjuvants
2021
07
01
5
11
https://bvmj.journals.ekb.eg/article_185807_4c87cfe4a6d80eeeb91ddceef04ac178.pdf
Benha Veterinary Medical Journal
1110-6581
1110-6581
2021
40
2
Molecular characterization of salmonella species and E.coli isolated from dogs and cats
Reham
Mekky
Abdel-Moneim
Moustafa
Rania
Abo-Sakaya
Khairy
Abu-Zaid
In this study we aimed to investigate salmonella and E.coli serovars from fecal swabs collected from apparently healthy and diarrheic dogs and cats by bacteriological examination. 150 fecal samples of dogs and cats were examined for salmonella and E.coli species. Biochemical test are done to confirm suspected colonies as VP (Voges Proskauer), MR (Methyl red), indole reaction, citrate utilization, catalase test and sugar fermentation test. Salmonella species were isolated from 40 cases out of 150 , 30 of the salmonella positive samples were from dogs (31.6%), and 10 were from cats (18.2%) while 45 samples were positive for E.coli, 25 from dogs (26.4 %) and 20 from cats (36.4 %). E.coli and salmonella positive samples were subjected to antimicrobial disc diffusion susceptibility test by using 10 different antibiotic discs. Molecular investigation was done to detect the virulent gene of salmonella (inv A) and E.coli (eae A) and antibiotic resistant gene for both salmonella and E.coli (blaTEM) using PCR.
Salmonella
E.coli
virulent
resistant
PCR
2021
07
01
12
18
https://bvmj.journals.ekb.eg/article_191379_5c3f895f28cbceae4f8b4b9092c5a480.pdf
Benha Veterinary Medical Journal
1110-6581
1110-6581
2021
40
2
Evaluation the impact of the transition period on some hematobiochemical and hormonal parameters in Native sheep in Algabal Alakhdar governorate in Libya
abdelghany
abdelghany
Almahdi
Akraeim
Salah eldein
Saad
This study aimed to provide a complete picture of selected hematological and serum biochemical parameters in Ewes from late pregnancy to early postpartum period. This study was carried out on 120 clinically healthy Native sheep in Elgabal -Alakhdar province in Libya. Animals were divided into three groups, group1 included 20 non-pregnant ewes, group2- included 50 pregnant ewes and group3 included 50 lactating ewes. Blood samples were collected three weeks before the expected parturition from group 2and during the 1st month post-partum from group3.whole blood samples were collected for hematological picture, while serum samples were collected for biochemical analysis which included, serum calcium, magnesium and phosphorus and serum glucose, AST, ALT, and cortisol. Significant changes between pre- and post-partum period (pregnant- lactating) were observed in values of hemogram and biochemical parameters including RBCs, MCV, WBCS count, and differential leucocytes show significant change in lymphocyte count, glucose, calcium, phosphorus, magnesium, AST, ALT, and cortisol. Non-significant changes were observed in Hb, HCT, Neutrophil, monocyte, eosinophil, platelet and basophil. These results concluded that transition period has an impact on some hematobiochemical parameters and ewes should be supplemented with minerals including calcium, phosphorous and magnesium. Investigations of major and minor elements may be considered as real indicators for health in transition period in ewes and helps to prevent many diseases at this stage.
Hematobiochemicals
Libya
sheep
transition period
2021
07
01
19
23
https://bvmj.journals.ekb.eg/article_191386_dc2917dec80cba54f98a2d4d89a3cbca.pdf
Benha Veterinary Medical Journal
1110-6581
1110-6581
2021
40
2
Evaluation of the microbiological status of some retailed bovine meat and dairy-products with an improvement trial for the beef mince using acetic and lactic acids
Gehan
El tanany
Seham
Elbadry
Hend
Nada
Samah
Abd Ellatif
Heba
El-Sherbiny
Bovine meat and dairy-products are among the important sources for animal-derived protein, vitamins, and minerals. Meat and dairy products can be contaminated during processing, distribution, and storage, and can be implicated in the transmission of many foodborne pathogens worldwide. This study was undertaken to investigate the microbiological status of some bovine meat products (beef mince, sausage, luncheon, and basterma), and some dairy products (raw milk, dried milk powder, yoghurt, and kariesh cheese) retailed in the food markets in in Zagazig city, Egypt. Evaluation of the sanitary status of these products were done via estimation of total bacterial count (TBC), total psychrophilic count (TPsC), coliforms count, total Staphylococci count (TSC) and total mold count (TMC). A trial for improvement of the microbiological status of the beef mince was conducted using acetic and lactic acids at different concentrations. The achieved results indicated unsatisfactory sanitary status of the examined products in the present study, in terms of high microbial counts. A clear and significant reduction for the microbial load was achieved after treatment of the beef mince with acetic and lactic, particularly at 2%.
Beef meat
Dairy products
microbial load
acetic acid
lactic acid
2021
07
01
24
28
https://bvmj.journals.ekb.eg/article_191394_ecd55a2be43abb398dab5f507398ebb4.pdf
Benha Veterinary Medical Journal
1110-6581
1110-6581
2021
40
2
Occurrence of some heavy metals in shellfish: Dietary intakes and health risk assessment
Gehan
El tanany
Shellfish is considered as important source for high quality protein, polyunsaturated fatty acids, vitamins, and minerals. The present study aimed to study the incidence of four heavy metals including lead (Pb), cadmium (Cd), zinc (Zn), and copper (Cu) in four of the shellfish including shrimp, crab, oyster, and mussels. Moreover, the dietary intakes and the potential non-carcinogenic human health risks were calculated for Egyptian consumers. The obtained results revealed that shrimps had the lowest residual concentrations for both Pb, and Cd; whereas mussels had the highest Pb content, while oyster had the highest Cd content. The high content of heavy metals in the examined shellfish indicates the contamination of their living environment with heavy metals. The inter-species differences in their accumulation of heavy metals indicate their physiological differences their xenobiotic metabolizing enzymes. All examined shellfish had considerable concentrations of Cu, and Zn suggesting that these species can provide humans with part of their needs for these essential trace elements. Calculation of the potential non-carcinogenic risks for the tested metals associated with the consumption of shellfish indicated that the average consumption of these shellfish would not pose any risks for the Egyptian population.
Shellfish
Heavy metals
dietary intakes
Health risk assessment
2021
07
01
29
32
https://bvmj.journals.ekb.eg/article_191407_9bea457c0dcaeaf31d9e21092ef9c303.pdf
Benha Veterinary Medical Journal
1110-6581
1110-6581
2021
40
2
Control of some heavy metals contaminating fish products
saad
saad
Shaimaa
Nada
Mahmoud
Nada
mahmoud
nada
One hundred random samples of fesiekh, sardine, smoked herring and canned tuna (25 of each) obtained from different localities in Menoufia governorate, Egypt and estimated for their harmful residues including heavy metals (mercury, lead, cadmium & iron). Additionally, trials to control such serious residues by using biological techniques were applied. It was found that the average values of mercury in the investigated samples of fesiekh , sardine, smoked herring and canned tuna were 1.14 ± 0.02, 0.98 ± 0.01, 0.83 ± 0.01and 0.65 ± 0.01mg/kg respectively and in lead they were 0.54 ± 0.01, 0.42 ± 0.01, 0.29 ± 0.01 and 0.23 ± 0.01mg/kg, respectively. The cadmium residues average concentrations in the examined samples of fesiekh , sardine, smoked herring and canned tuna were 0.33 ± 0.01, 0.21 ± 0.01, 0.16 ± 0.01 and 0.09 ± 0.01mg/kg, respectively while, in iron they were 1.71 ± 0.02, 0.65 ± 0.01, 1.06 ± 0.02 and 0.93 ± 0.01mg/kg, respectively. The effect of L.rhamnosus culture (1x107) on the levels of lead experimentally inoculated to sardine fillets (30 mg/Kg) was decreased to 13.8mg/kg after 8 hours, 7.9 mg/kg after12 hours and 6.5mg/kg after 24 hours by a percentage of reduction 54%,73.7% and 78.3% reduction respectively.
