Genetic relatedness between recent field isolate strain of LSDV and other field and vaccinal strains based on GPCR, ORF095 and ORF103 genes in Egypt 2020

Abdelsalam, Adel Elsayed; El-Habbaa, Ayman Said; Abo Hatab, Eman Mohamed; Sharawy, Saad

doi:10.21608/bvmj.2023.206881.1653

Genetic relatedness between recent field isolate strain of LSDV and other field and vaccinal strains based on GPCR, ORF095 and ORF103 genes in Egypt 2020

Document Type : Original Article

Authors

¹ 1Department of Virology, Faculty of Veterinary Medicine, Benha University, Moshtoher, Benha, Egypt 2Virology Department, Animal Health Research Institute, Agricultural Research Center, Giza, Egypt

² Virology Department, Faculty of Veterinary Medicine, Benha University, Moshtohour, Kalubeya

³ Virology Department, Animal Health Research Institute, Agricultural Research Center, Giza, Egypt

⁴ Department of Virology, Faculty of Veterinary Medicine, Benha University, Moshtoher, Benha, Egypt

10.21608/bvmj.2023.206881.1653

Abstract

Lumpy skin disease (LSD) is a financially significant Capripoxvirus-induced disease of cattle . Despite Egyptian veterinary officials' comprehensive immunisation programme, LSDV is still prevalent and widespread throughout the country. Understanding molecular epidemiology is critical for developing better LSD eradication and control measures.. The present research was designed to assess the genetic relatedness between newly strain from Menofia governorate 2020 and previously isolated field and vaccinal strains through molecular characterization and phylogenetic analysis. During the summer of 2020, ten skin tissue samples were taken from LSD-infected cattle that were involved in the epidemic. LSDVs were detected in clinical samples using PCR targeting the GPCR gene. By PCR, all ten samples tested positive for LSDV. One of these PCR-positive samples and the vaccinal strain were then amplified and sequenced for molecular characterization utilising a G-protein coupled chemokine receptor (GPCR) gene, ORF95 and ORF103. Phylogenetic analysis indicated that LSDV strain circulating in Egypt 2020 was closely related with sequences previously isolated from Egypt and that from neighboring countries. A comparison of the GPCR, ORF095, and ORF103 genes revealed that outbreak isolate dissimilar from strain included in vaccine. This information is essential for comprehending LSDV molecular epidemiology and to contribute to the Egyptian government's development strategies of control methods.

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