Assessment of Antibactrial Effect of Different Extracts of Thymus Vulgaris against Clostridium Perfringens in Chicken

Document Type : Original Article

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1 Department of Bacteriology, Immunology and Mycology, Faculty of Veterinary Medicine, Benha University

2 Department of Bacteriology, Immunology and Mycology Faculty of Veterinary Medicine, Zagazig University

Abstract

Necrotic enteritis in chicken is caused by the Clostridium perfringens, which have been found on almost chickensand for decades antimicrobial therapy was the only strategy to control it. However the misuse of antimicrobials linked to many impacts like reduced susceptibility of C. perfringens strains and cause cross-resistance to antimicrobial agents. Thisworkdemonstrates that, the uses of herbal extracts with antibacterial effectsconsider as an effective way to prevent and reducethe Necrotic enteritis.The present study determines antimicrobial activities of methanolic, ethanolic and watery extracts of thymus vulguarisagainst Clostridium Perfringenstype Ain chicken.The antibacterial activity was assessed through measuring sensitivity of isolated strains to different extracts by agar diffusiontechniqueand minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC)by using the broth macrodilution method. Thymus vulgaris different extracts consider as effective anticlostridial herbal agent. The result of agar well diffusion assayand broth macrodilution methodshowed that, the tested strains were more sensitive to theethanolic and methanolic extracts which give the lowest (MIC) and (MBC) (32, 16 respectively) than the watery extracts (512, 256 respectively) .

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