Comparative potentiality study of three different vero cell culture systems for production of PPR Vaccine

Document Type : Original Article

Authors

1 Department of Virology, Faculty of Veterinary Medicine, Benha University

2 Veterinary serum and vaccine Research institute, Abbasia, cairo.

Abstract

Peste des petits ruminants (PPR) is considered one of the most dangerous viral diseases of small ruminants in Africa and Asian countries. The disease control is based on vaccination of susceptible animals with an attenuated PPR virus strain (Nigeria 75/1) propagated in monolayers of Vero cells using stationery and roller flasks. This study for constructing alternative method for production of PPR vaccinal strain through propagation of PPR virus (75/l) on Vero cell culture supported on cytodex-three microcarrier beads using spinner stirring flasks and compare the recorded results with that obtained from stationary flasks (175cm2) and Roller Bottles. All cultures were propagated under the same conditions (media, pH, time of incubation) and infected with the same multiplicity of infection by PPR vaccinal virus. Samples were obtained daily for successive five days from all cultures. The PPR virus titers were 12 log10 TCID50, 5 log10 TCID50 and 6 log10 TCID50 on microcarriers culture, stationary system and roller system respectively after six days post infection. These results provide further insights to apply microcarriers cell culture technology in production of PPR vaccine.
 

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Benha Veterinary Medical Journal
Volume 34, Issue 1
March 2018
Pages 466-473

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