Identification and characterization of klebsiella pneumonia isolated from farm animals and their biofilm production estimation

Klebsiella pneumoniae considered one of the most commensal bacteria which cause Economical problems in farm animals as causing mastitis and respiratory disorders in ruminants and neonatal septisemia in calves. Antibiotic are commonly used for disease control in animals and human. Multidrug resistant (MDR) K.pneumoniae strain show resistance to more than one antibiotic. In the present study K.pneumoniae were isolated and identified from different clinical samples(nasal swabs, milk sample, anal and vaginal swabs). a total of 293 sample from 190 sample from clinically diseased cattle, 60 from clinically diseased buffaloes and 43 from clinically diseased calf. Isolation rate were recorded that 20 /190 samples taken from clinically diseased cattle(10.5%), 5 /60 samples from clinically diseased buffaloes(8.33%) and 5 /43 samples from calfs (11.6%). Klebsiella was isolated between samples type as 24 /233 nasal swabs, 4/45 mastitic milk sample and 2 /10 anal swabs sample with percentage of (10,3%, 8,8 and 20%) respectivly The anti microbial susceptability test were done for 30 isolates of klebsiella using disc diffusion method, the results showed that the highest resistanc to ampicillin (100%) followed by chloramohinicol( 76.7%) and the highest sensitivity to gentamycin (100%) followed by nalidexic(73.33%), ciprofluxacin and erythromycin the same by (76.7%) followed by streptomycin (53.4%). The biofilm formation ability of k. pneumoniae protect bacteria from immune system of the host. In the present study of biofim formation module indicated that all strains were biofilm formed strains among them, 11 (36.66%), 12 (40%), and 7 (23,33%) were strong, moderate, and weak biofilm producers, respectively.