The Possible Impacts of Rosemary and Hops Ethanolic Extracts on Hepatocellular Carcinoma Experimentally Induced in Rats

Document Type : Original Article


1 Biochemistry and Molecular Biology Department; Faculty of Veterinary Medicine, Benha University

2 Animal Wealth Development Department (genetics and genetic engineering), Faculty of Veterinary Medicine, Benha University, Egypt

3 Biochemistry and Molecular Biology Department, Faculty of Veterinary Medicine, Benha University


Hepatocellular carcinoma (HCC) constitutes a major health hazard in Egypt and globally for its high prevalence. This study is designed to evaluate the anti-carcinogenic effect of rosemary and hops on hepatocellular carcinoma experimentally induced in rats using cisplatin as standard chemotherapy. Fifty rats were randomly separated into five equal groups: control group: didn't receive any treatment; groups (2-5) received three doses of diethyl nitrosamine (DEN) 200 mg/kg b.wt. by intraperitoneal injection with two weeks intervals then DEN + cisplatin group injected intraperitoneally with 1.5 mg/kg b.wt Cisplatin; DEN + rosemary group orally administrated 200 mg/kg b.wt. rosemary ethanolic extract; DEN + hops group administrated 150 mg/kg b.wt. hops ethanolic extract orally. Groups 3-5 received their treatments for 4 weeks. Blood samples were collected at the end of 10th week of experiment for biochemical evaluation of hepatic functions. Results of this study revealed that DEN markedly increased alpha-fetoprotein (AFP) and all hepatic enzyme activities evaluated and decreased serum albumin and total protein. On the other hand, cisplatin, rosemary, and hops mitigated to a large extent all the biochemical alterations caused by DEN in different degrees. Notably, rosemary extract was more effective in preserving hepatic function than hops when compared to cisplatin. Our results indicate that rosemary and hops may have anti-carcinogenic potentials compared to cisplatin which provides natural, safe, feasible alternative to chemotherapy.


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