Heavy metals
fish products
Smoked herring
2021
07
01
33
37
https://bvmj.journals.ekb.eg/article_191415_ffea71953cbadb788af1da9855f2d5b8.pdf
Benha Veterinary Medical Journal
1110-6581
1110-6581
2021
40
2
Trials for controlling of biogenic amines in fish products
saad
saad
Shaimaa
Nada
Mahmoud
Nada
One hundred random samples of fesiekh, sardine,smoked herring and canned tuna (25 of each) obtained from various fish markets in Menoufia governorate, Egypt. All collected samples were investigated for their harmful biogenic amines residues (histamine, putrescine cadaverine & tyramine). Additionally, trials to control such serious residues using biological techniques were applied. The mean values of biogenic amines in the examined samples of fesiekh , sardine, smoked herring and canned tuna were 26.48 ± 0.52 , 21.93 ± 0.40 , 18.07 ± 0.29 & 12.61 ± 0.23 mg % for histamine, 17.69 ± 0.31, 14.45 ± 0.26, 12.78 ± 0.22 & 9.10 ± 0.15 mg% for putrescine, 13.56 ± 0.23, 9.81 ± 0.20, 8.93 ± 0.19 & 5.47 ± 0.15 mg% for cadaverine, respectively. On the other hand, the average concentrations of tyramine were 8.92 ± 0.21 mg % for fesiekh, 6.08 ± 0.15mg % for sardine, 3.74 ± 0.14mg % for smoked herring and 2.95 ± 0.09 mg % for canned tuna. The effect of B. polymyxa culture (2x107) on the levels of histamine experimentally inoculated to sardine fillets (40 mg/Kg) was excellent where its level was decreased to 22.1mg/kg after 8 hours, 14.2 mg/kg after12 hours and 8.9mg/kg after 24 hours with reduction percentages of 44.7%, 64.5% and 77.8%, respectively. Keywords: biogenic amines; fish products; histamine.
biogenic amines
fish products
histamine
2021
07
01
38
42
https://bvmj.journals.ekb.eg/article_191417_7ad5dafb1e1bf8245b049db66aa0dcee.pdf
Benha Veterinary Medical Journal
1110-6581
1110-6581
2021
40
2
Prevalence of some bacterial contamination during processing of imported and local natural casing at a plant in Cairo governorate
Lamia
Fahmy
Hemmat
Ibrahim
Reham
Amin
Mostafa
Hassouba
A total of 90 random samples represented by local and imported natural sheep Casing (45 of each) were collected during certain processing steps from a plant which is working in processing natural casing and implementing HACCP system at Cairo Governorate .These samples subjected to some bacteriological examination as Aerobic Plate Count (APC), Enterobacteriaceae, Coliform Count, Staphylococci count .The results revealed that imported samples showed relatively higher mean values than the local one; where APC was (5.47± 0.20,5.51± 0.18, 5.56± 0.22) log CFU/g ,Enterobacteriaceae was (1.41± 0.14 ,1.99± 0.23, 1.81± 0.27) log CFU/g, Coliform was (1.42±0.22,0.98± 0.21,0.92± 0.26) log CFU/g and Staphylococci was ( 3.16±.0.17,3.21±.0.15, 3.16±.0.12) log CFU/g for imported samples at intial Salting , Sorting, Final Salting Steps; respectively. While, APC was (5.48± 0.13,5.62± 0.14, 5.47± 0.12) log CFU/g ,Enterobacteriaceae was (1.00± 0.00 ,1.95± 0.19, 1.23± 0.12) log CFU/g, Coliform was (0.83±0.13,1.25± 0.21,0.54± 0.07) log CFU/g and Staphylococci was ( 3.09±.0.17,3.06±.0.15, 2.71±.0.16) log CFU/g for local samples at intial Salting , Sorting, Final Salting Steps; respectively.
sheep casing
Enterobacteriaceae
Staphylococci
coliforms
2021
07
01
43
47
https://bvmj.journals.ekb.eg/article_193252_1bc240254f2a3e2dfbc2408c6a10f7b4.pdf
Benha Veterinary Medical Journal
1110-6581
1110-6581
2021
40
2
Effect of marjoram oil on the quality and shelf life of meat
A.M
Edris
Shimaa N.
Edris
abila I.
El Sheikh
Tereza
Amin
Essential oils can improve shelf life of meat due to its antimicrobial and antioxidant action. This study aimed to clarify the effect of marjoram essential oil (MEO) at different concentrations (0.5%, 1% & 1.5%) on meat fillet (250 gm each) which divided into four groups one untreated group (control) and three treated groups with MEO. All samples are examined for sensory properties and keeping quality criteria (pH, TVN &TBA) during cold storage at 40C for 15 days. The obtained results significantly showed lowering values (P<0.05) for sensory and chemical assessment than untreated (control) one. In addition MEO treated samples with 1.5% concentration had the greatest impact which revealed that mean values of PH,TVN(mg%) and TBA(mg /kg) at the end of the trial where 6.19,18.64 and 0.81 &6.43,19.9 and 0.89 for samples treated with 1% marjoram oil concentration and6.48 , 20.68 and 0.93 for samples treated with 0.5% marjoram oil concentration. Therefore, marjoram oil with special reference to 1.5% concentration could be used as an alternative option to synthetic chemical substances to improve sensory and chemical properties as well as extending shelf life of meat fillet during cold storage.
meat
marjoram
pH
TVN
tba
2021
07
01
48
52
https://bvmj.journals.ekb.eg/article_193262_3ff8eece09851d9704d514a31daa9ca1.pdf
Benha Veterinary Medical Journal
1110-6581
1110-6581
2021
40
2
Chemical evaluation of imported fish
Saad
M.S.
Islam
I.S.
Islam
Z
ibrahim
ahmed
Canned fish portions had laid down limits of pH that should not be more than 6.7; TVN should not be more than 40 mg/l00g (EOS, 2005) .This study was conducted on 105 samples of imported canned fish for recording the chemical evaluation of these samples. The chemical examination declared that the mean values of TBA and TVN were 14.8±1.4 and 23±1.5 mg% for canned mackerel, 4.8±0.4 and 23.2±0.6 mg% for canned sardine and 1.9±0.1 and 18.6±2.5 mg% for canned tuna, respectively. Significant differences (P≥0.05) appeared between the examined samples of canned mackerel, sardine and tuna as a result of TBA and TVN values. current study classified the presence of some unacceptable imported canned fish for human consumption .that indicate the importance of chemical evaluation of canned fish. recommended future study must be applied chemical and molecular methods to compare efficiency of current chemical method to control this problems in future and save people health .
TVN
tba
canned fish
2021
07
01
53
55
https://bvmj.journals.ekb.eg/article_193277_8353d998aa0cae8bc73387468ca7c31e.pdf
Benha Veterinary Medical Journal
1110-6581
1110-6581
2021
40
2
Bacteriological evaluation of imported canned fish with special reference to Clostridium perfringens .
Saad
M.S
Islam
I.S
Islam
z.
ibrahim
ahmed
Anaerobic and facultative anaerobic microorganisms are important group of microorganisms responsible for many health hazards to consumer of canned food.This study was conducted to bacteriologically evaluating 105 sample of imported canned fish from different markets at kaluobia governorate . Bacteriological examination indicated that the mean values of the examined samples of canned mackerel, sardine and tuna were 1.7 x104±1.1 x104, 1.4 x103±5 x102&1.7 x103±9.2 x102/ g for total anaerobic counts and 1.1 x103±5.7 x102, 7.5 x102±2.7 x102&1.9 x103±1.5 x103/g for total C. perfringens counts, respectively. Moreover, C. perfringens was detected in 25.7%, 14.3% and 11.4% of the examined canned mackerel, sardine and tuna samples, respectively. However, C. bifermentans, C.subterminal, C. sporogenes, C. sordelli were isolated from such examined samples of canned fishes at different percentages .The current study classified the presence of unacceptable canned imported fish for human consumption so recommended future studies must using advanced methods to control this problems in The future and save people health.
canned fish
clostridia
Bacteriological
2021
07
01
56
60
https://bvmj.journals.ekb.eg/article_193284_8bc8d74cb1ab885f7c2edb1c16b9c20d.pdf
Benha Veterinary Medical Journal
1110-6581
1110-6581
2021
40
2
Quality assessment of yoghurt produced for foreign markets
doaa
elhashala
Hamdi
Mohammed
marwa
Saad
One hundred and forty random samples, 20 samples of plain yoghurt produced for foreign markets(F), 60 samples of plain yoghurt produced for local markets20 of each brand plain yoghurt (A, B, C) 60 samples of fruit yoghurt produced for local markets 20 for each brand (M, N, L) were collected from the marketing points. The collected samples were examined organoleptically, chemically, and microbiologically. The obtained results revealed that the highest score of examined yoghurt samples for sensory evaluation was F brand of examined plain yoghurt samples than fruit yoghurt samples (M, N, L). Acidity% in plain yoghurt samples were 0.90, 0.95, 1.06 and 0.98(F, A, B, C) respectively, while in case of fruit yoghurt samples were 0.92, 1.03 and 0.91(M, N, L) respectively. The mean coliform count/g in examined yoghurt samples was 1.85±1.01, 1.54 x102±0.07 x102 for plain yoghurt (B, C) while, 1.62 x10±1.12 x10 in fruit yoghurt (M).The mean staphylococci count/g in examined natural yoghurt were (F, A, B,C) 0, 1.45x10±0.95x10C, 8.85x10±8.01x10 and1.54 x102±1.02x102 ,respectively. While in fruit yoghurt (M, N, L) the mean values were 1.62X102 ± 1.12 X 102, 9.25X10 ±8.01X10 and5.54 X 10 ± 4.01 X 10.
