Benha University; Faculty of Veterinary MedicineBenha Veterinary Medical Journal1110-658142120220401Incidence of pseudomonas specises and effect of their virulence factors on milk and milk products.1524034310.21608/bvmj.2022.103086.1481ENRehabAtiaFood Control Department, Faculty of Veterinary Medicine, Benha University, Banha, EgyptHamdyMohamedFood Control Department, Faculty of Veterinary Medicine, Benha University, Banha, EgyptNahlaAbo ElRoosAnimal Health Research Institute, Shebin El Koom Branch, EgyptDinaAwadFood Control Department, Faculty of Veterinary Medicine, Benha University, Banha, EgyptJournal Article20211029Pseudomonas species are mainly associated with spoilage of dairy products due to their proteolytic activity . Therefore, the goal of the present study was to isolate pseudomonas spp and then identify their virulence factors that have a bad impact on milk qulity . One hundred milk and milk products samples from ( raw milk ,Karish cheese , youghrt and Ice cream) divided to 25 samples from each one were collected and examined to found that Pseudomonas and then identify their species using PCR technique ps. aeuroginosa was present in 20 ,16 ,8 and 8 %in the examined raw milk , karish cheese ,youghrt and ice cream respectively while ,ps. Fluorescence present in 28, 20,12 and 8% respectively in examined samples in the meanwhile ps. Putida was present in 8 and 4% that not indicated youghrt and ice cream , ps.diminuta and ps. Fragi 12 and 8 % respectively in examined samples of raw milk and karish cheese while not indicated in youghrt and ice cream giving incidence percent of pseudomonus spp in examined samples of raw milk , karish cheese , youghrt and ice cream 56 % , 24% and 20% respectively.<br /> Pseudomonas aeuroginosa isolates were subjected to PCR technique to detect the alkaline protease gene oprl, opsl and txoA its virulence genesThe results reveal proteolytic activity and presence of these virulent genes in milk and their spoilage potential at different temperatures..https://bvmj.journals.ekb.eg/article_240343_1a3f6efc38511ab435037c7362e7dfb4.pdfBenha University; Faculty of Veterinary MedicineBenha Veterinary Medical Journal1110-658142120220401Assessment of Antibacterial Impact of Onion Powder on Escherichia coli contaminating broiler chicken cuts61126843610.21608/bvmj.2022.95162.1469ENKamla AdamMohammedPreventive Medicine and Public Health Department, Faculty of Veterinary Medicine, Omar Al- Mukhtar UniversityRanda MahmoudHamadPreventive Medicine and Public Health Department, Faculty of Veterinary Medicine, Omar Al- Mukhtar UniversityAbdulsalamSalehPreventive Medicine and Public Health Department, Faculty of Veterinary Medicine, Omar Al- Mukhtar UniversityHana EalomaAkwietenPreventive Medicine and Public Health Department, Faculty of Veterinary Medicine, Omar Al- Mukhtar UniversityMuhanid MohammedAbd AlazizPreventive Medicine and Public Health Department, Faculty of Veterinary Medicine, Omar Al- Mukhtar UniversityJournal Article20210912The present study aimed to evaluate the efficacy of onion powder on Enteropathogenic E. coli strains recovered from broiler chicken meat. A total of 50 random samples of freshly slaughtered broiler chicken cuts which were collected from poultry retail shops in a trial for isolation and identification of Enteropathogenic E. coli. In addition, an experiment was designed to evaluate the effectiveness of addition of onion powder as natural preservative to control the contamination of broiler chicken cuts with Enteropathogenic E. coli. It was found that the total incidence of E. coli in broiler chicken cuts was 54% and the incidence was higher in the examined samples of thigh (60 %) than the samples of breast (48 %) with significant difference between the incidences. In addition, serological identification showed that the incidence of E. coli O867 (42.30 %) was lower than that of E. coli O125 (57.70 %) by adding of onion powder by different concentrations on artificially inoculated chicken meat cuts with E. coli O125, it was observed that addition of onion powder by concentration of 3% scored the highest Reduction % in E. coli counting compared to that of 2 and 2.5% concentrations especially at 72 hours after treatment.https://bvmj.journals.ekb.eg/article_268436_778199f85dbbefaaa0434fb688e349b3.pdfBenha University; Faculty of Veterinary MedicineBenha Veterinary Medical Journal1110-658142120220401Mycological quality of some chicken meat cuts in Gharbiya governorate with special reference to Aspergillus flavus virulent factors121624034710.21608/bvmj.2021.100118.1476ENAymanGhanemFood Security Sector, Egyptian Armed Forces, EgyptFahimShaltoutProfessor of Meat Hygiene,
