The Effect of Recipient Holstein heifers’ Corpus Luteum Type, Diameter and Secretory Potential on their Pregnancy Rate during Routine ET program in Egypt

Oshba, MR; Sosa, G; Nossier, M; Fadel, M; El-Raey, M

doi:10.21608/bvmj.2019.103399

The Effect of Recipient Holstein heifers’ Corpus Luteum Type, Diameter and Secretory Potential on their Pregnancy Rate during Routine ET program in Egypt

Document Type : Original Article

Authors

¹ Diagnostic Imaging and Endoscopy Unite, Animal Reproduction Research Institute, Al Haram.

² Department of Theriogenology, Faculty of Veterinary Medicine, Benha University, P.O:

³ Department of Theriogenology, Faculty of Veterinary Medicine, Alexandria University

10.21608/bvmj.2019.103399

Abstract

The objective: of this study was to find the correlation between the pregnancy rate of Holstein recipient heifers and their corpus luteum type, diameter and its secretary competence at the day of embryo transfer. Methods: A total of (92) recipients were selected after estrus synchronization by prostaglandin. Fresh embryos were collected from (12) donors. Corpus luteum type and diameter were determined at transfer date using transrectal ultrasound. Serum samples were collected at transfer day (7^th day post heat/luteal phase) for progesterone and Estradiol-17ß assessment. Results: Current study revealed non-significant difference between pregnant and non-pregnant recipients concerning CL diameters; regardless CL type either compact (24±0.83 and 24±0.58mm, respectively) or cavitary (26±1.1 and 27±1.4mm, respectively); however there was significant difference (P<0.05) between compact and cavitary CL diameters in non-pregnant recipient. Concerning, the relationship between CL type and pregnancy rate: thirteen pregnancies (39.39^* %) had cavitary CL; whereas, twelve pregnancies (20.34%) had compact CL. Cavitary CL secreted progesterone (5.9 ± 1.7ng/ml) and estradiol (43±1.9 pg/ml). Compact CL recorded low level of progesterone (4.4±0.96 ng/ml), and high estradiol concentration (49±1.4 pg/ml). The percentage of cavitary CL in Holstein breed was (35.87%) versus (64.13%) for compact ones. Ratio of luteal cavity/luteal tissue was (0.48%) for Holstein recipients heifers. Conclusion: Corpus luteum type not diameter was a decisive factor in recipient selection. Luteal phase estradiol concentration was a crucial in recipient selection and should be at the lowest concentration. The percentage of cavitary CL for Holstein recipients was (35.87%), and the ratio of luteal cavity to the luteal tissue was (0.48%).

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