Evaluating humoral immune response of sheep vaccinated with inactivated RVF virus vaccine using oil nanoparticles adjuvant in comparison with aluminum hydroxide gel

Document Type : Original Article

Authors

1 Departments of virology, Faculty of Veterinary Medicine, Benha University, Egypt

2 Veterinary Serum and Vaccine Research Institute, Abbassia, Cairo.

Abstract

In the present study an inactivated tissue culture adapted RVF virus vaccine was prepared using
Montanide oil IMS 1313 nanoparticles as adjuvant then evaluated in sheep in comparison to the local RVF
virus vaccine with aluminum hydroxide gel adjuvant using serum neutralization test (SNT) and Enzyme
Linked Immuno-Sorbent Assay (ELISA). The prepared vaccine was sterile and safe inducing no systemic or
local clinical signs in sheep. Results indicated that the prepared nanoparticles oil based vaccine induced a
protective neutralizing serum antibody titer from the 2nd week post vaccination (WPV), reached the highest
level at the 3rd month post vaccination (MPV) and persisted in protective level until the for 9th MPV, while
aluminum hydroxide gel based vaccine appeared in protective level at 2nd WPV, reached the highest level at
the 2nd MPV and persisted in protective level till the 7th MPV. These results were confirmed by using ELISA.
These results demonstrated the potentiality of the prepared nanoparticles oil based vaccine compared to the
locally produced aluminum hydroxide gel based vaccine reflected by greater immunogenicity and longer
duration of immunity.

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