Molecular detection of antibiotic resistant bla gene in B. cereus isolated from meat products

Document Type : Original Article


1 Bacteriology, Immunology, and Mycology, Faculty of Veterinary Medicine, Benha University, Benha, Egypt

2 Animal Health research institute, Shebin El koom branch

3 animal health research institute


In this study the incidence of Bacillus cereus (B. cereus) and antibiotic sensitivity test was undertaken. A total of one hundred random samples of meat products of rice kofta, kobeba, chicken pane and chicken nuggets (25 of each) were collected from different supermarkets at different times in Menofiea and Kalyobia governorates, Egypt and tested for occurrence of Bacillus group. Bacillus cereus were detected in 24%, 12%, 20% and 10% of kobiba–shami , rice kofta, chicken pane and chicken nuggets ,respectively . Bacillus mycoids failed to be isolated from kobiba–shami and isolated from 4%, 16% and 4% of rice kofta, chicken pane and chicken nuggets, respectively . Also, B. thuringenesis failed to be isolated from kobiba–shami and rice kofta while it isolated from 8% of both chicken pane and chicken nuggets. Fourteen strains of B. cereus were examined for antimicrobial susceptibility testing. The foremost common drug resistance was to penicillin, amoxicillin and amoxicillin+clavulanic (100% for each). On the other hand, B. cereus was completely prone to vancomycin and gentamycin (100% for each).Using PCR, all tested B. cereus isolates harbored bla gene.


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