Hepatoprotective effect of flaxseed oil and alpha lipoic acid against cisplatin-induced oxidative stress and apoptosis in rats

Document Type : Original Article


Department of pharmacology, Faculty of Veterinary Medicine, Benha University, 13736 Moshtohor, Toukh, Qaliobiya, Egypt


Cisplatin (CP) is a widely used chemotherapy agent. The study's goal was to assess the antioxidant and antiapoptoptic effects of flaxseed oil (FXO) and/or alpha lipoic acid (ALA) against cisplatin-induced hepatic toxicity. Seven groups (7 rats/group) were assigned; control (saline) group, FXO group; ALA; CP toxic group; CP+FXO, CP+ALA, and CP+FXO+ALA groups. Saline, FXO, and ALA were administered for 21 days. After CP injection, serum AST and ALT activities were decreased and serum albumin level was deceased. Malondialdehyde (MDA) in the liver tissues was significantly increased and reduced glutathione and catalase levels were decreased. Additionally, CP toxicity induced hepatocellular damage and elevation of caspase-3 expression in the liver tissues. Furthermore, rats intoxicated with CP and given FXO and ALA alone or in combination showed significant reductions in oxidative stress indicators and biochemical parameters, as well as improved hepatic tissue architecture, when compared to the CP group. So, concurrent administration of FXO and/or ALA during CP therapy is advisable.


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