Department of Food Control,
Faculty of Veterinary Medicine, Moshtohor,
Benha University, Egypt.
Member of the Egyptian Organization for Standerization and Quality : Meat Hygiene Committee.
Memberhttps://orcid.org/myGamal IbrahimHeikalFood Hygiene Dept., Animal Health Research Institute (Tanta branch), ARC, EgyptJournal Article20211008Contamination with mold and yeast, and their toxins is considered one of the most dangerous hidden pollutants that threaten the health of the consumer. The presence of mycotoxins in various foods has been recorded, despite their apparent safety for human consumption. So, an investigation was conducted to evaluate the mycological quality, with special reference to the presence of toxigenic Aspergillus species, by culture method and aflatoxin producing genes molecularly in a total of 75 random samples of chicken cuts represented by wing, breast and thigh (25 of each) that were collected from various groceries and poultry shops located at Gharbiya governorate, Egypt. Results revealed that breast samples had the highest mycological counts (2.85 CFU/g). Consequently, isolated strains were subjected for mycological classification; where results revealed detection of Aspergillus, Penicillium, Cladosporium, Rhizopus and Alternaria species in various ratios in the examined samples. Moreover, Aspergillus sp. in 36%, 48% and 40% of the examined wing, breast and thigh samples, respectively. Furthermore, mycological identification of the isolated Aspergillus strains showed the presence of A. niger, A. flavus, A. fumigatus, A. terreus and A. parasiticus in 16%, 13.3%, 10.6%, 1.3% and 1.3% of the total population of the examined samples.https://bvmj.journals.ekb.eg/article_240347_969e78417908c66203f23fb9533433d3.pdfBenha University; Faculty of Veterinary MedicineBenha Veterinary Medical Journal1110-658142120220401Ameliorative effect of coenzyme Q10 against deltamethrin-induced renal toxicity in broiler chickens172424035510.21608/bvmj.2022.117110.1499ENMohamedAboubakrDepartment of pharmacology, Faculty of Veterinary Medicine, Benha University, 13736 Moshtohor, Toukh, Qaliobiya, EgyptAliAllamDepartment of Pharmacology, Faculty of Veterinary Medicine, Benha University, 13736 Moshtohor, Toukh, Qaliobiya, Egypt.AlshaimaaSaidBiochemistry Department, Faculty of Veterinary Medicine, Benha University, 13736 Moshtohor, Toukh, Qaliobiya, Egypt0000-0001-5655-9514SamarIbrahimforensic medicine and toxicology, faculaty of veterinary medicine , benha university0000-0001-5530-9521GehanYoussefDepartment of Forensic Medicine and Toxicology, Faculty of Veterinary Medicine, Benha University, 13736 Moshtohor, Toukh, Qaliobiya, Egypt.Journal Article20220118The ameliorating effect of Coenzyme Q10 (CoQ10) supplementation was evaluated on broiler chickens intoxicated with deltamethrin (DM) through serum biochemical analysis, antioxidant capacity, and pathological examination. Cobb broiler chicks (60) aged 1 day were allocated into 4 experimental equal groups. For each group, three replicates of 5 chicks were used. The first group (control) received only the basal diet, the second group received CoQ10 (40 mg/kg diet), the third group received DM (300 mg/kg diet), and the fourth group received both DM (300 mg/kg diet) and CoQ10 (40 mg/kg diet). The experimental period was 35 days has been given to the last 3 groups. DM intoxication was associated with significant increases in creatinine, urea, malondialdehyde (MDA), and a drop in levels of reduced glutathione (GSH) and superoxide dismutase (SOD). In addition, DM increased blood cholesterol, triacylglycerols, and low-density lipoprotein (LDL), while lowered high-density lipoprotein (HDL). Caspase-3 and B cell lymphoma 2 (BCl2) were substantially unregulated by DM in the kidney tissues. The microscopic examination of the kidneys revealed congestion of the renal blood vessels with necrosis of the lining epithelium of the renal tubules. Concurrent supplementation of CoQ10 with DM resulted in a notable improvement in estimated parameters compared to the DM group. Dietary CoQ10 is therefore advised because of its preventive properties against DM-induced renal toxicity in broilers.https://bvmj.journals.ekb.eg/article_240355_ec46ad89fc27b1e9eafb0533d2f3c57d.pdfBenha University; Faculty of Veterinary MedicineBenha Veterinary Medical Journal1110-658142120220401Ameliorative impact of coenzyme Q10 supplementation on deltamethrin-induced hepatic damage in broiler chickens253224036910.21608/bvmj.2022.117104.1498ENMohamedAboubakrDepartment of pharmacology, Faculty of Veterinary Medicine, Benha University, 13736 Moshtohor, Toukh, Qaliobiya, EgyptAliAllamDepartment of pharmacology, Faculty of Veterinary Medicine, Benha University, 13736 Moshtohor, Toukh, Qaliobiya, Egypt.AlshaimaaSaidBiochemistry Department, Faculty of Veterinary Medicine, Benha University, 13736 Moshtohor, Toukh, Qaliobiya, Egypt.0000-0001-5655-9514SamarIbrahimforensic medicine and toxicology, faculaty of veterinary medicine , benha university0000-0001-5530-9521GehanYoussefDepartment of Forensic Medicine and Toxicology, Faculty of Veterinary Medicine, Benha University, 13736 Moshtohor, Toukh, Qaliobiya, Egypt.Journal Article20220118Deltamethrin (DM) is a synthetic insecticide that is commonly used in agriculture and veterinary medicine. Furthermore, animal and human studies have shown that DM has a negative impact on the liver. This study was carried out to evaluate the protective effect of Coenzyme Q10 (CoQ10) supplementation against DM hepatotoxicity in broiler chickens. Sixty chicks were divided into 4 groups. First group (basal diet), second group (40 mg CoQ10 /kg diet), third group (300 mg DM/kg diet), and the fourth group (300 mg DM /kg diet) and (40 CoQ10mg/kg diet). The treatment was administered to the final three groups for 35 days. DM declined body weight (BW), body weight gain (BWG), and increased feed conversion rate (FCR). Significant increases in aspartate aminotransferase (AST), alanine aminotransferase (ALT), malondialdehyde (MDA), with a significant drop in levels of reduced glutathione (GSH) and superoxide dismutase (SOD) were also recorded in this group. Caspase-3 and B cell lymphoma 2 (BCl2) were substantially upregulated by DM in liver tissues. In addition, alterations in histopathology as severe congestion and mononuclear inflammatory cellular infiltration of the hepatic parenchyma with vacuolation of the hepatic cells were recorded in the examined liver of chickens intoxicated with DM. Concurrent CoQ10 supplementation with DM resulted in a significant improvement in estimated parameters when compared to the DM group. Because of its protective effects against DM-induced hepatotoxicity in broilers, dietary CoQ10 is recommended.https://bvmj.journals.ekb.eg/article_240369_cec8ab8d5471527d55944f60c3b5f233.pdfBenha University; Faculty of Veterinary MedicineBenha Veterinary Medical Journal1110-658142120220401Preparation and evaluation of metformin-loaded chitosan nanoparticles for biomedical applications333824037710.21608/bvmj.2021.88233.1455ENAya Allah MostafaSalemDepartment of pharmaceutical biotechnology ,faculty of biotechnology ,must universityJournal Article20210809Nano drug delivery has been recently used to improve the stability and bioavailability of drugs. The current work deal with the preparation and characterization of metformin loaded in chitosan nanoparticles (M-CHNPs) for medical purposes. Chitosan nanoparticles (CHNPs) were prepared by ionotropic gelation of chitosan with tripolyphosphate anions. Free chitosan nanoparticles and M-CHNPs were characterized via X-ray diffraction (XRD), dynamic light