A potent chemopreventive effect of diethyl ether extract of lotus peregrinus L. on apoptosis associated proteins and oxidative stress markers in mammary tumor –induced in rats

Hussein, Samy; Abdel-Aal, Samir; Shalabia, Emam

doi:10.21608/bvmj.2019.114675

A potent chemopreventive effect of diethyl ether extract of lotus peregrinus L. on apoptosis associated proteins and oxidative stress markers in mammary tumor –induced in rats

Document Type : Original Article

Authors

¹ Department of Biochemistry, Faculty of Vet. Med., Benha University, Egypt.

² Department of Animal Hygiene, Behavior and management, Faculty of Vet. Med. Moshtohor, Benha University, Egypt

³ Departments of Medicinal and aromatic plants, Desert Research Center, Matriya-Cairo; Egypt.

10.21608/bvmj.2019.114675

Abstract

extract and to determine whether the diethyl ether extract of Lotus peregrinus L. exerts chemo-protected effect by stimulating apoptosis during medroxyprogesterone acetate (MPA)-DMBA mammary carcinogenesis using the expression of the apoptosis-associated proteins Bax and caspases 9; as well as improvement of oxidative status of the mammary tumor tissue. Sixty female wistar rats (52 days old) were divided into four equal groups. Group I (normal group): rats administered distilled water. Group II (carcinogenic group); on day 0, wistar rats were given DMBA (2.5 mg/ rat; orally) followed by second dose DMBA (2.5 mg/ rat; orally) on day 15, 90-day timed-release pellets containing 25 mg MPA were implanted’ into rats on day 30. Group III (diethyl ether protected), on day 0, wistar rats were given DMBA (2.5 mg/ rat; orally) followed by second dose DMBA (2.5 mg/ rat; orally) on day 15, on day 26 rats were administered diethyl ether (50 mg /kg. b. wt.; orally), 90-day timed-release pellets containing 25 mg MPA were implanted into rats on day 30. Group IV (diethyl ether treated group); given diethyl ether (50 mg /kg. b. wt.; orally) after induction of mammary cancer. Our findings showed the positive treatment of mammary tumor and improved oxidative status of the tissue was evident by increase in the levels of GSH, Catalase and decreased levels of serum MDA levels which in turn is an indicator of reduced oxidative stress of the tissue. Therefore, this study suggests that diethyl ether extract of Lotus peregrinus can be useful for the treatment as well as improvement of oxidative status of the mammary tumor tissue .Also, was more effective in inhibiting DMBA-MPA induced mammary tumors and modulating the expression of apoptosis associated proteins.

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