Anti-inflammatory and antioxidant effects of pomegranate on acetic acid-induced ulcerative colitis in rats

Document Type : Original Article

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Clinical Pathology dept., Faculty of Vterinary Medicine, Benha University.

Abstract

The present study was designed to investigate the protective effect of pomegranate extract on some blood and antioxidant parameters in addition to interleukin-1 (IL-1) and inducible nitric oxide (iNOS) gene expression of colon tissue in acetic acid-induced ulcerative colitis in rats. Forty rats were divided into four equal groups. Group I (c) group (control normal group) received no drugs. Group II (AA) group (Acetic acid induced ulcerative colitis group) rats were administrated with 2 ml of acetic acid (AA) 4% intracolonic as a single dose. Group III (PGFE) group (Pomegranate group) rats were administrated with pomegranate orally (500 mg/kg, b.wt/day for 14 days. Group VI (PGFE+AA) group (PGFE protected plus AA 4% induced ulcerative colitis group) rats were administrated with PGFE orally (500 mg/kg, b.wt/day for 14 days and at the 11th day was administrated intracolonic as a single dose with 2 ml of AA 4%. The obtained results showed that a significant decrease in neutrophils to lymphocytes ratio (N/LR) and increase in lymphocytes to monocytes ratio (L/MR) in (PGFE+AA) group when compared with (AA) group. Meanwhile, malondialdehyde (MDA) and nitric oxide (NO) were significantly decreased with significant down-regulation in IL-1ß and iNOS gene expression levels were obtained in colon tissue of (PGFE+AA) group when compared with (AA) group. The results revealed that pomegranate has protective, anti-inflammatory and antioxidant effects in experimental model of ulcerative colitis in induced rats.
 

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Benha Veterinary Medical Journal
Volume 36, Issue 1
March 2019
Pages 119-132

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