Experimental pathological studies on the ability of Stem cells for regeneration of hepatocytes in rats

Document Type : Original Article


1 Pathology department of Animal medicine, Faculty of Veterinary Medicine, Benha University

2 Department of experimental embryology and stem cells technology. Faculty of Science Al-Azhar University


Stem cell therapy is an important catigory of regenerative medicine has the potential to provide a valuable adjunct and
alternative to liver transplantation with treatment of liver fibrosis and cirrhosis. In this approch, 70 male albino rats were
randomly divided into 4 groups: Group 1: Control group (20 rats), Group 2: TAA-treated group (20 rats), Group 3:
Mesenchymal stem cells treated group (15 rats) and Group 4: TAA- Mesenchymal stem cell –treated group (15 rats). Our
results showed that TAA is significantly increase the activities of ALT, AST, and ALP and reduced the levels of total
protein, albumin and globulins in the serum. Moreover, TAA successfully induced liver cirrhosis characterized by coarse
nodular liver surface with severe fibrous connective tissue proliferation led to pseudolobulation of hepatic parenchyma.
Meanwhile, after treatment with Mesenchymal stem cells (MSCs) the liver of the rats was grossly nearly normal and
microscopically thin strands of fibrous connective tissue in between the hepatic lobules with mild degenerative and
necrotic changes and significant improvement in the liver blood parameters. These findings concluded that MSCs have
ameliorative effect on chemically induced liver cirrhosis through their regenerative capacity and antifibrotic effect.