Yogurt
Milk
Assessment
2021
07
01
61
66
https://bvmj.journals.ekb.eg/article_193290_8eb8499bc417b8df4349a8e83b875157.pdf
Benha Veterinary Medical Journal
1110-6581
1110-6581
2021
40
2
Occurrence of multidrug resistant shiga-toxigenic E. coli in retailed cheese in Zagazig city, Egypt
Samah
Abd Ellatif
Seham
Elbadry
Hend
Nada
Lamiaa
Reda
Asmaa
Tahoun
Cheese is regarded as an essential dairy product that cover part of the human needs with vitamins and minerals. However, cheese is also implicated in many food poisonings outbreaks worldwide. This study conducted to estimate coliforms and E. coli, and to investigate the occurrence of shigatoxigenic E. coli in four cheese types mostly consumed in Zagazig city, Egypt. In addition, detection of coding genes for shiga toxins (stx1, and stx2) was further screened using PCR. Besides, antimicrobial susceptibility of the isolated E. coli was further examined. The obtained results revealed that Kariesh cheese had the highest coliforms and E. coli counts, followed by Rumy, Domiati, and Feta cheeses, respectively. The isolation rates of E. coli were 52%, 24%, 20%, and 8% in Kariesh, Rumy, Domiati and Feta cheeses, respectively. The identified E. coli serotypes were E. coli O55:H7, E. coli O86:H11, E. coli O78:H-, E. coli O111:H4, E. coli O127:H6, and E. coli O26:H11. The expression of shiga toxin-coding genes indicated that E. coli O55:H7 harbored only stx1, E. coli O111:H4, and E. coli O86:H11 harbored only stx2, E. coli O78:H-, and E. coli O26:H11 harbored both stx1, and stx2; however, E. coli O127:H6 did not express any of the tested genes. Antimicrobial sensitivity testing revealed multidrug resistance profiles, particularly among E. coli O86:H11, E. coli O78:H-, and E. coli O26:H11. Therefore, strict hygienic measures should be adopted during all manufacture steps of these kinds of cheese.
E. coli
cheese
Egypt
Antimicrobial sensitivity testing
Shiga toxins
2021
07
01
67
71
https://bvmj.journals.ekb.eg/article_193292_3925f6794d00f15f5f53c9aada281af4.pdf
Benha Veterinary Medical Journal
1110-6581
1110-6581
2021
40
2
Case Report: Bronchiolitis Obliterans in Dromedary camel (one-humped) in Aswan Slaughter houses, Egypt
marawah
ahmed
Respiratory disorders in camels are very common and often cannot be easily identified in live animals until complicated. Thus, the only way to understand the pathology behind respiratory disorders and their prompt diagnosis is by examining lungs at slaughter. So, this study was designed to describe bronchiolitis obliterans in camel when reported as a case report. Bronchiolitis obliterans is a form of rare pulmonary disease. It happens when tiny airways in the lungs, called bronchioles, are scarred. This scarring makes it difficult for the air to flow and for the animals to breathe, causing the airways to narrow. Lung biopsy can be the best way to diagnosis bronchiolitis obliterans. Thus, during post-slaughter inspection of dromedary camels (Camelus dromedaries) slaughtered at the Draw Abattoir, Aswan, Egypt, one case of bronchiolitis obliterans in adult camel was reported. Histopathological features revealed intraluminal polyps of inflammatory granulated tissues that obstruct small airways. In addition, regions of emphysema were located in the neighboring alveoli and some of them were packed with exudate. Finally, it can be concluded that bronchiolitis obliterans occur in one humped camel.
Case Report
bronchiolitis obliterans
histopathology
2021
07
01
72
74
https://bvmj.journals.ekb.eg/article_193293_c863a479b25653e50e3117a5ef6b755e.pdf
Benha Veterinary Medical Journal
1110-6581
1110-6581
2021
40
2
Clinicopathological Evaluation of Anti-inflammatory and Hepatoprotective Effects of Rutin in Guinea pigs
warda
mohamed
Ali
Al-baddany
Esam
Aqlan
This study was carried out to evaluate the anti-inflammatory activity of flavonoid (Rutin 20 mg/kg) on each (carrageenan-induced paw edema)acute inflammation and (cotton pellet granuloma) chronic inflammation and, effect of Rutin on some hematological, biochemical and histopathological examination in guinea pigs induced by inflammation. A total of eighteen male guinea pigs were categorized into three groups, six pigs on all group. Group one as non-treated control, they given 5% carboxymethyl cellulose orally for seven days. Group two received single dose orally (20mg/kg) Rutin for seven days. Group three received (10mg/kg) Indomethacin orally for seven days. By injecting of 0.1ml of carrageenan suspension in saline into the sub-planter superficies of the right hind paw, the acute inflammation (edema) was induced in all experiment guinea pigs one hour after compounds taken. Then estimated the volume of paw edema immediately after carrageenan injection through one, two, three and four hours by using a plethysmograph system(VGO Basile, Italy). The results indicated that, Rutin (20 mg/kg body weight) showed significant lowered (P<0.05) in the carrageenan induced paw edema and cotton pellet induced granuloma model comparing with control and indomethacin groups. In addition Rutin (20 mg/kg) improved the hematological and biochemical profiles and able of maintain the biochemical varies related to inflammation. This was also supported by histopathological studies of liver sections. On conclusion, Rutin possess anti-inflammatory activity. Thus the Rutin showed protective role against acute and chronic inflammation.
anti-inflammatory activity
Rutin
Carrageenan
cotton pellet granuloma
histopathology
2021
09
06
75
80
https://bvmj.journals.ekb.eg/article_193294_1d5b0790261111821947c3dfbd4d652e.pdf
Benha Veterinary Medical Journal
1110-6581
1110-6581
2021
40
2
Potential Reno-protective and Anti-inflammatory Effects of Rutin in Guinea Pig
warda
mohamed
This work was carried out to evaluation the Reno-protective effect and anti-inflammatory activity of rutin in guinea pigs. Twenty four male guinea pigs categorized into 4 groups, six pigs in each group. Group one as a normal control given (2ml/kg)distilled water. Second group as non-treated control were given 5% of carboxymethyl cellulose orally. Third group, animals were received Rutin (20mg/kg). The fourth group were given aqueous suspensions of Indomethacin (10mg/kg) in 5% Polysorbate 80 orally once daily for seven days. For model of chronic inflammation each pig were anesthetized by light ether and then after thirty minutes a sterile cotton pellet weighing (50 mg±1) was implanted under the skin on both sides for the scapular region. All pigs were anaesthetized by using chloroform on the 8th day then the blood collected. They were dissected kidney and put it in 10% formalin for histological test. Then isolated the cotton pellets from each, dried at 60Co and estimated the dry weight. Administration of rutin (20mg/kg body weight) for seven days showed significant(P<0.05) inflammation inhibition which percent of inhibition were 33% for(Rutin), 37% for (Indomethacin) and 0% for control. Also, Rutin (20 mg/kg) improved the biochemical related to inflammation. This was also supported by histopathological findings of the examined kidneys. On conclusion, Rutin possess anti-inflammatory activity and protective role against granuloma-induced alterations.
anti-inflammatory activity
Rutin
cotton pellet granuloma
histopathology
2021
07
01
81
85
https://bvmj.journals.ekb.eg/article_193295_18c3bc8254bb7e24e21a08a2038f3137.pdf
Benha Veterinary Medical Journal
1110-6581
1110-6581
2021
40
2
Beneficial effects of Omega-3 polyunsaturated fatty acids in treatment of non-alcoholic fatty liver disease in rats.