scattering (DLS), Raman spectrometer, high-resolution transmission electron microscopy (HRTEM), zeta sizing, BET surface area, and atomic force microscope (AFM). The drug encapsulation efficiency, drug loading capacity, and in vitro release rate were also investigated. ). The results showed that M-CHNPs were successfully prepared in pure form without any undesirable products as showed in X-ray diifraction pattern and Raman spectra. Also, the obtained M-CHNPs had an average hydrodynamic size of 78 nm, zeta potential of -28 mv, and showed a drug encapsulation efficiency and drug loading capacity up to 91%. In vitro release study indicated that metformin release from M-CHNPs was pH-dependent, where it released with rates 51%, 40%, and 10% pH 1.2, 6.8, and 7.4 respectively.https://bvmj.journals.ekb.eg/article_240377_7e1fd65dfeefeda8fab568b3f772b3f8.pdfBenha University; Faculty of Veterinary MedicineBenha Veterinary Medical Journal1110-658142120220401Biochemical and histopathological evaluation of the effect of metformin and metformin nanoparticles against alloxan-induced diabetes in Rats394524884210.21608/bvmj.2022.128967.1508ENAya Allah MostafaSalemDepartment of pharmaceutical biotechnology ,faculty of biotechnology ,must universityJournal Article20220322Nanoparticles drug delivery systems are nanometric carriers that enhance drugs delivery and therapeutic action. The current study aimed to investigate the effect of daily oral administration of metformin and metformin nanoparticles (45 mg/kg b. w.) for 28 days on rats subjected to type 2 diabetes.24 male albino rats were divided into 4 groups, group 1 normal control, group 2 diabetic control, group 3 diabetic rats treated with metformin drug, group 4 diabetic rats treated with metformin chitosan nanoparticles drug with dose (45 mg/kg b. w.) for 28 days. Compared to diabetic control, a significant increase in body weight and decreased organs weights (liver, heart, kidneys) were recorded in diabetic rats treated. With metformin. Also, data was recorded in a significant decrease in serum glucose levels of treated diabetic rats compared to diabetic control rats. Increased serum levels of liver function (ALT, AST, ALP) and kidney function (Urea, Creatinine, Uric acid) parameters were detected in diabetic rats. Treatment with metformin and metformin nanoparticles caused a significant decrease in AST and ALT activity along with a significant decrease in creatinine and urea levels, the effects that were reversed upon metformin administration with the nanoparticles. However, the metformin nanoparticles have shown a superior effect. Various histopathological alterations were detected in the pancreas, liver, and kidney of diabetic animals. Alleviation of the histopathological changes was achieved following metformin with the nano-metformin affording better protection. In conclusion, The effect of metformin nanoparticles appears better than metformin in alloxan-induced diabetes in rats.https://bvmj.journals.ekb.eg/article_248842_a8f99e426f0384062fdb68ab7a96c03d.pdfBenha University; Faculty of Veterinary MedicineBenha Veterinary Medical Journal1110-658142120220401Effect of Layer Breeds on the Productivity and Profitability of Layer Farms under Egyptian Condition465024043310.21608/bvmj.2022.117301.1500ENAmira MohamedAbd-El HamedAnimal Wealth Development Department, Faculty of Veterinary Medicine Benha University0000-0002-2559-4314ZienabHamdyAnimal wealth development-veterinary medicin-benha universityJournal Article20220119This study was carried out during the period extended from the year 2019 to the year 2021 on four-layer breeds (Lohmann (LB), ISA Brown, Hy-Line W-36, and Hy-Line Brown) of the layer farms in EL-Menofia governorate, the data were collected from the accurate health and production records and also, by using the structured questionnaires methods. This study aims to determine and highlight the effect of different layer breeds on the productivity and profitability of layer farms under Egyptian condition, also it indicated that the layer breed is the major factor that can affect the egg production and