Protective role of new nanocomposite natural product (basic nano-curcumin) against breast carcinoma

Document Type : Original Article

Authors

1 Clinical Biochemistry Department - Faculty of Veterinary Medicine- Benha University

2 Medical and Aromatic Plants Department- Desert Research Center

3 Applied Chemistry Department- Egyptian Petroleum Research Institute.

4 Medical and Aromatic Plants Department- Desert Research Center.

Abstract

Present study focused on evaluation of the effect of national nancomposite product on serum biochemical profile and
programmed cell death (apoptosis) by using Annexin V-FITC and PI test of breast carcinoma female rats. Basic nanocurcumin
was tested for its protective effect on breast cancer female rats (n= 30) divided in two main groups (NTBR) and
(TBR) which in turn classified into (TBR- Control Positive and TBR- basic nano- curcumin protective groups). Basic
nano-curcumin was formulated and tested for both IC50 (IC50 = 1.0 μg) and LD5o (35 mg/kg by wt.) by using in vitro and
in vivo techniques, respectively. Breast cells apoptosis was determined by Annexin V-FITC, which is a fluorescent probe
binds to phosphotidylserine (PS) in a Ca 2+ dependent pathway. Breast Cells were stained with Annexin V and PI. The
apoptotic cells [Annexin (+) and PI (-)] were differentiated from necrotic cells [Annexin (-) and PI (+)] using flow
cytometry technique. The anti-proliferative effect of basic nano-curcumin appeared much earlier due to its nano form.
Also, Liver [ALT (40U/l), AST (85 U/l)] and kidney [BUN (19 mg/dl), Creatinine (0.5 mg/dl)] functions were decreased
within normal ranges

Keywords

Benha Veterinary Medical Journal
Volume 33, Issue 2
December 2017
Pages 107-116

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