Baby Grivet Monkey Kidney (BGM) a new cell line for adaptation of fowl pox virus

F., El-Bagoury G.; M., El-Nahas E.; Nakhla, Olfat A.; Hassan, Nesma M.

doi:10.21608/bvmj.2016.31239

Baby Grivet Monkey Kidney (BGM) a new cell line for adaptation of fowl pox virus

Document Type : Original Article

Authors

¹ Department of virology, Faculty of Veterinary Medicine, Benha University, Egypt

² Veterinary Serum and Vaccine Research Institute, Abbassia, Cairo

10.21608/bvmj.2016.31239

Abstract

The need to economic, sensitive and easily maintained cell cultures use for massive production of fowl pox vaccine was
necessary. We adapted Fowl pox virus (FPV) Baudette strain on Primary chicken embryo fibroblast (CEF), Baby Grivet
Monkey Kidney cell line (BGM) and African green monkey kidney (Vero) cell lines. The highest virus titers were 10^6.5
after the 9^th passage in CEF and 10^6.2 after the 12^th passage on BGM while Vero cells were abortive to the virus. BGM
cells were selected for study cytopathic effect (CPE) and growth kinetic as it easily maintained than primary CEF cells.
The characteristic CPE were rounding till 6^th passage with intracytoplasmic inclusion by the 7^th passage and syncytium
formation appeared from the 9^th passage till the 13^th passage. The virus was highly cell associated for the first 84h post
inoculation(PI) with a titer of 10^4.5 then a maximum titer was 106.2 in cell free portion after 120h PI. In conclusion, BGM
were considered a new susceptible cell for growth of FPV with best harvesting time 120h PI to obtain a maximum titer
for subsequent vaccine production

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