Samy
Hussein
Yakout
Elsenosi
Mohamed
Khalid
Marwa
Fawzy Atta
Nonalcoholic fatty liver disease (NAFLD) is sickness with different causes and it is the most hepatic illness. The objective of this work is to investigate the bimolecular changes in a model of high fat diet induced- NAFLD in rats. Also, the hypolipidemic, antioxidant and anti-inflammatory effects of omega-3 were clarify via evaluation of some biochemical and molecular parameters in the liver tissue of rats.Twenty one adult white albino rats were divided into three groups. Group I (Normal control): received no drugs and fed balanced diet, Group II (NAFLD- induced group): rats fed high fat diet for 8 weeks followed by normal basal ration for additional 8 weeks. Group III (NAFLD + omega-3): rats administrated omega-3 (300 mg/kg b.wt/day), orally for 8 weeks post-induction of NAFLD. The obtained results showed a significant increase in serum Total cholesterol and Triacylglycerols (TAG) concentrations, L-MDA and TAC in liver tissuewith significant up-regulation in CPT-1α, FAS, SREBP-1c, PPAR-α, Nrf2 and TAK1gene expression in NAFLD-induced rats. Treatment with Omega-3 to NAFLD induced rats potentially improved molecular hepatic cell function and oxidative alterations related to NAFLD near its normal ranges. Meanwhile, histo-pathological findings supported that Omeg-3 markedly attenuates harmful effects of NAFLD and protected liver cell. On conclusion, Omega-3 plays an important role as potent anti-oxidant, anti-obesity, anti-inflammatory via inhibition of hepatic steatosis and oxidative stress singling.
NAFLD
omega-3
Oxidative Stress
inflammatory biomarkers
histopathology
2021
07
01
86
90
https://bvmj.journals.ekb.eg/article_193296_54b9d6931a11df463994db360c7687dc.pdf
Benha Veterinary Medical Journal
1110-6581
1110-6581
2021
40
2
Ameliorative effect of Mg-Salvia Officinalis Nanoparticles against Aluminum Chloride induced oxidative stress in rat model of Alzheimer's Disease
Eman
Elkomy
omayma
abouzaid
Mohamed
Mahfouz
Afaf
Desouky
Alzheimer’s disease (AD) which is usually referred to as Alzheimer’s is one of the popular reasons for dementia. Mg-Salvia Officinalis NPs has been known as an interesting compound with antioxidant, anti-inflammatory and cognitive properties. The possible therapeutic effect of Mg-Salvia Officinalis NPs was evaluated in Aluminum Chloride (ALCL3) induced AD. AD was induced by intraperitoneal injection of ALCL3 at a dose (100 mg/kg b. wt.) for 2 weeks. Fifty male rats were equally divided into 5 groups. Group I (Normal control): Rats received no drugs, Group II (ALCL3- induced AD): Rats injected with ALCL3 (100mg/kg b. wt./I. P) for two weeks, Group III (Mg-Salvia Officinalis NPs treated): After 14 days of ALCL3 injection, rats treated with Mg-Salvia Officinalis NPs (5mg/kg b. wt./day, orally) for four weeks. Group IV (Donepezil treated): Rats injected with ALCL3 as group II and treated with Donepezil (1mg/kg b. wt./day, orally) for four weeks. Group V (Mg-Salvia Officinalis NPs and Donepezil treated): Rats injected with ALCL3 and treated daily with Mg-Salvia Officinalis NPs and Donepezil for four weeks. The results revealed that ALCL3-induced AD rats causing significant alterations in Tau protein and acetylcholine levels in brain tissue in addition to marked elevation of serum ALT, AST activities, urea, creatinine concentrations and oxidative stress biomarkers. Treatment with Mg-Salvia Officinalis NPs to AD rats caused marked improvement effect in all previous parameters. Conclusively, Salvia Officinalis NPs treatment ameliorates oxidative stress induced by ALCL3 in rat’s model of AD and enhances antioxidant defense system, and prevent the lipid peroxidation.
Alzheimer’s disease
Salvia officinalis
tau protein
ACH
antioxidants
2021
07
01
91
95
https://bvmj.journals.ekb.eg/article_193297_42829e6ec968b87ad52ed08e642a491a.pdf
Benha Veterinary Medical Journal
1110-6581
1110-6581
2021
40
2
Calories restriction modulates the enzymatic antioxidant status in D-galactose induced aging in male rats
Mohammed
Mohammed
omayma
Abo zaid
Fatma
Moawed
Omama
Elshawi
In postindustrial and modern societies, overeating and high calories consumption has emerged as new challenge in community health. Large effort is now being devoted to detect and evaluate the pathophysiologic consequences of aging and aging accompanying diseases. Several lines of evidence propose that caloric intake influences the speed of aging and the onset of linked diseases in animals and, possibly, humans. These are the clues of decreasing calories intake in this study to evaluate the effect on aging and to detect weather there are ways to control it. Thirty adult male albino rats were used in this work. ten rats as the control group, D-galactose was injected intraperitoneally to induce aging, then rats were sub divided into equal two groups, one group subjected to calories reduction while-the-other-took-normal-diet-.Catalase,-glutathione-and-MDA-were carried out to test the oxidative effect of D-galactose and possible protective effect of treatments. D-galactose induced impaired oxidative functions. Calories reduction improved D-galactose induced deterioration to a great extent as evidenced biochemically.
aging
anti-oxidants
calorie restriction
D-galactose
2021
07
01
96
99
https://bvmj.journals.ekb.eg/article_193298_ffefb4f18dcf8aab9dc6843891b7796e.pdf
Benha Veterinary Medical Journal
1110-6581
1110-6581
2021
40
2
Therapeutic and protective effect of selenium nanoparticles against experimentally induced hepatotoxicity in rats
Afaf
Desouky Abdel Magid
Hussien
Abdelmaksoud Ali
Marwa
Ahmed
Selenium nanoparticles have received wide attention because of their importance in nutrition compared to other forms of selenium used in food fortification. Within recent study, selenium nanoparticles (SeNPs) were prepared, characterized and evaluated in albino rats to identify their hepatic protective effect against cyclophosphamide-induced hepatotoxicity. Fifty white albino rats were divided randomly into five groups. Group I was used as a negative control, group II was administrated daily dose of CP (5 mg/kg body weight/8 weeks) orally by gavage, group III were administrated SeNPs orally at a dose of (2 mg/kg body weight / 12 weeks) three times weekly. Group IV was administrated CP at a dose of (5 mg/kg body weight/8 weeks) daily orally then administrated SeNPs orally at a dose of (2 mg/kg body weight / 4 weeks) three times weekly. Group V(protected group) was administrated SeNPs orally at a dose of (2 mg/kg body weight/4 weeks) three times weekly, then administrated daily dose of CP (5 mg/kg body weight/8 weeks) orally with continuous administration of SeNPs till the end. Based on results obtained, CP administration in group II showed significant increase in serum liver enzymes activity(AST, ALT, and ALP), which was followed by a substantial significant decrease in serum albumin conc. and tissue antioxidants (GPx, SOD, and CAT) activity, with significant increase in cytokine levels (IL6,IL1β). Such biochemical changes were significantly improved by Nano-Se treatment, particularly in the protected rats. The current study found that Nano-Se reduces oxidative stress in liver tissue caused by CP administration.
