Molecular characterization of Salmonella serovars isolated from Chicken

Document Type : Original Article

Authors

1 Department of Bacteriology, Immunology and Mycology, Faculty of Veterinary Medicine Benha University, Egypt.

2 Department of Bacteriology, Immunology and Mycology, Faculty of Veterinary Medicine, Benha University

3 Animal Health Research Institute, Serology Unit, Giza, Egypt

Abstract

A total of one hundred diseased chicken (100 samples of each of Liver , Gall bladder and Intestine content) suffering from diarrhea were collected from different broiler private farms in Giza governorate, Egypt. Samples were collected in a sterile container in an ice bag and then transferred aseptically to the laboratory for conventional bacteriological analysis
Bacteriological examination revealed that Salmonella from liver were (2.66%), Gall bladder ( 3% ) and Intestine ( 1.66 % ).
The prevalence rate to Salmonella was (7.66%). Antibiogram pattern was applied and isolates exhibit resistance against Rifampicin, Amoxycillin and Doxycyclin and highly sensitivity to Streptomycin with (100%) variable results were recorded with the remaining test antibiotic molecular characterizations of B-Lactamase resistance genes in salmonella serovars gave variable results.
A PCR based assay was developed to detect the prevalence of Salmonella in samples and to and chromosome-borne virulence genes (blaTEM ,blaSHV and blaCTX ). The aims of the current study wasbacteriological and molecular studies on Salmonella species isolated from poultry farms

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Benha Veterinary Medical Journal
Volume 41, Issue 1
October 2021
Pages 94-98

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