net profit of layer farms, This study concluded that the egg production significantly differed among the layer breeds it was the highest for ISA Brown breed (35603.97 eggs/100 hens/cycle), while it was the lowest for Hy-Line Brown breed (25368.37 eggs/100 hens /cycle), also the total return and net profit differed significantly among the layer breeds and they were the highest for ISA brown breed (49604.26 and 6426.18 EGP/100 hens, respectively), while they were the lowest for Hy-Line Brown breed (35859.55 and 497.79 EGP/100 hens, respectively). Finally, we concluded that the ISA Brown breed is the most profitable breed to be reared in the layer farms.https://bvmj.journals.ekb.eg/article_240433_ea50d1b4043ccf9e06483f95c873f252.pdfBenha University; Faculty of Veterinary MedicineBenha Veterinary Medical Journal1110-658142120220401Ameliorative Effect of Spirulina Platensis against Cadmium Toxicity in Broiler Chickens515524043010.21608/bvmj.2022.111546.1490ENSaraBerbeshFronsic medicine and toxicology, faculty of veterinary medicine, Benha university, Benha, Egypt.RagabEl-ShawarbyForensic Medicine and Toxicology Department, Faculty of Veterinary Medicine, Benha UniversityElhamEl-ShewyForensic Medicine and Toxicology Department, Faculty of Veterinary Medicine, Benha UniversitySaharEl-SheshtawyBiochemistry, Toxicology and Nutritional Deficiency Department, Animal Health Research Institute, Tanta Branch (AHRI), Agriculture Research Centre (ARC)SaidElshafaeDept. of Pathology, Faculty of Veterinary Medicine, Benha University, Tukh, QalyobiyaJournal Article20211218Cadmium (Cd) is an environmental heavy metal non-biodegradable pollutant, and its ubiquity ensures its recurrent exposure to human and animals. Cd chloride poses an emerging threat to the poultry industry as well as humans who consume Cd-intoxicated chickens due to its bioaccumulative properties. Spirulina platensis is a cyano-bacterium with strong antioxidant, anti-inflammatory, hepato-protective, nephroprotective as well as growth, productive and reproductive enhancer of animal and poultry. The main aim of this study was to investigate the potential protective effect of Spirulina platensis against Cd-induced oxidative damage in broiler chickens. Sixty broiler chicks were divided randomly into four equal groups (15 each). The chicks received either water (group I, control) or Spirulina (group II) or Cd chloride (group III) or both Cd chloride and Spirulina (group IV). Our results showed that Cd intoxicated chickens co-treated with Spirulina platensis had significant improvement in growth parameters i.e., final body weight, daily weight gain and feed conversion rate. In addition, a significant decrease in liver enzymes, renal parameters, total Cd residues in tissues and oxidative parameters was seen in group IV compared to Cd chloride intoxicated chickens. The level of superoxide dismutase and total antioxidant capacity were higher in-group IV. Histopathologically, Spirulina attenuated the hepatorenal toxicity of Cd chloride in group IV evidenced by less degeneration changes in liver and kidneys compared to group III. In conclusions, Spirulina protected broiler chickens against Cd induced liver and kidney damage. Spirulina could be a beneficial supplement in chicken ration to counteract potential Cd chloride toxicity.https://bvmj.journals.ekb.eg/article_240430_b7e8c44d820c75c971fad935e0f79e3c.pdfBenha University; Faculty of Veterinary MedicineBenha Veterinary Medical Journal1110-658142120220401Characteristics of Fluoroquinolone-Resistant Escherichia Coli from Broiler Chickens with Colibacillosis566024042610.21608/bvmj.2021.102145.1480ENMohamed Saber SobhyHusseinAnimal Health Research InstituteHanan AliFahmyAnimal Health Research Institute, ARC, Egypt.Ashraf AwadAbd El TawabDepartment of Bacteriology, Immunology and Mycology, Faculty of Veterinary Medicine, Benha UniversityJournal Article20211021Avian pathogenic Escherichia coli causes high mortalities and substantial economic losses in the poultry industry worldwide, a problem that intensified with the increasing antibiotic resistance. In this