Development of Simple Multiplex Real-Time PCR Assays for Some Probiotics Detection

Document Type : Original Article


1 Department of Biochemistry, Faculty of Veterinary Medicine, Benha University, Egypt

2 Department of Biochemistry, Faculty of Veterinary Medicine, Cairo University, Egypt


The main objective of this study to investigate the development of simple multiplex real time for some probiotics
detection. The probiotics defined as Live microorganisms when administered in adequate amounts confer a health benefit
on the host. Most commonly used probiotics are Lactic acid bacteria (LAB) and bifido bacteria. There are other examples
of species used as probiotics (certain yeasts and bacilli). Probiotic supplements are popular now a day. From the beginning
of 2000, research on them has increased remarkably. The beneficial effects of probiotics are now day’s widely studied in
treatment of many prevailing diseases. In this study, we present the development of molecular methods. Conventional
multiplex PCR and SYBR Green based real time PCR assays were performed using genus and species specific primers.