E. coli strains producing Shiga toxin in cattle carcasses at abattoir level

Document Type : Original Article

Authors

1 Department of food Hygiene, Fac. Vet. Med, Benha University

2 Food Hygiene Dept., Animal Health Research Institute Shebin El- Kom branch

Abstract

A total of one hundred random swab samples were collected from the cattle carcasses slaughtered at four different
abattoirs located in Menufia governorate namely A, B, C and D (25 of each). The sampling site was randomly taken from
each carcass inside the abattoir. The collected samples were examined for presence of E. coli and their virulence genes
using PCR technique. Incidence of E. coli isolated from the examined cattle swabs samples taken from abattoir A was E.
coli (O55: H7, O111: H2, O126 and O114: H21) 4 % for each, E. coli (O111: H2) was 4 % and E. coli (O126) 4%. While
in case of abattoir B E. coli (O86, O124 and O126) 4 % for all and E. coli (O119: H4 and O26: H11) 8% for both. On the
other hand, results of abattoir C was E. coli (O111: H2 8% and O26: H11 12%), E. coli (O86, O114: H21, O124 and
O142) 4% of each. But in case of abattoir D was E. coli O26: H11 16%, E. coli (O111: H2 O55: H7 and O126) 8% for
each, E. coli (O44: H18 and O125: H21) 4% for both. We found also that Shiga- toxin 1 gene (stx1) virulence gene was
present in O26: H11, O44: H18, O55: H7, O111: H2, O119: H4, O126 and O128: H2. On the other hand, Shiga- toxin 2
gene (stx2) virulence gene was present in O26: H11, O44: H18, O86, O111: H2, O114: H21, O119: H4, O125: H21 and
O142. While intimin gene (eae) virulence gene was present in O26: H11 and O111: H2. Also haemolysin gene (hylA)
gene was present in O26: H11, O55: H7 and O111: H2.

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