study, we characterized fluoroquinolones resistance in E. coli from broiler chickens with colibacillosis. A total of 150 visceral organs collected from broiler chickens with postmortem colibacillosis lesions were subjected to bacteriological and biochemical examination. Out of them, 89 samples (59.3%) were found positive for E. coli. The most predominant serogroups were O125 (23.6%), O44 (16.6%), O127 (15.7%), and O18 (11.2%). The antimicrobial susceptibility testing of the recovered E. coli isolates against seven quinolone antibiotics revealed that 88.8% of the isolates were resistant to at least one tested antibiotic, while 25.8% were resistant to all of them. The highest resistance rates were observed against flumequine (77.5%) and nalidixic acid (73%), while the lowest resistance rate was observed against levofloxacin (29.2%). A total of 12 isolates displaying phenotypic resistance to fluoroquinolones were then screened for plasmid-mediated quinolone resistance (PMQR) genes qnrA, qnrB, qnrS and aac(6′)-Ib-cr by PCR. The results showed that 66.7% of the isolates were positive for qnrS gene, while aac(6′)-Ib-cr, qnrA and qnrB genes were not detected in any isolate. The high occurrence of fluoroquinolones resistance and fluoroquinolones resistance genes in avian pathogenic E. coli is alarming, and urgently requires effective monitoring of the use of fluoroquinolones in the broiler sector, with regular screening for these resistance genes to evaluate the scale of their threat to veterinary practice and public health.https://bvmj.journals.ekb.eg/article_240426_07181ab25d2d31700af7a1e2ad7f0910.pdfBenha University; Faculty of Veterinary MedicineBenha Veterinary Medical Journal1110-658142120220401Gold Nanoparticle, Physical and Physiological Applications on Cells and Microorganisms.616724038910.21608/bvmj.2022.123233.1503ENSamalAL-JaffMustansiriyah University, College of Science, Biology Department, Baghdad. IraqSuhairAl. SirajMustansiriyah university, Microbiology Department, Baghdad, IraqAlaaSaadMicrobiology Department, Faculty of Veterinary Medicine, Benha University, Egypt.0000-0002-4155-8743Journal Article20220222The manufacture of nanoparticles has resulted in modern developments in nanotechnology. Metallic nanoparticles have been used successfully in biological applications, and gold nanoparticles (AuNPs) are especially noticeable. Gold nanorods and spherical nanorods nanoparticles get a lot of attention. Electronic, surface plasmon resonance, optical, and physicochemical features are some of their inherent qualities. That may be changed by altering the particle's characterizations such as size, shape, or environment, aspect ratio, synthesis ease, and functionalization qualities. Consequence effect on their diversity of applications in biomedicine (cells and pathogenic microorganisms), included the sensing, imaging, targeted, photothermal, drug delivery, and photodynamic therapy. AuNPs are stable and biocompatible, and their capabilities as antibacterial can be improved by altering their shapes and sizes or adding chemicals. AuNPs can also boost the antibacterial properties of laden antibacterial medicines by acting as drug transporters and also could play a stronger antibacterial function for efficient antibacterial methods against several resistant bacteria after being modulated and coupled with other antibacterial medications. Modified AuNPs can be an excellent substance for photothermal therapy to destroy pathogens. Many materials can gain antibacterial characteristics by adding biologically adjusted AuNPs.https://bvmj.journals.ekb.eg/article_240389_f27a6618211fca0a4b90a57ab56aada5.pdfBenha University; Faculty of Veterinary MedicineBenha Veterinary Medical Journal1110-658142120220401Survival and stability of free and nanoencapsulated Lactobacillus casei under simulated gastrointestinal fluid and thermal conditions687224884310.21608/bvmj.2022.126462.1504ENHadeer Abd ElmonaemHassan SalmanMicrobiology department,science faculty,Benha,Alqalubia,EgyptJournal Article20220315The main objective of the current study was to assess the effect of