Preparation and evaluation of Necrotic enteritis (NE) vaccine using alpha toxin with different adjuvants in broilers.

Document Type : Original Article


1 Animal health institute, Tanta branch, egypt

2 Department of rabbits and birds diseases, Faculty of Veterinary Medicine, Benha University.

3 Veterinary Serum and Vaccine Research Institute, Abbasia, Cairo.

4 Animal Health Institute, Tanta branch, Egypt


In the present study we used one hundred and twenty-five broiler chicks (Arbo) one- day old used as experimental birds, the experimental chicks were divided into five groups each of twenty-five chicks. The first group kept as control, the 2nd group was non-vaccinated and challenged, the 3rd group was infected with Emeria tenella at 15th days of age and challenged, the 4th group was vaccinated and challenged, the 5th group was vaccinated and challenged. The vaccinated chicks in group 4 was vaccinated by locally prepared NE vaccine adjuvinated with Aluminum hydroxide by dose of 0.5ml subcutaneously at age of seven days and boostered at age of eighteen days. While the chicks of 5th group were vaccinated by locally prepared NE vaccine adjuvinated with Montanide oil. The challenged groups (2, 3, 4 and 5) were challenged at age of 27, 28 and 29 days with C. perfringes type A whole culture by dose of 3ml contains 109 Cfu. Serum samples were collected after challenge at thirty-five days. The results showed that higher immune response, lower mortality rate, increase in body weight and low feed conversion rate in chicks of group 5 compared to chicks of groups (1, 2, 3 and 4).


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