Synergistic antimicrobial activity of black pepper extract with some antibiotics combination on Escherichia coli isolated from chickens

Document Type : Original Article

Authors

1 Department of Bacteriology, Immunology and Mycology Fac. Vet. Med Benha University, Egypt

2 Department of Microbiology Fac. Vet. Med Zagazig University, Egypt

3 Department of Microbiology Animal Health Research institute Egypt

Abstract

Antimicrobial activity of methanol piper nigrum extract in combination with some antibiotics was investigated against
Escherichia coli isolated from chicken samples. The in-vitro antimicrobial sensitivity pattern of these isolates was done
and the intermediate isolates were checked for combination with methanol piper nigrum extract after detecting their
antimicrobial activities against these isolates by both disc diffusion test and MIC and the obtained data showed that
antimicrobial activities of amoxicillin, doxycycline, gentamicin, erythromycin and cefotaxime were enhanced in
combination with methanol piper nigrum extract. The obtained data showed that enhancement by both disc diffusion test
and MIC for methanol piper nigrum extract and each of the mentioned antibiotics in decimal assay of additivity ratio was
(0.5 for amoxicillin, cefotaxime and 0.5 for extract and 0.4 for doxycycline, gentamicin, and erythromycin and 0.6 for
extract) against E. coli isolates.

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