Hepatotoxicity
nano selenium
cyclophosphamide
Antioxidant enzymes
Cytokines
2021
07
01
100
104
https://bvmj.journals.ekb.eg/article_193300_d0b08eeafaf754ddf2269ee4edacd0b9.pdf
Benha Veterinary Medical Journal
1110-6581
1110-6581
2021
40
2
Therapeutic and protective role of curcumin nanoparticles against experimentally induced hepatotoxicity in rats
Marwa
Ahmed
Afaf Desouky
Abdel Magid
Hussien Abdelmaksoud
Ali
The goal of this research was to see if using Cur-NPs could boost curcumin bioavailability and to see whether Cur-NPs could protect rats from Cp-induced hepatotoxicity. Five groups of fifty white albino rats were formed at random. Group I acted as negative control while group II received Cyclophosphamide daily dosage of (5 mg/kg body weight) via oral gavage for 8 weeks. Group III received Cur-NPs at a dosage of (20 mg/kg bw) 3 days a week via oral gavage for 4 weeks. Group IV received CP as group II and treated with Cur-NPs as group III for 4 weeks. Group V received Cur-NPs as protector, for 4 weeks before CP administration as group II with continuous administration with Cur-NPs till the end.At the end of the experiment, blood and tissue samples were obtained for biochemical, antioxidant enzyme, and cytokine level determination. According to the findings, giving CP to rats in group II increased serum liver function enzymes (ALT,AST, and ALP), which was followed by a substantial decrease in serum albumin conc. and tissue antioxidants (GPx, SOD, and CAT) activity, as well as increased levels of cytokines (IL-6, IL-1β). Cur-NPs treatment improved these biochemical changes dramatically, particularly in group V.These findings suggest that Cur-NPs may be a potential hepatoprotective agent for reducing liver oxidative stress caused by various stress factors.
Hepatotoxicity
Nano curcumin
cyclophosphamide
Antioxidant enzymes and Cytokiens
2021
07
01
105
110
https://bvmj.journals.ekb.eg/article_193319_bf9ce0961c793690980dc33dc65e0137.pdf
Benha Veterinary Medical Journal
1110-6581
1110-6581
2021
40
2
Role of Asparagus racemosus root in the amelioration of hepatocellular carcinoma in rat"
Yakout, A.
El-senosi
Samy
Aziza
Sawsan
Elsonbaty
Mohamed
Abo Alella
The purpose of this study was to look into the antitumor activity of Asparagus racemosusroot (AR) against hepatocellular carcinoma (HCC) in male rats that had been induced experimentally. Fifty white albino rats were categorized randomlyto five groups. Group I act as a negative monitor.Group II was given DEN by gavage at dose of (20 mg/kg b.w/day/12 weeks). Group III rats were given AR (200 mg/kg b.w/18 weeks) via gavage. Group IV rats were given DEN at dose of (20 mg/kg b.w/day/12 weeks and then ARat dose of 200 mg/kg b.w/ 6 weeks. Group V rats were given AR at dose of(200 mg/kg b.w / 6 weeks before administration of DEN dose of (20 mg/kg b.w/day/12 weeks) till end ofexperiment.According to the findings, DEN treatment increased serum liver function enzymes ALT, AST, and ALP while decreasing the activity of hepatic antioxidant enzymes (SOD and CAT). DEN raised IL-6 levels while decreasing the apoptotic marker p53. Asparagus supplementation prior to or after DEN administration significantly reduced DEN toxicity and mutagenic effects, particularly when used as a protective. According to the findings, Asparagus racemosusroots should be used as an antioxidant and antitumor.The aim of this study was to seeif amedicinal plant(AR) could prevent rats from developing hepatocarcinogenesis caused by diethylnitrosamine (DEN).
Hepatocellular carcinoma
Asparagus racemosus
p53
SOD
CAT
2021
07
01
111
116
https://bvmj.journals.ekb.eg/article_193302_69bced377f46e0539127b0b5d536d92d.pdf
Benha Veterinary Medical Journal
1110-6581
1110-6581
2021
40
2
The protective effects of Spirulina against Nonalcoholic fatty liver disease induced by high fat diet in rats
Samy
Hussein
Yakout
Elsenosi
Mohamed
Khalid
Marwa
Fawzy Atta
The ultimate goal of this study is to investigate the bimolecular changes in a rat model of high fat diet induced Nonalcoholic fatty liver disease (NAFLD). Also, the lipotropic, antioxidant and anti-inflammatory effects of spirulina platensis treatment were clarify through evaluation of certain biochemical and molecular parameters in the blood and liver tissue of rats. Twenty one adult white albino rats were separated into three groups. Group I (Normal control): received no drugs and balanced diet, Group II (NAFLD- induced group): rats fed high fat diet for 8 weeks followed by normal basal ration feeding for another 8 weeks. Group III (NAFLD + spirulina): rats treated with spirulina orally at a dose of (150 mg/kg b.wt/day) for 8 weeks after induction of NAFLD. The obtained results showed a significant increase in serum total cholesterol (TC) and triacylglycerols (TAG) concentrations with significant up-regulation of liver gene expression TNF-α, IL-1β, NF-kβ, PPAR-α, ACC, FAS and SREBT-1in NAFLD- induced rats. Also, various histopathological alterations were detected in liver tissue of induced NAFLD rats. Treatment with spirulina in NAFLD-induced rats parameters measured near the normal level of rats group (G1). Also, spirulina potentially improved molecular hepatic function alterations related to NAFLD. Interestingly, histopathological findings supported that spirulina markedly attenuates harmful effects of NAFLD and protects liver tissue. We conclude that, spirulina has a great role as antioxidant, anti-obesity and anti-inflammatory via inhibition of liver lipid metabolic disturbance, steatosis and oxidative stress singling.
Nonalcoholic fatty liver disease
Spirulina
Oxidative Stress
Inflammatory mediators
histopathology
2021
07
01
117
120
https://bvmj.journals.ekb.eg/article_193303_23b4386b44980beaf3945b914f67da88.pdf
Benha Veterinary Medical Journal
1110-6581
1110-6581
2021
40
2
Protective and therapeutic role of nano-curcumin against hepatocarcinogenesis in rat
Yakout, A.
El-senos
Samy
Aziza
Sawsan
Elsonbaty
Mohamed
Abo Alella
Curcumin medicinal applications are hindered by its well-known unstable metabolic state and also insufficient absorption and bioavailability.In recent years, nano-based drug delivery system has effectively improved the aqueous solubility and bioavailability of curcumin. The current study was established to evaluate the protective and therapeutic effect of nano-curcumin (Nano-Cur) in hepatocellular carcinoma induced in rats. Fifty white albino rats were divided into 5 groups 10 rat of each. Group I served as negative control while group II received DEN with a daily dosage of (20 mg/kg body weight) orally by gavage during twelve weeks. Group III rats received Nano-Cur (20 mg/kg body weight) three days a week by gavage for eighteen weeks. Group IV rats received DEN as group II and treated with Nano-Cur as group III for six weeks. Group V received Nano-Cur as protector, for six weeks before DEN administration with continuous administration with Nano-Cur till the experiment end. Blood and tissue samples were collected and removed for determination of biochemical, antioxidant, cytokine and apoptotic marker p53 examination at the end of experiment. Based on the findings, DEN group indicated significantly increased serum liver function enzymes ALT, AST, ALP this was accompanied with significant decrease in liver antioxidant enzyme activities of (SOD and CAT) along with significant elevation in lipid peroxidation. DEN significantly elevated IL-6 and reduced apoptotic marker p53. The findings of this research indicated that Nano-cur has a anemaelioration effect against DEN carcinogenic effect.
Hepatocellular carcinoma
Nano curcumin
catalase
p53
IL-6
2021
07
01
121
125
https://bvmj.journals.ekb.eg/article_193301_a5c12beb384c6374a50d4a5c9345b764.pdf
Benha Veterinary Medical Journal
1110-6581
1110-6581
2021
40
2
Molecular characterization and isolation of Infectious laryngotracheitis virus (ILTV) strains causing outbreaks in layer chicken farms of Kalubia Province, Egypt
Shaimaa
Farag
Kamel
zyan
Ahmed
Eissa
Infectious Laryngotracheitis (ILT) is an acute highly contagious respiratory disease of chickens. It has significant economic importance due to mortalities and the drop in egg production. In this study, seventy samples from different layer farms were collected from the outbreaks that occurred in Kalubia province, Egypt, at the period from February 2018 till May 2019 to detect ILTV by molecular characterization through polymerase chain reaction assay (PCR) as well as isolation on the specific pathogen free- embryonated chicken eggs (SPF-ECEs) through chorioallantoic membrane (CAM) route. Post mortem examination of infected chickens revealed hemorrhagic tracheitis with fibrino-hemorrhagic casts and caseated materials. The PCR revealed amplification of a 688bp fragment of Infected Cell Protein 4 (ICP4) gene. Following that, seven samples were sequenced and phylogenetically analyzed. Sequence analysis of the ICP4 gene of these samples revealed complete identity with TCO (tissue culture origin) vaccines and the vaccine-related strains, which were previously isolated from Giza, Sharkia, Kafrelsheikh, and Fayoum provinces through the years 2007 to 2018. Inoculation of ILTV PCR positive samples on SPF-ECE appeared as yellowish-white pock lesions on the inoculated CAM from the first passage. From these results, we can say that ILTV circulating in Egypt is a vaccinal strain that regains its virulence from the back passage in birds and causes outbreaks all over the country.
