Some Biochemical Changes and Role of Micro RNA 34 a-5p and Micro RNA 365a-5p as A Diagnostic Marker in Chronic Hepatitis C (HCV).

Document Type : Original Article


1 Department of Biochemistry, Faculty of Veterinary Medicine. Benha University

2 Department of Internal Medicine, Faculty of Medicine, Benha University.

3 Department of Clinical Biochemistry, Faculty of Medicine, Benha University.


Hepatitis C virus caused acute and chronic hepatitis. Micro RNA could be used as disease biomarkers. This study aimed to investigate Micro RNA34a-5p , Micro RNA 365a-5p and biochemical analysis as an accurate diagnostic biochemical marker for chronic Hepatitis C Virus (HCV).
The present study included 17 individuals had chronic liver disease, who admitted to Banha University Hospital. After investigating 5 patients from all patients, were found to be positive PCR chronic HCV. 10 control negative serum samples were collected from the control healthy group. Biochemical analysis was done for investigating liver function parameters (ALT, AST, TBIL, DBIL, GGT, ALP and ALB), and hematological analysis including TLC, HB concentration and platelet count. AFP as a tumor marker indicator. RNA was extracted from serum samples for gene expression of microRNA34 a-5p and microRNA 365a-5p. Results: HCV group showed a significant increase liver function parameters (ALT, AST, TBIL, DBIL, GGT, ALP) while there was a significant reduction in the albumin levels. Moreover, there was a significant decrease in TLC with non-significant decrease in the HB concentration and the platelet count. AFP concentration in HCV group was significantly increased compared to the control group. HCV group revealed a significant increase (8.98 ± 0.34) in mRNA34, with a significant decrease in mRNA365 (0.25 ± 0.03). We concluded that mRNA34a-5p and mRNA365a-5p expressions in addition to different biochemical analysis can be used as an accurate diagnostic marker for HCV diagnosis


Main Subjects