Optimizing culture conditions for increasing production of vero cell

Document Type : Original Article

Authors

1 Department of Virology, Faculty of Veterinary Medicine, Benha University

2 Veterinary Serum and Vaccine Research Institute, Abbasia, cairo.

Abstract

The key to achieving maximum yields from microcarrier cultures is by using the optimization factors as replenishment of vero cell culture media during growth, the inoculation density on the proportion of microcarriers bearing cells, stirring speed on the growth of vero cell on cytodex 3, culture volume and headspace on cell yield from closed microcarrier system, various culture media, various types of the serum, control of PH, microcarrier concentration and modified initial culture procedure. The result reveled that the higher cell yield obtained after replenishment of culture media every three day, the optimum concentration of microcarrier is to be 3mg cytodex/ml, the optimum inoculation density 15x106 cells/mg cytodex microcarrier is required, the higher cell yield obtained by using (medium 199) containing high amount of amino acid at low density of the cell higher than Dulbecco’s modification of Eagles’s medium (DME) in higher density of the cell, fetal calf serum gave higher cell yield in the first three days in the culture, 60 rpm was the optimum stirring speed to get higher cell yield, addition of 10 – 25 mμ Hepes helped in maintaining the PH around 7.3-7.4 this gave higher cell yield, reduced the head space not more than half full of the culture give a higher result and the modified in initial culture procedure during the growth culture give higher results. The obtained result maximizing the yield of the vero cell production on acytodex-3 microcarrier culture system.
 

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Benha Veterinary Medical Journal
Volume 34, Issue 1
March 2018
Pages 474-482

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