A Recent Non cytopathogenic bovine viral diarrhea virus detected by immunofluorescence and reverse transcriptase polymerase chain reaction

Abdelkareem, Samia; El-Habbaa, Ayman Said; Aboezz, Zeinab R.A.; ElNahas, Ehab

doi:10.21608/bvmj.2021.79012.1427

A Recent Non cytopathogenic bovine viral diarrhea virus detected by immunofluorescence and reverse transcriptase polymerase chain reaction

Document Type : Original Article

Authors

¹ Viral Diseases Research Unit, Animal Reproduction Research Institute (ARRI), Agricultural Research Center (ARC), Giza, Egypt

² Virology department, faculty of veterinary medicine, Benha university, Moshtohor, Egypt

³ Virology department, faculty of vet medicine, Benha university.

⁴ Virology Department, Faculty of Veterinary Medicine, Benha University, Moshtohor 13736, Kalubia, Egypt

10.21608/bvmj.2021.79012.1427

Abstract

In the present study, a non-cytopathogenic bovine viral diarrhea virus (BVDV) field strains from bovine semen samples were detected by immunofluorescence (IF) and reverse transcriptase polymerase chain reaction (RT-PCR). Two out of five semen samples tested positive for the presence of BVDV antigen employing antigen capture ELISA kits. The two semen samples were injected in Madin-Darby bovine kidney (MDBK) cells in a trial for BVDV isolation. The chosen two samples were not cytopathic and subjected for further identification and genotyping using one step reverse transcriptase polymerase chain reaction (RT-PCR) assay. The isolated BVDV field strains from semen were found to be BVDV genotype 1.

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