Preparation of a house ELISA kit for detecting Peste des petits ruminants Virus (PPRV) antibodies

Document Type : Original Article

Authors

1 ELISA and virus strains bank Research unit, Animal Health Research Institute, Agriculture Research center,Giza, Egypt

2 elisa and viral strains bank research unit, animal health research institute, Agriculture research center, Dokki,Giza, Egypt

Abstract

Peste des petits ruminants (PPR) is a viral disease that affects small ruminants characterized by fever, highly contagious, and economically significant. For diagnosis of the disease, effective diagnostic techniques are essential. The immunological diagnostic procedure enzyme linked immunosorbent assay (ELISA) is used to identify antibodies or antigens. Using semi-purified antigen prepared by Peste des petits ruminants virus (PPRV) Egypt/77 grown in Vero cell culture, an indirect ELISA based on polyclonal antibodies was developed to detect PPR antibodies in goat and sheep serum samples. When using 1:40 serum dilution and 1:80 antigen dilutions, the antibody detection effect is better. Depended on the presence of known PPR antibody-negative control serum samples in the test, a cut-off value twice the average value of the negative control was obtained. Indirect ELISA and competitive ELISA were used to test 130 serum samples from sheep and goats (105 sheep samples and 25 goat samples). Indirect ELISA revealed 71 positive samples and c-ELISA revealed 79 positive samples out of 130 serum samples analysed. When compared to the competitive ELISA, the indirect ELISA shows 83 percent specificity and 97.1 percent sensitivity. These findings show that indirect ELISA can be used instead of competitive ELISA for PPR antibody serological surveys in small ruminants.

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Benha Veterinary Medical Journal
Volume 41, Issue 1
October 2021
Pages 24-28

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