Traceability of enteropathogenic E. coli in cattle and camel carcasses

Document Type : Original Article

Authors

1 Food hygiene Department, faculty of veterinary medicine, Benha University

2 Animal Health Research Institute, Tanta Branch

3 Environment Management, Al Qaliubiya Governorate

Abstract

A total of 120 random samples of cattle and camel carcasses (60 0f each) were collected from the different abattoirs
located in Qaliubiya governorate. The samples taken from each carcass were represented meat, spleen, liver and kidneys
(15 samples of each), the samples were collected directly after slaughtering and evisceration. The collected samples were
subjected to bacteriological examination for detection and identification of E. coli. Enteropathogenic E. coli organisms
were 6.67%, 20%, 26.67%, and 40% of the examined samples of cattle meat, spleen, liver and kidney respectively and
were 0.0%, 13.33%, 13.33%, and 33.33% of the examined samples of camel meat, spleen, liver and kidney respectively.
The isolated strains were investigated by using Multiplex PCR to detect presence of virulent genes (stx1, stx2 and eaeA)
in each isolated Strain of E. coli. O15 Positive strain for stx2 gene, O26 & O111 Positive strains for stx1, stx2 and eaeA
genes. O44 & O126 Positive strains for stx1 gene. O55 Positive strain for stx2 gene and eaeA genes. O91, O103 & O127 Positive
strains for stx1 and stx2 genes O124 negative strains for stx1, stx2 and eaeA genes. The public health importance of the
isolated E. coli and the possible sources of contamination of cattle and camel carcasses with E. Coli as well as suggestive
hygienic measures to improve the quality of carcasses were discussed

Keywords

Benha Veterinary Medical Journal
Volume 31, Issue 1
September 2016
Pages 50-55

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