ILTV
Egypt
PCR
ICP4
CAM
2021
07
01
126
130
https://bvmj.journals.ekb.eg/article_193340_3fb106ee319f5f7dd32756d767d2e459.pdf
Benha Veterinary Medical Journal
1110-6581
1110-6581
2021
40
2
Coccidiosis in Japanese quails (Coturnix coturnix japonica) in Kalioubia governorate: prevalence and treatment trials
Mohamed
Ramadan
Reham
Elmadawy
Ibrahim
Tolba
This study was undertaken to identify Eimeria species in Japanese quail (Coturnix coturnix japonica) from different localities in Kalioubia governorate. In addition, studing the efficacy of some natural anticoccidial products (Propolis and Neem; Azadirachta indica) compared to Amprol (amprolium hydrochlorid.plus ethopobate) as chemical anti coccidial drug on the oocyst output, pathogenicity (weight gain, feed conversion ratio, mortality, severity of diarrhea, and intestinal lesion scores and oocysts sporulation. Out of 900 examined quails, 575 (63.8%) were infected by Eimeria. Quail farms in Abozabl showed the highest infection (73.3%), while the lowest infection (58.6%) was recorded in quail farm in Kafrshokr. The detected species were E. bateri, E.uzura, E. tsunodai, , Eimeria bahli and Eimeria colini. On the basis of the study results, it was concluded that Amprol showed better results in term of reduction of oocyst count and reduction of sporulation%, followed by Propolis and Neem. All the used drug had a good effect on weight gain, feed consumption. In addition, decreasing the coccidial pathogenicity and mortalities.
Japanese quail
Eimeria
Amprol
Propolis
Neem
2021
07
01
131
136
https://bvmj.journals.ekb.eg/article_193341_a6912ec90e79e87438d6a84f505e3219.pdf
Benha Veterinary Medical Journal
1110-6581
1110-6581
2021
40
2
Effect of dietary supplementation of phytogenic feed additives on broiler performance and economic efficiency
Nasser
Khader
tahia
ahmed
Aya
Ahmed
This study was conducted to explore the use of phytogenic feed additives (PFAs) in broiler nutrition as a natural growth promoter. Two commercial phytogenic products; Activo ® and Digestarom® P.E.P were added to a standard diet to determine their effect on growth performance and cost economics. A total of 180 one-day-old chicks (Cobb 500) were allotted to three treatment groups of three replicates (20 chicks / replicate). The dietary treatments were as follows: group (1) control (basal diet), group (2) basal diet + 100g/ton Activo® and group (3) basal diet + 150g/ton Digestarom® . Results showed that chicks fed PFAs had significantly higher (P ≤ 0.05) body weight and body weight gain than control, however feed intake was decreased (P ≤ 0.05) in supplemented groups than control and, in turn, caused an improvement (P ≤ 0.05) in feed conversion ratio. European Broiler index was increased in Activo® fed group and Digestarom® fed group compared to negative control. Interestingly, a lower feed cost was recorded in the supplemented groups combined with a higher growth rate and, therefore, a higher net return was observed. Moreover, economic efficiency showed a significant (P ≤ 0.05) difference in PFAs supplemented groups compared to the control .In conclusion, supplementation of broiler diets with PFAs can improve body weight gain, feed conversion ratio and overall broiler performance .The best economic efficiency was recorded in broilers fed PFAs.
Phytogenic feed additives
broiler
Growth performance
Cost economics
2021
07
01
137
143
https://bvmj.journals.ekb.eg/article_193361_84a5e63e03f356723d54c0b205f44075.pdf
Benha Veterinary Medical Journal
1110-6581
1110-6581
2021
40
2
Alleviation of imidacloprid-induced oxidative stress and immune damage by Spirulina platensis in broiler chickens
Amira
Abotaleb
Mohamed
AboSalem
Elham
Elshewy
Ahmed
Abdeen
Imidacloprid (IM) is a neonicotinoid insecticide being used extensively for crop protection and pet flea control programmes. However, IM is also a strong environmental toxicant for both animals and humans. As a result, the present study aimed to assess the ameliorative efficacy of Spirulina platensis (SP) aganist IM-induced oxidative stress and immune damage in broiler chickens. Chicks divided randomly into six equal groups (17 each). Group I administered filtered water orally for 4 weeks. Group II administered IM 10 mg/kg b.wt orally daily for 4 weeks. Group III given Spirulina 5 g/kg of diet for 4 weeks. Group IV received IM plus Spirulina for 4 weeks. Group V received IM for 2 weeks, followed by Spirulina for another 2 weeks. Group VI received spirulina for 2 weeks, followed by IM for another 2 weeks. Results revealed that IM significantly decreased body weight while increased feed conversion rate (FCR), total leukocytic count (TLC) and lymphocyte percent. Serum showed a decrease in haemagglutination inhibition (HI) antibody titer against newcastle disease (ND) vaccine. Antioxidant enzymes such as reduced glutathione (GSH) and catalase (CAT) significantly decreased; while malondialdehyde (MDA) increased in bursa of Fabricius tissue accompanied with histological changes. Administration of Spirulina in combination, before and after treatment with IM significantly ameliorated the IM-induced oxidative stress and immune damage due to its antioxidant properties.
Imidacloprid
Spirulina platensis
Oxidative Stress
Immunotoxicity
2021
09
08
144
148
https://bvmj.journals.ekb.eg/article_193638_1b8f7f77b5fb98fdcace42bd958e923e.pdf
Benha Veterinary Medical Journal
1110-6581
1110-6581
2021
40
2
Phenotypic and genotypic studies on antibiotic resistant Yersinia enterocolitica isolated from milk and milk products in Kaliobia, Egypt
Ahmed
Abdelwahab
Ashraf
Abd El-Tawab
Fatma
Abdallah
Ahmed
Maarouf
The study was applied on 175 random samples of milk and milk products collected from different shops (35 of each), at Kaliobia governorate, Egypt, for detection the propagation of Y. enterocoliticastrains in these samples, beside the phenotypic characterization and detection of some antibiotic resistant virulence genes in them.The results gained from these samples by bacteriological examination represent19 (10.9%) Y. enterocolitica biotypes 1Aand 1B only isolates from 175 samples, from milk and Kareish cheese samples (5/14.3% for each ),(4/11.4%)white cheese,(3/8.6%) ice cream, and(2/5.7%) from yoghurt samples.The antibiotic sensitivity profile showed that, the isolated Y. enterocoliticawere very high resistant for Penicillin-G followed by methicillin, ampicillin,oxytetracycline,amoxicillin, ampicillin, streptomycin and erythromycin. Meanwhile, they were highly sensitive to meropenem and norfloxacinfollowed by gentamycin,ciprofloxacin, cefotaximeand florphenicol. PCR declared thatblaTEM andtetAgenes were detected in all eight studied Y. enterocoliticaisolates. So, it was concluded that,the presence of antimicrobial resistant Y. enterocolitica strains in dairy products could be a public health concern for the consumers.
milk products
Yersinia enterocolitica
antibiotic resistant genes
2021
07
01
149
153
https://bvmj.journals.ekb.eg/article_193628_de7574e24937bf87a7f438f09b96a2ec.pdf
Benha Veterinary Medical Journal
1110-6581
1110-6581
2021
40
2
Bacteriological studies on methicillin -resistance Staphylococcus Aureus (MRSA) isolated from cow's mastitis milk
Alaa
Anter Saad
An enormous problem reported worldwide namely bovine mastitis caused by MDR( multi-drug resistant) S.aureus results in prolonged antibiotic treatment and livestock death. Mastitis caused by pathogenic S.aureus strains led to a focused study on surveillance of antibiotic susceptibility along with phenotypic and genotypic characterization. The isolation of Staphylococcus aureus to the determination of antibiotic susceptibility pattern from obtained cow’s milk samples was the aim of the current study. Cows affected by mastitis from different farms in Kalyuobia Governorate, one hundred milk samples were conducted for S.aureus isolation with a prevalence of 30% where 20% isolates were coagulase positive (CoPS) and 10% isolates were coagulase negative CoNS (Gram staining, oxidase, catalase, DNase, haemolysis, and the coagulase test were employed for bacterial identification). The majority of the strains (COPS) n=20 were profiled revealing antimicrobial sensitivity to be multidrug resistant. The occurrence of oxacillin susceptible mecA strains positive strains ( OS-MRSA) was revealed in 6 strains for the first time. Identification of MRSA strain by detection of mecA gene in the PCR .