nano-encapsulation on the stability and viability of probiotic bacteria under simulated gastrointestinal digestion and thermal conditions. Purposely, probiotics were encapsulated with (sodium alginate and pullulan) by using mechanical encapsulation technique. Furthermore, developed nano-encapsules were characterized by scanning electron microscope to elucidate the interaction between the capsule matrix and probiotic bacteria. The viability and the stability of probiotic bacteria assessed by subjecting to simulated gastrointestinal, intestinal, thermal and refrigerating conditions. Nano encapsulation exhibited a significant effect on the survival and stability of probiotic bacteria. Overall, the decreasing rate in the viability of probiotic bacteria was observed in all treatments. A rapid reduction in CFU/gram was observed when free probiotic cells were stored at refrigeration temperature 4oC compared to nano encapsulated probiotic bacteria. Not only in vitro gastrointestinal assay, nanoencapsulated bacteria recorded lower reduction in total viable cells than free bacterial cells, but also the results of the viable count in the case of nano encapsulated cells were above the recommended level (106CFU/gram) under thermal conditions as well as in simulated GIT conditions.https://bvmj.journals.ekb.eg/article_248843_0dbcc6da811470dba4a521dfcc1dfcca.pdfBenha University; Faculty of Veterinary MedicineBenha Veterinary Medical Journal1110-658142120220401Diagnosis of caprine pneumonia: impact of vitamin D deficiency and other risk factors in its incidence737924890510.21608/bvmj.2022.115157.1493ENNouraAttiaDepartment of Animal Medicine, Zagazig UniversityYasminBayoumiDepartment of Animal Medicine (Internal Medicine), Faculty of Veterinary Medicine, Zagazig UniversityEslamEisaDepartment of Surgery, Anesthesiology and Radiology, Faculty of Veterinary Medicine, Zagazig UniversityNaderMaherDepartment of Animal Medicine (Infectious diseases), Faculty of Veterinary Medicine, Zagazig University, Zagazig, Sharkia, Egypt.NouraEl SeddawyDepartment of Pathology, Faculty of Veterinary Medicine, Zagazig University, EgyptJournal Article20220108The objectives of this study were to apply different methodsfor diagnosis of caprinepneumonia and to study the correlation between vitamin D concentration and the immunoglobulins (IgG, IgM, IgA) in goats up to one year in response to pneumonia.<br /> From October 2018 to February 2021, a total of 107 baladi goats were includedexamined; 20 apparently healthy goats used as control group (group I, n=20) and 87 pneumonic baladi Baladi goats were diagnosed on the basis of clinical symptoms, ultrasonographic, chest x-ray findings. Serum vitamin D, IgG, IgM and IgA levels were also measured. Pneumonic goats (group II, n= 87) were subdivided into two groups according to their vitamin D levels; pneumonic goat with normal vitamin D level (groupII a, n= 38) and pneumonic goat with decreased vitamin D levels (groupIIb, n= 49). Pneumonic goats were presented with fever, dullness, tachypnoea, bilateral mucoid or mucopurulent/purulent nasal discharge, cough, dyspnea and abnormal lung auscultation. Ultrasonography, the pneumonic consolidation exhibits a liver like echotexture. Abnormality in the chest x-ray revealed increased opacity that may be more gray or white and cotton wool like appearance. Pneumonic goats with reduced vitamin D concentration (group IIb)were also found to havealso revealed significantly lower IgG and IgM concentrations in comparison to both group I and group II a. Vitamin D was positively correlated with IgG. Histopathologically, the pneumonic lesions include interstitial pneumonia, acute suppurative bronchopneumonia and acute fibrinous bronchopneumonia.https://bvmj.journals.ekb.eg/article_248905_9ff06054951d9bd2efc2de1a08005e25.pdfBenha University; Faculty of Veterinary MedicineBenha Veterinary Medical Journal1110-658142120220401Clinical, Hematological, Acute phase proteins and Radiographic changes in different respiratory affections in dogs and cats808524890410.21608/bvmj.2022.129782.1509ENGehad ElsaidElgalfydepartment of internal