Hepatoprotective Effect of Allopurinol and Garlic Extract against Experimentally – Induced Hepatotoxicity

Document Type : Original Article

Authors

Dept. of Clinical Pathology, Faculty of Vet. Med., Benha University, Egypt

Abstract

The present study was designed to investigate the hepatoprotective and antioxidant effects of
allopurinol and garlic extract against acetaminophen and ethanol induced hepatotoxicity and
oxidative stress in rats. Animals were divided into four groups .Group 1 was used as a control.
Group2 was administered paracetamol plus ethanol orally for 14 days beginning from day 15 – 28
of the experiment. Group 3 was administered allopurinol orally for 28 days, and from day 15 – 28
of the experiment rats were given paracetamol plus ethanol orally till the end of the experiment.
Group 4 was administered garlic extract orally for 28 days, and from day 15– 28 of experiment rats
given paracetamol orally plus ethanol till the end of the experiment. Serum was separated and used
directly for determination of alanine aminotransferase(ALT), aspartate aminotransferase(AST),
albumin, alkaline phosphatase(ALP),bilirubin(BIL) and Tumor necrosis factor –alfa(TNF).Liver
samples were taken for histopathological examination, liver malondiadehyde(MDA), Glutathione
peroxidase(GPX)and nitric oxide(NO).The result revealed that in group 2 there were significant
elevations of alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, bilirubin,
Tumor necrosis factor–alfa and liver malondiadehyde and nitricoxide. Furthermore, there were
significant decreases in Glutathione peroxidase and albumin compared with control group.
Meanwhile, administration of Allopurinol and garlic resulted in significant decrease in all elevated
mentioned parameter and increase in Glutathione peroxidase and albumin compared with
hepatotoxicity group. Therefore it could be concluded that allopurinol and garlic as strong
hepatoprotective and antioxidant effects

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