PCR
MecA gene
Methicillin -resistant staphylococcous aureus (MRSA)
2021
07
01
154
158
https://bvmj.journals.ekb.eg/article_193627_7a825d64411b8506bd4a353edbd64185.pdf
Benha Veterinary Medical Journal
1110-6581
1110-6581
2021
40
2
Isolation of Salmonella species in freshly dead rabbit samples in Qalubiya Governorate, Egypt
lamiaa
elmenyawy
This study was carried to examine one hundred and sixty-three different freshly dead rabbits’ corpses collected from different husbandries of rabbit rearing located in Qalubiya governorate, suffered from mortalities along the period from January, 2020 to March 2021. From each collected corpse sample, five internal organs represented by liver, kidney, spleen, lung and intestine were collected for bacteriological investigation for the presence of Salmonella species; after which serological identification of Salmonella isolates was performed then sensitivity test was applied to detect resistance to some antibiotics groups Resistance genes (AadB ,qnrS and TetA(A))are detected . Results of bacteriological examinations revealed that isolation of thirty-one different Salmonella species, where isolated from liver 7, kidney 5, spleen 10, lung 3, and intestine 6 isolates with incidence of 22.5, 16.1, 32.3, 9.6, and 19.35% respectively. Moreover, serological identification clarified that out of thirty-one isolates, 9(29.03%) strains were S. Arizonae, 5(16.12%) strains were S. Kentucky, 7(22.58%) strains were S. Enteritidis, and 8(25.80%) strains were S. Typhimurium, and 2(6.45%) strain was S. Banana. Referring to the obtained results, Salmonella was regarded as significant bacteria responsible for several morbidity and mortalities cases in rabbit farms. Antimicrobial susceptibility was applied on Salmonella strains and referred that salmonella have high resistance to streptomycin, tetracycline and quinolones group. Moreover, resistance genes (aadB, qnrs, and tetA(A) were also detecting using PCR.
Salmonella species
bacteriological detection
serological identification
antimicrobial susceptibility and detection of resistance gene by using PCR
2021
07
01
159
163
https://bvmj.journals.ekb.eg/article_193624_77d54662bd517997dd6972ac0df42a68.pdf
Benha Veterinary Medical Journal
1110-6581
1110-6581
2021
40
2
Adaptation and Comparative Growth Behaviour of Egyptian SPV isolates in Vero
Gabr
Elbagory
Emad
Aboul Soud
Nesma
Hassan
Abstract: Although primary lamb testis (LT) culture seemed to be sensitive and the most suitable for adaptation of recently isolated sheep pox viruses, giving rapid and complete Cyto Pathic Effect (CPE), these cells were difficult to be maintained as a monolayer cell line, also these primary and secondary cultures still have many of the detrimental, inherent characteristics and contaminating elements. Therefore, the use of more stable cell lines (Vero cells) become necessary for economic SPV seed vaccine preparation. Therefore, three SPV isolates, which previously passaged and adapted in lamb testis culture, (isolates with titre reaching to log 10 6.5 TCID 50/ ml), were chosen to be transferred and propagated on cell lines as Vero. With further studies on its growth curve and growth kinitics to identify the growth behavior of these isolates, followed by detection of the virus antigen in the infected Vero cells by Indirect Florescent Antibody Technique (IFAT) as the first step for antigenic identification of the isolated adapted virus. Key words: lamb testis, cytopathic effect, sheep pox virus, growth kinetics.
lamb testis
cytopathic effect
sheep pox virus
growth kinetics
2021
07
01
164
167
https://bvmj.journals.ekb.eg/article_193635_ff10aaedb974f6dfd1f98c1491c00503.pdf
Benha Veterinary Medical Journal
1110-6581
1110-6581
2021
40
2
Efficacy of live attenuated and inactivated bivalent vaccine against canine distemper and canine parvo viruses in dogs
Gabr
Elbagory
shaimaa
elkhatib
Mohammad
Khodier
Through the present work we succeeded to prepare live attenuated and inactivated canine distemper (CD) using Snyder Hill strain and canine parvo (CP) vaccines using CPV type 2a srain either in mono or bivalent formulae aiming to establish the most safe and potent vaccine formula that enable puppies to withstood both virus infections. It was found that monovalent and bivalent attenuated vaccines induced higher and longer duration of immunity, showed protective CD antibody titers (64-128 by SNT and 2.4log10 by ELISA) up to 12 months post vaccination while inactivated ones induced lower and shorter protective immune levels (32 by SNT and l.8 log10 by ELISA) for 10 months post vaccination. Also, CP antibody levels remained with high levels (64-128 by SNT and 2.5 by ELISA) up to 12 months as induced by attenuated mono and bivalent vaccine and with lower protective values (32-64 by SNT and 1.5- 1.9log10 by ELISA) by inactivated vaccines up to 11 months. Anyhow, it could be said that the bivalent CD and CP attenuated or inactivated vaccines can provide vaccinated puppies with specific protective antibodies against the two viruses.
Canine distemper virus vaccine
Canine Parvovirus vaccine, SNT, ELIZA, puppies
2021
07
01
168
173
https://bvmj.journals.ekb.eg/article_193630_3b474e43807fc2e9619c613243801b81.pdf
Benha Veterinary Medical Journal
1110-6581
1110-6581
2021
40
2
Isolation, identification and characterization of Capripox virus among clinically infected cases of small ruminants in different Egyptian governorates
Gabr
Elbagory
Emad
Aboul Soud
Nesma
Hassan
Sheep pox virus (SPV) is a member of the genus Capripoxvirus, of family Poxviridae, which affect sheep and goats causes significant economic losses. The present study was applied for isolation and identification of Sheep pox virus from clinically affected sheep during 2017 to 2019 in five Egyptian governorates, El Wadi Al-Gadid, Marsa -Matrouh, Giza, Kafr El-Sheikh and Menufya. Despite the obligatory use of sheep pox vaccine for vaccination of sheep and goats in Egypt, but some factors hinder the mass vaccination for all sheep and goats’ population like the grazing nutritional behaviour, poor management, scare feeding and inadequate veterinary services. The virus was isolated from skin lesions and lymph nodes on chorio allantoic membrane of Embryonated Chicken Eggs (ECE), with typical pock lesions, followed by identification on lamb testis culture (LT) showing the characteristic cytopathic effect. Similarly, the convential PCR depending on P32 gene, and by multiplex PCR give the same results proving that the isolated virus is SPV.
pox
sheep
Egypt
skin
2021
07
01
174
178
https://bvmj.journals.ekb.eg/article_193657_c8c21d86ba7a5d0da9e560cc3373f40b.pdf
Benha Veterinary Medical Journal
1110-6581
1110-6581
2021
40
2
The efficacy of both locally prepared vaccines against foot and mouth disease in cattle in Monofeia governorate
Ehab F.
Shaban
Faysal K.
Ibrahim
Rania
Abo-Sakaya
Sayed A. H.