medicine , faculty of veterinary medicine benha universityYassein MahmoudAbd El-RaofFaculty of veterinary medicine, Benha university ,Department of animal medicine ( internal medicine )Mohamed MohamedyGhanemFaculty of veterinary medicine, Benha university ,Department of animal medicine ( internal medicine )Heba MohamedEl-khaiatFaculty of veterinary medicine, Benha university ,Department of animal medicine ( internal medicine )Journal Article20220326Respiratory affections are important clinical problems recorded in dogs and cats affecting their health condition. This study was carried out on a total of 84 animals including 32 dogs and 52 cats of both sexes and different breeds suffering from different respiratory affections including pneumonia, aspiration pneumonia, Feline upper respiratory diseases(FURD) and Canine infectious respiratory disease(CIRD). Clinical, hematological, biochemical and radiographic changes during these affections were evaluated. In addition the most common incriminated bacteria was isolated and identified. The affected cases showed variable respiratory signs including dyspnea, nasal and ocular discharge, sneezing, cough, abnormal respiratory sound and abnormal lung sound. Hematological changes showed inflammatory leukogram represented by increasing in WBCs and neutrophil count Serum analysis showed marked increase in CRP, SAA and HP levels, with hyperproteinemia and hypoalbuminemia compared to reference value. The most common bacteria isolated from pneumonic cases were Klebsiella(29.1%), E.coli (20.8%), Staph. (16.6%), Pseudomonas (21.5%), Pasteurella (21.5%) Proteus and Serratia(8.3%). Radiographic examinations revealed abnormal radiographic patterns associated with the different affections. The present study concluded that clinical, hematological, biochemical combined with chest radiographic findings are essential for precise diagnosis of different respiratory affections in dogs and cats and the early diagnosis facilitate the prescription of relevant therapy and follow up procedures.https://bvmj.journals.ekb.eg/article_248904_63efa9c8e40fca73e4b1175a3bb8f789.pdfBenha University; Faculty of Veterinary MedicineBenha Veterinary Medical Journal1110-658142120220401Exploring of ovulation time in bitches by utilizing cytological surveyand doppler ultrasonography868924890810.21608/bvmj.2022.104891.1484ENMarwaYousryDiagnostic and Sonography Department, Animal Reproduction Research Institute, Giza, EgyptM. S.FadelDiagnostic and Sonography Department, Animal Reproduction Research Institute, Giza, EgyptG.A.SosaDepartment of Theriogenology, Faculty of Veterinary Medicine, Benha University, EgyptAl Shimaa.El -NabyDepartment of Theriogenology, Faculty of Veterinary Medicine, Benha University, EgyptM.A.AgagDepartment of Theriogenology, Faculty of Veterinary Medicine, Benha University, EgyptJournal Article20211107There are challenges when monitoring peri-ovulatory ovarian activity in domestic bitches which only ovulate once or twice a year. There is difficulty to distinguish between pre-ovulatory follicles and/or corpus luteum. As luteinization of follicles subsists before ovulation, follicles do not collapse after ovulation. Thus, the aim of the present study was to predict the exact day of ovulation. Twenty-seven bitches of mixed breeds were examined daily from onset of proestrus to five days post ovulation by vaginal smear, real-time B-mode and Doppler ultrasonography. Ultrasound was conducted in both standing and lateral recumbency positions using microconvex and linear probes. Vaginal swabs were stained by DIFF QUICK stain. There was high cornification rate (90 %) of vaginal epithelium and substantial increase in follicular size (0.55±0.02 cm) at ovulation day. There was significant increase in ovarian blood perfusion at Day 0 and 1. Maximum coloring seen two days around ovulation time. We concluded that color Doppler ultrasound performed once daily was more accurate in predicting the preovulatory LH peak than B-mode ultrasound and vaginal cytology..https://bvmj.journals.ekb.eg/article_248908_8d608444f827157e0c81d4afe1bbdf20.pdf