Phenotypic and Molecular characterization of Candida albicans isolated from milk and buccal cavity of child

Document Type : Original Article

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microbology and chemical fac. of science

Abstract

An opportunistic pathogen called Candida albicans induces both noninvasive and invasive infections. So, this study was aimed to estimate the occurrence and phenotypic characterization of C. albicans in 175 specimens (150 samples from milk and 25 buccal cavity swabs of candidiasis patients) collected from different dairy shops and hospitals at Kaliobia Governorate Egypt, beside genotypic identification of some isolated strains. This study cleared that, C. albicans isolates were isolated from 29 (16.6%) samples, 11 (6.3%) from milk samples and 18 (10.3%) from buccal cavity swabs. Moreover, all C. albicans isolates were sensitive for antifungals studied with various percentages, and they were highly sensitive to Fluconazole followed by voriconazole then itraconazole ; amphotericin-B ; nystatin and finally clotrimazole .In addition, PCR outcomes demonstrated that, Its and Als1gene were detected in all seven studied C. albicans strains giving products of 109 bp. and 318 bp., respectively. So, this study concluded that, marked milk could serve as a pathogenic C. albicans' transmission vehicle; it is one of the most vital etiologies of oral candidiasis (thrush) and could be controlled by using fluconazole, voriconazole, nystatin, clotrimazole, itraconazole and amphotericin-B. Also, Itsand Als1 genes are good tools for C. albicans isolates' identification.

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Benha Veterinary Medical Journal
Volume 43, Issue 2
January 2023
Pages 75-79

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