In vitro maturation (IVM) of camel oocytes using different maturation media

Document Type : Original Article

Authors

1 Theriogenology, Benha university, Benha, Egypt

2 Theriogenology department, Faculty of Veterinary Medicine, Benha University

3 Theriogenology Department, Faculty of Veterinary Medicine, Benha University, Egypt.

4 Theriogenology department -benha university- faculty of veterinary medicine.

Abstract

The aim of the present study is to evaluate the in vitro maturation rate of camel oocytes using different maturation media. Ovaries of 25 she-camel were collected during the breeding season (from December to April). Ovaries were transferred to the lab in a thermal flask containing warm saline solution within 2 hrs. after slaughtering. Oocytes were collected using 2 techniques; aspiration of graafian follicles and slicing of the ovaries. Depending on the morphological quality, the collected oocytes were selected and cultured on different types of maturation media for 30-40 hours at 38.5˚c, 5% CO2, and maximum humidity (95-96%). The obtained results revealed that the slicing technique produced a higher oocyte recovery rate compared to the aspiration technique. In addition, the maturation media composition mainly the FSH affects the in vitro maturation rate of camel oocytes. It could be concluded that the media modification used in oocytes maturation might be one of the factors that improve the rate of oocyte maturation.

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