Comparison of the efficacy of local and imported inactivated combined H9-ND virus vaccines in protection of broiler flocks against H9N2 infection in Egypt

Document Type : Original Article

Authors

1 poultry and rabbit disease , faculty of veterinary medicin Aswan university

2 assistant researcher central laboratory for evaluation of veterinary biologic , Abasia , Egypt

Abstract

The low pathogenic avian influenza becomes endemic in Egypt since its first record in 2011. It affected all the chicken sectors. There are some commercial vaccines either imported or local vaccine. This study conduct to compare the effectiveness of two H9ND vaccines, one imported and the other local, through two experiment the first in the laboratory using 150 one day old broiler chicks divided into 3 groups , the first two groups were vaccinated by the imported and local vaccine, respectively at one day old, the third group kept as negative control , the antibodies against H9N2 virus were measured by HI test at 14,21 and 28 day old , at 21 day old each group were divided to two group the 1st kept unchallenged, the 2nd challenged with H9N2 virus using the circulating local strain , in the chicken isolators ,cloacal and tracheal swaps were taken at 2,4and 6 days post challenge to detect the shedding virus using real time RT- PCR test. The second experiment were in a commercial broiler flock contain 432,000 chickens placed in 16 pens. The broiler chickens divided into 2 groups, each group took a different commercial H9ND vaccine at 1st day, the humeral immunity measured, at 14,21and 28 day old.
. The results showed that the locally produced vaccine provide significant higher immune response in both lab. and field experiment ,on the other side the virus shedding is stopped early in the group vaccinated by local vaccine.

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