Clinicopathological Effect of Induced Pluripotent stem cells and Hormonal Therapy on Premature Ovarian Failure

Document Type : Original Article

Authors

1 Clinical pathology department,faculty of veterinary medicine,benha university

2 Histology and Cell Biology, Faculty of Medicine, Benha University.

3 Pathology Department, Animal Health Research Institute

4 Clinical Pathology Department, Faculty of Veterinary Medicine, Benha University

Abstract

A b s t r a c t
This experiment was designed to investigate the roles of induced pluripotent stem cells and hormonal therapy on experimentally-induced ovarian ablation (OA) by evaluating of hormonal, molecular, histopathological and immunohistochemical examinations. Fifty female rats classified into five main groups. Group (I):10 rats as control normal group. Group II (OA): 10 rats injecteded with (3 mg/kg) of DOX and (50 mg) CYP dissolved in clean physiological saline I/P once week for 5 weeks. Group (III): 10 rats go through chemoablasion, after that, rats were injected with 5 IU of PMSG single subcutaneous (S/C) injection. Group (IV): 10 rats undergo chemoablasion, after that induced pluripotent stem cells (iPSc) were injected intravenous (I/V) single injection for 2 months. Group (V): 10 rats undergo chemoablasion then iPSc were injected I/V combined with single S/C injection of 5 IU PMSG. Results revealed that rats injected with doxorubicin and cyclophosphamide showed a significant increase in FSH, LH levels while asignificant decrease in E2, PCNA, Oct4, Stra8 and Runx2 when compared with control (-ve) group. On the other hand, rats treated with PMSG or IPSc only or PMSG and IPSc revealed that there were increase in E2 level, PCNA, OCT4, Stra8 and Runx2 while there is decrease in FSH, LH levels when compared with chemoablated control group. Also, ovarian sections of chemoablated group showed degenerated surface epithelia, loss of secondary, and antral follicles. ovarian sections of group treated with PMSG and iPSc showed well observed primordial, primary and secondary follicles.

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