Salem
Vaccination was the main approach to control spreading of FMD disease in Egypt. This study was carried out for evaluation of vaccination by governmental and prevailing local vaccines in cattle sera in menofia governorate during the period from October 2018 to May 2019. A total 130 serum samples were collected to evaluate the antibodies against FMDV. 30 samples were used as control, 50 samples collected from animals received local governmental vaccine and 50 samples from animals received local prevailing vaccine. 49 out of 100 serum samples (37.7%) were positive and 81 out of 130 serum samples (62.3%) were negative for NSP. Results were (0.0), 3(3.7%) pre-vaccination and 27(33.33%), 18(22.22%) 1st MPV- 25(30.86%), 18(22.22%) 2nd MPV-15(18.52%), 13(16.05%) 3rd MPV and (0.0), 9(11.11%) 4th MPV for serotype A. For serotype (O): 9(11.1%), 10(12.4%) pre-vaccination- 36(44.44%), 28(34.56%) 1st MPV-33(40.74%), 27 (33.33%) 2nd MPV-24(29.63%), 20(24.7%) 3rd MPV-14(17.3%), 12(14.80%) 4th MPV. For serotype (SAT2): 4(4.9%), 24(4.93%) pre-vaccination-30 (37.03%), 21(25.93%) 1st MPV-27(33.33%), 21(25.93%) 2nd MPV- 18 (22.22%), 14(17.3%) 3rd MPV-3 (3.71%), 4(4.93%) 4th MPV. In Conclusion. FMD controlling requires vaccines that will supply disparity of infected from vaccinated animals.
cattle
FMDV
NSP
ELISA kit
MPV
2021
07
01
179
183
https://bvmj.journals.ekb.eg/article_193633_59c95842e79b258cb2c348f2a88ddac1.pdf
Benha Veterinary Medical Journal
1110-6581
1110-6581
2021
40
2
Estimation of Non-genetic Parameters Affecting Total Milk Yield and Occurrence of Mastitis in Holstein Friesian Dairy Cows
Zienab
Abo-Gamil
Hadeel
El-Qaliouby
Eman
Manaa
Sherif
Ramadan
This study was performed on 1480 productive records collected from private farm of Holstein Friesian cows. In addition, collection of 70 milk samples for the measurement of somatic cell count (SCC). This study aims to evaluate the effect of some non-genetic factors (days in milk (DIM), peak milk yield (PMY), dry period (DP), calving interval (CI), parity, and season of calving) on total milk yield (TMY). And studying the effect of 305-day milk yield (305-DMY), peak milk yield (PMY), days in milk (DIM), parity and season of calving on mastitis in Holstein Friesian cows. All data were analyzed by general linear model (GLM) using SAS software ver.9.1.3. The current results revealed that DIM and parity had a significant effect on TMY, where the highest milk yield was obtained when DIM were more than 357 days and in the 3rd lactation season. Season of calving showed a significant effect on mastitis disease, where the highest incidence of mastitis was recorded for spring calvers.
Holstein Friesian
productive traits
reproductive traits
total Milk yield
and Mastitis disease
2021
07
01
184
188
https://bvmj.journals.ekb.eg/article_193681_b84534b3455ce9c5d5ed461c30b89e02.pdf
Benha Veterinary Medical Journal
1110-6581
1110-6581
2021
40
2
Effect of Some Non-Genetic Factors on 305-day Milk Yield and Lactation Persistency in Holstein Friesian Cows
Zienab
Abo-Gamil
Hadeel
El-Qaliouby
Eman
Manaa
Sherif
Ramadan
This study was performed on productive data collected from private farm of Holstein Friesian cows. The objective of this study was to evaluate the effect of days in milk (DIM), peak milk yield (PMY), dry period (DP), calving interval (CI), parity, and season of calving on 305-day milk yield (305-DMY). And the effect of 305- day milk yield (305-DMY), peak milk yield (PMY), days in milk (DIM), parity, and season of calving on lactation persistency. We found that DIM, parity, and PMY had significant effect on 305-DMY, the highest milk production was obtained when DIM more than 357 days and in the 1st lactation. Moreover, PMY, 305-DMY, and season of calving had significant effect on lactation persistency. In addition, average estimated breeding value (EBV) for 305-DMY, PMY and CI was higher in sire than in cow and dam. Also, Average estimated breeding values for lactation persistency, DP, and DIM were higher in dam than in cow and sire.
Holstein Friesian cows
productive traits
reproductive traits
305-day milk yield
Lactation Persistency and breeding values
2021
07
01
189
194
https://bvmj.journals.ekb.eg/article_193682_67f4a790c2be69355850f9352c6c4f3e.pdf
Benha Veterinary Medical Journal
1110-6581
1110-6581
2021
40
2
Effect of Diet Containing Different Levels of Dried Azolla meal on Some Economic Evaluation Parameters of Broiler Chickens
Eman
Hamed
Eman
Ramdan
sanad
Atallah
This study was conducted to investigate the effect of using different levels of dried Azolla (DA) on some economic evaluation parameters of broiler chickens. A total of 200 healthy unsexed one-day-old broiler chicks (Cobb type) were allocated randomly in to four groups (50 chicks/each group). Each group consists of three replicates. DA was chemically analyzed and used as 0%, 4%, 8% and 12% to formulate 4 balanced experimental diets (Control, T4, T8, and T12, respectively). Results revealed high positive and negative correlation among the different studied productive and economic variables. Total production function among the experimental groups, showed a significant positive effect of the changes in total feed intake (TFI), and feed cost on body weight (BW). Broiler group fed 4% DA revealed a significant positive effect of the changes in TFI, and feed cost on BW. And a significant effect of changes in total cost (TC) on total return (TR).
Cobb chickens
Dried Azolla
and Economic evaluation
2021
07
01
195
201
https://bvmj.journals.ekb.eg/article_193686_b40621261cbcae2abb61f5e65462ebc9.pdf
Benha Veterinary Medical Journal
1110-6581
1110-6581
2021
40
2
Impact of dietary supplementation of vitamin E (alpha-tocopherol acetate) on genetic expression of inflammatory cytokines and growth efficiency of broiler chickens
aya
Elshawash
Fatma
Elgendy
Olla
Khalifa
Shabaan
Hemeda
This research was undertaken in order to determine the impact of nutritional vitamin E on genetic expression of Interferon-gamma (IFN-γ) and Interleukin-1β (IL-1β) as pro-inflammatory and Interleukin-4 (IL-4) and Interleukin-10 (IL-10) as anti-inflammatory cytokines in spleen and liver, heamagglutination inhibition antibody titers (HI titers) against Newcastle Disease Virus (NDV), total protein, albumin, globulin and growth efficiency of broiler chickens. Seventy two one-day-old Cobb broiler chicks had been allocated randomly into two groups. Each group has 3 replicates, each with 12 chicks. The first group was given basal diet. The second group was fed the basal diet with vitamin E (100 mg/kg diet). The obtained results revealed that dietary supplementation of vitamin E significantly (p < 0.05) increased growth efficiency, total protein, albumin, globulin and the antibody titers to NDV compared to the control group. Dietary supplementation of vitamin E significantly (P<0.05) decreased the expression of pro-inflammatory (IL-1β, IFN-γ) and anti-inflammatory (IL-4 and IL-10) cytokines in spleen and liver. In Conclusions: vitamin E supplementation (100 mg/kg diet) can enhance growth efficiency, serum total protein, albumin, globulin and humoral immunity, down-regulate pro-inflammatory and anti-inflammatory cytokines gene expression in broiler chickens.
Broilers
Vitamin E
Cytokines
Humoral Immunity
Growth efficiency
2021
07
01
202
206
https://bvmj.journals.ekb.eg/article_193659_d9e1f643cbf303cf67e50546e6bc4a05.pdf
Benha Veterinary Medical Journal
1110-6581
1110-6581
2021
40
2
Prevalence of some pathogenic bacteria in dairy products
Hemmat
Eltokhy
Hamdi
Abdelsamei
Hend
El barbary
marionette
Nassif
Ours study was planned to bacteriologically and molecularly record the prevalence of Escherichia coli (E. coli), Bacillus cereus (B. cereus), and Staphylococcus aureus (S. aureus) in a total of 150 milk and milk products (raw milk, soft cheese (Kareish and packed feta cheese "15 of each"), yoghurt and raw cream samples (30 of each) which were be taken from different supermarkets, dairy shops and street vendors at Qalubiya Governorate, Egypt. The samples were subjected to bacteriological examinations and identification for isolation of E. coli, B. cereus and S. aureus. Results illustrated that the incidence of E. coli, B. cereus and S. aureus in the total of the examined samples were 47.5, 3.3 and 70.83%, respectively. In addition, the examined iss and fimH genes of E. coli, icaD and hlg genes of S. aureus, nhe and cytK genes of B. cereus were detected in all of the examined isolates indicating pathogenic virulence of the isolated strains. In conclusion, raw milk and traditional dairy products (cream, kareish cheese and yoghurt) which exposed to sale in markets revealed low hygienic quality that may be referred to neglected sanitary measures adopted during the production and storage, so it is important to improve the quality of milk products to safe guard the consumers health against the pathogenic microorganisms hazards.
Pathogenic bacteria
milk and dairy products
Bacteriological Quality
PCR
Qalubiya Governorate
2021
07
01
51
55
https://bvmj.journals.ekb.eg/article_221784_b21cf6e1104b549a4546d1bd736490f9.pdf